Chapter 22 Biosynthesis of amino acids, nucleotides and related molecules 1. Reduction (fixation) of...
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Transcript of Chapter 22 Biosynthesis of amino acids, nucleotides and related molecules 1. Reduction (fixation) of...
Chapter 22
Biosynthesis of amino acids, nucleotides and related
molecules
1. Reduction (fixation) of N2 into ammonia (NH3 or NH4+)
2. Synthesis of the 20 amino acids.
3. Synthesis of other biomolecules from amino acids
4. The de novo pathways for purine and pyrimidine biosynthesis.
5. The salvage pathways for purine and pyrimidine reuse.
1. The nitrogenase complex in certain bacteria (diazotrophs,固氮生物 ) cat
alyzes the conversion of N2 to NH3 • The nitrogen in amino acids, purines, pyrimidines and other
biomolecules ultimately comes from atmospheric nitrogen.• Cyanobacteria ( 蓝藻细菌 , photosynthetic) and rhizobia
( 根瘤菌 , symbiont) can fix N2 into NH3.
• The reduction of N2 to NH3 is thermodynamically favorable :
• N2 + 6e- + 6H+ 2NH3 G`o = -33.5kJ/mol• But kinetically unfavorable: the bond energy for the triple b
ond in N2 is 942 kJ/mol.
• The nitrogenase ( 固氮酶 ) complex mainly consists of two types of enzymes: the dinitrogenase and the dinitrogenase reductase.
• The dinitrogenase (containing molybdenum ,钼 , thus called the MoFe protein) is a tetramer of two different subunits, containing multiple 4Fe-4S centers and two Mo-Fe clusters.
• The dinitrogenase reductase (also called the Fe protein) is a dimer of two identifcal subunits, containing a single 4Fe-4S redox center.
• The nitrogenase complex is highly conserved among different diazotrophs.
Cyanobacteria and Rhizobia can fix N2 into ammonia
Rhizobia exist innodules ofleguminous plants
The nitrogenase complex
The dinitrogenase (tetramer)
The dinitrogenasereductase (dimer)
The dinitrogenasereductase (dimer)
ADP
ADP
4Fe-4S
4Fe-4S
4Fe-4S(P-cluster)
Fe-Mo cofactor
3Fe-3S
3Fe-3SMo
2. Electrons are transferred through a series of carriers to N2 for its reduct
ion on the nitrogenase complex• Eight electrons are believed to be needed for eac
h round of fixation reaction: with six for reducing one N2 and two for reducing 2 H+ (to form H2).
• The electrons mainly come from reduced ferredoxin (from photophosphorylation) or reduced flavodoxin (from oxidative phosphorylation) and are transferred to dinitrogenase via dinitrogenase reductase.
• For each electron to be transferred from dinitrogenase reductase to dinitrogenase, two ATPs are hydrolyzed causing a conformational change which reduces the electron affinity for the reductase (i.e., an increased reducing power).
• The oxidized and reduced dinitrogenase reductase dissociates from and associates with the dinitrogenase, respectively.
• The nitrogenase is in imperfect enzyme: H2 is formed along with NH3.
• The overall reaction catalyzed is:
• N2 + 8H+ +8e- + 16ATP + 16H2O
• 2NH3 + H2 + 16ADP + 16Pi
Electrons are transferredto N2 bound in theactive site of dinitrogenasevia ferredoxin/flavodoxinand dinitrogenase reductase
N2 is believed to bind tothe cavity of the Fe-Mocofactor of the dinitrogenase active site.3Fe-3S
3Fe-3S
3. The nitrogenase complex is extremely labile to O2 and various protective
mechanisms have evolved
• Some diazotrophs exist only anaerobically.
• Some cyanobacterial cells develop thick walls to prevent O2 from entering.
• The bacteria in root nodules are isolated from O2 by being bathed in a solution of the oxygen-binding protein leghemoglobin.
