Cell Disruption Solutions Mini Brochure... · 2012. 10. 24. · 40 KPSI (2700 bar) 30 KPSI (2000...

cell disruption

protein extraction

enzyme release

continuous processing

DNA manipulation

single cell isolation

tissue disruption

selective breakage

Constant Systems Research Models

Cell Disruption Solutions

Cell Disruption it’s in our Genes

Taking your disruption out of Cell Disruption

ISO 9001:2000 RegisteredFS67550

www.constantsystems.com

Rev 0

Principles of Operationof

Constant Cell Disruption Systems are based on the use of high pressure to force a sample through a small fixed orifice at high speed under controlled, contained and repeatable conditions.

As the high pressure piston descends, sample is introduced into the high pressure cylinder.

1.

The high pressure piston then accelerates the sample through the fixed orifice at high velocity. The controlled acceleration causes the cells to rupture.

2.

When the jet containing disrupted cells hits the target, it is spread radially across the cooled heat exchange surface. Product then flows from the disruption chamber for collection. The hydraulics are recharged and the cycle continues.

3.

Reproducible ResultsConstant Cell Disruption Systems give reproducible and superior results because control of the disruption pressure is achieved by the hydraulic control system.

The disruption pressure cycle is shown adjacent. This has been created by analysing and recording the hydraulic pressure in the operating cylinder, which is related to the disruption pressure by the ratio of the piston areas.

Contained ProcessThe disruption head, high pressure cylinder and cooling system have been designed with containment and safety in mind so that the volume of high pressure product is kept to minimum at all times. The integrity of the containment system has been tested by the Biosafety Testing Station at the Centre for Applied Microbiology Research, UK. “ ...a high level of bio-containment.” “No evidence of emission from the equipment in any test.”

2

Graph showing a typical controlled pressure cycle in the cell disrupter

Disruptionstroke

Returnstroke

Recharging period for hydraulics

30

20

10

40

30

20

10Pre

ssur

e (b

ar x

100

) Pressure (psi x 1000)

2 4 6 8 10 12 14Time (second)


Specifications shown over leaf

Specifications shown overleaf

The One Shot model is the ideal research tool for small processing. It can process solid samples, cell paste and suspended samples quickly and efficiently. Work and results can be scaled up throughout the range.


Power Rating Volume per Shot vs Maximum Pressure

40 KPSI (2700 bar) 30 KPSI (2000 bar) 20 KPSI (1350 bar) 15 KPSI (1000 bar)

0.75 KW 1-8ml 1.5-10.5ml 2-16ml 3-20.5ml

One Shot Model


One Shot Model

Constant Systems Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

450

475

Incorporates our unique disruption head and precise hydraulic operating and control system

Quick and easy to use

Disruption pressure easily and accurately set and displayed digitally with a range of1KPSI through to 40KPSI as standard

Ultra low loss

Contained during operation

Pressure consistent and stable during disruption cycle

Disrupter head mounted in stainless steel tray to avoid spillage

Easily dismantled for manual cleaning and autoclaving

Allows loading of solid sample, cell paste or suspended sample

Collection cups provided can be transferred to and from ice or a freezer for

Materials in contact with sample, 316L and F51 duplex stainless steel or equivalent, synthetic sapphire, EPDM, GLFPTFE & PEEK 450G


Specifications shown overleaf

The TS 0.75kw model is the ideal research/pilot tool for processing between 40mls and 200mls and is versatile to adapt as your needs change.


Power Rating Flow Rates vs Maximum Pressure


0.75 KW 40ml/min 55ml/min 80ml/min 115ml/min

TS Series 0.75kw Model


TS Series 0.75kw Model

Incorporates our unique disruption head and precise hydraulic operating control

system Disruption pressure easily and accurately set and displayed digitally with a

range of 1KPSI through to 40KPSI as standard

Touch screen control of disruption cycle, automatic shut down when process

complete

Disruption head mounted on tray to contain accidental spillages

Partial containment and simple externally controlled CIP

Internal relief valve fitted for reverse flow of cleaning fluids

Various flow rates are available depending on desired maximum pressure and power

rating, alternative pressure heads available on request


Full bio-containment inlet and outlet options available upon request

Materials in contact with sample, 316L and F51 duplex stainless steel or equivalent,

synthetic sapphire, EPDM, GLFPTFE & PEEK 450G

Temperature Control: Disruption head is surrounded by a unique cooling jacket. Bath circulator or chiller is recommended.


750

690


TS Series Cabinets are the ideal pilot production models and are versatile enough to adapt as your needs change.


