Cell Bio Midterm Hitlist
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Transcript of Cell Bio Midterm Hitlist
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EM Cell Type Lab Identify:
EM1 pancreas III rough ER
zymogen granules
euchromatin
heterochromatin
nucleus and nucleolus
mitochondria
acinus and lumenintercellular space
extracellular space
golgi
EM2 liver (3 hepatocytes) III mitochondria
golgi
RER
glycogen
autophagosomes/autophagolysomes
peroxisomes
outer mitochondrial membraneinner mito memb and cristae
mitochondrial matrix
VII Gap Junction
intermembrane space
IV lipid droplets
EM 2a Freeze fracture of gap jun VII Gap Junction
EM2b liver, cytoskeletal element IV microtubule
microfilament
intermediate filament
V heterochromatinnucleolus
EM3 intestinal absorptive cell ( II basement membrane (collagen)
basal lamina
mitochondria
interdigitations of plasma membranes of a
EM4 intestinal absorptive and II microvilli and brush border
(apical end) glycocalyx
absorptive cell
goblet cell (mucus droplets)
IV terminal web
chylomicrons
secretory granules
microfilaments
VII Zonula Adherens
Zonula Occludens
Terminal web
EM 4a Freeze fracture of tight ju VII tight junction
EM5 Nucleus (liver) w/nuclear V Nulceus
Nuclear Pores
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heterochromatin
outer membrane of nuclear envelope
inner membrane of nuclear envelope
periplasmic space
ribosomes on outer membrane
EM 6 Nucleus-freeze fracture V Outer nuclear membrane
inner nuclear membrane
nuclear poresEM6a insect spermatocyte IV centrioles
centrioles and microtubules microtubules
EM6b monkey oviduct IV axoneme of cilia (doublet and singlet MTs)
cilia and basal bodies in crosssecti basal bodies
VII Desmosome
Zonula Adherens
Zonula Occludens
EM6c monkey oviduct IV doublets in cilia continous with basal bodie
cilia and basal bodies in longitudinal
EM7 Ascites fluid cell III golgi and vesciclesmitochondria
nucleus
Lysosomes
RER
EM 8a Bone Marrow VI Erythrocyte
Reticulocyte
Eosinophil precursor
Neutrophil
XIII polychromatophilic/orthochromatic erythro
nurse celleosinophilic precursor (myelocyte)
EM 8c Basophil VI basophil
EM8d Mast Cell VIII Mast Cell Granule
EM 8e Fibroblast and Collagen VIII Fibroblast cell
Collagen Fiber
EM 8f Peripheral Blood VI Lymphocytes
Neutrophils
Monocytes
Azurophilic granules
Neutrophil specific granules
Eosinophil
EM 9 Smooth muscle IX Elastin
Endothelium
Lumen
Calveolae Intracellulares (Vesicles)
Myofilaments
Mitochondria
Lysosomes
Rough ER
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Dense Bodies
XI Lumen
Smooth Muscle Nucleus
Elastin
Endothelial Cell Nucleus
Endothelial Cell Junction
EM 10 skeletal muscle IX Triads
H-BandsI-Bands
A-Bands
Z-Line
Mitochondria
Calsequestrin
T-Tubule
Terminal Cisternae
Sarcomere
EM 10a capillary XI Capillary
Junctional Complex (containing Fascia adheVII Lumen
Endothelial cell cytoplasm
Cell-cell junction
Basal lamina
Vesicles
EM10b cardiac muscle III mitochondria
IX Cardiac Myocytes
Fascia Adherens
Desmosomes
SarcomereA-Band
I-Band
H-Band
Z-Line
Diad
T-Tubule
Terminal Cisternae
Gap Junction
EM 10c nerve cell body X Auerbach Plexus
Neuronal Cell Cytoplasm
Neuronal Cell nucleus
Axon Terminals
Synapse
Enteric Glial Cell Cytoplasm
EM10d Schwann cell III golgi
IV lipid droplets
VIII Collagen Type 1
Collagen Type IV
X Schwan Cells
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Myelinating Schwann Cell
Non-Myelinating Schwann Cell
myelinated axon
unmyelinated axon
Myelin
Epineurium
Perineurium
EndoneuriumEM 10e Smooth muscle IX Dense Bodies
Calveolae Intracellulares (Vesicles)
Collagen Fibers
Nerve Ending
External Lamina
Myofilaments
Mitochondria
X Smooth Muscle Neuromuscular Junction
Smooth Muscle
Presynaptic TerminalMitochondria
Vesicles
Synaptic Cleft
EM11 perichondrium XII chondroblasts
perichondrial cell
chondrocyte
type I collagen
type II collagen
EM12 osteoblasts XII osteoblasts
rough ERgolgi
collagen fibrils
hydroxyapatite crystals
cellular projections in canaliculi
EM12a bone marrow XIII nurse cell
erythrocyte progenitors
EM13 chondrocytes XII newly synthesized matrix
rough ER
mitochondria, nucleus, golgi
EM15 Parietal cell (stomach) VIII nucleolus
EM17 duodenum XI Pericyte
Endothelial Cell
Endothelial cytoplasm
Endothelial Nucleus
Basal Lamina
Pericyte Process
EM20 Leydig cell III mitochondria
IV lipofuscin granules
EM24 corpus luteum IV lipid droplets
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EM25 ovary IV crystalloid inclusions
EM27 rat intestines III golgi and vescicles
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What to look for:
highly osmophilic; condensing granules are lighter than mature granules
big gap
dark staining granules slightly larger than ribosomes (rosette-like aggregates)
osmiophilic crystalloid core
region where membranes come together but do not fuse
area where lots of holes are congregated.
