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![Page 1: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/1.jpg)
Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy
protein productionBy Xin Li
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![Page 2: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/2.jpg)
• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.
Purposes
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• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.
• To produce cheaper soydiesel, more soy protein, and less expensive ethanol from the soybean plants.
Purposes
![Page 4: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/4.jpg)
Modeling Soybean
Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
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Modeling Soybean
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Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
![Page 6: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/6.jpg)
Modeling Soybean
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are needed to see this picture.
Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
![Page 7: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/7.jpg)
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
![Page 8: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/8.jpg)
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
![Page 9: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/9.jpg)
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
•Insertion-induced Arabidopsis mutants are available commercially
![Page 10: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/10.jpg)
Insertion-Induced Mutation
T-DNA
Gene
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Insertion-Induced Mutation
![Page 12: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/12.jpg)
Insertion-Induced Mutation
![Page 13: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/13.jpg)
Insertion-Induced Mutation
![Page 14: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/14.jpg)
Insertion-Induced Mutation
![Page 15: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/15.jpg)
Insertion-Induced Mutation
![Page 16: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/16.jpg)
![Page 17: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/17.jpg)
Wild-type
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Hypersensitive mutant
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Insensitive mutant
![Page 20: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/20.jpg)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
![Page 21: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/21.jpg)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
![Page 22: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/22.jpg)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
![Page 23: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/23.jpg)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
• Measured root length of seedlings grown in glucose (Gibson, 2005)
![Page 24: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/24.jpg)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
• Measured root length of seedlings grown in glucose (Gibson, 2005)
• Use spectrophotometry to determine anthocyanin levels of seedlings grown in sucrose (Nacry,1998)
![Page 25: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/25.jpg)
Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for
SALK_113292 are representative of all other 57 Arabidopsis mutants tested
0.000.200.400.600.801.001.201.401.601.802.00
SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072
Col-O
Root lengths
Results
![Page 26: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/26.jpg)
Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for
SALK_113292 are representative of all other 57 Arabidopsis mutants tested
0.000.200.400.600.801.001.201.401.601.802.00
SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072
Col-O
Root lengths
Results
![Page 27: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/27.jpg)
Results
![Page 28: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/28.jpg)
Results
![Page 29: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/29.jpg)
ResultsNeff and Chory (1998)
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Results
![Page 31: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/31.jpg)
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
![Page 32: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/32.jpg)
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
![Page 33: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/33.jpg)
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is at1g06230.
![Page 34: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/34.jpg)
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is at1g06230.
• The gene contains a bromodomain protein
![Page 35: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/35.jpg)
Future Work
• Grow SALK_113292 in 1% glucose and 1% sucrose
![Page 36: Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production.](https://reader035.fdocuments.in/reader035/viewer/2022062308/56649ce15503460f949aba2e/html5/thumbnails/36.jpg)
Future Work
• Grow SALK_113292 in 1% glucose and 1% sucrose
• Grow SALK_113292 in 1% sorbitol and 6% sorbitol
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Image from: htto://www.food-info.net
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Acknowledgement
• Dr. Sue Gibson
• Dr. Chunyao Li
• Ms. Lois Fruen
• Ms. Chelen Johnson
• Dr. Jacob Miller
• Ms. Virginia Amundson
• Breck Advanced Team Research
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http://www.cbs.umn.edu/plantbio/faculty/GibsonSue/
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Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy
protein productionBy Xin Li
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.