Leghemoglobin, produced in legume plants, has a high affinity to O2 and protects thenitrogenase complex in rhizobia
4. Reduced nitrogen in the form of NH4
+ is assimilated into amino acids via a two-enzyme pathway
• First NH4+ is added to the side chain of glutamate to
form glutamine in an ATP-dependent reaction catalyzed by glutamine synthetase.
• Then the side chain amino group of Gln is further transferred to -ketoglutarate to form Glu in a reaction catalyzed by glutamate synthase, an enzyme only present in bacteria and plants, not in animals.
• The amide group of Gln is a source of nitrogen in the synthesis of a variety of compounds, such as carbamoyl phosphate, Trp, His, glucosamine-6-P, CTP, and AMP.
• The amino groups of most other amino acids are derived from glutamate via transamination.
Newly fixed nitrogenin the form of NH4
+ isfirst incorporated intoglutamate to form glutamine
e
The side chainamino group ofglutamine is thentransferred to-ketoglutarate to form Glu
5. The bacterial Glutamine synthetase is a central control point in nitroge
n metabolism
• The bacterial glutamine synthetase has 12 identical subunits (each having an independent active site) arranged as two hexagonal rings.
• Each subunit of the enzyme is accumulatively inhibited by at least eight allosteric effectors.
• In addition the enzyme is more susceptible to the allosteric inhibition by having Tyr397 residue modified by adenylylation.
• The addition and removal of the AMP group to the glutamine synthetase are catalyzed by two active sites of the same bifunctional adenylyltransferase (AT).
• The substrate specificity of AT was found to be controlled by a regulatory protein, PII.
• The activity of PII, in turn, is regulated by the uridylylation of a specific Tyr residue: PII-UMP stimulates the adenylylation activity of AT, however, the unmodified PII stimulates the deadenylylation activity of AT.
• The addition and removal of UMP to PII, in turn, are again catalyzed by two active sites of the same protein, uridylyltransferase (UT): -ketoglutarate and ATP stimulate the uridylylation, however, Gln and Pi stimulate the deuridylylation (thus adenylylation of AT, inactivating glutamine synthetase).
The bacterial glutamine synthetasehas 12 subunits arranged as tworings of hexamers
Activesites
Tyr397
(adenylylation site)
The glutamine synthetase is accumulativelyinhibited by at least 8allosteric effectors,mostly end productsof glutaminemetabolism
A specific Tyr residue in bacterial glutamine synthetase can be reversiblyadenylylated
6. Amidotransferases catalyze the transfer of the amide amino group from
Gln to other compounds• All of the enzymes contain two structural domains:
one binding Gln, the other binding the amino group accepting substrate.
• A Cys residue is believed to act as a nucleophile to cleave the amide bond, forming a covalent glutamyl-enzyme intermediate with the NH3 produced remain in the active site and react with the second substrate to form an aminated product.
A proposedactionmechanism foramidotransferases
7. The 20 amino acids are synthesized from intermediates of glycolysis, the citric acid cycle, or pentose phosphate pathway
• The nitrogen is provided by Glu and Gln.• As in amino acid degradation, some of the synthetic
pathways (e.g., for Ala, Glu, and Asp) are very simple, but others (e.g., the aromatic ones) are very complex.
• Most bacteria and plants can synthesize all 20, but mammals can only synthesize about 10, with 10 being “essential”, needed in the diet.
• The pathways for the biosynthesis of the 20 amino acids can be categorized into six families according to the metabolic precursors used.
Intermediates of glycolysis,the citric acid cycle, and pentose phosphate pathwayserve as precursors for synthesizing the 20 standardamino acids.
+ acetyl-CoA
+ pyruvate +Asp
+ ATP Gln
8. -ketoglutarate is the common precursor for the biosynthesis of Glu, Gl
n, Pro, and Arg in bacteria• To make Glu, the amino group can be from the side
chain of Gln (catalyzed by an amidotransferase, glutamate synthase in bacteria) or ammonia (catalyzed by glutamate dehydrogenase in vertebrates).
