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    CHECKLIST BIOLOGY PRACTICAL (BIOLOGY A+ DI HATIKU)

    CHP NO. AIM OF THE EXP+

    HYPOTHESIS +

    MATERIALS&APPARATUS+

    DIAGRAM

    MAGIC BOX PROCEDURE:

    K1 : Preparation of material ! apparat"(#K1)

    K$ : Operatin% fi&e' ariale

    K* : Operatin% manip"late' ariale

    K#: Operatin% repon'in% ariale

    K+ : Pre,a"tion (K+)4K1, K2, K3, K4, & K! 3 MARKS

    RESULT:* *-1

    "2##$%

    2'

    STUDYING THE MO(EMENT

    OF SUBSTANCES ACROSS

    THE PLASMA MEMBRANE

    AIM:

    To t"'. t/e fa,tor infl"en,e t/e

    'iff"ion of "tan,e t/ro"%/ a

    emi0 permeale memrane-HYPOTHESIS:

    T/e 'iff"ion of mole,"le t/ro"%/

    emi0permeale memrane i ae'

    on t/e ie of mole,"le-MATERIALS&APPARATUS:

    Bene'i,t2 ol"tion3 14 Star,/

    "penion3 Io'ine ol"tion3 *54

    %l",oe ol"tion3 i6in% t"in% an'

    ,otton trea'3 tet t"e3 ea6er3 an'

    B"nen "rnerDIAGRAM:

    78 R8 C8

    CO9CRETE T.pe o f

    ol"tion

    (%l",oe

    an' tar,/

    "penion)

    C/an%e in

    Color of

    ol"tion re"lt

    of Bene'i,t2

    tet

    8ol"me of

    %l",oe ol"tion

    (1+ml)

    8ol"me of

    tar,/

    "penion (1+

    ml)

    ABSTRACT Sie of t/e

    mole,"le

    in t/e

    i6in%

    t"e

    ;iff"ion of

    "tan,e

    t/ro"%/ a emi0

    permeale

    memrane

    7ETHO;S

    Oo

    t.pe of

    ol"tion

    (%l",oean' tar,/

    "penion)

    )R("TECHNI

    UE'

    Observe and

    recordt/e,/an%e in ,olor

    of ol"tion

    ini'e t/e

    i6in% t"e an'

    t/e ea6er re"lt

    of Bene'i,t2

    tet

    Fixed t/e

    8ol"me of

    %l",oe ol"tion

    to 1+ml8ol"me of

    tar,/

    "penion 1+

    ml) . using

    measuring

    cylinder

    *R("TECHNI

    UE'0

    1- Soa6 t/e i6in% t"in% in >ater for + min to

    often it- (K1)

    $- Tie one en' of i6in% t"e to preent

    lea6a%e-(K+)

    *- it/ 'itille' >ater- (K1)(K+)

    ?- 7i&e' io'ine an' 'itille' >ater- Oere'

    t/e ,olor(K1)(K#)

    @- Immere' t/e i6in% t"e into ea6er for #5

    min"te-(K1)

    - After #5 min"te3 oere an' re,or' t/e

    ,/an%e in ,olor of ol"tion ini'e t/e

    i6in% t"e an' t/e ea6er(K#)(K*)

    - Carr. o"t t/e Bene'i,t2 tet for ot/ ol"tionini'e t/e i6in% t"e an' t/e ea6er(K#)

    R-/:Con

    tent

    Ori%i

    nal

    Color

    ater3 ,otton t/rea'3 retort tan'3

    ,apillar. t"e3 r"ler3 mar6er pen3,ior3 $+5ml ea6er3 .rin%e3

    top>at,/ an' i6in% t"in%-

    DIAGRAM:

    78 R8 C8CO9CRETE T.pe o f

    "tan,e

    (>ater an'

    ",roe)

    In,reae t/e

    leel of ",roe

    ol"tion in

    ,apillar.

    eer.15 min"te

    for #+ min"te

    ,on,entration of

    ",roe ol"tion

    Time ta6en for

    mar6in% t/e

    leel of ",roe

    ol"tion in

    ,apillar. t"e at

    15 min"teinteral for #+

    min"te

    ABSTRACT Sie of t/e

    mole,"le

    Rate of

    ;iff"ion of"tan,e

    t/ro"%/ a emi0

    permeale

    memrane

    7ETHO;S

    Oo

    'ifferent

    ol"tion

    >/i,/ are

    'itille'

    >ater

    (ini'e t/e

    ea6er)an'

    *54

    ",roe

    ol"tion in

    t/e 8i6in%

    t"e

    -

    )R("TECHNI

    UE'Measure and

    recordt/e

    in,reae t/e

    leel of ",roe

    ol"tion in

    ,apillar.

    eer.15 min"te

    for #+ min"te

    by using ruler.

    it/ 'itille' >ater- (K1)(K+)

    ?- Clamp t/e ,apillar. t"e erti,all. to retort

    tan'- (K1)

    @- Immere' t/e i6in% t"e into ea6er

    ,ontainin% t/e 'itille' >ater-(K1)

    - 7ar6 initial leel of ",roe ol"tion (K1)

    - 7ar6 t/e leel of ",roe ol"tion eer.15

    min"te for #+ min"te (K*)

    15-Measure and recordt/e in,reae t/e leel of

    ",roe ol"tion in ,apillar. eer.15 min"te

    for #+ min"te . "in% r"ler-(K#)

    R-/:

    Time

    (min"te)

    T/e In,reae t/e leel of ",roe

    ol"tion in ,apillar. (mmmin01)

    5

    15

    $5*5

    #5

    Score 50/50

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    (mm)time(min)

    =nit0(mmmin01)

    *-*"2##0

    %1'

    STUDYING THE EFFECT OF

    HYPOTONIC. HYPERTONIC

    ANS ISOTONIC SOLUTION

    ON ANIMAL CELL

    AIM:To t"'. t/e effe,t of 'ifferent

    ,on,entration of o'i"m ,/lori'e

    (9aCl) ol"tion on re' loo' ,ell >it/in

    *5 min"te-

    HYPOTHESIS:T/e /i%/er t/e ,on,entration of o'i"m,/lori'e ol"tion t/e /i%/er t/e n"mer

    of ,renate' re' loo' ,ell

    -MATERIALS&APPARATUS:/i,/ are

    5-1+ 73

    5-*5 73

    5-#+ 7 an'

    5-?5 7

    )R("TECHNIUE'Count and

    record t/e

    n"mer of

    ,renate' re'

    loo' ,ell-

    Used the same'"ration of time

    >/i,/ i 15

    min"te for

    immerion of

    t/e re' loo'

    ,ell . "in%

    t/estopwatch.

    *R("TECHNI

    UE'0

    1- Lael # li'e >it/ A3 B3 C3 an' ;- (K1)

    $- P"t a 'rop of 5-1+ 7 o'i"m ,/lori'e on

    li'e A- (K1) (K$)

    *- Coer t/e li'e >it/ ,oerlip- (K1)

    #- P"t one 'rop of loo' (RBC) on t/e ot/er

    i'e of li'e A- (K$)

    +- Pla,e t/e filter paper on t/e oppoite i'e-

    (K1)(K+)

    ?- After 15 min"te3 ,o"nt an' re,or' t/e

    n"mer of ,renate' re' loo' ,ell- (K#)

    @- Repeat t/e tep 10@ >it/ 'ifferent,on,entration of o'i"m ,/lori'e ol"tion

    >/i,/ are 5-*5 73 5-#+ 7 an' 5-?5 7-(K*)

    R-/:C)/*/ 5

    6-7 )86

    -/ "M'

    N-79 5 )*/6

    6 96 )

    "A'#.1

    "B'#.3#

    "C'#.4

    "D'#.#

    *-# STUDYING THE EFFECT OFHYPOTONIC. HYPERTONIC

    ANS ISOTONIC SOLUTION

    ON PLANT CELLS

    AIM:To 'etermine t/e effe,t of 'ifferent

    ,on,entration of a ol"tion on t/e ma

    /ape of potato-

    HYPOTHESIS:T/e 8;8 ,on,entration of t/e ",roe

    ol"tion3 t/e /i,/ are

    5-$43

    +-5431+-5

    43 an'

    $+-54

    )R("TECHNI

    UE'Measure and

    recordt/e

    ,/an%e in maof potato ti"e

    in 'ifferent

    ,on,entration of

    ",roe ol"tion

    . "in%

    alan,e

    weighing scale

    Fixed the same

    '"ration of

    oa6in% time

    >/i,/ i ?5

    min"te forimmerion of

    t/e potato .

