Characterization Facility - · PDF fileresolution TEM, tomography, diffraction, ......
1
Characterization Facility at Liquid Crystal Institute of Kent State University Introduction • The Liquid Crystal Institute (LCI) Characterization Facility, is an open access user facility serving the nearby academic and industrial communities and beyond. Activities Service • Serving 70+ academic groups (from Kent, Akron, Cleveland, Rootstown, Columbus, Toledo, Cincinnati, OH and out-of-state) and industrial partners (Sherwin Williams, Lubrizol, ExxonMobil, Shire, KDI, Fischione, etc.). Self - Use Training • Providing one-on-one and group training on most of the available instruments. • Popular training courses include basic AFM, basic SEM, basic TEM, cryo-TEM, TEM specimen preparation of soft-matter materials, confocal laser scanning microscope, and high speed camera. Other instruments include Zeta potential and particle size analyzer, FTIR spectrometer/microscope, rheometer, 2D polarimeter, chroma meter, Abbe refractometer, differential scanning calorimeter (DSC), profilometer, polarized optical microscope, etc. Teaching and outreaching • Integrating lectures and lab-demos on TEM/SEM/AFM into 2 graduate and 1 undergraduate classes. • Giving introductory/research seminars for academic institutions and local companies. • Lab tour and demo available to public. • Promoting microscopy and characterization techniques. • Working with academic societies/instrument manufacturers, and co-hosting meetings, workshops and demos. Fluid Dynamic Properties • Providing a comprehensive series of instruments and analytical tools to characterize the following aspects of hard- and soft-matter materials and devices. • The LCI Characterization Facility serves also as a center of hands-on training and education on advanced analytical techniques. Atomic/nano-/micro-scale structure (TEM, SEM, SPM, Optical) Thermal and Phase Properties Optical and Electro-optical Properties Surface Properties Mechanical and Rheological Properties … Labs and Instruments • Providing self-use, staff operation and collaboration for different users. The Transmission Electron Microscopy (TEM) Lab features a versatile 200 kV microscope (FEI Tecnai G2 F20, 0.24 nm point-to- point resolution). Available techniques include cryo-TEM, high- resolution TEM, tomography, diffraction, energy-dispersive x-ray spectroscopy, energy-filtered TEM, electron energy-loss spectroscopy, etc. The Atomic Force Microscopy (AFM) Lab, integrating both research and teaching functions, features 5 TT- AFMs made by AFMWorkshop. Users can study the surface topography of their samples at a nanometer or even sub-nanometer resolution. The Scanning Electron Microscopy (SEM) and e - beam writer Labs currently host a FEI Quanta450 environmental SEM with Nabity NPGS EBL system and an easy-to-use Hitachi S- 2600N microscope. • Location: the ground floor of the north wing of the Liquid Crystal and Materials Science Building. High - speed camera : • Phantom v210 from Vision Research • Frame rates >2000fps in full frame (1280 x 800) • 300,000 fps at a reduced frame size AFM assembly Workshop (02/2012@LCI) Microscopy Society of Northeastern Ohio Meeting (10/2013@LCI) The Specimen preparation Lab hosts a series of specimen preparation instruments for soft-matter materials. Available techniques include plunge freezing, high pressure freezing, cryo-ultramicrotomy, and freeze fracture. Bal-Tec BAF060 freeze fracture Leica EM Pact2 high pressure freezer Leica UC7/FC7 Cryo-ultramicrotome FEI Mark IV Vitrobot Plunge-freezer Initialized MSNO Microscopy Summer School (microscopy of soft-matter materials) in 2015: 60 attendees. Instructors: Min Gao (Kent State University, Cryo-techniques), Adam Smith (University of Akron, light microscopy), and Midori Hitomi (Cleveland Clinic, traditional biological EM) Represented MSNO to initialize interaction with high school