Biotechnology Program Stem Cell Technology Certification

Paul A. Raju, D.Phil

Objective

Educate and train students with the specialized skills required for jobs in the lucrative and rapidly growing field of stem cell research

Launched in 2006

Course Structure

BTEC21A: Mammalian Cell Culture (6 weeks/9 hours per week)

BTEC21B: Fluorescent Cell Technology (6 weeks/9 hours per week)

BTEC21C: Stem Cell Technology (8 weeks/9 hours per week)

BTEC24 & 25: Introductory and Advanced PCR BTEC22: ELISABTEC23: Western Blotting

Pre-requisite - 1 semester of Biology with lab and 2 semesters of Chemistry including Organic with lab

Text - Culture of Animal Cells: A manual of basic technique (Ian Freshney)

Fall, Spring, and Summer. Day and Night Classes ~1 calendar year to complete

CCSF Cell Culture Facility

Instrumentation

2 - Flow Cytometers (BD FACScans)6 - Epifluorscence Inverted Microscopes10 - Inverted Microscopes6 - CO2 Incubators & 6 Laminar Flow Hoods

Main Skills Acquired

• Understand the principles of stem cell biology• Critically and independently assess the reality and future of applied stem cell biology• Differentiate between different types of stem cells in terms of their function, use and care• Isolate mesenchymal and hematopoietic stem cells from mouse bone marrow• Culture and differentiate mouse Embryonic Stem Cells• Understand (principles) and operate a flow cytometry• Analyze flow cytometry data

Internship Training - in various stem cell research labs in the SF Bay Area (UCSF, Genentech)

To date, over 150 students have received their certificates

Approximately half of our graduates have been hired at UCSF, Stanford, Gladstone Institutes, Stem Cell Inc., Geron, Progenitor, Genentech, etc

Evolution of the Program

2002 NSF-ATE funded “Fix a Gene”2004 Prop 71 - $3 billion for Stem Cell Research ( California Initiative for Regenerative Medicine, CIRM)2005 WIA CA Governor’s grant2005 Creation of Cell Culture Laboratory2006 Launching of Stem Cell Certificate Program2007 First human ES cell training at UCSF2009 CIRM Grant

A Novel Curriculum for Training in Stem Cell Research

NSF CCLI Grant 0920799: Workforce Development in STEM Cell Research

Christopher Thomas Scott, Stanford University, PI

Aims1) Develop a multidisciplinary undergraduate curriculum for community and four year colleges for workforce training in stem cell research.

2) Cross-disciplinary design: basic and applied science, lab skills, and ethics, law, and policy

3) Pilot first module in four colleges (SPRING, 2011)

4) Deploy suite of measurements (summative and formative assessements)

NSF CCLI Grant: Workforce Development in STEM Cell Research

Content Developers* and InstructorsDritan Agilou* Stanford UniversityPaul Ananda Raju City College of San FranciscoMariluci Blandon Middlesex Community CollegeJessica Byrne* Art and IllustrationKatayoun Chamany Eugene Lang CollegeLily Cheng San Francisco State CollegeEric Chiao* Roche BiosciencesTyler Cutforth* University of California, Santa CruzNicole Holthius* Assessment DevelopmentThorsten Schlager* Harvard UniversityCarin Zimmerman* City College of San Francisco

Year 1 Progress: > 80 hours of Instruction and Materials

1. Primers: Short topical introductions for students2. Slide Presentations: Fully-illustrated slide sets with teacher’s notes and image library3. Activities: Cross-topic, multi-week activities test essential concepts and reflective learning4. Integrated Laboratories: Fully immersive labs teaching essential skills in stem cell biology5. Assessment Suite: Embedded, formative, and summative measurements

NSF CCLI Grant: Workforce Development in STEM Cell Research

Acknowledgements

CCSFPhil Jardim

Kristin HershbellDr. Carin Zimmerman

Dr. Golnar AfsharDr. Alex ZambonDr. Jennifer Ng

Dr. Edith KaeuperDr. Wing Tsao

Dr. Carole ToebeDr. Elaine Johnson

UCSF/GladstoneDr. Mort Cowan

Ms. Elizabeth DunnDr. Bruce Conklin

Dr. Jennifer NgDr. Susan Fisher

Miltenyi BiotechMr. Thad Baker

Ms. Julie RaeDr. Shane Oram