outer cytoplasm, underneath plasma membrane; criss-crossing threads
osmiophilic, lines nuclear laminadense core of the nucleus
fuzzy white stuff
fuzzy gray line immediately subjacent to plasma membrane on basal end
jacent cells
composed of microfilaments
top fuzzy gray coat
composed of microfilaments; organelles excluded from this region
membrane-bound clusters
homogeneously staining inclusions
compose microvilli
cytoplasmic area beneath plasma membrane. No organelles hang out here
honeycomb patterned fine lines
where the periplasmic space disappears
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space between the inner and outer nuclear envelopes
little dots decorating the outer nuclear membrane
the bottom of the crater
space under the hole of the outer nuclear membrane
look live rivet holes on the outer nuclear membrane
nine sets of triplet MTs
Darker staining defined area of intercellular plasma membrane come together, about the
area above desmosome, looks like there is space between the intercelluar membranes
darker line in plasma membrane junctions, just apical to zonula occludens
s
the most osmiophilic solid, amorphous blob, no organelles
the 2nd most osmiophilic solid, amorphous blob, no organelles
large, regular granules that are osmiophilic
next to the developing RBCs
lasts
electron-dense granules containing debris from discarded nuclear contents of differentiatiextensive rER and specific granules
only thing in the EM. Bilobed nucleus, chock-full of dark homogenous granules
the only thing in the cytoplasm
cross section. A grouping of dots extrecellularly
no granules, large amourphous nucleus
Multi-lobed nuclei, granules of varying osmophilicity
no granuels, horseshoe or lobed nucleus that is much smaller than lymphocites
the largest granules
contain what looks like highly osmiophilic equal signs, '='
Granules are large, dark and homogenous
Located between Smooth muscle cell and endot Located in between Sarcomeres, Use Cal
Surrounds lumen Located in Center of Sarcomere Just outsi
Light bands Anchored to Z-Line
Dark Bands containing H bands
Located within Smooth Muscle Cell Dark Line Perpendicular to I bands
Identify by Cristae round organelles located between muscl
Dark spots used to identify Triad
Identify by Ribosomes Located inbetween Calsequestrin protein
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Appear as thickened bands in cell membrane ligher round tubules continuous with SR
Upper Right hand Corner Z-line to Z-line
Identify by recognizing smooth muscle fibers surrounding nucleus and pill shaped nucleus
Located between smooth muscle and endothelial cell
irregular shape nucleus outlined in heterochrom Dense staining filament with light stainin
dense line located between 2 endothelial cells Dense staining filament with light stainin
predominant due to secretory activityLight staining band near Z-Line
note tubular cristae (tubulovesicular in tr
Dark band Perpendicular to thin an thick filamenirregularly shaped, dense deposits
dark staining part
light staining part
Dense bands between adjacent endothelial cells
where membranes of the two endothelial cells meet
the fine dark line that borders the outside of the endothelial cells
Located within Endothelial Cells
\
Rough ER, free polysomes and multiple mitochondria
Bottom of EM, Surrounded by heterochromatin,
Contain multiple synaptic vesicles
Dark line near axon terminal
contain densely packed intermediate filaments
Series of dots in cross section, longitudinall a fine line floating in the ECM
along the extermal lamina of the schwann cell
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Large Nucleus next to myelinated axon
cytoplasm surrounds axon terminals at top of EM
Axon surrounded by dark gray ring
axons located top of EM
Dark Gray ring around axon
bottom left corner of EM
contains nucleus at bottom of EM
Connective tissue visible between Schwan Cell and Nucleus of Perineurium
Continuous Border surrounding
Dark Gray longitudinal fibers Nucleus bulges into Lumen
Lightly stained ring, filled with vesicles, contains surround Capillary Lumenlocated in presynaptic terminal 2
located in presynaptic terminal
space between smooth muscle and presynaptic terminal
differentiate phagolysosomes and mitochondria
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size of a basal body
ng erythrocytes
equestrin to Identify
de M-Line
fibers
