calicut Digree Syllabus And Scheme For Biotechnology

8/6/2019 calicut Digree Syllabus And Scheme For Biotechnology

1/129

UNIVERSITY OF CALICUT

B.TECH BIOTECHNOLOGY

CURRICULUM

And

SYLLABUS

(3RD SEMESTER 8TH SEMESTER)


2/129

B. TECH BIOTECHNOLOOGY

THIRD SEMESTERCode Subject Hours/week Marks

L T P/D Internal Semester-

end

Semester-end

Examination

duration

hours

Credits

EN 09 301 Engineering Mathematics III 3 1 - 30 70 3 4

EN 09 302 Humanities &Communication

skills

2 1 - 30 70 3 3

BT 09 303 Fluid flow operations 4 1 - 30 70 3 5

BT 09 304 Microbiology 3 1 - 30 70 3 4

BT 09 305 Biochemistry I 3 1 - 30 70 3 4

BT 09 306 Bioprocess calculations 3 1 - 30 70 3 4

BT 09 307 (P) Microbiology Lab 3 50 50 3 2

BT 09 308 (P) Biochemistry Lab 3 50 50 3 2

Total 18 6 6 28

B. TECH. BIOTECHNOLOOGY

FOURTH SEMESTER

Code Subject Hours/week Marks


end

Semester-end

Examination duration

hours

Credits

EN 09 401 Engineering Mathematics IV 3 1 - 30 70 3 4

EN 09 402 Environmental Science 2 1 - 30 70 3 3

BT 09 403 Chemical Reaction Engg. 4 1 - 30 70 3 5

BT 09 404 Biochemistry II 3 1 - 30 70 3 4

BT 09 405 Cell Biology 3 1 - 30 70 3 4

BT 09 406 Mechanical Operations 3 1 - 30 70 3 4

BT 09 407 (P) Fluid Flow and Mechanical

Operations lab

- - 3 50 50 3 2

BT 09 408 (P) Bioanalytical techniques Lab 3 50 50 3 2

Total 18 6 6 28


FIFTH SEMESTER


3/129



end

Semester-end


hours

Credits

BT 09 501 Mass Transfer Operations 4 1 - 30 70 3 5

BT 09 502 Molecular Biology 3 1 - 30 70 3 4

BT 09 503 Enzyme Science and Engg 3 1 - 30 70 3 4

BT 09 504 Thermodynamics & Heat

Transfer Operations

3 1 - 30 70 3 4

BT 09 505 Bioinformatics 3 1 - 30 70 3 4

BT 09 506 Process Instrumentation 2 1 - 30 70 3 3

BT 09 507 (P) Heat and Mass Transfer Lab - - 3 50 50 3 2

BT 09 508 (P) Bioprocess Engg. Lab - - 3 50 50 3 2

Total 18 6 6 28


SIXTH SEMESTER



end

Semester-end


hours

Credits

BT 09 601 Genetic Engineering 4 1 - 30 70 3 5

BT 09 602 Bioprocess Engineering. 3 1 - 30 70 3 4

BT 09 603 Financial Management &

Cost Estimation of Process

Industries

3 1 - 30 70 3 4

BT 09 604 Process dynamics & control 3 1 - 30 70 3 4

BT 09 605 Food Biotechnology 2 1 - 30 70 3 3

BT 09 LXX Elective I 3 1 - 30 70 3 4

BT 09 607 (P) Molecular Biology & Genetic

Engg. Lab

- - 3 50 50 3 2

BT 09 608 (P) Bioinformatics Lab - - 3 50 50 3 2

Total 18 6 6 28


SEVENTH SEMESTERCode Subject Hours/week Marks


end

Semester-end


hours

Credits

BT 09 701 Bioprocess Plant Design 4 1 - 30 70 3 5

BT 09 702 Downstream Processing 3 1 - 30 70 3 4

BT 09 703 Environmental Engineering 2 1 - 30 70 3 3

BT 09 704 Immunology & Immuno

-technology

2 1 - 30 70 3 3

Elective I

1 BT 09 L 01 Plant Biotechnology2 BT 09 L 02 Animal Biotechnology3 BT 09 L03 Protein Engineering4 BT 09 L 04 Metabolic Engineering5 BT 09 L 05 Genomics & Proteomics


4/129

BT 09LXX Elective II 3 1 - 30 70 3 4

BT 09 LXX Elective III 3 1 - 30 70 3 4

BT 09 707 (P) Downstream processing lab - - 3 50 50 3 2

BT 09 708 (P) Reaction Engg& Process

Control Lab

- - 3 50 50 3 2

BT 09 709 (P) Project - - 1 50 1

Total 17 6 7 28


EIGHTH SEMESTER



end

Semester-end


hours

Credits

BT 09 801 Plant operations, safety and

biosafety

4 1 - 30 70 3 5

BT 09 802 Industrial Biotechnology and

Biopharmaceuticals

3 1 - 30 70 3 3

BT 09 LXX Elective IV 3 1 - 30 70 3 4BT 09 LXX Elective V 2 1 - 30 70 3 4

BT 09 805(P) Seminar - - 3 100 3 2

BT 09 806(P) Project - - 11 100 - - 7

BT 09 807 Viva-voce - - - - 100 - 3

Total 12 4 14 28

ELECTIVES

For ELECTIVE II

ELECTIVE III

ELECTIVE IV

ELECTIVE V

1 BT 09 L06 Gene and stem cell therapy

2 BT 09 L07 Molecular diagnostics

3 BT 09 L08 Molecular pathogenesis

4 BT 09 L09 Membrane separation technology

5 BT 09 L10 Recombinant DNA technology

6 BT 09 L11 DNA microarray technology

7 BT 09 L12 Cancer biology

8 BT 09 L13 Structural biology

9 BT 09 L14 Molecular modelling and drug design

10 BT 09 L15 Biosensors and bioinstrumentation

11 BT 09 L16 Molecualar medicine

12 BT 09 L17 Hazardous waste management

13 BT 09 L18 Analytical techniques in biotechnology

14 BT 09 L19 Energy Engineering

15 BT 09 L20 Transport phenomena in bioprocess systems

16 BT 09 L21 Design and analysis of bioreactors

17 BT 09 L22 Modelling and simulation of process plants

18 BT 09 L23 Nanobiotechnology

19 BT 09 L24 Bioethics & Intellectual Property Rights

20 BT 09 L25 Biomaterials


5/129

EN09 301: ENGINEERING MATHEMATICS III(COMMON FOR ALL BRANCHES)

ObjectiveThis course provides a quick overview of the concepts and results in complex analysis

that may be useful in engineering. Also it gives an introduction to linear algebra and Fourier

transform which are wealth of ideas and results with wide area of application.

Module I: Functions of a Complex Variable (13 hours)Functions of a Complex Variable Limit Continuity Derivative of a Complex function

Analytic functions Cauchy-Riemann Equations Laplace equation Harmonic Functions Conformal Mapping Examples: Zn, sinz, cosz, sinhz, coshz, (z+1/Z ) Mobius Transformation.

Module II: Functions of a Complex Variable (14 hours)

Teaching scheme Credits: 4

3 hours lecture and 1 hour tutorial er week

GLOBAL ELECTIVES FROM OTHER BRANCHES

1 ME 09 L 24 Industrial safety Engineering

2 ME 09 L 25 Energy engineering and management

3 PE 09 L23 Total quality management

4 PE 09 L 24 Industrial psychology

5 PE 09 L25 Entrepreneurship

6 CS 09 L23 Simulation and modeling

7 CS 09 L24 Computer based numerical methods

8 CH 09 L23 Nano materials and nanotechnology

9 CH 09 L25 Project engineering

10 IT 09 L24 Management information systems


6/129

Definition of Line integral in the complex plane Cauchys integral theorem (Proof of existenceof indefinite integral to be omitted) Independence of path Cauchys integral formula Derivatives of analytic functions (Proof not required) Taylor series Laurent series Singularities and Zeros Residues Residue Integration method Residues and Residue

theorem Evaluation of real integrals.