TS Series Cabinet



2.2 KW

4 KW

190ml/min

405ml/min

240ml/min

430ml/min

300ml/min

440ml/min

310ml/min

565ml/min


TS Series Cabinet

Incorporates our unique disruption head and precise hydraulic operating control system

Disruption pressure easily and accurately set and displayed digitally with a range of 1KPSI through to 40KPSI as standard

Touch screen control of disruption cycle, automatic shut down when process

complete

Cell Disrupter controls peristaltic process pump for automatic supply of larger

samples.Process pump available on request

Disruption head mounted in stainless steel tray to avoid spillage

Partial containment and simple externally controlled CIP

Internal relief valve fitted for reverse flow of cleaning fluids

Various flow rates are available depending on desired maximum pressure and power rating, alternative pressure heads available on request


Full bio-containment inlet and outlet options available on request

Materials in contact with sample, 316L and F51 duplex stainless steel or equivalent,

synthetic sapphire, EPDM, GLFPTFE & PEEK 450G

IQ/OQ available on request


Temperature Control: Disruption head is surrounded by a unique cooling jacket . Bath circulator or chiller is recommended.


The B & C Series are the ultimate industry cell disrupters, with the power and processing ability to consistently disrupt the materials that you require.




1.1 KW

2.2 KW

100ml/min 120ml/min 180ml/min 255ml/min

B & C Series

4 KW




B & C Series

Incorporates our unique disruption head and precise hydraulic operating control systemwith CIP and SIP capability

Disruption pressure easily and accurately set and displayed digitally with a range of 1KPSI through to 40KPSI as standard

Cell Disrupter controls peristaltic process pump for automatic supply of larger samples.Process pump available on request

Fully contained and having internal ports and drain ports to allow CIP and SIP

The “B Series” allows the CIP and SIP to be manually operated

The “C Series” includes all valves which can be tailored to customers requirements and validated

Materials in contact with sample, 316L and F51 duplex stainless steel or equivalent, synthetic sapphire, EPDM, GLFPTFE & PEEK 450G

IQ/OQ available on request

Various flow rates are available depending on maximum pressure and power rating, alternative pressure heads available on request


Constant System Limited, Low March, Daventry, Northants, NN11 4SD, United KingdomTelephone: + 44 (0) 1327 314146 Fax : + 44 (0) 1327 314147

Validation: Industry standard validations available on request

690

785

*B Series Bench Top model shown

above and B series Cabinet model

shown on the reverse

Constant Cell Disrupter Systems are extremely versatile and have been used for the following range of application:

Cell Disruption, Protein & DNA Extraction, Enzyme Release, DNA Manipulation, Selective Breakage, Single Cell Isolation, Tissue Disruption

Individual Organisms and samples processed by Constant Cell Disrupter Systems are too numerous to mention them all, however the following lists examples:

Algae Disruption of anabaena variableAnimal Cells Selective breakage of chicken spermBacteria Good enzyme activity from recombinant e.coli. High protein recovery from mycobacterium smegmatis

Fungi High specific activity of enzyme from aspergillus nidulans. 99% protein release(inc Yeasts) from saccharomyces cerevisiae. 99% Recombinant protein release from pichia

pastoris

Mammalian Disruption of cellular and intracellular plasma membranes from horse liver IsolationCells and Tissue of viable single cells from calf spleen and lymph node

Parasites Breakage of cytomegalovirus in nucleus of human cells

Plant Cells Pectin extraction from banana fruit. Strawberry leaf DNA extraction& Tissue

Viruses Isolation of membrane antigens from toxoplasma gondi. Separation of nucleus and membrane in amniotic cells

Environmental Extraction of DNA from soil bacteriaSamples

E.coli Disruption & Guidance List

Constant Systems Cell Disrupters experience with E.coli is vast and breakage depends on the cell culture conditions, length of growth cycle an dpressure utilised.We state the following as a guide to users for pressure compared to breakage with E.coli:

Release of DNA – Below 15,000 psi (1000 Bar)

Cytoplasmic Protein – 15,000 psi – 20,000 psi (1350 Bar)

Inclusion Bodies – 20,000 psi – 27,000 psi (1900 Bar)

Membrane Protein – 27,000 psi – 35,000 psi (2400 Bar)

Yeast Disruption Guide

Please find below a list of some of the other more common species of yeast with pressure vs. breakage:

·Candida Cloacae - 40 Kpsi·Candida Albicans - 25 - 40 Kpsi·Candida Utilis - 15 - 40 Kpsi·Hansenula Polymorpha - 25 - 40 Kpsi·Pichia Augusta - 40 Kpsi·Pichia Capsulatus - 40 Kpsi·Pichia Picorna - 25 - 35 Kpsi·Pichia Pastoris - 35 - 40 Kpsi·Kluyveromyces Fragilis - 40 Kpsi·Rhodotorula Glutinis - 40 Kpsi·Schizosaccharomyces Pombe - 15 - 40 Kpsi·Saccharomyces Cerevisiae - 27 - 35 Kpsi

Your Application and Cell Success

Cell Disruption Solutions Mini Brochure... · 2012. 10. 24. · 40 KPSI (2700 bar) 30 KPSI (2000...

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Transcript of Cell Disruption Solutions Mini Brochure... · 2012. 10. 24. · 40 KPSI (2700 bar) 30 KPSI (2000...