    "in% t/e

    stopwatch./

    Use the same

    ol"me of

    ",roe ol"tion

    >/i,/ i $5 ml

    by using

    measuring

    cylinder.

    *R("TECHNI

    UE'

    0

    1- Bore,"t o"t a potato to %et potato trip of

    t/e ame ie an' >ei%/ t/em o a to %et

    potato trip of t/e ame ma(K1)

    $- it/ $5 ml of ",roe

    ol"tion of 5-$43 +-543 1+-543 an' $+-54

    (K1) (K*)(K$)

    *- P"t* of potato trip of t/e ame >ei%/t in

    ea,/ ea6er (K1)

    #- Leae t/e potato trip immere' in t/e

    ",roe ol"tion for 1 /o"r(K$)

    +- (After 1 /o"r) ta6e o"t t/e potato trip from

    ea,/ ea6er3 'r. t/em "in% filter paper(K1)(K+)

    ?- 7ea"re an' re,or' t/e ,/an%e in ma of

    potato ti"e in 'ifferent ,on,entration of

    ",roe ol"tion . "in% alan,e >ei%/in%

    ,ale(K1)(K#)

    @- Repeat tep 10? t/ree time to %et a,,"rate

    'ata (K+)

    - Plot a %rap/ of t/e ,on,entration of ",roe

    ol"tion a%aint ,/an%e in ma of potato

    trip

    - Ta"late t/e 'ata in t/e tale elo> (K1)

    R-/:C)

    /*/

    5

    -)

    -/

    "='

    C8*; 7* 5 >/*/

    /> %;

    #.2 1t

    rea'

    in%

    $n'

    rea'i

    n%

    *r'

    rea'i

    n%

    Ae

    ra%e

    .#

    1.#

    2.#

    ;ifferent ,on,entration of

    ",roe ol"tion

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    *-+"2##

    %2'

    "2##

    %2'

    DETERMINING THE

    CONCENTRATION OF

    EXTERNAL SOLUTION

    ?HICH IS ISOTONIC TO THE

    CELL SAP OF A PLANT

    CELLS

    AIM:to 'etermine t/e ,on,entration of t/e

    ol"tion >/i,/ >ill maintain t/e ma of

    plant ti"e-

    HYPOTHESIS:T/e #.4 M",roe ol"tion >ill

    maintain t/e ma of t/e plant ti"e-

    T/e ,on,entration of ",roe ol"tion

    t/at remain len%t/ma of plant ti"e

    i iotoni,-

    MATERIALS&APPARATUS:

    Plant ti"e (Ram"tan)38ario"

    ,on,entration of ",roe

    ol"tion3e&ample: 5-173 5-$7 3 5-#73

    1-57 an' 1-+7at,/3

    Balan,e>ei%/in% ma,/ine-

    DIAGRAM:

    78 R8 C8CO9CRETE Con,entrati

    on of

    ",roe

    ol"tion-

    C/an%e in ma T.pe of plant

    ti"e3 ol"me

    of ol"tion3

    oa6in% time3

    ie of potato-

    ABSTRACT 0 0

    )R("TECHNI

    UE'

    Measure and

    recordt/e,/an%e in ma

    of plant ti"e

    in 'ifferent

    ,on,entration of

    ",roe ol"tion

    . "in%

    alan,e

    weighing

    machine.

    ",roe ol"tion

    >/i,/ i $5 ml

    by using

    measuring

    cylinder.

    *R("TECHNI

    UE'

    0

    1- ei%/ t/e ram"tan ti"e an' re,or' t/e

    initial ma(K1)(K#)

    $- it/ 155ml ",roe

    ol"tion of 5-173 5-$7 3 5-#73 1-57 an'

    1-+7(K1) (K*)(K$)

    *- P"t * of plant ti"e in ea,/ ea6er (K1)

    #- Leae t/e plant ti"e immere' in t/e ",roeol"tion for 1 /o"r(K$)

    +- (After 1 /o"r) ta6e o"t t/e plant ti"e from

    ea,/ ea6er3 'r. t/em "in% filter paper(K1)

    (K+)

    ?- 7ea"re an' re,or' t/e ,/an%e in ma of

    potato ti"e in 'ifferent ,on,entration of",roe ol"tion . "in% alan,e >ei%/in%

    ma,/ine-(K1)(K#)

    @- Plot a %rap/ of t/e ,on,entration of ",roe

    ol"tion a%aint ,/an%e in ma of plant ti"e

    (K#)

    R-/:C)

    /*/

    5

    -)

    -/

    "M'

    C8*; 7* 5 >/*/

    /> %;

    1t

    rea'

    in%

    $n'

    rea'i

    n%

    *r'

    rea'i

    n%

    Ae

    ra%e

    #.1

    #.2

    #.4

    1.#

    1.

    # #-*"2##0%

    2'

    STUDYING THE EFFECT OF

    TEMPERATURE ON

    SALI(ARY AMYLASE

    ACTI(ITY

    AIM:To ineti%ate t/e effe,t of temperat"re

    on aliar. am.lae a,tiit.-

    HYPOTHESIS:A t/e temperaturein,reae3 t/e rate of

    reaction catalysed by amylase in,reae"ntil optim"m temperat"re

    MATERIALS&APPARATUS:Saliar. Am.lae ol"tion3 14 Star,/

    S"penion3

    Io'ine ol"tion3 ;itille' >ater3

    Tet t"e ea6erD Stop>at,/3 'ropper3

    >/ite tile >it/ %rooe3 Gla ro'

    DIAGRAM:

    78 R8 C8CO9CRETE Temperat"r

    e

    -

    T/e time ta6en

    for t/e l"e

    la,6 ,olo"r of

    io'ine to

    'iappear (min)

    8ol"me of

    tar,/

    "penion

    (+ml)

    8ol"me of

    ABSTRACT 0 Rate of rea,tion

    ,atal.e' .

    aliar.

    am.lae-

    7ETHO;S

    O

    */-

    "C'

    T8 /7 /*@ 5 /8

    9- 9*)@ )- 5

    6 / 6*>>*

    "M'

    R*/ 5 7 *)//

    "71'

    15C

    $5C

    *5C

    #5C

    1- Laelle' tet t"e a A13 A$3 B13B$3 C13 C$

    3;1an' ;$ (K1)

    $- + ml of 14 tar,/ "penion are po"re' into

    t/e tet t"e A13 B13 C1 an' ;1 "in%

    .rin%e-(K1)(K$)

    *- $ ml of 5-14 am.lae i a''e' to tet t"e

    A$3 B$3 C$ an' ;$(K1)(K$)

    #- T/e tet t"e are immere' in >ater at/ at

    'ifferent temperat"re for + min"te-(K*)

    A1 an' A$ : 15C 3

    B1 an' B$ : $5C3C1 an' C$ : *5C3

    ;1 an' ;$ : #5C+- ;rop of io'ine ol"tion are a''e' eparatel.

    onto t/e %rooe of a >/ite tile "in% a

    .rin%e-(K1)

    ?- After + min"te3 po"r tar,/ "penion (A1)

    to am.lae ol"tion (A$) (K1)

    @- Stirre' t/e mi&t"re . "in% t/e %la ro' (K1

    - Stop>at,/ i a,tiate' imme'iatel. at 5

    min"te-(K+)

    - A 'rop of mi&t"re i tete' >it/ io'ine

    ol"tion on t/e >/ite tile- At eer. amplin%

    t/e 'ropper m"t e rine' >it/ ,lean 'itille'