Module III: Linear Algebra (13 hours) - Proofs not requiredVector spaces Definition, Examples Subspaces Linear Span Linear Independence

Linear Dependence Basis Dimension Ordered Basis Coordinate Vectors TransitionMatrix Orthogonal and Orthonormal Sets Orthogonal and Orthonormal Basis Gram-Schmidt orthogonolisation process Inner product spaces Examples.

Module IV: Fourier Transforms (14 hours)Fourier Integral theorem (Proof not required) Fourier Sine and Cosine integral representations

Fourier Transforms Fourier Sine and Cosine Transforms Properties of Fourier Transforms.

Internal Continuous Assessment (Maximum Marks-30)

60% - Tests (minimum 2)30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz,

literature survey, seminar, term-project, software exercises, etc.10% - Regularity in the class

University Examination Pattern

PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks

All questions are compulsory. There should be at least onequestion from each module and not more than two questions

from any module.

PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks

Candidates have to answer four questions out of six. Thereshould be at least one question from each module and not morethan two questions from any module.

PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks

Two questions from each module with choice to answer one

question.

Maximum Total Marks: 70

Text Books

Module I:Erwin Kreysig,Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc.

Sections: 12.3, 12.4, 12.5, 12.6, 12.7, 12.9Module II:Erwin Kreysig,Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc.Sections: 13.1, 13.2, 13.3, 13.4, 14.4, 15.1, 15.2, 15.3, 15.4

Module III:Bernaed Kolman, David R Hill, Introductory Linear Algebra, An Applied First Course, Pearson

Education.Sections: 6.1, 6.2, 6.3, 6.4, 6.7, 6.8, Appendix.B.1Module IV:Wylie C.R and L.C. Barrett,Advanced Engineering Mathematics, McGraw Hill.

Sections: 9.1, 9.3, 9.5


7/129


8/129

EN09 302: HUMANITIES AND COMMUNICATION SKILLS(COMMON FOR ALL BRANCHES)

Objectives

To identify the most critical issues that confronted particular periods and locations in

history To identify stages in the development of science and technology

To understand the purpose and process of communication

To produce documents reflecting different types of communication such as technical

descriptions, proposals ,and reports

To develop a positive attitude and self-confidence in the workplace and

To develop appropriate social and business ethics.

Module I (14 hours)Humanities, Science and Technology: Importance of humanities to technology, education andsociety- Impact of science and technology on the development of modern civilization.Contributions of ancient civilization: Chinese, Indian, Egyptian and Greek.

Cultural, Industrial, Transportation and Communication revolutions.Advances in modern India: Achievements in information, communication and space technologies.

Module II (16 hours)

Concept of communication: The speaker/writer and the listener/reader, medium ofcommunication, barriers to communication, accuracy, brevity, clarity and appropriatenessReading comprehension: Reading at various speeds, different kinds of text for different purposes,reading between lines.

Listening comprehension: Comprehending material delivered at fast speed and spoken material,intelligent listening in interviewsSpeaking: Achieving desired clarity and fluency, manipulating paralinguistic features of speaking,task oriented, interpersonal, informal and semi formal speaking, making a short classroom

presentation.Group discussion: Use of persuasive strategies, being polite and firm, handling questions and

taking in criticisms on self, turn-taking strategies and effective intervention, use of bodylanguage.

Module III (16 hours)

Written Communication : Note making and taking, summarizing, notes and memos, developingnotes into text, organization of ideas, cohesion and coherence, paragraph writing, orderinginformation in space and time, description and argument, comparison and contrast, narratingevents chronologically. Writing a rough draft, editing, proof reading, final draft and styling text.

Technical report writing: Synopsis writing, formats for reports. Introductory report, Progressreport, Incident report, Feasibility report, Marketing report, Field report and Laboratory testreportProject report: Reference work, General objective, specific objective, introduction, body,

illustrations using graphs, tables, charts, diagrams and flow charts. Conclusion and references

Preparation of leaflets, brochure and C.V.


2 hours lecture and 1 hour tutorial per week


9/129

Module IV (14 hours)Human relations and Professional ethics: Art of dealing with people, empathy and sympathy,hearing and listening. Tension and stress, Methods to handle stressResponsibilities and rights of engineers- collegiality and loyalty Respect for authority

Confidentiality conflicts of interest Professional rights, Rights of information, Socialresponsibility.

Senses of ethics variety of moral issues Moral dilemma Moral autonomy Attributes of anethical personality right action self interest



from any module.


Candidates have to answer four questions out of six. There

should be at least one question from each module and not morethan two questions from any module.


Two questions from each module with choice to answer onequestion.


Reference Books

1. Meenakshi Raman and Sangeeta Sharma, Technical Communication- Principles andPractice Oxford University press, 2006

2. Jayashree Suresh and B S Raghavan,Professional Ethics, S Chand and Company Ltd, 20053. Subrayappa,History of Science in India, National Academy of Science, India4. R C Bhatia, Business Communication, Ane Books Pvt. Ltd, 2009

5. Sunita Mishra and C Muralikrishna, Communicatin Skils for Engineers, Pearson Education,2007.

6. Jovan van Emden and Lucinda Becker, Effective Communication for Arts and HumanitiesStudents, Palgrave macmillam, 20097. W C Dampier,History of Science, Cambridge University Press8. Vesilind,Engineering, Ethics and the Environment, Cambridge University Press

9. Larson E,History of Inventions, Thompson Press India Ltd.

10. Bernal J.D, Science in History, Penguin Books Ltd11. Encyclopedia Britannica,History of Science, History of Technology12. Brownoski J, Science and Human Values, Harper and Row


60% - Tests (minimum 2)

30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz,literature survey, seminar, term-project, software exercises, etc.

10% - Regularity in the class




from any module.








10/129

BT09 303 FLUID FLOW OPERATIONS

Teaching Scheme:

4 hrs lecture and 1 hr tutorial per week Credits: 5

Objectives: To impart the basic concepts of fluid statics and dynamics

To study the basic equations of flow

To study about the metering and pumping of fluids

To study about the flow of fluids through packed beds and fluidized beds

Module 1

Introduction to fluid mechanics. Definition of fluid. Physical properties of fluids density,specific weight, specific gravity, compressibility, surface tension, vapour pressure and viscosity. .

Absolute and kinematic viscosity. . Units and dimensions of the properties. Variation ofproperties with temperature and pressure. Rheology of fluids. Classification of fluids.

Fluid statics and applications Pascals law. Hydrostatic equilibrium in gravity and centrifugalfields. Barometric equation. Principle of manometers. Different types of manometers.Principles of continuous gravity and centrifugal decanters. Introduction to fluid flowphenomenon. Reynolds experiment. Reynolds number. Classification of flow.

Module IIBasic equation of fluid flow. Equation of continuity. Equation of motion. Euler equation.Bernoulli equation. Momentum equation. Kinetic energy and fluid friction correction factors.

Laminar flow of incompressible fluids in pipes and conduits. Shear stress and velocitydistribution in circular channels.The friction factor, Hagen-Poiseuille equation. Darcy and Weisbach equation. Concept ofequivalent diameter. Friction factor chart, Friction losses from change in velocity and direction

and loss due to pipe fittings. Turbulent flow, universal velocity distribution equations, frictionfactor- Reynolds number relationship, Nikuradse- Carman Equation, average velocity, Blassius

equation ( derivation not required), Prandtl law.

Module- IIITransportation and metering of fluids. Pipes and pipe standards, tubings, pipe joints, flange,

expansion joints, valves, automatic control valves. Different types of pumps. Description andcomparison. Detailed study of centrifugal pumps.

Velocity diagrams, Developed head. Volumetric flow rate relation. Various losses. characteristiccurves, NPSH. Cavitation, pump priming. Description of rotary pumps, reciprocating pumps,

jet pumps, air lift, and diaphragm pumps. Comparison and application of venturimeter,orifice meter, pitot tube, rotameter, weirs and notches.


11/129

Module IV

Flow past immersed bodies: Drag coefficient, flow through packed bed. Ergun equation.Kozney- Carman equation. Blake Plummer equation. Design of packed beds. Motion of

particles through fluids. Motion from gravitational and centrifugal fields. Terminal settling

velocity. Stokes law. Intermediate law-Newton law. Free and hindered settling Fluidization.The phenomenon of Fluidization, Minimum fluidizing velocity, Advantages and disadvantages offluidized beds. Industrial applications.






