Catalog Phenomenex 2012

years of Ingenuity, Imagination, and heart

30Celebrating

Chromatography product guide 12/13

ng brati 30 Yea ele r

30t

f so

Ingenuity, Im a

gination, and H ea r

Lets face it. Chromatography is not sexy, or glamorous.Or is it? From our humble beginnings, with no money and no technology, we set out to create a different type of scientific company. We asked ourselves, Why do we have to be another boring chromatography company? Could we reinvent approaches in research, customer care, and employee welfare? You see, when you start off as the underdog, you can either choose to copy or decide to approach everything differently. We, of course, chose to rethink everything. Our slogan, breaking with tradition was born from that thought process and it still shapes our decisions today. We are proud we took the more difficult path as we are confident the results are more ingenious, more imaginative, and full of more heart than would have ever been possible if we had just followed the (former) leaders of the pack. Because it has been 30 years, we figured this milestone would be a good opportunity to share a little more about who we are, what we stand for, and to see if you think were on the right track. After all, without YOU, who are we? Lets get started! In the past three decades

We advanced.And by advanced, we mean technologically. Over the years, we have introduced cutting-edge technologies and services that have changed the face of separation science. Just a few highlights...

1982 We open our doors as an HPLC column distributor

2012 Phenomenex, Inc. All rights reserved.

C2007

1986

1988 1990

1986 We became an HPLC column manufacturer

1996 Introduce Luna which quickly becomes the worlds #1 HPLC Column 1997 Acquired GC column manufacturing1998 1999

2002

2000 Diversified into SPE manufacturing

2006 Clarity BioSolutions for Synthetic DNA/RNA

2004Free app for your mobile: http://www.gettag.mobi

2008

2009 Introduced the first and only sub-2 m core-shell technology

2010

2011 Applied market-leading core-shell technology for BioSeparations and introduced PhenoLogix method development services

The Future Many more exciting technologies in our pipeline for years to come!

2012

We expanded.Back in the fluorescent days of 1982, we started selling columns out of a garage. Over the course of time, we slowly and strategically planned how and where we would expand. By 2011, we had opened 3 manufacturing sites, spread into 10 buildings at our California headquarters, opened 15 international subsidiary offices (including our newest in Hyderabad, India), and partnered with over 65 distributors to ensure worldwide availability of our products. And what about the future you might ask? We plan to accelerate our growth even more!

Corporate OfficeMr. Mahjoors Garage. Circa 1982

Buildings 1, 2, 3, 5, 6, 7, and 10 (shown). Present day.

Corporate Offices

Italy

Germany

France

India

Australia

UK

Denmark

The Netherlands

New Zealand

InventX (GC Manufacturing)

Wibby Environmental

For the sake of humanity, visit page 60.Phenomenex1

Dear M

ustom Its ha er, rd to im agine that rid that 3 iculou 0 yea sly go excep rs hav od-loo t a lot e pas king y sed. I better journe oung most lookin y with man t certain g now hat st many have ly don ! But s arted positiv overc t feel erious Pheno e acc ome. any d ly, its mene ompli ifferen been x in a shme But to t from an am nts an garag be qu azing e in 1 d natu ite ho and m we ha 982, rally s nest, ost gr ve co ome o I belie atifyin me a bstac ve tha later, long w g les, all t at th we co ay sin of wh e core ntinue ce ou and h ich we , we a to live r hum uman re still up to ble be itya a you our fo ginnin nd mu ng co undin gs. Ye Coun ch mo mpan g mis ting y t, Im re inte y. Cer sion ou am deligh ntly th tainly, amaz to ser ong o ted to an ev es (an ve ou ur cus note 3 er befo d exh r cust tomer develo 0 yea re. austs) omers s is so rs ping li me to , emp methin fe-imp loyees keep ronme g for w roving up wit , nt and hich w therap h all t food q more e are he bril eutics uality. efficie extrem liant w , to th My pr nt pro ely pr e grea ork yo omise ducts oud. It t mea u do e Betwe to you to sup sures ach d en yo port y is tha you ta ay, fro u and t we w our ne ke to our gr m me, w ver-en ill con protec eates hen re tinue ding s t achie t our e with t flectin to des cientifi veme nvialente g on t ign be c prog nts w d, cre he pa tter an as bu ress. From ative, st thre ilding d the m and fu e dec a com ultitud ades, n-lovin humo pany es of I mus ur, we g emp cultur huma t say take g e fuell loyees nitaria this e that o ed by reat c in ma n effo nviron ne of are in passio ny co rts an ment provid tions. untrie n, and d team was b Australia s arou Of co ing an filled orn m adven urse, nd the atmos any a tures by the I have world phere wardto the best, to info . winnin that n Austria daily in most rm yo inspir ourish g tech passio jectio e me, u that es an nolog ns of nate, they m I cons d insp ies an Belgium caring to wo ider m ake life ires. O d cus rk har , loyal, yself s tomerut of at Phe der fo diligen progre o priv focus r their nome Canada t, and ileged ed so ss to nex so benefi innov lumy co to be t, too. exhila ative c lleagu surrou Denmar rating I give My ho olleag es eve nded k , and all the ues. T pe is rywhe therefo credit that y hey re. I re ality o re, I s for all ou ex Finland main f our P ee it a perien of our indeb henom s my ce the accom please ted to duty enex deep plishm d eac France them, family comm h and ents a as I d , beca itmen every nd o to y use it Pheno t and time y ou. German brings wond mene y ou wo erfully me gr x reall rk wit yours eat ple y is a refres h us. elf? B India scient hing p asure ut I w ific co erson prepa know arn yo mpan ing th red to u, the Ireland y like at you join in re is a no oth are the fu lways We w er. Wh n! some ish yo y don thing Italy u grea t you going t happ come on be Luxem iness and s hind t bourg and h ee for hese ealth doors Fasha in 201 . So b Mexico Mahjo 2. e or CEO a nd Pre Nether lands siden Pheno t mene New Zea x land Norway Puerto Rico

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drid Av e Torranc nue e, 90501-1 CA 430 USA Tel: 31 0-212-0 Fax: 31 5 Email: 0-328-7 55 info@p 768 he www.p nomenex.com henom enex.c om

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Sweden United Kingdom USA

2

Phenomenex

SERVICES FIltRatIon

58

9 18

Contents

Table of

PIPEttES VIalS SamPlE PREPaRatIon / SPE GC ColumnS and aCCESSoRIES HPlC uHPlC BIoSEPaRatIonS

19 20

21 34

35 59

61 143

144 264

265 276 277 310

Amino Acids ..........................................................................278 282 Proteins / PePtides .............................................................283 300 synthetic dnA / rnA ............................................................301 310

CHIRal lC SuPERCRItICal FluId CHRomatoGRaPHy (SFC) PREP ColumnS and Bulk mEdIa aCCESSoRIES and ConSumaBlES aPPEndICES

311 326

327 333

335 352

353 378

379 408

Our

MissionThe very existence of Phenomenex depends directly on the complete satisfaction of our customers. Therefore, it is our duty to acknowledge the contributions made by our customers and our staff toward the progress of the company. Further, it is our responsibility and foremost mission to promote the growth, prosperity, and wellbeing of those we serve our customers, our employees, and humanity. Fasha Mahjoor CEO and Founder

Foundingof Phenomenexs CEO The oneness of humankind

Beliefs and PrinciplesThe abolition of all forms of prejudice such as those based on race, gender, religion, nationality, or class Upholding equal opportunity, rights and privileges for men and women The essential harmony of scientific and spiritual truths, which could constitute the ultimate basis for a peaceful, ordered, and progressive society Universal compulsory education for all children Economic justice Protection of human rights Protection of endangered species Protection of the environment

4

Phenomenex

services

5-8

Services

ServicesPhenoLogix .............................................................................................. 6

ServicesPhenoLogix:phenologix | services

A NEW Analytical Support Laboratory Personalized Solutions Experienced Staff Fast Project Turn Around Time Confidential Immediate Availability Award Winning Customer Service PhenoLogix is a full-service analytical support laboratory within Phenomenex that specializes in method development and optimization for HPLC, UHPLC, GC, GC/MS, LC/MS/MS, and SPE. Our team of highly experienced scientists provides customized solutions to help streamline your development efforts. PhenoLogix is committed to making the experience of working together easy, effortless, and rewarding. Get started today, and let us exceed your expectations.

We are as passionate about science as you are, and treat your projects as we would our own. Jeff Layne, Ph.D.

Technical Manager, PhenoLogix

6

Phenomenex

ServicesHow Can We Be of Service?HPLC | UHPLC | GC | GC/MS | LC/MS/MS | SPE | GFC | SFC | CHIRAL | GPC | IEX | IC New Method Development and Optimization Method Improvement (Without the Need for Re-Validation) Validation Services, Including FDA Compliant cGMP Validations Chiral Screening and Optimization Analytical to Preparative HPLC Scale Up Small Scale Purification (< 2 g) Hands-On Training Consulting On-Site Method Transfer PhenoLogix offers different levels of support based on your companys needs, ranging from simple column screening services to full FDA compliant cGMP validations. We are also more than happy to send one of our scientists to your lab to help solve a problem hands-on or to provide training seminars and professional consulting services. A majority of our service offerings are free of charge. For those requiring an additional cost, pricing will be disclosed and approved prior to beginning a project. services | phenologix

Phenomenex

7

ServicesGet Started Today We Make it Easyphenologix | services We guarantee a great experience when working with our team. All of our project managers are enthusiastic and dedicated to you, our valued customer.

Contact Us1Speak with one of our friendly PhenoLogix team members or log onto our website at www.phenomenex.com/phenologix USA/CAN/MX: [email protected] EUROPE: [email protected] ASIA: [email protected] Confidentiality If necessary, put a confidentiality agreement and/or other documentation into place.

Tell Us About The Project2Provide information on your project such as compound structure, specific requirements, and separation goals.

Most Services are Free If a cost is involved, you will receive a customized plan, pricing, and timeline for each individual project for your approval.

Tell Us When to Start3Approve our proposal and well get to work on your project!

Cool Mascot

Jix,

Open Lines of Communication While working on your project, we will hold regular meetings to update you on our progress until you are satisfied with the final method.

Project is Complete4A detailed report is sent to you, including full method, materials, chromatograms, and discussion. Method Transfer We make sure the method works in your laboratory either by supporting your efforts via teleconference or sending a PhenoLogix representative to your lab. We dont finish until you are happy!