    >ater-(K+)

    15- T/e tep i repeate' eer. min"te for

    15 min"te "ntil t/e mi&t"re top t"rnin%

    l"e la,6 in ,olo"r >/en tete' >it/ io'ine

    ol"tion-

    11- Measure and record t/e time ta6en for

    t/e l"e la,6 ,olo"r of io'ine to 'iappear .

    using a stopwatch in t/e tale-(K#)1$- Step +011 are repeate' >it/ tet t"e

    B3C3 an' ;-(K*)

    1*- Ta"late t/e 'ata in t/e tale

    elo>(K1)

    11- A %rap/ of t/e rate of am.lae a,tiit. a%ain

    temperat"re i plotte'-(K#)

    +7LPA

    Ram"tan

    ;ifferent

    ,on,entration of

    ",roe ol"tion

    Peele' ram"tan

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    #-# STUDYING THE EFFECT OF>H ON PEPSIN ACTI(ITY

    AIM:To ineti%ate t/e effe,t of pH on pepin

    a,tiit.-

    HYPOTHESIS:An a,i'i, me'i"m at pH * i optim"m

    for t/e a,tiit. of en.me-

    MATERIALS&APPARATUS:Al"min "penion3 14 pepin3

    5-173/.'ro,/lori, a,i'3 5-1 7o'i"m/.'ro&i'e ol"tion an' 'itille' >ater-3

    Tet t"e3 ea6er3 t/ermometer3 +ml

    .rin%e3 pH paper 3a >ire %a"e

    Stop>at,/3 'ropper3 a B"nen "rner3 a

    tripo' an' a tet0 t"e ra,6-

    DIAGRAM:

    78 R8 C8CO9CRETE pH of

    me'i"m

    (a,i'0 pH*3

    ne"tral0

    pH@3 an'

    al6aline0

    pH)

    T/e ,on'ition

    of t/e mi&t"re

    at t/e e%innin%

    an' after $5

    min"te t/e

    ,larit. of t/e

    ol"tion

    8ol"me of

    al"min

    "penion

    (+ml)3ol"me

    (1ml) an' (14)

    ,on,entration of

    pepin ol"tion

    an' temperat"re

    of me'i"m

    (*@C)

    ABSTRACT 0 Rate of rea,tion

    ,atal.e' .pepin- Pepin

    a,tiit.

    7ETHO;S

    Oin% ol"tion into ea,/ tet

    t"e:-(K1)(K*)P : 1 ml of 5-1 7 /.'ro,/lori, a,i' 1 ml of

    14 pepin ol"tion 3

    F : 1 ml of 'itille' >ater 1 ml of 14

    pepin ol"tion 3

    R: 1 ml of 5-1 7 of o'i"m /.'ro&i'e 1 ml

    of 14pepin ol"tion 3#- ;ip a pie,e of pH paper into ea,/ tet -(K1)

    +- T/e tet t"e are immere' in >ater at/ at

    *@C temperat"re for $5 min"te-(K$)

    ?- Observe and record t/e ,on'ition of t/e

    mi&t"re at t/e e%innin% an' after $5 min"te

    (K#)

    @- Calculate and recordt/e rate of reaction

    ,atal.e' . pepin . "in% t/eformula

    ! / time-

    R-/:Te

    t

    t"

    e

    pH 7i&t"re Rate of

    rea,tion

    ,atal.e'

    . pep

    (min01)-

    At t/ee%inni

    n%

    After$5

    min"t

    e

    P *

    F @

    R

    #-+ STUDYING THE EFFECT OFSUBSTRATE

    CONCENTRATION ON

    SALI(ARY AMYLASE

    ACTI(ITY

    "2##%1 EFFECT OF

    CONCENTRATION OF

    ALBUMIN SUSPENSION ON

    THE RATE OF REACTION OF

    PEPSIN ENYME'

    AIM:To ineti%ate t/e effe,t of "trate

    ,on,entration on aliar. am.lae

    a,tiit.-

    HYPOTHESIS:T/e /i%/er t/e concentration of

    substrate3 t/e /i%/er t/e rate of reaction

    catalysed by salivary amylase "ntil it

    rea,/e t/e ma&im"m rate-

    MATERIALS&APPARATUS:

    5-14 Saliar. Am.lae ol"tion3

    'ifferent ,on,entration of tar,/

    "penion

    (5-1435-$435-*435-#43 5-+4 an' 5-?4)

    Io'ine ol"tion3 ;itille' >ater3

    Tet t"e ea6erD Stop>at,/3 'ropper3

    >/ite tile >it/ %rooe3 Gla ro'

    DIAGRAM:

    78 R8 C8CO9CRETE Con,entrati

    on of

    tar,/

    "penion

    (5-1435-$4

    35-*435-#4

    3 5-+4 an'

    5-?4)

    T/e time ta6en

    for t/e l"e

    la,6 ,olo"r of

    io'ine to

    'iappear (min)

    Temperat"re

    (*@C)

    ol"me ($ml)

    an' (5-14)

    ,on,entration of

    aliar. am.lae

    ol"tion

    ABSTRACT 0 Rate of rea,tion

    ,atal.e' .

    aliar.

    am.lae-

    7ETHO;S

    O>*

    "7'

    R*/ 5 7 *)//

    "71'

    5-143

    5-$4

    5-*4

    1- Laelle'1$tet t"e a A13 A$3 B13B$3 C13

    C$ 3;13 ;$3 E13 E$3 in% tet

    t"e . "in% .rin%e-(K1)(K*)

    A1: 5-14 tar,/ "penion 3

    B1: 5-$4 tar,/ "penion3

    C1: 5-*4 tar,/ "penion3

    ;1: 5-#4 tar,/ "penion3

    E1: 5-+4 tar,/ "penion3

    ater at/ at

    temperat"re *@C for + min"te-(K1)(K$)+- ;rop of io'ine ol"tion are a''e' eparatel.

    onto t/e %rooe of a >/ite tile "in% a

    .rin%e-(K1)

    ?- After + min"te3 po"r tar,/ "penion (A1)

    to am.lae ol"tion (A$) (K1)

    @- Stirre' t/e mi&t"re . "in% t/e %la ro' (K1

    - Stop>at,/ i a,tiate' imme'iatel. (5

    min"te)-(K+)

    - A 'rop of mi&t"re i tete' >it/ io'ine

    ol"tion on t/e >/ite tile- At eer. amplin%

    t/e 'ropper m"t e rine' >it/ ,lean 'itille'

    >ater-(K+)

    15- T/e tep i repeate' eer. min"te for 15

    min"te "ntil t/e mi&t"re top t"rnin% l"e

    la,6 in ,olo"r >/en tete' >it/ io'ine

    ol"tion-

    11- Measure and record t/e time ta6en for

    t/e l"e la,6 ,olo"r of io'ine to 'iappear .

    using a stopwatchin t/e tale-(K#)(K1)1$- Step +011 are repeate' >it/ tet t"e

    B3C3 ;3 E an'

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    5/14

    5-#4

    5-+4

    5-?4

    ? ?-1"SPM

    2##%

    1'

    DETERMINING THE ENERGY

    (ALUE IN FOOD SAMPLES

    AIM:To 'etermine an' ,ompare t/e ener%.

    ,ontent in >/ite rea' an' pean"t-

    HYPOTHESIS:Pean"t pro'",e a lot of /eat ener%.

    /ite rea' pro'",e alittle /eat ener%.Pean"t pro'",e a

    /i%/er in,reain% in temperat"re ener%.

    al"e /8*>/ite rea'-

    MATERIALS&APPARATUS:;itille' >ater3 a pean"t3

    rea'3plati,ine3 an' ,otton >ool-

    Boilin% t"e3 t/ermometer3 retort tan'3

    a pin3 mea"rin% ,.lin'er3 B"nen

    "rner an' ele,troni, alan,e-

    DIAGRAM:

    78 R8 C8CO9CRETE ater

    temperat"re .

    "in% t/e

    thermometer

    Fixed t/e

    'itan,e

    et>een t/e

    oilin% t"e an'

    foo' ample

    >/i,/ i $,m .

