CBB4032 Chemical Analysis Introduction

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Course outline

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Subject : CBB4032 Chemical Analysis

Credit hour: 2

Semester : Sem 2 Yr 4

Status: Major subject

Prerequisite: No

Sypnosis:

Introduction to various types of equipments, Sampling

Method, Gravimetry, Spectrophotometry, Spectroscopy,

Chromatography, Application procedure and interpretation


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1. Apply knowledge of mathematics, science and engineering fundamentals and an engineering

specialisation to the solution of complex chemical engineering problems.

2. Identify, formulate, research literature and analyse complex chemical engineering problemsreaching substantiated conclusions using first principles of mathematics, natural sciences and

engineering sciences3. Design solutions for complex chemical engineering problems and design systems, components or processes

that meet specified needs with appropriate consideration for public health and safety, cultural, societal, and

environmental considerations.

4. Investigate complex chemical engineering problems using research based knowledge and research methods

including design of experiments, analysis and interpretation of data and synthesis of information to providevalid conclusions.

5. Use modern engineering and IT tools to evaluate complex chemical engineering activities.

6. Apply reasoning informed by contextual knowledge to assess societal, health, safety, legal and cultural issues

and the consequent responsibilities relevant to professional engineering practice.

7. Understand the impact of professional engineering solutions in societal and environmental contexts and

demonstrate knowledge of and need for sustainable development.

8. Apply ethical principles and commit to professional ethics and responsibilities and norms of chemical

engineering practice9. Communicate effectively on complex chemical engineering activities with the engineering community and

society.

10. Function effectively as an individual, and as a member or leader in diverse teams and in multi-disciplinary

settings.

11. Recognise the need for, and have the preparation and ability to engage in independent and life-long learning in

the broadest context of technological change.

12. Demonstrate knowledge and understanding of engineering and management principles and apply these toones own work, as a member and leader in a team, to manage projects and in multidisciplinary environments.

Chemical Engineering Programme Outcomes (PO)


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Course outline

Course Learning Outcome (CLO):

At the end of this course, students should be able to

CO1: Analyze data obtained from specific analytical

equipment. Map to PO1/PO2

CO2: To interpret the generated data from specific

analytical equipment. Map to PO1/PO2

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Course outline

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Course

Planning

Topic Hours

Lect. Tut. Lab

Introduction 3

Gravimetry 4 1 #

Spectrophotometry 4 1 #

Spectroscopy 9 2 #

Chromatographic Method 8 2 #

Laboratory Work 8

Total Hours28 6 8


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Course content

1.0 Introduction

Analytical Objectives

Qualitative Analysis

Quantitative Analysis

Type of equipment/

apparatus - Gravimetry,

Spectrophotometry,

Spectroscopy andChromatography

2.0 Gravimetry

Gravimetry Calculations

Properties of Precipitates

Colloidal Precipitates

Crystalline Precipitates

Co-precipitation

Homogeneous Solution

Precipitate Drying

Summary of Methods

Application

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Course content

3.0 Spectrophotometry

Electromagnetic Radiation

Absorption Process



Measuring Absorbance

Emission Process

4.1 Spectroscopy

Atomic Spectroscopy

General Consideration

Atomic Emission

Atomic Absorption

Atomic Fluorescence

Use of X-rays

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Course content

4.2 Spectroscopy

Molecular Spectroscopy

Organic UV-Vis Absorption

Effect of Solvent

Inorganic UV-Vis Absorption



Spectrophotometric Titrations

Molecular Fluorescence

Equipment for MolecularSpectroscopy

5. Chromatography

Principle of Chromatography

Classifications ofChromatoraphic Techniques

Qualitative Analysis Quantitative Analysis

Gas ChromatographyPrinciples and Instrumentation

Liquid Chromatography

Principles and Instrumentation Paper Chromatography and

Thin Layer Chromatography

Hyphenated Methods (GCMS,HPLC, GC-FTIR)

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Grading

Quiz/ Test/ Assignment - 30%

Laboratory/ Project - 20%

Final Examination - 50%

Laboratory Work

Students are required to conduct any four experiments underthe following subjects:

Gravimetry

Fourier Transform Infrared Spectroscopy UV-Visible Spectroscopy

Gas Chromatography/GCMS

High Performance Liquid Chromatography

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Text book

Gary D. Christian, 2003 Analytical Chemistry, 6th Ed., Wiley

Skoog, West, Holler and Crouch, Analytical Chemistry AnIntroduction, 7th Edition, Saunders, ISBN 0-03-020293-0.

