Center for Homogeneous DNA Analysis
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Transcript of Center for Homogeneous DNA Analysis
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Center for Homogeneous DNA Analysis
University of UtahDepartments of Pathology, Mathematics, and
Engineering
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What We Do And Why
• High Resolution Melting Analysis– We Watch DNA Unwind Very Carefully
• Mutation Scanning, Genotyping, Transplant Compatibility
• Proprietary Technology
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What’s So Special?
• Faster, More Economical – Minutes vs. Hours– Pennies vs. Dollars
• We Do It Automatically Without Opening The PCR Tube - Simpler
• Highly Accurate – Detects Single Base Changes In 1000bps
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High-Resolution Melting Analysis: Hardware, Reagents, Software
CCD Camera
LEDs
ExcitationFilter
MicrotiterPlate
Emmision Filter
LightScanner™
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A Medically Important Mutation - 3000 tests/mo in UT
Temperature
“Auto Call” of Plate Using Unbiased Hierarchal Clustering
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Transplant Matching
CEPH Family UT1331
3, 5, 6, 11, 12
2, 9, 10, 13
8
1, 4, 7
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Transplant Matching
• Traditionally done by sequencing or serology:– $1,200 per family member ($15,600 for UT1331
family)• High-resolution melting analysis:
– can be priced as low as $100 per family member ($1,300)
• Transplants: 50,000/year U.S.
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Current Status• Scanning Technology
– Partly Licensed to Utah Company– Product Sales: $600,000 first year– Enthusiastic customer feedback (e.g. Mayo
Clinic)– Twelve new jobs
• Two Fast Track STTRs granted– $1.7M matching funds– For advanced instrumentation
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• Make DNA screening routine for:– Research (individual labs ~ core labs)– Gene-mapping organizations– Clinical reference labs– Eventually, hospital STAT labs
• Displace 95% of DNA sequencing market
The Vision
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Market Size & Forecast• 42 million assays annually
– $10M using melting analysis– Displaces $150M using sequencing
• 12,000 instruments, software, $195M
• Reagents• Developing market• HLA, 50,000 transplants (US) • Molecular tests growing at +15%
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• DNA diagnosis at your doctor’s office.– PCR in 15 min– Melting in 2 min– Immediate automated analysis
Future Reality
PCR and melting on a chip1 32 384 384,000
Whole genome analysis (30,000 genes)