RESEARCH GROUP

Cell and Tissue Regeneration

PRINCIPAL INVESTIGATOR

KEY INTERESTSPluripotent Stem Cells • Embryonic Stem Cells • Genomic Stability • Cell Cycle Regulation • Regulation of Cell Differentiation • Tissue Engineering • Bioactive Materials • Cell Authentication

RESEARCH OBJECTIVES Development of MS-based strategies for identification and evaluation of cell phenotypes.

Establishment of clinical grade (GMP quality) lines of human embryonic stem cells and their functional evaluation.

Development of strategies for differentiation of pluripotent stem cells towards bio-medically relevant somatic lineages.

RESEARCH FOCUSThe overall goal of research of The Cell and Tissue Regeneration group (CTR) is to investigate the properties of human stem cells that are relevant to their utility in biomedicine. Particular focus is on pluripotent stem cells, both embryonic and induced, but adult stem cell types are also studied. The biological phenomena that are being studied mainly include: a) genomic stability of stem cells because of its importance to safety in potential clinical applications, b) role of cell cycle regulators and non-coding RNAs in establishing and maintenance of differentiated phenotypes and also in pluripotency, and c) effects of extracellular biodomains on behavior of stem cells, with an emphasis on their contribution to establishment of niche environment. Great effort is also dedicated to understanding of stem cell-to-extracellular matrix interactions and to development of methodologies for supporting growth of cells in engineered scaffolds as well as differentiation of stem cells towards defined somatic cell lineages (e.g. airway epithelia, neural lineage). Lastly, the group is involved in setting standards and rules related to prospective use of stem cells in clinics, via its participation in International Stem Cell Forum and International Stem Cell Banking Initiative.

ST. ANNE‘S UNIVERSITY HOSPITAL BRNOINTERNATIONAL CLINICAL RESEARCH CENTER

Doc. MVDr. Aleš HAMPL, CSc.

Associate Professor at Facultyof Medicine, Masaryk University

E-mail: ales.hampl@fnusa.czPhone: (+420) 549 491 362

CREATING THE FUTURE OF MEDICINE

EDUCATION

1990: CSc./Ph.D. in Animal Physiology and Morphology, Czech Academy of Sciences, Czech Republic

1986: MVDr./DVM at University of Veterinary Medicine, Brno, Czech Republic

TRAINING 1991-1994: Postdoctoral Fellow, The Jackson Laboratory, Bar Harbor, ME, USA

CLINICAL RESEARCH CORE FACILITIESBASIC RESEARCHTRANSLATIONAL RESEARCH

TOP PUBLICATIONS

MAIN PARTNERS AND COLLABORATING INSTITUTIONS Karolinska Institutet, Stockholm, Sweden University of Newcastle, Newcastle, UK University of Cambridge, Stem Cell Institute, Cambridge, UK

OFFERED SERVICES AND EXPERTISELive cell imaging microscopy; transmission and scanning electron microscopy; stem cell phenotyping; establishment, propagation, and differentiation of human pluripotent stem cells; mass spectrometry-based cell fingerprinting, sectioning of normal and engineered tissues for live explant analyses.

BEST RESULTS Contribution to understanding of why and how human embryonic stem cells may develop abnormalities to their genome. These findings create theoretical grounds for development of strategies for safe clinical application of stem cells.

Unraveling of non-coding RNAs that contribute to establishment of cell properties that are typical for pluripotent stem cells. These findings may help to develop strategies for driving cells towards particular functional somatic phenotypes.

Development of methodologies for authentication of cells using mass spectrometry and non-linear analysis by neural networks.

DOLEZALOVA, D., MRAZ, M., BARTA, T., PLEVOVA, K., VINARSKY, V., HOLUBCOVA, Z., JAROS, J., DVORAK, P., POSPISILOVA, S., HAMPL, A. MicroRNAs Regulate p21 Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. Stem Cells. 2012, 30(7), 1362-1372.

BARTA, T., PESKOVA, L., HAMPL, A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Scientific Reports. 2016, 18;6:36625.

VALETTA, E., KUCERA, L., PROKES, L., AMATO, F., PIVETTA, T., HAMPL A, HAVEL, J., VANHARA, P. Multivariate Calibration Approach for Quantitative Determination of Cell-Line Cross Contamination by Intact Cell Mass Spectrometry and Artificial Neural Networks. PLoS One. 2016, 11(1), e0147414.

STREIT, L., JAROS, J., SEDLAKOVA, V., SEDLACKOVA, M., DRAZAN, L., SVOBODA, M., POSPISIL, J., VYSKA, T., VESELY, J., HAMPL, A. A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting. Plastic and Reconstructive Surgery. 2017, 139(3):670e-682e.

VANHARA, P., KUCERA, L., PROKES, L., JURECKOVA, L., PENA-MENDEZ, E.M., HAVEL. J., HAMPL, A. Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells. Stem Cells Translational Medicine. 2018, 7(1):109-114.

St. Anne‘s University Hospital BrnoInternational Clinical Research Center

Pekařská 53, 656 91 Brno | Czech Republic | ID: 00159816Phone: (+420) 543 181 111 | E-mail: icrc@fnusa.cz | www.fnusa-icrc.org

Supported by the project no. LQ1605 Translational Medicine from the National Program of Sustainability II (MEYS CR)

TECHNOLOGICAL EQUIPMENT BioScaffolder 3.1 (GESIM, Germany) – printing wide variety of 3D structures in micrometer dimensions, also with live cells.

VEVO 2100 – high-frequency, high-resolution digital imaging platform with linear array technology and Color Doppler Mode for small animals.

ImageXpress Micro XL (Molecular Devices, USA) – epifluorescence live cell imaging microscopy with high content screening capability.

ImageXpress Ultra (Molecular Devices, USA) – confocal microscopy with high content screening capability.

BASIC RESEARCHExploration of Key Processes for Elimination of Cancer Cells and Selective Manipulation of Stem Cells for Possible Use in Modern Medicine