5

Relax! Wonder what Im listening to? www.phenomenex.com/phenologix

8

Phenomenex

Filtration Products

9 - 18

Sample Handling and Mobile Phase

Filtration

Filtration ProductsCentrifugal Filters ................................................................................... 12 Column Couplers ................................................................................... 18 Filter Membranes ................................................................................... 15 Inlet Filters Metal-free / Biocompatible ............................................................... 16 Stainless Steel .................................................................................. 16 In-Line Filters Biocompatible PEEK ................................................................... 17-18 Stainless Steel ............................................................................. 17-18 Mobile Phase Filtration Accessories....................................................... 14 Mobile Phase Filtration System .............................................................. 13 Solvent Pickup Adapter .......................................................................... 14 Syringe Filters Non-sterile ................................................................................... 10-11 Sterile................................................................................................ 12 Syringes, Disposable .............................................................................. 12

FiltrationSyringe FiltersPhenex

best seller

For sample and solvent filtration prior to chromatographyFiLTrATiON - SAMple hAnDling Rapid filtration of HPLC and GC samples prior to analysis Particulate, PVC, and extractable-free filters Less system downtime More consistent, reproducible results Increased column lifetime Phenex Offers: Broad chemical compatibility Minimized extractables excellent flow rate high total throughput Certified quality 100 % integrity tested low hold-up volume low protein adsorption Bi-directional use

Syringe Filter Selection Guide1. Choose filter diameter based on sample volume4 mm Diameter 2 ml Sample Volume 15 mm Diameter 2 - 10 ml Sample Volume 25 - 28 mm Diameter 10 - 100 ml Sample Volume

SYRinge FilTeRS

|

2. Choose a pore size based on the nature of your sample and chromatographic methodsample Description general aqueous or mixed organic samples prior to hplC analysis with columns packed with > 3 m particles. general clarification of gC, SFC, Ce, and gpC samples. Viscous samples or samples containing high levels of particulate matter. general aqueous or mixed organic samples prior to hplC analysis with columns packed with < 3 m particles. Removal of fine particulate matter prior to gC, SFC, Ce, and gpC samples. gas samples prior to gC. liquid samples prior to UhplC or lC/MS. other particulate-sensitive methods. Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such as some environmental, biofuels or food and beverage applications. Glass Fiber Filter with 0.45 m filter 0.20 m recommended Filter Pore size 0.45 m

3. Choose a filter membrane according to the characteristics of your sample and filtering objectiveMembrane Typerc

recommended Uses hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions, making them one of the most universal filter materials used prior to chromatography. phenex-RC filters also exhibit fast-flow and ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, phenex-RC membranes are broadly recommended as an excellent general purpose/high-performance sample filter for most applications. pTFe is an inherently hydrophobic membrane excellent for filtration of organic-based, highly acidic or basic samples and solvents. Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water. polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many life science clarification applications. phenex-peS membranes typically offer better chemical resistance than cellulose acetate and are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers. nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples. in combination with a glass pre-filter (phenex-gF/nY), this membrane is excellent for the filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. For applications that require low protein or non-specific binding characteristics, phenomenex recommends phenex-RC (Regenerated Cellulose) filters. Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. in combination with a glass pre-filter (phenex-gF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification. phenex-gF (glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 m pore size. They are commonly used with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth samples, removal of yeasts, molds, etc.). glass Fiber filters can be used alone or in series with other phenex filter membranes such as the 0.45 m pore phenex-RC filter to reduce clogging of the membrane and optimize flow.

(Regenerated Cellulose)

PTFe, Teflon

(polytetrafluoroethylene)Pes

(polyethersulfone)NY

(nylon)

cA

(Cellulose Acetate)GF

(glass Fiber)

10

Phenomenex

FiltrationSyringe Filters (contd)syringe Filter Applications and recommended MembranesApplication / sample hplC and gC Sample prep Aggressive or pure organic Solvents protein Analysis / Biological Samples high particulate loads environmental Methods Food and Beverage Clinical / Toxicology Dissolution Testing ion Chromatography Trace Metals (iCp-MS, AAS) Capillary electrophoresis (Ce) Tissue Cultures, Media, Buffers Ordering Information Membrane Type/size 0.20 m phenex-RC (Regenerated Cellulose) phenex-peS 3 (polyethersulfone) phenex-pTFe 6 (polytetrafluoroethylene) phenex-nY (nylon) phenex-gF/nY 2 (glass Fiber/nylon) phenex-gF/CA 2,3,4 (glass Fiber/Cellulose Acetate) 0.45 m phenex-RC (Regenerated Cellulose) phenex-peS 3 (polyethersulfone) phenex-pTFe 6 (polytetrafluoroethylene) phenex-nY (nylon) phenex-gF/nY 2 (glass Fiber/nylon)1

recommended Filter RC pTFe peS gF/nY gF/nY gF/nY RC gF/nY RC RC RC gF/CA

First AlternativepTFe RC RC gF + RC RC RC peS RC peS peS peS peS

second AlternativepeS nY gF/CA pTFe pTFe pTFe nY pTFe pTFe nY nY RC

if phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, return the product with comparative data within 45 days for a FUll ReFUnD.

SAMple hAnDling - FiLTrATiON

Tip: Try a Sample Pack! Request yours today by phone or visit www.phenomenex.com/sampleFor high load and particulate-laden samples you may consider placing a Glass Fiber (GF) pre-filter, either integrated with the membrane as one unit (Phenex-GF/NY or -GF/CA) or in series with the membrane syringe filter of your choice. 25 - 28 mm Diameter for 10 100 mL sample volumes Part No. AF0-8203-12 AF0-8203-52 AF0-8208-12 AF0-8208-52 AF0-1202-12 AF0-1202-52 AF0-1207-12 AF0-1207-52 AF0-1A47-12 7 AF0-1A47-52 7 AF0-8A09-12 AF0-8A09-52 AF0-8103-12 AF0-8103-52 AF0-8108-12 AF0-8108-52 AF0-1102-12 AF0-1102-52 AF0-1107-12 AF0-1107-527 7 5 5 7 7

Phenex Syringe FiltersPart No.

4 mm Diameter for 2 mL sample volumes Unit 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk Price

15 mm Diameter for 2 10 mL sample volumes Part No. AF0-2203-12 AF0-2203-52 AF0-2202-12 AF0-2202-52 AF0-2207-12 AF0-2207-52 Unit 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk Price

|

Unit 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk

Price

SYRinge FilTeRS

AF0-3203-12 AF0-3203-52 AF0-3202-12 AF0-3202-52 AF3-3207-12 AF3-3207-52

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a nylon (nY) membrane. excellent for filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most difficult samples. outlet connection is luer-lock. An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. excellent for filtration of tissue culture media, general biological sample filtration and clarification. outlet connection is luer lock. AF0-3103-12 AF0-3103-52 AF0-3102-12 AF0-3102-52 AF3-3107-12 AF3-3107-52 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk AF0-2103-12 AF0-2103-52 AF0-2102-12 AF0-2102-52 AF0-2107-12 AF0-2107-52 100/pk 500/pk 100/pk 500/pk 100/pk 500/pk

5 5 7 7

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a nylon AF0-1B47-12 7 (nY) membrane. excellent for filtration of particle-laden samples, such as foods and beverages, AF0-1B47-52 7 environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most difficult samples. outlet connection is luer-lock. An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. excellent for filtration of tissue culture media, general biological sample filtration and clarification. outlet connection is luer lock. prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. outlet connection is luer lock.7. 28 mm diameter.

phenex-gF/CA 2,3,4 (glass Fiber/Cellulose Acetate) 1.20 m phenex-gF 2,3 (glass Fiber)

AF0-8B09-12 AF0-8B09-52

7 7

AF0-8515-12 AF0-8515-52

7 7

100/pk 500/pk

1. Larger quantity purchases at significant savings are available. 2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 m. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite. 4. Cellulose acetate is surfactant-free. 5. 26 mm diameter. 6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.

8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance.

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP), and offer luer lock inlet/slip outlet connections, unless otherwise indicated.

Phenomenex

11

FiltrationSterile Syringe FiltersSterile syringe filters from Phenomenex are ready-to-use, individually blister packaged units, offering high flow rates at low inlet pressures, for rapid sterile filtration.

Centrifugal FiltersPhenex

Disposable Centrifugal Filter Convenient filtration of multiple HPLC and GC samples High recovery for small samples Nylon, PTFE (Teflon) and Cellulose Acetate membrane materials

| FiLTrATiON - SAMple hAnDling

DiSpoSABle SYRingeS / STeRile SYRinge FilTeRS / CenTRiFUgAl FilTeRS

Ordering Information sterile syringe Filters Pore Disc Part No. size (m) Diameter (mm) AF0-8455 0.2 28 AF0-8456 0.45 28 AF0-8457 0.2 28 AF0-8458 0.45 28 AF0-8459 0.2 25 AF0-8460 0.45 25 AF0-8461 0.2 25

Membrane Material CA luer/Slip CA luer/Slip peS luer/Slip peS luer/Slip RC luer/Slip RC luer/Slip pTFe luer/Slip

Unit 50/pk 50/pk 50/pk 50/pk 50/pk 50/pk 50/pk

Price

Phenex disposable centrifugal filter units provide a convenient means of filtering multiple HPLC and GC samples without the necessity of manually forcing each sample through the filter with a syringe. All you need is a laboratory centrifuge with sufficient capacity for the number of samples to be filtered at one time. Centrifugal force drives the sample through the filter quickly without effort on the part of the chemist. No cleaning of syringes is required between samples. Samples contact only the Phenex filter unit itself and the polypropylene receiver tube. After filtration the upper portion of the tube containing the membrane filter can be discarded. The receiver tube serves as a container for the filtered sample and can be retained as long as desired. Separate caps are supplied for the sample chamber and the receiver tube. Filter membrane materials available are Nylon 66, PTFE (Teflon) and Cellulose Acetate (CA). Nylon 66 Phenex filter units are recommended for most HPLC and GC sample filtration work. They are chemically resistant, compatible with all commonly used solvents, and virtually free of extractable materials that could contaminate samples. PTFE (Teflon) Phenex filters are designed to handle more aggressive organic solvents, whereas Cellulose Acetate (CA) is especially well-suited for the filtration of proteinaceous samples in aqueous solvents.

All-Plastic Disposable Syringes Use for all syringe filter applications Luer-lock outlet makes connection easy Capacities ranging from 3 to 20 mL Made of ultra-clean, high-purity plastics

Ordering Information All-Plastic Disposable syringes Part No. Description As0-8408 plastic Disposable Syringes, luer-lock As0-8409 plastic Disposable Syringes, luer-lock As0-8410 plastic Disposable Syringes, luer-lock As0-8411 plastic Disposable Syringes, luer-lock

capacity (mL)* 3 5 10 20

Unit 100/pk 100/pk 100/pk 100/pk

Price

Ordering Information centrifugal Filter Units Pore volumes (mL) Membrane Part No. size (m) sample/receiver Non-sterile AF0-0438 0.2 2.0 / 5.0 nylon AF0-0439 0.45 2.0 / 5.0 nylon AF0-0440 0.2 2.0 / 5.0 pTFe AF0-0441 0.45 2.0 / 5.0 pTFe AF0-8353 0.2 2.0 / 5.0 CA AF0-8354 0.45 2.0 / 5.0 CA

Unit 25/pk 25/pk 25/pk 25/pk 25/pk 25/pk

Price

* Choose larger volume syringe to reduce force on syringe filter membrane. 10 mL syringe is recommended.

