Cell Storage Best Practices: Techniques to Ensure Greater Research Results
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Your Reliable Source of Human Primary Cells Cell Storage Best Practices: Techniques to Ensure Greater Research Results AllCells Exclusive Webinar Event 9/17/2015 12pm PST Featuring Mike George, Cryopreservation Specialist
Transcript of Cell Storage Best Practices: Techniques to Ensure Greater Research Results
Your Reliable Source of Human Primary Cells
Cell Storage Best Practices:Techniques to Ensure Greater Research Results
AllCells Exclusive Webinar Event 9/17/2015 12pm PST
Featuring Mike George, Cryopreservation Specialist
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Outline
•Introduction•Life science storage methods•Comparison of equipment•What not to do (common mistakes/misconceptions)•Discussion with James Lee – Storage of Primary Cells•Q&A
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Introduction
•Mike George, Cryopreservation Specialist• Owner of MG Lab Solutions Inc• 17 years experience specifying, selling and
servicing cryogenic equipment• Other equipment to meet laboratory and storage
needs
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Background on storage methods•Material can be stored in -80c, liquid nitrogen vapor or liquid nitrogen liquid•Determine your temperature requirements based on the material to be stored•Will this be short term or long term storage?•Glass Transition Temperature
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Comparison of storage equipment
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Comparison of storage equipment
•Select your storage vessel and inventory system•Personal Protective Equipment (PPE)•Ultra-Low (-80c) Storage•Liquid Nitrogen Storage – Liquid Phase•Liquid Nitrogen Storage – Vapor Phase•How to prepare your samples for storage•Cost of Equipment
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What not to do
•Ignore maintenance •Do not plan ahead for inventory safeguards i.e., data tracking, temperature monitoring, storage tools and safety training•Assume all is fine – check temperatures, viability and content
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Storage of human primary cells•James Lee, Associate Director Production, AllCells• 16 years primary cells experience• Specializing in cell isolation, culture
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Storage of human primary cells•Special considerations for storage of human primary cells vs cell lines, etc? •Collection•Processing•Freezing down at control rate•Work quickly
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Storage of human primary cells
•Process and equipment setup at AllCells lab•Presentation of AllCells tank data
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Storage of human primary cells
•Cryo media discussion•Different solutions•AllCells VitalCellsTM
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Transportation of human primary cells•Transport vessel options for shipment of cells:• Dry ice/liquid nitrogen dry shippers
•International, long distance shipments•Timing considerations • Domestic• International
•Courier options
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Q&A Please use the prompt on the right of your
screen to send a question to the organizer
Questions will be read and answered
Any offline questions may be sent to [email protected]
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Mike George
Cryopreservation Specialist, MG Lab Solutions
Please direct offline questions to [email protected]
510-610-3656
James Lee
Associate Director of Production, AllCells
Please direct offline questions to [email protected] or [email protected]
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information
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Following Webinar
Email to follow with information on how to obtain:•a free sample bottle of AllCells VitalCells™ Cryo Media solution•a free vial of our high quality Frozen PBMC product•an exclusive 10% discount coupon eligible for a future purchase of any of our popular Frozen PBMC products: PB003F, PB004F, PB005F, PB006F