• Gln is made from Glu by obtaining an amino group from ammonia in a reaction catalyzed by glutamine synthetase.
• Proline is synthesized from Glu via one step of phosphorylation (activation), two steps of enzyme-catalyzed reductions (using NADPH) and one step of nonenzymatic cyclization reaction (forming a Schiff base).
• To make Arg, ornithine is first made from Glu with a few steps similar to that of Pro synthesis, except that an acetyl group is first added to the -amino group of glutamate to protect the spontaneous Schiff base formation between the aldehyde group and the amino group and removed after an amino group is transferred to the aldehyde group.
• Arg is then synthesized from ornithine using steps similar to those of urea cycle in bacteria.
• Arg from the diet can be converted to Pro in mammals by being converted to ornithine first using the urea cycle enzymes and then to 1-pyrroline-5-carboxylate by ornithine -aminotransferase.
• Similarly, Arg can be formed from glutamate -semialdehyde (an intermediate of Pro synthesis) also by using ornithine -aminotransferase.
Glu is synthesized from -ketoglutarate by an amination reaction with amino groups obtained from Gln or ammonia
Gln is synthesized from Gluby obtaining an amino groupfrom ammonia in a reactioncatalyzed by glutaminesynthetase
Glutaminesynthetase
Glutamamatekinase
Glutamatedehydrogenase
spontaneous
Pyrroline carboxylatereductase
aminotransferase
Acetylglutamatesynthase
9. Ser, Gly, and Cys are all derived from 3-phosphoglycerate
• Ser is synthesized (in all organisms) from 3-phosphoglycerate via NAD+-dependent oxidation, PLP-dependent transamination, and dephosphorylation reactions.
• Gly is either derived from Ser by removal of the side chain -carbon (catalyzed by serine hydroxymethyltransferase, using PLP and H4 folate) or synthesized (in vertebrate liver cells) from CO2 and NH4
+ (catalyzed by glycine synthase, using N5,N10-methylene H4 folate).
• In plants and bacteria, Cys is made from Ser by replacing the side chain –OH group (after being activated first by acetylation) by an –SH group using sulfide ( 硫化物 ) reduced from sulfate ( 硫酸盐 ).
• In mammals, Cys is made from Met (providing the sulfur atom after being converted to homocysteine) and Ser (providing the carbon skeleton).
Ser is synthesized from 3-phosphoglycerate via oxidation, transamination, and dephosphorylation reactions
Phosphoserine aminotransferase
Gly is either derived from Ser by removal of the side chain -carbon or synthesized (in vertebrate liver cells) from CO2 and NH4
+
Serine hydroxymethyltransferase
Glycine Synthase
Cys is derived from Ser via a two-step pathway using sulfide in plants and bacteria
Cys is synthesized from homocysteine(which is derivedfrom Met) and Serin mammals
(胱硫醚 - 合成酶 )
(胱硫醚 - 裂解酶 )
Homocysteine (高半胱氨酸)
Homocysteine is derived from Met via S-adenosylmethionine and S-adenosylhomocysteine intermediates
Methionine adenosyltransferase
Methyl transferase
Methionine synthase
Hydrolase
Homocysteine
Met ATP
10. Ala, Val, and Leu are derived from pyruvate
• Ala is synthesized from pyruvate via a simple transamination reaction (with amino group donated by Glu).
• Val is synthesized from the transferring of a two-carbon unit to a pyruvate, forming -acetolactate, which is then converted via isomerization, reduction, dehydration, and transamination.
• The immediate precursor of Val, -keto-isovalerate, is converted to Leu via four steps of reactions: the addition of an acetyl group, a position switching of an –OH group, an oxidative decarboxylation, and a transamination reaction.
Acetohydroxyacid synthase
Acetohydroxyl acidisomeroreductase
Dihydroxy aciddehydratase
Valineaminotransferase
Val
Val is synthesized from two pyruvates
Leu is synthesized from -keto-isovalerate
Synthase
Isomerase
Dehydrogenase
Aminotransferase
Leu
11. Asp, Asn, Thr, Met are all derived from oxaloacetate
• Asp is synthesized from oxaloacetate via a simple transamination reaction.