    "in% t/eruler

    *R("TECHNI

    UE'

    calculate and

    record t/e

    ener%. ,ontent in

    foo' ample .

    "in% t/e

    form"lae elo>

    Energy value =

    Mass of$ater%g& '

    %(.#)g *!+C*!& '

    increase in

    ,emperature%C)

    Mass of food

    %g&-!

    =nit (6%01)

    Tale:

    !. ei%/ t/e pean"t an' re,or' it

    >ei%/t(K1)#. @ /8 >*-/ 57 */ /8

    6 5 /8 > */).

    0. ater

    melon

    MATERIALS&APPARATUS:

    ;CPIP Sol"tion3 5-14 aori, a,i'

    it/ nee'le3 Spe,imen

    t"e

    DIAGRAM:

    78 R8 C8CO9CRETE t.pe of fr"it

    (apple3

    oran%e3 an'

    >atermelon

    )

    T/e ol"me of

    fr"it "i,e t/at

    'e,olo"rie' t/e

    ;CPIP ol"tion

    8ol"me,on,ent

    ration of ;CPIP

    ABSTRACT 0 per,enta%e of

    itamin C

    7ETHO;S

    Oater

    ($MC)3

    /Con,entrationof ,aron

    'io&i'e

    ABSTR ACT li%/t

    intenit.

    Rate of

    p/oto.nt/ei

    0

    7ETHO;S

    Oit/ a /arp

    raor la'e "n'er >ater- "K1'"K)

    #- T/e tran' of"ydrilla p- i pla,e' ini'

    a %la filter f"nnel."K1'

    +- T/e f"nnel i pla,e' "pi'e 'o>n in a +55ml ea6er."K1'

    ?- T/e ea6er i fille' >it/ #55 ml of 14

    o'i"m i,aronate."K2'

    @- T/e ea6er i pla,e' at a 'itan,e of +5 ,m

    from t/e ?5 "l a a li%/t o"r,e ."K3'- T/e n"mer of %a "le releae' in on

    min"te are ,o"nte' an' re,or'e' in a tale

    "K4'- T/i tep i repeate' t>i,e ."K'- Step @ i repeate' . pla,in% t/e apparat" a

    'itan,e #5 ,m3 *5 ,m3 $5 ,m an' 15 ,mfrom t/e li%/t o"r,e."K3'

    15-T/e re"lt are re,or'e' in a tale"K1'11-T/e %rap/ of t/e rate of p/oto.nt/ei

    a%aint t/e li%/t o"r,e i plotte'- "K1'

    R-/:;itan,e of

    li%/t

    o"r,e-

    (,m)

    9o of %a

    "le

    releae'

    T/e rate of

    p/oto.nt/e

    i

    (min01)

    +5

    #5

    *5

    $5

    15

    ? ?-11 IN(ESTIGATING THEEFFECT OF CARBONDIOXIDE CONCENTRATION

    ON THE RATE OF

    PHOTOSYNTHESIS

    AIM:To t"'. t/e effe,t of ,aron 'io&i'e

    ,on,entration on t/e rate of

    p/oto.nt/ei-

    PROBLEM STATEMENT:

    ;oe of ,aron 'io&i'e ,on,entration

    affe,t t/e rate of p/oto.nt/ei

    HYPOTHESIS:A/en t/e ,on,entration of ,aron

    'io&i'e in,reae t/e rate of

    p/oto.nt/ei alo in,reae "ntil t/e

    rate e,ome ,ontant

    MATERIALS&APPARATUS:"ydrillaplant3 'ifferent ,on,entration of

    o'i"m /.'ro%en i,aronate (5-$435-#43 5-?4 an' 14)3 plati,ine3

    ?5 ele,tri, "l3 +55 ml ea6er3 a

    oilin% t"e3 top >at,/3 raor la'e3

    t/ermometer3 meter r"ler

    DIAGRAM:

    78 R8 C8CO9CRETE Con,entrati

    on of

    ,aron

    'io&i'e

    9"mer of %a

    "le t/at are

    releae in 1

    min"te-

    Temperat"re of

    t/e >ater

    ($MC)3 /Li%/tintenit.

    ABSTRACT 0 rate of

    p/oto.nt/ei

    0

    7ETHO;S

    O/i,/ are

    5-$43

    5-#43 5-?4

    an' 14

    )R("TECHNI

    UE'Count and

    record t/en"mer of %a

    "le t/at are

    releae in 1

    min"te . "in%

    a stopwatch.

    Fixed t/e

    'itan,e of

    "ydllra p- to

    o"r,e of li%/tto 15 ,m .

    "in% t/e r"ler-

    *R("TECHNI

    UE'calculate and

    record the rate

    of

    p/oto.nt/ei

    . "in% t/e

    form"lae:

    no- of "le

    releae'

    time(min01)

    K10 Ho> to et "p t/e apparat" C/ooe 15 ,m len%t/ of fre

    "ydrilla sp.

    T/e tran' of "ydrilla p- i

    pla,e' ini'e a oilin% t"e

    Clip t/e tip >it/ a paper ,lip an' p"

    it in t/e oilin% >it/ t/e ,lip 'o>n

    T/e %rap/ of t/e rate o

    p/oto.nt/ei a%aint t/e ,aro

    'io&i'e ,on,entration i plotte'

    K$0 Ho> to operate t/e ,ontant ariale

    Po"r #5 ml of 14 o'i"m

    i,aronate ol"tion into t/e oilin%

    t"e-

    Pla,e t/e apparat" at a fi& 'itan

    from a li%/t o"r,e >/i,/ i 15 ,m

    K# Ho> to operate t/e repon'in% ariale

    Co"nt an' re,or' t/e n"mer o

    "le releae' in + min"te T/e rate of p/oto.nt/ei i

    ,al,"late' . "in% t/e form"lae:

    (n"mer of "letime)

    K* Ho> to operate t/e manip"late

    ariale

    Repeat K1 to K# "in% 'ifferen

    per,enta%e of o'i"m i,aronat

    ol"tion >/i,/ are 5-$43 5-#4

    5-?4

    K+ 0 Pre,a"tion

    Pla,e t/e oilin% t"e in a ea6er o

    >ater to maintain t/e temperat"re

    R-/:Con,entrati

    on of

    9o of %a

    "le

    T/e rate of

    p/oto.nt/e

  • 7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc

    7/14

    ,aron

    'io&i'e (4)

    releae' in

    one min"te

    i (min01)

    1-5

    5-?

    5-#

    5-$

    @ @-1 STUDYING THE PROCESS OFAEROBIC RESPIRATION

    AIM:To t"'. t/e pro,e of aeroi,

    repiration

    HYPOTHESIS:T/e aeroi, repiration pro'",e ,aron

    'io&i'e to in,reae t/e /ei%/t of

    ,olo"re' liN"i'-

    MATERIALS&APPARATUS:ater3 ,olo"re' liN"i'3 a ,o,6roa,/ an'

    o'a lime3 oilin% t"e3 +55ml ea6er3

    $+5ml ea6er3 ,apillar. t"e3 ,re>

    ,lip3 an' a >ire %a"e-

    DIAGRAM:

    78 R8 C8CO9CRETE Preen,e of

    ,o,6roa,/

    Hei%/t of

    ,olo"re' liN"i'-

    Temperat"re of

    t/e >ater at/

    (*@MC)3 amo"nt

    of o'a lime-

    ABSTRACT 0 Pro,e of

    aeroi,

    repiration

    0

    7ETHO;S

    Oater at/

    >/i,/ i *@MC

    . "in% a

    t/ermometer

    *R("TECHNI

    UE'

    1- Lael t>o oilin% t"e >it/ A an' B(K1)

    $- ater an'

    temperat"re

    Temperat"re of

    t/e >ater at/

    (*@MC)3 ol"me

    of %l",oe

    ol"tion( 1+ ml)38ol"me of lime

    >ater($ml)

