By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results ...
-
Upload
nicholas-burke -
Category
Documents
-
view
215 -
download
0
Transcript of By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results ...
The Treatment and Prevention of Dental
CariesBy: Scott Karas
Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses
Overview
What are they?◦ Cavities
How are they formed?◦ Streptococci mutans
How to prevent them?◦ Brush your teeth◦ Vaccine?
Dental Caries
GTF- Glucosiltransferase GbpB- Glucan binding protein B CAT- Catalytic Domain
◦ GTF epitope GLU- Glucan binding domain SYI- peptide complex
◦ GbpB epitope WIS-Water insoluble glucan
Key Enzymes/Antibodies
Purpose of this experiment Bacteria used
◦ Streptococci mutans◦ Streptococci sobrinus
Synthetic peptides Three experiments
◦ 24-37 day old rats Measured the levels of antibodies Measured dental caries
Experiment Overview
Peptides◦ Three monoepitopic◦ Two diepitopic
Proteins◦ GbpB from S. mutans◦ GTP from S. sobrinus
ELISA◦ Measured IgA and IgG antibody levels
Methods
Experiment 1◦ 40 female rats◦ 6 groups of 7◦ Injected subconsciously
Experiment 2- S. mutans◦ 48 female rats◦ 4 groups of 12 in a cage◦ Injected subconsciously◦ Asphyxiated
Cleaned with beetles and ethanol
Methods
Experiment 3- S. sobrinus◦ Identical to Experiment 2◦ Streptomycin-resistant S. sobrinus
Bacterial Recovery◦ ELISA◦ Cotton swab
Carie assessment
Methods
Human Trials◦ Put in cows milk or eggs
Treatment for children or infants◦ “Window of Infectivity”
Treat young mothers◦ Breast feeding
Potential Uses
Dental Caries Methods Results Potential Uses
Summary
Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses
Summary
Smith, Daniel J., William F. King, Joy Rivero, and Martin A. Taubman. "Immunological and Protective Effects of Diepitopic Subunit Dental Caries Vaccines." Infection and Immunity 73.5 (2005): 2797-804. Print.
Russell, Michael W., Noel K. Childers, Suzanne M. Michalek, Daniel J. Smith, and Martin A. Taubman. "A Caries Vaccine?" ProQuest Nursing & Allied Health Source 38.3 (2004): 230-35. Print.
References