CHROMATOGRAPHY AP BIO LAB #4A
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CHROMATOGRAPHYAP BIO LAB #4A
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PLANT PIGMENTS
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http://members.aol.com/diaspore/vhouse/biology/projects/photosynthesis/woida/meaning_of_light.html#question_1
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CHROMATOGRAPHYUSE PENCIL not pen, to
mark starting line !!!!!
Image by Riedell
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ADD PLANT PIGMENTS
Image from Biology Place: http://www.phschool.com/science/biology_place/labbench/lab4/deposit.html
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PLACE IN JARImage by Riedell
IMPORTANT:
Make sure tip of paper is touching solvent
Make sure pigment lineis ABOVE solvent
Put lid on jar !!!!!!!!
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Important
http://www.phschool.com/science/biology_place/labbench/lab4/pigsep.html
Don’t let solvent front run off end of paper !
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Marker Chromatography
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Rf =Distance pigment migratedDistance solvent front migrated
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PLANT PIGMENTSCarotene is orange yellow
Xanthophyll is lemon yellow
Chlorophyll a is blue green
Chlorophyll b is yellow green.
Image by Riedell
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Factors that affect movement of molecules during chromatography
1. ___________of molecule in solvent used(better it dissolves… farther it will move)
Solubility
SOLVENT used: Chlorophyll:
9:1 Petroleum ether : acetone
Markers: water
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Factors that affect movement of molecules during chromatography
2. _________of molecule(bigger molecules move slower)
Size
http://elvis.phys.lsu.edu/svoboda/post/figs/SumoWrestler.BMP
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Factors that affect movement of molecules during chromatography
3. _______________________ used (EX: Attraction of molecules to paper)
Attraction to media
http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/ch5_mol-seive.jpghttp://www.cofc.edu/~kinard/Applets/GasChromatographFID.gif
Lots of different kinds of chromatography
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Factors that affect movement of molecules during chromatography
4. __________allowed to runTime
http://www1.istockphoto.com/file_thumbview_approve/581259/2/istockphoto_581259_big_time.jpg
Longer time = More separation
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CHLOROPHYLL FLUORESCENCE
Image from Biology AP Edition by Campbell & Reece published by Pearson Benjamin Cummings