    ABSTRACT 0 pro,e of

    aeroi,repiration

    0

    7ETHO;S

    Oater at/

    >/i,/ i *@MC

    . "in% a

    t/ermometer

    */- "C'

    L7

    o oilin% t"e >it/ A an' B(K1)

    *-

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    8/14

    @ @-$"SLG

    RJ #'

    IN(ESTIGATING THE

    DIFFERENCES BET?EEN

    THE INHALED AND

    EXHALED AIR IN TERMS OF

    OXYGEN AND CARBON

    DIOXIDE CONTENTS

    AIM:To 'etermine t/e o&.%en an' ,aron

    'io&i'e ,ontent in in/ale' an' e&/ale'

    air

    PROBLEM STATEMENT:

    ;oe in/ale' air ,ontain more o&.%enan' le ,aron 'io&i'e t/an e&/ale'

    air

    HYPOTHESIS:I8*6 *,ontain more o&.%en *6

    le ,aron 'io&i'e t/an e&/ale' air

    I8*6 *,ontain more ,aron

    'io&i'e *6le o&.%en t/an e&/ale' air

    E8*6 *,ontain more ,aron

    'io&i'e *6le o&.%en t/an in/ale' air

    0E8*6 *,ontain more o&.%en *6le ,aron 'io&i'e t/an in/ale' air-

    MATERIALS&APPARATUS:

    Potai"m /.'ro&i'e ol"tion3

    Potai"m p.ro%allate ol"tion

    3ater3 0t"e3 R"ler3 Bea6er 3 Boilin%

    t"e 3 Bain >ater at/3 R"er t"in%

    DIAGRAM:

    78 R8 C8CO9CRETE 0t/e len%t/ of air

    ,ol"mn o,,"pie'

    . o&.%en in

    in/ale'e&/ale'

    air

    0 t/e len%t/ of

    air ,ol"mn

    o,,"pie' .

    ,aron 'io&i'e

    in

    in/ale'e&/ale'

    air

    0T8 /*

    ;/8 5 *

    )-7(Same)

    t"'ent

    0t"e

    ;iameter of 0

    t"eCon,entrat

    ion of KOH

    Temperat"re

    Time to ,olle,t

    air ampleAir

    ampleABSTRACT In/ale' air

    an' e&/ale'

    air

    per,enta%eN"ant

    it. of o&.%en

    an' ,aron

    'io&i'e iin/ale'

    an' e&/ale' air

    0

    7ETHO;S

    Oa ,/oen a t/e fiel' t"'.-

    "K1'

    $- F"a'rat of ie 1m & 1m >a "e'-

    "K2'"K1'

    *- T>o plant pe,ie pe,ie A an' B >a

    i'entifie'."K1' "K3'#- T/e N"a'rat >ere t/ro>n at ran'om in t/e

    ,/ool fiel' "K1'+- T/e area of (,oera%e) ea,/ plant pe,ie

    pe,ie A an' pe,ie B >a ,o"nte'- "K4'?- T/e n"mer of in'ii'"al plant pe,ie in

    ea,/ N"a'rat >a ,o"nte'-"K4'@- If more t/an /alf of t/e N"are in t/e

    N"a'rat i ,oere'3 t/e area of plant pe,ie

    P)/*; 5 O2 ! _(y z)_cm 1##=

    x cm

    & len%t/ of air ,ol"mn of in/ale'e&/ale' air

    . len%t/ of air ,ol"mn "pon a''in% porai"m

    P)/*; 5 CO2 ! _(x y)_cm 1##=

    x cm

    & len%t/ of air ,ol"mn of in/ale' e&/ale' air

  • 7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc

    9/14

    pe,ie A an' B in t/e ,/ool fiel'

    $ ;oe t/e t.pe of plant pe,ie affe,t

    t/e per,enta%e ,oera%e pop"lation

    ie 'enit. of t/e plant

    *- /i,/ t.pe of t/e plant pe,ie

    pe,ie A or B /a t/e /i%/et

    per,enta%e ,oera%e pop"lation ie

    HYPOTHESIS:1- T/e per,enta%e ,oera%e

    pop"lation ie of pe,ie A plant i

    /i%/er t/an pe,ie B in t/e ,/ool

    fiel'-$- ;ifferent plant pe,ie /ae 'ifferent

    per,enta%e ,oera%e pop"lation ie -

    *- Plant pe,ie A i more 'ominant

    t/an pe,ie B in t/i /aitat-

    MATERIALS&APPARATUSPlant pe,ie A an' B an. $ plant

    peie Plati, N"a'rat3 mar6er pen3 A#

    Paper3 %rap/ paper-

    DIAGRAM:

    plant

    ;enit. of

    pe,ie

    7ETHO;S

    O/i,/ are

    pe,ie A

    an' pe,ie

    B

    )R("TECHNI

    UE'

    Measure and

    recordt/e area

    of ea,/ t.pe of

    pe,ie "in% a

    N"a'rat 1m & 1m

    Fixed t/e ie of

    N"a'rat to

    1m1m

    *R("TECHNIUE'

    6efer to the

    sentences below.

    M/86 *R(Calculate and record t/e per,enta%e ,oera%e of plant

    pe,ie A an' B "in% the formulae

    Total area ,oere' . t/e pe,ie Q 1554

    9"mer of N"a'rat Q area of one N"a'rat

    Cal,"late t/e 'enit. of plant pe,ie "in% t/e form"la:

    Total n"mer of or%anim in all N"a'rat

    9"mer of N"a'rat Q area of one N"a'rat

    R-/

    >ill e ,o"nte' - T/e area i not ,o"nte' if

    onl. le t/an /alf i ,oere'-"K'

    - Step + to @ >a repeate' for nine N"a'rat-

    "K1'

    - T/e area ,oere' . plant pe,ie pe,ie A

    an' pe,ie B n"mer of in'ii'"al plant

    pe,ie t"'ie' in ea,/ N"a'rat >erere,or'e' an' ta"late' in a tale- "K4'

    15- T/e per,enta%e ,oera%e 'enit.

    freN"en,. of plant pe,ie pe,ie A an'

    pe,ie B >ere ,al,"late' . "in% t/i

    form"la: "K4'

    11- P)/*; )*; 5 >*/ >) :

    Total area ,oere' plant pe,ie in all

    N"a'rat Q 1554

    Total n"mer of N"a'rat Q area of a N"a'rat

    F-) 5 >)

    9"mer of N"a'rat ,ontainin% plant pe,ie Q

    1554

    Total n"mer of N"a'rat

    1$- D/ 5 >*/ >)

    total n"mer of in'ii'"al pe,ie in all

    N"a'rat

    Total n"mer of N"a'rat Q area of a N"a'rat

    -? ESTIMATING THEPOPULATION SIE OF

    GARDEN SNAILS USING

    CAPTURE, MARK, RELEASE

    AND RECAPTURE

    TECHNIUE

    AIM:To etimate t/e pop"lation ie of

    %ar'en nail "in% ,apt"re3 mar63

    releae an' re,apt"re te,/niN"e-

    PROBLEM STATEMENT:

    /at i t/e effe,t of 'ifferent ie of

    ample on t/e ie of %ar'en nailpop"lation

    HYPOTHESIS:T/e lar%er t/e ie of ample3 t/e more

    a,,"rate of t/e nail pop"lation

    etimate'-

    MATERIALS&APPARATUSSnail3a paintr"/3a ottle of In'ian

    in63 a pen an' a noteoo6-

    DIAGRAM:

    78 R8 C8CO9CRETE T/e ie of

    ample

    0 T/e ie of

    reear,/ area

    ABSTRACT 0 t/e %ar'en nail

    pop"lation

    0

    7ETHO;S

    O/i,/ are

    mall an'

    lar%e

    ample

    )R("TECHNI

    UE'

    0

    Fixed t/e ie of

    reear,/ area to

    1+m1+m

    *R("TECHNI

    UE'calculate and

    record t/e t/e

    %ar'en nailpop"lation .

