Cellular Respiration Investigation

Question

How does temperature affect the rate of respiration in germinating

and non-germinating peas?

C6H12O6 + 6O6 6CO2 + 6H2O + 686 kcal of energy/mole of glucose

How could the rate of cellular respiration be measured?

Measuring Oxygen Consumption

General Gas Law

PV = nRTP = Pressure of the gasV = Volume of the gasn = number of molecules of gasR = gas constantT = temperature of the gas (in ⁰K)

If P and T are constant?

If V and T are constant?

If n and T are constant?

If T changes and n is constant?

Measuring Oxygen Consumption

Removing Carbon Dioxide

CO2 + 2KOH K2CO3 + H2O

*Since any carbon dioxide produced will be removed, any change in the volume of gas will be directly related to changes in oxygen.

Apparatus Used

Respirometer Trials

Respirometer Temperature Contents

1 Room Germinating Seeds

2 Room Dry Seeds + Beads

3 Room Beads

4 10⁰C Germinating Seeds

5 10⁰C Dry Seeds + Beads

6 10⁰C Beads

Prediction and Hypothesis

Record in your notebook…

Procedure

Record Procedure and Data Collection Table inyour notebook as a pre-lab entry.

Analysis

• Make a graph of your data. Show each variable as one line.

• Describe the data.• Discuss errors.

- What were they?- How did they affect the data?- How could they be corrected?

Conclusion

• Compare actual to expected results.• EXPLAIN the results for each variable. Use your

knowledge of the equation for respiration and the Gas Law.-Explain why water moves in a respirometer’s pipette.-Compare the amounts of oxygen consumed by germinating corn seeds at room temperature and in the ice bath.-Compare the results for dry and germinating seeds. -What are some processes in plants that require ATP?

• Further Questions• Real-life application