Above centrifugal filter units are non-sterile.

12

Phenomenex

FiltrationAll-Glass Vacuum Mobile Phase Filtration SystemFilterSys Prevents pump and system component damage Rapid filtration of buffers, organics and corrosive liquids Removes damaging microparticulates and bacterial contaminants HPLC and GC solvent and sample filtration, 1 to 4 L units Designed for rapid filtration of particulate matter from HPLC solvents, this unit is an excellent value. Protect your instrument and column from costly damage by clarifying all your HPLC solvents and buffer solutions before use. This vacuum filter assembly comes with a sample reservoir and receiving flask. A 47 mm diameter membrane filter is placed between the fritted-glass support base and the sample reservoir, secured in place by an aluminum clamp. The support base itself is connected to the receiving flask by a vacuum-tight ground-glass joint. Only low-extractable borosilicate glass and the membrane filter come into contact with the mobile phase. The vacuum hose connection is made above the filtration drip tip to prevent contamination from the vacuum line. Recommended filter membranes: Nylon 66 is a highly resistant material and can be used with almost all laboratory solvents. Since Nylon 66 is hydrophilic, no prewetting of the filter is required. PTFE (Teflon)membrane filters are excellent for organic or other aggressive solvent systems. Extremely low levels of extractables (plasticizers, contaminants, etc.) make this an excellent filter for trace analysis work. PTFE is hydrophobic, so it is not recommended for the filtration of aqueous solutions.Ordering Information Mobile Phase Filtration system Part No. Description complete Assembly Ah0-1566 FilterSys, 47 mm, 300 ml funnel with 1 l vacuum flask Ah0-3314 FilterSys, 47 mm, 500 ml funnel with 2 l vacuum flask Ah0-3315 FilterSys, 47 mm, 1000 ml funnel with 4 l vacuum flask component Parts Ah0-1567 Fritted support base, 47 mm, 40/35 taper Ah0-1568 Funnel, graduated, 300 ml, 47 mm Ah0-3323 Funnel, graduated, 500 ml, 47 mm Ah0-3324 Funnel, graduated, 1000 ml, 47 mm Ah0-1569 1 liter filter flask, 40/35 taper Ah0-3321 2 liter filter flask, 40/35 taper Ah0-3322 4 liter filter flask, 40/35 taper Ah0-1570 Aluminum clamp, 47 mm Filter Membranes AF0-0503 nylon 66, 0.2 m, 47 mm AF0-0504 nylon 66, 0.45 m, 47 mm AF0-0513 pTFe, 0.2 m, 47 mm AF0-0514 pTFe, 0.5 m, 47 mm

MoBile phASe - FiLTrATiON

Graduated Funnel

Solvent Pickup Adapter Compression Fitting Connector (to Solvent Source)

Valve

| MoBile phASe FilTRATion SYSTeM

Clamp

Phenex Filter Membranes

Unit ea ea ea

Price

to Vacuum Line

Fritted Glass Support Base

Filter Flask

ea ea ea ea ea ea ea ea 100/pk 100/pk 100/pk 100/pk

WARNING: The apparatus should be used with a water aspiration line, not a true vacuum line, unless secured behind an appropriate safety shield.

For compatible Solvent Pickup Adapter, see p. 14 For additional Filter Membranes, see p. 15 The following cross-reference chart will help you find the right apparatus and components as compared with competing products, and should save you time and money overall.

FilterSys Filtration Apparatus Cross-Reference ChartPhenomenex Alltech Kontes rainin supelco Waters Whatman Wheaton

Complete Assembly 47 mm, 300 ml funnel with 1 l vacuum flask Fritted glass Support Base, 47 mm, 40/35 ST Funnel, graduated 300 ml, 47 mm 1 liter Filter Flask, 40/35 ST Aluminum Clamp, 47 mm Solvent pickup Adapter, 47 mm* N/E - No Equivalent Available.

Ah0-1566 Ah0-1567 Ah0-1568 Ah0-1569 Ah0-1570 Ah0-2947

n/e* n/e* 350064 n/e* 350061 2343

953825-0000 953826-0000 953751-0000 953827-0000 953753-0000 953904-0000

419380 419382 419350 635525 419354 S-953906

Z29,040-8 Z29,060-2 Z29,052-1 Z29,061-0 Z29,056-4 Z29,059-9

xx15 047 00 xx15 047 02 xx10 047 04 xx15 047 05 xx10 047 03 n/e*

n/e* n/e* 809C0010 n/e* 816C0045 n/e*

419380 419382 419350 635525 419354 419620

Phenomenex

13

FiltrationSolvent Reservoirs/Bottle Filter Cap Eliminates makeshift HPLC reservoir covers, such as aluminum foil, Parafilm, etc. Neatly seals to prevent particulate contamination FiLTrATiON - MoBile phASe Minimizes solvent evaporation and gas absorption A disposable filter (any size Luer lock Teflon or nylon syringe filter) on the pressure equalization port minimizes the reabsorption of dissolved gases and prevents particle contamination. These assemblies fit the standard screw cap bottles with 38 mm cap size. The 1/8 in. OD Teflon feed line from the cap attaches directly to the HPLC pump. All cap parts are compatible with most common HPLC solvents.

Solvent Reservoir and Reagent Bottles Popular 1 and 2 liter sizes, equipped with 3-way valve cap Low-leaching (low alkali), borosilicate glass Chemically inert, internal PTFE seal These wide-mouth GL45 mobile phase reservoirs come in the two most popular 1- and 2-liter sizes. The reservoirs have easy-to-read volumetric markings to indicate the amount of solvent remaining. The versatile 3-way valve cap supplied with each reservoir provides a totally-inert PTFE (Teflon) seal against the solvents inside. A 2-way valve can be ordered separately.

MoBile phASe ACCeSSoRieS

|

Ordering Information Filter cap Part No. Description Ah0-1565 Filter Reservoir Cap

cap size 38 mm

Unit ea

Price

Ordering Information reservoir and valve cap Assembly Part No. Mfr. No. Description Ah0-4142 3200 hplC Reservoir, 1000 ml clear glass, gl45 wide-mouth, includes 3-way Valve Cap Ah0-4143 3203 hplC Reservoir, 2000 ml clear glass, gl45 wide-mouth, includes 3-way Valve Cap

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Replacement Valve CapsFor GL45 Neck Mobile Phase Reservoirs Supplied with 1/8 in. fitted tubes, will not twist when tightened Two cap types (polypropylene outer cap or all-Teflon block design) Replacement 2- and 3-Valve Caps are available to fit the GL45threaded type of mobile phase reservoir offered on this page. The valved caps provide an inert PTFE (Teflon) seal against the solvents inside, and two types are available depending on construction of the outer cap: either polypropylene or all-Teflon block design with stainless steel heli-coils to grip the Teflon tubing. Select the polypropylene type if chloride salts or strong acids or bases are being used. Valve caps are pressure rated to 15 psi (1 bar) and are autoclavable.

Solvent Pickup AdapterThis glass adapter enables direct pickup of mobile phase solvent for filtration using the Phenomenex FilterSys (see previous page). Safe in-line filtration with the pickup adapter replaces the tedious and dangerous pour-and-wait funnel filtration method. Eliminate the possibilities of spilling solvents and breathing toxic vapors. Replacing the funnel adapter on the FilterSys unit, the 47 mm pickup adapter with flange holds the membrane filter in place on top of the fritted support base, which in turn is held by the aluminum clamp (not included). The pickup adapter draws solvent directly from the reagent bottle the safest way to transfer and filter solvents. The adapter includes a 4 mm PTFE (Teflon) valve with in. OD outlet, PTFE 90 elbow with compression fittings for in. OD tubing, and 3 feet of in. OD PTFE tubing.

Ordering Information replacement valve caps for GL45 Threaded Bottles* Part No. Description Ah0-8599 2-Way Valve Cap, gl45 threads, with polypropylene outer cap Ah0-4141 3-Way Valve Cap, gl45 threads, solid pTFe (Teflon) block construction, with tubing (1/8 in. oD)* Fittings not included. See p. 364 AQ0-2950

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Price

Ordering Information solvent Pickup Adapter Part No. Description Ah0-2947 Mobile phase pickup Adapter, 47 mm

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14

Phenomenex

FiltrationAdditional Filtration ProductsFiltration Products from PhenomenexFiltration is the easiest way to improve your results, guard your system components from damage, and protect your column investment. Regular sample filtration means: Less system downtime Fewer troubleshooting problems Removal of particulate matter to sub-micron levels is critical before any drug, tox, or dirty environmental sample is injected into an HPLC, GC or mass spectrometer. Also, products that remove matrix components, interferents, and chemical garbage will improve your results. Check out the following useful products:

Filter MembranesPhenex RC, Nylon, PTFE (Teflon), and other membranes available Wide selection of membrane sizes Phenex PTFE (Teflon) and Regenerated Cellulose (RC) membrane filters offer excellent chemical resistance to almost all laboratory solvents and samples. They do not introduce unwanted plasticizers or extractables into the sample or mobile phase. Since Regenerated Cellulose (RC) is hydrophilic, filtering of aqueous solvents is simple. No prewetting is required. PTFE is hydrophobic and so is not recommended for the filtration of aqueous solutions.Ordering Information Filter Membranes Pore Disc Part No. size (m) Diameter (mm) Nylon AF0-0500 0.45 13 AF0-0501 0.2 25 AF0-0502 0.45 25 AF0-0503 0.2 47 AF0-0504 0.45 47 AF0-0505 0.2 90 AF0-0506 0.45 90 PTFe AF0-0509 0.2 13 AF0-0511 0.2 25 AF0-0512 0.45 25 AF0-0513 0.2 47 AF0-0514 0.45 47 cellulose Acetate (cA) AF0-8434 0.45 13 AF0-8435 0.2 13 AF0-8436 0.45 25 AF0-8437 0.2 25 AF0-8438 0.45 47 AF0-8439 0.2 47 regenerated cellulose (rc) AF0-8440 0.45 13 AF0-8441 0.2 13 AF0-8442 0.2 25 AF0-8443 0.45 47 AF0-8444 0.2 47 Polyethersulfone (Pes) AF0-8445 0.2 25 AF0-8446 0.45 25 AF0-8447 0.2 47 AF0-8448 0.45 47 cellulose Nitrate ester (Mce)* AF0-8449 0.80 13 AF0-8450 0.45 13 AF0-8451 0.80 25 AF0-8452 0.45 25 AF0-8453 0.80 47 AF0-8454 0.45 47

FiLTrATiON | FilTeR MeMBRAneS / ADDiTionAl pRoDUCTS

Mini-index

Page No.

