Chi to Plant in Agriculture
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Transcript of Chi to Plant in Agriculture
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
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ChiPro GmbH
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ChitoPlant Application Aspects of Chitosan in
AgricultureDr. Sebastian Thiem
ChiPro GmbH, BremenGermany
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
ChiPro GmbH - Business concept and idea
Cooperative development of natural and sustainable products
CosmeticsMedicine
Veterinary medicine
Agriculture
Textile
Biotechnology
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Product focus
Chitosan/Chitin Extracts Algae/Seaweed
Essential oils Herbs/Spices
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Properties and use of Chitosan
• Antimicrobial• Antiviral• Can form fibres and films• Anti-inflammatory• Immune stimulating• Promotes wound-healing• Elicitor
Cosmetic Applications
Medical Applications
Clothing
Agricultural Applications
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
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ChiPro GmbH
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• Plant strengthener• 99.9% Chitosan, 0.05% Boron, 0.05% Zinc• Yield increase• Resistance against pathogens• Healthier Plants
ChitoPlant
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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• Cell walls of funghi• Crabshells
Natural occurence of Chitosan
Demineralization, deprotenation
Deacetylation
Crab Shells
Chitosan
Chitin
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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What is Chitosan ?
• Kationic Biopolymer • Colorless• Amorph• High charge density• nontoxic, biodegradable
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Elicitor: A substance that triggers defense mechanisms in a plant.
Polysaccharides, small proteins, or lipids associated with fungal or bacterial cell walls, internal molecules, e.g. Salicylic acid, Jasmonates, Ethylene
Chitosan in agriculture - Elicitor function
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Chitosan naturally occurs in the cell wall of many funghi and thus is recognized by the plant as a „pathogen“ and can act as an exogeneous elicitor.
Chitosan in agriculture - Elicitor function
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Induction of plant resistance reactions
Taken from : David A Jones and Daigo Takemoto, Current Opinion in Immunology 2004, 16:48-62
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ChiPro GmbH
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• Complex and composed of multiple layers of defence:– Biochemical and genetical reactions, e.g.:
• Expression of Chitinases and Chitosanases Destruction of fungal cell wall
• Sythesis of Phytoalexins Antibiotic properties• Production of Hydrogenperoxide Toxic• Enhanced growth
– Physical and physiological reactions e.g.:• Reduced opening times of stomata Hinders intrusion of several funghi• Lignification of cell walls Increases physical barrier• Changes in ultrastructure of roots and leafes Hinders intrusion of
several funghi
Plant defense reactions
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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• ChitoPlant acts as an elicitor• ChitoPlant triggers plant resistance reactions
ChitoPlant strengthens the plants against infections with pathogens
ChitoPlant increases plant growth and root formation
ChitoPlant – Mode of action
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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ChitoPlant application in Strawberries
Yield increase
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Strawberry variety: Elsanta, 15-18cm Rhizome diameter, Frigo plants
Treatment regime: • Dipping the whole plant for 10 minutes in 0.05% ChitoPlant Solution directly before
planting• Band spraying 2 days after planting: 20cm width, 100ml 0.05% ChitoPlant solution per
meter• Leaf spraying from timepoint of sprouting: Dripping wet every 14 days until harvest,
0.05% ChitoPlant solution
Trials where done in four replicates.
