CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small...
-
Upload
dulcie-barton -
Category
Documents
-
view
217 -
download
0
Transcript of CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small...
![Page 1: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/1.jpg)
CHAPTER 1: Some Tools of the Trade
Lab 1.1
2014
![Page 2: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/2.jpg)
Purpose of Lab 1.1
• Become familiar with the small volumes of solutions used in molecular biology
• Introduce proper use of the micropipette
• Practice using the micropipette
![Page 3: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/3.jpg)
Micropipettes
![Page 4: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/4.jpg)
Parts of the micropipette
![Page 5: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/5.jpg)
Micropipette use
1. Twist dial to desired volume2. Add disposable pipette tip3. Press plunger to first stop4. To retrieve liquid, slowly release plunger5. To transfer liquid, press plunger past first
stop to second stop6. Keep plunger down as you remove pipette7. Eject tip
![Page 6: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/6.jpg)
Reading a P-20 micropipette
![Page 7: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/7.jpg)
Reading a micropipette (cont.)
0
5
0
0
5
0
0
5
0
5 μL 50 μL 500 μL
P-20 P-200 P-2000
![Page 8: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/8.jpg)
Micropipetting “nevers”
• Never use micropipette without a tip
• Never lay micropipette down with sample in the tip
• Never let the plunger button snap back
• Never set the P-20 pipette below 2.0μL or above 20.0μL
![Page 9: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/9.jpg)
Pipetting techniques
Hold micropipette and microfuge tubes at eye level when loading or dispensing samples
![Page 10: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/10.jpg)
Pipetting techniques (cont.)
Place both elbows on the table and use your other hand to stabilize the bottom of the pipette
![Page 11: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/11.jpg)
Different amounts
The amount you push the plunger depends on the amount: At 20.0μL the plunger is much higher than at 2.0μL
![Page 12: CHAPTER 1: Some Tools of the Trade Lab 1.1 2014. Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.](https://reader035.fdocuments.in/reader035/viewer/2022081515/56649f355503460f94c53af0/html5/thumbnails/12.jpg)
Practice Pipetting Sheet
20 μL
0
0
0
0
15 μL
0
0
0
0
10 μL
0
0
0
0
5 μL
0
0
0
0
2 μL
0
0
0
0