All questions are compulsory. There should be at least one

question from each module and not more than two questionsfrom any module.


Candidates have to answer four questions out of six. Thereshould be at least one question from each module and not more

than two questions from any module.



question.


Text /References:

1. Mccabe and Smith : Unit operations in chemical Engg McGraw - Hill.

2. Christie J Geankoplis Transport process and Unit Operations Prentice Hall of India.

3. Streeter Fluid Mechanics. McGraw Hill


12/129

BT 09 304 MICROBIOLOGY

Teaching Scheme:


Objectives:

To develop knowledge of the nature and characteristics of microorganisms

To evaluate the role of microbes in public health and various industries

Module 1

History and development of microbiology. Microbial diversity. Principles of microbialtaxonomy. Morphology ultra structure and reproduction of bacteria, actinomycetes, blue green algae, yeasts, fungi, algae and viruses. Microscopy-Bright field microscopy fluorescence

microscopy, phase contrast microscopy. Electron microscopy. Theory of stainingmicroorganisms. Simple staining. Differential staining- Gram staining, Acid fast staining, sporestaining. Special staining techniques- Flagella staining. negative staining. Sterilization-Control of

microorganisms by physical and chemical agents.

Module II

Nutritional requirements of microorganisms, Nutritional types of bacteria - Formulationof growth medium and different types of media- Synthetic media, complex media Selective

media, differential media, enrichment media, enriched media. Pure culture techniques- Spreadplate, Pour plate and streak plate, preservation and maintenance of cultures.

Module IIIMicrobial growth growth curve, generation time. Batch culture, Fed batch culture and

continuous culture, Synchronous culture- techniques adopted to generate synchronous culture,

Measurement of microbial growth, Enumeration techniques- cell numbers and cell mass.

Influence of environmental factors on growth

Module IV

Microbiological analysis of water- Test for coliforms, Microbial flora of soil. Interactionsamong soil microorganisms - Mutualism, commensalisms, predation, parasitism, amensalism,competition, symbiosis, Use of micro organisms as biofertilizer and bioinsecticide

Microbial spoilage of foods and preservation of foods-Physical and chemical methods,Food borne diseases and intoxications

Text/Reference Books1. M.J.Pelczar Et Al:Microbiology, Tata Mcgraw Hill

2. Prescott Et Al:Microbiology, Mcgraw Hill, USA3. Tauro, Kapoor &Yadav;An introduction to microbiology, Wiley Eastern Ltd.



literature survey, seminar, term-project, software exercises, etc.



13/129

BT 09 305 BIOCHEMISTRY I

Teaching Scheme:

3 Hrs lecture and 1 hr tutorial Credits: 4

To understand the fundamental aspects of life

To attain knowledge of the elemental composition of biomolecules

Module 1Introduction to biochemistry. A historical perspective. General features of biomolecules.Carbohydrates: structure and properties of monosaccharides, oligosaccharides and

polysaccharides, Ring structure and mutarotation. Homo and heteropolysaccharides.Mucopolysaccharides. Sialic acids. Bacterial cell wall polysaccharides. Glycoproteins,membrane glycoproteins and their biological functions. Blood group substances.

Module IIAminoacids and proteins: structure, nomenclature and properties of aminoacids. General

reactions of aminoacids. Peptide bond. Classification of proteins, Basic understanding of primary,secondary, tertiary and quaternary structure of proteins. Denaturation and renaturation.

Enzymes: Nomenclature and classification of enzymes. Activation energy and transition stateenzyme activity, active sites, theories of enzyme specificity. Role of effectors and cofactors.


PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marksAll questions are compulsory. There should be at least onequestion from each module and not more than two questions

from any module.




PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marksTwo questions from each module with choice to answer onequestion.



14/129

Module IIILipids - classification and structure,essential fatty acids- glycerides,hydrolysis offats,structure and properties of phospholipids and glycolipids. Prostaglandins- structure,biological properties. Cholesterol- structure and biological importance.Module IV

Nucleic acids: structure and properties of Purine and pyrimidine bases. Nucleosides and

nucleotides. Structure of nucleic acids. Base pairing role. Structure and functions of DNA andRNA Double helical model of DNA structure. A, B and Z DNA.Vitamins: Chemistry andbiological functions of fat soluble Vitamin A, Vitamin D, Vitamin K and Vitamin E. Watersoluble Vitamins: B complex and Vitamin c. Biological functions.

Text books / References:

1. A.L. Lehninger:Principles of Biochemistry CBS publications.2. E.L.Smith, R.L.Hill et al:Principles of Biochemistry, Vol I, MC Graw Hill Book Company.

3. Lubert Stryer: Biochemistry, W.H.Frceman co

4. Donald Voet, Judith.G.Voet: Biochemistry, Wiley and sons.






PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marksAll questions are compulsory. There should be at least onequestion from each module and not more than two questions

from any module.


Candidates have to answer four questions out of six. Thereshould be at least one question from each module and not morethan two questions from any module.





15/129

BT 09 306 BIOPROCESS CALCULATIONS.

Teaching Scheme:

3 Hrs lecture and 1 hr tutorial per week Credits: 4

To study the laws regarding gas ,liquid and vapour

To develop understanding about material balance and energy balances

To study the stoichiometry and thermodynamics of microbial growth and product

formation

Module 1

Overview of process industry and bioprocess industry. Definition of unit operations andunit processes. Units and dimensions. SI Units. Fundamental concepts of stoichiometry likemole concept, mass fraction, mole fraction, volume fraction, average molecular weight.Concentration of liquids and solutions.

Fundamental laws for gas, vapour, and liquid , vapour pressure of pure liquids, effect oftemperature on vapour pressure, Classius Claperon equation, Antoine equation.

Ideal gas law, Dalton's law, Amagats law. Real gas properties, Van der Waals equation, criticalstate, reduced variables, compressibility factor, vapour liquid equilibria, Raoults law, andHenrys law. Humidity and saturation. Wet bulb and dry bulb temp. humidity chart

Module -IIMaterial balances. Law of conservation of mass. Material balance without chemical

reactions. Total and component balances, concept of steady state, batch and continuous process.Tie element basis for calculations. Recycling and bypassing operations. Material balance with

chemical reactions. Concept of excess reactant, limiting reactant, conversion, yield, degree ofcompletion.

Module-III

Energy balance. Law of conservation of energy. Components of energy balance equation.Heat and work. Internal energy. Concept of Enthalpy and heat capacity. Enthalpy changes. Heateffects accompanying chemical reactions. Standard heats of reaction, combustion and formation.Hesss law. Effect of temperature on standard heats of reaction. Steady state energy balance

calculations. Steam tables.

Module -IVStoichiometry of microbial growth and product formation. Stoichiometric calculations

elemental balances, degree of reduction. Yield coefficients. Biomass yield. Productstoichiometry. Theoretical oxygen demand. Maximum possible yield.

Thermodynamics of microbial growth. Heat of reaction with oxygen as electron acceptorand with oxygen not the electron acceptor. Energy balance equation for cell culture.Fermentation energy balance equation.


16/129

References:

1. David M HimmelblauBasic principles and calculations in Chemical Engg Prentice Hall

India.2. V.Venkatarmani & N.N.Ananthraman Process calculation Prentice Hall India.3. B I Bhatt & S.M Vora Stoichiometry Tata McGraw Hill.

4. Michael L Shuler & Fikret Kargi Bioprocess Engg. Basic Concepts Prentice - HallIndia.

5. Pauline M Doran Bioprocess Engineering Principles Academic Press.







question from each module and not more than two questionsfrom any module.


Candidates have to answer four questions out of six. Thereshould be at least one question from each module and not more

than two questions from any module.



question.Maximum Total Marks: 70


17/129

BT 09 307 (P) MICROBIOLOGY LABORATORY

Teaching Scheme:

3 hours practical per week Credit: 2

Objectives

To attain knowledge about the morphology and in vitro cultivation of

microorganisms

To study about the microbial analysis of food water and soil

1. Sterilization techniques; Wet heat, dry heat, filtration, disinfection2. Preparation of culture media, cotton plugging and sterilization

3. Culturing of microorganisms: broth, agar, pure culture, streakPlate, pourplate,Spread plate isolation and preservation of bacterial culture.

