Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage:...

51
Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 S: 24/25 hygroscopic g Comment: Composition Powder media from AppliChem are mixed and processed according to the original formulations. To produce these media only raw materials of high quality are used. The single raw material that is used in the media production is taken only from one lot, which is dried according to instructions. Shortly, after drying the raw materials, they will be grinded in special mills to get a homogenous fine powder, that is packed immediately. Due to that the customer can easily dissolve our media. You will receive a weighing protocol on request. HEPES-buffered media HEPES-buffered media are often used, whenever the pH-value has to be stable in the media. With the addition of HEPES the pH value will get an additional stability in the pH range 7.2 to 7.6 for pH-sensitive cell culture systems. Deviations of the pH value in the media are caused by the metabolism of the cultivated cells but also by changes of the CO2 concentration during incubation. Storage All powder media are produced without sodium hydrogen carbonate to increase the stability. Keeping them tightly closed is absolutely necessary as they are very hygroscopic. For this reason one package of powder media should always be dissolved completely. Storing dry and cold (+4 to +8˚C) will increase the shelf life, which is normally two years. Generally the time for delivery will be one week. Customer formulations As a special service AppliChem offers the production of powder media according to the customer formulation for competitive prices - even in smaller quantities. In our laboratories we are able to consider most wishes regarding the composition of the powder media. The minimum order size is 20 liters with a delivery-date of two to three weeks, generally. Please ask for our offer for your formulation giving us the exact composition and the quantity you need. Literature BME: Eagle, H. (1965) Proc. Soc. Exp. Biol. Med. 89, 362 DMEM: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 DMEM/F12: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 Barnes, D. & Sato, G. (1980) Anal. Biochem. 102, 255 Earle's buffered salt mixture: Earle, W. (1943) J. Nat. Cancer Inst.4, 165 Gamborg's B5 - Plant salts: Gamborg O.L., Miller R.A., OjimaK. (1968) Exp. Cell Res. 50,151 Glasgow - MEM (BHK 21): MacPherson, I. & Stoker, M. (1961) Virology 16, 147 Grace's - Insect medium: Grace, T.D.C. (1962) Nature 195, 788 Grace, T.D.C. (1962) Nature216, 613 Grace, T.D.C. (1966) Nature 211, 366 Ham's F10 - Medium: Ham, R.G. (1963) Exp. Cell Res. 29, 515 Ham's F12K - Medium: Kaighn, M.E., (1973) Tissue Culture Methods and Applications , 54 (eds. Kruse & Patterson) Ham's F12 - Medium: Ham, R.G. (1965) Proc. Nat. Acad. Sci USA 53, 288 Hanks' buffered salt mixture: Hanks', J.H. & Wallace, R.E. (1949) Proc. Soc. Exp. Biol. Med. 71, 196. Iscove's mod. Dulbecco's MEM (IMDM): Iscove, N., N. & Melchers, F (1978) J. Exp. Med. 147, 926 Iscove, N., N. et al. (1980) Exp. Cell Res. 126, 121 IPL-41 - Medium: Weiss, S.A., G.C. Smith, S.S. Kalter & J.L. Vaughn. (1981) In Vitro 17, 495. Joklik - MEM: Eagle, H. (1959) Science 130, 432 Leibovitz's L15 - Medium: Leibovitz, A. (1963) Amer. J. Hyg. 78, 173 Linsmaier, Bednar & Skoog - Plant salts: Linsmaier E.M. & Skoog F. (1965) Physiol. Plantarum 18, 100 McCoy's 5A - Medium (modified): McCoy, T.A. et al. (1959) Proc. Soc. Exp. Biol. Med. 100, 115 Hsu, T.C. & Kellogg, D.S. (1960) J. Nat. Cancer Inst. ,25, 221 Iwakata, S. & Grace, J.T. (1964) N.Y. J. Med. 64, 2279 Medium 199: Morgan, J.F. et al. (1950) Proc. Soc. Exp. Biol. Med. 73,1 Morgan, J.F. et al. (1955) J. Nat. Cancer Inst. 16, 557 a-MEM: Stanners, C.P. et al. (1971) Nature New Biology 230, 52 Stanners, C.P. & Goldberg, V.J. (1975) J. Gen. Virol. 29, 281 MEM: Eagle, H. (1955) Science 122, 501 Eagle, H. (1959) Science 130, 432 S-MEM: Eagle, H. (1959) Science 130, 432 Murashige & Skoog - Plant medium: Murashige, T. & Skoog, F. (1962) Physiol. Plant. 15, 473 NCTC 135 - Medium: Evans, V.J. et al. (1964) Exp. Cell Res. 36, 439 PBS - Powder: Dulbecco, R. & Vogt, M. (1957) J. Exp. Med. 106, 167 RPMI 1640 - Medium: Moore, G.E. et al. (1967) J.A.M.A. 199, 519 Schneider's Drosophila - Insect medium: Schneider, I. & Blumenthal, A. (1978) Biology and Genetics of Drosophila vol. 2A, Acad. Press, N.Y. pp. 266. TC-100 - Insect medium: Gardiner, G.R. & H. Stockdale (1975) J. Invert. Pathol. 25, 363 TNM-FH - Insect medium: Hink, W.F. (1970) Nature 266, 466 Hink, W.F. et al. (1974) In Vitro 9, 371 Waymouth's MB 752/1 - Medium: Waymouth, C. (1959) J. Nat. Cancer Inst. 22, 1003 William's Medium E: Williams, G.M. et al. (1971) Exp. Cell Res. 69, 106 Williams, G.M. & Gunn, J.M. (1974) Exp. Cell Res. 89, 139 Instructions for dissolving the powder to prepare liquid media Powdered media and salts are very hygroscopic and have to be stored under dry conditions. After opening one package you should dissolve the whole content at once. We recommend to produce 1X liquid medium from the powdered form, as the different amino acids may precipitate because of their low solubility. They are able to form salts which are of low solubility in concentrated solutions. If you have to add supplements to your medium, you can add them before filtration (unsterile) or after filtration (sterile). For dissolving the powdered medium please use bidistilled or deionized, pyrogen-free water. Procedure • The required quantity of powdered medium will be completely dissolved under stirring in approx. 90 % of the water quantity. The temperature of the water should be between +15 and +30˚C. The powder which remains in the package should be flushed out and dissolved with water. • If the powder is completely dissolved, add the sodium hydrogen carbonate and dissolve it completely as well. The required quantities are listed in the table on the following pages. • The pH (physiological optimum is pH 6.8 - 7.2) has to be adjusted with 1 N HCl or 1 N NaOH while stirring. The pH should be approx. 0.2 units lower than the target pH, because the pH will raise up a bit during filtration when the CO2 will leak out. • After adjusting the pH you have to add water up to the desired volume and mix it very well and filter immediately. • Store the medium at +4˚C protected from light. – 727 – General Catalog AppliChem

Transcript of Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage:...

Page 1: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Cell Culture Powder Media B

HS-No.: 38220000 Storage: 2-8˚CLGK: 10 - 13

S: 24/25hygroscopic

g

Comment:Composition

Powder media from AppliChem are mixed and processed according to the original formulations. To produce these media only raw materials of high quality are used. The single rawmaterial that is used in the media production is taken only from one lot, which is dried according to instructions. Shortly, after drying the raw materials, they will be grinded in specialmills to get a homogenous fine powder, that is packed immediately. Due to that the customer can easily dissolve our media. You will receive a weighing protocol on request.

HEPES-buffered media

HEPES-buffered media are often used, whenever the pH-value has to be stable in the media. With the addition of HEPES the pH value will get an additional stability in the pH range7.2 to 7.6 for pH-sensitive cell culture systems. Deviations of the pH value in the media are caused by the metabolism of the cultivated cells but also by changes of the CO2

concentration during incubation.

Storage

All powder media are produced without sodium hydrogen carbonate to increase the stability. Keeping them tightly closed is absolutely necessary as they are very hygroscopic. Forthis reason one package of powder media should always be dissolved completely. Storing dry and cold (+4 to +8˚C) will increase the shelf life, which is normally two years. Generallythe time for delivery will be one week.

Customer formulations

As a special service AppliChem offers the production of powder media according to the customer formulation for competitive prices - even in smaller quantities. In ourlaboratories we are able to consider most wishes regarding the composition of the powder media. The minimum order size is 20 liters with a delivery-date of two to threeweeks, generally. Please ask for our offer for your formulation giving us the exact composition and the quantity you need.

LiteratureBME: Eagle, H. (1965) Proc. Soc. Exp. Biol. Med. 89, 362DMEM: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396

Smith et al. (1960) Virology 12, 185DMEM/F12: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396

Smith et al. (1960) Virology 12, 185Barnes, D. & Sato, G. (1980) Anal. Biochem. 102, 255

Earle's buffered salt mixture: Earle, W. (1943) J. Nat. Cancer Inst.4, 165Gamborg's B5 - Plant salts: Gamborg O.L., Miller R.A., OjimaK. (1968) Exp. Cell Res. 50,151Glasgow - MEM (BHK 21): MacPherson, I. & Stoker, M. (1961) Virology 16, 147Grace's - Insect medium: Grace, T.D.C. (1962) Nature 195, 788

Grace, T.D.C. (1962) Nature216, 613Grace, T.D.C. (1966) Nature 211, 366

Ham's F10 - Medium: Ham, R.G. (1963) Exp. Cell Res. 29, 515Ham's F12K - Medium: Kaighn, M.E., (1973) Tissue Culture Methods and Applications , 54 (eds. Kruse & Patterson)Ham's F12 - Medium: Ham, R.G. (1965) Proc. Nat. Acad. Sci USA 53, 288Hanks' buffered salt mixture: Hanks', J.H. & Wallace, R.E. (1949) Proc. Soc. Exp. Biol. Med. 71, 196.Iscove's mod. Dulbecco's MEM (IMDM): Iscove, N., N. & Melchers, F (1978) J. Exp. Med. 147, 926

Iscove, N., N. et al. (1980) Exp. Cell Res. 126, 121IPL-41 - Medium: Weiss, S.A., G.C. Smith, S.S. Kalter & J.L. Vaughn. (1981) In Vitro 17, 495.Joklik - MEM: Eagle, H. (1959) Science 130, 432Leibovitz's L15 - Medium: Leibovitz, A. (1963) Amer. J. Hyg. 78, 173Linsmaier, Bednar & Skoog - Plant salts: Linsmaier E.M. & Skoog F. (1965) Physiol. Plantarum 18, 100McCoy's 5A - Medium (modified): McCoy, T.A. et al. (1959) Proc. Soc. Exp. Biol. Med. 100, 115

Hsu, T.C. & Kellogg, D.S. (1960) J. Nat. Cancer Inst. ,25, 221Iwakata, S. & Grace, J.T. (1964) N.Y. J. Med. 64, 2279

Medium 199: Morgan, J.F. et al. (1950) Proc. Soc. Exp. Biol. Med. 73, 1Morgan, J.F. et al. (1955) J. Nat. Cancer Inst. 16, 557

a-MEM: Stanners, C.P. et al. (1971) Nature New Biology 230, 52Stanners, C.P. & Goldberg, V.J. (1975) J. Gen. Virol. 29, 281

MEM: Eagle, H. (1955) Science 122, 501Eagle, H. (1959) Science 130, 432

S-MEM: Eagle, H. (1959) Science 130, 432Murashige & Skoog - Plant medium: Murashige, T. & Skoog, F. (1962) Physiol. Plant. 15, 473NCTC 135 - Medium: Evans, V.J. et al. (1964) Exp. Cell Res. 36, 439PBS - Powder: Dulbecco, R. & Vogt, M. (1957) J. Exp. Med. 106, 167RPMI 1640 - Medium: Moore, G.E. et al. (1967) J.A.M.A. 199, 519Schneider's Drosophila - Insect medium: Schneider, I. & Blumenthal, A. (1978) Biology and Genetics of Drosophila vol. 2A, Acad. Press, N.Y. pp. 266.TC-100 - Insect medium: Gardiner, G.R. & H. Stockdale (1975) J. Invert. Pathol. 25, 363TNM-FH - Insect medium: Hink, W.F. (1970) Nature 266, 466

Hink, W.F. et al. (1974) In Vitro 9, 371Waymouth's MB 752/1 - Medium: Waymouth, C. (1959) J. Nat. Cancer Inst. 22, 1003William's Medium E: Williams, G.M. et al. (1971) Exp. Cell Res. 69, 106

Williams, G.M. & Gunn, J.M. (1974) Exp. Cell Res. 89, 139

Instructions for dissolving the powder to prepare liquid mediaPowdered media and salts are very hygroscopic and have to be stored under dry conditions. After opening one package you should dissolve the whole content at once. Werecommend to produce 1X liquid medium from the powdered form, as the different amino acids may precipitate because of their low solubility. They are able to form salts which areof low solubility in concentrated solutions. If you have to add supplements to your medium, you can add them before filtration (unsterile) or after filtration (sterile).For dissolving the powdered medium please use bidistilled or deionized, pyrogen-free water.Procedure• The required quantity of powdered medium will be completely dissolved under stirring in approx. 90 % of the water quantity. The temperature of the water should be between +15and +30˚C. The powder which remains in the package should be flushed out and dissolved with water.• If the powder is completely dissolved, add the sodium hydrogen carbonate and dissolve it completely as well. The required quantities are listed in the table on the following pages.• The pH (physiological optimum is pH 6.8 - 7.2) has to be adjusted with 1 N HCl or 1 N NaOH while stirring. The pH should be approx. 0.2 units lower than the target pH, because thepH will raise up a bit during filtration when the CO2 will leak out.• After adjusting the pH you have to add water up to the desired volume and mix it very well and filter immediately.• Store the medium at +4˚C protected from light.

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Page 2: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

BME, Basal medium with Earle's salts

BME, Basal medium with Earle's salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1310,1000 1 LA1310,5000 5 LA1310,9010 10 LA1310,9050 50 L

BME, Basal medium with Earle's salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1311,1000 1 LA1311,5000 5 LA1311,9010 10 LA1311,9050 50 L

10X BME, Basal medium with Earle's salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1977,1000 1 LA1977,5000 5 LA1977,9010 10 LA1977,9050 50 L

10X BME, Basal medium with Earle's salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1983,1000 1 LA1983,5000 5 LA1983,9010 10 LA1983,9050 50 L

BME, Basal medium with Earle's salts (diploide) Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1984,1000 1 LA1984,5000 5 LA1984,9010 10 LA1984,9050 50 L

BME, Basal medium with Earle's salts (diploide) Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1312,1000 1 LA1312,5000 5 LA1312,9010 10 LA1312,9050 50 L

BME, Basal medium with Earle's salts, autoclavable Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1976,1000 1 LA1976,5000 5 LA1976,9010 10 LA1976,9050 50 L

BME, Basal medium with Earle's salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1915,1000 1 LA1915,5000 5 LA1915,9010 10 LA1915,9050 50 L

BME, Basal medium with Earle's salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1916,1000 1 LA1916,5000 5 LA1916,9010 10 LA1916,9050 50 L

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Page 3: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

BME, Basal medium with Hanks' salts

BME, Basal medium with Hanks' salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1917,1000 1 LA1917,5000 5 LA1917,9010 10 LA1917,9050 50 L

BME, Basal medium with Hanks' salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1313,1000 1 LA1313,5000 5 LA1313,9010 10 LA1313,9050 50 L

10X BME, Basal medium with Hanks' salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1990,1000 1 LA1990,5000 5 LA1990,9010 10 LA1990,9050 50 L

10X BME, Basal medium with Hanks' salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1988,1000 1 LA1988,5000 5 LA1988,9010 10 LA1988,9050 50 L

BME, Basal medium with Hanks' salts, autoclavable Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1978,1000 1 LA1978,5000 5 LA1978,9010 10 LA1978,9050 50 L

BME, Basal medium with Hanks' salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1918,1000 1 LA1918,5000 5 LA1918,9010 10 LA1918,9050 50 L

BME, Basal medium with Hanks' salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1919,1000 1 LA1919,5000 5 LA1919,9010 10 LA1919,9050 50 L

DMEM (Dulbecco's mod. Eagle - Media)

