CD11c
description
Transcript of CD11c
AC
D1
1c
BDCA1C
D1
4BDCA3
CD
11
c
MDC8
CD
11
c
CD14
CD
11
c
CD14
CD
11
c
CD14
cDC1:BDCA1+CD11chiCD14-
cDC3: M-DC8+CD11chiCD14lo
cDC2: BDCA-3+CD11c+CD14-
CD
11
c
BDCA2
CD
11
c
CD14
CD
11
c
CD14
pDC: BDCA-2+CD11c-CD123+
monocytes: CD11c+CD14+
BAdditional Figure 2. Peripheral Blood DC (PBDC) gating strategy . A) First, live cells were gated based on FSC-SSC properties of the lymphocyte and monocyte populations (not shown), after which the cDC1, cDC2, cDC3 and pDC populations were identified through BDCA1 (and CD19-, not shown), BDCA3, MDC8 and BDCA2 expression. By backgating on CD11c and CD14 as indicated, the identity of the different DC subsets was confirmed. Activation status of abovementioned cDC, pDC and monocyte subsets was determined by calculating the median Fluorescence Index (med. FI) of CD40 expression by dividing the med. fluorescence (Med. fl) of the CD40 antibody by the med. fl of the isotype-control antibody. B) Isotype control and CD40 histograms are depicted for the cDC1 (left) and cDC3 (right) subsets at week (w) 0 visit (v) 1 (i.e. baseline) and w4v3 for a representative patient.
cDC1
CD40
IgG1
4.6
49.6
5.9
139.5
w0v1 w4v3
cDC3
CD40
IgG1
12.3
58.3
11.0
220.7
w0v1 w4v3
Additional Figure 2