Membrane Material nylon nylon nylon nylon nylon nylon nylon pTFe pTFe pTFe pTFe pTFe CA CA CA CA CA CA RC RC RC RC RC peS peS peS peS MCe MCe MCe MCe MCe MCe

Unit 100/pk 100/pk 100/pk 100/pk 100/pk 5/pk 5/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk

Price

Filtration Products (General Laboratory) Syringe filters ................................................................ 10-12 Membrane filters and filtration apparatus ......................13 Centrifugal filters ...............................................................12 Solid Phase Extraction (SPE) devices Tubes and well plates ...........................................................35-56 Vacuum manifolds ................................................................57-59 Column Protection devices SecurityGuard UhplC/hplC (2.1 to 4.6 mm iD) .................................................239 Analytical (2 to 8 mm iD) .................................................... 235-239 Semiprep (9 to 16 mm iD) .................................................. 237-238 pRep (18 to 29 mm iD) ...................................................... 237-238 pRep (30 to 49 mm iD) ...................................................... 237-238

Inlet filters Biocompatible peeK ....................................................................16 Stainless steel .............................................................................16 In-line filters Biocompatible peeK Analytical ................................................................................17 Semiprep ................................................................................18 Stainless steel Analytical ................................................................................17 prep ........................................................................................18 Quality filtration and sample preparation products from Phenomenex offer a convenient, inexpensive way to clarify all your samples for peace of mind and improved analytical results.

*MCE = Mixed Cellulose Esters Above filter membranes are non-sterile. Phenex is a trademark of Phenomenex, Inc.

For a useful Membrane Selection Guide, see p. 10

Phenomenex

15

FiltrationInlet FiltersStainless Steel Protects pumps and check valves Easy to replace FiLTrATiON - MoBile phASe Low cost HPLC solvent inlet filters are used at the low pressure inlet side of the pump to help protect the check valves, injector and column from damaging particulate contamination. Solvent filters are constructed of Hastelloy Steel and are available for 1/16 in. ID and 1/8 in. ID tubing. Due to the large surface area of the cylindrical frit, virtually no backpressure or cavitation is developed. The filter is easily cleaned by backflushing or sonicating.

Metal-Free/Biocompatible Biocompatible Flat bottom design uses all available mobile phase High surface area for long filter life The Solvent Saver Inlet Filter Unit utilizes a flat filter element that sits parallel to the bottom of the HPLC reservoir. The design allows the filter to draw all but the last 2 % of the mobile phase from the reservoir without drawing air into the system. Compare this with conventional cylindrical mobile phase filters that begin to draw air into the system when less than 10 % of the solvent remains in the reservoir. The Solvent Saver Inlet Filter is manufactured by pressing a PEEK filter element into an inert Teflon housing. The top of the housing has female 1/4 in.-28 threads to accept 1/8 or 1/16 in. OD tubing via PEEK flangeless fittings and Tefzel ferrule (sold separately) or direct connect various size tubing using the Unifit adapter (sold separately). The Unifit adapter slip-fits onto 1.5, 2.2 or 3.0 mm ID tubing. This filter is excellent for sensitive biochromatography and ion chromatography applications where metal surfaces may corrode or interact with samples.

inleT FilTeRS

|

pump inlet FilterUni t adapter(sold separately AQ0-8339)

Ordering Information solvent inlet Filters - stainless steel Part No. Description AF0-0356 Solvent inlet Filter, 2 m, for 1/16 in. iD tubing AF0-0359 Solvent inlet Filter, 2 m, for 1/8 in. iD tubing At0-2955 Teflon Tubing, 5 ft. l x 1/8 in. oD x 1/16 in. (0.062 in.) iD At0-2956 Teflon Tubing, 10 ft. l x 1/8 in. oD x 1/16 in. (0.062 in). iD At0-8609 Teflon Tubing, 5 ft. l x 1/4 in. oD x 1/8 in. (0.125 in.) iD At0-8610 Teflon Tubing, 10 ft. l x 1/4 in. oD x 1/8 in. (0.125 in). iD

slip- ts onto 1.5, 2.2 or 3.0 mm ID tubing

1/4 in.-28 angeless tting (included) attaches to 1/8 in. OD tubing

Unit ea ea ea ea ea ea

Price

Important: Depending on the mobile phase, we recommend that you change your inlet filter every one to six months. For disposable Syringe Filters offering convenient and economical sample and solvent filtration, see pp. 10-12

Ordering Information solvent saver inlet Filter - Metal-Free Part No. Description Ah0-1562 Solvent Saver inlet Filter with 10 m peeK filter with Flangeless fitting for 1/8 in. oD tubing AQ0-8339 AQ0-2949 AQ0-2950 At0-2953 At0-2955 At0-2956 At0-8610 Solvent Saver Unifit Adapter, Tri-Step Tubing Connector, peeK Flangeless nut and Ferrule for 1/16 in. oD tubing, in.-28 threads, red Delrin Flangeless nut and Ferrule for 1/8 in. oD tubing, in.-28 threads, green Delrin Teflon Tubing, 5 ft. l x 1/16 in. oD x 1/32 in. (0.031 in.) iD Teflon Tubing, 5 ft. l x 1/8 in. oD x 1/16 in. (0.062 in.) iD Teflon Tubing, 10 ft. l x 1/8 in. oD x 1/16 in. (0.062 in). iD Teflon Tubing, 10 ft. l x 1/4 in. oD x 1/8 in. (0.125 in). iD

Unit ea ea 10/pk 10/pk ea ea ea ea

Price

16

Phenomenex

FiltrationIn-Line FiltersStainless Steel (Analytical) Removes particulates from flow path Minimizes sample peak dispersion Two column In-line Filters are available to protect expensive HPLC columns from damaging microparticulates. Using one of these filters between the injection valve and the column is recommended for all HPLC systems. The 3 mm diameter filter element is recommended for use with conventional 4.6 mm diameter columns. The 1.5 mm diameter filter is recommended for use with smaller diameter columns, microbore columns, and very short columns for fast HPLC procedures. The 1.5 mm version provides minimal dispersion for this type of filter, but has only about the sample load capacity of the 3 mm version. Both Column In-line Filters are supplied with two 6 cm L x 0.007 in. ID connecting tubes. Pressure rating of both models is 7000 psi.

best seller

KrudKatcher Universal fit to virtually all manufacturers analytical columns Saves expensive columns and equipment from damaging microparticulates Convenient, disposable in-line filter There are two types of KrudKatchers depending on system backpressure and dead volume demands: the KrudKatcher Classic and the KrudKatcher Ultra. Whereas conventional in-line filters typically cost much more and often require replacement parts and tools, the KrudKatcher is a simple, low-cost unit that is easily replaced and discarded when the backpressure indicates clogging or excessive particle build up. No replacement parts are required just unscrew, throw away and replace with a new filter in seconds. The universal connection is compatible with all standard 1/16 in. 10-32 internal threaded endfittings used in columns, guard columns, injector valves, and other LC equipment. FiLTrATiON | in-line FilTeRS

Ordering Information stainless steel in-Line Filters (Analytical) Part No. Description AF0-0375 in-line Filter with 0.5 m porosity x 1.5 mm dia. filter AF0-0376 Replacement Filter Disks: 0.5 m x 1.5 mm AF0-0377 in-line Filter with 0.5 m porosity x 3 mm dia. filter AF0-0378 Replacement Filter Disks: 0.5 m x 3 mm

Unit ea 5/pk ea 5/pk

Price

The KrudKatcher Classic: pressure-rated to 5,000 psi (345 bar) hand-tightened connection The PEEK filter body of the KrudKatcher Classic houses an integrated 0.5 m 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream with minimal contributions to system dead volume (2 L).Ordering Information KrudKatcher classic Disposable in-Line Filter Part No. Description AF0-5727 KrudKatcher Disposable pre-Column Filter, 0.5 m

Metal-Free/Biocompatible (Analytical) Biocompatible Effective pre-column filtration Virtually no band broadening or peak distortion Easy fingertight connection This in-line filter assembly removes fine particles from the solvent stream without adding band broadening or peak distortion to your separation. Microparticulates down to 0.5 m are effectively removed before they have a chance to plug your column and degrade your separation. The Polyglas frit with fluoropolymer frit assembly design is fully biocompatible and easily installed using fingertight connections.

Unit 10/pk

Price

The KrudKatcher Ultra: Fits virtually all UhplC / hplC columns 1.0 to 4.6 mm iD pressure rated to 20,000 psi (1,375 bar) extremely low dead volume minimizes sample peak dispersion The KrudKatcher Ultra filter body houses an integrated 0.5 m 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system backpressure or dead volume ( -0.5

Modified n-Vinylpyrrolidone functional group results in multiple mechanisms of retention for the widest range of selectivity. Strong retention of neutral compounds allows for aggressive washes for increased sensitivity and method reproducibility.

Acidic Analyte pKa 2

Strata-X-A Strata-XL-A

Dimethyl butyl quaternary amine functional group provides strong retention of weakly acidic analytes and allows for 100 % organic washes, increasing sensitivity and method reproducibility.

Strata-X-AW Strata-XL-AW

Acidic Analyte pKa 5

Di-amino functional group is highly selective for charged acidic compounds and allows for 100 % organic washes, increasing sensitivity and method reproducibility.

Strata-X-C Strata-XL-C

Basic Analyte pKa 10.5

Sulfonic acid functional group is highly selective for weakly basic compounds and allows for 100 % organic washes, increasing sensitivity and method reproducibility.

sAMPLe PrePArATiON

Basic Analyte pKa 8

Strata-X-CW Strata-XL-CWspecialty sorbents Application

Carboxylic acid functional group allows for reversible binding of strong bases and quaternary amines. high selectivity for all basic compounds allows for 100 % organic washes, increasing sensitivity and method reproducibility.Phase Description

Strata-X-Drug B

Basic Drugs of Abuse

proprietary strong cation-exchange sorbent that eliminates the need to condition / equilibrate the sorbent.

| STRATA-x polYMeRiC Spe

Strata-X-Drug N

Neutral Drugs of Abuse

proprietary reversed phase sorbent that eliminates the need to condition / equilibrate the sorbent.