students and teachers: two microscopy demos at CTSC College Fair (in Cleveland, top: live demo of a Table-Top AFM) and ACESS College Fair (in Akron, bottom: remote control of a FEI Quanta450 SEM) Basic TEM training Confocal Laser Scanning Microscope: • LEXT OLS 3100 from Olympus • 408 nm LD source • 3D profile reconstruction, Resolution: lateral - 200 nm; vertical - 10 nm. FTIR Microscope: • Vertex 70 from Brucker • FTIR, MidIR, NearIR, Transmission, Reflection, Microscope, Bench, Grazing Angle, ATR Goniometer : • Model 250 from Rame - Hart • Temperature can be varies up to 315°C (600°F) • The resolution of the angle measurement is 0.01° with an accuracy of +/- 0.1° Rheometer: HAAKE ™ MARS ™ II from Thermo Fisher Scientific Zeta potential and particle size analyzer: • ZetaPLUS from Brookhaven Instruments • Particle size (1 − 3,000 nm) • Zeta-potential (-220 to 220 mV) • Temperature varies from 10 to 70 °C Version: 5/2016 2 n m 2 n m TEM image of a Pt nanoparticle with resolved lattice spacing of 0.196 nm. A few application examples 2 nm Website: http://www.lcinet.kent.edu/organization/facility/characterization/index.php Contact: [email protected]; [email protected], 330-672-7999 Cryo - TEM of vesicles Freeze fracture TEM Nature Comm 4: 2635, 2013 Freeze fracture TEM image of twist - bend nematic CB7CB, revealing the ~8 nm pitch. E - beam lithography AFM image of a lithography pattern (30 x 30 microns). CEMOVIS (cryo -electron microscopy of vitreous section ) of lyotropic liquid crystals Environmental SEM: formation of nanoscale water droplets Tomography: 3D structure of graphene and nanorods 2 n m 2 n m TEM image of a Pt nanoparticle with resolved lattice spacing of 0.196 nm. 2 nm AFM: 3D structure inside DVD disk Environmental SEM: Ant AFM: EBL pattern E-SEM: pollen
Transcript of Characterization Facility - · PDF fileresolution TEM, tomography, diffraction, ......
Characterization Facilityat Liquid Crystal Institute of Kent State University
Introduction• The Liquid Crystal Institute (LCI) Characterization Facility, is an open access user facility serving the nearby academic and industrial communities and beyond.
Activities
Service• Serving 70+ academic groups (from Kent, Akron, Cleveland, Rootstown,
Columbus, Toledo, Cincinnati, OH and out-of-state) and industrial partners(Sherwin Williams, Lubrizol, ExxonMobil, Shire, KDI, Fischione, etc.).
Self-Use Training• Providing one-on-one and group training on most of the available instruments.• Popular training courses include basic AFM, basic SEM, basic TEM, cryo-TEM, TEM
specimen preparation of soft-matter materials, confocal laser scanningmicroscope, and high speed camera.
Other instruments include Zeta potential and particle size analyzer, FTIRspectrometer/microscope, rheometer, 2D polarimeter, chroma meter, Abberefractometer, differential scanning calorimeter (DSC), profilometer, polarized opticalmicroscope, etc.
Teaching and outreaching• Integrating lectures and lab-demos on TEM/SEM/AFM into 2 graduate and 1
undergraduate classes.
• Giving introductory/research seminars for academic institutions and localcompanies.
• Lab tour and demo available to public.• Promoting microscopy and characterization techniques.
• Working with academic societies/instrument manufacturers, and co-hostingmeetings, workshops and demos.
Fluid Dynamic Properties
• Providing a comprehensive series of instruments and analytical tools to characterize the following aspects of hard- and soft-matter materials and devices.
• The LCI Characterization Facility serves also as a center of hands-on training and education on advanced analytical techniques.
Atomic/nano-/micro-scale structure (TEM, SEM, SPM, Optical) Thermal and Phase Properties
Optical and Electro-optical Properties
Surface Properties
Mechanical and Rheological Properties …
Labs and Instruments
• Providing self-use, staff operation and collaboration for different users.
