Chem Project on ascorbic acid

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    Acknowledgement

    I would like to express my special thanks and gratitude to

    my teacher (PGT Chemistry) , who gave me the

    golden opportunity to do this wonderful proect, and gave

    me guidance during the process!

    I would also like to thank my parents who helped me andguided me!

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    CERTIFICATE

    This is to certify that the Chemistry proect "#TI$%TI&'

    & %#C&*IC %CI+ I' CIT# IT# is a -ona fide

    work of, ur name studying in class .II/* having

    C!*!#!" roll no! ! It doesn0t indulge in

    plagiarism of any kind!

    Teachers name

    PGT, Chemistry

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    AIM OF THE PROJECT

    The aim of this proect is to investigate the

    ascor-ic acid content in different citrus fruits!

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    APPARATUS AND MATERIALS

    REQUIRED

    1! ollowing freshcitrus fruit uices2

    a! 3emon uice

    -! $osam-i uice

    c! %mla uice4! 5!1 $ 'a&6

    7!*urette

    8! 15 ml graduated cylinder

    9! Titration flask

    :! 455 ml -eaker

    ;! *urette stand

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    THEORY

    CITRUS FRUITS

    Citrusis a common term and genus(Citrus) of flowering

    plantsin the ruefamily, utaceae! The -est known examples

    are oranges, lemons, limes, amla, etc! They are a rich source

    of two maor acids, citric acidand ascorbic acid.

    RELATIE CONTENT IN CITRUS FRUITS

    #ince citrus fruits have -oth citric and ascor-ic acids, -oth

    acids will collectively neutrali>e 'a&6! *ut, ascor-ic acid

    content is much higher than citric acid in many citrus fruits

    (ascor-ic acid content in lemon is a-out 74? while citric

    acid content is 9/:?)! 6ence, contri-ution to titration with

    'a&6 will -e more for ascor-ic acid!

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    CITRIC ACID

    Citric acid is a weak organic acid with the formula C6H8O7!

    It is a natural preservative/conservativewhich occurs

    naturally in citrus fruits and is also used to add an acidic or

    sour taste to foods and drinks! In -iochemistry, the conugate

    -ase of citric acid, citrate, is important as an intermediate in

    the citric acid cycle, which occurs in the meta-olism of allaero-ic organisms! It consists of 3 carboxyl (-COOH

    !roups contri-uting to its acidic character.

    "olar #ass$ %&'.%' ! #ol-%

    asicity$ 3

    )tructure$

    ASCOR!IC ACID

    %scor-ic acid ((*-3+,-dihydroxy--(()- %+'-

    dihydroxyethylfuran-'(H-oneis a naturally occurring

    weak, organic acid with formula C6H8O6! It is a white solid

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    and water solu-le! It is a form of ita#in C! It is optically

    active and 3/ascor-ic acid is naturally occurring and has a

    specific rotation of '3o! It consists of ' alcoholic !roups (-

    OH contri-uting to its acidic character! It is classified as a

    reductone!

    "olar "ass$ %76.%' ! #ol-%

    asicity$ '

    )tructure$

    IMPORATNCE OF ITAMIN C

    @itamin C plays a very important role in the maintenance of

    the human -ody! It is reAuired in very small amounts !$any

    animals produce vitamin c themselves, nut humans do not!

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    It helps in the formation of colla!en, the most a-undant

    fi-re in connective tissues!

    It catalyses healing of all wounds! rom -roken -ones

    to cuts to surgical wounds, @itamin C is taken orally! It makes the headlines when it comes to cancer

    prevention! Its antioxidant properties protect cells from

    damage and mutations!

    It also prevents cataract and heart diseases due to its

    a-ility to counteract free radicals!+% for @itamin C is :5/=5mg!

    %sthmatic and dia-etic patients need larger doses

    (a-out 1/7 g) of it!

    TITRATION% titration is a techniAue where a solution of known

    concentration is used to determine the concentration of an

    unknown solution! Typically, the titrant (the known solution)

    is added from a -urette to a known Auantity of the analyte

    (the unknown solution) until the reaction is complete!

    Bnowing the volume of titrant added allows the

    determination of the concentration of the unknown! &ften,

    an indicator is used to usually signal the end of the reaction,

    the endpoint!

    The unknown concentration is found out -y law of

    eAuivalents i!e!

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    0%%10''

    here ' is normality of solution and @ is the volume

    reacted!

    The eAuation can also -e written as2

    n%"%%1n'"''

    6ere n is the Dn factor0 of the compound and $ is the

    molarity of the solution! &nly $1 is unknown here!

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    TITRATION OF CITRUS FRUIT

    JUICE "ITH N#OH

    6ere 9 ml of respective uice will -e titrated with 5!1 $

    'a&6!

    '&T"2 Euice should -e freshly prepared and have minimum

    pulp, if any!

    *eaction2

    C:6

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    LEMON JUICE

    &*#"@%TI&' T%*3"

    ).0O 40454

    *940: ("

    ;40

    *940: ("

    5O5

    *940: ("

    1 5 9; 9;

    4 5 99 997 5 99 99

    Concordant readin! 1 #l

    CC

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    MOSAM!I JUICE

    &*#"@%TI&' T%*3"

    ).0O 40454

    *940: ("

    ;40

    *940: ("

    5O5

    *940: ("

    1 5 < ust pin!

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    !I!LIO$RAPHY

    ikipedia

    6owstuffworks!net

    Comprehensive Chemistry 3a- $anual