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strip down the center
strip down the center
nsverse)
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Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L
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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus does not bul
Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border su
Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into L
Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary Lu
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e into Lumen
rounding
men
en
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term Exam: Slide Hitlist
Slide Tissue Type: Lab
102 skin V
104 thumb skin XIV
105 Finger skin VII
XIV
107 idermal sheet, DOPA reacti XIV
108 Scalp Skin VIII
XIV
109 skin (monkey) X
XI
V
110 peripheral blood smear I & VI
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111 Forearm Skin VII
XIV
112 bone marrow aspirate XIII
May-Grunwald-Giemsa stain
113 peripheral blood smear VI
121 bone (rabbit femur) XII
XIII
122 Spleen VIII
125 heart IX
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126 bile duct with portal vein XI
(monkey)
128 thoracic duct XI
130 submandibular gland X
VII
133 trachea (monkey) X
XI
VII
VIII
136 tongue (monkey) IX
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XI
137 esophagus III
IX
140 Infected Cardiac Stomach VI
146 duodenum (monkey) IX
VIII
147 pyloroduodenal junction IX(baboon)
148 ileum II
V
VI
VIII
X
149 mall intestine (mouse, PAS II
VII
152 duodenum X
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155 ll bladder and liver (monke XI
V
156 creas (monkey, toluidine bl III
157 pancreas (monkey) I
V
158 pancreas III
X
160 urinary bladder (monkey) IX
VII179 fallopian tube IV
VII
182 Lactating Mammary Gland VII
186 adrenal XI
193 peripheral nerve (monkey) X
X
194 fetal elbow XII
195 fetal jaw XII
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196 Vessels and Endothelium VII
199 epididymis XI
202 Tendon VIII
205 idermal sheet, DOPA reacti XIV
206 abdominal skin VIII
XIV
207 skin, abdominal (Gallego's) XIV
208 newborn skin XIV
208 skin (newborn) III
VIIIXI
209 scalp XIV
210 skeletal muscle IX
Masson's trichrome stain
X
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XI
211 skeletal muscle IX
IX
220 fetal finger I
XII
225 heart (epicardium) IX
226 heart (endocardium) IX
227 lastic and muscular arterie XI
elastin stain
231 Blood Smear, Leukemia VI
XIII
401 icine and Cytochalasin Cult V
405 skeletal muscle and tumor IV
desmin stain
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IX
406 ooth muscle (intestine, PA IX
407 skeletal muscle and tendon IX(guinea pig)
408 sole of foot (skin) XIV
420 rib XI
XII
XIII
421 tibia XII
422 bone (femur, monkey) XII
XIII
425 bone (unstained) XII
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426 al artery and vein with ner X
XI
427 aorta XI
432 Lung VIII
439 salivary gland IX(stained for smooth muscle actin)
447 denum (monkey, toluidine b II
VIII
XI
472 fallopian tube IV
480 Vagina VII
481 reast Tumor (toludine blue VIII
492 eripheral ganglion (monkey X
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Identify:
Interphase Cell
Prophase Cell
Metaphase Cell
Anaphase Cell
Telophase CellCell in Cytokinesis
epidermis
stratum corneum
Meissner's corpuscles
single, unbranched duct
epidermis
dermis
subdermis
duct of sweat gland
secretory portion of sweat glandPacinian corpuscles
melanocyte
epidermis
dermis
collagen
subdermis
fibrolasts
white adipose tissue
melanocyte
Langerhans cellhair follicle
external root sheath
internal rooth sheath
sebaceous gland
dermal papillae
differentiation zone
arrector pili muscle
Pacinian Corpuscles
Small Artery
Small Veins
Arteriole
Venule
Capillary
Viable Keratinacyte
RBC's
Neutrophil
Lymphocyte
Monocyte
Platelet
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Intercalated Discs
accumulated lipofuscin granules
Cystic Duct
Bile Duct
Portal vein
Hepatic Artery
Layers of Hepatic Atery
Small ArterySmall Vein
Arteriole
Arteriole (longitudinal)
Venule
Capillary
Lymphatic Vessels
Thoracic Duct
Lumen
Intima
MediaAdventitia
Endoneurium
myelinated axons
unmyelinated axons
Capillaries
Perineurium
Epineurium
serous gland cells
mucin secreting cells
ductEndoneurium
myelinated axons
unmyelinated axons
Capillaries
Perineurium
Epineurium
Small Artery
Small Vein
Arteriole
Venule
Capillary
pseudostratified epithelium
brown adipose tissue
