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165

ANNEXURE I

BUFFERS AND REAGNETS BCA assay SDS -10% stock

Chaps -0.5% stock Bicinchonic acid -9.8 ml Cupric sulphate -0.2 ml

Cell Fixative Gluteraldehyde - 2.5% in PBS (for SEM analysis) Paraformaldehyde - 2% Phosphate Buffered Saline

NaCl - 136mM KCL - 2.6mM Na2HPO4 - 10mM KH2PO4 - 1.76mM

Trypan Blue Trypan blue - 0.4% in PBS

Griess reagent (Anticoagulant citrate dextrose) (ACD)

Citric acid -20mM Sodium citrate -110 mM D glucose -5mM

166

AWARDS AND HONORS

University 5th Rank (M.Sc Polymer Chemistry, University of Kerala, 2004)

Council of Scientific and Industrial Research (CSIR) awarded Senior Research Fellowship

PUBLICATIONS 1. K.T. Shalumon, N.S. Binulal, M. Deepthy, R. Jayakumar, K. Manzoor, S. V. Nair. Preparation,

characterization and cell attachment studies of electrospun multi-scale poly (caprolactone) fibrous

scaffolds for tissue engineering. J Macromol. Sci. A: Pure and Appl. Chem. 48, 1, 2011, 21-30.

2. K.T. Shalumon, K.P. Chennazhi, H. Tamura, K. Kawahara, S.V. Nair, R. Jayakumar.

Fabrication of 3D nano, micro and micro/nano scaffolds of porous poly (Lactic acid) by

electrospinning and comparison of cell infiltration by Z-stacking/3D projection technique. IET

Nanobiotech., doi: 10.1049/iet-nbt.2011.0028.

3. K.T. Shalumon, P.R Sreerekha, D. Sathish, H.Tamura, S.V. Nair, K.P. Chennazhi, R.

Jayakumar. Hierarchically designed electrospun tubular scaffolds for cardiovascular applications.

J Biomed. Nanotech., 7, 5, 2011, 1-12.

4. K.T. Shalumon, N.S. Binulal, N. Selvamurugan, S.V. Nair, D. Menon, H. Tamura, T. Furuike,

R. Jayakumar. Electrospinning of carboxymethyl chitin/poly(vinyl alcohol) nanofibrous scaffolds

for tissue engineering applications. Carbohydr. Polym., 77, 4, 2009, 863-869.

5. K.T. Shalumon, K.H. Anulekha, C.M. Girish, R. Prasanth, S.V. Nair, R. Jayakumar. Single step

electrospinning of chitosan/poly(caprolactone) nanofibers using formic acid/acetone solvent

mixture. Carbohydr. Polym., 80, 2, 2010, 413-419.

6. K.T. Shalumon, K.H. Anulekha, K.P. Chennazhi, H. Tamura, S.V. Nair, R. Jayakumar.

Fabrication of chitosan/poly(caprolactone) nanofibrous scaffold for bone and skin tissue

engineering. Int. J. Biol. Macromol., 48, 4, 2011, 571-576.

7. K.T. Shalumon, K.H. Anulekha, Sreeja V. Nair, S.V. Nair, K.P. Chennazhi, R. Jayakumar.

Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound

dressings . Int. J. Biolo. Macromol., 49, 3, 2011, 247-254.

8. K.T. Shalumon, M. Jayabalan. Studies on biodegradation of crosslinked hydroxy terminated-

poly (propylene fumarate) and formation of scaffold for orthopedic applications. J. Material

Sci.: Materials in Med., 20, 1, 2009, S161-71.

167

9. M. Jayabalan, K. T. Shalumon, M.K. Mitha. Injectable biomaterials for minimally invasive

orthopedic treatments. J. Material Sci.: Materials in Med., 20, 6, 2009, 1379-1386.

10. M. Jayabalan, K.T. Shalumon, M.K Mitha, K. Ganesan, M. Epple. Effect of hydroxyapatite on

the biodegradation and biomechanical stability of polyester nanocomposites for orthopaedic

applications. Acta Biomater., 6,3, 2010, 763-775.

