Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical...

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Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100 $ 100 $100 $100 $200 $200 $200 $200 $300 $300 $300 $300 $400 $400 $400 $400 $500 $500 $500 $500 Physical Requirements Chemical Requirements Growth of Bacterial Cultures Culture Media Measurement of Growth FINAL ROUND

Transcript of Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical...

Page 1: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Chapter 6 – Microbial Growth

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Physical Requirements

ChemicalRequirements

Growth of Bacterial Cultures

Culture Media

Measurement of Growth

FINAL ROUND

Page 2: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$100 Question

The optimum pH for most bacteria is

a. 1

b. 5

c. 7

d. 9

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ANSWER

Page 3: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$100 Answer

The optimum pH for most bacteria is

a. 1

b. 5

c. 7 (correct answer)

d. 9

BACK TO GAME

Page 4: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$200 Question

A psychrophile grows best at

a. 37°C

b. 0°C

c. 25°C

d. 15°C

BACK TO GAME

ANSWER

Page 5: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$200 Answer

A psychrophile grows best at

a. 37°C

b. 0°C (correct answer)

c. 25°C

d. 15°C

BACK TO GAME

Page 6: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$300 Question

Organisms that require high salt concentrations for growth are called

a. Halophiles

b. Extreme halophiles

c. Facultative halophiles

d. Microhalophiles

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ANSWER

Page 7: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$300 Answer

Organisms that require high salt concentrations for growth are called

a. Halophiles

b. Extreme halophiles (correct answer)

c. Facultative halophiles

d. Microhalophiles

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Page 8: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$400 Question

The energy source utilized by producers in the hydrothermal vents on the ocean floor is

a. Light

b. Sulfur

c. Organic molecules

d. Carbon dioxide

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ANSWER

Page 9: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$400 Answer

The energy source utilized by producers in the hydrothermal vents on the ocean floor is

a. Light

b. Sulfur (correct answer)

c. Organic molecules

d. Carbon dioxide

BACK TO GAME

Page 10: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$500 Question

In food preservation, salts and sugars are used because they create a

a. Lower pH

b. Hypertonic environment

c. Hypotonic environment

d. Lower osmotic pressure

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ANSWER

Page 11: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 1: Physical Requirements

$500 Answer

In food preservation, salts and sugars are used because they create a

a. Lower pH

b. Hypertonic environment (correct answer)

c. Hypotonic environment

d. Lower osmotic pressure

BACK TO GAME

Page 12: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$100 Question

Chemoautotrophs and photoautotrophs derive their carbon from

a. Carbon dioxide

b. Proteins

c. Lipids

d. Carbohydrates

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ANSWER

Page 13: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$100 Answer

Chemoautotrophs and photoautotrophs derive their carbon from

a. Carbon dioxide (correct answer)

b. Proteins

c. Lipids

d. Carbohydrates

BACK TO GAME

Page 14: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$200 Question

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. Chemosynthesis

b. Photosynthesis

c. Nitrogen fixation

d. Nitrogen synthesis

BACK TO GAME

ANSWER

Page 15: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$200 Answer

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. Chemosynthesis

b. Photosynthesis

c. Nitrogen fixation (correct answer)

d. Nitrogen synthesis

BACK TO GAME

Page 16: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$300 Question

An organism that uses oxygen but can

grow without oxygen is called a(n)

a. Obligate aerobe

b. Facultative aerobe

c. Aerobe

d. Aerotolerant anaerobe

BACK TO GAME

ANSWER

Page 17: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$300 Answer

An organism that uses oxygen but can

grow without oxygen is called a(n)

a. Obligate aerobe

b. Facultative aerobe (correct answer)

c. Aerobe

d. Aerotolerant anaerobe

BACK TO GAME

Page 18: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$400 Question

This organism is killed by atmospheric

oxygen:

a. Aerobe

b. Obligate aerobe

c. Obligate anaerobe

d. Aerotolerant anaerobe

BACK TO GAME

ANSWER

Page 19: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$400 Answer

This organism is killed by atmospheric oxygen:

a. Aerobe

b. Obligate aerobe

c. Obligate anaerobe (correct answer)

d. Aerotolerant anaerobe

BACK TO GAME

Page 20: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$500 Question

Biofilms reside in a matrix made up primarily of

a. Lipids

b. Hydrogel

c. Proteins

d. Polysaccharides

BACK TO GAME

ANSWER

Page 21: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 2: Chemical Requirements

$500 Answer

Biofilms reside in a matrix made up primarily of

a. Lipids

b. Hydrogel

c. Proteins

d. Polysaccharides (correct answer)

BACK TO GAME

Page 22: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$100 Question

Generation time can best be defined as

a. The length of time it takes for lag phase

b. The duration of log phase

c. The length of time it takes for a cell to divide

d. The minimum rate of doubling

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ANSWER

Page 23: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$100 Answer

Generation time can best be defined as

a. The length of time it takes for lag phase

b. The duration of log phase

c. The length of time it takes for a cell to divide (correct answer)

d. The minimum rate of doubling

BACK TO GAME

Page 24: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$200 Question

To cultivate anaerobic bacteria, one must use special media called

a. Reducing media

b. Oxidizing media

c. Differential media

d. Selective media

BACK TO GAME

ANSWER

Page 25: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$200 Answer

To cultivate anaerobic bacteria, one must use special media called

a. Reducing media (correct answer)

b. Oxidizing media

c. Differential media

d. Selective media

BACK TO GAME

Page 26: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$300 Question

Capnophiles must be grown in high concentrations of

a. Oxygen

b. Salt

c. Glucose

d. Carbon dioxide

BACK TO GAME

ANSWER

Page 27: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$300 Answer

Capnophiles must be grown in high concentrations of

a. Oxygen

b. Salt

c. Glucose

d. Carbon dioxide (correct answer)