Gland
Capillaries
A Band
I Band
Z Line
Epithelium
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Fat
Fascicle Cross section
Fascicle longitudinal
Perimysium
Capillary
folds and wrinkles
Skeletal Muscle Transverse
Skeletal Muscle LongitudinalSmooth Muscle Transverse
Smooth Muscle Longitudinal
WBCs
Collagenous Connective Tissue
Smooth Muscle longitudinal
Smooth Muscle Transverse
Mast Cell
Macrophage
plasma cell
Smooth Muscle TransverseSmooth Muscle Longitudinal
Collagenous Connective Tissue
epithelial cells lining villi and their nuclei
goblet cells lining villi
long thin nuclei in absorptive cells
apical vs basal end
brush border
microvilli
Paneth Cells
NeutrophilEosinophil
connective tissue
Mast Cell
Macrophage
Auerbach Plexus
Neuronal Cell Bodies
Enteric Glial Cells
Meissner's Plexus
Neuronal Cell Bodies
Enteric Glial Cells
carbohydrates
simple columnar epithelium
Auerbach Plexus
Neuronal Cell Bodies
Enteric Glial Cells
Meissner's Plexus
Neuronal Cell Bodies
transverse sections of villi: epithelial cells (absorptiveand goblet cells), basement membrane
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Enteric Glial Cells
Sinusoids
Binucleated cells
acinar cells
acinus, acinar cell, and nucleus (100x) with heterochr
basophilic nucleus
basophilic basal (lower) cytoplasm (RNA)
acidophilic apical cytoplasm (secretory granules)heterochromatin
pancreatic acinar cell and nucleus
acidophilic granules in apical cytoplasm
Peripheral Nerve tissue
Collagen
Smooth Muscle
Collagenous Connective Tissue
Smooth Muscle longitudinal
Smooth Muscle Transverse
transitional epitheliummotile cilia
basal bodies
simple columnar epithelium
basal bodies
alveoli
Sinusoids
Longitudinal Section
Endoneurium
Perineurium
EpineuriumMast Cell
Schwann Cell Nuclei
Node of Ranvier
Schmidt-Lanternman cleft
Transverse Section
Endoneurium
myelinated axons
unmyelinated axons
Capillaries
Perineurium
Epineurium
hyaline cartilage
perichondrium (contains perichondrial cells)
chondrocytes, condroblasts, lacunae
bone
vascularized mesnechymal tissue
osteoblasts
osteocytes
osteoclasts
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simple squamous epithelium
Lymphatics
Lumen
Intima
Media
Adventitia
Collagen
fibroblastsmelanocyte
Macrophage
epidermis
dermis
subdermis
dermal-epidermal junction
dermal papillae
epidermal pegs
sweat gland duct
capillariescollagen fibers
sweat gland (secretory)
adipose cells, blood vessels and nerves of subdermis
melanin within keratinocytes
Langerhans cells
melanocyte
review skin components
review skin components
empty spaces (formerly fat droplets)
white adipose tissueMuscular Artery
Small Vein
hair follicle
external root sheath
internal rooth sheath
dermal sheath
sebaceous gland
dermal papillae
differentiation zone
arrector pili muscle
Epimysiusm
Perimysium
Fascicles
Muscle Spindle
Intrafusal Fibers
Extrafusal Fibers
Nerve tissue
Perineurium
Collagen of Epimysium
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Capillaries
Muscular Artery
Small Vein
Transverse Section
Skeletal Muscle Fibers
Endomysium
Longitudinal Section
NucleiEndomysium
A Band
I Band
Z Line
Sarcomere
acidophilic areas (proteins)
basophilic areas (sulfates)
hyaline cartilage
perichondrium (contains perichondrial cells)
chondrocytes, chondroblasts, lacunaespongy bone
trabeculae (w/in spongy bone)
bone marrow/hemapoetic tissue
calcified cartilage
osteoclasts
osteoblasts
osteocytes
epiphyseal region
endochondral ossification
EpicardiumMyocardium
Visceral Pericardium
Intercalated Discs
Coronary Vessels
lipofuscin granules
Myocardium
Endocardium
Purkinje Fibers
Smooth Muscle
Endothelial Cells
aorta
Aortic Elastic Lamina
Muscular Artery
WBCs
precursors to eosinophils and basphils (see hit list for
Cells treated with Colchicine
Cells treated with Cytochalasin
positive (brown) stain in skeletal muscle
brown stain in tumor cells
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Z-line
Muscle Fibers
Nuclei
Smooth Muscle Cell Nuclei Longitudinal
External Lamina Longitudinal
Contracted smooth muscle
External Lamina Transverse
Skeletal MuscleTendons
epidermis
stratum corneum
epidermal pegs
Muscular Artery
Small Vein
trabeculae
bone marrow
osteoblasts
osteoclastslacunae and osteocytes
canaliculi
bone marrow
megakaryocytes
adipocytes
spongy bone
calcified cartilage
trabeculae (w/in spongy bone)
hematopoetic tissue
osteocyteszone of proliferation
zone of hypertrophy
zone of calcification
