By Dominic Lanni. Part One Introduction What are biosensors? A device that uses a living organism...
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Transcript of By Dominic Lanni. Part One Introduction What are biosensors? A device that uses a living organism...
![Page 1: By Dominic Lanni. Part One Introduction What are biosensors? A device that uses a living organism or biological molecule to detect things Usually.](https://reader036.fdocuments.in/reader036/viewer/2022070400/56649f0d5503460f94c21e53/html5/thumbnails/1.jpg)
BIOSENSORSBy Dominic Lanni
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Overview of Biosensors
Part One
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Introduction
What are biosensors? A device that uses a living organism or
biological molecule to detect things Usually chemicals, heavy metals,
pathogens
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Famous Person
Pioneer of technology is Anthony P. F. Turner
Introduced screen-printing techniques
Developed blood-glucose sensors for diabetics
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Types of Biosensors
Can be Electrochemical
(Amperometry)Electrical (Conductivity)Optical (Fluorescence)Mass Sensitive
(Piezoelectric frequency)Thermal (Heat of
reaction/adsorption)
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Design ProposalPart Two
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Purpose
Dysentery-not fun (duh) Caused by an amoeba or a
bacterium called Shigella No vaccine available
(although there are treatments)
Proposal: a Mass-Sensitive BiosensorWill detect bacteria in water
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Competing Technologies
Conventional bacterial cultures
Immunological methods
Molecular microbiological methods
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The Design Mass-sensitive
Utilizes piezoelectric quartz crystal
DNA probe attached to crystal
Water sample put through DNA isolation process, and heated to denature strandsPCR may also be performed
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The Design DNA is pipetted in, and
heated to 50 degrees Celsius
Strands anneal to probe, increasing mass of crystal
Change in mass creates change in frequency
Change in frequency signals presence of Shigella
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Expected Results
If the frequency emitted by the crystal changes, then DNA has bound to probe
If DNA binds to probe, then Shigella bacteria are present in water sample
Knowledge of contamination prevents spreading and contraction of dysentery
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Advantages
No lack of supplies, as in bacterial cultures
Can be extremely fast Crystals are cheap
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Potential Problems Could be difficult to assemble on a large scale PCR would slow down process if necessary for
success Portable power source for whole system necessary, as
finding an outlet may be difficult Crystals can degrade in quality over time
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Testing
Tests to determine how accurate device is
Tests to determine if PCR is necessary or not
Environmental tests to determine hardiness of device and crystals
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THIS CONCLUDES THE
PRESENTATIONHope you learned something.
I don’t care what.
Anything is great.