Principios de Analisis Instrumental Skoog Douglas 5ª Ed Capitulo 1 5
CEE 772: Instrumental Methods - UMass Amherst · 2014-12-10 · Mass Spectrometry • General...
Transcript of CEE 772: Instrumental Methods - UMass Amherst · 2014-12-10 · Mass Spectrometry • General...
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CEE 772: Instrumental Methods in Environmental Analysis
Lecture #22 Mass Spectrometry: Chemical Ionization
(Skoog,)
Mercer/Goodwill CEE 772 #22 1
Updated: 10 December 2014
(Harris, Chapt.)
Print version
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David Reckhow CEE 772 #25 2 From: Skoog, 1985
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David Reckhow CEE 772 #25 3 From: Skoog, 1985
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David Reckhow CEE 772 #25 4 From: Skoog, 1985
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Chemical Ionization • Use of reagent gas at
high pressures (0.2-2 torr) – Methane – Ammonia
• Ionize reagent gas • These ions react with
and ionize analyte – Pseudo-M ions
• M-1, M, M+1 – Adduct ions
• M+17, 29, 57
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CI: proton transfer
• Proton transfer will occur from conjugate bases of substances of lower affinity to those higher
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From: Budde, 2001
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CI Spectra
• Spectra of di-isooctylphthalate
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From: Howe et al., 1981, Mass Spectrometry, Principles and Applications
Methane CI
Isobutane CI
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Mass Spectrometry • General References on Instrument Design
– Skoog, Principles of Instrumental Analysis • 1985 (3rd ed): parts of Chapter 18 • 1991 (4th ed): parts of Chapter 18 • 1998 (5th ed): parts of Chapter 20
– Howe, Williams & Bowen, Mass Spectrometry, Principles & Applications
• 1981 (2nd ed): Chapter 1 & 12 – Loconto, Trace Environmental Quantitative Analysis
• 2001: pp356-370 – Budde, Analytical Mass Spectrometry
• 2001, Oxford University Press, – parts of Chapter 1 & 2
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Mass Spectrometry
• MS – molecules are ionized and separated based on the their mass to charge ratio (m/z)
• The molecules are bombarded by electrons and the molecules release 1 or more electrons
• M + e- _> M+ + 2e-
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MS Schematic
David Reckhow CEE 772 #25 10 Figure 18-1, Skoog
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MS Inlet System
• Introduce the sample to the MS • 3 types
– batch inlet – direct probe inlet – chromatographic inlet
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MS - Batch Inlet
• Most common inlet system • Gas or liquid sample is volatized externally,
then leaks into ionization area • A vacuum pressure of 10-4 to 10-5 torr is
applied to the inlet system
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MS – Direct Probe Inlet
• Used for solids and nonvolatile liquids • A sample holder (probe) is used to insert the sample
into the ionization region • A vacuum lock system is used to minimize the
volume of air that must pumped from the system after the probe is inserted in the ionization area
• The probe’s tip is surrounded a heating coil to heat or cool the sample
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MS - Chromatographic Inlet System
• Capillary column output can be fed directly into the ionization region because its flow rate is very small
• Packed column output must flow through a jet separator to remove most of the carrier gas
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Sample Introduction
• General types of devices
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Sample Introduction
• Sample probe
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Interfaces
• Jet Separator
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Ion Source
• Electron Impact
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Ion Source
• Electron Impact
David Reckhow CEE 772 #25 19 From: McLafferty, 1980
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Interface & Ion Source
• Electrospray Ionization
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Ion Source • Electrical Impact Ionization (EI)
– molecules are bombarded with electrons with KE of 70 eV that cause them to be ionized
– EI causes great amount of fragmentation in large molecules causing the mass spectra to be difficult to interpret
• Chemical Ionization (CI) – the ion source is filled with a reagent gas, usually methane, at a
pressure = 100 Pa. CH4 react with electrons to form reactive products such as CH5
+ – CH5
+ reacts with the molecule to form CH4 and MH+ – Softer method – less fragmentation occurs
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• To next lecture
Mercer/Goodwill CEE 772 #22 22