zone of bone deposition and remodeling
osteoblasts
periosteum
outer circumferential lamellae
medullary cavity
adipocytes
bony trabeculae
bone marrow
Sinusoids
cells of extravascular compartment
megakaryocytes
osteon/Haversian system
Haversian canal
concentric lamellae
osteocytes, lacunae
canaliculi
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Look for:
quiescent, intact membrane with multiple nuclei
condensed chromosomes in a haphazard array. Mitotic spindle formation
fully condensed chromosome centered in the middle plane. From single line for "floral" arr
two chromosomal groups separated by microtubule procceses
decondensation of chromosomes, formation of nuclear envelopenuclear envelopes formed but cytoplasm still attached
thickened
prominent, with deep epidermal pegs
in dermal papillae, recognized by pattern of Schwann cells
a continuaion of epidermal cells inward to form a tubular duct in the dermis, running towar
stratified, cuboidal epithelium, 2 layers
pseudostratified epithelium containing clear cells and dark cells (secretory granules)deep dermis and subdermis, concentric lamellar structure
stellate appearance; note hill and valley appearance of epidermis
dark staining, most superficial layer
subjeacent to dermis, dense connective tissue
eosinophilic, in areas that are devoid of cells
loose connective tissue, beneath dermis, lots of adipocytes in the area
in connective tissue, plump cells with oval nuclei with 1 prominent nucleolus or can be elon
where the large holes are, have huge lipid droplet, little cytoplasm and a flattened nucleus
clear cell in basal cell layer
clear, stellate cell in spinous layer; may be able to see processescontinuous with epidermis
group of cells adjacent to follicles
smooth muscle that connect lower half of root sheaths with upper portion of dermis
light eosinophilic stain, concentric, lamellar appearance
just underneath the basophilic, superficial keratinized layer
acidophilic, note size
highly segmented nucleus and pale cytoplasm
large, round nucleus, heavily condensed chromatin, thin rim of light blue cytoplasm
Largest cell in peripheral blood, 3X RBC, large kidney-shaped nucleus
anucleate bodies in clumps, look like specks
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thin magenta line directly under epithelia
darker staining region at the apical end of the boundaries between cells, very subtle in ligh
observe the multi-layered basophilic epidermis with thin outer layer noting the change in s
at 100X note the artifactual spaces between cells that remain attached at points by desmo
attached by hemidesmosomes to the dermis
spines or prickles represent desmosomal attachments
one or two layers of darkly staining keratohyalin granulescompletely keratinized, exfoliating cells
pale cells in basal cell layer that appear unconnected to adjacent cells and often protrude s
avoid densely packed areas; areas around adipocytes are most representative of cell popul
large cells; multilobe nucleus; purple-staining cytoplasm due to abundant granules; should
large phatocytotic cells with long processes and normally attached to reticular fibers; in pre
large cell with large, centrally located nucleus; nuclear chromatin is finely dispersed, giving
nuclear condensation, blue cytoplasm (know distinction from proerythroblast)
more chromatin condensation; variable cytoplasm color (gray or pink-blue); smaller than pr
nucleus is pyknotic mass, ready to be extruded; cytoplasm looks like that of reticulocyteno nucleus; light gray cytoplasm; larger than mature erythrocyte
oval, eccentrically-placed nucleus containing finely dispersed chromatin; blue cytoplasm wi
increasingly pink cytoplasm with blue in later stages
cytoplasm is color of mature neutrophil (no basophilic ER present); nucleus has minimal clu
nucleus has condensed and become horse-shoe shaped
little nuclear condensation and eosinophilic granules
most abundant, eosiniphilic
tri-lobed nucleus and very mildly eosinophilic cytoplasm
bi-lobed nucleus, bright red granuleslarge, round nucleus, heavily condensed chromatin, thin rim of light blue cytoplasm
Largest cell in peripheral blood, 3X RBC, large kidney-shaped nucleus
anucleate bodies in clumps, look like specks
lines haversion canals, bone trabeculae, and compact bone surrounding marrow cavity
first few layers of compact bone next to endosteum
surrounded by concentric circles of lamellae, containing osteocytes