BACK TO GAME

Page 28: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$400 Question

The culture medium that is designed to increase numbers of desired microbes to detectable levels is called

a. Complex

b. Selective

c. Enrichment

d. Differential

BACK TO GAME

ANSWER

Page 29: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$400 Answer

The culture medium that is designed to increase numbers of desired microbes to detectable levels is called

a. Complex

b. Selective

c. Enrichment (correct answer)

d. Differential

BACK TO GAME

Page 30: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$500 Question

The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called

a. Complex

b. Selective

c. Reducing

d. Differential

BACK TO GAME

ANSWER

Page 31: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 3: Growth of Bacterial Cultures

$500 Answer

The culture medium that is designed to suppress unwanted microbes while encouraging desired microbes is called

a. Complex

b. Selective (correct answer)

c. Reducing

d. Differential

BACK TO GAME

Page 32: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$100 Question

To neutralize acids as they are produced in a culture, what should be added to the culture medium?

a. Buffers

b. Salts

c. Sugars

d. PABA

BACK TO GAME

ANSWER

Page 33: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$100 Answer

To neutralize acids as they are produced in a culture, what should be added to the culture medium?

a. Buffers (correct answer)

b. Salts

c. Sugars

d. PABA

BACK TO GAME

Page 34: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$200 Question

The microbes that grow and multiply in or on a culture medium are referred to as a(n)

a. Culture

b. Inoculum

c. Specimen

d. Medium

BACK TO GAME

ANSWER

Page 35: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$200 Answer

The microbes that grow and multiply in or on a culture medium are referred to as a(n)

a. Culture (correct answer)

b. Inoculum

c. Specimen

d. Medium

BACK TO GAME

Page 36: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$300 Question

What can be added to a medium when it is desirable to grow bacteria on a solid medium?

a. Glucose

b. Magnesium sulfate

c. Potassium phosphate

d. Agar

BACK TO GAME

ANSWER

Page 37: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$300 Answer

What can be added to a medium when it is desirable to grow bacteria on a solid medium?

a. Glucose

b. Magnesium sulfate

c. Potassium phosphate

d. Agar (correct answer)

BACK TO GAME

Page 38: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$400 Question

A culture medium consisting of agar, human blood, and beef heart is a

a. Complex medium

b. Selective medium

c. Differential medium

d. Chemically defined medium

BACK TO GAME

ANSWER

Page 39: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$400 Answer

A culture medium consisting of agar, human blood, and beef heart is a

a. Complex medium (correct answer)

b. Selective medium

c. Differential medium

d. Chemically defined medium

BACK TO GAME

Page 40: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$500 Question

The source of nutrients in nutrient agar is

a. Agar

b. Peptone and sodium chloride

c. Peptone and beef extract

d. Nutrient

BACK TO GAME

ANSWER

Page 41: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 4: Culture Media

$500 Answer

The source of nutrients in nutrient agar is

a. Agar

b. Peptone and sodium chloride

c. Peptone and beef extract (correct answer)

d. Nutrient

BACK TO GAME

Page 42: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$100 Question

A spectrophotometer can be used to measure

a. Metabolic activity

b. Turbidity

c. Dry weight

d. Pathogenicity

BACK TO GAME

ANSWER

Page 43: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$100 Answer

A spectrophotometer can be used to measure

a. Metabolic activity

b. Turbidity (correct answer)

c. Dry weight

d. Pathogenicity

BACK TO GAME

Page 44: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$200 Question

Because of time considerations, which method is usually used to count the number of bacteria in milk?

a. The direct microscope count

method

b. The pour plate method

c. The spread plate method

d. The most probable number method

BACK TO GAME

ANSWER

Page 45: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$200 Answer

Because of time considerations, which method is usually used to count the number of bacteria in milk?

a. The direct microscope count

method (correct answer)

b. The pour plate method

c. The spread plate method

d. The most probable number method

BACK TO GAME

Page 46: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$300 Question

Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?

a. The most probable number method

b. Serial dilution

c. Filtration

d. Spread plate method

BACK TO GAME

ANSWER

Page 47: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$300 Answer

Which method is utilized to ensure that plates containing 25 to 250 colonies are counted?

a. The most probable number method

b. Serial dilution (correct answer)

c. Filtration

d. Spread plate method

BACK TO GAME

Page 48: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$400 Question

Microorganisms are particularly sensitive to adverse conditions during the

a. Lag phase

b. Death phase

c. Stationary phase

d. Log phase

BACK TO GAME

ANSWER

Page 49: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$400 Answer

Microorganisms are particularly sensitive to adverse conditions during the

a. Lag phase

b. Death phase

c. Stationary phase

d. Log phase (correct answer)

BACK TO GAME

Page 50: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$500 Question

Which of the following metabolic activities can be used to measure microbial growth?

a. Carbon dioxide consumption

b. Oxygen consumption

c. Glucose consumption

d. Salt consumption

BACK TO GAME

ANSWER

Page 51: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

Topic 5: Measurement of Growth

$500 Answer

Which of the following metabolic activities can be used to measure microbial growth?

a. Carbon dioxide consumption

b. Oxygen consumption

c. Glucose consumption (correct answer)

d. Salt consumption

BACK TO GAME

Page 52: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

FINAL ROUND Question

An experiment began with 5 cells and

ended with 160 cells. How many

generations did the cells go through?

a. 20

b. 6

c. 4

d. 5BACK TO GAME

ANSWER

Page 53: Chapter 6 – Microbial Growth $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Physical Requirements Chemical Requirements Growth of Bacterial.

FINAL ROUND Answer

An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through?

a. 20

b. 6

c. 4

d. 5 (correct answer)

BACK TO GAME