CHAPTER 3 Amino Acids, Peptides, Proteins Structure and naming of amino acids Structure and...
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Transcript of CHAPTER 3 Amino Acids, Peptides, Proteins Structure and naming of amino acids Structure and...
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CHAPTER 3 Amino Acids, Peptides,
Proteins
• Structure and naming of amino acids• Structure and properties of peptides• Ionization behavior of amino acids and peptides• Purification and assay methods • Peptide sequencing and chemical synthesis• Protein sequence analysis
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Proteins: Main Agents of Biological Function
• Catalysis:–enolase (in the glycolytic pathway)
–DNA polymerase (in DNA replication)
• Transport:–hemoglobin (transports O2 in the blood)
–lactose permease (transports lactose across the cell membrane)
• Structure:–collagen (connective tissue)
–keratin (hair, nails, feathers, horns)
• Motion:–myosin (muscle tissue)
–actin (muscle tissue, cell motility)
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Amino Acids: Building Blocks of Protein
• Proteins are heteropolymers of -amino acids• Amino acids have properties that are well
suited to carry out a variety of biological functions:– Capacity to polymerize– Useful acid-base properties– Varied physical properties– Varied chemical functionality
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Amino Acids: Atom Naming
• Organic nomenclature: start from one end• Biochemical designation: start from
-carbon and go down the R-group
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Most -Amino Acids are Chiral• The -carbon has always
four substituents and is tetrahedral
• All (except proline) have an acidic carboxyl group, a basic amino group, and an alpha hydrogen connected to the -carbon
• Each amino acid has an unique fourth substituent R
• In glycine, the fourth substituent is also hydrogen
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Amino Acids: Classification
Common amino acids can be placed in five basic groups depending on their R substituents:
• Nonpolar, aliphatic (7)
• Aromatic (3)
• Polar, uncharged (5)
• Positively charged (3)
• Negatively charged (2)
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Aliphatic Amino Acids
• http://en.wikipedia.org/wiki/File:Aa.svg
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Aromatic Amino Acids
• http://en.wikipedia.org/wiki/File:Aa.svg
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Charged Amino Acids
• http://en.wikipedia.org/wiki/File:Aa.svg
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Polar Amino Acids
• http://en.wikipedia.org/wiki/File:Aa.svg
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Special Amino Acids
• http://en.wikipedia.org/wiki/File:Aa.svg
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Not incorporated by ribosomes
Arise by post-translational modifications of proteins
Reversible modifications, esp. phosphorylation is important in regulation and signaling
Uncommon Amino Acids in Proteins
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The Genetic Code is organized by Amino Acid Properties
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Ionization
At acidic pH, the carboxyl group is protonated and the amino acid is in the cationic form
At neutral pH, the carboxyl group is deprotonated but the amino group is protonated. The net charge is zero; such ions are called Zwitterions
At alkaline pH, the amino group is neutral –NH2 and the amino acid is in the anionic form.
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Substituent effects on pKa Values-carboxy group is much more acidic than in carboxylic acids-amino group is slightly less basic than in amines
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Amino Acids Can Act as Buffers
Amino acids with uncharged side-chains, such as glycine, have two pKa values:
The pKa of the -carboxyl group is 2.34
The pKa of the -amino group is 9.6
It can act as a buffer in two pH regimes.
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Amino Acids Carry a Net Charge of Zero at a Specific pH
•Zwitterions predominate at pH values between the pKa values of amino and carboxyl group
•For amino acid without ionizable side chains, the Isoelectric Point (equivalence point, pI) is
• At this point, the net charge is zero
– AA is least soluble in water
– AA does not migrate in electric field
221 pKpK
pI
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Ionizable Side Chains Can Show Up in Titration Curves
• Ionizable side chains can be also titrated
• Titration curves are now more complex
• pKa values are discernable if two pKa values are more than two pH units apart
Why is the side-chain pKa so much higher?
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How to Calculate the pI When the Side-chain is Ionizable?
