Chapter 11
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Transcript of Chapter 11
I. Principles of membrane transport - protein-free lipid bilayers are highly impermeable to ions- two main classes of membrane transport proteins- transporters ( also called carriers or permeases) and channels- active transport – mediated by transporters coupled to an energy source- passive transport – mediated by all channels and many transporters
II. Transporters and passive transport- glucose transporters
III. Transporters and active transport - coupled transporters; ATP-driven pumps; light-driven pumps- uniporters; symporters; antiporters- Na+-glucose cotransporter – coupled transport; symporter- lactose permease - coupled transport; symporter- three classes of ATP-driven pumps
- Ca2+ pump- Na+-K+ pump- ABC transporters
IV. Ion channels - ion channels are selective and gated- membrane potential- K+- channel- aquaporins- neuron- voltage-gated Na+-channels- transmitter-gated ion channels- neuromuscular transmission
Ion concentration differences across the lipid bilayer are useful for:1. Driving various transport processes2. Conveying electrical signals in electrically excitable cells3. Making most of the cell’s ATP4. Cell signaling
The rate at which a molecule diffuses across a synthetic lipid bilayer depend on its size and solubilityThe smaller the molecule and the less polar it is, the more rapidly it diffuses across the bilayer
Permeability coefficients (in cm/sec)through synthetic lipid bilayers
Product of the concentration difference(in mol/cm3) and permeability coefficient(in cm/sec) gives the flow of solute inmoles per second per square centimeter of membrane
Two main classes of membrane transport proteins: Transporters and Channels
All these proteins are multi-pass transmembrane proteins1. Transporters bind to a specific solute and undergo a series of conformational changes.
2. Channel proteins interact with the solute much more weakly; form aqueous pores; transport at a much faster rate.
Cells carry out active transport in three main ways:
(1) Coupled transporters couple the uphill transport of one soluteacross the membrane to the downhill transport of another.
(2) ATP-driven pumps couple uphill transport to the hydrolysis of ATP.
(3) Light-driven pumps, found mainly in bacteria and archaea, couple uphill transport to an input of energy from light.
Transporters and active transport
Cells drive active transport in three ways
The actively transported molecule is shown in yellow and theenergy source is shown in red
- ion-driven transporters mediate secondary active transport- ATP-driven transporters mediate primary active transport
Three types of transporter-mediated transport
Uniports, symports, and antiports are used for both passiveand active transport
Coupled transport – the transport of one solute strictly depends on the transport of a second. It involves either the simultaneoustransfer of a second solute in the same direction (symporters) orthe transfer of a second solute in the opposite direction (antiporters)
Active transport
The tight coupling between the transport of the two solutes allowsthese carriers to harvest the energy stored in the electrochemical
gradient of one solute, typically an ion, to transport the other
The glucose-Na+ symport protein uses the electrochemical Na+
gradient to drive the import of glucose
The active transport of many sugars and amino acids into bacterialcells is driven by the electrochemical H+ gradient across the
plasma membrane
E. coli lactose permease
During the transport cycle, some of the helices undergo sliding motions causing them to tilt. An alternative opening and closing of the crevice between the helices exposes
the binding sites for lactose and H+, first on one side of the membrane and then the other
The Ca2+ pump
Structures of the unphosphorylated, Ca2+-bound state (left) and the phosphorylated, Ca2+-free state (right) have been determined by x-ray crystallography
Model showing how ATP binding and hydrolysis cause drastic conformational changes,bringing the nucleotide-binding and phosphorylation domains into close proximity. Thischange is thought to cause a 90° rotation of the activator domain, which leads to arearrangement of the transmembrane helices. The rearrangement of the helices disruptsthe Ca2+-binding cavity and releases the Ca2+ into the lumen of the SR.