EvaluationTotal yield increase compared to untreated control
Trial details
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
90.00
95.00
100.00
105.00
110.00
115.00
120.00
Total yield increase in %
KontrolleChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Field trial Landwirtschaftskammer Weser Ems 2003, Dr. R. Faby
200
210
220
230
240
250
260
Yield increase in g/Plant
KontrolleChitoPlant Behand-lung
Ertr
ag (g
/Pfla
nze)
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Field trial ChiPro GmbH, 2004
90.000
95.000
100.000
105.000
110.000
115.000
120.000
125.000
Total yield increase in %
KontrolleChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Field trial ChiPro GmbH, 2005
90.00
100.00
110.00
120.00
130.00
140.00
150.00
Total yield increase in %
KontrolleChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Field trial ChiPro GmbH, 2005
1. Ernte 2. Ernte 3. Ernte 4. Ernte90.00
100.00
110.00
120.00
130.00
140.00
150.00
160.00
170.00
180.00
Yield increase in % per harvest
KontrolleChitoPlant
Ertr
agss
teig
erun
g (%
)
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
ChitoPlant application in Strawberries
Protection against post-harvest diseases
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Experimental details:• Dipping of single strawberry fruits in 1% (w/v) ChitoPlant in destilled Water for 1
minute Control: Water, pH5.6 with 0,05 % (w/v) Triton X-100. • Air drying for 1 h• Storage of single fruits in plasticboxes for 3 days @ 20°C, 95-98% humidity • 5 repeats
Reference: Romanazzi G. , Santini M., Murolo S., Landi L., 2007. Antimicrobial and eliciting activity of chitosan in the control of gray mold of strawberries in storage. Proc. COST 924 "Novel approaches for the control of postharvest diseases and disorders", Bologna, 403-408 (European Cooperation in the field of Science and Technical Research)
ChitoPlant effect against Botrytis cinerea and Rhizopus stolonifer
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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ChitoPlant effect against Botrytis cinerea and Rhizopus stolonifer
Kontrolle Botrytis cinerea Rhizopus stolonifer0%
20%
40%
60%
80%
100%
120%
Reduction of mold infection by Botrytis cinerea and Rhizopus stolonifer (Gray mold)
Befa
ll in
%
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
ChitoPlant application in potatoes
Yield increase
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Potatoe variety: Cilena
Treatment regime: • Potatoes are sprayed with 0.05% ChitoPlant solution during
sowing with sowing machine• Trials have been performed according to EPPO guideline 1/32
(Rhizoctonia solani on potatoe)
EvaluationTotal yield increase compared to untreated control
Trial details
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Yield increase potatoes in dt/ha
299
338
430
489
397
469
376
432
0 100 200 300 400 500 600
--Control
1,5+4,0Risolex fl.+
Dithane NeoTec
342493
406414
1,5Risolex fl.
342505
479442
0,05ChitoPlant
SMK01E01dt/haNIK01E01dt/ha
WEK01E01dt/haAveragedt/ha
GD5%=69,8
GD5%=52,9
GD5%=54,2
Field trial Spiess Urania, 2002
Yield increase: 17,5%
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
90
95
100
105
110
115
120
Yield increase potatoes in %
Behandlung mit konventionellen ChemikalienChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Field trial Agrarfrost GmbH & Co.KG, 2002
Yield increase: 17 %
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Field trial University Rostock (organic), 2002
90
95
100
105
110
115
120
Yield increase potatoes in %
KontrolleChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Yield increase: 16 %
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Field trial University Newcastle (organic), 2002
90
95
100
105
110
115
120
Yield increase potatoes in %
KontrolleChitoPlant Behandlung
Ertr
agss
teig
erun
g (%
)
Yield increase: 18 %
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
ChitoPlant application in potatoes
Protection against diseases
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
your reliable partner in natural ingredients
Potatoe variety: Princess
Treatment regime: • Combined seed and leaf treatment• Potatoes are sprayed with 0.1% ChitoPlant solution during sowing• Spraying of leaves with 0.1% ChitoPlant solution every 10 days until harvest
Evaluation
Rating of infestation and severity of Phytophtora infestans, Streptomyces scabies and Rhizoctonia solani, respectively
Trial details
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Field trial University Rostock, 2002(Phytophtora infestans and Streptomyces scabies)
Krautfäulebefall in %0
10
20
30
40
50
60
70
Unbehandelte KontrolleBlattbehandlungPflanzgutbehandlungKombinierte Pflanzgut- und Blattbehandlung
Schorfbefall Boniturnote0
20
40
60
80
100
120
140
160
180
200
Unbehandelte Kon-trolleBlattbehandlungPflanzgutbehandlungKombinierte Pflanzgut- und Blat-tbehandlung
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Field trial University Kassel, 2006/7(Rhizoctonia solani)
0%
20%
40%
60%
80%
100%
10-15%
5-10%
1-5 %
0-1 %
0%
10-15% 12,4 0,9 11,2 15 9,4 6,6 1,6 1,7
5-10% 16,1 13,9 36,4 24,2 22,1 25 38,6 17,5
1-5 % 23,3 18,3 29,9 24,2 30,2 21,3 26,8 13,3
0-1 % 14,5 19,1 9,3 20,3 21,5 13,2 14,2 7,5
0% 33,7 47,8 13,1 16,3 16,8 33,8 18,9 60
Var7 Var4 Var3 Var2 Var5 Var6 Kontrolle Var8
Var. 4: 0,1% ChitoPlant
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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ChitoPlant application in Apples
Protection against diseases
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Trial detailsTrials are ongoing with University of Konstanz in the context of a governmental supported program against Erwinia amylovora (Fire blight).
Two in vitro testsystems:
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ChiPro GmbH
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Results from in vitro trials 2010
Trials are repeated 2011in vitro and in the field
Anne-Conway-Str. 9; Tel: +49-(0)421-27656923;
E-mail: [email protected] ;
D- 28359 BremenFax: +49-(0)421-27656920www.chipro.de
ChiPro GmbH
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Thank you !