    "in% t/e

    form"lae:

    Pop"lation ie

    % a - b& 7 c

    a T/e n"mer

    of nail

    in t/e firt

    ,apt"re

    n"mer of

    nail

    in t/e e,on',apt"re

    , no of mar6e'

    ir' in e,on'

    ,apt"re-

    R-/

    9"mer of nail

    Sie of %ar'en nail

    pop"lation

    Sample of

    firt ,apt"re

    Se,on' ,apt"re

    Total

    n"mer

    7ar6e'

    nail

    1t

    ample

    1*- Sele,t a "itale lo,ation >it/in .o"r ,/ool

    ,ompo"n' >it/ t/e area 1+m1+m ."K1'

    "K2'1#- Capt"re a man. %ar'en nail a .o" ,an

    from t/e pla,e-"K1'

    1+- Co"nt t/e %ar'en nail t/at .o" /ae

    ,apt"re' an' mar6 t/eir /ell >it/ a mall

    'ot of In'ian in6- "K4'"K'1?- Releae t/em in t/e ame pla,e >/ere .o"

    fo"n' t/em "K1'1@- Go a,6 to t/e ame pla,e after een 'a.-

    Capt"re on,e a%ain a man. %ar'en nail a

    .o" ,an-

    1- Co"nt t/e total n"mer of %ar'en nail .o"

    /ae ,apt"re' an' note t/e n"mer of t/oe>/i,/ /a' een mar6e'."K4'

    1- Re,or' t/e 'ata in a tale "K1'

    $5- Calculate and record t/e pop"lation ie of

    %ar'en nail "in% t/e follo>in% form"la:

    Pop"lation ie % a - b& 7 c

    a8 ,he number of snailsin the first capture

    b8 number of snails

    in the second capture

    c8 no of mar9ed snails in second

    capture1*- Repeat t/e tep $ to . "in% t/e lar%er

    n"mer of nail ample-"K3'

    1#- Re,or' t/e 'ata in t/e tale- "K1'

  • 7/25/2019 CHECKLIST PAPER 3 BIOLOGY.doc

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    $n'

    ample

    -@"SBP

    #0'

    IN(ESTIGATING THE

    EFFECT OF A CHANGE IN PH

    "ABIOTIC FACTOR' ON THE

    POPULATION GRO?TH

    RATE OF AN ORGANISMAIM:To ineti%ate t/e effe,t of ,/an%e in pH

    al"e on t/e pop"lation %ro>t/ rate of

    :emnap- plant-

    PROBLEM STATEMENT:

    1- ;oe t/e ,/an%e in pH al"e affe,t

    t/e pop"lation %ro>t/ rate of

    :emnap plant

    $- /at i t/e effe,t of ,/an%e in pH

    al"e on t/e pop"lation %ro>t/ rate

    of:emnap-

    *- /i,/ pH al"e i t/e mot "itale

    for t/e in,reae in pop"lation of

    :emnap-

    HYPOTHESIS:1- T/e pH @ i t/e mot "itale for t/e

    in,reae in pop"lation of:emnap-

    plant ,ompare' to pH $ an' pH 1#-$- T/e pop"lation %ro>t/ rate of

    :emnap- plant i t/e /i%/et in

    t/e pH @ ,ompare' to pH $ an' pH

    1#-

    MATERIALS&APPARATUS:

    :emnap- plant3 D'itille' >ater 'il"te

    /.'ro,/lori, a,i' o'i"m /.'ro&i'e

    ol"tion3 ,"lt"re ol"tion pon' >ater-

    Bea6er petri 'i/ ,ontainer3

    mea"rin% ,.lin'er3 pH paper meter-

    DIAGRAM:

    78 R8 C8CO9CRETE T.pe o f

    ol"tion

    "e'

    T/e in,reae in

    pop"lation of

    :emnap-

    plant-

    Spe,ie of

    :emnap-

    plant ol"me

    of >ater ,"lt"reol"tion

    ,on,entration of

    n"trient

    temperat"re

    li%/t intenit.

    time

    ABSTRACT ;ifferent

    pH al"e

    pH $3 pH @

    an' pH 1#

    T/e pop"lation

    %ro>t/ rate of

    :emnap-

    plant-

    0

    7ETHO;S

    Ot/ rate of

    :emnap-

    plant ('a.01)

    ;a.01 ;a.0+In,reae

    ;e,reae

    9e"tral

    A,i'i,

    Al6ali

    1- C/ooe:emna p- plant of t/e ame ie-

    "K1'"K2'$- C/ooe ta6e t/ree petri 'i/e of t/e am

    ie."K1'

    *- Lael t/e petri 'i/e a A3 B an' C."K1'#- Po"r + ml of 'itille' >ater into petri 'i/

    A3 + ml of /.'ro,/lori, a,i' into petri 'i/

    B an' + ml of o'i"m /.'ro&i'e ol"tion

    into petri 'i/ C- "K1'"K3'

    +- Tet t/e pH al"e of ea,/ ol"tion "in% pH

    paper (an' re,or' in a tale)-"K1'

    ?- Po"r + ml of ,"lt"re ol"tion pon' >ater

    into ea,/ petri 'i/-"K1'"K2'

    @- P"t +:emna sp.plant into ea,/ petri 'i/-

    "K1'

    - Re,or' in a tale."K1'- Pla,e t/e petri 'i/e on t/e tale near t/e

    >in'o> in t/e laorator.."K2'15-C/an%e t/e ol"tion in t/e petri 'i/e eer.

    'a.-"K'11-Co"nt t/e n"mer of:emna sp- plant after

    + 'a.-"K4'1$-Cal,"late t/e pop"lation %ro>t/ rate of

    :emna sp.plantusing a formula "K4'

    T/e pop"lation %ro>t/ rate of Lemna p-

    9"mer of:emna sp + 'a.

    1*-Repeat t/e e&periment tep 1 "ntil 11 to

    %et t/e a,,"rate re"lt- "K'

    14.T*9-*/ /8 6*/* /8 /*9 9at,/3 >ater at/3 t/ermometer-

    DIAGRAM:

    78 R8 C8

    CO9CRETE ;ifferenttemperat"re

    of >ater

    at/

    Hei%/t of t/e,olo"re' liN"i'

    in t/e

    manometer

    8ol"me of .eat"penion

    ABSTRACT 0 0

    7ETHO;S

    Oater poll"tion

    at lo,ation P F R

    HYPOTHESIS:T/e >ater at lo,ation R i more poll"te'

    ,ompare' to lo,ation P an' lo,ation F-

    T/e >ater at lo,ation R i t/e motpoll"te' ,ompare' to lo,ation P an'

    lo,ation F T/e met/.lene l"e ol"tion

    too6 t/e /ortet time to 'e,olo"rie in

    ample >ater R ,ompare' to ample

    >ater F an' P-

    MATERIALS&APPARATUS:7et/.lene l"e ol"tion (5-14)3 >ater

    ample3 top >at,/3 rea%ent ottle3

    .rin%e >it/ nee'le3 mea"rin% ,.lin'er-

    DIAGRAM:

    78 R8 C8CO9CRETE Lo,ation of

    >ater

    Time ta6en to

    'e,olo"rie

    met/.lene l"e

    ol"tion

    8ol"me of >ater

    ample

    (155ml)

    ol"me of

    met/.lene l"e

    ol"tion (1ml)

    ,on,entration of

    met/.lene l"eol"tion

    ABSTRACT 0 Leel of >aterpoll"tion 0

    7ETHO;S

    Oater

    ample

    from t/e

    lo,ation P3

    F3 an' R

    )R("TECHNI

    UE'Measure and

    record time to

    'e,olo"rie

    met/.lene l"e

    ol"tion . "in%

    a stopwatch

    Fixed t/e

    ol"me of

    met/.lene l"e

    ol"tion >/i,/

    i 1 ml by using

    a measuring

    cylinder.

