CAMA-1MDA-MB-361 (HER2+)HCC-1569 (HER2+)BT474 (HER2+)HS578TMDA-MB-415...
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Transcript of CAMA-1MDA-MB-361 (HER2+)HCC-1569 (HER2+)BT474 (HER2+)HS578TMDA-MB-415...

CA
MA
-1
MD
A-M
B-3
61
(HE
R2
+)
HC
C-1
56
9 (H
ER
2+
)
BT
47
4 (H
ER
2+
)
HS
57
8T
MD
A-M
B-4
15
(Lu
min
al)
MD
A-M
B-2
31
MD
A-M
B-4
68
MD
A-M
B-4
36
BT
20
T
MD
A-M
B-1
57
76
N
10
A
76
NF
2V
76
NE
7
T4
7D
ZR
75
-1
MC
F-7
MD
A-M
B-4
53
(HE
R2
+)
HC
C3
8
M4
A4
NM
2C
5
HC
C-1
50
0
HC
C-1
95
4
HC
C-1
42
8
HC
C-1
41
9 (H
ER
2+
)
HC
C-1
93
7
DU
44
75
HC
C-1
80
6
UA
CC
-89
3 (H
ER
2+
)
AU
56
5 (H
ER
2+
)
SK
BR
3 (H
ER
2+
)
UA
CC
89
3 (H
ER
2+
)
BT
48
3
BT
54
9
Cy
cli
n E
Actin
Cy
cli
n E
Actin
Cy
cli
n E
Actin
De
ns
ito
me
try
Un
its
Subtype
LMW-E Levels
A
B
p=
0.0
03
Supplemental Figure 1
Full Length Cyclin E Levels

Protein FC:Sen p-value
I3C SensitiveCaspase 3 (active) 1.7 3.31E-08
p53 1.6 1.77E-10S6 pS235/pS236 1.3 0.02
p21 1.3 0.000111AMPK pT172 1.3 0.00043
Smac -1.2 0.0164E-BP1 pS65 -1.2 6.38E-09
PRAS40 pT246 -1.2 0.000488eEF2K -1.3 2.52E-06B-Raf -1.3 <1.0E-15
Paxillin -1.4 5.97E-1053BP1 -1.4 1.33E-11c.Myc -1.4 4.70E-08IAP -1.5 3.24E-11
HER3 -1.6 <1.0E-15Rb pS807/S811 -1.7 <1.0E-15
AIB1(SRC-1) -1.7 4.33E-15AR -1.8 <1.0E-15
GATA3 -2.0 1.53E-08PR -3.0 <1.0E-15
ER alpha -6.3 <1.0E-15I3C Insensitive FC:InSen p-value
Src pY416 1.3 0.000467Src pY527 1.3 2.35E-09
STAT3 pY705 -1.2 1.00E-05Caspase 7 cleaved D198 -1.4 0.00317
PKC alpha -3.0 2.72E-10Both FC:Sen p-value FC:InSen p-valueSnail 2.0 3.11E-12 1.6 4.63E-06c.Jun 1.4 3.83E-07 1.2 0.000817
Cyclin E1 1.4 2.21E-13 1.3 2.22E-12GSK3 alpha pS21/beta pS9 1.3 7.73E-09 1.3 3.04E-13
IGF-1R beta -1.4 <1.0E-15 -1.4 <1.0E-15Fibronectin -1.7 0.000102 -1.4 0.0166
Supplemental Table 1