4. Identification of microorganisms: Staining techniques, Simple staining,Gramstaining,spore, capsule, fungal staining, and biochemical test- Carbohydrate

Fermentation,IMViC, TSI, Urease Test.5. Quantification of microorganisms: counting microscopy, nephelometry /turbidometry,

total N or dry weight.6. Growth curve of bacteria7. Microbiological analysis of water,8. Food microbiology: milk, fermented food. Salmonella in poultry

9. Factors affecting the bacterial growth: effect of temperature and pH10. Clinical microbiology: Normal mouth flora,Antibiotic Assay11. Microbial population in soil12. Isolation of nitrogen fixing organisms.

Sessional work assessments

Lab Practical and Record = 60%

Two tests (2 x 10) = 30%Regularity = 10%

Total marks = 50

Semester end examination

Fair record = 10%Viva voce = 20%Procedure and tabulation form,Conducting experiments and results = 70 %

Total marks = 50


18/129

BT 09 308 (P) BIOCHEMISTRY LABORATORY

Teaching Scheme:

3 hours per week Credits: 2

Objective:

To study about the qualitative and quantitative analysis of biomolecules

1. Units, Volume and weight measurements, concentration units, pH measurement,preparation of buffers.

2. Qualitative tests for carbohydrates and amino acids

3. Estimation of reducing sugars by the Benedicts method.4. Quantitative estimation for amino acids-Ninhydrin method.

5. Protein estimation by Biuret/Folins/Bradford method.6. Acid hydrolysis of proteins and estimation of amino acids by ninhydrin, OPA and PTH.7. Extraction of lipids

8. Saponification of fats9. Estimation of cholesterol10. Estimation of nucleic acids: Precipitation by sodium sulphate, test for ribose and

deoxyribose11. Hydrolysis of ester using papain

12. Trypsin digestion of proteins



Test/s = 30%Regularity = 10%

Total marks = 50

Semester End examinationFair record = 10%

Viva voce = 20%Procedure and tabulation form,Conducting experiments and results = 70 %

Total marks = 50


19/129

FOURTH SEMESTER

EN09 401A: Engineering Mathematics IV(Common for ME, CE, PE, CH, BT, PT, AM, and AN)

ObjectiveThe use of probability models and statistical methods for analyzing data has become

common practice in virtually all scientific disciplines. Two modules of this course attempt to

provide a comprehensive introduction to those models and methods most likely to be encounteredand used by students in their careers in engineering. A broad introduction to some important

partial differential equations is also included to make the student get acquainted with the basicsof PDE.

Module I: Probability Distributions (13 hours)Random variables Mean and Variance of probability distributions Binomial Distribution Poisson Distribution Poisson approximation to Binomial distribution Hyper GeometricDistribution Geometric Distribution Probability densities Normal Distribution Uniform

Distribution Gamma Distribution.

Module II: Theory of Inference (14 hours)Population and Samples Sampling Distribution Sampling distribution of Mean ( known)

Sampling distribution of Mean ( unknown) Sampling distribution of Variance IntervalEstimation Confidence interval for Mean Null Hypothesis and Tests of Hypotheses

Hypotheses concerning one mean Hypotheses concerning two means Estimation of Variances Hypotheses concerning one variance Hypotheses concerning two variances Test of

Goodness of fit.

Module III: Series Solutions of Differential Equations (14 hours)Power series method for solving ordinary differential equations Legendres equation Legendrepolynomials Rodrigues formula Generating functions Relation between Legendrepolynomials Orthogonality property of Legendre polynomials (Proof not required) Frobenius

method for solving ordinary differential equations Bessels equation Bessel functions Generating functions Relation between Bessel functions Orthogonality property of Besselfunctions (Proof not required).

Module IV: Partial Differential Equations (13 hours)Introduction Formation of PDE Complete Solution Equations solvable by direct integration Linear PDE of First order, Legranges Equation: Pp + Qq = R Non-Linear PDE of First Order,F(p,q) =0 , Clairauts Form: z = px + qv + F(p,q) , F(z,p,q) =0 , F 1(x,q) = F2(y,q) Classification

of Linear PDEs Derivation of one dimensional wave equation and one dimensional heatequation Solution of these equation by the method of separation of variables DAlembertssolution of one dimensional wave equation.

Teaching scheme Credits: 43 hours lecture and 1 hour tutorial er week


20/129

Module I:Richard A Johnson, CB Gupta, Miller and Freunds Probability and statistics for Engineers, 7e,Pearson Education- Sections: 4.1, 4.2, 4.3, 4.4, 4.6, 4.8, 5.1, 5.2, 5.5, 5.7

Module II:Richard A Johnson, CB Gupta, Miller and Freunds Probability and statistics for Engineers, 7e,Pearson Education- Sections: 6.1, 6.2, 6.3, 6.4, 7.2, 7.4, 7.5, 7.8, 8.1, 8.2, 8.3, 9.5

Module III:

Erwin Kreysig,Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc.-Sections: 4.1, 4.3, 4.4, 4.5

Module IV:

N Bali, M Goyal, C Watkins, Advanced Engineering Mathematics, A Computer Approach, 7e,Infinity Science Press, Fire Wall Media- Sections: 16.1, 16.2, 16.3, 16.4, 16.5, 16.6, 16.7, 16.8,16.9

Erwin Kreysig,Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc.Sections: 11.2, 11.3, 11.4, 9.8 Ex.3, 11.5

Reference books

18. William Hines, Douglas Montgomery, avid Goldman, Connie Borror,Probability and Statisticsin Engineering, 4e, John Wiley and Sons, Inc.

19. Sheldon M Ross, Introduction to Probability and Statistics for Engineers and Scientists, 3e,Elsevier, Academic Press.

20. Anthony Croft, Robert Davison, Martin Hargreaves, Engineering Mathematics, 3e, PearsonEducation.

21. H Parthasarathy,Engineering Mathematics, A Project & Problem based approach, Ane Books

India.22. B V Ramana,Higher Engineering Mathematics, McGrawHill.23. Sarveswara Rao Koneru,Engineering Mathematics , Universities Press.

24. J K Sharma, Business Mathematics, Theory and Applications, Ane Books India.

25. John bird,Higher Engineering Mathematics , Elsevier, Newnes.26. M Chandra Mohan, Vargheese Philip, Engineering Mathematics-Vol. I, II, III & IV., Sanguine

Technical Publishers.

27. Wylie C.R and L.C. Barret,Advanced Engineering Mathematics, McGraw Hill.28. V R Lakshmy Gorty,Advanced Engineering Mathematics-Vol. I, II, Ane Books India.29. Sastry S.S.,Advanced Engineering Mathematics-Vol. I and II, Prentice Hall of India.

30. Michael D Greenber Advanced En ineerin Mathematics Pearson Education.






21/129

EN09 402: Environmental Science(Common for all branches)

Objectives To understand the problems of pollution, loss of forest, solid waste disposal,

degradation of environment, loss of biodiversity and other environmental issues andcreate awareness among the students to address these issues and conserve the

environment in a better way.

Module I (8 hours)The Multidisciplinary nature of environmental science, Definition-scope and importance-need forpublic awareness. Natural resources, Renewable and non-renewable resources:

Natural resources and associated problems-forest resources: Use and over exploitation,deforestation, case studies. Timber extraction, mining, dams and their defects on forests and tribalpeople.- water resources: Use and over utilization of surface and ground water, floods,drought ,conflicts over water, dams-benefits and problems.- Mineral resources: Use and

exploitation,environmental effects of extracting and using mineral resources, case studies.- Foodresources: World food problems, changes caused by agriculture over grazing, effects of modernagriculture, fertilizer-pesticide problems, water logging,salinity,case studies.-Energy resources:

Growing energy needs, renewable and non-renewable energy resources, use of alternate energyresources, Land resources: Land as a resource, land degradation, man induced land slides, soilerosion and desertification.

