CHEM 213 Experiment: Column Chromatographychemistry.bd.psu.edu/justik/CHEM 213/CHEM 213...
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CHEM 213 Experiment: Column Chromatography
Transcript of CHEM 213 Experiment: Column Chromatographychemistry.bd.psu.edu/justik/CHEM 213/CHEM 213...
CHEM213Experiment:ColumnChromatography
Procedure:Youwillbeseparatingthemixturethatresultsfromthereactionbelow:
Inthisparticularexperiment,theoxidationisincompleteandyouhaveamixtureoffluoreneandfluorenonetoseparate.Objectives:
• Separatethetwocomponentsbycolumnchromatography.• Identifyeachcomponent
• Determinethemassofeachcomponent
Step1:Weighout~50mgofthemixture.Useabalanceoutsideofhoodtoeliminatetheindicatedmassfrombeingaffectedbytheairflow.FollowtheprocedurefromWilliamsonasfollows: