Characterization of herbicide-resistant Eucalyptus plants expressing phosphinothricin...
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POSTER PRESENTATION Open Access Characterization of herbicide-resistant Eucalyptus plants expressing phosphinothricin acetyltransferase gene Esteban González 1* , Carla Gugliermoni 1 , Milton Galvão 1 , Maria Fagundes 1 , Maria Ferreira 1 , Guilherme Almeida 1 , Henrique Alves 1 , Jose Gonsalves 1 , Fernando Silva 2 , Sergio Bentivenha 2 , Shinitiro Oda 2 , Eduardo Mello 1 From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011 Herbicide resistant crops are commercially advantageous for efficient field productivity by enabling use of non- selective herbicides for weed management. Herbicide resistant trees could be used to improve productivity and reduce the costs of forest management through to the first and second years post tree establishment. We describe the introduction of the phosphinothricin acetyl- transferase (bar) gene, which confers resistance to the herbicide glufosinate, into Eucalyptus pellita (clone EP11) and hybrid Eucalyptus (E. grandis x E. dunni - clone SP1383) under the control of a constitutive pro- moter. The transgenic lines produced showed a copy number ranging from 1 to 3 copies by Southern blot analysis. Herbicide resistance of the transgenic clones was assessed in the greenhouse by application of 200g/L phosphinotricin at the level of 6.0 L/ha and 4.0 L/ha (commercial rate). The effect of the two herbicide treat- ments was tested on 4-week and 6-month old plants derived from several transgenic events from the two dif- ferent Eucalyptus clones. All 4-week old non-transgenic control plants, as well as whole shoots of 6-month old control plants were killed by the herbicide by fifteen days after application. The same herbicide treatment effectively killed all the weeds in field conditions. All the transgenic plants showed strong resistance to the herbi- cide in all treatments with no observed negative effects. The use of herbicide resistant trees may provide an additional means for improving the economic efficiency of plantation forest management. Author details 1 FuturaGene Brazil Ltda, Brazil. 2 Suzano Papel e Celulose, Brazil. Published: 13 September 2011 doi:10.1186/1753-6561-5-S7-P135 Cite this article as: González et al.: Characterization of herbicide- resistant Eucalyptus plants expressing phosphinothricin acetyltransferase gene. BMC Proceedings 2011 5(Suppl 7):P135. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 FuturaGene Brazil Ltda, Brazil Full list of author information is available at the end of the article González et al. BMC Proceedings 2011, 5(Suppl 7):P135 http://www.biomedcentral.com/1753-6561/5/S7/P135 © 2011 González et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Transcript of Characterization of herbicide-resistant Eucalyptus plants expressing phosphinothricin...
POSTER PRESENTATION Open Access
Characterization of herbicide-resistant Eucalyptusplants expressing phosphinothricinacetyltransferase geneEsteban González1*, Carla Gugliermoni1, Milton Galvão1, Maria Fagundes1, Maria Ferreira1, Guilherme Almeida1,Henrique Alves1, Jose Gonsalves1, Fernando Silva2, Sergio Bentivenha2, Shinitiro Oda2, Eduardo Mello1
From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and DeliveryArraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011
Herbicide resistant crops are commercially advantageousfor efficient field productivity by enabling use of non-selective herbicides for weed management. Herbicideresistant trees could be used to improve productivityand reduce the costs of forest management through tothe first and second years post tree establishment. Wedescribe the introduction of the phosphinothricin acetyl-transferase (bar) gene, which confers resistance to theherbicide glufosinate, into Eucalyptus pellita (cloneEP11) and hybrid Eucalyptus (E. grandis x E. dunni -clone SP1383) under the control of a constitutive pro-moter. The transgenic lines produced showed a copynumber ranging from 1 to 3 copies by Southern blotanalysis. Herbicide resistance of the transgenic cloneswas assessed in the greenhouse by application of 200g/Lphosphinotricin at the level of 6.0 L/ha and 4.0 L/ha(commercial rate). The effect of the two herbicide treat-ments was tested on 4-week and 6-month old plantsderived from several transgenic events from the two dif-ferent Eucalyptus clones. All 4-week old non-transgeniccontrol plants, as well as whole shoots of 6-month oldcontrol plants were killed by the herbicide by fifteendays after application. The same herbicide treatmenteffectively killed all the weeds in field conditions. All thetransgenic plants showed strong resistance to the herbi-cide in all treatments with no observed negative effects.The use of herbicide resistant trees may provide anadditional means for improving the economic efficiencyof plantation forest management.
Author details1FuturaGene Brazil Ltda, Brazil. 2Suzano Papel e Celulose, Brazil.
Published: 13 September 2011
doi:10.1186/1753-6561-5-S7-P135Cite this article as: González et al.: Characterization of herbicide-resistant Eucalyptus plants expressing phosphinothricinacetyltransferase gene. BMC Proceedings 2011 5(Suppl 7):P135.
Submit your next manuscript to BioMed Centraland take full advantage of:
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at www.biomedcentral.com/submit
* Correspondence: [email protected] Brazil Ltda, BrazilFull list of author information is available at the end of the article
González et al. BMC Proceedings 2011, 5(Suppl 7):P135http://www.biomedcentral.com/1753-6561/5/S7/P135
© 2011 González et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly cited.
