Chem 217

Metallobiochemistry T/Th 8-9:20AM, W 12-12:50

Pat Farmer

PSI 319

x6079, pfarmer@uci.edu

•Office hours:– MW 8-9AM and by appointment

OverviewOverview

Emphasis will be on the structural motifs of metalloprotein centers and their associated reactivities. Course material will offer broad exposure to multidisciplinary topics and methodologies. Prerequisites include undergraduate inorganic chemistry, physical or analytical chemistry, some knowledge of biochemistry. The major goal of this course is to provide you with the background and tools necessary for a critical appraisal of current bioinorganic topics in the literature. One research project will be required, given as a 3-5 page

report at the end of the term.

Understanding Protein Structure

Looking at Protein Images– what do those squiggles mean

Downloading Structures

from the PDB– something you can do

through out the course

Imaging an Active Site in the Modeling Facility– make an image and see it on the web

Bioinorganic Motifs• Hemeproteins,

– the colors of life • FeS clusters,

– from a time before oxygen• Cu proteins,

– versatile redox centers• Zn, Ca

– signal transducers

Heme Proteins: The colors of life

– “Why the bloode is red and the grasse green, these are mysteries no Man can fathom” John Dunne 17xx?

heme are ubiquitous

– one structure/many functions

– function controled by • oxidation state• ligand binding

FeS clusters: an ancient motif

ubiquitous

in mitochondria self-assembly

of clusters optimized for fast electron transfer

– minimum reorganization– electron delocalization

Cu Proteins:

Controlled Redox– oxygen binding and activation– cyt c oxidase, THE ultimate

electron acceptor

Radical Generation – tyrosinase

Signal Transduction

Ca binding proteins– EF hand motif

• intercellular

signal amplification

Zn binding proteins– Zn fingers– DNA binding

• gene regulation

Procedures

Highlight any procedural differences from regular projects of this type

Discuss requirements, benefits, and issues of using new procedures

List location or contact for procedures document (or other related documents) here

FOR MORE INFO...

Schedule

Review high-level schedule milestones here

List location or contact for detailed schedule (or other related documents) here

FOR MORE INFO...

Current Status

High-level overview of progress against schedule– On-track in what areas– Behind in what areas– Ahead in what areas

Unexpected delays or issues

Related Documents

marketing plan– location or contact name/phone

budget– location or contact name/phone

post mortem– location or contact name/phone

submit questions– location or contact name/phone