CELL-tainer® Single use Bioreactor

November 2013

CellD20bis, rue du Chapitre F-30150 ROQUEMAURETel : +33 (0)4 66 82 82 60Fax : +33 (0)4 66 90 21 10Email : contact-info@celld.com www.celld.com

CELL-tainer®

A new single-use bioreactor with two-dimensional movement that offers superior mass transfer rates and short mixing times with low shear for high density cell culture and microbial fermentation

2

Features

MASS-transfer & mixing

Two dimensional rocking improves mixing and oxygen transfer

Proper and accurate temperature control

Heating by convection (incubator)

Direct cooling (heat exchanger under the bag)

Short mixing times => no gradients

EASY handling & real single use

Bags flexible in volume

Robust design (stackable)

In-line measurements non-invasive,

fully disposable (pH, DO,), bottom mounted,

others (glucose/lactate)

Scaleable

Same process performance at different scales

Complete system Incubator chamberIntegrated process controls with HMI touchscreen21 CFR part 11 compliantPLC controller for rpm, pH, DO, Temp3 x Gas flow metersPressure gauges2 x Addition pumpsHeavy duty motorRemovable bag tray

Features

Specifications

CELL-tainer® single-use bag

Microbial bag

Benefits

Pre-sterilized and disposable

Reduce handling

Plug and play operation

Quick change over

Expansion in a single bag

=

Minimize contamination risk

Reduce variability

Save time and labor

Cycle time reduction

Cost effective

7

2-dimensional movement

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The kla-value determined with the dynamic gassing in/out method including the off-gas analysis.

At the 20 L CELL-tainer, optimal filling volumes are above 8L, rocking speeds above 25 rpm.

At the 200 L CELL-tainer, the optimal filling volume ranges between 90 and 130L. At rocking speeds between 15 and 25 rpm, highest kla-values of 400 h-1 are obtained.

0

100

200

300

400

500

600

15

20

25

30

35

68

1012

14

kLa

[h-1

]

rpm

Liquid volume [L]

0

100

200

300

400

500

600

10

15

20

25

30

8090

100110

120130

140150

kla-value at different rocking speeds

12 L

120 L

Cultivation of E. coli (Fed-batch):

Final cell dry mass concentration of 45 gL-1 (OD=140) obtained in 35 h.

(Junne et al, ChemieIngenieurTechnik Vol. 85 2013)

Cultivation results

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Batch and Fed-batch E.coli

20L CELL-tainer®  

CELL-tainer® scale up

CONFIDENTIAL

200L CELL-tainer®

CELL-tainer® 200L single-use bag

Mammalian: 5L – 200L working volume

Microbial: 5L – 120L working volume

CONFIDENTIAL

Service Support

CONFIDENTIAL 16

1 Year Manufacturer’s Warranty-Covers mechanical and electronic parts not subject to wear and tear (excluding labor and travel)

Service support-24 hour response with phone/email support to troubleshoot and diagnose problem-If issue is not resolved, an engineer will schedule on-site visit for servicing

Anton Tromper - anton.tromper@cellutionbiotech.com

Ruben Engelhart - ruben.engelhart@cellutionbiotech.com

Lexan Lhu – llhu@lydall.com

Spare parts-Stocked at Charter Medical and available to ship FedEx next day

Optional add-on services / products-Maintenance contract - -Site Acceptance Test (at customer site) - one day for CT20 and 2 days for CT200, excluding travel-Operational training (at customer site) - one day for CT20 and 2 days for CT200, excluding travel-CELL-tainer DACS software for one CELL-tainer including 1 Modbus license -CELL-tainer DACS software for one CELL-tainer including 1 Modbus license -Modbus license for connecting additional CELL-tainer (up to 8) -HMI license for connecting additional CELL-tainer (up to 8)

THANK YOUFor More Information: www.CharterMedical.com

Contact Info:Dominique Ghozlandominique.ghozlan@celld.com04 66 82 82 60

CellD20bis, rue du Chapitre F-30150 ROQUEMAURETel : +33 (0)4 66 82 82 60Fax : +33 (0)4 66 90 21 10Email : contact-info@celld.com www.celld.com