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewithout L-Glutaminewith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1924,1000 1 LA1924,5000 5 LA1924,9010 10 LA1924,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewith L-Glutaminewith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1314,1000 1 LA1314,5000 5 LA1314,9010 10 LA1314,9050 50 L

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Page 4: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewithout L-Glutaminewith Sodium pyruvatewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA0826,1000 1 LA0826,5000 5 LA0826,9010 10 LA0826,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewith L-Glutaminewith Sodium pyruvatewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3134,1000 1 LA3134,5000 5 LA3134,9010 10 LA3134,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewithout L-Glutaminewith 25 mM HEPESwith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1925,1000 1 LA1925,5000 5 LA1925,9010 10 LA1925,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 1.0 g/L D(+)-Glucosewith L-Glutaminewith 25 mM HEPESwith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1315,1000 1 LA1315,5000 5 LA1315,9010 10 LA1315,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewithout Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1926,1000 1 LA1926,5000 5 LA1926,9010 10 LA1926,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewith L-Glutaminewithout Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1316,1000 1 LA1316,5000 5 LA1316,9010 10 LA1316,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1985,1000 1 LA1985,5000 5 LA1985,9010 10 LA1985,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewith L-Glutaminewith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1986,1000 1 LA1986,5000 5 LA1986,9010 10 LA1986,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewith Sodium pyruvatewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3137,1000 1 LA3137,5000 5 LA3137,9010 10 LA3137,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewith L-Glutaminewith Sodium pyruvatewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3139,1000 1 LA3139,5000 5 LA3139,9010 10 LA3139,9050 50 L

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Page 5: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewith 25 mM HEPESwithout Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1950,1000 1 LA1950,5000 5 LA1950,9010 10 LA1950,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewith 25 mM HEPESwithout Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1965,1000 1 LA1965,5000 5 LA1965,9010 10 LA1965,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewithout L-Glutaminewith 25 mM HEPESwith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1979,1000 1 LA1979,5000 5 LA1979,9010 10 LA1979,9050 50 L

DMEM (Dulbecco's mod. Eagle - Medium) Bwith 4.5 g/L D(+)-Glucosewith L-Glutaminewith 25 mM HEPESwith Sodium pyruvatewithout Sodium hydrogen carbonate

Order-No. QuantityA1951,1000 1 LA1951,5000 5 LA1951,9010 10 LA1951,9050 50 L

DMEM/F12 (1:1) - Media

DMEM/F12 (1:1) - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1920,1000 1 LA1920,5000 5 LA1920,9010 10 LA1920,9050 50 L

DMEM/F12 (1:1) - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1318,1000 1 LA1318,5000 5 LA1318,9010 10 LA1318,9050 50 L

DMEM/F12 (1:1) - Medium Bwithout L-Glutaminewith 15 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1922,1000 1 LA1922,5000 5 LA1922,9010 10 LA1922,9050 50 L

DMEM/F12 (1:1) - Medium Bwith L-Glutaminewith 15 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1317,1000 1 LA1317,5000 5 LA1317,9010 10 LA1317,9050 50 L

DMEM/F12 (1:1) - Medium Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1923,1000 1 LA1923,5000 5 LA1923,9010 10 LA1923,9050 50 L

DMEM/F12 (1:1) - Medium Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1921,1000 1 LA1921,5000 5 LA1921,9010N 10 LA1921,9050 50 L

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Page 6: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Earle's balanced salts (EBSS)

Earle's balanced salts (EBSS) Bwith 1.0 g/L D(+)-Glucosewith Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA1712,5000 5 LA1712,9010 10 LA1712,9050 50 L

Earle's balanced salts (EBSS) Bwith 1.0 g/L D(+)-Glucosewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA2039,5000 5 LA2039,9010 10 LA2039,9050 50 L

Earle's balanced salts (EBSS) Bwith 1.0 g/L D(+)-Glucosewith Phenol redwithout Calcium and Magnesiumwithout Sodium hydrogen carbonate

Order-No. QuantityA3026,5000 5 LA3026,9010 10 LA3026,9050 50 L

Earle's balanced salts (EBSS) Bwith 1.0 g/L D(+)-Glucosewithout Calcium and Magnesiumwithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3127,5000 5 LA3127,9010 10 LA3127,9050 50 L

Gamborg's B5 Plant salts B

Order-No. QuantityA1911,5000 5 LA1911,9010 10 LA1911,9050 50 L

Glasgow - MEM (BHK 21)

Glasgow - MEM (BHK 21) Bwithout L-Glutaminewithout Tryptose/Phosphate-Bouillonwithout Sodium hydrogen carbonate

Order-No. QuantityA2024,1000 1 LA2024,5000 5 LA2024,9010 10 LA2024,9050 50 L

Glasgow - MEM (BHK 21) Bwith L-Glutaminewithout Tryptose/Phosphate-Bouillonwithout Sodium hydrogen carbonate

Order-No. QuantityA1320,1000 1 LA1320,5000 5 LA1320,9010 10 LA1320,9050 50 L

Glasgow - MEM (BHK 21) Bwithout L-Glutaminewith Tryptose/Phosphate-Bouillonwithout Sodium hydrogen carbonate

Order-No. QuantityA2025,1000 1 LA2025,5000 5 LA2025,9010 10 LA2025,9050 50 l

Glasgow - MEM (BHK 21) Bwith L-Glutaminewith Tryptose/Phosphate-Bouillonwithout Sodium hydrogen carbonate

Order-No. QuantityA1321,1000 1 LA1321,5000 5 LA1321,9010 10 LA1321,9050 50 L

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Grace's - Insect medium

Grace's - Insect medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2023,1000 1 LA2023,5000 5 LA2023,9010 10 LA2023,9050 50 L

Grace's - Insect medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1529,1000 1 LA1529,5000 5 LA1529,9010 10 LA1529,9050 50 L

Ham's F10 - Medium

Ham's F10 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1970,1000 1 LA1970,5000 5 LA1970,9010 10 LA1970,9050 50 L

Ham's F10 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1525,1000 1 LA1525,5000 5 LA1525,9010 10 LA1525,9050 50 L

Ham's F10 - Medium Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1989,1000 1 LA1989,5000 5 LA1989,9010 10 LA1989,9050 50 L

Ham's F10 - Medium Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1982,1000 1 LA1982,5000 5 LA1982,9010 10 LA1982,9050 50 L

Ham's F10 - Medium Bwithout L-Glutaminewith 25 mM HEPESwith 1.2 g/L Sodium hydrogen carbonate

Order-No. QuantityA2026,1000 1 LA2026,5000 5 LA2026,9010 10 LA2026,9050 50 L

Ham's F10 - Medium Bwith L-Glutaminewith 25 mM HEPESwith 1.2 g/L Sodium hydrogen carbonate

Order-No. QuantityA1952,1000N 1 LA1952,5000 5 LA1952,9010 10 LA1952,9050 50 L

Ham's F12 - Medium

Ham's F12 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1948,1000 1 LA1948,5000 5 LA1948,9010 10 LA1948,9050 50 L

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Ham's F12 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1526,1000 1 LA1526,5000 5 LA1526,9010 10 LA1526,9050 50 L

Ham's F12 - Medium Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1969,1000 1 LA1969,5000 5 LA1969,9010 10 LA1969,9050 50 L

Ham's F12 - Medium Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1953,1000 1 LA1953,5000 5 LA1953,9010 10 LA1953,9050 50 L

Ham's F12K - Medium

Ham's F12K - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1963,1000 1 LA1963,5000 5 LA1963,9010 10 LA1963,9050 50 L

Ham's F12K - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1927,1000 1 LA1927,5000 5 LA1927,9010 10 LA1927,9050 50 L

Hanks' balanced salts (HBSS)

Hanks' balanced salts (HBSS) Bwith 1.0 g/L D(+)-Glucosewith Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA1711,5000 5 LA1711,9010 10 LA1711,9050 50 L

Hanks' balanced salts (HBSS) Bwith 1.0 g/L D(+)-Glucosewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3140,5000 5 LA3140,9010 10 LA3140,9050 50 L

Hanks' balanced salts (HBSS) Bwith 1.0 g/L D(+)-Glucosewithout Calcium and Magnesiumwith Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA2729,5000 5 LA2729,9010 10 LA2729,9050 50 L

Hanks' balanced salts (HBSS) Bwith 1.0 g/L D(+)-Glucosewithout Calcium and Magnesiumwithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA2512,5000 5 LA2512,9010 10 LA2512,9050 50 L

HEPES - balanced salts B

Order-No. QuantityA0629,1000 1 LA0629,5000 5 LA0629,9010 10 L

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IPL 41 - Medium

IPL 41 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2027,1000 1 LA2027,5000 5 LA2027,9010 10 LA2027,9050 50 L

IPL 41 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1964,1000 1 LA1964,5000 5 LA1964,9010 10 LA1964,9050 50 L

Iscove's mod. Dulbecco's - Medium (IMDM)

Iscove's mod. Dulbecco's - Medium (IMDM) Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2028,1000 1 LA2028,5000 5 LA2028,9010 10 LA2028,9050 50 L

Iscove's mod. Dulbecco's - Medium (IMDM) Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1909,1000 1 LA1909,5000 5 LA1909,9010 10 LA1909,9050 50 L

Iscove's mod. Dulbecco's - Medium (IMDM) Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA2029,1000 1 LA2029,5000 5 LA2029,9010 10 LA2029,9050 50 L

Iscove's mod. Dulbecco's - Medium (IMDM) Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1322,1000 1 LA1322,5000 5 LA1322,9010 10 LA1322,9050 50 L

Joklik - MEM, modified for spinner culture

Joklik - MEM, modified for spinner culture Bwith Earle's salt (modified)without L-Glutaminewithout Antibioticswithout Calcium chloridewithout Sodium hydrogen carbonate

Order-No. QuantityA1974,1000 1 LA1974,5000 5 LA1974,9010 10 LA1974,9050 50 L

Joklik - MEM, modified for spinner culture Bwith Earle's salts (modified)with L-Glutaminewithout Antibioticswithout Calcium chloridewithout Sodium hydrogen carbonate

Order-No. QuantityA1539,1000 1 LA1539,5000 5 LA1539,9010 10 LA1539,9050 50 L

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Leibovitz's L15 - Medium

Leibovitz's L15 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1967,1000 1 LA1967,5000 5 LA1967,9010 10 LA1967,9050 50 L

Leibovitz's L15 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1323,1000 1 LA1323,5000 5 LA1323,9010 10 LA1323,9050 50 L

Leibovitz's L15 - Medium Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1968,1000 1 LA1968,5000 5 LA1968,9010 10 LA1968,9050 50 L

Leibovitz's L15 - Medium Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1954,1000 1 LA1954,5000 5 LA1954,9010 10 LA1954,9050 50 L

Linsmaier, Bednar & Skoog Plant salts B

Order-No. QuantityA0591,5000 5 LA0591,9010 10 LA0591,9050 50 L

McCoy's 5A - Medium (modified)

McCoy's 5A - Medium (modified) Bwithout L-Glutaminewithout Serumwithout Sodium hydrogen carbonate

Order-No. QuantityA2034,1000 1 LA2034,5000 5 LA2034,9010 10 LA2034,9050 50 L

McCoy's 5A - Medium (modified) Bwith L-Glutaminewithout Serumwithout Sodium hydrogen carbonate

Order-No. QuantityA1324,1000 1 LA1324,5000 5 LA1324,9010 10 LA1324,9050 50 L

McCoy's 5A - Medium (modified) Bwithout L-Glutaminewith 25 mM HEPESwithout Serumwithout Sodium hydrogen carbonate

Order-No. QuantityA2035,1000 1 LA2035,5000 5 LA2035,9010 10 LA2035,9050 50 L

McCoy's 5A - Medium (modified) Bwith L-Glutaminewith 25 mM HEPESwithout Serumwithout Sodium hydrogen carbonate

Order-No. QuantityA1955,1000 1 LA1955,5000 5 LA1955,9010 10 LA1955,9050 50 L

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Medium 199 with Earle's Salts

Medium 199 with Earle's Salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2022,1000 1 LA2022,5000N 5 LA2022,9010 10 LA2022,9050 50 L

Medium 199 with Earle's Salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1325,1000 1 LA1325,5000 5 LA1325,9010 10 LA1325,9050 50 L

Medium 199 with Earle's Salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA2030,1000 1 LA2030,5000 5 LA2030,9010 10 LA2030,9050 50 L

Medium 199 with Earle's Salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1326,1000 1 LA1326,5000 5 LA1326,9010 10 LA1326,9050 50 L

Medium 199 with Hanks' Salts

Medium 199 with Hanks' Salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2031,1000 1 LA2031,5000 5 LA2031,9010 10 LA2031,9050 50 L

Medium 199 with Hanks' Salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1327,1000 1 LA1327,5000 5 LA1327,9010 10 LA1327,9050 50 L

Medium 199 with Hanks' Salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA2021,1000 1 LA2021,5000 5 LA2021,9010 10 LA2021,9050 50 L

Medium 199 with Hanks' Salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1328,1000 1 LA1328,5000 5 LA1328,9010 10 LA1328,9050 50 L

aa-MEM

aa-MEM Bwithout L-Glutaminewithout Deoxyribonucleosideswithout Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1971,1000 1 LA1971,5000 5 LA1971,9010 10 LA1971,9050 50 L

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aa-MEM Bwith L-Glutaminewithout Deoxyribonucleosideswithout Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1330,1000 1 LA1330,5000 5 LA1330,9010 10 LA1330,9050 50 L

aa-MEM Bwithout L-Glutaminewith Ribonucleosideswith Deoxyribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1913,1000 1 LA1913,5000 5 LA1913,9010 10 LA1913,9050 50 L

aa-MEM Bwith L-Glutaminewith Deoxyribonucleosideswith Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1329,1000 1 LA1329,5000 5 LA1329,9010 10 LA1329,9050 50 L

aa-MEM Bwithout L-Glutaminewith 25 mM HEPESwithout Deoxyribonucleosideswithout Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1980,1000 1 LA1980,5000 5 LA1980,9010 10 LA1980,9050 50 L

aa-MEM Bwith L-Glutaminewith 25 mM HEPESwithout Deoxyribonucleosideswithout Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1972,1000 1 LA1972,5000 5 LA1972,9010 10 LA1972,9050 50 L

aa-MEM Bwithout L-Glutaminewith 25 mM HEPESwith Deoxyribonucleosideswith Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1949,1000 1 LA1949,5000 5 LA1949,9010 10 LA1949,9050 50 L

aa-MEM Bwith L-Glutaminewith 25 mM HEPESwith Desoxyribonucleosideswith Ribonucleosideswithout Sodium hydrogen carbonate

Order-No. QuantityA1914,1000 1 LA1914,5000 5 LA1914,9010 10 LA1914,9050 50 L

MEM with Earle's salts

MEM with Earle's salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1331,1000 1 LA1331,5000 5 LA1331,9010 10 LA1331,9050 50 L

MEM with Earle's salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1332,1000 1 LA1332,5000 5 LA1332,9010 10 LA1332,9050 50 L

MEM with Earle's salts, autoclavable Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2597,1000 1 LA2597,5000 5 LA2597,9010 10 LA2597,9050 50 L

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MEM with Earle's salts Bwithout L-Glutaminewith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA2018,1000N 1 LA2018,5000 5 LA2018,9010N 10 LA2018,9050 50 L

MEM with Earle's salts Bwith L-Glutaminewith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA1334,1000 1 LA1334,5000 5 LA1334,9010 10 LA1334,9050 50 L

MEM with Earle's salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA2019,1000 1 LA2019,5000 5 LA2019,9010 10 LA2019,9050 50 L

MEM with Earle's salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1333,1000 1 LA1333,5000 5 LA1333,9010 10 LA1333,9050 50 L

MEM with Earle's salts Bwithout L-Glutaminewith 25 mM HEPESwith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA2032,1000 1 LA2032,5000 5 LA2032,9010 10 LA2032,9050 50 L

MEM with Earle's salts Bwith L-Glutaminewith 25 mM HEPESwith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA1956,1000 1 LA1956,5000 5 LA1956,9010 10 LA1956,9050 50 L

