Cell Structure and Function. Principles of Cell Theory Unit of life All living things are made of...
Transcript of Cell Structure and Function. Principles of Cell Theory Unit of life All living things are made of...
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Cell Structure and Function
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Principles of Cell Theory
• Unit of life
• All living things are made of cells (Schwann and Schleiden)
• All cells come from cells (no spontaneous generation -Virchow)
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Cells
• unicellular• Multicellular
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cells• Many types• Different functions
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Discovery of Cells
• Robert Hooke (mid-1600s)– Observed sliver of cork– Saw “row of empty boxes”– Coined the term cell
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Cell size
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Characteristics of All Cells
• Membrane around cytoplasm
• Cytoplasm – thick fluid with organelles
• Organelles – “little organs”
• DNA
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Organelles
• Cellular “organs”
• Two general kinds– Derived from membranes– Bacteria-like organelles
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Bacteria-Like Organelles• Endosymbiotic theory:
– Ancient association between symbiotic bacteria and primitive cells
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Cell Types
• Prokaryotic: bacteria
• Eukaryotic: plants, animals
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Prokaryotic Cells• No nucleus – has a “nucleoid” with DNA• Organelles without membranes• First cell type on earth• Smaller than eukaryotic cells• bacteria
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Eukaryotic Cells• Nucleus with a membrane
• Possess many organelles with membranes
• Plants, Animals, fungi, protozoans
Protozoan
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Eukaryotic vs. prokaryotic cells
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EukaryoticPlant vs animal cells
Centriolesribosomes
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Representative Animal Cell
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Representative Plant Cell
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Plasma Membrane
• Contains cell contents
• Double layer of phospholipids & proteins
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Phospholipids
• Polar– Hydrophylic head– Hydrophobic tail
• Interacts with water
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Movement Across the Plasma Membrane
• A few molecules move freely– Water, Carbon dioxide, Ammonia, Oxygen
• Carrier proteins transport some molecules– Proteins embedded in lipid bilayer– Fluid mosaic model – describes fluid nature of
a lipid bilayer with proteins
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Membrane Proteins
1. Channels or transporters– Move molecules in one direction
2. Receptors – Recognize certain chemicals
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Membrane Proteins
3. Glycoproteins – Identify cell type
4. Enzymes – Catalyze production of substances
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Cell Walls
• Found in plants, fungi, & many protists
• Surrounds plasma membrane
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Cell Wall Differences
• Plants – mostly cellulose
• Fungi – contain chitin
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Cytoplasm• Viscous fluid containing organelles• components of cytoplasm
– Interconnected filaments & fibers – Fluid = cytosol– Organelles (not nucleus)– storage substances
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Cytoskeleton• Filaments & fibers
• Made of 3 fiber types– Microfilaments– Microtubules– Intermediate filaments
• 3 functions:– mechanical support– anchor organelles– help move
substances
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A = actin, IF = intermediate filament, MT = microtubule
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Cilia & Flagella
• Provide motility• Cilia
– Short– Used to move substances
outside human cells
• Flagella – Whip-like extensions– Found on sperm cells
• Basal bodies like centrioles
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Cilia & Flagella Structure
• Bundles of microtubules
• With plasma membrane
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Centrioles
• Pairs of microtubular structures
• Play a role in cell division
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Membranous Organelles
• Functional components within cytoplasm
• Bound by membranes
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Nucleus
• Control center of cell
• Double membrane
• Contains – Chromosomes– Nucleolus
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Nuclear Envelope
• Separates nucleus from rest of cell
• Double membrane
• Has pores
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DNA
• Hereditary material
• Chromosomes– DNA– Protiens– Form for cell division
• Chromatin
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Nucleolus
• Most cells have 2 or more
• Directs synthesis of RNA
• Forms ribosomes
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Endoplasmic Reticulum
• Helps move substances within cells
• Network of interconnected membranes
• Two types– Rough endoplasmic reticulum– Smooth endoplasmic reticulum
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Rough Endoplasmic Reticulum
• Ribosomes attached to surface– Manufacture protiens– Not all ribosomes attached to rough ER
• May modify proteins from ribosomes
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Smooth Endoplasmic Reticulum
• No attached ribosomes
• Has enzymes that help build molecules– Carbohydrates– Lipids
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Golgi Apparatus
• Involved in synthesis of plant cell wall
• Packaging & shipping station of cell
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Golgi Apparatus Function
1. Molecules come in vesicles
2. Vesicles fuse with Golgi membrane
3. Molecules may be modified by Golgi
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Golgi Apparatus Function (Continued)
4. Molecules pinched-off in separate vesicle
5. Vesicle leaves Golgi apparatus
6. Vesicles may combine with plasma membrane to secrete contents
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Lysosomes
• Contain digestive enzymes• Functions
– Aid in cell renewal– Break down old cell parts – Digests invaders
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Vacuoles
• Membrane bound storage sacs
• More common in plants than animals
• Contents – Water– Food– wastes
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Bacteria-Like Organelles
• Release & store energy
• Types – Mitochondria
(release energy) – Chloroplasts
(store energy)
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Mitochondria
• Have their own DNA
• Bound by double membrane
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Mitochondria
• Break down fuel molecules (cellular respiration)
– Glucose– Fatty acids
• Release energy– ATP
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Chloroplasts
• Derived form photosynthetic bacteria
• Solar energy capturing organelle
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Photosynthesis
• Takes place in the chloroplast
• Makes cellular food – glucose
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Review of Eukaryotic Cells
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Review of Eukaryotic Cells
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Molecule Movement & Cells
• Passive Transport
• Active Transport
• Endocytosis
(phagocytosis & pinocytosis)
• Exocytosis
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Passive Transport
• No energy required
• Move due to gradient– differences in concentration, pressure, charge
• Move to equalize gradient– High moves toward low
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Types of Passive Transport
1. Diffusion
2. Osmosis
3. Facilitated diffusion
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Diffusion
• Molecules move to equalize concentration
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Osmosis
• Special form of diffusion
• Fluid flows from lower solute concentration
• Often involves movement of water– Into cell– Out of cell
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Solution Differences & Cells• solvent + solute = solution
• Hypotonic– Solutes in cell more than outside– Outside solvent will flow into cell
• Isotonic– Solutes equal inside & out of cell
• Hypertonic– Solutes greater outside cell– Fluid will flow out of cell
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Facilitated Diffusion
• Differentially permeable membrane
• Channels (are specific) help molecule or ions enter or leave the cell
• Channels usually are transport proteins (aquaporins facilitate the movement of
water)• No energy is used
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Process of Facilitated Transport
• Protein binds with molecule
• Shape of protein changes
• Molecule moves across membrane
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Active Transport
• Molecular movement
• Requires energy (against gradient)
• Example is sodium-potassium pump
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Endocytosis
• Movement of large material– Particles– Organisms – Large molecules
• Movement is into cells
• Types of endocytosis– bulk-phase (nonspecific)– receptor-mediated (specific)
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Process of Endocytosis
• Plasma membrane surrounds material
• Edges of membrane meet
• Membranes fuse to form vesicle
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Forms of Endocytosis
• Phagocytosis – cell eating
• Pinocytosis – cell drinking
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Exocytosis
• Reverse of endocytosis
• Cell discharges material
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Exocytosis
• Vesicle moves to cell surface
• Membrane of vesicle fuses
• Materials expelled
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End Chapter 5