Step 2. Select a Sorbent MassLoading capacity chartStrata-X PhasePlasma /serum Urine Filtered Tissue Homogenates Water (particulate free) Water (particulate-laden) Mass (mg in tube)

100 l 250 l

250 l 1 ml 2 ml 4 ml 8 ml 20 ml 125 l 500 l 1 ml 2 ml 4 ml 10 ml

10 mg 50 mg 100 mg 150 mg 300 mg 500 mg 5 mg 25 mg 50 mg 75 mg 150 mg 250 mg

n.A. n.R. n.R. 50 ml 100 ml 500 ml n.A. n.R. n.R. 25 ml 50 ml 250 ml

n.A. n.R. n.R. 25 ml 50 ml 100 ml n.A. n.R. n.R. 13 ml 25 ml 50 ml

10 mg 30 mg 60 mg 100 mg 200 mg 500 mg 10 mg 30 mg 60 mg 100 mg 200 mg 500 mg

strata-X, X-c, X-cW, X-A, X-AW

500 l 1 ml n.A. n.A. 50 l 125 l

strata-XL, XL-c, XL-cW, XL-A, XL-AW

250 l 500 l n.A. n.A.

N.A. = Not Applicable (not commonly used) N.R. = Not Recommended (may not provide expected results)

See the following pages for specific phase details and general extraction protocols.

Phenomenex

39

Strata -X Polymeric SPE

U.S. Patent No. 7,119,145

Strata-X and Strata-XLA reversed phase functionalized polymeric sorbent that gives strong retention of neutral, acidic, or basic compounds under aggressive, high organic wash conditions.strata-X, 33 m, 85 strata-XL, 100 m, 300

if Strata Spe products do not perform as well or better than your current Spe product of similar phase, mass and size, return the product with comparative data within 45 days for a FUll ReFUnD.

high Concentration Samples Small Target Analytes (< 10 kDa) large Target Analytes (> 10 kDa) large Volume Samples Viscous Samples

4 4 4 4 4

3 Mechanisms of Retention - Bonding

Hydrogen Bonding Dipole-Dipole Interactions

Hydrophobic Interaction

)

)

.. N

.. N

)

n

O

n

O

n .. N

O

)

)

STRATA-x polYMeRiC Spe | sAMPLe PrePArATiON

Strata-XOrdering Information sorbent Format Mass Tube 30 mg 30 mg 60 mg 100 mg 100 mg 200 mg 200 mg 500 mg 500 mg Giga Tube 500 mg 1g 1g 2g 5g Teflon Tube 200 mg 200 mg 96-Well Plate 10 mg 30 mg 60 mg 8e-S100-AgB 8e-S100-TgB 8e-S100-UgB 2 plates/Box 2 plates/Box 2 plates/Box Part Number 8B-S100-TAK 8B-S100-TBJ 8B-S100-UBJ 8B-S100-eBJ 8B-S100-eCh 8B-S100-FBJ 8B-S100-FCh 8B-S100-hBJ 8B-S100-hCh 8B-S100-hDg 8B-S100-JDg 8B-S100-Jeg 8B-S100-Keg 8B-S100-lFF 8B-S100-FBJ-T 8B-S100-FDg-T Unit 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 12 ml (20/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 3 ml (50/box) 12 ml (20/box) Price

Strata-XLOrdering Information sorbent Format Mass Tube 30 mg 60 mg 100 mg 200 mg 200 mg 500 mg Giga Tube 2g 2g 5g 5g 10 g 96-Well Plate 30 mg Part Number 8B-S043-TAK 8B-S043-UBJ 8B-S043-eBJ 8B-S043-FBJ 8B-S043-FCh 8B-S043-hCh 8B-S043-KDg 8B-S043-Keg 8B-S043-leg 8B-S043-lFF 8B-S043-MFF 8e-S043-TgB Unit 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 6 ml (30/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 60 ml (16/box) 2 plates/Box Price

* To control flow rate with Strata-XL, use a stopcock (AH0-6048) when processing samples with a vacuum manifold.

General Extraction ProtocolMethod written for a 30 mg/1 mL tube

On-line Extraction Cartridge Description Part Number Strata-x on-line extraction 00M-S033-B0-CB cartridge, 20 x 2.0 mm Cartridge holder, 20 mm Ch0-5845

conditionUnit/Box ea ea Price

1 mL Methanol followed by 1 mL D.I. Water

LoadRecommended volume of sample (see page 39)

WashFor Large Volume Cleanup/Flash Analysis, use Giga Tubes For SPE Vacuum Manifolds and Accessories, see pp. 57-59 The General Extraction Protocol is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.

1 mL 5-60 % Methanol in D.I. Water. Allow sorbent to dry for 30 seconds

elute2x 500 L 2 % Formic acid in Methanol or Acetonitrile

)

40

Phenomenex



Strata-X-C and Strata-XL-CA strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa less than 10.5, making 100 % organic wash conditions possible.strata-X-c, 33 m, 85 strata-XL-c, 100 m, 300

)

=


4 4

)

=

O O

)

S O - H44

4 +

=

=

if Strata Spe products do not perform as well or better than your current Spe product of similar phase, mass and size, return the product with comparative data within 45 days for a FUll ReFUnD. O

S O- H

+

O

)

)

3 Mechanisms of Retention Strong Cation-Exchange

- Bonding+


=

=

O O

O O

)

=

=

S O- H

S O- H

)

)

)

)

)

=

=

)

+

=

=

O O

S O- H

+

O O

S O- H

+

sAMPLe PrePArATiON |

Strata-X-C

Ordering Information O + Format sorbent Mass Part Number S O- H Tube 30 mg 8B-S029-TAK O 30 mg 8B-S029-TBJ 60 mg 8B-S029-UBJ 100 mg 8B-S029-eBJ 100 mg 8B-S029-eCh O 200 mg 8B-S029-FBJ + 200 mg -H S O8B-S029-FCh 500 mg 8B-S029-hBJ 500 mg 8B-S029-hCh O Giga Tube 500 mg 8B-S029-hDg 1g 8B-S029-JDg 1g 8B-S029-Jeg 2g 8B-S029-Keg 5g 8B-S029-lFF 96-Well Plate 10 mg 8e-S029-AgB 30 mg 8e-S029-TgB 60 mg 8e-S029-UgB

=

Unit 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 12 ml (20/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 2 plates/Box 2 plates/Box 2 plates/Box

)

Price

=

Strata-XL-C O

+ Ordering Information H sorbent Mass FormatO S Tube O 30 mg 60 mg 100 mg 100 mg 200 mg 200 mg 500 mg Giga Tube 2g 2g 5g 5g 10 g 96-Well Plate 30 mg

Part Number 8B-S044-TAK 8B-S044-UBJ 8B-S044-eBJ 8B-S044-eCh 8B-S044-FBJ 8B-S044-FCh 8B-S044-hCh 8B-S044-KDg 8B-S044-Keg 8B-S044-leg 8B-S044-lFF 8B-S044-MFF 8e-S044-TgB

Unit 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 6 ml (30/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 60 ml (16/box) 2 plates/Box

Price

)

=

)

)

=

STRATA-x polYMeRiC Spe

)

)

=

=

General Extraction ProtocolMethod written for a 30 mg/1 mL tubePrice

On-line Extraction Cartridge Description Part Number Strata-x-C on-line extraction 00M-S048-B0-CB cartridge, 20 x 2.0 mm Cartridge holder, 20 mm Ch0-5845

Unit/Box ea ea

condition1 mL Methanol followed by 1 mL D.I. Water

LoadRecommended volume of sample (pH 6-7) (see page 39)

Wash1 mL 0.1 N Hydrochloric acid in D.I. Water followed by 1 mL 0.1 N Hydrochloric acid in Methanol

elute2x 500 L 5 % Ammonium hydroxide in Methanol or AcetonitrileFor Large Volume Cleanup/Flash Analysis, use Giga Tubes For SPE Vacuum Manifolds and Accessories, see pp. 57-59 The General Extraction Protocol is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.

Phenomenex

41



Strata-X-CW and Strata-XL-CW

)

A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, including quaternary amines, making 100 % organic wash conditions possible.


O

)

strata-X-cW, 33 m, 85

strata-XL-cW, 100 m, 300


4 4 4 4 4

OH

)

O

)

O

3 Mechanisms of Retention Weak Cation-Exchange

)

- Bonding

OH

)


OH

)

)

OSTRATA-x polYMeRiC Spe | sAMPLe PrePArATiON

O

)

O

)

)

OH

OH

)

OH

Strata-X-CW

)

Ordering Information sorbent Format Mass Tube 30 mg 30 mg 60 mg 100 mg 200 mg 200 mg 500 mg 500 mg Giga Tube 500 mg 1g 1g 2g 5g 96-Well Plate 10 mg 30 mg 60 mg

OPart Number

Strata-XL-CW OPrice

)

OH

Unit

8B-S035-TAK 8B-S035-TBJ 8B-S035-UBJ 8B-S035-eCh O 8B-S035-FBJ 8B-S035-FCh OH 8B-S035-hBJ 8B-S035-hCh 8B-S035-hDg 8B-S035-JDg 8B-S035-Jeg 8B-S035-Keg 8B-S035-lFF 8e-S035-AgB 8e-S035-TgB 8e-S035-UgB

1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 12 ml (20/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 2 plates/Box 2 plates/Box 2 plates/Box

Ordering Information sorbent Format OH Mass Tube 30 mg 60 mg 100 mg 100 mg 200 mg 200 mg 500 mg Giga Tube 2g 2g 5g 5g 10 g 96-Well Plate 30 mg

)

)



Price

)

)

On-line Extraction Cartridge Description Part Number Strata-x-CW on-line extraction 00M-S036-B0-CB cartridge, 20 x 2.0 mm Cartridge holder, 20 mm Ch0-5845

General Extraction ProtocolUnit/Box ea ea Price

Method written for a 30 mg/1 mL tube




1 mL D.I. Water followed by 1 mL Methanol


42

Phenomenex



Strata-X-A and Strata-XL-AA strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with pKa greater than 2, making 100 % organic wash conditions possible.