The binding of cytosolic Na+ (1) and the subsequent phosphorylation by ATP of the cytosolic face of the pump (2) induce the protein to undergo a conformational change that transfers the Na+ across the membrane and releases it on the outside (3). The linkage of the phosphate to an
aspartic acid in the protein drives the conformational change. The binding of K+ on the extracellular surface (4) and the subsequent dephosphorylation (5) return the protein to its original conformation, which transfers the K+ across the membrane and releases it into the
cytosol (6). The changes in conformation are analogous to the A ⇌ B transitions of transporters, except that here the Na+-dependent phosphorylation and the K+-dependent
dephosphorylation of the protein cause the conformational transitions to occur in an orderly manner, enabling the protein to do useful work. For simplicity, only one Na+- and one K+-
binding site are shown. In the real pump in mammalian cells, there are three Na+- and two K+-binding sites. The net result of one cycle of the pump is therefore to transport three Na+ out
of the cell and two K+ in. Ouabain inhibits the pump by preventing K+ binding.
The Na+-K+ pump transports ions in a cyclic manner
Auxiliary transport system associated with transport ATPases in bacteria with double membranes
The transport ATPases belong to the ABC transporter supefamily
A typical ABC transporter consists of four domains – two highly hydrophobicdomains and two ATP-binding catalytic domains
ATP binding leads to dimerization of the two ATP-binding domains and ATPhydrolysis leads to their dissociation.
Nature Structural & Molecular Biology 11, 918 - 926 (2004)
The ATP switch model for the transport cycle of an ABC transporter
The schematic is for a drug exporter. The drug-binding site (red) is high-affinity and faces the inner leaflet of the membrane. Step I: the transport cycle is initiated by binding of substrate to its high-affinity site on the TMDs from the inner leaflet of the membrane. The affinity of the NBDs for ATP is increased, effectively lowering the activation energy for closed dimer formation. Two molecules of ATP bind, cooperatively, to generate the closed dimer. Step II: the closed NBD dimer induces a conformational change in the TMDs such that the drug-binding site is exposed extracellularly and its affinity is reduced, releasing the bound drug. Step III: ATP is hydrolyzed to form a transition-state intermediate. Hydrolysis of the two ATP molecules is normally sequential, although for some ABC transporters only one ATP may be hydrolyzed. Step IV: sequential release of Pi, and then ADP, restores the transporter to its basal configuration.
Ion channels
Can transport up to 100 million ions per second, a rate 105 timesgreater than that mediated by a carrier protein
Among their many functions, ion channels control the pace of the heart, regulate the secretion of hormones into the bloodstream, and generate the electrical impulses underlying information transfer in the nervous system.
Ion channels are ion-selective (ion selectivity) and fluctuatebetween open and closed states (gated)
Ion channels, like enzymes, have their specific substrates: potassium, sodium, calcium, and chloride channels permit only their namesake ions to diffuse through their pores. The ability of channels to discriminate among ions is called ion selectivity.
The atomic radius of K+ is 1.33 Å and that of Na+ is 0.95 Å
With only this difference to work with. K+ channels manageto select for the K+ ion over the Na+ ion by a factor of morethan 1000.
Ion Channel Gating
Ion channel gating refers to opening and closing of the ion conduction pore in response to a specific stimulus.
Certain channels open when ligands bind (ligand-gated channels); others open in response to membrane voltage
(voltage-gated channels); a few open in response to mechanicalstress (mechanically gated channels)
Converting sound and movement into electrical signals
a, The hair cell has an array of pencil-shaped stereocilia on its surface, each linked to its neighbour through a 'tip link'. b, The ion channel that mediates the conversion of sound or movement into electrical signals is located at one (and possibly both) ends of the tip link, which is shown here as a relatively stiff connection. The channel pore through which calcium (Ca2+) and potassium (K+) ions are transported is probably an assembly of four proteins, with TRPA1 as at least one of the subunits. The mechanism by which this channel is controlled is speculative, but it may involve a spring-like structural feature (with ankyrins forming the spring) and several other key proteins coupled into the actin core of the stereocilium.