MEM with Hanks' salts

MEM with Hanks' salts Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1957,1000 1 LA1957,5000 5 LA1957,9010 10 LA1957,9050 50 L

MEM with Hanks' salts Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1335,1000 1 LA1335,5000 5 LA1335,9010 10 LA1335,9050 50 L

MEM with Hanks' salts Bwithout L-Glutaminewith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA2041,1000 1 LA2041,5000 5 LA2041,9010 10 LA2041,9050 50 L

MEM with Hanks' salts Bwith L-Glutaminewith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA1337,1000 1 LA1337,5000 5 LA1337,9010 10 LA1337,9050 50 L

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MEM with Hanks'salts Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA2042,1000 1 LA2042,5000 5 LA2042,9010 10 LA2042,9050 50 L

MEM with Hanks' salts Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1336,1000 1 LA1336,5000 5 LA1336,9010 10 LA1336,9050 50 L

MEM with Hanks' salts Bwithout L-Glutaminewith 25 mM HEPESwith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA2043,1000 1 LA2043,5000 5 LA2043,9010 10 LA2043,9050 50 L

MEM with Hanks' salts Bwith L-Glutaminewith 25 mM HEPESwith NEAAwithout Sodium hydrogen carbonate

Order-No. QuantityA1958,1000 1 LA1958,5000 5 LA1958,9010 10 LA1958,9050 50 L

S-MEM, modified for spinner culture

S-MEM, modified for spinner culture Bwith Earle's salt (modified)without L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1338,1000 1 LA1338,5000 5 LA1338,9010 10 LA1338,9050 50 L

S-MEM, modified for spinner culture Bwith Earle's salts (modified)with L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1308,1000 1 LA1308,5000 5 LA1308,9010 10 LA1308,9050 50 L

S-MEM, modified for spinner culture, autoclavable Bwith Earle's salt (modified)without L-Glutaminewithout Sodium hydrogen carbonateautoclavable

Order-No. QuantityA1975,1000 1 LA1975,5000 5 LA1975,9010 10 LA1975,9050 50 L

NCTC 135 - Medium

NCTC 135 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2020,1000 1 LA2020,5000 5 LA2020,9010 10 LA2020,9050 50 L

NCTC 135 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1528,1000 1 LA1528,5000 5 LA1528,9010 10 LA1528,9050 50 L

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PBS buffer (Dulbecco's) - PowderPhosphate buffered saline, Phosphatebuffered saline, 10X concentrated

PBS buffer (1X, Dulbecco's) - Powder BOrder-No. QuantityA0964,9010 10 LA0964,9050 50 LA0964,9100 100 L

PBS buffer (10X Dulbecco's) - Powder BOrder-No. QuantityA0965,9010 10 LA0965,9050 50 LA0965,9100 100 L

PBS buffer (1X Dulbecco's) - Powder Bwith 0,01 % Thimerosalwith 0,02 % EDTA

Order-No. QuantityA1908,9010 10 LA1908,9050 50 LA1908,9100 100 L

RPMI 1640 - Medium

RPMI 1640 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1959,1000 1 LA1959,5000 5 LA1959,9010 10 LA1959,9050 50 L

RPMI 1640 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1339,1000 1 LA1339,5000 5 LA1339,9010 10 LA1339,9050 50 L

RPMI 1640 - Medium Bwithout L-Glutaminewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA2040,1000 1 LA2040,5000 5 LA2040,9010 10 LA2040,9050 50 L

RPMI 1640 - Medium Bwith L-Glutaminewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3135,1000 1 LA3135,5000 5 LA3135,9010 10 LA3135,9050 50 L

RPMI 1640 - Medium Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1960,1000 1 LA1960,5000 5 LA1960,9010 10 LA1960,9050 50 L

RPMI 1640 - Medium Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1538,1000 1 LA1538,5000 5 LA1538,9010 10 LA1538,9050 50 L

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Click - RPMI - Medium

Click - RPMI - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2044,1000 1 LA2044,5000 5 LA2044,9010 10 LA2044,9050 50 L

Click - RPMI - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2504,1000 1 LA2504,5000 5 LA2504,9010 10 LA2504,9050 50 L

Schneider's Drosophila - Insect medium

Schneider's Drosophila - Insect medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2036,1000N 1 LA2036,5000 5 LA2036,9010N 10 LA2036,9050 50 L

Schneider's Drosophila - Insect medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1533,1000 1 LA1533,5000 5 LA1533,9010 10 LA1533,9050 50 L

TC 100 - Insect medium

TC 100 - Insect medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2033,1000 1 LA2033,5000 5 LA2033,9010 10 LA2033,9050 50 L

TC 100 - Insect medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA2017,1000 1 LA2017,5000 5 LA2017,9010 10 LA2017,9050 50 L

TNM-FH - Insect medium (modifiziertes Grace's Insektenmedium)modified Grace's Insect medium

TNM-FH - Insect medium (modified Grace's Insect medium) Bwithout L-Glutaminewith Lactalbumin hydrolysatewith Yeast extractswithout Sodium hydrogen carbonate

Order-No. QuantityA2037,1000 1 LA2037,5000 5 LA2037,9010 10 LA2037,9050 50 L

TNM-FH - Insect medium (modified Grace's Insect medium) Bwith L-Glutaminewith Lactalbumin hydrolysatewith Yeast extractswithout Sodium hydrogen carbonate

Order-No. QuantityA1570,1000 1 LA1570,5000 5 LA1570,9010 10 LA1570,9050 50 L

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Waymouth's MB 752/1 - Medium

Waymouth's MB 752/1 - Medium Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1973,1000 1 LA1973,5000 5 LA1973,9010N 10 LA1973,9050 50 L

Waymouth's MB 752/1 - Medium Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1527,1000 1 LA1527,5000 5 LA1527,9010 10 LA1527,9050 50 L

Waymouth's MB 752/1 - Medium Bwithout L-Glutaminewith 20 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1981,1000 1 LA1981,5000 5 LA1981,9010 10 LA1981,9050 50 L

Waymouth's MB 752/1 - Medium Bwith L-Glutaminewith 20 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1961,1000 1 LA1961,5000 5 LA1961,9010 10 LA1961,9050 50 L

William's Media E

William's Medium E Bwithout L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1309,1000 1 LA1309,5000 5 LA1309,9010 10 LA1309,9050 50 L

William's Medium E Bwith L-Glutaminewithout Sodium hydrogen carbonate

Order-No. QuantityA1910,1000 1 LA1910,5000 5 LA1910,9010 10 LA1910,9050 50 L

William's Medium E Bwithout L-Glutaminewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3029,1000 1 LA3029,5000 5 LA3029,9010 10 LA3029,9050 50 L

William's Medium E Bwith L-Glutaminewithout Phenol redwithout Sodium hydrogen carbonate

Order-No. QuantityA3136,1000 1 LA3136,5000 5 LA3136,9010 10 LA3136,9050 50 L

William's Medium E Bwithout L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1966,1000N 1 LA1966,5000 5 LA1966,9010 10 LA1966,9050 50 L

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William's Medium E Bwith L-Glutaminewith 25 mM HEPESwithout Sodium hydrogen carbonate

Order-No. QuantityA1962,1000 1 LA1962,5000 5 LA1962,9010 10 LA1962,9050 50 L

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BME, Basal medium with Earle's salts A1310 2.200

BME, Basal medium with Earle's salts A1311 2.200

10X BME, Basal medium with Earle's salts A1977 2.200 / L 1X

10X BME, Basal medium with Earle's salts A1983 2.200 / L 1X

BME, Basal medium with Earle's salts, with HEPES A1915 2.200

BME, Basal medium with Earle's salts, with HEPES A1916 2.200

BME, Basal medium with Earle's (diploide) A1984 2.200

BME, Basal medium with Earle's (diploide) A1312 2.200

BME, Basal medium with Hanks' salts A1917 0.350

BME, Basal medium with Hanks' salts A1313 0.350

10X BME, Basal medium with Hanks' salts A1990 0.350 / L 1X

10X BME, Basal medium with Hanks' salts A1988 0.350 / L 1X

BME, Basal medium with Hanks' salts; autoclavable A1976 30 ml 7.5 % - Solution / L after autoclaving

BME, Basal medium with Hanks' salts; autoclavable A1978 5 ml 7.5 % - Solution / L after autoclaving

BME, Basal medium with Hanks' salts, with HEPES A1918 0.350

BME, Basal medium with Hanks' salts, with HEPES A1919 0.350

DMEM - Medium A1924 3.700

DMEM - Medium A1314 3.700

DMEM - Medium with HEPES A1925 0.850

DMEM - Medium with HEPES A1315 0.850

DMEM - Medium A1926 3.700

DMEM - Medium A1316 3.700

DMEM - Medium A1985 3.700

DMEM - Medium A1986 3.700

DMEM - Medium with HEPES A1950 0.850

DMEM - Medium with HEPES A1965 0.850

DMEM - Medium with HEPES A1979 0.850

DMEM - Medium with HEPES A1951 0.850

DMEM - Medium A0826 3.700

DMEM - Medium A3134 3.700

DMEM - Medium A3137 3.700

DMEM - Medium A3139 3.700

DMEM/F12 (1:1) - Medium A1920 1.200

DMEM/F12 (1:1) - Medium A1318 1.200

DMEM/F12 (1:1) - Medium with 15 mM HEPES A1922 1.200

DMEM/F12 (1:1) - Medium with 15 mM HEPES A1317 1.200

DMEM/F12 (1:1) - Medium with 25 mM HEPES A1923 1.200

DMEM/F12 (1:1) - Medium with 25 mM HEPES A1921 1.200

Earle's balanced salts A1712 2.200

Earle's balanced salts A2039 2.200

Earle's balanced salts A3026 2.200

Earle's balanced salts A3127 2.200

Gamborg's B5 - Plant salts A1911 none

Glasgow - MEM (BHK 21) A2024 2.750

Glasgow - MEM (BHK 21) A1320 2.750

Glasgow - MEM (BHK 21) A2025 2.750

Glasgow - MEM (BHK 21) A1321 2.750

Grace's - Insect medium A2023 0.350

Grace's - Insect medium A1529 0.350

Ham's F10 - Medium A1970 1.200

Ham's F10 - Medium A1525 1.200

Ham's F10 - Medium with HEPES A1989 1.200

Ham's F10 - Medium with HEPES A1982 1.200

Ham's F10 - Medium with HEPES A1952 1.200

Ham's F10 - Medium with HEPES A2026 1.200

Ham's F12K - Medium A1963 2.500

Ham's F12K - Medium A1927 2.500

Ham's F12 - Medium A1948 1.176

Ham's F12 - Medium A1526 1.176

Ham's F12 - Medium with HEPES A1969 1.176

Ham's F12 - Medium with HEPES A1953 1.176

Ham's F12K - Medium A1963 2.500

Ham's F12K - Medium A1927 2.500

Hanks' balanced salts A1711 0.350

Hanks' balanced salts A3140 0.350

Description Article-No. NaHCO3 (g / L)

Table for the addition of sodium hydrogen carbonate

– 745 –

#250448 APPLICHEM - GENERAL KATALOG 2008 INHALT [745 <745> 748] 26.2.08

General CatalogAppliChem

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Page 20: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Hanks' balanced salts A2729 0.350

Hanks' balanced salts A2512 0.350

HEPES balanced salts A0629 none

Iscove's mod. Dulbecco's - Medium (IMDM) A2028 3.700

Iscove's mod. Dulbecco's - Medium (IMDM) A1909 3.700

Iscove's mod. Dulbecco's - Medium (IMDM) A2029 3.024

Iscove's mod. Dulbecco's - Medium (IMDM) A1322 3.024

IPL 41 - Medium A2027 0.350

IPL 41 - Medium A1964 0.350

Joklik - MEM, modified for spinner culture A1974 2.000

Joklik - MEM, modified for spinner culture A1539 2.000

Leibovitz's L-15 - Medium A1967 none

Leibovitz's L-15 - Medium A1323 none

Leibovitz's L-15 - Medium with HEPES A1968 none

Leibovitz's L-15 - Medium with HEPES A1954 none

Linsmaier, Bednar & Skoog Plant salts A0591 none

McCoy's 5A - Medium (modified) A2034 2.200

McCoy's 5A - Medium (modified) A1324 2.200

McCoy's 5A - Medium (modified) A2035 2.200

McCoy's 5A - Medium (modified) A1955 2.200

Medium 199 with Earle's salts A2022 2.200

Medium 199 with Earle's salts A1325 2.200

Medium 199 with Earle's salts, with HEPES A2030 0.850

Medium 199 with Earle's salts, with HEPES A1326 0.850

Medium 199 with Hanks' salts A2031 0.350

Medium 199 with Hanks' salts A1327 0.350

Medium 199 with Hanks' salts, with HEPES A2021 0.350

Medium 199 with Hanks' salts, with HEPES A1328 0.350

a - MEM A1971 2.200

a - MEM A1330 2.200

a - MEM A1913 2.200

a - MEM A1329 2.200

a - MEM with HEPES A1980 0.850

a - MEM with HEPES A1972 0.850

a - MEM with HEPES A1949 0.850

a - MEM with HEPES A1914 0.850

MEM with Earle's salts A1331 2.200

MEM with Earle's salts A1332 2.200

MEM with Earle's salts A2018 2.200

MEM with Earle's salts A1334 2.200

MEM with Earle's salts, with HEPES A2019 0.850

MEM with Earle's salts, with HEPES A1333 0.850

MEM with Earle's salts, with HEPES A2032 0.850

MEM with Earle's salts, with HEPES A1956 0.850

MEM with Earle's salts; autoclavable A2597 30 ml 7.5 % - Solution / L after autoclaving

MEM with Hanks' salts A1957 0.350

MEM with Hanks' salts A1335 0.350

MEM with Hanks' salts A2041 0.350

MEM with Hanks' salts A1337 0.350

MEM with Hanks' salts, with HEPES A2042 0.350

MEM with Hanks' salts, with HEPES A1336 0.350

MEM with Hanks' salts, with HEPES A2043 0.350

MEM with Hanks' salts, with HEPES A1958 0.350

S-MEM, modified for spinner culture A1338 2.200

S-MEM, modified for spinner culture A1308 2.200

S-MEM, modified for spinner culture; autoclavable A1975 30 ml 7.5 % - Solution / L after autoclaving

Murashige & Skoog - Plant salts A1534 none

Murashige & Skoog - Plant salts A1535 none

NCTC 135 - Medium A2020 2.200

NCTC 135 - Medium A1528 2.200

PBS buffer (1X, Dulbecco) - Powder A0964 none

PBS buffer (10X, Dulbecco) - Powder A0965 none

PBS buffer (1X, Dulbecco) - Powder A1908 none

RPMI 1640 A1959 2.200

RPMI 1640 A1339 2.200

Description Article-No. NaHCO3 (g / L)

Table for the addition of sodium hydrogen carbonate

#250448 APPLICHEM - GENERAL KATALOG 2008 INHALT [745 <746> 748] 26.2.08

– 746 –

AppliChem General Catalog

Page 21: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

RPMI 1640 with HEPES A1960 0.850

RPMI 1640 with HEPES A1538 0.850

RPMI 1640 A2040 2.000

RPMI 1640 A3135 2.000

Click-RPMI A2044 1.175

Click-RPMI A2504 1.175

Schneider's Drosophila - Insect medium A2036 0.400

Schneider's Drosophila - Insect medium A1533 0.400

TC-100 - Medium A2033 0.350

TC-100 - Medium A2017 0.350

TNM-FH - Insect medium A2037 0.350

TNM-FH - Insect medium A1570 0.350

Tris - Citrate balanced salts A0692 none

Waymouth's MB 752/1 - Medium A1973 2.240

Waymouth's MB 752/1 - Medium A1527 2.240

Waymouth's MB 752/1 - Medium with 20 mM HEPES A1981 0.850

Waymouth's MB 752/1 - Medium with 20 mM HEPES A1961 0.850

William's Medium E A1309 2.200

William's Medium E A1910 2.200

William's Medium E with HEPES A1966 0.850

William's Medium E with HEPES A1962 0.850

William's Medium E A3029 2.200

William's Medium E A3136 2.200

Description Article-No. NaHCO3 (g / L)

