Kotak Securities- Investment Strategist- Unzipping Growth - June 2014
Buffers used in dna overstretching and unzipping experiments
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Transcript of Buffers used in dna overstretching and unzipping experiments
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Buffers used in DNA-overstretching and unzipping experiments
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• Popping Buffer
• PBS
We primarily use popping buffer and PBS for DNA-unzipping and overstretching experiments
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Popping Buffer
Chemicals used in popping buffer:• EDTA:
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• Sodium Phosphate• Monobasic:
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• Dibasic
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• NaCl
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• Tween20:
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• Water H2O:
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• D2O
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Quantities and measurements
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CalculationsLabView
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Weight & Measure
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Store
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PBS
Chemicals used in PBS: PBS and H2O or D2O
• Dissolve 1 table in 200mL of H2O or D2O
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BGBBlotting Grade Blocker
To make 5mg/ml BGB solution in popping buffer:•First wiight out 15mg of BGB poweder•Add 1.5 ml of 1x pop and mix by vortexing•Run through .2μM syringe filter using 3ml syringe.•Now store 5mg/ml BGB in 1x pop buffer (minus amount of protein that stuck to filter) at 3C.This should be good for few weeks.
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Filter
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Anti-Dig
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Microspheres
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DNA