• Identify species that carries
a net zero charge
• Identify pKa value that
defines the acid strength of
this zwitterion: (pK2)
• Identify pKa value that
defines the base strength of
this zwitterion: (pKR)
• Take the average of these
two pKa values
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Peptides and Peptide bonds Peptide bond in a di-peptide
“Peptides” are small condensation products of amino acids
They are “small” compared to proteins (di, tri, tetra… oligo-)
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Peptide Ends are Not the Same
Numbering starts from the amino terminus
AA1 AA2 AA3 AA4 AA5
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The Three Letter Code
• Naming starts from the N-terminus
• Sequence is written as:
Ala-Glu-Gly-Lys
• Sometimes the one-letter code is used:
AEGK
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Peptides: A Variety of Functions
• Hormones and pheromones:– insulin (think sugar)– oxytocin (think childbirth)– sex-peptide (think fruit fly mating)
• Neuropeptides– substance P (pain mediator)
• Antibiotics:– polymyxin B (for Gram - bacteria)– bacitracin (for Gram + bacteria)
• Protection, e.g. toxins– amanitin (mushrooms)– conotoxin (cone snails)– chlorotoxin (scorpions)
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Proteins are:
• Cofactor is a general term for functional non-amino acid component – Metal ions or organic molecules
• Coenzyme is used to designate an organic cofactors – NAD+ in lactate dehydrogenase
• Prosthetic groups are covalently attached cofactors – Heme in myoglobin
• Polypeptides (covalently linked -amino acids) + possibly – • cofactors, • coenzymes, • prosthetic groups, • other modifications
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Polypeptide Size in Some Proteins
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Classes of Conjugated Proteins
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Peptides and Proteins- Burning Questions
Sequence and composition?
Three-dimensional structure?
Folding Mechanism?
Biochemical role?
Functional regulation?
Molecular interactions with small and macro-molecules?
Structural and sequence relatives?
Cellular and sub-cellular localization?
Physical and chemical properties?
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Purification – Fractionation of Protein Mixtures
• Separation relies on differences in physico-chemical properties– Solubility – Selective Precipitation (Centrifugation)– Thermal stability -- – Charge --Electrophoresis, Isoelectric Focusing, IEC– Size – Dialysis, Sedimentation (Centrifugation), GFC– Affinity for a ligand – “Pull down” assays (Centrifugation),
AC– Hydrophobicity (HIC)
• Chromatography is commonly used for preparative separation
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http://www.salinesystems.org/content/figures/1746-1448-4-1-2-l.jpg
Protein Fractionation
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Separation by Charge
•Ion Exchange Chromatography•Anion exchange
Matrix positive
Proteins negative
Displaced by anions
•Cation exchange – Opposite
• pH determines net charge on Proteins
•Salt concentration gradient
•Native gel electrophoresis
•Iso-electric Focusing
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Separation by Size
• Size exclusion (Gel Filtration) Chromatography– Loading vol. <5% of
column volume
– Samples diluted
• Dialysis or Centrifugal concentrators
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Separation by Affinity
• Affinity Chromatography
• Free Ligand-Beads -- centrifugation
• Ligand-Magnetic-Beads
• Immuno-assays on solid supports
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Electrophoresis for Protein Analysis
Separation in analytical scale is commonly done by electrophoresis
– Electric field pulls proteins according to their charge
– Gel matrix hinders mobility of proteins according to their size and shape
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SDS PAGE: Molecular Weight• SDS – sodium dodecyl
sulfate – a detergent
• SDS micelles binds to, and unfold all the proteins– SDS gives all proteins an
uniformly negative charge
– The native shape of proteins does not matter
– Rate of movement will only depend on size: small proteins will move faster
-
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Protein Sequencing
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Spectroscopic Detection of Aromatic Amino Acids
• The aromatic amino acids absorb light in the UV region
• Proteins typically have UV absorbance maxima around 275-280 nm
• Tryptophan and tyrosine are the strongest chromophores
• Concentration can be determined by UV-visible spectrophotometry using
Beers law: A = ·c·l
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Chapter 3: Summary
In this chapter, we learned about:
• The many biological functions of peptides and proteins
• The structures and names of amino acids found in proteins
• The ionization properties of amino acids and peptides
• The methods for separation and analysis of proteins
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Nonpolar, Aliphatic R Groups
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Aromatic R Groups
Also Hydrophobic
These amino acid side chains absorb UV light at 270-280 nm
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Polar, Uncharged R
GroupsThese amino acids side chains can form hydrogen bonding
Cysteine can form disulfide bonds
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Basic R Groups
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Acidic R Groups