11. M. Jayabalan, K. T. Shalumon, M. K. Mitha, K. Ganesan, M. Epple. The effect of radiation

processing and filler morphology on the biomechanical stability of a thermoset polyester

composite. Biomed. Materials, 5, 2, 2010, 025009, M. Jayabalan.

12. N.S. Binulal, M. Deepthy, N. Selvamurugan, K.T. Shalumon, S. Suja, Ullas Mony, R.

Jayakumar, S.V. Nair. Role of Nanofibrous Poly(Caprolactone) Scaffolds in Human

Mesenchymal Stem Cell Attachment and Spreading for In Vitro Bone Tissue Engineering-

response to Osteogenic Regulators. Tissue Eng, A. 16, 2, 2010, 393-404.

13. L.R. Lakshman, K. T. Shalumon, Sreeja V. Nair, R. Jayakumar, S.V. Nair. Preparation of Silver

Nanoparticles Incorporated Electrospun Polyurethane Nanofibrous Mat for Wound Dressing. J.

Macromol. Sci. A Pure Appl Chem, 47,10,2010, 1012-1018.

14. B.T. Midhun, K.T. Shalumon, K Manzoor, R Jayakumar, S.V Nair, M Deepthy. Preparation of

budesanoid loaded Polycaprolactone Nanobeads by Electrospraying for Controlled Drug Release.

J. Biomater Sci. Polym Ed, 2010.

15. H. Nagahama, V.V. Divya Rani, K.T. Shalumon, R. Jayakumar, S.V. Nair, S. Koiwa, T.

Furuike, H. Tamura. Preparation, characterization, bioactive and cell attachment studies of α-

chitin/gelatin composite membranes. Int. J. Biol. Macromol., 44, 4, 2009, 333-337.

16. R. Jayakumar, V.V. Divya Rani, K.T.Shalumon, P.T. Sudheesh Kumar, S.V. Nair, T. Furuike,

H. Tamura. Bioactive and osteoblast cell attachment studies of novel α- and β-chitin membranes

for tissue-engineering applications. Int. J. Biol. Macromol., 45, 3, 2009, 260-264.

17. K. Madhumathi, K.T. Shalumon, V.V. Divya Rani, H. Tamura, T. Furuike, N. Selvamurugan,

S.V. Nair, R. Jayakumar. Wet chemical synthesis of chitosan hydrogel–hydroxyapatite composite

membranes for tissue engineering applications. Int. J. Biol. Macromol., 45, 1, 2009, 12-15.

18. K. Madhumathi, N.S. Binulal, H. Nagahama, H. Tamura, K.T. Shalumon, N. Selvamurugan,

S.V. Nair, R. Jayakumar. Preparation and characterization of novel β-chitin–hydroxyapatite

composite membranes for tissue engineering applications. Int. J. Biol. Macromol., 44, 1, 2009, 1-

5.

168

CONFERENCE PRESENTATIONS

1. K.T. Shalumon, N.S. Binulal, U.S. Sajeev, Deepthy Menon, K. Manzoor, R.Prasanth and

Shantikumar Nair. “Novel Single-step Electrospun 3-D Nanocomposite Multiscale and

Multifunctional Scaffolds for Tissue Engineering Applications”. Nanoscience and

Nanotechnology initiative (NSNT) Biannual review meeting, March 12-15, 2009, SN Bose

institute, Kolkota, India.

2. Shalumon K T, Binulal N S, Jayakumar R, Deepthy menon, Selvamurugan N and Shanti V

Nair. “Preparation of Carboxymethyl Chitin/poly(vinyl alcohol)nanofibers for tissue engineering

applications”. NANOBIO- 2009, February 17-19, 2009, Amrita Centre for Nanosciences, Amrita

Institute for Medical Sciences and Research Centre, Cochin, Kerala, India.

3. Shalumon K.T and Muthu Jayabalan. “Degradation profile of polypropylene fumarate based

bone cements”. Polymers for Advanced Technologies (MACRO- 2006), December 17-20, 2006,

National Chemical Laboratories, Pune, India.