Module II (8 hours)Ecosystems-Concept of an ecosystem-structure and function of an ecosystem producers,consumers, decomposers-energy flow in the ecosystem-Ecological succession- Food chains, food

webs and Ecological pyramids-Introduction, types, characteristics features, structure and function


2 hours lecture and 1 hour tutorial per week




question from each module and not more than two

questions from any module.


Candidates have to answer four questions out of six.

There should be at least one question from each module

and not more than two questions from any module.


Two questions from each module with choice to answer


22/129

of the following ecosystem-Forest ecosystem- Grassland ecosystem Desert ecosystem-Aquaticecosystem(ponds, streams, lakes, rivers, oceans , estuaries)Biodiversity and its considerationIntroduction- Definition: genetic , species and ecosystem diversity-Biogeographical;

classification of India value of biodiversity: consumptive use, productive use, social ethical ,aesthetic and option values Biodiversity at Global, national , and local level-India at mega

diversity nation- Hot spot of biodiversity-Threats to biodiversity: habitat loss, poaching of wildlife, man , wild life conflicts Endangered and endemic species of India-Conservation of

biodiversity : In-situ and Ex-situ conservation of biodiversity.

Module III (10 hours)Environmental pollution

Definition-Causes, effects and control measures of Air pollution-m Water pollution soilpollution-Marine pollution-Noise pollution-Thermal pollution-Nuclear hazards-Solid waste

management: Causes, effects and control measures of urban and industrial wastes-Role of anindividual in prevention of pollution-pollution case studies-Disaster management: floods, earthquake, cyclone and landslides-Environmental impact assessment

Module IV (10 hours)Environment and sustainable development-Sustainable use of natural resources-Conversion ofrenewable energy resources into other forms-case studies-Problems related to energy and Energyauditing-Water conservation, rain water harvesting, water shed management-case studies-Climate

change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust-Wasteland reclamation-Consumerism and waste products-Reduce, reuse and recycling of products-Value education.

Text Books1. Clark, R.S.Marine pollution, Clanderson Press Oxford.

2. Mhaskar A. K. Matter Hazrdous, Techno-science Publications.3. Miller T. G. Jr., Environmental Science, Wadsworth Publishing Co.

4. Townsend C., Harper J, Michael Begon, Essential of Ecology, Blackwell Science5. Trivedi R. K., Goel P. K., Introduction to Air Pollution, Techno-Science Publications.

Reference Books.1. Raghavan Nambiar,K Text book of Environmental Studies,Nalpat Publishers Kochi2. Bharucha Erach, Biodiversity of India, Mapin Publishing Pvt. Ltd., Ahmedabad 380 013,

Email: [email protected]. Cunningham, W.P., Cooper, T.H., Gorhani, E & Hepworth, M.T. 2001Environmental

encyclopedia Jaico publ. House Mumbai 1196p4. Down to Earth, Centre for Science and Environment

5. Hawkins, R.E. Encyclopedia of Indian Natural History, Bombay Natural History Society,Bombay

6. Mckinney, M.L. & School, R.M. 1996. Environmental Science system & Solutions, Webenhanced edition, 639p.

7. Odum, E.P. 1971. Fundamentals of Ecology. W.B.Saunders Co. USA, 574p8. Rao, M.N. & Datta, A.K 1987. Waste Water treatment. Oxford & IBH Publ. Co. Pvt. Ltd.,

345p9. Survey of the Environment, The Hindu Magazine10. Wagner.K.D. 1998. Environmental Management. W.B. Saunders Co. Philadelphia, USA

499p
mailto:[email protected]:[email protected]


23/129

BT 09 403 CHEMICAL REACTION ENGINEERING

Teaching Scheme:

4 Hrs lecture and 1 hr tutorial credits: 5

Objectives:

To impart the basic concepts of reaction kinetics

To develop knowledge for design of ideal reactors

To study about non-isothermal reactor design

To study about the fundamentals of non-ideal reactors and heterogeneous

catalytic reactors

Module I
















24/129

Introduction to chemical reaction engineering. Classification of chemical reactions andreactors. Variables affecting the rate of reaction. Definition of reaction rate. Kinetics ofhomogeneous reaction. Concentration dependent term and temperature dependent term.Interpretation of batch reactor data. Constant volume batch reactor. Varying volume batch

reactor.

Module - IIIntroduction to reactor design. Ideal reactors for a single reaction. Design for single

reaction and multiple reactions. Multiple reactor systems. Size comparison of reactors. Recyclereactor. Autocatalytic reactor.

Module III

Heat effects in reactors. Non isothermal reactor design. General graphical designprocedure. Energy balance for batch, mixed flow and plug flow reactor isothermal, adiabatic

and nonadiabtic operation. Optimum temperature progression. Multiple steady states, criteria forstability of reactors.Basics of non-ideal flow. Residence time distribution. Measurement of the RTD. Pulse and step

input . C,E,F curves. Calculation of mean residence time. RTD in ideal reactors.RTD in laminar

flow reactor. Segregation model and conversion in non-ideal reactors ( Dispersion and tanks-in-series models are not included).

Module IV

Heterogeneous catalytic processes. Classification of catalysts, promoters, inhibitors,catalyst poisons, Adsorption. Rates of adsorption, desorption, and surface reaction. Rateequations for fluid-solid catalytic reactions.. Mass Transfer between fluid and catalyst surface.Internal transport effects. Effectiveness factor for a straight cylindrical catalyst pore for a first

order reaction. Commercially significant types of heterogeneous catalytic reactors (descriptiononly) like fixed bed reactor, trickle bed reactor, moving bed reactor, fluidized bed reactor, slurryreactor.

Text book/References

1. Octave Levenspiel Chemical Reaction Engg Wiley student Education.2. H.Scott Fogler Elements of Chemical Reaction Engg. Pearson Prentice Hall.3. J.M.Smith Chemical Engineering Kinetics McGraw Hill International.

Internal Continuous Assessment (Maximum Marks-30)60% - Tests (minimum 2)




25/129

BT 09 404 BIOCHEMISTRY II

Teaching Scheme:


Objectives:

To impart complete understanding of biochemical processes associated with theliving cell

To enable the students to see how metabolic pathways communicate with each

other

Module IBioenergetics: Redox potential, biological oxidation, respiratory control. Hub of metabolism:glycolysis: reactions, energetics and regulation.TCA Cycle: reactions, energetics and regulation.Gluconeogenesis pathway: Significance, reactions and regulation. Glycogen metabolism.

Maintenance of blood glucose levels.Oxidative phosphorylation: Electron transport chain mitochondria and components of electrontransport system (Role of complexes I-IV, FIFO ATPase) Chemiosmotic coupling, uncouplers andinhibition of oxidative phosphorylation. Pentose phosphate pathway: significance, reactions and

regulation.

Module IIMetabolism of amino acids: Essential and non-essential amino acids, overview of degradation

and biosynthesis of amino acids. Transamination reactions,biosynthesis of aromatic amino acids













26/129

and biosynthesis of cysteine, histidine and serine. Urea cycle: reactions, regulation and itslinkage with the citric acid cycle.

Module III

Metabolism of lipids: fatty acid oxidation, ketone bodies, ketosis, biosynthesis of fatty acids andtriglycerides. Biosynthesis of phospholipids. Biosynthesis of Cholesterol and significance.

Module IVMetabolism of nucleic acid: Denovo biosynthesis of Purine and pyrimidine nucleotides,regulation of Purine and pyrimidine nucleotide biosynthesis. Salvage pathways of Purine andpyrimidines. Formation of deoxyribonucleotides. Catabolism of purines and pyrimidines.

