Chromatofocusingwith Polybufferand PBEHandbook

18-1009-07

Edition AB

Antibody PurificationHandbook18-1037-46

The Recombinant Protein HandbookProtein Amplification and Simple Purification18-1142-75

Protein PurificationHandbook18-1132-29

Ion Exchange ChromatographyPrinciples and Methods18-1114-21

Affinity ChromatographyPrinciples and Methods18-1022-29

Hydrophobic Interaction ChromatographyPrinciples and Methods18-1020-90

Gel FiltrationPrinciples and Methods18-1022-18

Handbooksfrom Amersham Pharmacia Biotech

Reversed Phase ChromatographyPrinciples and Methods18-1134-16

Expanded Bed AdsorptionPrinciples and Methods18-1124-26

Chromatofocusingwith Polybuffer and PBE18-1009-07

Microcarrier cell culturePrinciples and Methods18-1140-62

PolybufferTM and Polybuffer exchangers PBE 94 and PBE 118 have been speciallydesigned by Amersham Pharmacia Biotech for chromatofocusing. They areinexpensive and very simple to use.

This handbook is designed as an introduction to chromatofocusing and as a guideto the use of chromatofocusing products available from Amersham PharmaciaBiotech.

Fig. 1. Fractionation of soluble proteins extracted from elk muscle. Column C 10/40. Bed height: 35cm. Sample: 5 ml of supernatant from elk meat homogenate. Elution conditions: Start buffer 0.025 Methanolamine HCl, pH 9.4, elution buffer 0.0075 mmol/ pH unit/ ml Polybuffer 96, pH 6. Flow rate: 20cm.h-1.

Recorders REC 111, REC 112Reliable and accurate chart recorders for use with Pharmacia monitors or otherlaboratory equipment to give a permanent record of your experiments.

Correct column design is essential for the best results. Amersham Pharmacia Biotechprovide a wide range of column designs to suit different applications and budgets.The best results are obtained on a long narrow column. Useful column types arethe C10/40, SR 10/50, C16/70 and XK 16/70.

18-1110-91

Polybuffer, Pharmalyte, Sephadex and Sepharose are trademarks of Amersham Pharmacia Biotech Limited.

Amersham is a trademark of Amersham plc.

Pharmacia and Drop Design are trademarks of Pharmacia Corporation.

All goods and services are sold subject to the terms and conditions of sale of the company withinthe Amersham Pharmacia Biotech group that supplies them.

A copy of these terms and conditions is available on request.

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www.apbiotech.com

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