)

CH3 N+ CH3

strata-X-A, 33 m, 85

strata-XL-A, 100 m, 300


4 4 4 4 4

)

CH3 N+ CH3

)

)

CH3 N+ CH Hydrophobic Interaction 3

)

3 Mechanisms of Retention Strong Anion-Exchange

- Bonding

)

)

CH3 N+ CH3

CH3 N+ CH3

)

)

CH3 N+ CH3sAMPLe PrePArATiON |

)

)

Strata-X-A

CH3 Ordering Information sorbent + Format Mass N Part Number Tube CH3 30 mg 8B-S123-TAK 30 mg 8B-S123-TBJ 60 mg 8B-S123-UBJ 100 mg 8B-S123-eBJ CH8B-S123-eCh 3 100 mg 200 mg N+ 8B-S123-FBJ 200 mg 8B-S123-FCh CH3 500 mg 8B-S123-hBJ 500 mg 8B-S123-hCh Giga Tube 500 mg 8B-S123-hDg 1g 8B-S123-JDg 1g 8B-S123-Jeg 2g 8B-S123-Keg 5g 8B-S123-lFF 96-Well Plate 10 mg 8e-S123-AgB 30 mg 8e-S123-TgB 60 mg 8e-S123-UgB

Unit

Price

1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 12 ml (20/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 2 plates/Box 2 plates/Box 2 plates/Box

3 Ordering Information + sorbent N Format Mass CHTube 3 30 mg 60 mg 100 mg 100 mg 200 mg 200 mg 500 mg Giga Tube 2g 2g 5g 5g 10 g 96-Well Plate 30 mg

Strata-XL-ACH

)

)

)

)Part Number 8B-S053-TAK 8B-S053-UBJ 8B-S053-eBJ 8B-S053-eCh 8B-S053-FBJ 8B-S053-FCh 8B-S053-hCh 8B-S053-KDg 8B-S053-Keg 8B-S053-leg 8B-S053-lFF 8B-S053-MFF 8e-S053-TgB


Price

)


)

)





1 mL 25 mM Ammonium acetate (pH 6-7) followed by 1 mL Methanol


Phenomenex

43



Strata-X-AW and Strata-XL-AW

)

A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with pKa less than 5, making 100 % organic wash conditions possible.


strata-X-AW, 33 m, 85

strata-XL-AW, 100 m, 300


4 4 4 4 4

)

3 Mechanisms of Retention Weak Anion-Exchange

)

NH

NH2

)

)

NH

NH2

- Bonding

)

)


)

)

Strata-X-AW

)

2 Ordering Information NH sorbent Format Mass Part Number Tube 30 mg 8B-S038-TAK 30 mg 8B-S038-TBJ 60 mg 8B-S038-UBJ 100 mg 8B-S038-eBJ 2 NH 100 mg NH8B-S038-eCh 200 mg 8B-S038-FBJ 200 mg 8B-S038-FCh 500 mg 8B-S038-hBJ 500 mg 8B-S038-hCh Giga Tube 500 mg 8B-S038-hDg 1g 8B-S038-JDg 1g 8B-S038-Jeg 2g 8B-S038-Keg 5g 8B-S038-lFF 96-Well Plate 10 mg 8e-S038-AgB 30 mg 8e-S038-TgB 60 mg 8e-S038-UgB

)

Unit 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 3 ml (50/box) 6 ml (30/box) 12 ml (20/box) 12 ml (20/box) 20 ml (20/box) 20 ml (20/box) 60 ml (16/box) 2 plates/Box 2 plates/Box 2 plates/Box

)

NH

Strata-XL-AW NH2Price

NH Ordering Information sorbent Format Mass Tube 30 mg 60 mg 100 mg 100 mg 200 mg 200 mg 500 mg Giga Tube 2g 2g 5g 5g 10 g 96-Well Plate 30 mg

)

NH

NH2

)

NH

NH2

)

)

NH

NH2


NH

NH2



Price

)

)



LoadRecommended volume of sample (pH 6-7) (see page 39)For Large Volume Cleanup/Flash Analysis, use Giga Tubes For SPE Vacuum Manifolds and Accessories, see pp. 57-59 The General Extraction Protocol is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.

Wash1 mL 25 mM Ammonium acetate (pH 6-7) followed by 1 mL Methanol

elute2x 500 L 5 % Ammonium hydroxide in Methanol or Acetonitrile

44

Phenomenex



Strata-X-Drug BA proprietary strong cation-exchange sorbent that is designed and quality controlled for basic drugs of abuse analysis. This sorbent does not require a conditioning/equilibrating step.


Strata-X-Drug B Certificate of Analysis

We test recovery by extracting codeine and morphine from urine to ensure that each batch provides you with the best results possible!


Strong cation-exchange sorbents can sometimes promote conversion of norcodeine and normorphine to parent compounds. Strata-X-Drug B is guaranteed not to promote this interconversion.


Strata-X-Drug B Extraction ProtocolsOrdering Information sorbent Format Mass Tube 10 mg 10 mg 30 mg 30 mg 30 mg 60 mg 60 mg 60 mg Giga Tube 100 mg 96-Well Plate 10 mg 30 mg 60 mgTab-less

1Part Number 8B-S128-AAK 8l-S128-AAK 8B-S128-TAK 8l-S128-TAK 8B-S128-TBJ 8B-S128-UBJ 8B-S128-UCh 8B-S128-UCl 8B-S128-eDg Unit 1 ml (100/box) 1 ml (100/box) 1 ml (100/box) 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 6 ml (200/box) 12 ml (20/box) Price Opiates, 6-MAM, PcP, Amphetamines, Methadone, Healthcare Opiates, and Propoxyphene* condition Wash 1 2 ml of 100 mM Sodium acetate buffer (ph 5.0) Wash 2 2 ml Methanol

2Marijuana Metabolites

3cocaine Metabolites

Not required 2 ml of 100 mM Sodium acetate buffer (ph 5.0) 2 ml of Acetonitrile:100 mM Sodium acetate buffer (ph 5.0) (30:70) 15 minutes under full vacuum 2 ml of ethyl acetate: isopropanol (85:15) 2 ml of 0.1 n hydrochloric acid 2 ml Methanol

Load pre-treated urine sample pre-treated urine sample pre-treated urine sample

8e-S128-AgB 8e-S128-TgB 8e-S128-UgB

2 plates/box 2 plates/box 2 plates/box

Dry 10 minutes under full vacuum elute 2 ml of ethyl acetate: isopropanol: Ammonium hydroxide (70:20:10)

10 minutes under full vacuum 2 ml of ethyl acetate: isopropanol: Ammonium hydroxide (70:20:10)

tube

* Opiates, 6-MAM, PCP, Amphetamines, Methadone, Healthcare Opiates, and Propoxyphene can be extracted simultaneously or separately using the same SPE methodology. Methods are written for 60 mg/6 mL Strata-X-Drug B; however they can be scaled to accommodate smaller or larger sample sizes and sorbent masses.

Phenomenex

45



Strata-X-Drug NA proprietary reversed phase sorbent that is designed and quality controlled for neutral drugs of abuse analysis. This sorbent does not require a conditioning/equilibrating step.


Strata-X-Drug N Certificate of Analysis

We test recovery by extracting lorazepam and temazepam from urine to ensure that each batch provides you with the best results possible!


Ordering Information sorbent Format Mass Tube 10 mg 10 mg 30 mg 30 mg 30 mg 60 mg 60 mg 60 mg 100 mg Giga Tube 100 mg 96-Well Plate 10 mg 30 mg 60 mgTab-less

Strata-X-Drug N Extraction ProtocolsPart Number 8B-S129-AAK 8l-S129-AAK 8B-S129-TAK 8l-S129-TAK 8B-S129-TBJ 8B-S129-UBJ 8B-S129-UCh 8B-S129-UCl 8B-S129-eCh 8B-S129-eDg Unit 1 ml (100/box) 1 ml (100/box) 1 ml (100/box) 1 ml (100/box) 3 ml (50/box) 3 ml (50/box) 6 ml (30/box) 6 ml (200/box) 6 ml (30/box) 12 ml (20/box) Price

1Barbiturates condition Load Wash 1 Wash 2 Dry elute pre-treated urine sample 2 ml of 0.1 n hydrochloric acid (hCi) 2x 2 ml of Methanol: 0.1 n hCi (30:70) 10 minutes under full vacuum 2 ml of ethyl acetate: isopropanol (85:15) Not required

2Benzodiazepines pre-treated urine sample 2 ml of Acetonitrile:Water (20:80) 10 minutes under full vacuum 2 ml of ethyl acetate: isopropanol (85:15)

8e-S129-AgB 8e-S129-TgB 8e-S129-UgB

2 plates/box 2 plates/box 2 plates/box

Methods are written for 100 mg/6 mL Strata-X-Drug N; however they can be scaled to accomodate smaller or larger sample sizes and sorbent masses.

tube

46

Phenomenex

Strata Traditional SPE

Strata Traditional Solid Phase Extraction (SPE) SorbentsMaterial CharacteristicsPhase Particle size (m) Pore size () surface Area (m2/g) carbon Load (%) Bonding end capping


ionic capacity (meq/g)

reversed PhaseC18-e C18-U C18-T C8 phenyl 55 55 55 55 55 55 55 55 55 55 55 200 55 55 55 170 200 120 100 proprietary proprietary proprietary 70 70 140 70 70 70 70 65 70 70 70 70 70 70 70 80 70 120 260 proprietary proprietary proprietary 500 500 300 500 500 500 500 550 500 500 500 500 500 500 500 300 proprietary 150 500 proprietary proprietary proprietary 18.0 17.0 15.0 10.5 10.5 10.0 5.0 0.0 9.0 8.0 6.5 proprietary proprietary proprietary 7.0 0.0 0.0 proprietary proprietary trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional trifunctional Yes no Yes Yes Yes no no no no no 1.3 0.9 0.8 0.9

Normal PhaseCn nh2 Silica (Si-1)

ion-exchangeSCx WCx SAx

Mixed-Mode


Screen-C gF Screen-C Screen-A ABW

specialtyFl-pR (Florisil) eph (extractable petroleum hydrocarbon) Al-n (Alumina-neutral) SDB-l eco-Screen Melamine pAh

STRATA TRADiTionAl Spe

Over 20 QA and QC Tests Ran on Strata SPE Products Surface Area Pore Size Pore Volume Surface pH Particle Diameter Particle Size Distribution % Water % SiO2 % Fe2O3 Purity Carbon Loading Hydrogen Loading Surface Coverage Residue Turbidity Sorbent Weight Flow Rate Tube Purity Frit Purity Dye Tests Chromatogram 11 Probes Used

Guaranteed Performance contaminate-free extractsPhenomenex Strata C18-E Waters Sep-Pak C18 Biotage IST ISOLUTE C-18(EC) Varian Bond Elut C18

Comparative separations may not be representative of all applications.

