Hybrid Integrated Circuit/Microfluidic Chips for the Control of Living Cells and UltraSmall
Chemistry on Ultrafast and Ultrasmall Scales Trevor Smith Ken Ghiggino Paul Mulvaney School of...
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Transcript of Chemistry on Ultrafast and Ultrasmall Scales Trevor Smith Ken Ghiggino Paul Mulvaney School of...
Chemistry on Ultrafast and Ultrasmall Scales
Trevor SmithKen GhigginoPaul Mulvaney
School of Chemistry,University of Melbourne
Ultrasmall - techniques
Confocal and multiphoton microscopy
Single molecule detection and emission spectroscopy FCS, antibunching Polymers & quantum dots
Total internal reflection fluorescence (EWIF)
Ultrasmall - facilities
Scanning confocal and multiphoton microscopy Time-resolved fluorescence imaging
LaVision PicoStar TCSPC (EI, Becker & Hickl, other)
Single molecule detection and emission spectroscopy
FCS, antibunching, single molecule spectroscopy (spectrograph/CCD)
Polymers & quantum dots Total internal reflection fluorescence (EWIF) Scanning near field (SNOM)
Ultrafast - techniques
Time-correlated single photon counting Time-resolved fluorescence anisotropy
Flash photolysis Pump-probe techniques
Time-resolved absorption Fluorescence upconversion Multi-pulse, multi-wavelength photon
echoe
Ultrafast - facilities
Nanosecond flash photolysis (Nd:YAG/OPO) ns gated CCD/spectrometer
Picosecond dye lasers Femtosecond Ti:sapphire systems
oscillator/OPO system Pulse picked/cavity dumped MHz rep. rates, nJ pulse energies, tuneable
amplified femtosecond Ti:sapphire oscillator/OPA <280 kHz rep. rates, µJ-10s of nJ pulse energies,
tuneable
Evanescent Waves
n1
n2
n1> n2
Standing wave
Total internal reflection(if i> c)
Evanescent waveE=Eoexp(-x/)
Refraction(if i< c)
IncidentBeam, Eo
Interface
Normal
i
EWIF spectroscopy EWIF-FCS Time-resolved EWIFS Time-resolved EWIF anisotropy Time-resolved EWIF microscopy
Expressions for polarisations perpendicular, s, & parallel, p, to plane of incidence:
In bulk solution:
In-plane:
Out-of-plane:
r t Izy t 1
2Iyy t Iyx t
Izy t Iyy t Iyx t
r t Iyy t Iyx t Iyy t Iyx t
rbulk Iyy (t) Iyx (t)Iyy (t) 2Iyx (t)
Polarised EWIF
z
x
y
TREWIF Anisotropy
0 10 20 30 40 50 60 70Time (ns)
BULK
= 64 nm
= 76 nm
= 113 nm
INCREASINGDISTANCE AWAY FROMINTERFACE
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
r(t)
N
H
S
O
3
H
“In-plane” and “Out-of-Plane” anisotropy
-0.1
-0.05
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0 20 40 60 80 100 120
Time (ns)
r(t)
Bulk900nm353nm260nm215nm188nm155nm135nm120nm
N
N
N
H
+
-0.1
-0.05
0
0.05
0.1
0.15
0.2
0 20 40 60 80 100 120Time (ns)
r(t)