Table for the addition of sodium hydrogen carbonate

– 747 –

#250448 APPLICHEM - GENERAL KATALOG 2008 INHALT [745 <747> 748] 26.2.08

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Page 22: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

BME, Basal medium with Earle's saltsComponents A1310 A1311 A1977 A1983 A1915 A1916 A1984 A1312 A1976

autoclava-ble

Inorganic salts Calcium chloridex 2H2O

264.92 264.92 2649.20 2649.20 264.92 264.92 264.92 264.92 264.92

Magnesiumchlorideanhydrous

93.68 93.68

Magnesiumsulfate dried

139,52 139,52 1395,20 1395,20 139,52 139,52 139,52

Potassiumchloride

400.00 400.00 4000.00 4000.00 400.00 400.00 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 68000.00 68000.00 6300.00 6300.00 6800.00 6800.00 6800.00

Sodiumdihydrogenphosphate x H2O

140.00 140.00 1400.00 1400.00 140.00 140.00 140.00 140.00 140.00

OtherComponents

D(+)-Glucoseanhydrous

1000.00 1000.00 10000.00 10000.00 1000.00 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00

Phenol red 10.00 10.00 100.00 100.00 10.00 10.00 10.00 10.00 6.00

Succinic acid 75.00

Succinic acidsodium salt

100.00

Amino acids L-Arginine x HCl 21.00 21.00 210.00 210.00 21.00 21.00 21.00 21.00 21.00

L-Cystine 12.00 12.00 120.00 120.00 12.00 12.00 12.00 12.00 12.00

L-Glutamine 0 292.00 0 2920.00 0 292.00 0 292.00 0

L-Histidine Base 8.00 8.00 80.00 80.00 8.00 8.00 8.00 8.00

L-Histidine x HClx H2O

10.81 10.81 15.00

L-Isoleucine 26.00 26.00 260.00 260.00 26.00 26.00 26.00 26.00 26.00

L-Leucine 26.00 26.00 260.00 260.00 26.00 26.00 26.00 26.00 26.00

L-Lysine x HCl 36.47 36.47 364.70 364.70 36.47 36.47 36.47 36.47 36.47

L-Methionine 7.50 7.50 75.00 75.00 7.50 7.50 7.50 7.50 7.50

L-Phenylalanine 16.50 16.50 165.00 165.00 16.50 16.50 16.50 16.50 16.50

L-Threonine 24.00 24.00 240.00 240.00 24.00 24.00 24.00 24.00 24.00

L-Tryptophan 4.00 4.00 40.00 40.00 4.00 4.00 4.00 4.00 4.00

L-Tyrosine 18.00 18.00 180.00 180.00 18.00 18.00 18.00 18.00 18.00

L-Valine 23.50 23.50 235.00 235.00 23.50 23.50 23.50 23.50 23.50

Vitamins D(+)-Biotin 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

D-Calciumpantothenate

1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

Choline bitartrate 1.80

Choline chloride 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00

Folic acid 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 20.00 20.00 2.00 2.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 1.00 1.00 0.10 0.10 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 10.00 10.00 1.00 1.00 1.00 1.00 1.00

Weight (g / L) 8.99 9.28 89.87 92.79 14.44 14.74 8.95 9.24 9.17

– 749 –

#250448 APPLICHEM - GENERAL KATALOG 2008 INHALT [749 <749> 778] 26.2.08

General CatalogAppliChem

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Page 23: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

BME, Basal medium with Hanks' saltsComponents A1917 A1313 A1990 A1988 A1918 A1919 A1978

autoclavable

Inorganic salts Calcium chloridex 2H2O

185.44 185.44 1854.44 1854.44 185.44 185.44 185.44

Magnesiumsulfate dried

139.52 139.52 1395.20 1395.20 139.52 139.52 139.52

Potassiumchloride

400.00 400.00 4000.00 4000.00 400.00 400.00 400.00

Potassiumdihydrogenphosphateanhydrous

60.00 60.00 600.00 600.00 60.00 60.00 60.00

Sodium chloride 8000.00 8000.00 80000.00 80000.00 7500.00 7500.00 8000.00

di-Sodiumhydrogenphosphate

47.88 47.88 478.80 478.80 47.88 47.88 47.88

OtherComponents

D(+)-Glucoseanhydrous

1000.00 1000.00 10000.00 10000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00

Phenol red 10.00 10.00 100.00 100.00 10.00 10.00 6.00

Succinic acid 75.00

Succinic acidsodium salt

100.00

Amino acids L-Arginine x HCl 21.00 21.00 210.00 210.00 21.00 21.00 21.00

L-Cystine 12.00 12.00 120.00 120.00 12.00 12.00 12.00

L-Glutamine 0 292.00 0 2920.00 0 292.00 0

L-Histidine Base 8.00 8.00 80.00 80.00 8.00 8.00

L-Histidine x HClx H2O

15.00

L-Isoleucine 26.00 26.00 260.00 260.00 26.00 26.00 26.00

L-Leucine 26.00 26.00 260.00 260.00 26.00 26.00 26.00

L-Lysine x HCl 36.47 36.47 364.70 364.70 36.47 36.47 36.47

L-Methionine 7.50 7.50 75.00 75.00 7.50 7.50 7.50

L-Phenylalanine 16.50 16.50 165.00 165.00 16.50 16.50 16.50

L-Threonine 24.00 24.00 240.00 240.00 24.00 24.00 24.00

L-Tryptophan 4.00 4.00 40.00 40.00 4.00 4.00 4.00

L-Tyrosine 18.00 18.00 180.00 180.00 18.00 18.00 18.00

L-Valine 23.50 23.50 235.00 235.00 23.50 23.50 23.50

Vitamins D(+)-Biotin 1.00 1.00 10.00 10.00 1.00 1.00 1.00

D-Calciumpantothenate

1.00 1.00 10.00 10.00 1.00 1.00 1.00

Choline bitartrate 1.80

Choline chloride 1.00 1.00 10.00 10.00 1.00 1.00

Folic acid 1.00 1.00 10.00 10.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 20.00 20.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 10.00 10.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 10.00 10.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 1.00 1.00 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 10.00 10.00 1.00 1.00 1.00

Weight (g / L) 10.07 10.37 100.75 103.67 15.53 15.82 10.25

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DMEM - MediaComponents A1924 A1314 A1925 A1315 A0826 A1926 A1316 A1985 A1986 A1950 A1965 A1979 A1951 A3134 A3137 A3139

Inorganic salts Calcium chloride x2H2O

264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92

Iron(III)-nitrate x9H2O

0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

Magnesium sulfatedried

139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57

Potassium chloride 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00

Sodium chloride 6400.-00

6400.-00

5900.-00

5900.-00

6400.-00

6400.-00

6400.-00

6400.-00

6400.-00

5400.-00

5400.-00

5400.-00

5400.-00

6400.-00

6400.-00

6400.-00

Sodium dihydrogenphosphate x H2O

125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00

OtherComponents

D(+)-Glucoseanhydrous

1000.-00

1000.-00

1000.-00

1000.-00

1000.-00

4500.-00

4500.-00

4500.-00

4500.-00

4500.-00

4500.-00

4500.-00

4500.-00

1000.-00

4500.-00

4500.-00

HEPES 5958.-00

5958.-00

0 5958.-00

5958.-00

5958.-00

5958.-00

Phenol red 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00

Sodium pyruvate 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00

Amino acids L-Arginine x HCl 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00

L-Cystine 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00

L-Glutamine 0 584.00 0 584.00 0 0 584.00 0 584.00 0 584.00 0 584.00 584.00 0 584.00

Glycine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00

L-Histidine x HCl xH20

42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00

L-Isoleucine 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00

L-Leucine 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00 105.00

L-Lysine x HCl 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00 146.00

L-Methionine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00

L-Phenylalanine 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00

L-Serine 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00

L-Threonine 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00 95.00

L-Tryptophan 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00

L-Tyrosine 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00

L-Valine 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00 94.00

Vitamins D-Calciumpantothenate

4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

Choline chloride 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

Folic acid 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

myo-Inositol 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20

Nicotinamide 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

Pyridoxal x HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

Riboflavin 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40

Thiamine x HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00

Weight (g / L) 9.46 10.05 14.20 15.50 9.45 12.85 13.44 12.96 13.55 17.81 18.39 17.92 18.50 10.03 12.95 13.53

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DMEM / F12 - MediaComponents A1920 A1318 A1922 A1317 A1923 A1921

Inorganic salts Calcium chloride x2H2O

154.45 154.45 154.45 154.45 154.45 154.45

Copper(II)-sulfatex 5H2O

0.0013 0.0013 0.0013 0.0013 0.0013 0.0013

Iron(II)-sulfate x7H2O

0.417 0.417 0.417 0.417 0.417 0.417

Iron(III)-nitrate x9H2O

0.05 0.05 0.05 0.05 0.05 0.05

Magnesiumchloride x 6H2O

61.00 61.00 61.00 61.00 61.00 61.00

Magnesiumsulfate dried

69.76 69.76 69.76 69.76 69.76 69.76

Potassiumchloride

311.80 311.80 311.80 311.80 311.80 311.80

Sodium chloride 6999.50 6999.50 6999.50 6999.50 6999.50 6999.50

Sodiumdihydrogenphosphate x H2O

62.50 62.50 62.50 62.50 62.50 62.50

di-Sodiumhydrogen phos-phate anhydrous

71.02 71.02 71.02 71.02 71.02 71.02

Zinc sulfate x7H2O

0.432 0.432 0.432 0.432 0.432 0.432

OtherComponents

D(+)-Glucoseanhydrous

3151.00 3151.00 3151.00 3151.00 3151.00 3151.00

HEPES 3574.50 3574.50 5958.00 5958.00

Hypoxanthine 2.10 2.10 2.10 2.10 2.10 2.10

Linoleic acid 0.042 0.042 0.042 0.042 0.042 0.042

DL-alpha-Lipoicacid

0.105 0.105 0.105 0.105 0.105 0.105

Phenol red 8.10 8.10 8.10 8.10 8.10 8.10

Putrescine x 2HCl 0.081 0.081 0.081 0.081 0.081 0.081

Sodium pyruvate 55.00 55.00 55.00 55.00 55.00 55.00

Amino acids L-Alanine 4.45 4.45 4.45 4.45 4.45 4.45

L-Arginine x HCl 147.50 147.50 147.50 147.50 147.50 147.50

L-Asparagine xH2O

7.50 7.50 7.50 7.50 7.50 7.50

L-Aspartic acid 6.65 6.65 6.65 6.65 6.65 6.65

L-Cysteine x HCl xH20

17.56 17.56 17.56 17.56 17.56 17.56

L-Cystine 24.00 24.00 24.00 24.00 24.00 24.00

L-Glutamic acidanhydrous

7.35 7.35 7.35 7.35 7.35 7.35

L-Glutamine 0 365.00 0 365.00 0 365.00

Glycine 18.75 18.75 18.75 18.75 18.75 18.75

L-Histidine x HCl xH20

31.48 31.48 31.48 31.48 31.48 31.48

L-Isoleucine 54.47 54.47 54.47 54.47 54.47 54.47

L-Leucine 59.05 59.05 59.05 59.05 59.05 59.05

L-Lysine x HCl 91.25 91.25 91.25 91.25 91.25 91.25

L-Methionine 17.24 17.24 17.24 17.24 17.24 17.24

L-Phenylalanine 35.48 35.48 35.48 35.48 35.48 35.48

L-Proline 17.25 17.25 17.25 17.25 17.25 17.25

L-Serine 26.25 26.25 26.25 26.25 26.25 26.25

L-Threonine 53.45 53.45 53.45 53.45 53.45 53.45

L-Tryptophan 9.02 9.02 9.02 9.02 9.02 9.02

L-Tyrosine 38.70 38.70 38.70 38.70 38.70 38.70

L-Valine 52.85 52.85 52.85 52.85 52.85 52.85

Vitamins D(+)-Biotin 0.0035 0.0035 0.0035 0.0035 0.0035 0.0035

D-Calciumpantothenate

2.24 2.24 2.24 2.24 2.24 2.24

Choline chloride 8.98 8.98 8.98 8.98 8.98 8.98

2'-Deoxythymidine 0.365 0.365 0.365 0.365 0.365 0.365

Folic acid 2.65 2.65 2.65 2.65 2.65 2.65

myo-Inositol 12.60 12.60 12.60 12.60 12.60 12.60

Nicotinamide 2.02 2.02 2.02 2.02 2.02 2.02

Pyridoxal x HCl 2.00 2.00 2.00 2.00 2.00 2.00

Pyridoxine HCl 0.031 0.031 0.031 0.031 0.031 0.031

Riboflavin 0.219 0.219 0.219 0.219 0.219 0.219

Thiamine x HCl 2.17 2.17 2.17 2.17 2.17 2.17

Vitamin B12 0.68 0.68 0.68 0.68 0.68 0.68

Weight (g / L) 11.70 12.07 15.28 15.64 17.66 18.02

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Earle's balanced saltsComponents A1712 A2039 A3026 A3127

Inorganic salts Calcium chloride x 2H2O 264.92 264.92

Magnesium sulfate dried 139.57 139.57

Potassium chloride 400.00 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 6800.00 6800.00

Sodium dihydrogen phosphate xH2O

140.00 140.00 140.00 140.00

OtherComponents

D(+)-Glucose anhydrous 1000.00 1000.00 1000.00 1000.00

Phenol red 10.00 10.00

Weight (g / L) 8.75 8.74 8.35 8.34

Gamborg's B5 Plant saltsComponents A1911

Inorganic salts Ammonium sulfate 134.00

Boric acid 3.00

Calcium chloride x 2H2O 149.98

Cobalt(II)-chloride x 6H2O 0.025

Copper(II)-sulfate x 5H2O 0.025

Iron(II)-sulfate x 7H2O 27.80

Magnesium sulfate dried 172.11

Manganese(II)-sulfate x H2O 10.00

Potassium iodide 0.75

Potassium nitrate 2500.00

Sodium dihydrogen phosphate x H2O 150.00

Sodium molybdate x 2H2O 0.25

Zinc sulfate x 7H2O 2.00

Vitamins D-Calcium pantothenate 0.874

myo-Inositol 100.00

Nicotinamide 1.00

Pyridoxal x HCl 1.00

Riboflavin 0.015

Thiamine x HCl 10.00

Weight (g / L) 3.26

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Glasgow - MEM (BHK 21)Components A2024 A1320 A2025 A1321

Inorganic salts Calcium chloride x 2H2O 264.92 264.92 238.43 238.43

Iron(III)-nitrate x 9H2O 0.10 0.10 0.09 0.09

Magnesium sulfate dried 139.52 139.52 125.64 125.64

Potassium chloride 400.00 400.00 360.00 360.00

Sodium chloride 6400.00 6400.00 6260.00 6260.00

Sodium dihydrogen phosphate xH2O

124.00 124.00 111.60 111.60

di-Sodium hydrogen phosphate 250.00 250.00

OtherComponents

D(+)-Glucose anhydrous 4500.00 4500.00 4250.00 4250.00

Peptone from casein 1000.00 1000.00

Peptone from meat 500.00 500.00

Phenol red 15.00 15.00 13.50 13.50

Yeast extract 500.00 500.00

Amino acids L-Arginine x HCl 42.00 42.00 37.80 37.80

L-Cystine 24.00 24.00 21.60 21.60

L-Glutamine 0 292.00 0 262.80

L-Histidine x HCl x H2O 21.00 21.00 18.90 18.90

L-Isoleucine 52.40 52.40 47.16 47.16

L-Leucine 52.40 52.40 47.16 47.16

L-Lysine x HCl 73.10 73.10 65.79 65.79

L-Methionine 15.00 15.00 13.50 13.50

L-Phenylalanine 33.00 33.00 29.70 29.70

L-Threonine 47.60 47.60 42.84 42.84

L-Tryptophan 8.00 8.00 7.20 7.20

L-Tyrosine 36.20 36.20 32.52 32.52

L-Valine 46.80 46.80 42.12 42.12

Vitamins D-Calcium pantothenate 2.00 2.00 1.80 1.80

Choline chloride 2.00 2.00 1.80 1.80

Folic acid 2.00 2.00 1.80 1.80

myo-Inositol 3.60 3.60 3.24 3.24

Nicotinamide 2.00 2.00 1.80 1.80

Pyridoxal x HCl 2.00 2.00 1.80 1.80

Riboflavin 0.20 0.20 0.18 0.18

Thiamine x HCl 2.00 2.00 1.80 1.80

Weight (g / L) 12.31 12.60 14.03 14.29

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Grace's - Insect mediaComponents A2023 A1529