• Asn is synthesized from Asp by an amidation ( 酰胺化 ) reaction with Gln donating the NH4
+(catalyzed by an amidotransferase).
• Thr and Met are all derived from Asp, branching off after Asp is converted to aspartate -semialdehyde (in a way similar to Glu to glutamate -semialdehyde conversion).
• Thr is synthesized from aspartate -semialdehyde via homoserine.
• Met is also synthesized from homoserine: it is first activated via succinylation; the accepting the sulfur atom from cysteine and methyl group from N5-methyl H4 folate.
Asp is synthesized from oxalocaetate by a simple transamination
Transaminase
Asn is synthesized from Asp by transferringan amino group from the amide group of Gln
Asparaginesynthetase
Asp is first converted to aspartate--semialdehyde which is then used to synthesize Thr, Met, and Lys.
Homoserinedehydrogenase
Homoserine kinase
Threoninesynthase
Thr is synthesized fromAsp via homoserine
Homoserine acyltransferase
Cystathionine--synthase
Cystathionine--lyase
Methionine syntase
Met is also synthesized from homoserine via cystathionine and homocysteine
Met
12. Lys and Ile are derived from oxaloacetate and pyruvate
• Lys is synthesized from aspartate -semialdehyde (which is derived from Asp) and pyruvate (contributing to the two carbons at positions of and of Lys), with glutamate donating the -amino group.
• Ile is made from -ketobutyrate (the dehydration/deamination product of Thr) and pyruvate, using the same set of enzymes as that for Val synthesis.
Dihydropicolinatesynthase
Dihydropicolinatesynthase
Lys is synthesized from aspartate--semialdehyde and pyruvate
Dehydrogenase
Synthase
Aminotransferase
Obtaining the -amino group from Glu
Desuccinylase
Epimerase
Decarboxylase
Lys
Removing the carboxyl groupof the condensed pyruvate
Acetolactatesynthase
Acetolactatesyntase
Acetohydroxy acidisomeroreductase
Acetohydroxy acidisomeroreductase
Dihydroxyl aciddehydratase
Valineaminotransferase
Pyruvate
Val
isopropylmalatesynthase Isopropylmalate
isomerase
Dehydrogenase
Leucineaminotransferase
LeuIle
Thr Ile is made from Thr andPyruvate using the same set Of enzymes for Val synthesis
13. The aromatic amino acids are derived from phophoenoylpyruvate and
erythrose 4-phosphate• All aromatic amino acids share the first seven reacti
ons for biosynthesis, up to the formation of chorismate ( 分支酸 ).
• All the carbons of the aromatic ring are derived from PEP and erythrose 4-P: ring closure follows the condensation reaction; which in turn is followed by step-wise double bond introduction (by dehydration).
• A second PEP enters to make chorismate.• Chorismate can be converted to anthranilate (by accepting
an amino group from Gln and releasing a pyruvate) or prephenate (by switching the position of the PEP component via a Claisen rearrangement) by the catalysis of anthranilate synthase or chorismate mutase respectively.
• Anthranilate ( 邻氨基苯甲酸 ) can be further converted to Trp via another four steps of reaction: accepting two carbons from 5-phosphoribosyl-1-pyrophosphate (PRPP), and three carbons from Ser.
• Prephenate ( 预苯酸 ) can be converted to Phe by releasing the carboxyl and hydroxyl groups from the ring, and accepting an amino group from Glu via a transamination reaction.
• Prephenate can also be converted to Tyr by an oxidative decarboxylation and a transamination reaction.
four steps
three steps
one stepone step
four stepstwo steps two steps
The biosynthesis of all aromatic amino acidsshare the first seven steps of reactions
The 2nd PEPenter here
Ring formationstep
1st doublebond
2nd doublebond
Chorismate
Chorismate, formed from the condensed product ofPEP and erythrose 4-P, is the common precursor of Trp, Tyr and Phe.