Phenomenex

47

Strata Traditional SPE

Determine the correct sorbent MassSilica-Based Sorbents (Strata C18-E, C8, SCX, SAX, WCX, NH2, etc.) sample MatrixBlood, serum, plasma Urine Filtered tissue homogenates


sorbent Mass50 mg sorbent per 250 l 50 mg sorbent per 500 l 100 mg sorbent per 100 mg tissue

environmental samplesWater (particulate-free) drinking Water (particulate-laden) rivers, runoff, etc. Soil extracts

sorbent Mass500 mg/100 ml - 500 ml sample 1 g/100 ml - 500 ml sample 1 g/100 g of soil extract

Determine the correct sorbent Wash and elution volumes10 mg silica-Based sorbent Mass 50 mg 100 mg 150 mg 200 mg 500 mg 1g 2g 5g 10 g

sAMPLe PrePArATiON

practical Minimum Wash and elution Volume 4 bed volumes Recommended Wash and elution Volume 8 bed volumes

60 l 300 l 120 l 600 l

600 l 1.2 ml

900 l 1.8 ml

1.2 ml 2.4 ml

3 ml 6 ml

6 ml

12 ml

30 ml 60 ml

60 ml 120 ml

12 ml 24 ml

General Extraction ProtocolStrata Reversed Phase Methodfor C18, C8, Phenyl, CN, SDB-L Sorbents

Strata Ion-Exchange Methodfor SCX, WCX, SAX, NH2 (WAX) Sorbents

Strata Normal Phase Methodfor Silica, Florisil, NH2, CN Sorbents

STRATA TRADiTionAl Spe

|

1 2 3 4 5

1ANALYTE PROPERTIES Low to moderate polarity (or non-polar), hydrophobic, neutralized/uncharged SAMPLE/MATRIX Aqueous, diluted with buffer CONDITIONING STEP 1. Solvation polar organic solvents 2. Equilibration aqueous, buffers WASH STEP Aqueous buffers with 5 to 50 % polar organic solvent ELUTION STEP Polar or non-polar organic solvent(s) with or without water, buffer and/or strong acid or base

ANALYTE PROPERTIES Ionized/charged compounds SAMPLE/MATRIX Aqueous; low ionic strength buffers (6 months) with the Microseal septum use the following injection port limits: (a) Agilent 6890, 5890 Series II - 325 C; (b) Agilent 5890A - 300 C. Higher temperatures will result in shorter lifetimes. (2) The Merlin Microseal Septum should only be used with a blunt-tipped, 0.63 mm diameter (0.025 in., 23 gauge) syringe needle (typically used with the Agilent 7673 autosampler). Sharp-pointed or sharp-edged needles should not be used as they may slice or pierce the seals.

Ordering Information Merlin Microseal septum Part No. Description AG0-5985 Merlin Microseal high pressure Septum Standard Kit, includes nut and 2 septa AG0-5986 Merlin Microseal high pressure Septum Starter Kit, includes nut and 1 septum replacement Parts AG0-5987 Merlin Microseal high pressure Septum AG0-5988 Merlin Microseal high pressure nut

Unit ea ea ea ea

Price

Syringes for Use With the Merlin MicrosealOrdering Information Part No. Mfr. No. Agilent P/N For Agilent 7673 Autosamplers As0-4386 87987 9301-0892 As0-4387 80387 9301-0713 As0-4388 80390 9301-0725 removable Needles As0-4401 7786-01 9301-0892 Description* 75ASn (23s/1.71in./hp) 701ASn (23s/1.71in./hp) 701ASn (23s/1.71in./hp) Rn (23s/1.71in./hp) capacity (L) 5 10 10 2.5-100 l Unit ea ea 6/pk 6/pk Price

NOTE: Replacement needles are available in packs of three. * Values in parentheses denote the following: (gauge/length/point style). s after gauge denotes smaller/reduced internal needle diameter.

Contact Phenomenex or your local Phenomenex distributor for additional GC products and applications.

Phenomenex

113

GC AccessoriesFerrule Remover Tool Kit Simple and convenient tools effectively remove stuck ferrules from fittings Special spiral-cut rachet design slides in and grabs ferrule tight Includes two tools for removing capillary ferrules from 0.4 to 0.8 mm ID

Ceramic Scoring Wafers High quality ceramic cutting tool for fused silica capillary columns

Ordering Information Ferrule remover Tool Kit Part No. Description Ad0-4725 gC Ferrule Remover Tool Kit

Unit ea

Price

Ordering Information ceramic scoring Wafers Part No. Description AG0-4718 Ceramic Scoring Wafer

Unit 2/pk

Price

CeRAMiC SCoRing WAFeRS / Tool & CleAning KiTS | Gc cOLUMNs & AccessOries

Flame Detector Jet Cleaning Kit For routine maintenance of Flame Ionization Detectors Includes: - Set of 3 jet reamers 0.008, 0.08 and 0.02 in. - 1 Stainless steel and 1 brass brush - 1 Dual-ended pin vise for holding reamers and brushes Use after the flame jet has been taken apart or while installed in the GC

Injection Port Cleaning Kit Includes 4 stainless steel brush sizes 1/8, 3/16, 1/4 and 3/8 in. Also includes a scraper-pick combo for hard septum residues and septum removal Easy to use with twisted wire handles and a convenient wire ring to keep parts together

Ordering Information Flame Detector Jet cleaning Kit Part No. Description Ad0-4723 gC Flame Detector Jet Cleaning Kit

Unit ea

Price

Ordering Information injection Port cleaning Kit Part No. Description Ad0-4724 gC injection port Cleaning Kit

Unit ea

Price

Contact Phenomenex or your local Phenomenex distributor for additional GC products and applications.

114

Phenomenex

Zebron GC Column Applications

ENVIRONMENTALSemi-volatile Organics: EPA Method 8270Ccolumn: Dimensions: Part No.: injection: carrier Gas: Oven Program: Zebron ZB-5 with 5 meter guardian 30 meter x 0.25 mm x 0.25 m 7hg-g002-11-ggA Split 10:1 @ 250 C, 1 l helium @ 0.9 ml/ min (constant flow) 50 C for 2 min to 70 C @ 20 C/min to 195 C @ 16 C/min to 285 C @ 30 C/min to 320 C @ 6 C/min for 4 min Detector: MSD; 30-450 amu

3

5

7

9

11

13

15

17

19

21 min

18-20 36,37 21-22 35 29 32 34 13 15 16 23 39 12 27 31 33 14 38 9 11 17 2425 26 28 8 10 30 6 7 8 9 min

1 2 3 4

3

4

5 5

6

7

App iD 14945

Gc cOLUMNs & AccessOries

69,70 56,57 59 44 45 51 54,55 46 48 53 47 49 50 52 62 74

84,85 83 86 103,104 90 92 89 87 88 13 14 91 95 94 93 96 98 97 102 105 101 100 99 107 106 110,111 112 109

40,41 4243

80 66,67 77,78 79 68 75 82 63 81 72 64 71 65 73 58 61 76 60 11.5 12.5 min

108

8.5

9.5

10.5

15

16

17

18

19

20

21 min

| ZeBRon gC ColUMn AppliCATionS

Peak No. sample Analyte1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. n-nitrosodimethylamine pyridine 2-picoline n-nitrosomethylethylamine Methyl Methanesulfonate n-nitrosodiethylamine ethyl Methanesulfonate phenol Aniline Bis(2-chloroethyl)ether 2-Chlorophenol 1,3-Dichlorobenzene 1,4-Dichlorobenzene Benzyl Alcohol 1,2-Dichlorobenzene 2-Methylphenol Bis(chloroisopropyl)ether 4-Methylphenol; 3-Methylphenol n-nitrosopyrrolidine Acetophenone n-nitrosomorpholine n-nitroso-di-n-propylamine 2-Toluidine hexachloroethane nitrobenzene n-nitrosopiperidine isophorone 2-nitrophenol 2,4-Dimethylphenol

Peak No. sample Analyte30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. Benzoic Acid Bis(2-chloroethoxy)methane 2,4-Dichlorophenol a,a-Dimethylphenylamine 1,2,4-Trichlorobenzene naphthalene 4-Chloroaniline 2,6-Dichlorophenol hexachloropropene hexachloro-1,3-Butadiene 1,4-phenylenediamine n-nitrosodibutylamine 4-Chloro-3-methylphenol Safrole 2-Methylnaphthalene 1-Methylnaphthalene 1,2,4,5-Tetrachlorobenzene hexachlorocyclopentadiene 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol isosafrole (isomer) 2-Chloronaphthalene 2-nitroaniline 1,4-naphthoquinone Dimethyl phthalate 1,3-Dinitrobenzene 2,6-Dinitrotoluene Acenaphthylene 3-nitroaniline

Peak No. sample Analyte59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. Acenaphthene 2,4-Dinitrophenol 4-nitrophenol Dibenzofuran pentachlorobenzene 2,4-Dinitrotoluene 1-naphthalenamine 2-naphthalenamine 2,3,4,6-Tetrachlorophenol Diethyl phthalate Fluorene 4-Chlorophenyl phenyl ether 2-Methyl-5-nitroaniline 4-nitroaniline 2-Methyl-4,6-dinitrophenol Diphenylamine Azobenzene 1,3,5-Trinitrobenzene phenacetin 4-Bromophenyl phenyl ether hexachlorobenzene 4-Aminobiphenyl pentachlorophenol pentachloronitrobenzene phenanthrene Dinoseb Anthracene Di-n-butyl phthalate 4-nitroquinoline-n-oxide

Peak No. sample Analyte88. 89. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105. 106. 107. 108. 109. 110. 111. 112. Methapyrilene isodrin Fluoranthene Benzidine pyrene Aramite (isomer) Aramite (isomer) Dimethylaminoazobenzene Chlorobenzilate 3,3-Dimethylbenzidine Benzyl butyl phthalate Kepone 2-Acetylaminofluorene 3,3'-Dichlorobenzidine Benz[a]anthracene Bis(2-ethylhexyl) phthalate Chrysene Di-n-octyl phthalate Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene 3-Methylcholanthrene indeno[1,2,3-cd]pyrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene

Phenomenex

115

6

128

2

123

Zebron GC Column Applications3

15696

1

5

4

7

8

133 131 129 130 132

124

15696

134 135

136 137

ENVIRONMENTAL15696Semi-volatile Organics: EPA Method 8270column: Dimensions: Part No.: injection: carrier Gas: Oven Program:15 21-25 29

2

3

4

5

6

7

8

9

10 125-127

11

12105-123See inset

13

14 125-127

15

16

min114,115

41 58

125-127

Zebron ZB-5ms 20 meter x 0.18 mm x 0.32 m 7FD-g010-51 16 26,27 9 Splitless @ 280 C, 1 l 10 helium @ 1.2 ml/min (constant flow) 14 17 18 19 11 45 C6to 200 C @ 20 13 12 C/min to 300 C 28 20 @ 25 C/min for 5.5 min Detector: MSD; 45-450 amu sample: All analytes are at 15 ppm, all 2 2 internal standards (iS) and 4 surrogates (surr) are 40 ppm.