The Transmission Electron Microscopy (TEM) Lab features aversatile 200 kV microscope (FEI Tecnai G2 F20, 0.24 nm point-to-point resolution). Available techniques include cryo-TEM, high-resolution TEM, tomography, diffraction, energy-dispersive x-rayspectroscopy, energy-filtered TEM, electron energy-lossspectroscopy, etc.
The Atomic Force Microscopy (AFM) Lab, integratingboth research and teaching functions, features 5 TT-AFMs made by AFMWorkshop. Users can study thesurface topography of their samples at a nanometer oreven sub-nanometer resolution.
The Scanning ElectronMicroscopy (SEM) and e-beamwriter Labs currently host a FEIQuanta450 environmental SEMwith Nabity NPGS EBL systemand an easy-to-use Hitachi S-2600N microscope.
• Location: the ground floor of the north wing of the Liquid Crystal and Materials Science Building.
High-speed camera:• Phantom v210 from Vision Research• Frame rates >2000fps in full frame
(1280 x 800)• 300,000 fps at a reduced frame size
AFM assembly Workshop (02/2012@LCI)
Microscopy Society of Northeastern Ohio Meeting (10/2013@LCI)
The Specimen preparation Lab hosts a series of specimen preparation instruments forsoft-matter materials. Available techniques include plunge freezing, high pressurefreezing, cryo-ultramicrotomy, and freeze fracture.
Bal-Tec BAF060freeze fracture
Leica EM Pact2 high pressure freezer
Leica UC7/FC7Cryo-ultramicrotome
FEI Mark IV VitrobotPlunge-freezer
Initialized MSNO Microscopy Summer School (microscopy of soft-matter materials) in2015: 60 attendees. Instructors: Min Gao (Kent State University, Cryo-techniques), AdamSmith (University of Akron, light microscopy), and Midori Hitomi (Cleveland Clinic,traditional biological EM)
Represented MSNO to initialize interaction withhigh school students and teachers: two microscopydemos at CTSC College Fair (in Cleveland, top: livedemo of a Table-Top AFM) and ACESS College Fair(in Akron, bottom: remote control of a FEIQuanta450 SEM)
Basic TEM training
Confocal Laser Scanning Microscope:
• LEXT OLS 3100 from Olympus• 408 nm LD source• 3D profile reconstruction, Resolution:
lateral - 200 nm; vertical - 10 nm.
FTIR Microscope:• Vertex 70 from Brucker• FTIR, MidIR, NearIR, Transmission,
Reflection, Microscope, Bench,Grazing Angle, ATR
Goniometer:• Model 250 from Rame-Hart• Temperature can be varies up to
315°C (600°F)• The resolution of the angle
measurement is 0.01° with anaccuracy of +/- 0.1°
Rheometer:HAAKE™ MARS™ II from Thermo Fisher Scientific
Zeta potential and particle size analyzer:
• ZetaPLUS from Brookhaven Instruments• Particle size (1 − 3,000 nm)• Zeta-potential (-220 to 220 mV)• Temperature varies from 10 to 70 °C
Version: 5/2016
2 n m2 n m
TEM image of a Pt nanoparticle with
resolved lattice spacing of 0.196 nm. A few application examples
2 nm
Website: http://www.lcinet.kent.edu/organization/facility/characterization/index.php Contact: [email protected]; [email protected], 330-672-7999
Cryo-TEM of vesicles
Freeze fracture TEM
Nature Comm 4: 2635, 2013
Freeze fracture TEM image of twist-bend nematic CB7CB, revealing the ~8 nm pitch.
E-beam lithography
AFM image of a lithography pattern (30 x 30 microns).
CEMOVIS (cryo-electron microscopy of vitreous section)
of lyotropic liquid crystals
Environmental SEM: formation of nanoscale water droplets
Tomography: 3D structure of graphene and nanorods
2 n m2 n m
TEM image of a Pt nanoparticle with
resolved lattice spacing of 0.196 nm.
2 nm
AFM: 3D structure inside DVD disk
Environmental SEM: Ant
AFM: EBL pattern
E-SEM: pollen