Tex books/References

1. LehningerPrinciples of biochemistry, David L.Nelson and Michal M Gox, W.H.Freemanand co.

2. Donald Voet, Judit G Voet, Charlotte W Pratt Fundamentals of biochemistry.3. Lubert Stryer, Biochemistry. W.H.Freeman & Co.4. Robert K.Murray, Daryl K.Grammer.Harpers Illustrated Biochemistry. McGraw Hill
















27/129

BT 09 405 CELL BIOLOGY

Teaching Scheme:

3 hrs lecture and one hr tutorial per week Credits: 4

Objectives:

To impart knowledge of the components of cells and how cells work

To study the cells : their physiological properties, their structure, the organelles

they contain, life cycle interaction, division and death at microscopic and

molecular level

Module IDiscovery of cells. Basic properties of cells. Cell theory. Cell complexity. Different classes ofcells. Prokaryotic & Eukaryotic system Prokaryotic & Eukaryotic cells. Importance of carbon

and water. Plasma membrane structure and function. Cytoplasm and cytoskeleton,Microtubules, microfilaments and intermediate filaments .

Module IIStructure and functions of Nucleus, Endoplasmic reticulum, Golgi complex, Lysosomes,Peroxisomes, Chloroplast & Mitochondria. Photosynthesis- introduction to photosystems , light

reaction and dark reaction. Protein glycosylation, sorting and transport.

Module IIIOverview of the cell cycle, Interphase. Mitosis, Meiosis and cytokinesis. Animal cell

and yeast cell division. Cell cycle control and check points. General characteristics of celldifferentiation. Historical events in cell differentiation Cytoplasmic determinants.

Nucleoplasmic interactions. Stem cell differentiation and its biological importance.

Module IVPassive and active transport, Permeases, Na+/K+ pump, ATP ase pumps, Lysosomal & vacuolar

membrane, ATP dependent proton pumps. Co-Transport Symport. Antiport. Transport intoprokaryotic cells. Endocytosis and Exocytosis.

Receptors: Cytosolic, Nuclear & Membrane bound receptors. Examples and types of receptors.Concept of secondary messengers, cAMP, cGMP

Text/References books1. Geoffrey M cooper and Robert E Hansman the cell A molecular approach. ASM press.2. De Robertis and De Robertis. Cell and Molecular Biology Waverly Pvt Ltd.

3. Gerald Karp Cell and Molecular Biology Wiley publishers.4. Kimball.T.W Cell Biology Wesley publisher.5. Becker, Kleinsmith, Harden The world of Cell. Pearson Publishers.


28/129



30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz,














29/129

B T 09 406 MECHANICAL OPERATIONS

Teaching Scheme:


Objectives:

To impart the basic concepts of size reduction and the knowledge about the size

reduction equipments

To study about the various mechanical separation operations

Module ICharacterization of solid particles. Sieve analysis, particle size distribution , cumulative

and differential methods of analysis,mean diameter, specific surface area Microscopic counting,

pipette analysis. hydrometer analysis. Size reduction, equipment used for primary and secondarystage size reduction. jaw crusher, hammer mill, ball mill, rod mill, disk attrition mill, fluid energymill. Laws of size reduction. Selection of equipments. Industrial screen, effectiveness ofscreens, capacity of screens. Closed and open circuit grinding. Wet and dry grinding.

Module IIFiltration. Constant rate filtration and constant pressure filtration. Batch and continuous

filtration. Sand filter, plate and frame filter press, leaf filter, rotary vaccum filter, incompressiblecake, cake porosity, filter aids, methods of application, optimum time cycle.

Principle of centrifugal filtration. Suspended batch centrifuge. Automatic batch centrifuges.

Module III

Sedimentation. Settling theory. Equipment for sedimentation thickeners. Clarifier andthickener design. Kynch theory. Determination of thickener area. Sedimentation principles. .Equilibrium sedimentation. Sedimentation coefficient. Equivalent time. Production centrifuges

tubular bowl centrifuges. Ultra centrifugation. Flocculation and sedimentation.Disk stack bowl centrifuge centrifugation theory. Cell recovery in a disk-stack

centrifuge.

Module IVCross flow filtration (CFF) Micro filtration and ultra filtration. Applications of CFF.

Types of membranes permeate flux for ultra filtration, concentration polarization in ultrafiltration, Micro filtration. Filter media in cross flow filtration. Equipments in cross flow

filtration. Membrane fouling. Scale up and design of cross flow filtration. Batch and

continuous ultra filtration system.

Text/References

McCab&Smith: Unit operations in Chemical Engg McGraw Hill

Christe J Geankoplis Transport Process and Unit Operations. Prentice Hall of India.Badger and Benjaro, Introduction to Chemical Engineering , Mc Graw Hill


30/129


31/129

8. Sieve analysis-determination of particle size.9. Effectiveness of a screen10. Pipette analysis

11. Determination of the mean specific cake resistance and compressibility factor using abatch leaf filter.

12. Batch sedimentation in a slurry

13. Flocculation screening14. Cross flow filtration.15. Study of Jaw crusher16. Study of rotary vaccum filter.

BT 08 408(P) BIOANALYTICAL TECHNIQUES LAB

Teaching Scheme:

3 hours practical per week credits 2

Objective:

To provide experience in various bioanalytical techniques in biotechnolog

y

1. Precision, accuracy and validity of an experiment. Analysis and presentation of data.

2. Colorimetry and spectrophotometry3. Verification of Beer-Lamberts law-using UV-Vis spectrophotometer.

a. Change in absorbance with concentration of potassium permanganate.b. Absorption maxima change in absorbance in potassium permanganate with

wavelength.



Test/s = 30%Regularity = 10%

Total marks = 50

Semester End examination


Total marks = 50


32/129

c. Concentration of two components in a binary mixture. Absorption of light bypotassium dichromate and potassium permanganate.

d. Change in absorbance of albumin and DNA solution with wave length.4. Absorption spectra of nucleotides

5. Separation of amino acids by paper chromatography Determination of Rf value.6. Extraction of lipids and separation using thin layer chromatography

7. Column chromatography8. Determination of molecular weight of an enzyme by gel filtration.9. Separation of protein by SDS page.10. Separation of nucleic acids by agarose gel electrophoresis11. Biotin labeling of cellular constituents.



Test/s = 30%

Regularity = 10%Total marks = 50



Total marks = 50


33/129

FIFTH SEMESTER

BT09 501 MASS TRANSFER OPERATIONS

Teaching Scheme:


Objectives:

To impart the basic concepts of mass transfer

To develop an understanding of different separation processes like

distillation,extraction,drying and crystallisationModule-IClassification of mass transfer operations Diffusional mass transfer Ficks law. One component

transferring to non-diffusing component and equimolar counter diffusion diffusivity estimation.The mass transfer coefficient. Dimensionless groups in mass transfer. Theories of mass transfer.Film theory, Penetration theory, surface renewal theory, the boundary layer theory. Momentum,heat and mass transfer analogies. Interphase mass transfer. The two-film theory, the overall mass

transfer coefficient. General features of equipment for mass transfer Tray tower, packed tower,bubble column, wetted wall tower, and spray tower.

Module IIBasic concepts of distillation vapor liquid equilibrium, the Raoults law, Relative volatility,

Deviations from ideality. Flash vapourisation of a binary mixture, simple distillation, steamdistillation, continuous fractionation, McCabe-Thiele method, Ponchon, - Savarit method,minimum reflux ratio. Total reflux, optimum reflux ratio.

Module III

Liquid liquid extraction applications, Liquid liquid equilibrium. Solvent selection, Designcalculations for stage wise extraction, single stage and multi stage operation, crosscurrent andcountercurrent operations, liquid liquid extraction equipment. Solid liquid extraction,(leaching). Contacting equipments. Solid liquid extraction equilibrium. Batch contact. Cross-

current and countercurrent extraction calculations. Super critical fluid extraction.

Module-IV

Drying of wet solids. Classification of drying equipment. Drying equipment. Dryingcalculations cross circulation drying and through circulation drying. Material and energybalance in a continuous dryer. Freeze drying. Drying time in a continuous counter current dryer.

Crystallization. Principles of crystallization. Solid-liquid phase equilibrium, Nucleation andcrystal growth. Effect of impurities on crystal formation Fractional crystallization, caking ofcrystals. Crystallization equipment. Adsorption: Adsorption Isotherm, Freudlich, Langmuir andBET equation. Application to single stage and multistage operation.