Membrane potential – difference in electrical charge on the twosides of a membrane as a result of active electrogenic pumping
and passive ion diffusion
The equilibrium condition, in which there is no net flow of ionsacross the plasma membrane defines the
resting membrane potential
The Nernst equation expresses the equilibrium conditionquantitatively
The number of ions that move across the plasma membrane to set up the membrane potential is minute and the
movements of charge are generally rapid
Potassium channels are tetramers of identical subunits in which specific "signature sequence" amino acids are
responsible for potassium selectivity
The signature sequence is conserved in all potassium channels throughout nature and forms a structural unit
called the selectivity filter
K+ specificity of the selectivity filter in a K+ channel
In the vestibule, the ions are hydrated. In the selectivity filter, the carbonyl oxygens areplaced precisely to accommodate a dehydrated K+ ion. The dehydration of the K+ ionrequires energy, which is precisely balanced by the energy regained by the interactionof the ion with the carbonyl oxygens that serve as surrogate water molecules
EACH OF THE binding sites closely mimics potassium ions' octahedral hydration shell, thereby minimizing the energy required to strip off their water coats.
Because of their smaller size, sodium ions don't fit in these binding sites as snugly and thus find the energetic cost of trading their water coat for a spot in the selectivity filter too high.
From the x-ray structure of the KcsA potassium channel at 2.0-Å resolution it was determined that the selectivity filter
is a narrow, 12-Å-long segment of the pore that is lined with carbonyl oxygen atoms.
These atoms act as surrogate water molecules, allowing potassium ions to shed their hydration shell and enter into
the pore. Two potassium ions bind in the selectivity filter at once, usually with an intervening water molecule. Entry of a
potassium ion from one side of the filter is associated with potassium ion exit from the opposite side, resulting in rapid,
efficient potassium ion conduction. Electrostatic repulsion between ions apparently prevents the
potassium ions from binding too tightly, allowing high throughput in the setting of high selectivity.
A marvelous electrostatic feature of the potassium channel is a cavity of water half way across the membrane with
specifically oriented helices.
This design reduces the dielectric barrier, that is, the energetic cost of moving an ion from the high-dielectric
environment of water to the low-dielectric membrane, and thereby supports a high ion throughput.
Ion selectivityK+ channels specifically conduct K+ ions because the
selectivity filter contains multiple binding sites that mimic a hydrated K+ ion’s hydration shell.
Potassium channels achieve high conduction rates by exploitingelectrostatic repulsion between closely spaced ions and by
coupling the conformation of the selectivity filter to ion bindingwithin the filter
Gating
Large conformational changes within the membrane underliepore opening in K+ channels. Inner helices obstruct the pore
in the closed state and expand its intracellular diameter in the opened state.
Different gating domains – ligand binding and voltage sensing-appear to bring about a similar conformational change in the
pore of K+ channels
Voltage sensing
Conformational changes underlying voltage sensing in KvAPare large and involve movements of arginine residues through
the membrane, near the protein lipid interface
Conventional and novel view on opening a voltage-gated K+ channel(A) K+ channel subunit, showing the six transmembrane segments (S1–S6). Four (S1–S4) form voltage-sensor module with S4 as gating-charge carrier. S5 and S6 form rigid-pore module at centre of channel.(B) Conventional (upper panel) and MacKinnon view (lower panel) on opening. Pore region includes selectivity filter close to external surface, wide internal vestibule, and gate at internal surface. Conventional view assumes that S4 is located in oily interior of the protein and moves outward at positive internal potentials (helical screw model), causing gate to open. MacKinnon view assumes that S4 is connected with S3 and forms paddle that moves upward in lipid environment at positive potentials.
Aquaporins play a key role in cellular water homeostasis in humans, animals, and plants. These water channel proteins associate as tetramers. Each aquaporin contains a single 28-Å-long cylindrical pore that supports a string of nine hydrogen-bonded water molecules in single file.
Animations of the movement of water through aquaporins:
The protein backbone of one subunit of bovine aquaporin 1 is shown as a ribbon, with the dimensions of the water pore shown in blue dots. At right, water molecules (space-filling models) move through the pore single file. Residues defining the constriction region are shown as ball-and-stick representations. In particular, a histidine protruding into the pore ensures that molecules larger than water can't enter. (Gray = carbon, blue = nitrogen, red = oxygen.)