    *R("TECHNI

    UE'*

    R-/:

    Rea%e

    ntottle

    ater

    ample

    Time ta6en to

    'e,olo"riemet/.lene

    l"e ol"tion (/)

    Leel of >ater

    poll"tion(/01)

    A P

    B F

    C R

    1- ater ample are ,olle,te' from P3F an' R

    "K1'$- T/e rea%ent ottle are laelle' A3B3C- "K1'

    *- 7ea"re 1## 7 5 from P3F

    an' R eparatel. an' po"r into t/e rea%ent

    ottle laelle' A3B an' C repe,tiel.- "K2'

    "K3'#- 1 7 5 7/8 9- -/i a''e' to

    t/e ae of ea,/ >ater ample "in% a

    .rin%e-"K2'

    +- T/e rea%ent ottle are ,loe' >it/ t/etopper imme'iatel.."K1' "K'

    ?- T/e ,ontent of t/e ottle ,annot e /a6en

    "K'@- All t/e rea%ent ottle are 6ept in a 'ar6

    ,"poar' "K'- T/e top>at,/ i a,tiate'."K1'

    - T/e ottle are e&amine' from time to time.

    "K1'

    15-T/e time ta6en for t/e met/.lene l"e

    ol"tion to 'e,olo"rie e,ome ,olo"rle

    i re,or'e' for all t/e >ater ample."K4'11-T/e re"lt are re,or'e' in a tale- "K1'

    15 15-@ SHO?ING XYLEM AS ACONTINUOUS TUBE SYSTEM

    THAT TRANSPORTS ?ATER

    AND MINERALS

    AIM:To /o> t/at &.lem a a ,ontin"o" t"e

    ,an tranport >ater an' mineral-

    PROBLEM STATEMENT:;oe Q.lem ti"e form a ,ontin"o"

    t"e .tem t/at tranport >ater an'

    mineral from t/e root to t/e /ootHYPOTHESIS:Q.lem ti"e form a ,ontin"o" t"e

    .tem t/at tranport >ater an'

    mineral from t/e root to t/e /oot-

    Q.lem ti"e ,an tranport >ater an'

    mineral-

    MATERIALS&APPARATUS:A alam plant3 eoin ol"tion3 ea6er3

    raor la'e3 mi,ro,ope li'e3 ,oer

    lip3 a mi,ro,ope3 for,ep3 a >/ite tile3

    petri 'i/ an' a paintr"/-

    DIAGRAM:

    78 R8 C8CO9CRETE Part of

    plant at

    leae3 tem

    an' root

    T/e ti"e

    taine' re' >it/

    t/e re' ,olor of

    eoin-

    T.pe of plant

    ABSTRACT 0 Part of plant

    ti"e t/at ,an

    tranport >ater

    an' mineral-

    0

    7ETHO;S

    O/i,/

    are at leaf3

    tem an'

    root

    )R("TECHNI

    UE'

    Observe and

    record t/e

    ti"e taine'

    re' >it/ t/e re'

    ,olor of eoin .

    "in% a

    microscope.

    Used the same

    t.pe of plant

    >/i,/ i t/e

    Balam plant-

    *R("TECHNI

    UE'*

    1- T/e root of Balam plant i >a/e' "K1'

    "K2'$- T/e root i immere' in eoine ol"tion for

    *5 min"te- (K1)

    *- /en t/e re' eoin ol"tion /a penetrate'

    into t/e ein of t/e leae3 t/e plant i

    remoe'- (K+)

    #- T/in e,tion of t/e tem are ,"t >it/ raor

    la'e-(K1)

    +- A paintr"/ i "e' to tranfer a t/in ,ro

    e,tion of t/e tem onto a 'rop of >ater on a%la li'e-(K1)

    ?- T/e e,tion i ,oere' >it/ a ,oer lip an'

    e&amine' "n'er a mi,ro,ope-(K1)

    @- T/e ti"e >/i,/ /a een taine' >it/ t/e

    re' ,olo"r of eoin i i'entifie'(K#)

    - Step #0? are repeate' >it/ t/e ,ro e,tion

    of root an' leaf-(K*)

    - ;ia%ram of ,ro e,tion of t/e root3 tem

    an' leaf "n'er lo> po>er i 'ra>n an'in'i,ate' t/e part taine' re'-(K#)

    R-/:Part of plant Cro e,tion of

    plant

    Leaf

    Stem

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    Root

    15 15-* CARRYING OUT BARKRINGING TO SHO? THE

    ROLE OF PHLOEM IN THE

    CONTINUOUS TRANSPORT

    OF ORGANIC SUBSTANCES.

    AIM:

    To /o> t/e role of p/loem in t/e,ontin"o" tranport of or%ani,

    "tan,e-

    PROBLEM STATEMENT:

    1-/at i t/e effe,t of remoin% a rin%

    of p/loem ti"e from t/e tem of a tree

    $- ;oe t/e remoin% a rin% of p/loemti"e from t/e tem of a tree >ill affe,t

    t/e tranportation of or%ani, "tan,e

    HYPOTHESIS:T/e rin%e' tem /o> ti"e aoe t/e

    rin% >ell3 >/erea t/e ti"e elo>

    t/e rin% ten' to >it/er-

    MATERIALS&APPARATUS:

    S/arp 6nife3 Healt/. /ii,"

    tree38aelin an' mea"rin% tape-

    DIAGRAM:

    78 R8 C8CO9CRETE A tem t/at

    i rin%e'

    an' a tem

    t/at i not

    rin%e'

    T/e ,on'ition

    aoe an' elo>

    t/e rin% after one

    mont/

    T/e 'iameter

    of t/e temaoe an' elo>

    t/e rin% after one

    mont/-

    Con'ition of

    enironment3

    t.pe of plant3

    time of t/e

    e&periment

    ABSTRACT 0 Part of plant

    ti"e t/at ,an

    tranport or%ani,

    "tan,e-

    0

    7ETHO;S

    O t/e rin%

    after one mont/

    . "in%ameasuring tape.

    Used the same

    t.pe of plant>/i,/ i t/e

    Hii," plant-

    *R("TECHNI

    UE'*

    1- T>o tree tem of /ii," plant are ,/ooen

    an' laelle' A an' B-(K1) (K$)

    $- A 6nife i "e' to remoe a ,omplete rin% o

    ar6 from a tree tem A- (K1)

    *- 8aeline i applie' on t/e e&poe' ti"e-

    (K1)(K+)

    #- ;ra> t/e ,on'ition of tem -Measure andrecord initial 'iameter of t/e tem aoe

    an' elo> t/e rin% . "in% a measuring

    tape.(K#)(K1)

    +- After 7/83 t/e ,on'ition of t/e rin%e'

    tem aoe an' elo> t/e rin% are oere'

    an' re,or'e'-( K#)(K$)

    ?- A 'ra>in% of t/e tem ,on'ition i

    'ra>n-Measure and record t/e 'iameter of

    t/e tem aoe an' elo> t/e rin% after one

    mont/ . "in% a measuring tape.(K#)

    @- Repeat tep #3 + an' ? . "in% tem B for

    not rin%e' tem-(K*)

    - T/e ,on'ition of t/e tem i ,ompare' to t/e

    tem t/at i not rin%e'-(K#)

    R-/:T.pe of

    tem

    ;iameter

    (,m)

    ,on'ition

    Before one

    mont/

    ;iameter

    (,m)

    ,on'ition

    After one

    mont/

    A Rin%e'

    Stem

    B 9ot

    Rin%e'

    tem

    15 15- STUDYING THE EFFECT OFAIR MO(EMENT ON THE

    RATE OF TRANSPIRATION

    BY USING POTOMETER.

    AIM:To t"'. t/e effe,t of air moement on

    t/e rate of tranpiration-

    PROBLEM STATEMENT:

    /at are t/e effe,t of t/e tate 'ifferentpee' of air moement on t/e rate of

    tranpiration

    HYPOTHESIS:A t/e pee' of t/e air moement

    in,reae3 t/e rate of tranpiration

    in,reae

    T/e rate of tranpiration i /i%/er in a

    moin% air t/an in a till air

    MATERIALS&APPARATUS:Capillar. t"e3 r"er t"e3 top >at,/3

    r"ler3 ea6er3 fan3 retort tan' >it/

    ,lamp3 raor la'e3 ain3 mar6er3 Plant

    /oot3 >ater3 aeline

    DIAGRAM:

    78 R8 C8CO9CRETE State

    pee' of air

    moement

    Time ta6en for

    air "le to

    trael from P to

    F (min)

    Con'ition of

    enironment3

    t.pe of plant3

    ABSTRACT 0 Rate of

    tranpiration

    0

    7ETHO;S

    O/i,/ i t/e

    Hii," plant-

    / Fixedt/e

    temperat"re of

    "rro"n'in% to

    *@MC . using

    the

    thermometer.