100-102 42 43,44 45 46 49 50 47 485 min 6

68 116 117

30

631

34 35,36 39,40 32 37 38 33

80-82 51 52 53,54 56 57 557

60 59

128 6663 65 61 62 67 64

69 73,74

96-98 99

6

123

91 85 92,93 79 86 8789,90 94 72 75 76 78 83 71 77 95 84 88 708 133

104 128

103

111 113 106 107 105 110 112

121 118 120 122 123 119

128 1239

108 10910

124

min

124

133

11

min

2

1

5 4

7

8

1

5 4 3

7

8

134

135

2

3

4

5

61

22

5 4

38 773

8

449-46See inset

9

55

6 10641

7 117

8 128

9 13958

10 141011

11 1512

12 16

13 min

14

15 136 16 137135

App iD 15696

3

3

131 129 130 132

131 123 130 132 124 133 134 136 131 129 137 135 130 132 134 129

136 137

min114,115

13

114,115

14

15

16

min114,115

41

47-104See inset

58 21-25 15 29 42 43,4421-25 1521-25 15 16 29

29

41 58

100-102


42 43,44 68

100-102 68116 117

9 10 14 11 13 12

16 17 18 19 20

26,27

4

100-102 80-82 116 111 113 68 80-82 34 35,36 39,4042 45 96-98 103 69 117 30 43,44 46 34 35,36 39,40 10 45 14 17 18 19 96-98 73,74 91121 85 32 37 38 104 92,93 11 51 52 30 69 73,74 66 13 106 91 60 118 85 32 37 38 79 12 46 86 8789,90 92,93 16 51 52 28 66 64 53,54 122 26,27 9 33 60 50 120 94 103 99 72 7580-82 110 49 79 63 76 78 83 86 8789,90 59 107 28 20 50 53,54 34 35,36 39,40 64 45 96-98 123 104 94 62 99 65 33 71 77 72 47 78 83 56 57 49 63 10 61 30 31 57 59 6967 75 76 95 14 17 18 19 73,74 105 46 85 84 91 92,93 32 37 38 62 65 71 77 51 52 56 11 88 47 61 66 31 70 108 112 67 95 13 84 48 60 79 55 88 12 86 87 28 109 89,90 94 119 99 70 64 53,54 48 72 75 76 78 83 49 50 63 59 55 33 20 65 71 77 56 57 6 7 8 9 4 5 min 47 61 62 31 67 95 84 min 6 7 8 9 10 11 min 5 min 88 70 48 55

9

26,27

103 104

111 113 106

105 min

121 118 110 120 122 107 116 111 113 123 117 105 121 108 112 106 118 109 122 119 110 120 107 123108 10910

10 11211911

11

min

Peak No. sample Analyte1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. n-nitrosodimethylamine pyridine 2-Methylpyridine n-Methyl-n-nitrosoethanamine Methyl Methanesulfonate 2-Fluorophenol (surr) n-nitrosodiethylamine ethyl Methanesulfonate phenol-d6 (surr) phenol Aniline Bis(2-Chloroethyl)ether 2-Chlorophenol 1,3-Dichlorobenzene 1,4-Dichlorobenzene-d4 (iS) 1,4-Dichlorobenzene Benzoyl Alcohol 1,2-Dichlorobenzene 2-Methylphenol Bis(2-Chloroisopropyl)ether 4-Methylphenol 3-Methylphenol n-nitrosopyrrolidine Acetophenone n-nitroso-di-n-propylamine n-nitrosomorpholine o-Toluidine hexachloroethane nitrobenzene-d5 (surr) nitrobenzene n-nitrosopiperidine isophorone 2-nitrophenol 2,4-Dimethylphenol Triethylphosphorothioate

Peak No. sample Analyte36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70.

4

5

min

6

Peak No. sample Analyte71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. 88. 89. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105.

7

8

Peak No. sample Analyte106. 107. 108. 109. 110. 111. 112. 113. 114. 115. 116. 117. 118. 119. 120. 121. 122. 123. 124. 125. 126. 127. 128. 129. 130. 131. 132. 133. 134. 135. 136. 137.

9

min

min

Benzoic Acid Bis(2-Chloroethoxy)methane 2,4-Dichlorophenol 1,2,4-Trichlorobenzene phentermine naphthalene-d8 (iS) naphthalene 4-Chloroaniline 2,6-Dichlorophenol hexachloropropene hexachlorobutadiene n-nitrosodibutylamine 1,4-Diphenylenediamine 4-Chloro-3-methylphenol isosafrole 2-Methylnaphthalene 1-Methylnaphthalene hexachlorocyclopentadiene 1,2,4,5-Tetrachlorobenzene Safrole (isomer) 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2-Fluorobiphenyl (surr) Safrole 2-Chloronaphthalene 2-nitroaniline 1,4-naphthoquinone Dimethyl phthalate 1,3-Dinitrobenzene 2,6-Dinitrotoluene Acenaphthylene 3-nitroaniline Acenaphthene-d10 (iS) Acenaphthene 2,4-Dinitrophenol

4-nitrophenol pentachlorobenzene 2,4-Dinitrotoluene Dibenzofuran 1-naphthylamine Tetrachlorophenol 2-naphthylamine Diethyl phthalate Zinophos (Thionazin) Fluorene 4-Chlorophenylphenylether 2-Methyl-5-nitroaniline 4-nitroaniline 4,6-Dinitro-2-Methylphenol Diphenylamine Azobenzene 2,4,6-Tribromophenol (surr) 1,3,5-Trinitrobenzene Diallate phorate phenacetin 4-Bromophenylphenylether Diallate (isomer) hexachlorobenzene Dimethoate pentachlorophenol pentachloronitrobenzene 4-Aminobiphenyl pronamide phenanthrene-d10 (surr) Dinoseb Disulfoton phenanthrene Anthracene Methyl parathion

Di-n-butyl phthalate ethyl parathion 4-nitroquinoline-n-oxide Methapyrilene isodrin Fluoranthene Benzidine pyrene Aramite p-Terphenyl-d14 (surr) Aramite (isomer) Dimethylaminoazobenzene Chlorobenzilate Famfur 3,3'-Dimethylbenzidine Benzyl butyl phthalate Kepone 2-Acetylaminofluorene 3,3'-Dichlorobenzidine Benz[a]anthracene Chrysene-d12 (iS) Bis(2-ethylhexyl) phthalate Chrysene Di-n-octylphthalate Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene perylene-d12 (iS) 3-Methylcholanthrene indeno[1,2,3-cd]pyrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene

ZeBRon gC ColUMn AppliCATionS

|

116

Phenomenex

Zebron GC Column Applications

ENVIRONMENTALPolynuclear Aromatic Hydrocarbons: EPA Method 8100column: Dimensions: Part No.: injection: carrier Gas: Oven Program: Detector: sample: Zebron ZB-5ms 10 meter x 0.10 mm x 0.10 m 7CB-g010-02 Split 20:1 @ 320 C, 1 l helium @ 0.6 ml/min (constant flow) 100 C to 200 C @ 33 C/min to 320 C @ 25 C/min FiD @ 330 C 1. naphthalene 10. Benz[a]anthracene 2. 2-Methylnaphthalene 11. Chrysene 3. Acenaphthalene 12. Benzo[b]fluoranthene 4. Acenaphthene 13. Benzo[k[fluoranthene 5. Fluorene 14. Benzo[a]pyrene 6. phenanthrene 15. indeno[1,2,3-cd]pyrene 7. Anthracene 16. Dibenz[a,h]anthracene 8. Fluoranthene 17. Benzo[g,h,i]perylene 9. pyrene

Polynuclear Aromatic Hydrocarbonscolumn: Dimensions: Part No.: injection: carrier Gas: Oven Program: Zebron ZB-5ms 30 meter x 0.25 mm x 0.25 m 7hg-g010-11 Split 15:1 @ 310 C, 1 l helium @ 1.4 ml/min (constant flow) 170 C to 240 C @ 15 C/min to 275 C @ 4 C/min to 320 C @ 10 C/min for 5 min Detector: FiD @ 330 C sample: 1. naphthalene 10. Benz[a]anthracene 2. 2-Methylnaphthalene 11. Chrysene 3. Acenaphthalene 12. Benzo[b]fluoranthene 4. Acenaphthene 13. Benzo[k[fluoranthene 5. Fluorene 14. Benzo[a]pyrene 6. phenanthrene 15. indeno[1,2,3-cd]pyrene 7. Anthracene 16. Dibenz[a,h]anthracene 8. Fluoranthene 17. Benzo[g,h,i]perylene 9. pyrene6 7 8

5 44 3 5 6 7 8 9 10 12 2 1 11 13 14 15 16 17

3 2 1

9

11 10 13 14 12

16 15 17


App iD 15806

2

3

4

5

6

7

min

2

4

6

8

10

12

14

16

18

min

PAHs: US and EU Compoundscolumn: Dimensions: Part No.: injection: carrier Gas: Oven Program: Detector: sample: Zebron ZB-35 30 meter x 0.25 mm x 0.25 m 7hK-g003-11 on-Column @ 83 C, 1 l helium @ 1.2 ml/min (constant flow) 80 C for 0.66 min to 250 C @ 20 C/min to 360 C @ 8 C/min for 6 min MSD @ 360 C; 45-450 amu All analytes are 10 ppm in dichloromethane 1. naphthalene 14. Benzo[j]fluoranthene 2. 2-Methylnaphthalene 15. Benzo[a]pyrene 3. Acenaphthylene 16. 3-Methylcholanthrene 4. Acenaphthene 17. Dibenz[a,h]acridine 5. Fluorene 18. Dibenz[a,j]acridine 6. phenanthrene 19. indeno[1,2,3-cd]pyrene 7. Anthracene 20. Dibenz[a,h]anthracene 8. Fluoranthene 21. Benzo[g,h,i]perylene 9. pyrene 22. 7h-dibenzo[c,g]carbazole 10. Benz[a]anthracene 23. Dibenzo[a,e]pyrene 11. Chrysene 24. Dibenzo[a,i]pyrene 12. Benzo[b]fluoranthene 25. Dibenzo[a,h]pyrene 13. Benzo[k]fluoranthene

Polybrominated Diphenyl Ethers (PBDEs)column: Dimensions: Part No.: injection: carrier Gas: Oven Program: Detector: sample: Zebron ZB-5hT inferno 15 meter x 0.25 mm x 0.10 m 7eg-g015-02 on-Column @ 73 C, 0.5 l helium @ 1.5 ml/min (constant flow) 70 C to 160 C at 25 C/min to 350 C at 10 C/min for 5 min eCD @ 400 C Sample was 2.5 ppm in isooctane 1. BDe-25 11. BDe-99 2. BDe-28 12. BDe-85 3. BDe-75 13. BDe-154 4. BDe-49 14. BDe-153 5. BDe-71 15. BDe-138 6. BDe-47 16. BDe-183 7. BDe-66 17. BDe-190 8. BDe-77 18. BDe-203 9. BDe-100 19. BDe-209 10. BDe-119

App iD 16017

| ZeBRon gC ColUMn AppliCATionS

16144 6

18525 192 4 3 5 7

8

14 11 910 12 13 15 17 16 18

App iD 18525

21 3 4 5 6 7 8

1

1

2

9

Catalog Phenomenex 2012

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Transcript of Catalog Phenomenex 2012