multiple layers of osteoprogenitor cells and connective tissue outside the bone
may see many artifacts where lipid droplets have been extracted
homogenous eosinophilic stain
large, thin-walled vessels lined by endothelial cells and filled with mature blood cells, prima
large cells with multi-lobded nuclei that have easinophilic cytoplasm\
eosiniphilc band running down a side of the tissue sample. Composed of dense connective
Centrally located nuclei with branched fibers, notice fibers consists of multiple myocytes
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slightly brighter stained band between cardiomyocytes
Smallest lumen, lined by columnar epithelia
located between Portal vein and Cystic Duct, lined by columnar epithelia
largest lumen
2nd smallest lumen
From Lumen: Endothelium, Internal Elastic Lamina, Media, Adventitia
Media containing up to 8 layers of smooth muscle, stains brighter, small lumen
Media containing 1-2 layers of smooth muscle
only a few nuclei surrounding lumen
homogeneously stained lymph
homogeneously stained lymph
thin layer of endothelia
spiral smooth muscleconnective tissue and vasa vasorum in adventitia
Eosinophilic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie tis
dark staining rings in endoneurium
pale specks in endoneurium around myelinated axons
Appear as empty space in endoneurium
Thin layer of cells surrounding endoneurium, Notice absense of axons
Mostly translucent space with interspersed thick sheaths of connective tissue surrounding f
contain zymogen granuales and arranged in simple acini
scattered among serous cells, flat nuclei along the basal membrane, mucin vesicles give cy
Eosinophillic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie ti
dark staining rings in endoneurium
pale specks in endoneurium around myelinated axons
Appear as empty space in endoneurium
Thin layer of cells surrounding endoneurium, Notice absense of axons
Mostly translucent space with interspersed thick sheaths of connective tissue surrounding f
Irregularly shaped lumen
Irregularly shaped lumen, thin wall
note how every cell in the tracheal lining is attached to the basememnt membrane not not
adipocytes have a central, round nucleus, and cytoplasm has many small lipid droplets. Dr
bringhtly stained pink
Appear as gaps in muscle fibers, be sure to see endothelial cells to confirm not an artifact
Strongest Staining with Hematoxylin
light staining with hematoxylin
second strongest staining with hematoxylin
brightly stained pink at edge of tissue
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Clear Artifacts
Eosinophilic, multinucleated cannot see strations
Eosinophilic, multinucleated can see striations
thin border aroudn fascicle, easier to see on cross section sample
Use endothelial cell to differentiate between capillary and gap
artifacts
Nuclei are peripherally located, appear more uniformily stained
Nuclei are peripherally located, appear more uniformily stainedNuclei are centrally located, more densly nucleated
Nuclei are centrally located, more densly nucleated
look in vessels, ECM connective tissue. Note the proliferation of WBCs mediating the infla
Nuclei located in the periphery of cells, stain brighter pink
Long and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi
small diameter, due to transverse section nuclei may appear to be missing
look in lamina propria, round, central nucleus with brightly staining granules
voluminous, heterogeneous cytoplasm, large cells with irregular shape in the connective ti
dark staining cytoplasm (basophilic) and cartwheel nucleus, located in lamina propria
small diameter, due to transverse section nuclei may appear to be missingLong and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi
Nuclei located in the periphery of cells, stain brighter pink
be able to identify different images created by sectioning at different angles
darkly staining mucus in cytoplasm; secreting cells will show gray mucus
purple stain
apical end faces the lumen; acidophilic basement membrane is thin pink line beneath epith
thick acidophilic band on apical surface
at the base of crypts, have bright red granules that contain lysozyme
in large vessels and ECM, tri-lobed nucleus and very mildly eosinophilic cytoplasmin large vessels and ECM, bi-lobed nucleus, bright red granules
note differences in the connective tissue in the lamina propria vs. the submucosa, loose vs.