Christie G. Enke, 2001. The art and Science of ChemicalAnalysis, Wiley Higher Education. ISBN: 978-0-471-37369-8.

Francis Rouessac and Annick Rouessac, 2005. Chemical Analysis:Modern Instrumentation Methods and Techniques, 2nd Edition,

Wiley Higher Education. ISBN: 978-0-470-85903-2

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Timetable

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Previous Week Printer Friendly Next WeekBack To Selection Wed May 15 14:38:19 2013


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1.0 Introduction

Geranyl acetate

O

CH

Everything is made of chemicals

Elements, substance, molecules

Analytical chemist determinewhat and how much

Investigates, inspect, analyze

Sample characterization for allaspects of material

Analytical chemistry is science

of chemical measurements

12Geraniol
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Objective: Areas in Chemical analysis

Question

Quantitation

Detection

Identification

Separation

Answer

How many substance in

sample? ----------

Does the sample contain Y?--------------

What is the identity of the

substance? ---------

How can interested analytebe separated from sample

matrix? -----------

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What do chemical analyst do?

Analyst

Applies known

measurement techniques to

a well defined

compositional orcharacterization questions

Senior analyst

Develop a new method of

measurement on the

existing principles to solve

new problem

Research analytical chemist

Create or investigate noveltechniques for chemicalmeasurement

Or conduct fundamentalstudies of chemical/physicalphenomena underlyingchemical measurement

Provides methods and tools needed forinsight into material to answer the 4Qsabout a sample:

What

Where

How much

What appearance - structure & form

What is analytical chemistry?

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Qualitative vs quantitative analysis

Qualitative analysisprovides info(identification) about

the identity ofspecies or functionalgroup in the sample

Quantitative analysisprovides numericalinfo of analyte (exactamount orconcentration)

Qualitative analysis is what.

Quantitative analysis is how much.

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Measurement science

Progress in measurement science involve thebasic and targeted research

Research

Targeted

Innovativeconcepts , new

principles

Characteriz

ationanalysis

Specific problem ,novel applications ofexisting techniques

Basic

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Measurement science

Provide tools and eyes to scientist and engineers

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Analytical techniques: Method precision,

sensitivity, selectivity and capability Different method provide different precision,

sensitivity, selectivity and speed capabilities

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Analytical method

Classical methods: Wet chemical methodssuch as precipitation, extraction, distillation,boiling or melting points, gravimetric andtitrimetric measurements.

Instrumental methods: Analyticalmeasurements (conductivity, electrodepotential, light absorption or emission, mass-

to-charge ratio, fluorescence etc.) are madeusing instrumentation.

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Analytical methods

Gravimetric methods determine the mass of the analyte orsome compoundchemically related to it.

Volumetric method, the volume of a solution containingsufficient reagent to react completely with the analyte is

measured.

Electroanalytical methods involve the measurement of suchelectricalproperties as voltage, current, resistance, and quantityof electrical charge.

Spectroscopic methods are based on measurement of theinteraction between electromagnetic radiation and analyteatoms or molecules or on the production of such radiation byanalyte.

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Analytical methodology

Plan

Qualitative & quantitative or both

What kind of info Which technique

Sampling

Accuracy depends on proper sampling

Characteristic of sample is very important

Require good representative sample

Sampleprep

Depends on analytical technique

Type of sample

Analyticalmeasure

ment

The equipments

Data

analysis

The valid data range, replication of analysis

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Consideration in selecting

equipment

Accuracy

Sample amount

Concentration range

Interference

Physical/chemical properties of sample matrix

Number of sample to be analyzed

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Selecting


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Selecting

analytical

methods

An analysisinvolves several

steps and

operations which

depend on:

the particular

problem

your expertise

the apparatus or

equipment

available.

The analyst should

be involved in every

step.

Gary Christian,

Analytical Chemistry,

6th Ed. (Wiley)23


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Established standard

Used established and well recognized

standard or protocol for analysis e.g.:

ASTM

USEPA

BSI

SIRIM

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ASTM

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USEPA

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BSI

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SIRIM

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Summary:

Focus areas

Function of chemical analyst

Qualitative and quantitative

Measurement science

Analytical method

Recognized standard

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CBB4032 Chemical Analysis Introduction

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