CELL ADHESION MOLECULES AND MATRIX PROTEINS
-
Upload
shihanas-shibi -
Category
Education
-
view
219 -
download
0
Transcript of CELL ADHESION MOLECULES AND MATRIX PROTEINS
![Page 1: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/1.jpg)
1
CELL ADHESION MOLECULES AND MATRIX PROTEINS
BY
SHIHANA.S FIRST YEAR M.PHARM
DEPARTMENT OF PHARMACOLOGY
![Page 2: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/2.jpg)
2
CELL ADHESION Cell combines to form tissues.
![Page 3: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/3.jpg)
3
TYPES OF CELL ADHESION
• CELL-CELL ADHESION • CELL-MATRIX ADHESION
![Page 4: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/4.jpg)
4
CELL ADHESION MOLECULES (CAMs)
• Cell surface proteins• Cell-cell adhesion may be homophilic or heterophilic interaction• Specificity of cell-cell recognition• Functions-
![Page 5: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/5.jpg)
5
BIOLOGY OF CAMs
• An intracellular domain • A transmembrane domain• An extracellular domain
STRUCTURE OF CAMs
![Page 6: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/6.jpg)
6
CLASSIFICATION OF CAMs
1. Calcium-independent
• IgSF CAMs
• Lymophocyte homing receptors
2. Calcium-dependent
• Integrins
• Cadherins
• Selectins
![Page 7: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/7.jpg)
7
CALCIUM-INDEPENDENT CAMs
1. IgSF CAMs• Homophilic or heterophilic-70-110 amino acids.• Large group of cell surface molecules-VCAM, NCAM, ICAM,
Nectins and Necl.
2. Lymphocyte homing receptors • Addressins
![Page 8: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/8.jpg)
8
CALCIUM-DEPENDENT CAMs
• Integrinso Heterodimeric proteinso Structure o Physiological role
![Page 9: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/9.jpg)
9
• Cadherins o Homophilic interaction-150 aminoacids.o E,N,P o ECD
![Page 10: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/10.jpg)
10
• Selectins
o Heterophilic, bind with mucinso Members-E,L,Po PSGL-1
10
![Page 11: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/11.jpg)
11
ROLE OF CELL ADHESION MOLECULES IN VARIOUS DISEASES
• Inflammation• Ischemic stroke• Leukocyte adhesion deficiency• Alzheimer’s disease• Coronary artery disease• Cancer-metastasis,prostate cancer
![Page 12: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/12.jpg)
12
MATRIX PROTEINS
• Cell shapes, migration, proliferation, cell survival and metabolism.• Consists of protein-carbohydrate complexes.• Biology
![Page 13: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/13.jpg)
13
CLASSIFICATION
1. Proteoglycans
2. Glycoproteins
• Structural proteins- Collagen and Elastin
• Adhesive proteins- Fibronectin and laminin
![Page 14: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/14.jpg)
14
1. Proteoglycans • Core proteins-covalently attached to long non-branched chains of GAGs.• Hyaluronic acids, chondroitin sulfates, dermatan sulfates, heparin sulfate.• Polysaccharide content 95%.
2. Glycoproteins • Short branched oligosaccharides.• Carbohydrate content less than 10%.• Different types of glycoproteins.
![Page 15: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/15.jpg)
15
STRUCTURAL GLYCOPROTEINS
Collagen• 25% of total content, 29 types.• Stiff triple helix-3 peptide chains.• Monosaccharides and disaccharides.• Fibrillar collgens.
![Page 16: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/16.jpg)
16
Elastin
• Does not form stiff triple helix.• Consists of flexible polypeptides.• Microfibrils 10-20nm in diameter-fibrillins.
![Page 17: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/17.jpg)
17
ADHESIVE GLYCOPROTEINS
Fibronectinso Bind with other matrix proteins(strongest binding with collagen-III).o Guide cellular movement.o 20 types
![Page 18: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/18.jpg)
18
Laminin • Cross linked trimeric adhesive proteins.• Flexible-3 polypedtide chain(,,)-disulfide bond.
![Page 19: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/19.jpg)
19
ROLE OF MATRIX PROTEINS IN VARIOUS DISEASES
1. Endocrine diseases• Chronic pancreatitis- pancreatic fibrosis, chronic inflammatory
disease. • Renal hypertrophy
2. Collagenosis 3. Cancer4. COPD5. Infectious diseases
![Page 20: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/20.jpg)
20
REFERENCE
1. Harsh Mohan. Text book of Pathology. 5th edition: 31,137-138.
2. Robbins and Cortan. Pathologic Basis of Disease. 9th edition:72-77.
3. Cyrus C, Jones EY. The Molecular Structure of Cell Adhesion Molecules.
Annual Review of Biochemistry. 1997; 66: 823–62.
4. Alexandros D, Athanasios GP, Michael C, Eleni E, Meletios AD. The Role
of Cell Adhesion Molecules (integrins / cadherins) in Prostate Cancer.
International Brazilian Society of Urology. 2011; 37(3): 302-306.
5. Zerrin S University of Anatolia, Department of Pharmacology. Cellular
Adhesion and Adhesion Molecules. Turkish Journal of Biology. 2001; 25: 1-
15.
6. https://en.wikipedia.org/wiki/Cell_adhesion_molecule.
![Page 21: CELL ADHESION MOLECULES AND MATRIX PROTEINS](https://reader035.fdocuments.in/reader035/viewer/2022062903/58ed61681a28abc8218b4569/html5/thumbnails/21.jpg)
21
THANK YOU