    *R("TECHNI

    UE'Calculate and

    record t/e rate of

    tranpiration .

    "in% t/e

    form"lae of

    distance

    travelled dividedby time ta9en

    R-/:

    State of

    air

    moem

    entSpe

    e' of

    t/e fan

    Time ta6en for air "le to trael from Q

    to Y3 (min)

    Rate of

    tranpir

    ation

    min01

    1 1 $ * Aera%e

    $

    1. C8 *6 )-/ 55 * *5 8/ 57 *

    89)- >*/."K1'"K2'

    2. I77 /8 )-/ 6 776*/ / *

    9* 5 it/ t/e ot/er en' of t/e ,apillar.

    t"e immere in a ea6er of >ater (K1)

    $. ipe 'r. t/e leae an' apparat" "in% 'r.

    ,lot/

    0. Smear all t/e oint of t/e apparat" >it/

    aeline to preent lea6a%e (K+)

    1#.Pla,e t/e apparat" "n'er t/e fan >/i,/ i

    >it,/e' off-

    11.Intro'",e an air "le into t/e ,apillar.

    t"e . liftin% t/e en' of t/e ,apillar. t"eo"t of t/e ea6er for a /ort >/i le an' t/en

    ret"rne' it to t/e ea6er a%ain (K1)

    12.Allo> t/e air "le to moe "ntil it rea,/et/e P mar6 an' a,tiate t/e top >at,/

    13.Stop t/e top >at,/ >/en t/e air "le

    rea,/e t/e F mar6

    14.Measure and record the time ta6en for air

    "le to trael from P to Fby using a

    stopwatchin t/e tale proi'e' (K#) (K1)

    1.Repeat tep 1to 1# to otain an aera%e

    rea'in%

    1.Repeat t/e aoe tep . >it,/in% on t/e

    fan "in% 'ifferent pee' of t/e fan (pee'

    13 $3 *3 #3 +) an' re,or' t/e re"lt (K*)

    K1 : P>**/ 5 >)7 *6 *>>**/- %

    >/7/ "*/ */ 4S / ;/ * /)@'

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    13/14

    *

    #

    +

    S1: C/ooe a leaf. /oot

    S$: C"t t/e tem oliN"el.

    S*: Inert t/e r"er t"e into ,apillar. t"e

    S#: Inert t/e ,"t tem to t/e r"er t"e

    S+: Set "p t/e apparat" in "pri%/t poition

    S?: Intro'",e t/e air "le

    K3 : H*6; /8 7*>-*/6 **9

    it,/ on an' fan >it,/ on

    K2 : H*6; /8 )/6 **9

    C/ooe an' ,"t off a leaf. /oot from a /ii,"

    plant-

    K4 : H*6; >6; **9 %C)/; *6 )6; 6*/*

    0 a,tiate t/e top >at,/ at Q

    0 top t/e top >at,/ at Y

    *Measure and record the time ta6en for air

    "le to trael from P to Fby using a

    stopwatch

    K : A))-*) 5 /8 6*/* 9/*6 %

    P)*-/0 repeat t/e tep an' fin' t/e aera%e

    0 mear >it/ aeline to preent lea6a%e >ipe

    'r. t/e leae an' apparat"

    1$ 1$-1 STUDYING THE EFFECT OFDIFFERENT UANTITIES OF

    ?ATER INTAKE TO THE

    URINE OUTPUT

    AIM:

    To t"'. t/e effe,t of 'ifferent N"antitieof >ater inta6e ol"me of 'rin6 on t/e

    ol"me of "rine o"tp"t-

    PROBLEM STATEMENT:

    /at i t/e effe,t of 'ifferent N"antitie

    of >ater inta6e on t/e ol"me of "rine

    o"tp"t

    HYPOTHESIS:T/e more >ater (78) 'r"n63 t/e more

    t/e ol"me of "rine o"tp"t (R8) i

    forme'-

    T/e /i%/er t/e N"antit. of >ater inta6e

    (78)3 t/e more t/e ol"me of "rine

    o"tp"t (R8) i forme'-

    MATERIALS&APPARATUS:St"'ent3 paper ,"p3 'rin6in% >ater3

    mea"rin% ,.lin'er-

    DIAGRAM:

    78 R8 C8CO9CRETE 8ol"me of

    'rin6

    F"antit. of

    >ater

    inta6e .t"'ent-

    8ol"me of "rine

    pro'",e

    Enironmental

    ,on'ition

    (temperat"re3

    /"mi'it.)

    Gen'er3 ie an'a%e of t"'ent

    ABSTRACT 0

    7ETHO;S

    Oater3

    t"'ent B *55 ml of mineral >ater3 t"'ent C

    #55 ml of mineral >ater an' t"'ent ; +55

    ml of mineral >ater to 'rin6-(K1)(K*)

    #- Measure and recordt/e ol"me of "rine

    o"tp"t of t/e t"'ent >it/in t/at /o"r-(K#)

    +- T/e "rine pro'",e' i ,olle,te' in paper

    ,"p an' mea"re' into mea"rin% ,.lin'er

    (K1)

    ?- Re,or' t/e ol"me of "rine pro'",e' .

    ea,/ t"'ent in tale-(K1)

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    1+ 1+-1 IN(ESTIGATING (ARIATIONIN HUMANS

    AIM:To ineti%ate t/e t.pe of

    ariation(78) amon% t"'ent(R8) in +

    a"/ari-

    To t"'. t/e n"mer of t"'ent(R8)

    >it/ 'ifferent /ei%/t an' t.pe of

    fin%erprint(78)

    PROBLEM STATEMENT:1- ;o all t/e t"'ent /ae t/e ame

    t.pe of fin%erprint an' /ei%/t t.pe

    of ariation$- ;o 'ifferent t.pe of fin%erprint an'

    /ei%/t affe,t t/e n"mer of t"'ent

    HYPOTHESIS:;ifferent -79 5 /-6/(R8)

    /o> 655/ /> 5 5;>/

    *6 8;8/ "M(' % /> 5 **/

    inerel.

    MATERIALS&APPARATUS:

    1' S/-6/ M

    $) Grap/ paper 0 7

    *) A# paper >/ite

    paper 0 7

    #) ti"e paper ,lot/ 0

    7

    +) /i,/are 1@ .ear ol'-

    *R("TECHNI

    UE'*

    R-/:

    St"'ent2

    name

    T.pe of fin%er print Hei%/t

    "7'>/orl C"re Compoite loop

    1-

    $-

    *-

    #-

    +-

    ?-

    @-

    -

    -

    15

    @- Ten name of t"'ent in t/ ame a%e >ere

    >ritten 'o>n in a tale (K1) (K$)

    - T/e /ei%/t i mea"re' . "in% a metre

    r"ler an' re,or'e' in a tale- (K1) (K#)

    - T/ e&periment i repeate' . ineti%atin%

    t/ t.pe of fin%erprint( K*)15-B. "in% a fin%erprint pa'3 pla,e' t/e

    t/"mprint on a >/ite papert>i,e- ( K1)

    11-B. "in% a /an' len3 t/ t.pe of t/"m prin

    >ere oere' an' i'entif.- (K#)

    1$-Step $ "ntil + >ere repeate' to ot/er

    t"'ent in t/ ame %ro"p- (K*)1*-T/ mea"rement of /ei%/t an' fin%erprint

    are repeate' t>i,e to %et t/ aera%e- (K+)

    1#-T>o %rap/ on t/ n"mer of t"'ent

    a%aint t/ t.pe of ariation >ere plotte'-

    (K1)