I3C Sensitive Cells p-valueNRF2-mediated Oxidative Stress Response 2.00E-10Cell Cycle: G2/M DNA Damage Checkpoint Regulation 2.14E-08Protein Ubiquitination Pathway 1.07E-07Role of BRCA1 in DNA Damage Response 3.55E-07Molecular Mechanisms of Cancer 1.20E-06PI3K/AKT Signaling 1.45E-06Role of CHK Proteins in Cell Cycle Checkpoint Control 1.78E-06p53 Signaling 2.40E-06Actin Nucleation by ARP-WASP Complex 5.75E-06Mitotic Roles of Polo-Like Kinase 1.00E-05ATM Signaling 1.00E-05Purine Metabolism 1.20E-05Aminoacyl-tRNA Biosynthesis 3.31E-05Breast Cancer Regulation by Stathmin1 3.55E-05Hereditary Breast Cancer Signaling 3.63E-05Fcγ Receptor-mediated Phagocytosis in Macrophages and Monocytes 3.98E-05Apoptosis Signaling 5.50E-05Inositol Phosphate Metabolism 6.03E-05Glioma Signaling 7.94E-05Rac Signaling 9.55E-05Pancreatic Adenocarcinoma Signaling 1.12E-04Aryl Hydrocarbon Receptor Signaling 1.23E-04Induction of Apoptosis by HIV1 1.29E-04Estrogen Receptor Signaling 1.35E-04Cell Cycle Control of Chromosomal Replication 1.78E-04
I3C Insensitive Cells p-valueNRF2-mediated Oxidative Stress Response 7.76E-0714-3-3-mediated Signaling 1.15E-05Valine, Leucine and Isoleucine Degradation 1.51E-05Integrin Signaling 2.95E-05Fcγ Receptor-mediated Phagocytosis in Macrophages and Monocytes 6.03E-05IL-8 Signaling 7.76E-05VEGF Signaling 9.77E-05One Carbon Pool by Folate 1.58E-04Virus Entry via Endocytic Pathways 1.95E-04Rac Signaling 2.24E-04Axonal Guidance Signaling 2.75E-04Actin Nucleation by ARP-WASP Complex 2.82E-04Role of Tissue Factor in Cancer 3.16E-04HER-2 Signaling in Breast Cancer 3.16E-04Actin Cytoskeleton Signaling 3.47E-04Signaling by Rho Family GTPases 3.55E-04Thrombin Signaling 4.17E-04Docosahexaenoic Acid (DHA) Signaling 4.57E-04Breast Cancer Regulation by Stathmin1 5.50E-04Glioma Signaling 5.50E-04Mechanisms of Viral Exit from Host Cells 6.46E-04Cell Cycle: G2/M DNA Damage Checkpoint Regulation 7.08E-04PI3K/AKT Signaling 7.41E-04Macropinocytosis Signaling 8.32E-04Germ Cell-Sertoli Cell Junction Signaling 1.07E-03
Supplemental Table 2

Supplemental Figure 2
IC50 (μM)
Control 490.8shAHR.1 585.0shAHR.2 570.0
MDA-MB-157
B
ControlshAHR.1shAHR.2
Actin
AhR
Control sh-AHR.1 sh-AHR.2
No
rma
lize
d A
bs
orb
an
ce
(5
90
nM
)
Log I3C concentration (μM)
D 100 250 D 100 250 D 100 250 μM
A

No
rma
lize
d A
bs
orb
an
ce
(5
90
nM
)
Log Tamoxifen concentration (μM)
A
No
rma
lize
d A
bs
orb
an
ce
(5
90
nM
)Log I3C concentration (μM)
B
ParentalTAM-R
IC50 (μM)
Parental 0.9TAM-R 5.2
IC50 (μM)
Parental 277.9TAM-R 253.1
Supplemental Figure 3

T47D
ZR75-1
MCF-7
MDA-MB-436
MDA-MB-231
MDA-MB-157
I3C (μM)
p53
Actin
- + - + - + - + I3C (250 μM)
Control sh1 sh2 sh3
MCF-7A B
p53
IC50 (μM)
Control 265.2shP53.1 254.2shP53.2 243.8shP53.3 222.3
C
MCF-7
Supplemental Figure 4N
orm
ali
zed
Ab
so
rba
nc
e (
59
0 n
M)
Log I3C concentration (μM)
ControlshP53.1shP53.2shP53.3
p53 shRNA