Inorganic salts Calcium chloride x 2H2O 1324.60 1324.60

Magnesium chloride x 6H2O 2278.86 2278.86

Magnesium sulfate dried 1939.81 1939.81

Potassium chloride 2240.00 2240.00

di-Sodium hydrogen phosphate 876.92 876.92

Other Components D(+)-Glucose anhydrous 700.00 700.00

D(-)-Fructose 400.00 400.00

Fumaric acid 55.00 55.00

a-Ketoglutaric acid sodium salt 425.66 425.66

DL-Malic acid 670.00 670.00

Succinic acid 60.00 60.00

D(+)-Sucrose 26680.00 26680.00

Amino acids L-Alanine 200.00 200.00

L-Arginine x HCl 700.00 700.00

L-Asparagine x H2O 350.00 350.00

L-Aspartic acid 350.00 350.00

L-Cystine 19.18 19.18

L-Glutamic acid anhydrous 600.00 600.00

L-Glutamine 0 600.00

Glycine 650.00 650.00

L-Histidine Base 2500.00 2500.00

L-Isoleucine 50.00 50.00

L-Leucine 75.00 75.00

L-Lysine x HCl 625.00 625.00

L-Methionine 50.00 50.00

L-Phenylalanine 150.00 150.00

L-Proline 350.00 350.00

L-Serine 550.00 550.00

L-Threonine 175.00 175.00

L-Tryptophan 100.00 100.00

L-Tyrosine 50.00 50.00

L-Valine 100.00 100.00

Vitamins p-Aminobenzoic acid 0.02 0.02

D(+)-Biotin 0.01 0.01

D-Calcium pantothenate 0.02 0.02

Choline chloride 0.20 0.20

Folic acid 0.02 0.02

myo-Inositol 0.02 0.02

Nicotinic acid 0.02 0.02

Pyridoxine x HCl 0.02 0.02

Riboflavin 0.02 0.02

Thiamine x HCl 0.02 0.02

Weight (g / L) 45.52 46.12

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Ham's F10 - MediaComponents A1970 A1525 A1989 A1982 A2026 A1952

Inorganic salts Calcium chloride x2H2O

44.09 44.09 44.09 44.09 44.09 44.09

Copper(II)-sulfatex 5H2O

0.0025 0.0025 0.0025 0.0025 0.0025 0.0025

Iron(II)-sulfate x7H2O

0.834 0.834 0.834 0.834 0.834 0.834

Magnesiumsulfate dried

106.06 106.06 106.06 106.06 106.06 106.06

Potassiumchloride

285.00 285.00 285.00 285.00 285.00 285.00

Potassiumdihydrogenphosphate

83.00 83.00 83.00 83.00 83.00 83.00

Sodium chloride 7400.00 7400.00 6900.00 6900.00 6900.00 6900.00

di-Sodiumhydrogenphosphate

153.70 153.70 153.70 153.70 153.70 153.70

Zinc sulfate x7H2O

0.029 0.029 0.029 0.029 0.029 0.029

OtherComponents

D(+)-Glucoseanhydrous

1100.00 1100.00 1100.00 1100.00 1100.00 1100.00

HEPES 5958.00 5958.00 5958.00 5958.00

2'-Deoxythymidine 0.73 0.73 0.73 0.73 0.73 0.73

Hypoxanthine 4.08 4.08 4.08 4.08 4.08 4.08

DL- 0.21 0.21 0.21 0.21 0.21 0.21

Phenol red 1.20 1.20 1.20 1.20 1.20 1.20

Sodium hydrogencarbonate

1200.00 1200.00

Sodium pyruvate 110.00 110.00 110.00 110.00 110.00 110.00

Amino acids L-Alanine 8.91 8.91 8.91 8.91 8.91 8.91

L-Arginine x HCl 211.00 211.00 211.00 211.00 211.00 211.00

L-Asparagine xH2O

15.00 15.00 15.00 15.00 15.00 15.00

L-Aspartic acid 13.30 13.30 13.30 13.30 13.30 13.30

L-Cysteine x HCl xH2O

35.12 35.12 35.12 35.12 35.12 35.12

L-Glutamic acidanhydrous

14.70 14.70 14.70 14.70 14.70 14.70

L-Glutamine 0 146.20 0 146.20 0 146.20

Glycine 7.51 7.51 7.51 7.51 7.51 7.51

L-Histidine x HCl xH2O

21.00 21.00 21.00 21.00 21.00 21.00

L-Isoleucine 2.60 2.60 2.60 2.60 2.60 2.60

L-Leucine 13.10 13.10 13.10 13.10 13.10 13.10

L-Lysine x HCl 29.30 29.30 29.30 29.30 29.30 29.30

L-Methionine 4.48 4.48 4.48 4.48 4.48 4.48

L-Phenylalanine 4.96 4.96 4.96 4.96 4.96 4.96

L-Proline 11.50 11.50 11.50 11.50 11.50 11.50

L-Serine 10.50 10.50 10.50 10.50 10.50 10.50

L-Threonine 3.57 3.57 3.57 3.57 3.57 3.57

L-Tryptophan 0.60 0.60 0.60 0.60 0.60 0.60

L-Tyrosine 1.81 1.81 1.81 1.81 1.81 1.81

L-Valine 3.50 3.50 3.50 3.50 3.50 3.50

Vitamins D(+)-Biotin 0.024 0.024 0.024 0.024 0.024 0.024

D-Calciumpantothenate

0.715 0.715 0.715 0.715 0.715 0.715

Choline chloride 0.698 0.698 0.698 0.698 0.698 0.698

Folic acid 1.32 1.32 1.32 1.32 1.32 1.32

myo-Inositol 0.541 0.541 0.541 0.541 0.541 0.541

Nicotinamide 0.615 0.615 0.615 0.615 0.615 0.615

Pyridoxine x HCl 0.21 0.21 0.21 0.21 0.21 0.21

Riboflavin 0.376 0.376 0.376 0.376 0.376 0.376

Thiamine x HCl 1.01 1.01 1.01 1.01 1.01 1.01

Vitamin B12 1.36 1.36 1.36 1.36 1.36 1.36

Weight (g / L) 9.71 9.85 15.17 15.31 16.37 16.51

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Ham's F12 - MediaComponents A1948 A1526 A1969 A1953

Inorganic salts Calcium chloride x 2H2O 44.00 44.00 44.00 44.00

Copper(II)-sulfate x 5H2O 0.003 0.003 0.003 0.003

Iron(II)-sulfate x 7H2O 0.834 0.834 0.834 0.834

Magnesium chloride x 6H2O 122.00 122.00 122.00 122.00

Potassium chloride 223.60 223.60 223.60 223.60

Sodium chloride 7599.00 7599.00 7099.00 7099.00

di-Sodium hydrogen phosphate 142.04 142.04 142.04 142.04

Zinc sulfate x 7H2O 0.863 0.863 0.863 0.863

OtherComponents

D(+)-Glucose anhydrous 1801.60 1801.60 1801.60 1801.60

HEPES 5958.00 5958.00

2'-Deoxythymidine 0.73 0.73 0.73 0.73

Hypoxanthine 4.08 4.08 4.08 4.08

Linoleic acid 0.084 0.084 0.084 0.084

DL-a-Lipoic acid 0.21 0.21 0.21 0.21

Phenol red 1.20 1.20 1.20 1.20

Putrescine x 2HCl 0.161 0.161 0.161 0.161

Sodium pyruvate 110.10 110.10 110.10 110.10

Amino acids L-Alanine 8.91 8.91 8.91 8.91

L-Arginine x HCl 210.70 210.70 210.70 210.70

L-Asparagine x H2O 15.01 15.01 15.01 15.01

L-Aspartic acid 13.31 13.31 13.31 13.31

L-Cysteine x HCl x H2O 35.12 35.12 35.12 35.12

L-Glutamic acid anhydrous 14.71 14.71 14.71 14.71

L-Glutamine 0 146.20 0 146.20

Glycine 7.51 7.51 7.51 7.51

L-Histidine x HCl x H2O 20.96 20.96 20.96 20.96

L-Isoleucine 3.94 3.94 3.94 3.94

L-Leucine 13.12 13.12 13.12 13.12

L-Lysine x HCl 36.54 36.54 36.54 36.54

L-Methionine 4.48 4.48 4.48 4.48

L-Phenylalanine 4.96 4.96 4.96 4.96

L-Proline 34.53 34.53 34.53 34.53

L-Serine 10.51 10.51 10.51 10.51

L-Threonine 11.91 11.91 11.91 11.91

L-Tryptophan 2.04 2.04 2.04 2.04

L-Tyrosine 5.44 5.44 5.44 5.44

L-Valine 11.71 11.71 11.71 11.71

Vitamins D(+)-Biotin 0.007 0.007 0.007 0.007

D-Calcium pantothenate 0.24 0.24 0.24 0.24

Choline chloride 13.96 13.96 13.96 13.96

Folic acid 1.32 1.32 1.32 1.32

myo-Inositol 18.02 18.02 18.02 18.02

Nicotinamide 0.037 0.037 0.037 0.037

Pyridoxine x HCl 0.062 0.062 0.062 0.062

Riboflavin 0.038 0.038 0.038 0.038

Thiamine x HCl 0.34 0.34 0.34 0.34

Vitamin B12 1.36 1.36 1.36 1.36

Weight (g / L) 10.55 10.70 16.01 16.16

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Ham's F12K - MediaComponents A1963 A1927

Inorganic salts Calcium chloride x 2H2O 135.24 135.24

Copper(II)-sulfate x 5H2O 0.0025 0.0025

Iron(II)-sulfate x 7H2O 0.834 0.834

Magnesium chloride x 6H2O 105.72 105.72

Magnesium sulfate dried 275.20 275.20

Potassium chloride 283.29 283.29

Potassium dihydrogen phosphate 58.52 58.52

Sodium chloride 7597.20 7597.20

di-Sodium hydrogen phosphate 115.02 115.02

Zinc sulfate x 7H2O 0.1437 0.1437

Other Components D(+)-Glucose anhydrous 1260.00 1260.00

2'-Deoxythymidine 0.7266 0.7266

Hypoxanthine 4.08 4.08

DL-a-Lipoic acid 0.2063 0.2063

Phenol red 3.00 3.00

Putrescine x 2HCl 0.3222 0.3222

Sodium pyruvate 220.00 220.00

Amino acids L-Alanine 17.82 17.82

L-Arginine x HCl 421.40 421.40

L-Asparagine x H2O 30.02 30.02

L-Aspartic acid 26.62 26.62

L-Cysteine x HCl x H2O 70.24 70.24

L-Glutamic acid anhydrous 29.42 29.42

L-Glutamine 0 292.20

Glycine 15.01 15.01

L-Histidine x HCl x H2O 41.92 41.92

L-Isoleucine 7.87 7.87

L-Leucine 26.24 26.24

L-Lysine x HCl 73.04 73.04

L-Methionine 8.95 8.95

L-Phenylalanine 9.91 9.91

L-Proline 69.06 69.06

L-Serine 21.02 21.02

L-Threonine 23.82 23.82

L-Tryptophan 4.08 4.08

L-Tyrosine 10.87 10.87

L-Valine 23.42 23.42

Vitamins D(+)-Biotin 0.0732 0.0732

D-Calcium pantothenate 0.477 0.477

Choline chloride 13.96 13.96

Folic acid 1.3242 1.32

myo-Inositol 18.02 18.02

Nicotinamide 0.0366 0.0366

Pyridoxine x HCl 0.0617 0.0617

Riboflavin 0.0376 0.0376

Thiamine x HCl 0.3373 0.3373

Vitamin B12 1.3554 1.3554

Weight (g / L) 11.03 11.32

Hanks' balanced saltsComponents A1711 A3140 A2729 A2512

Inorganic salts Calcium chloride x 2H2O 185.44 185.44

Magnesium sulfate dried 139.52 139.52

Potassium chloride 400.00 400.00 400.00 400.00

Potassium dihydrogenphosphate

60.00 60.00 60.00 60.00

Sodium chloride 8000.00 8000.00 8000.00 8000.00

di-Sodium hydrogen phosphateanhydrous

47.88 47.88 47.88 47.88

OtherComponents

D(+)-Glucose anhydrous 1000.00 1000.00 1000.00 1000.00

Phenol red 10.00 10.00

Weight (g / L) 9.84 9.83 9.52 9.51

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IPL-41 - MediaComponents A2027 A1964

Inorganic salts Ammonium heptamolybdate x 4H2O 0.04 0.04

Calcium chloride x 2H2O 662.30 662.30

Cobalt(II)-chloride x 6H2O 0.05 0.05

Cupric chloride x 6H2O 0.20 0.20

Iron(II)-sulfate x 7H2O 0.55 0.55

Magnesium sulfate dried 1311.40 1311.40

Manganese chloride x 4H2O 0.02 0.02

Potassium chloride 1200.00 1200.00

Sodium chloride 2850.00 2850.00

Sodium dihydrogen phosphate x H2O 1160.00 1160.00

Tin chloride 0.04 0.04

Other Components D(+)-Glucose anhydrous 2500.00 2500.00

Fumaric acid 4.40 4.40

a-Ketoglutaric acid sodium salt 34.05 34.05

DL-Malic acid 53.60 53.60

D(+)-Maltose x H2O 1052.58 1052.58

Succinic acid 4.80 4.80

D(+)-Sucrose 1650.00 1650.00

Amino acids b-Alanine 300.00 300.00

L-Arginine x HCl 800.00 800.00

L-Asparagine x H2O 1477.14 1477.14

L-Aspartic acid 1300.00 1300.00

L-Cystine 100.00 100.00

L-Glutamic acid anhydrous 1500.00 1500.00

L-Glutamine 0 1000.00

Glycine 200.00 200.00

L-Histidine Base 200.00 200.00

L-Hydroxyproline 800.00 800.00

L-Isoleucine 750.00 750.00

L-Leucine 250.00 250.00

L-Lysine x HCl 700.00 700.00

L-Methionine 1000.00 1000.00

L-Phenylalanine 1000.00 1000.00

L-Proline 500.00 500.00

L-Serine 200.00 200.00

L-Threonine 200.00 200.00

L-Tryptophan 100.00 100.00

L-Tyrosine 250.02 250.02

L-Valine 500.00 500.00

Vitamins p-Aminobenzoic acid 0.32 0.32

D(+)-Biotin 0.16 0.16

D-Calcium pantothenate 0.008 0.008

Choline chloride 20.00 20.00

Folic acid 0.08 0.08

myo-Inositol 0.40 0.40

Nicotinamide 0.16 0.16

Nicotinic acid 0.16 0.16

Pyridoxine x HCl 0.40 0.40

Riboflavin 0.08 0.08

Thiamine x HCl 0.08 0.08

Vitamin B12 0.24 0.24

Weight (g / L) 24.63 25.63

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Iscove's modified Dulbecco's - Media (IMDM)Components A2028 A1909 A2029 A1322