Chorismate is converted to Trp byaccepting an amino group from Gln, two carbons from 5 phosphoribosyl-1-pyrophosphate (PRPP), and three carbons from Ser.
( 邻氨基苯甲酸 )
Synthase
Transferase
Isomerase
Synthase
TrpSynthase
Chorismate can also beconverted to prephenatewhich serve as the common precursor of Phe and Tyr biosynthesis
Claison rearrangementChorismate
mutase
Dehydrogenase dehydratase
14. Tryptophan synthase catalyzes the conversion of indole-3-glycerol phosphate to Trp with indole as an inter
mediate• The tetrameric enzyme has two and two subunit
s.• Indole-3-glycerol phosphate enters the subunits (t
he “lyase”) and is cleaved to form indole and glyceraldehyde-3-P.
• Serine enters the subunits, forms a Schiff base with PLP, and is dehydrated to form a PLP-aminoacrylate adduct.
• The indole intermediate formed on the a subunit then enters the active site of the subunit via a “substrate channel”, where it condenses with the PLP-aminoacylate intermediate to form a ketimine ( 酮亚胺 ) intermediate, which is then converted to a aldimine ( 醛亚胺 ) intermediate.
• The aldimine intermediate is then hydrolyzed to form Trp.
The and subunits of tryptophansynthase have different enzymaticactivities with substrate channeling
A ketimine An aldimine
The hydrophobic indole is channeled from the to the subunits in tryptophan synthase
15. Histidine is derived from three precursors: 5-phosphoribosyl 1-pyro
phosphate, ATP and Gln• The synthesis begins with the condensation of ATP
and PRPP: N-1of the purine ring is linked to the C-1 of the ribose.
• For the final synthesis of Histidine, PRPP contributes five carbons, ATP contributes one nitrogen and one carbon (both from the purine ring), Gln contributes one nitrogen, and Glu contributes the 3rd nitrogen (of the -amino group).
• The unusual synthesis of histidine from a purine is taken as evidence supporting the hypothesis that life originated from RNA: the synthetic pathway is considered as a “fossil” of the transition from RNA to the more efficient protein-based life forms.
Transferase
Hydrolase
The synthesis of histidine begins with the condensation of PRPP and ATP: The C-1 ofthe ribose ring is linked to the N-1 of the purinering.
Hydrolase
IsomeraseAldose
Ketose
Amidotransferase
Aminotransferase Dehydrogenase
Dehydratase
Histidine is made from PRPP, ATP, Gln and Glu.
Ring opens betweenN-1 and C-6
Cleaved betweenC-2 and N-3
A purine!
16. Several feedback inhibition mechanisms are found to work in regulati
ng amino acid biosynthesis• The first case of allosteric feedback inhibition was
discovered in studying Ile biosynthesis in E. coli • The enzyme catalyzing the first reaction (threonin
e dehydratase) is inhibited by the end product (Ile) in a synthetic pathway.
• Several intricate feedback inhibition mechansims have been found in branched pathways for amino acid biosyntheses.
• In enzyme multiplicity, several isozymes are present to catalyze a common step of reaction: each isozyme responds to a different allosteric modulator, avoiding the inhibition of a common reaction by only one end product.
• In concerted inhibition, one enzyme is inhibited by two or more than two modulators, with effect more than additive.
• In sequential feedback inhibition, the end products inhibit enzymes catalyzing the branching points only, while the initial committing step is inhibited by common precursors of the end products.
The first case of allosteric feedback inhibition was discovered in studying Ile synthesis in E. coli
In branching pathways leading tothe synthesis of multiple end products from common precursors,several forms of feedback inhibitionmechanisms are used to coordinatelyregulate the synthesis of all the amino acids.
Several intricate feedback inhibition mechansims have been found in branched pathways for amino acid biosyntheses
Sequential
Concerted
Enzyme multiplicity
High level of one (e.g., Y) should not prevent the synthesis of the other (e.g., Z).