ln connective tissue beneath villi, round basophilic nucleus, eosiniphilic granules. Don't mi
voluminous, heterogeneous cytoplasm, large cells with irregular shape in the connective ti
Located between Circular and Longitudinal smooth muscle layers of intestine
Cell nuclei are large and pale, round or oval in shape and eccentrically placed
Nuclei smaller than Neuronal Cell bodies
Found in Submucosa (dense connective tissue) near border w/ circular smooth muscle
Larger than neuronal cell bodies in Auerbach plexus
Smaller than glial cells in Auerbach plexus
bright pink
brush border (glycocalyx of apical memb) and mucus droplets of goblet cells are PAS+; bas
single, columnar layer of cells all attached to the basement membrane
Located between Circular and Longitudinal smooth muscle layers of intestine
Cell nuclei are large and pale, round or oval in shape and eccentrically placed
Nuclei smaller than Neuronal Cell bodies
Found in Submucosa (dense connective tissue) near border w/ circular smooth muscle
Larger than neuronal cell bodies in Auerbach plexus
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Smaller than glial cells in Auerbach plexus
Irregular shaped capillaries, filled with erythrocytes
cells with 2 nuclei. Just trying to prove that they do naturally exist.
light cytoplasm and blue zymogen granules
matin and euchromatin, nucleoli
acidophilia is pink (eosin) and basophilia is blue (hematoxylin)
condensed basophilic material along the edge of the nuclear envelope
pyrimidal and arranged in clusters (acini); nucleus usu in basal (peripheral) part of cell
stain less intensely than smooth muscle, wavy bundle near blood vessels
haveperipherally located nuclei and more brightly stained
Centrally located nuclei
Nuclei located in the periphery of cells, stain brighter pink
Long and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi
small diameter, due to transverse section nuclei may appear to be missing
deep cells in epithelium are cuboidal or columnar and superficial cells have convex surface
intense staining at top of cytoplasm
single, columnar layer of cells all attached to the basement membrane
thinly staining line along the top edge of the ciliated cells
secretory unit in which the lumen is greater in sze than the individuals cells.
Irregular shaped capillaries, filled with erythrocytes
Eosinophillic most intense staining, dense fibroblast nuclei
Thin layer of cells surrounding endoneurium, less distinct in longitudial section
lightly stained irregularly spaced connective tissue outside of fasciclePeripherally located basophilic nucleus, cytoplasm eosinophilic (due to preparation
Basophilic, pill shaped
Spaces between schwann cells, multiple slits appear to form X
V-shaped slits
Eosinophillic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie ti
dark staining rings in endoneurium
pale specks in endoneurium around myelinated axons
Appear as empty space in endoneurium
Thin layer of cells surrounding endoneurium, Notice absense of axons
Mostly translucent space with interspersed thick sheaths of connective tissue surrounding f
homogenous intercellular substance; stains blue-gray to purple b/c of sulfates; collagen not
intermembranous bone formation
bone surface, towards outside
along bone surface, large clear nucleus, large cells
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thin, flat cells with nuclei protruding into the lumen of vessels
eosiniphilic, regular pattern, not many cells in it
basophilic nuceli, in the margins of fiber bundlesstellate appearance; note hill and valley appearance of epidermis
contain brownish-gold granules in the upper part of the dermis. Have phagocytosed melan
dark purple outer layer (thin)
dense, eosinophilic layer
loose eosinophilic layer with many clear regions
"hill and valley" organization; basal region of epidermis brown due to melanin in basal kera
projections of dermis into epidermal layer
w/in epidermal pegs; stratified cuboidal epithelium (2 layers) with close nuclei
in dermis near base of epidermisdermis
tall, pseudostratified epithelium
primarily above nucleus
stellate cells in spinous layer with clear halo around nucleus (artifact)
clear cells in basal cell layer
where the large holes are, have huge lipid droplet, little cytoplasm and a flattened nucleus
Thick media
Thin MediaStains blue, connective tissue surrounding the muscle
Stains blue surrounds fascicles
group of cells adjacent to follicles
innermost sheath of follicle
outermost sheath of hair follicle
blue stain around red group of muscle fibers
Purple ring surrounding a group of fascicles
Fascicles located within muscle spindle
Stains intense blue
muscular media of arteries stain red and is much thicker than small veins
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Eosinophilic, multinucleated cells with nuclei at the periphery
lightly eosinophilic connective tissue between muscle fibers
basophilic cluster at periphery of muscle fiber
clear space between muscle fibers
Dark bandsLight bands
located in the center of the I bands
Muscle fiber unit, z-line to z-line
lined with endosteum, which contains osteoprogenitor cells, osteoblasts, and osteoclastscomposed of trabeculae
eosinophilic
large multinucleate cells with abundant cytoplasm, eosinophilic; fewer in number than oste
basophilic