Inorganic salts Calcium chloride x 2H2O 218.56 218.56 218.56 218.56

Magnesium sulfate dried 139.52 139.52 139.52 139.52

Potassium chloride 330.00 330.00 330.00 330.00

Potassium nitrate 0.0760 0.0760 0.0760 0.0760

Sodium chloride 5005.00 5005.00 4505.00 4505.00

Sodium dihydrogen phosphate xH2O

125.00 125.00 125.00 125.00

Sodium selenite anhydrous 0.0110 0.0110 0.0110 0.0110

OtherComponents

D(+)-Glucose anhydrous 4500.00 4500.00 4500.00 4500.00

HEPES 5958.00 5958.00

Phenol red 15.00 15.00 15.00 15.00

Sodium pyruvate 110.00 110.00 110.00 110.00

Amino acids L-Alanine 25.00 25.00 25.00 25.00

L-Arginine x HCl 84.00 84.00 84.00 84.00

L-Asparagine x H2O 28.40 28.40 28.40 28.40

L-Aspartic acid 30.00 30.00 30.00 30.00

L-Cystine 70.00 70.00 70.00 70.00

L-Glutamic acid anhydrous 75.00 75.00 75.00 75.00

L-Glutamine 0 584.00 0 584.00

Glycine 30.00 30.00 30.00 30.00

L-Histidine x HCl x H2O 42.00 42.00 42.00 42.00

L-Isoleucine 105.00 105.00 105.00 105.00

L-Leucine 105.00 105.00 105.00 105.00

L-Lysine x HCl 146.00 146.00 146.00 146.00

L-Methionine 30.00 30.00 30.00 30.00

L-Phenylalanine 66.00 66.00 66.00 66.00

L-Proline 40.00 40.00 40.00 40.00

L-Serine 42.00 42.00 42.00 42.00

L-Threonine 95.00 95.00 95.00 95.00

L-Tryptophan 16.00 16.00 16.00 16.00

L-Tyrosine 74.86 74.86 74.86 74.86

L-Valine 94.00 94.00 94.00 94.00

Vitamins D(+)-Biotin 0.0130 0.0130 0.0130 0.0130

D-Calcium pantothenate 4.00 4.00 4.00 4.00

Choline chloride 4.00 4.00 4.00 4.00

Folic acid 4.00 4.00 4.00 4.00

myo-Inositol 7.20 7.20 7.20 7.20

Nicotinamide 4.00 4.00 4.00 4.00

Pyridoxal x HCl 4.00 4.00 4.00 4.00

Riboflavin 0.40 0.40 0.40 0.40

Thiamine x HCl 4.00 4.00 4.00 4.00

Vitamin B12 0.0130 0.0130 0.0130 0.0130

Weight (g / L) 11.67 12.26 17.13 17.72

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Page 34: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Joklik-MEM, modified for spinner cultureComponents A1974 A1539

Inorganic salts Magnesium chloride x 6H2O 200.00 200.00

Potassium chloride 400.00 400.00

Sodium chloride 6500.00 6500.00

Sodium dihydrogen phosphate x H2O 1327.00 1327.00

Other Components D(+)-Glucose anhydrous 2000.00 2000.00

Phenol red 10.00 10.00

Amino acids L-Arginine x HCl 126.00 126.00

L-Cystine 24.00 24.00

L-Glutamine 0 294.00

L-Histidine Base 31.00 31.00

L-Isoleucine 52.00 52.00

L-Leucine 52.00 52.00

L-Lysine x HCl 65.00 65.00

L-Methionine 15.00 15.00

L-Phenylalanine 32.00 32.00

L-Threonine 48.00 48.00

L-Tryptophan 10.00 10.00

L-Tyrosine 32.60 32.60

L-Valine 46.00 46.00

Vitamins D-Calcium pantothenate 1.00 1.00

Choline chloride 1.00 1.00

Folic acid 1.00 1.00

myo-Inositol 2.00 2.00

Nicotinamide 1.00 1.00

Pyridoxal x HCl 1.00 1.00

Riboflavin 0.10 0.10

Thiamine x HCl 1.00 1.00

Weight (g / L) 10.98 11.27

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Leibovitz's L15 - MediaComponents A1967 A1323 A1968 A1954

Inorganic salts Calcium chloride x 2H2O 185.44 185.44 185.44 185.44

Magnesium chloride x 6H2O 200.00 200.00 200.00 200.00

Magnesium sulfate dried 139.52 139.52 139.52 139.52

Potassium chloride 400.00 400.00 400.00 400.00

Potassium dihydrogenphosphate

60.00 60.00 60.00 60.00

Sodium chloride 8000.00 8000.00 7500.00 7500.00

di-Sodium hydrogen phosphate 190.00 190.00 190.00 190.00

OtherComponents

HEPES 5958.00 5958.00

D(+)-Galactose anhydrous 900.00 900.00 900.00 900.00

Phenol red 10.00 10.00 10.00 10.00

Sodium pyruvate 550.00 550.00 550.00 550.00

Amino acids L-Alanine 225.00 225.00 225.00 225.00

L-Arginine Base 500.00 500.00 500.00 500.00

L-Asparagine x H2O 250.00 250.00 250.00 250.00

L-Cysteine 120.00 120.00 120.00 120.00

L-Glutamine 0 300.00 0 300.00

Glycine 200.00 200.00 200.00 200.00

L-Histidine Base 250.00 250.00 250.00 250.00

L-Isoleucine 250.00 250.00 250.00 250.00

L-Leucine 125.00 125.00 125.00 125.00

L-Lysine x HCl 93.75 93.75 93.75 93.75

L-Methionine 75.00 75.00 75.00 75.00

L-Phenylalanine 125.00 125.00 125.00 125.00

L-Serine 200.00 200.00 200.00 200.00

L-Threonine 300.00 300.00 300.00 300.00

L-Tryptophan 20.00 20.00 20.00 20.00

L-Tyrosine 300.00 300.00 300.00 300.00

L-Valine 100.00 100.00 100.00 100.00

Vitamins D-Calcium pantothenate 1.00 1.00 1.00 1.00

Choline chloride 1.00 1.00 1.00 1.00

Folic acid 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 1.00 1.00

Pyridoxine x HCl 1.00 1.00 1.00 1.00

Riboflavin-5'-phosphate sodiumsalt x H2O

0.1075 0.1075 0.1075 0.1075

Thiamine monophosphatechloride

1.00 1.00 1.00 1.00

Weight (g / L) 13.78 14.08 19.23 19.53

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Linsmaier, Bednar & Skoog Plant saltsComponents A0591

Inorganic salts Ammonium nitrate 1650.00

Boric acid 6.20

Calcium chloride x 2H2O 439.98

Cobalt(II)-chloride x 6H2O 0.025

Copper(II)-sulfate x 5H2O 0.025

EDTA disodiumsalt x 2H2O 37.25

Iron(II)-sulfate x 7H2O 27.85

Magnesium sulfate dried 258.37

Manganese(II)-sulfate x H2O 16.90

Potassium dihydrogen phosphate 170.00

Potassium iodide 0.83

Potassium nitrate 1900.00

Sodium molybdate x 2H2O 0.25

Zinc sulfate x 7H2O 10.58

Vitamins myo-Inositol 100.00

Thiamine x HCl 0.40

Weight (g / L) 4.62

McCoy's 5A - MediaComponents A2034 A1324 A2035 A1955

Inorganic salts Calcium chloride x 2H2O 132.46 132.46 132.46 132.46

Magnesium sulfate dried 139.52 139.52 139.52 139.52

Potassium chloride 400.00 400.00 400.00 400.00

Sodium chloride 6460.00 6460.00 5960.00 5960.00

Sodium dihydrogen phosphate xH2O

580.00 580.00 580.00 580.00

OtherComponents

D(+)-Glucose anhydrous 3000.00 3000.00 3000.00 3000.00

HEPES 5960.00 5960.00

Bacto - Peptone 600.00 600.00 600.00 600.00

Glutathione (red,) 0.50 0.50 0.50 0.50

Phenol red 10.00 10.00 10.00 10.00

Amino acids L-Alanine 13.36 13.36 13.36 13.36

L-Arginine x HCl 42.10 42.10 42.10 42.10

L-Asparagine x H2O 45.00 45.00 45.00 45.00

L-Aspartic acid 19.97 19.97 19.97 19.97

L-Cysteine 24.24 24.24 24.24 24.24

L-Glutamic acid 22.10 22.10 22.10 22.10

L-Glutamine 0 219.20 0 219.20

Glycine 7.50 7.50 7.50 7.50

L-Histidine x HCl x H2O 20.76 20.76 20.76 20.76

L-Hydroxyproline 19.70 19.70 19.70 19.70

L-Isoleucine 39.36 39.36 39.36 39.36

L-Leucine 39.36 39.36 39.36 39.36

L-Lysine x HCl 36.50 36.50 36.50 36.50

L-Methionine 14.90 14.90 14.90 14.90

L-Phenylalanine 16.50 16.50 16.50 16.50

L-Proline 17.30 17.30 17.30 17.30

L-Serine 26.30 26.30 26.30 26.30

L-Threonine 17.90 17.90 17.90 17.90

L-Tryptophan 3.10 3.10 3.10 3.10

L-Tyrosine 18.10 18.10 18.10 18.10

L-Valine 17.60 17.60 17.60 17.60

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McCoy's 5A - MediaComponents A2034 A1324 A2035 A1955

Vitamins p-Aminobenzoic acid 1.00 1.00 1.00 1.00

Ascorbic acid 0.50 0.50 0.50 0.50

D(+)-Biotin 0.20 0.20 0.20 0.20

D-Calcium pantothenate 0.20 0.20 0.20 0.20

Choline chloride 5.00 5.00 5.00 5.00

Folic acid 10.00 10.00 10.00 10.00

myo-Inositol 36.00 36.00 36.00 36.00

Nicotinamide 0.50 0.50 0.50 0.50

Nicotinic acid 0.50 0.50 0.50 0.50

Pyridoxal x HCl 0.50 0.50 0.50 0.50

Pyridoxine x HCl 0.50 0.50 0.50 0.50

Riboflavin 0.20 0.20 0.20 0.20

Thiamine x HCl 0.20 0.20 0.20 0.20

Vitamin B12 2.00 2.00 2.00 2.00

Weight (g / L) 11.84 12.06 17.30 17.52

Medium 199 with Earle's saltsComponents A2022 A1325 A2030 A1326

Inorganic salts Calcium chloride x 2H2O 264.92 264.92 264.92 264.92

Iron(III)-nitrate x 9H2O 0.72 0.72 0.72 0.72

Magnesium sulfate dried 139.52 139.52 139.52 139.52

Potassium chloride 400.00 400.00 400.00 400.00

Sodium acetate x 3H2O 82.95 82.95 82.95 82.95

Sodium chloride 6800.00 6800.00 6300.00 6300.00

Sodium dihydrogen phosphate 140.00 140.00 140.00 140.00

OtherComponents

D(+)-Glucose anhydrous 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00

AMP 0.20 0.20 0.20 0.20

ATP 1.00 1.00 1.00 1.00

Adenine sulfate 10.00 10.00 10.00 10.00

Cholesterol 0.20 0.20 0.20 0.20

2-Deoxy-D-ribose 0.50 0.50 0.50 0.50

Glutathione (red.) 0.05 0.05 0.05 0.05

Guanine x HCl 0.30 0.30 0.30 0.30

Hypoxanthine 0.30 0.30 0.30 0.30

Phenol red 10.00 10.00 10.00 10.00

D-Ribose 0.50 0.50 0.50 0.50

Thymine 0.30 0.30 0.30 0.30

Tween 80 4.90 4.90 4.90 4.90

Uracil 0.30 0.30 0.30 0.30

Xanthine 0.30 0.30 0.30 0.30

Amino acids L-Alanine 25.00 25.00 25.00 25.00

L-Arginine x HCl 70.00 70.00 70.00 70.00

L-Aspartic acid 30.00 30.00 30.00 30.00

L-Cysteine x HCl x H2O 0.10 0.10 0.10 0.10

L-Cystine 20.00 20.00 20.00 20.00

L-Glutamic acid anhydrous 67.00 67.00 67.00 67.00

L-Glutamine 0 100.00 0 100.00

Glycine 50.00 50.00 50.00 50.00

L-Histidine x HCl x H2O 21.88 21.88 21.88 21.88

L-Hydroxyproline 10.00 10.00 10.00 10.00

L-Isoleucine 20.00 20.00 20.00 20.00

L-Leucine 60.00 60.00 60.00 60.00

L-Lysine x HCl 70.00 70.00 70.00 70.00

L-Methionine 15.00 15.00 15.00 15.00

L-Phenylalanine 25.00 25.00 25.00 25.00

L-Proline 40.00 40.00 40.00 40.00

L-Serine 25.00 25.00 25.00 25.00

L-Threonine 30.00 30.00 30.00 30.00

L-Tryptophan 10.00 10.00 10.00 10.00

L-Tyrosine 40.00 40.00 40.00 40.00

L-Valine 25.00 25.00 25.00 25.00

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Medium 199 with Earle's saltsComponents A2022 A1325 A2030 A1326

Vitamins p-Aminobenzoic acid 0.05 0.05 0.05 0.05

Ascorbic acid 0.05 0.05 0.05 0.05

D(+)-Biotin 0.01 0.01 0.01 0.01

Calciferol 0.10 0.10 0.10 0.10

D-Calcium pantothenate 0.01 0.01 0.01 0.01

Choline chloride 0.50 0.50 0.50 0.50

Folic acid 0.01 0.01 0.01 0.01

myo-Inositol 0.05 0.05 0.05 0.05

Menadione 0.01 0.01 0.01 0.01

Nicotinamide 0.025 0.025 0.025 0.025

Nicotinic acid 0.025 0.025 0.025 0.025

Pyridoxal x HCl 0.025 0.025 0.025 0.025

Pyridoxine x HCl 0.025 0.025 0.025 0.025

Riboflavin 0.01 0.01 0.01 0.01

Thiamine x HCl 0.01 0.01 0.01 0.01

DL-a-Tocopherolphosphate-Na2 0.01 0.01 0.01 0.01

Vitamin A acetate 0.14 0.14 0.14 0.14

Weight (g / L) 9.51 9.61 14.97 15.07

Medium 199 with Hanks' saltsComponents A2031 A1327 A2021 A1328

Inorganic salts Calcium chloride x 2H2O 185.44 185.44 185.44 185.44

Iron(III)-nitrate x 9H2O 0.72 0.72 0.72 0.72

Magnesium sulfate dried 139.68 139.68 139.68 139.68

Potassium chloride 400.00 400.00 400.00 400.00

Potassium dihydrogenphosphate

60.00 60.00 60.00 60.00

Sodium acetate x 3H2O 83.00 83.00 83.00 83.00

Sodium chloride 8000.00 8000.00 7500.00 7500.00

di-Sodium hydrogen phosphate 47.68 47.68 47.68 47.68

OtherComponents

D(+)-Glucose anhydrous 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00

AMP 0.20 0.20 0.20 0.20

ATP 1.00 1.00 1.00 1.00

Adenine sulfate 10.00 10.00 10.00 10.00

Cholesterol 0.20 0.20 0.20 0.20

2-Deoxy-D-ribose 0.50 0.50 0.50 0.50

Glutathione (red.) 0.05 0.05 0.05 0.05

Guanine x HCl 0.30 0.30 0.30 0.30

Hypoxanthine 0.30 0.30 0.30 0.30

Phenol red 10.00 10.00 10.00 10.00

D-Ribose 0.50 0.50 0.50 0.50

Thymine 0.30 0.30 0.30 0.30

Tween 80 4.90 4.90 4.90 4.90

Uracil 0.30 0.30 0.30 0.30

Xanthine 0.30 0.30 0.30 0.30

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#250448 APPLICHEM - GENERAL KATALOG 2008 INHALT [749 <765> 778] 26.2.08

General CatalogAppliChem

Page 39: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Medium 199 with Hanks' saltsComponents A2031 A1327 A2021 A1328