34/129

Text book/References

1. Treybal Mass Transfer operations McGraw Hill International

2. McCabe-Smith Unit operations of Chemical Engg McGraw Hill International

3. Binay K Dutta Mass Transfer and Separation processes.

4. Badger and Benjaro,Introduction to Chemical Engineering, Mc- Graw Hill










PART B: Analytical/Problem solving questions 4 x 5 marks=20 marksCandidates have to answer four questions out of six.






35/129

BT 09 502 MOLECULAR BIOLOGY

Teaching Scheme:

3 Hrs Lecture and 1 hr tutorial per week Credit: 5

Objectives:

To get a proper understanding about the central dogma of the molecular

mechanism

To study the analytical techniques used in molecular biology

Module IIntroduction to genetics: Mendelian inheritance. Segregation at one locus. Segregation at twoloci genetic mapping.

Structure of DNA. Nucleosome. Chromatin organization. Chromosome structure. Plasmidstypes of plasmid. Structural differences between DNA and RNA.DNA replication: Enzymes involved. Mechanism of replication. Regulation damage and repair,

rolling circle replication. Plasmid replication Telomerase.

Module IIConcept of the gene: Gene structure and architecture. Central dogma.

Genetic code: An overview of genetic code Fidelity of translation, Wobble hypothesis.Ambiguity of genetic code. Deviation from standard.Genomes and mapping :Genomes, ploidy and chromosome number. Physico-chemical propertyof the genome. Genome organization, gene mapping. Physical mapping. Comparative genomics.

Module IIIGene transfer in bacteria: conjugation, transformation, transduction. Gene expression.Role of RNAs, enzymes and other factors. Mechanism of transcription and translation in

prokaryotes and eukaryotes. Post- translational modifications.Regulation of gene expression regulation of transcription. Different mechanisms Regulatory

proteins. Induction and repression. Concept of operon. . Examples (Lac and Trp operon) Positiveand negative regulations. Post-transcriptional regulation.

Module IV

Mutation: Mutagenesis. Types of mutation. Classes of mutagens Replica plating. Mutation ingenetic analysis. Site directed mutagenesis Molecular biology Techniques Electrophoresis(Agarose gel and PAGE). Blotting techniques (Southern, Northern, Western) PCR and variants.

Text Books/References

1. Freifelder Molecular Biology James and Bartletyt Publishers. Ire2. Banjamin Levin: Genes VI, Oxford University Press, Oxford, New York.3. Bruce Albert and James, D Watson: Molecular Biology of the cell. Garland publishing.


36/129

BT09 503 ENZYME SCIENCE & ENGG.

Teaching Scheme:

3 Hrs lecture and 1 hr tutorial per week Credit: 4

Objective:

To impart the basic concepts of enzymes and the reactors involved in free and

immobilized enzyme system

To understand the kinetics and physicochemical characteristics of enzymes

Module I

Classification of enzymes, commercial application of enzymes in food, pharmaceuticaland other industries. Enzymes for analytical and diagnostic applications. Production and

purification of crude enzymes. Extracts from plant, animal and microbial sources. Methods of

















37/129

characterization of enzymes. Development of enzymatic assays. Hydrolysis of starch andcellulose using enzymes.

Module-IIMechanism of Enzyme action, Concept of active site, enzyme-substrate complex and

enzyme action, Simple enzyme kinetics with one and two substrates. Michaelis-Mentenkinetics. Evaluation of parameters in the Michaelis-Menten kinetics Equation. Types ofinhibition. Influences of pH, temperature, fluid forces, chemical agents and irradiation on enzymeactivity, deactivation kinetics.

Module III

Enzyme immobilization. Physical and chemical techniques for enzyme immobilizationadsorption, matrix entrapment, encapsulation, cross linking, covalent binding. Advantages anddisadvantages of different immobilization techniques. Over view of application of immobilizedenzyme systems.

Mass transfer effects in immobilized enzyme systems. Analysis of film and porediffusion effects on kinetics of immobilized enzyme reactions. Effectiveness factors.

Module IV

Batch Operation of a stirred reactor Time course for batch enzyme reaction. Continuousoperation in a stirred tank reactor.

Immobilized enzyme reaction in a CSTR and plug flow reactor. Design of immobilizedenzyme reactors packed bed, fluidized bed and membrane reactors. Enzyme biosensors,

application of enzymes in analysis, design of enzyme electrodes and their application in industry,health care and environment.

Text books/References

1. Zubay G, Biochemistry, Maxwell Macmillon International Education

2. Gerharts, W,Enzymes in industry Production and application.3. Tailer, R.F. Protein Immobilization Fundamentals and applications.

4. Pauline M Doran Biopriocess Engg. Principles Academic press

5. James E Bailey & David F Ollis Biochemical Engineering Fundamentals Mc Graw HillBook Company.






38/129

BT 09 504 THERMODYNAMICS AND HEAT TRANSFER

Teaching Scheme:3 Hrs lecture and 1 hr tutorial per week Credit: 4Objective:

To impart the basic concepts of thermodynamics and heat transfer

To study the design of various types of heat exchangers

Module I

Introduction and fundamental concepts of thermodynamic terms. First law ofthermodynamics. Work equivalent of heat. Classification of energy, thermodynamic state

function and path function. Enthalpy and specific heat. Application of first law to steady stateflow processes and reversible process.

Limitations of first law. Statement of second law. Entropy. Heat reservoirs, heat enginesand heat pumps. Applications of the laws of thermodynamics to refrigeration, liquefaction

process.

Module IIModes of heat transfer: conduction, convection and radiation. Heat transfer by conduction.

Fouriers law. Thermal conductivity Steady state conduction through a composite solid. Steadystate conduction through cylinder and spheres

Heat transfer coefficient: Convective heat transfer and the concept of heat transfercoefficient. Overall heat transfer without heat transfer between fluids separated by a plane wall.

Heat transfer between fluids separated by a cylindrical wall.

Module III




question from each module and not more than twoquestions from any module.








39/129


40/129

BT 09 505 BIOINFORMATICS

Teaching Scheme:

3 hours lecture and 1 hour tutorial per week Credits: 4

Objective:

To understand the fundamental principles of bioinformatics and using the

knowledge to tackle various research problems in molecular biology

Module IScope of Bioinformatics Internet basics, www, protocols FTP Telnet HTTP, Elementarycommands in UNIX, Introduction to PERL (Basic commands, pattern and string matching),

BIOPERL (Programme, to transcribe and translate nucleotide sequences). Installation of bioperland its applications.

Module II

Introduction of databases, Biological databases and their ruse, Databanks nucleotide databanks Genbank, NCBI, EMBI, DDBJ Protein databanks sequence databanks PIR, SWISSPROT, TrEMBL structural databases PDB, SCOP, CATH, SSEP, CADB, Pfam and GDB. Data base search, sequence submission,

Sequin, Bankit, Sakura, Database search FASTA-BLAST.

Module III













41/129

Sequence alignment, Pairwise alignment Dynamic programming, Multiple sequence alignment.Methods of MSA, Clustal W, Phylogenetic analysis. Tree building methods Distance,Parsimony, Maximum likelihood, Phylogenetic Software, Phylip. Scoring Matrix PAM,BLOSUM.

Module IVSpecial topics in bioinformatics

Methods for prediction of secondary and tertiary structures of proteins knowledge based structureprediction: fold recognition, ab initio methods for structure prediction, Comparative protein

modeling, Gene predictions, Genscan, Precustes, Grail, DNA Mapping and sequencing. Mapalignment Shotgun DNA sequencing Sequence assembly. Protein structure visualization.

Reference:

1. Andreas D.Baxevanis, B.F.Francis Oouellette, Bioinformatics, Wiley.2. Dam Gusfield.Algorithms on Strings Trees and Sequences, Cambridge University Press.3. D.Mount, Bioinformatics Sequence Analysis. Cold Spring Harbor Laboratory.












There should be at least one question from each moduleand not more than two questions from any module.