High-resolution structures reveal the inner workings of aquaporins in atomic detail
Water molecules travel single file through a narrow pore that connects water-filled intracellular and extracellular vestibules. This 28-Å-long pore is lined with a row of eight carbonyl oxygen atoms that can accept hydrogen bonds from the queue of water molecules, "ensuring that every water molecule is precisely oriented throughout the channel." But because the rest of the pore is lined with mostly hydrophobic amino acids, "water-pore interactions are kept to a minimum," allowing water to rush through each pore at a rate of 3 x 109 molecules per second.
FOR A SHORT STRETCH, the pore narrows to just wide enough for a water molecule to pass, preventing larger molecules from entering.
And ions--which would have to abandon their bulky water coats to enter--don't do so because the pore isn't a good substitute. In addition, a conserved, positively charged arginine residue in this region prevents cations, including protonated water (H3O+), from entering.
Nature has gone to great effort to prevent protons from passing through, too. Previous structural work had suggested that a pair of asparagine residues as well as the dipole moments of short -helices would force water molecules to flip near the pore's center. This forced reorientation breaks the continuous chain of hydrogen-bonded waters and prevents protons from "hopping" along the hydrogen-bond network.
PROTON BLOCKSimulation of water molecules passing through an aquaporin suggested that the central water molecule is forced out of line in part by hydrogen bonds donated by a pair of asparagines and this defect in the hydrogen-bonded water wire caused the exclusion of protons in these channels. Recent simulations of protons passing through the protein have called this mechanism into question.
Normally, continuous, single-file columns of water like these conduct protons.
Protons can easily hop along a similar single-file water column in the water pore of gramicidin A. But unlike gramicidin A, an antibiotic peptide that kills bacteria by forming pores in their cell membranes, aquaporins must not leak protons. Doing so would disturb the delicate balance of charge
across cell membranes that underlies cellular function.
So how do aquaporins manage to do what gramicidin A can't?
The water column in aquaporins does have some distinguishing features. Near the center of the aquaporin pore, hydrogen bonds from a pair of asparagine residues (as well as the pull of nearby -helix dipoles) reorient the central water molecule, preventing it from accepting a proton from nearby water molecules.
From structural observations, it was suggested that the forced reorientation of this central water molecule might break the continuous chain of hydrogen-bonded waters and possibly prevent protons from "hopping" along the hydrogen-bonded water "wire."
The barrier to proton transfer is highest in the part of the aquaporin pore that boasts the highest electrostatic potential: the region centered around the two central asparagines and the partially positively charged -helix dipoles, known as the NPA region.
Recent results indicate that the main barrier to proton transfer is not caused by interruption of the hydrogen-bonded water chain, as had previously been speculated, but rather by an electrostatic field created by the -helix dipoles in the NPA region.
The barrier to proton transfer in aquaporins is estimated to be 6–7 kcal per mole--about the same as estimates of the barrier to proton translocation across lipid membranes
Voltage-gated cation channels generate action potentials inelectrically excitable cells
- neurons- muscle cells- endocrine cells- egg cells
An action potential is triggered by a depolarization of the plasma membrane – thatis, by a shift in the membrane potential to a less negative value.
Propagation of an action potential along an axon without attenuation
Action potentials are the direct consequence of the properties ofvoltage-gated cation channels
Fluorescence micrograph and diagram of individual myelinated axons teased apart in a nerve. Three different proteins are detected – voltage-gated Na+
channels stained in green are concentrated in the axonal membrane at thenodes of Ranvier; an extracellular protein called Caspr, stained in red, marksthe end of each myelin sheath; voltage-gated K+ channels, stained in blue, localize to regions in the axon plasma membrane close to the nodes
Chemical synapses can be excitatory or inhibitory
Excitatory neurotransmitters open cation channels, causing an influx of Na+ that depolarizes thepostsynaptic membrane toward the threshold potential for firing an action potential.
Inhibitory neurotransmitters open either Cl- channels or K+ channels, and this suppresses firing bymaking it harder for excitatory influences to depolarize the postsynaptic membrane.
The acetylcholine receptors at the neuromuscular junction aretransmitter-gated cation channels
Three conformations of the acetylcholine receptor