Amino acids L-Alanine 25.00 25.00 25.00 25.00

L-Arginine x HCl 70.00 70.00 70.00 70.00

L-Aspartic acid 30.00 30.00 30.00 30.00

L-Cysteine x HCl x H2O 0.10 0.10 0.10 0.10

L-Cystine 20.00 20.00 20.00 20.00

L-Glutamic acid anhydrous 67.00 67.00 67.00 67.00

L-Glutamine 0 100.00 0 100.00

Glycine 50.00 50.00 50.00 50.00

L-Histidine x HCl x H2O 21.88 21.88 21.88 21.88

L-Hydroxyproline 10.00 10.00 10.00 10.00

L-Isoleucine 20.00 20.00 20.00 20.00

L-Leucine 60.00 60.00 60.00 60.00

L-Lysine x HCl 70.00 70.00 70.00 70.00

L-Methionine 15.00 15.00 15.00 15.00

L-Phenylalanine 25.00 25.00 25.00 25.00

L-Proline 40.00 40.00 40.00 40.00

L-Serine 25.00 25.00 25.00 25.00

L-Threonine 30.00 30.00 30.00 30.00

L-Tryptophan 10.00 10.00 10.00 10.00

L-Tyrosine 40.00 40.00 40.00 40.00

L-Valine 25.00 25.00 25.00 25.00

Vitamins p-Aminobenzoic acid 0.05 0.05 0.05 0.05

Ascorbic acid 0.05 0.05 0.05 0.05

D(+)-Biotin 0.01 0.01 0.01 0.01

Calciferol 0.10 0.10 0.10 0.10

D-Calcium pantothenate 0.01 0.01 0.01 0.01

Choline chloride 0.50 0.50 0.50 0.50

Folic acid 0.01 0.01 0.01 0.01

myo-Inositol 0.05 0.05 0.05 0.05

Menadione 0.01 0.01 0.01 0.01

Nicotinamide 0.025 0.025 0.025 0.025

Nicotinic acid 0.025 0.025 0.025 0.025

Pyridoxal x HCl 0.025 0.025 0.025 0.025

Pyridoxine x HCl 0.025 0.025 0.025 0.025

Riboflavin 0.01 0.01 0.01 0.01

Thiamine x HCl 0.01 0.01 0.01 0.01

DL-a-Tocopherolphosphate-Na2 0.01 0.01 0.01 0.01

Vitamin A acetate 0.14 0.14 0.14 0.14

Weight (g / L) 10.60 10.70 16.06 16.16

a-MEMComponents A1971 A1330 A1913 A1329 A1980 A1972 A1949 A1914

Inorganic salts Calcium chloridex 2H2O

264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92

Magnesiumsulfate dried

139.52 139.52 139.52 139.52 139.52 139.52 139.52 139.52

Potassiumchloride

400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 6800.00 6800.00 6300.00 6300.00 6300.00 6300.00

Sodiumdihydrogenphosphate x H2O

140.00 140.00 140.00 140.00 140.00 140.00 140.00 140.00

OtherComponents

D(+)-Glucoseanhydrous

1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00 5958.00 5958.00

Lipoic acid 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20

Phenol red 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00

Sodium pyruvate 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00

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Page 40: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

a-MEMComponents A1971 A1330 A1913 A1329 A1980 A1972 A1949 A1914

Amino acids L-Alanine 25.00 25.00 25.00 25.00 25.00 25.00 25.00 25.00

L-Arginine x HCl 126.64 126.64 126.64 126.64 126.64 126.64 126.64 126.64

L-Asparagine xH2O

50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00

L-Aspartic acid 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00

L-Cysteine x HClx H2O

100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00

L-Cystine 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00

L-Glutamic acidanhydrous

75.00 75.00 75.00 75.00 75.00 75.00 75.00 75.00

L-Glutamine 0 292.00 0 292.00 0 292.00 0 292.00

L-Glycine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00

L-Histidine x HClx H2O

42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00

L-Isoleucine 52.40 52.40 52.40 52.40 52.40 52.40 52.40 52.40

L-Leucine 52.40 52.40 52.40 52.40 52.40 52.40 52.40 52.40

L-Lysine x HCl 72.47 72.47 72.47 72.47 72.47 72.47 72.47 72.47

L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00

L-Phenylalanine 32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00

L-Proline 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00

L-Serine 25.00 25.00 25.00 25.00 25.00 25.00 25.00 25.00

L-Threonine 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00

L-Tryptophan 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00

L-Tyrosine 36.20 36.20 36.20 36.20 36.20 36.20 36.20 36.20

L-Valine 46.00 46.00 46.00 46.00 46.00 46.00 46.00 46.00

Vitamins L-Ascorbic acid 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00

D(+)-Biotin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

D-Calciumpantothenate

1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Choline chloride 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Folic acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Vitamin B12 1.33 1.33 1.33 1.33 1.33 1.33 1.33 1.33

Ribonucleo-sides

Adenosine 10.00 10.00 10.00 10.00

Cytidine 10.00 10.00 10.00 10.00

Guanosine 10.00 10.00 10.00 10.00

Uridine 10.00 10.00 10.00 10.00

Deoxyribonu-cleosides

2'-Deoxyadeno-sine x H2O

10.00 10.00 10.00 10.00

2'-Deoxycytidinex HCl

11.00 11.00 10.00 10.00

2'-Deoxyguano-sine

10.00 10.00 10.00 10.00

2'-Deoxythymi-dine

10.00 10.00 10.00 10.00

Weight (g / L) 9.88 10.17 9.96 10.25 15.33 15.63 15.41 15.71

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Page 41: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

MEM with Earle's saltsComponents A1331 A1332 A2018 A1334 A2019 A1333 A2032 A1956 A2597

autoclava-ble

Inorganic salts Calcium chloridex 2H2O

264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92

Magnesiumsulfate dried

139.52 139.52 139.52 139.52 139.52 139.52 139.52 139.52 139.52

Potassiumchloride

400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 6800.00 6800.00 6300.00 6300.00 6300.00 6300.00 6800.00

Sodiumdihydrogenphosphate x H2O

140.00 140.00 140.00 140.00 140.00 140.00 140.00 140.00 140.00

OtherComponents

D(+)-Glucoseanhydrous

1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00 5958.00 5958.00

Phenol red 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 6.00

Succinic acid 75.00

Succinic acidsodium salt

100.00

Amino acids L-Alanine 8.90 8.90 8.90 8.90

L-Arginine x HCl 126.00 126.00 126.00 126.00 126.00 126.00 126.00 126.00 126.00

L-Asparagine xH2O

13.20 13.20 13.20 13.20

L-Aspartic acid 13.30 13.30 13.30 13.30

L-Cystine 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00

L-Glutamic acidanhydrous

14.70 14.70 14.70 14.70

L-Glutamine 0 292.00 0 292.00 0 292.00 0 292.00 0

Glycine 7.50 7.50 7.50 7.50

L-Histidine x HClx H2O

42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00

L-Isoleucine 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00

L-Leucine 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00

L-Lysine x HCl 72.50 72.50 72.50 72.50 72.50 72.50 72.50 72.50 72.50

L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00

L-Phenylalanine 32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00

L-Proline 11.50 11.50 11.50 11.50

L-Serine 10.50 10.50 10.50 10.50

L-Threonine 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00

L-Tryptophan 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00

L-Tyrosine 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00

L-Valine 46.00 46.00 46.00 46.00 46.00 46.00 46.00 46.00 46.00

Vitamins D-Calciumpantothenate

1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Choline bitartrate 1.80

Choline chloride 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Folic acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Weight (g / L) 9.32 9.61 9.40 9.69 14.78 15.07 14.86 15.15 9.49

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Page 42: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

MEM with Hanks' saltsComponents A1957 A1335 A2041 A1337 A2042 A1336 A2043 A1958

Inorganic salts Calcium chloridex 2H2O

185.44 185.44 185.44 185.44 185.44 185.44 185.44 185.44

Magnesiumsulfate dried

139.52 139.52 139.52 139.52 139.52 139.52 139.52 139.52

Potassiumchloride

400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00

Potassiumdihydrogenphosphateanhydrous

60.00 60.00 60.00 60.00 60.00 60.00 60.00 60.00

Sodium chloride 8000.00 8000.00 8000.00 8000.00 7500.00 7500.00 7500.00 7500.00

di-Sodiumhydrogenphosphate

47.88 47.88 47.88 47.88 47.88 47.88 47.88 47.88

OtherComponents

D(+)-Glucoseanhydrous

1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00

HEPES 5958.00 5958.00 5958.00 5958.00

Phenol red 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00

Amino acids L-Alanine 8.90 8.90 8.90 8.90

L-Arginine x HCl 126.00 126.00 126.00 126.00 126.00 126.00 126.00 126.00

L-Asparagine xH2O

13.20 13.20 13.20 13.20

L-Aspartic acid 13.30 13.30 13.30 13.30

L-Cystine 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00

L-Glutamic acidanhydrous

14.70 14.70 14.70 14.70

L-Glutamine 0 292.00 0 292.00 0 292.00 0 292.00

Glycine 7.50 7.50 7.50 7.50

L-Histidine x HClx H2O

42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00

L-Isoleucine 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00

L-Leucine 52.00 52.00 52.00 52.00 52.00 52.00 52.00 52.00

L-Lysine x HCl 72.50 72.50 72.50 72.50 72.50 72.50 72.50 72.50

L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00

L-Phenylalanine 32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00

L-Proline 11.50 11.50 11.50 11.50

L-Serine 10.50 10.50 10.50 10.50

L-Threonine 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00

L-Tryptophan 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00

L-Tyrosine 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00

L-Valine 46.00 46.00 46.00 46.00 46.00 46.00 46.00 46.00

Vitamins D-Calciumpantothenate

1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Choline chloride 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Folic acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00

Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00

Weight (g / L) 10.41 10.70 10.49 10.78 15.86 16.16 15.94 16.24

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General CatalogAppliChem

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S-MEM, modified for spinner cultureComponents A1338 A1308 A1975

autoclavable

Inorganic salts Magnesium sulfate dried 139.52 139.52 139.52

Potassium chloride 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 6800.00

Sodium dihydrogen phosphate xH2O

1400.00 1400.00 1400.00

OtherComponents

D(+)-Glucose anhydrous 1000.00 1000.00 1000.00

Phenol red 10.00 10.00 6.00

Succinic acid 75.00

Succinic acid sodium salt 100.00

Amino acids L-Arginine x HCl 126.00 126.00 126.00

L-Cystine 24.00 24.00 24.00

L-Glutamine 0 292.00 0

L-Histidine x HCl 42.00 42.00 42.00

L-Isoleucine 52.00 52.00 52.00

L-Leucine 52.00 52.00 52.00

L-Lysine x HCl 72.50 72.50 72.50

L-Methionine 15.00 15.00 15.00

L-Phenylalanine 32.00 32.00 32.00

L-Threonine 48.00 48.00 48.00

L-Tryptophan 10.00 10.00 10.00

L-Tyrosine 36.00 36.00 36.00

L-Valine 46.00 46.00 46.00

Vitamins D-Calcium pantothenate 1.00 1.00 1.00

Choline bitartrate 1.80

Choline chloride 1.00 1.00

Folic acid 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00

Nicotinamide 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 1.00

Riboflavin 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 1.00

Weight (g / L) 10.31 10.61 10.48

NCTC 135 - MediaComponents A2020 A1528

Inorganic salts Calcium chloride x 2H2O 264.92 264.92

Magnesium sulfate dried 139.52 139.52

Potassium chloride 400.00 400.00

Sodium acetate x 3H2O 50.00 50.00

Sodium chloride 6800.00 6800.00

Sodium dihydrogen phosphate x H2O 140.00 140.00

Other Components D(+)-Glucose anhydrous 1000.00 1000.00

L-a-Aminobutyric acid 5.51 5.51

Ethanol 40.00 40.00

Glucosamine x HCl 3.85 3.85

D-(-)-Glucuronic acid sodium salt H2O 1.95 1.95

D-(-)-Glucuronic acid-g-lactone 1.80 1.80

Glutathione (ox.) 10.00 10.00

5-Methylcytosine 0.10 0.10

Phenol red 15.00 15.00

L-Taurine 4.18 4.18

Tween 80 12.50 12.50

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NCTC 135 - MediaComponents A2020 A1528

Amino acids L-Alanine 31.48 31.48

L-Arginine x HCl 31.16 31.16

L-Asparagine x H2O 9.11 9.11

L-Aspartic acid 9.91 9.91

L-Cystine 10.49 10.49

L-Glutamic acid anhydrous 8.26 8.26

L-Glutamine 0 135.73

Glycine 13.51 13.51

L-Histidine x HCl x H2O 24.36 24.36

L-Hydroxyproline 4.09 4.09

L-Isoleucine 18.04 18.04

L-Leucine 20.44 20.44

L-Lysine x HCl 38.43 38.43

L-Methionine 4.44 4.44

L-Ornithine x HCl 9.41 9.41

L-Phenylalanine 16.53 16.53

L-Proline 6.13 6.13

L-Serine 10.75 10.75

L-Threonine 18.93 18.93

L-Tryptophan 17.50 17.50

L-Tyrosine 16.44 16.44

L-Valine 25.00 25.00

Vitamins p-Aminobenzoic acid 0.125 0.125

L-Ascorbic acid 50.00 50.00

D(+)-Biotin 0.025 0.025

Calciferol 0.25 0.25

D-Calcium pantothenate 0.025 0.025

Choline chloride 1.25 1.25

Folic acid 0.025 0.025

myo-Inositol 0.125 0.125

Menadione 0.025 0.025

Nicotinamide 0.0625 0.0625

Nicotinic acid 0.0625 0.0625

Pyridoxal x HCl 0.0625 0.0625

Pyridoxine x HCl 0.0625 0.0625

Riboflavin 0.025 0.025

Thiamine x HCl 0.025 0.025

DL-a-Tocopherolphosphate x Na2 0.025 0.025

Vitamin A acetate 0.25 0.25

Vitamin B12 10.00 10.00

Deoxyribonucleosides 2'-Deoxyadenosine x H2O 10.00 10.00

2'-Deoxycytidine x HCl 10.00 10.00

2'-Deoxyguanosine 10.00 10.00

2'-Deoxythymidine 10.00 10.00

Coenzymes Cocarboxylase x HCl 1.00 1.00

Coenzyme A 2.50 2.50

FAD 1.00 1.00

NAD 7.00 7.00

NADP 1.00 1.00

UTP 1.00 1.00

Weight (g / L) 9.35 9.49

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Page 45: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

PBS - Buffer (after Dulbecco)Components A0964 A0965 A1908

Inorganic salts Potassium chloride 200.00 2000.00 200.00

Potassium dihydrogenphosphate

200.00 2000.00 200.00

Sodium chloride 8000.00 80000.00 8000.00

di-Sodium hydrogen phosphateanhydrous

1150.00 11500.00 1150.00

OtherComponents

EDTA (0,02 %) 1.91

Thimerosal (0,01 %) 0.95

Weight (g / L) 9.55 95.50 9.55

RPMI 1640 MediaComponents A1959 A1339 A1960 A1538 A2040 A3135

Inorganic salts Calcium chloride x2H2O

62.27 62.27 62.27 62.27 62.27 62.27

Magnesiumsulfate dried

69.77 69.77 69.77 69.77 69.77 69.77

Potassiumchloride

400.00 400.00 400.00 400.00 400.00 400.00

Sodium chloride 5950.49 5950.49 5450.49 5450.49 5950.49 5950.49

di-Sodiumhydrogenphosphate

800.00 800.00 800.00 800.00 800.00 800.00

Sodium nitrate 72.00 72.00 72.00 72.00 72.00 72.00

OtherComponents

D(+)-Glucoseanhydrous

2000.00 2000.00 2000.00 2000.00 2000.00 2000.00

HEPES 5958.00 5958.00

Glutathione (red.) 1.00 1.00 1.00 1.00 1.00 1.00

Phenol red 5.00 5.00 5.00 5.00

Amino acids L-Arginine x HCl 241.86 241.86 241.86 241.86 241.86 241.86

L-Asparagine xH2O

50.00 50.00 50.00 50.00 50.00 50.00

L-Aspartic acid 20.00 20.00 20.00 20.00 20.00 20.00

L-Cystine 50.00 50.00 50.00 50.00 50.00 50.00

L-Glutamic acidanhydrous

20.00 20.00 20.00 20.00 20.00 20.00

L-Glutamine 0 300.00 0 300.00 0 300.00

Glycine 10.00 10.00 10.00 10.00 10.00 10.00

L-Histidine Base 15.00 15.00 15.00 15.00 15.00 15.00

L-Hydroxyproline 20.00 20.00 20.00 20.00 20.00 20.00

L-Isoleucine 50.00 50.00 50.00 50.00 50.00 50.00

L-Leucine 50.00 50.00 50.00 50.00 50.00 50.00

L-Lysine x HCl 40.00 40.00 40.00 40.00 40.00 40.00

L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00

L-Phenylalanine 15.00 15.00 15.00 15.00 15.00 15.00

L-Proline 20.00 20.00 20.00 20.00 20.00 20.00

L-Serine 30.00 30.00 30.00 30.00 30.00 30.00

L-Threonine 20.00 20.00 20.00 20.00 20.00 20.00

L-Tryptophan 5.00 5.00 5.00 5.00 5.00 5.00

L-Tyrosine 20.00 20.00 20.00 20.00 20.00 20.00

L-Valine 20.00 20.00 20.00 20.00 20.00 20.00

Vitamins p-Aminobenzoicacid

1.00 1.00 1.00 1.00 1.00 1.00

D(+)-Biotin 0.20 0.20 0.20 0.20 0.20 0.20

D-Calciumpantothenate

0.25 0.25 0.25 0.25 0.25 0.25

Choline chloride 3.00 3.00 3.00 3.00 3.00 3.00

Folic acid 1.00 1.00 1.00 1.00 1.00 1.00

myo-Inositol 35.00 35.00 35.00 35.00 35.00 35.00

Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00

Pyridoxine x HCl 1.00 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.20 0.20 0.20 0.20 0.20 0.20