large multinucleate cells with abundant cytoplasm, eosinophilic; fewer in number than oste
along surface of the trabeculae, large cells with single nucleus and basophilic cytoplasm
trapped in lacunae of trabeculae
large amount of adipose tissuemuscle layer
did not survive processing -- mesothelial cells surrounding the heart
arteries have thicker smooth muscle layers than veins
gold colored, near some nuclei
heart muscle
surface layer of endothelial cells, with connective tissue and smooth muscle cells
thicker than normal cardiomyocytes, do not stain as intensely and have fewer myofibrils
in cross section
surface layer of cells and continouls with endothelium of vasculature of valves
observe thick intima, thick media with a lot of elastin, muscle is yellow and collagen is pink
stains black
internal elastic lamina stained black, thin scattered fibers in media; some fibers in adventiti
observe neutorphils, eosinophils and basophils (dark staining granules) in varying stages o
121)
Microtubules disrupted. Cells have multiple unequal nuclei
microfilaments disrupted; binucleate cells b/c cytokinesis unable to occur
anti-desmin probe
positive stain indicates rhabdomyosarcoma (skeletal muscle tumor)
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Stained dark by anti-desmin
Rust color
Blue located on periphery of muscle fibers
Dark Staining
Magenta stained filaments in smooth muscle cells
Nuclei appear corkscrew in shape
Weblike appearance surrounding Smooth Muscle cells
Use Striations to differentiate from Collagenous TendonBrightly stained pink dense connective tissue (lack of striations
thickened
prominent, with deep epidermal pegs
Thick media
Thin Media
surrounding trabeculae of spongy bone
large cells with multi-lobded nuclei that have easinophilic cytoplasm\
outer edge of bone, large cells with basophilic cytoplasm
very outer edge of bone, with basophilic cytoplasm
may see many artifacts where lipid droplets have been extracted
homogenous eosinophilic stain
large, thin-walled vessels lined by endothelial cells and filled with mature blood cells, prima
large cells with multi-lobded nuclei that have easinophilic cytoplasm\
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stain less intensely than smooth muscle, wavy bundle
have centrally located nuclei and more brightly stained
Centrally located nuclei
Thicker Media
larger, Irregular shaped Lumen
Thin,
Contains Longitudinal smooth muscle
bright pink
thick: EC layer plus subendothelial connective tissue
thick, with elastic laminae interspersed with smooth muscle cells
arterioles, cap, and venules in the adventitia
contain black or dark green granules representing residue of material inhaled from the poll
stain brown due to anti-actin stain, located around acinar cellssurrounded by brown staining myoepithelial cells
Look like arterioles with small lumen and no myoepithelial cells
light blue stain
darker blue stain
darker than cytoplasm; basal end of absorptive cells
oval organelles with heterogenous staining; dark areas are nucleoli; dark=heterochromatin
In lower part of the villus among connective tissue, stains dark pruple to black, small and
voluminous, heterogeneous cytoplasm, large cells with irregular shape in the connective ti
ln connective tissue, round basophilic nucleus, metachromatic, purple granules.
as opposed to keratinized stratified squamous, the flattened superficial cells are viable and
Basophilic material in cytoplasm of Neuronal Cell Body
nuclei surrounding neuronal cell bodies
Appear empty in the perfusion fixed sample
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ngement
s a coiled sweat gland below
gate cells with long nuclei
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t microscopy
ape from the basal layer to the surface
omes
omewhat beneath the dermal-epidermal junction
ation
be able to stand out at 10x
paration, pulled away from fibers and appear ragged; pale, clear cytoplasm often with gr
it a wispy appearance; deep blue cytoplasm with ribosomes but no granules
ecursors
th very small azurophilic non-specific granules
mping and curved
rily erythrocytes
tissue
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sue
ascicles
toplasm a frothy appearance.
sue
ascicles
all extend to apical surface
oplets are individually extracted, so the cells skeleton is still visable
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atory response
roblast nuclei
sue
roblast nuclei
elial cells
dense
take for an eosiniphil. It does not have a bi-lobed nucleus
sue at tips of villi
ement membrane will be bright pink line
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roblast nuclei
sue
ascicles
seen b/c forms fine fibrils
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in pigment that escaped from the epithelium
tinocytes; projections of epidermis into dermal region called epidermal pegs or rete bege
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oblasts; look for large eosinophilic cell mass with lots of nuclei
oblasts; look for large eosinophilic cell mass with lots of nuclei
ia mixed with collagen
development
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rily erythrocytes
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uted environment.
, light=euchromatin
niform in size
sue
nucleated
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s
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