42/129

BT 09 506 PROCESS INSTRUMENTATION

Teaching Scheme:


Objectives:

To impart the basic ideas about instruments

To evaluate the operating principles of different instruments

To study the instrumentation and control of bioprocesses

Module IIntroduction Definition of instrumentation Concept of an instrument. Functional elements and

functions of an instrument Classification of instruments. Static and dynamic characteristics ofmeasuring instrument. Accuracy, reproducibility, sensitivity, static error, dead zone, dynamic

error, fidelity, lag, speed of response.Sensing elements - various types, sensors for temperature, pressure and fluid flow, transducers,

their principles and working, transmission methods, indicating and recording means.Temperature measurements, temperature scales, basic principles and working of

thermometers-mercury-in-glass thermometers, resistance thermometers, thermocouples, opticalpyrometers, radiant pyrometers, ranges of different types of temperature measuring instruments.Sources of errors and precautions to be taken in temperature measurements.

Module IIPressure measurement. Principles of working of manometers. Various types of

manometers. McLeod gauge, Knudsen gauge, bourdon gauge, bellow, diaphragm. Transducersof electrical and mechanical types. Piezo-electric manometers, thermal conductivity gauges,

ironisation gauge, high pressure measuring instrument.Density measurement using constant volume hydrometer and air pressure balance method

Gas density detector and gas specific gravity measuring system. viscosity measurement.Measurement of pH, Liquid level measurements.

Module III

Flow measurement using head type flow meters based on differential pressuremeasurement. Orifice meter, venturimeter, flow nozzle and pitot tube. Electromagnetic flow

meters. Variable area meters like rotameter. Mechanical flowmeters of positive displacementtype like rotating disk and turbine type and anemometers.

Moisture content determination by thermal drying. Instruments for measuring humiditylike hygrometer, psychrometer, and dew-point apparatus. Composition analysis usingspectroscopic methods like absorption, emission and mass spectrometers. Gas analysis bythermal conductivity, polarography and chromatography.

Module IVInstrumentation for bioprocesses. Physical and chemical sensors for the medium and

gases. Medium chemical sensors. Gas analysis. On line-sensors for cell properties. Offline


43/129

analytical methods. Measurement of medium properties. Analysis of cell populationcomposition. Flow cytometry. Data analysis, data smoothing and interpolation. State andparameter estimation. Development of P&I diagrams for flow systems, level, PH control.Temperature control, heat exchangers, distillation columns and reaction systems.


1. D.P.Eckman.Industrial instrumentation , Wiley Eastern

2. Fribance,Industrial Instrumentation fundamentals, TMH Edition

3. R.K.Jain.Mechanical and Industrial Measurements, Khanna Publishers.4. Bailey & Ollis. Biochemical Engineering Fundamentals McGraw Hill Book Company

5. Beckworth and Buck:Measurement Systems

















44/129

BT 09 507(P) HEAT AND MASS TRANSFER LAB

Teaching Scheme:

3 hours practical per week Credits: 2

Objective:

To study about the experiments in heat and mass transfer

1. Conduction -Determination of thermal conductivity

2. Heat transfer through composite wall3. Heat transfer in natural conviction.4. Heat transfer in forced convection5. Double pipe heat exchanger

6. Shell and tube heat exchanger

7. Simple distillation8. Steam distillation9. Sieve place distillation column

10.Solid -liquid extraction Bonnotto type11.Solid-liquid extraction- packed bed type

12.Ternary liquid equilibrium13. Leaching simple leaching, cross current and counter current leaching.14. Rotary dryer15. Adsorption isotherms

16. Batch crystallizer



Test/s = 30%Regularity = 10%Total marks = 50

Semester End ExaminationFair record = 10%Viva voce = 20%Procedure and tabulation form,Conducting experiments and results = 70 %

Total marks = 50


45/129

BT 09 508 (P) BIOPROCESS ENGINEERING LAB

Teaching Scheme:

3 hours practical per week Credits: 2

Objectives:

To do experiments based on enzymes and microbial cells

To study about bioreactors and mass transfer effects

1. Enzyme isolation and assay of enzymatic activity.2. Estimation of Michaelis Menten parameter effect of pH and temperature on enzyme

activity.3. Techniques of enzyme immobilization

4. Bio-conversion studies with immobilized enzyme reactors.5. Culturing of different types of micro organisms (Bacteria, Yeast, fungi) used in the

production of commercially imported products.6. Formulation of simple and complex culture media7. Estimation of biomass, substrate and product analysis8. Study of growth, substrate utilization and product formation kinetics in shake flask cultures

9. Growth of micro organisms-estimation of Monod parameters10. Production of secondary metabolite in synthetic and complex industrial media.

11. Mass transfer rate determination in bio reactors dynamic gassing out method.12. Oxygen transfer rate in diffused air system (aeration unit)13. Aerobic bioreactor14. Anaerobic bioreactor

15. Screening of process variable-Single dimensions research, Plackett Burman design16. Study of rheology of fermentation broth and power determination



Test/s = 30%Regularity = 10%Total marks = 50



Total marks = 50


46/129

SIXTH SEMESTER

BT 09 601 GENETIC ENGINEERING

Teaching Scheme:


Objectives:

To impart the basic concepts of genetic engineering

To impart the knowledge of various techniques involved in genetic engineering

Module- IIntroduction to genetic engineering. Tools of genetic engineering. Restriction enzymes.

DNA modifying enzymes. Principles of molecular cloning. Sources of DNA for cloning.Isolation of DNA. Mechanism of DNA cleavage, and end modifications. Mechanism of DNAjoining. CDNA cloning. Screening strategies.

Module - IISalient features of vectors. Plasmids (plasmid biology, purification of plasmid DNA,

Desirable properties of plasmid closing vehicles)

Bacteriophages (Bateriophage) others (cosmids BACS, PACS, YACS)

Eucaryotic vectors. Expression vectors. Shuttle vectors. Ti plasmid of agrobacteria. DNAtransfer to host: Chemical transfection, lipofection, electroporation, microinjection, gene gun,

uses of viruses.

Module - IIIExpression of closed genes: Rationale for expression. Stability of expression vector.

Functionability of expression system. Expression signals. Expression strategies. Native andfusion proteins.

Construction of libraries: c DNA library, c DNA synthesis, proportion, construction uses. CDNAlibrary Vs genomic DNA library. Sequencing; Maxim Gilbert sequencing, Sanger sequencing,Shortgun approach.

Module - IVApplication of r DNA technologies: Nucleic acid sequences as diagnostic tools.

Applications in SAGE, EST, FISH, Micro array, Restriction mapping, blot analysis southern,Northern and western blot. PCR and its applications. Molecular markers: RFLP, RAPD, AFLP,

gene cloning in medicine (insulin Blood clotting factor VIII) High level.


Old RW, Primose SB Principles of gene manipulation-An introduction to geneticengineering. Blackwell Scientific Publications, 1993.

T.A.Brown. gene cloning.B.R.Glick & Jack J Pasternak Molecular Biotechnology.


47/129

BT 09 602BIOPROCESS ENGINEERING

Teaching Scheme:

3 hours lecture and 1 hour tutorial Credits: 4

Objectives:

To impart knowledge on the design analysis, monitoring, modelling and

simulation aspects of a bioreactor

To strengthen the knowledge of the design, operation and stability analysis of a

bioreactor
















48/129

Module - IIntroduction to fermentation process. Batch growth. Quantifying cell concentration Kinetics inbatch culture. Environmental conditions on growth. Monod model. Growth kinetic withplasmid instability. Product formation kinetics. Structured and unstructured models. Thermal

death kinetics of cell and Spores. Continuous culture. Monod chemostat model.

Module - II

Media design for fermentation Medium requirements and formulation. Antifoams.Medium optimization. Medium sterilization batch and continuous sterilization. Spiral heatexchanger. Sterilization of air. Inoculum preparation for industrial fermentation operation ofa typical aseptic aerobic fermentation process.

Module - III

The oxygen requirements of industrial fermentation. Oxygen supply. The determinationof KLa values . The sulphite oxidation method. Gassing out method. Factors affecting oxygentransfer rate in fermenters like bubble size, gas hold-up, gas velocity, temperature, pressure etc.

Power required for sparged and agitated ve

calicut Digree Syllabus And Scheme For Biotechnology

Documents

Transcript of calicut Digree Syllabus And Scheme For Biotechnology