Thiamine x HCl 1.00 1.00 1.00 1.00 1.00 1.00

Vitamin B12 0.005 0.005 0.005 0.005 0.005 0.005

Weight (g / L) 10.12 10.42 15.57 15.87 10.11 10.41

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Page 46: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Click-RPMI - MediaComponents A2044 A2504

Inorganic salts Calcium chloride x 2H2O 114.39 114.39

Magnesium sulfate dried 104.74 104.74

Potassium chloride 400.00 400.00

Potassium dihydrogen phosphate 30.00 30.00

Sodium chloride 6982.77 6982.77

di-Sodium hydrogen phosphate 425.00 425.00

Sodium nitrate 25.06 25.06

Other Components D(+)-Glucose anhydrous 1500.00 1500.00

Glutathione (red.) 0.50 0.50

Phenol red 7.50 7.50

Amino acids L-Alanine 17.80 17.80

L-Arginine 157.50 157.50

L-Arginine x HCl 100.00 100.00

L-Asparagine x H2O 51.40 51.40

L-Aspartic acid 36.60 36.60

L-Cystine 55.00 55.00

L-Glutamic acid anhydrous 39.40 39.40

L-Glutamine 0 442.00

Glycine 20.00 20.00

L-Histidine Base 7.50 7.50

L-Histidine x HCl x H2O 55.30 55.30

L-Hydroxyproline 10.00 10.00

L-Isoleucine 90.00 90.00

L-Leucine 90.00 90.00

L-Lysine x HCl 111.30 111.30

L-Methionine 26.30 26.30

L-Phenylalanine 47.50 47.50

L-Proline 33.00 33.00

L-Serine 36.00 36.00

L-Threonine 70.00 70.00

L-Tryptophan 15.00 15.00

L-Tyrosine 55.00 55.00

L-Valine 67.50 67.50

Vitamins p-Aminobenzoic acid 0.50 0.50

D(+)-Biotin 0.10 0.10

D-Calcium pantothenate 1.12 1.12

Choline chloride 2.50 2.50

Folic acid 1.50 1.50

myo-Inositol 19.50 19.50

Nicotinamide 1.50 1.50

Pyridoxal x HCl 1.00 1.00

Pyridoxine x HCl 0.50 0.50

Riboflavin 0.20 0.20

Thiamine x HCl 1.50 1.50

Ribonucleosides Adenosine 12.50 12.50

Cytosine 12.50 12.50

Guanosine 12.50 12.50

Uridine 12.50 12.50

Weight (g / L) 10.86 11.30

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Page 47: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Schneider's Drosophila - MediaComponents A2036 A1533

Inorganic salts Magnesium sulfate dried 2585.71 2585.71

Potassium chloride 1600.00 1600.00

Potassium dihydrogen phosphate 450.00 450.00

Sodium chloride 2100.00 2100.00

di-Sodium hydrogen phosphate 700.00 700.00

Other Components D(+)-Glucose anhydrous 2000.00 2000.00

Fumaric acid 60.00 60.00

a-Ketoglutaric acid sodium salt 402.66 402.66

DL-Malic acid 600.00 600.00

Succinic acid 60.00 60.00

Trehalose x 2H2O 2210.00 2210.00

Yeast extract 2000.00 2000.00

Amino acids b-Alanine 500.00 500.00

L-Arginine Base 600.00 600.00

L-Aspartic acid 400.00 400.00

L-Cysteine 60.00 60.00

L-Cystine 16.60 16.60

L-Glutamic acid anhydrous 800.00 800.00

L-Glutamine 0 1800.00

Glycine 250.00 250.00

L-Histidine Base 400.00 400.00

L-Isoleucine 150.00 150.00

L-Leucine 150.00 150.00

L-Lysine x HCl 1650.00 1650.00

L-Methionine 150.00 150.00

L-Proline 1700.00 1700.00

L-Serine 250.00 250.00

L-Threonine 350.00 350.00

L-Tryptophan 100.00 100.00

L-Tyrosine 500.00 500.00

L-Valine 300.00 300.00

Weight (g / L) 23.09 24.84

Comment Before dissolving the Schneider'sDrosophila - Medium

you have to add 794,76 mg/L CaCl2 2H2Oto the water!

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Page 48: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

TC-100 - MediaComponents A2033 A2017

Inorganic salts Calcium chloride x 2H2O 1298.11 1298.11

Magnesium chloride x 6H2O 2282.59 2282.59

Magnesium sulfate dried 1957.14 1957.14

Potassium chloride 2900.00 2900.00

Sodium dihydrogen phosphate x H2O 970.00 970.00

Other Components D(+)-Glucose anhydrous 1000.00 1000.00

Bacto - Tryptose 2600.00 2600.00

Amino acids L-Alanine 225.00 225.00

L-Arginine Base 550.00 550.00

L-Asparagine x H2O 391.97 391.97

L-Aspartic acid 350.00 350.00

L-Cystine 20.00 20.00

L-Glutamic acid anhydrous 600.00 600.00

L-Glutamine 0 600.00

Glycine 650.00 650.00

L-Histidine x HCl x H2O 3400.00 3400.00

L-Isoleucine 50.00 50.00

L-Leucine 75.00 75.00

L-Lysine x HCl 630.00 630.00

L-Methionine 50.00 50.00

L-Phenylalanine 150.00 150.00

L-Proline 350.00 350.00

L-Serine 550.00 550.00

L-Threonine 180.00 180.00

L-Tryptophan 100.00 100.00

L-Tyrosine 55.00 55.00

L-Valine 100.00 100.00

Vitamins p-Aminobenzoic acid 0.02 0.02

D(+)-Biotin 0.01 0.01

D-Calcium pantothenate 0.11 0.11

Folic acid 0.02 0.02

myo-Inositol 0.02 0.02

Nicotinic acid 0.02 0.02

Pyridoxine x HCl 0.02 0.02

Riboflavin 0.02 0.02

Thiamine x HCl 0.02 0.02

Vitamin B12 0.01 0.01

Weight (g / L) 21.49 22.09

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Page 49: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

TNM-FH - MediaComponents A2037 A1570

Inorganic salts Calcium chloride x 2H2O 1324.60 1324.60

Magnesium chloride x 6H2O 2278.86 2278.86

Magnesium sulfate dried 1939.80 1939.80

Potassium chloride 2240.00 2240.00

di-Sodium hydrogen phosphate 876.92 876.92

Other Components D(+)-Glucose anhydrous 700.00 700.00

D(-)-Fructose 400.00 400.00

Fumaric acid 55.00 55.00

a-Ketoglutaric acid sodium salt 425.66 425.66

Lactalbumin Hydrolysate 3333.33 3333.33

DL-Malic acid 670.00 670.00

Succinic acid 60.00 60.00

D(+)-Sucrose 26680.00 26680.00

Yeast extract 3333.33 3333.33

Amino acids L-Alanine 200.00 200.00

L-Arginine x HCl 700.00 700.00

L-Asparagine x H2O 350.00 350.00

L-Aspartic acid 350.00 350.00

L-Cystine 19.18 19.18

L-Glutamic acid anhydrous 600.00 600.00

L-Glutamine 0 600.00

Glycine 650.00 650.00

L-Histidine Base 2500.00 2500.00

L-Isoleucine 50.00 50.00

L-Leucine 75.00 75.00

L-Lysine x HCl 625.00 625.00

L-Methionine 50.00 50.00

L-Phenylalanine 150.00 150.00

L-Proline 350.00 350.00

L-Serine 550.00 550.00

L-Threonine 175.00 175.00

L-Tryptophan 100.00 100.00

L-Tyrosine 50.00 50.00

L-Valine 100.00 100.00

Vitamins p-Aminobenzoic acid 0.02 0.02

D(+)-Biotin 0.01 0.01

D-Calcium pantothenate 0.02 0.02

Choline chloride 0.20 0.20

Folic acid 0.02 0.02

myo-Inositol 0.02 0.02

Nicotinic acid 0.02 0.02

Pyridoxine x HCl 0.02 0.02

Riboflavin 0.02 0.02

Thiamine x HCl 0.02 0.02

Weight (g / L) 52.19 52.79

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Page 50: Cell Culture Powder Media B - AppliChem · Cell Culture Powder Media B HS-No.: 38220000 Storage: 2-8˚C LGK: 10 - 13 ... A1312,9010 10 L A1312,9050 50 L BME, Basal medium with Earle's

Waymouth's MB 752/1 - MediaComponents A1973 A1527 A1981 A1961

Inorganic salts Calcium chloride x 2H2O 120.02 120.02 120.02 120.02

Magnesium chloride x 6H2O 240.00 240.00 240.00 240.00

Magnesium sulfate dried 130.96 130.96 130.96 130.96

Potassium chloride 150.00 150.00 150.00 150.00

Potassium dihydrogenphosphate

80.00 80.00 80.00 80.00

Sodium chloride 6000.00 6000.00 5500.00 5500.00

di-Sodium hydrogen phosphateanhydrous

300.00 300.00 300.00 300.00

OtherComponents

D(+)-Glucose anhydrous 5000.00 5000.00 5000.00 5000.00

HEPES 4766.40 4766.40

Glutathione (red.) 15.00 15.00 15.00 15.00

Hypoxanthine 25.00 25.00 25.00 25.00

Phenol red 10.00 10.00 10.00 10.00

Amino acids L-Arginine x HCl 75.00 75.00 75.00 75.00

L-Aspartic acid 60.00 60.00 60.00 60.00

L-Cysteine x HCl x H2O 100.26 100.26 100.26 100.26

L-Cystine 15.00 15.00 15.00 15.00

L-Glutamic acid anhydrous 150.00 150.00 150.00 150.00

L-Glutamine 0 350.00 0 350.00

L-Glycine 50.00 50.00 50.00 50.00

L-Histidine x HCl x H2O 164.10 164.10 164.10 164.10

L-Isoleucine 25.00 25.00 25.00 25.00

L-Leucine 50.00 50.00 50.00 50.00

L-Lysine x HCl 240.00 240.00 240.00 240.00

L-Methionine 50.00 50.00 50.00 50.00

L-Phenylalanine 50.00 50.00 50.00 50.00

L-Proline 50.00 50.00 50.00 50.00

L-Threonine 75.00 75.00 75.00 75.00

L-Tryptophan 40.00 40.00 40.00 40.00

L-Tyrosine 40.00 40.00 40.00 40.00

L-Valine 65.00 65.00 65.00 65.00

Vitamins L-Ascorbic acid 17.50 17.50 17.50 17.50

D(+)-Biotin 0.02 0.02 0.02 0.02

D-Calcium pantothenate 1.00 1.00 1.00 1.00

Choline chloride 250.00 250.00 250.00 250.00

Folic acid 0.40 0.40 0.40 0.40

myo-Inositol 1.00 1.00 1.00 1.00

Nicotinamide 1.00 1.00 1.00 1.00

Pyridoxine x HCl 1.00 1.00 1.00 1.00

Riboflavin 1.00 1.00 1.00 1.00

Thiamine x HCl 10.00 10.00 10.00 10.00

Vitamin B12 0.20 0.20 0.20 0.20

Weight (g / L) 13.65 14.00 17.92 18.27

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William's Media EComponents A1309 A1910 A1966 A1962 A3029

Inorganic salts Calcium chloride x 2H2O 264.92 264.92 264.92 264.92 264.92

Copper(II)-sulfate x 5H2O 0.0001 0.0001 0.0001 0.0001 0.0001

Iron(III)-nitrate x 9H2O 0.0001 0.0001 0.0001 0.0001 0.0001

Magnesium sulfate dried 139.57 139.57 139.57 139.57 127.01

Manganese chloride x 4H2O 0.0001 0.0001 0.0001 0.0001 0.0001

Potassium chloride 400.00 400.00 400.00 400.00 400.00

Sodium chloride 6800.00 6800.00 6300.00 6300.00 6800.00

Sodium dihydrogen phosphate xH2O

140.00 140.00 140.00 140.00 140.00

Zinc sulfate x 7H2O 0.0002 0.0002 0.0002 0.0002 0.0002

OtherComponents

D(+)-Glucose anhydrous 2000.00 2000.00 2000.00 2000.00 2000.00

HEPES 5958.00 5958.00

Glutathione (red.) 0.05 0.05 0.05 0.05 0.05

Methyllinoleate 0.03 0.03 0.03 0.03 0.03

Phenol red 10.00 10.00 10.00 10.00

Sodium pyruvate 25.00 25.00 25.00 25.00 25.00

Amino acids L-Alanine 90.00 90.00 90.00 90.00 90.00

L-Arginine Base 50.00 50.00 50.00 50.00 50.00

L-Asparagine x H2O 20.00 20.00 20.00 20.00 20.00

L-Aspartic acid 30.00 30.00 30.00 30.00 30.00

L-Cysteine 40.00 40.00 40.00 40.00 40.00

L-Cystine 20.00 20.00 20.00 20.00 20.00

L-Glutamic acid anhydrous 50.00 50.00 50.00 50.00 50.00

L-Glutamine 0 292.00 0 292.00 0

Glycine 50.00 50.00 50.00 50.00 50.00

L-Histidine Base 15.00 15.00 15.00 15.00 15.00

L-Isoleucine 50.00 50.00 50.00 50.00 50.00

L-Leucine 75.00 75.00 75.00 75.00 75.00

L-Lysine x HCl 87.50 87.50 87.50 87.50 87.50

L-Methionine 15.00 15.00 15.00 15.00 15.00

L-Phenylalanine 25.00 25.00 25.00 25.00 25.00

L-Proline 30.00 30.00 30.00 30.00 30.00

L-Serine 10.00 10.00 10.00 10.00 10.00

L-Threonine 40.00 40.00 40.00 40.00 40.00

L-Tryptophan 10.00 10.00 10.00 10.00 10.00

L-Tyrosine 35.00 35.00 35.00 35.00 35.00

L-Valine 50.00 50.00 50.00 50.00 50.00

Vitamins L-Ascorbic acid 2.00 2.00 2.00 2.00 2.00

D(+)-Biotin 0.50 0.50 0.50 0.50 0.50

Calciferol 0.10 0.10 0.10 0.10 0.10

D-Calcium pantothenate 1.00 1.00 1.00 1.00 1.00

Choline chloride 1.50 1.50 1.50 1.50 1.50

Folic acid 1.00 1.00 1.00 1.00 1.00

myo-Inositol 2.00 2.00 2.00 2.00 2.00

Menadione sodium bisulfite 0.01 0.01 0.01 0.01 0.01

Nicotinamide 1.00 1.00 1.00 1.00 1.00

Pyridoxal x HCl 1.00 1.00 1.00 1.00 1.00

Riboflavin 0.10 0.10 0.10 0.10 0.10

Thiamine x HCl 1.00 1.00 1.00 1.00 1.00

DL-a-Tocopherolphosphate-Na2 0.01 0.01 0.01 0.01 0.01

Vitamin A acetate 0.10 0.10 0.10 0.10 0.10

Vitamin B12 0.20 0.20 0.20 0.20 0.20

Weight (g / L) 10.58 10.88 16.04 16.33 10.57

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