BSR SampleRail - chem.uic.edu · The information in this manual may be altered without notice....

BSR SampleRail

BSRUser Manual

Version
002

The information in this manual may be altered without notice.

BRUKER BIOSPIN accepts no responsibility for actions takenas a result of use of this manual. BRUKER BIOSPIN acceptsno liability for any mistakes contained in the manual, leading tocoincidental damage, whether during installation or operation ofthe instrument. Unauthorized reproduction of manual contents,without written permission from the publishers, or translationinto another language, either in full or in part, is forbidden.

This manual was written by

Klaus Ruf

© June 30, 2005: Bruker Biospin GmbH

Rheinstetten, Germany

P/N: Z31571DWG-Nr.: 1283002

User Manual

(Signature)

DECLARATION OF CONFORMITYThe under mentioned product(s)

BSR SampleRailP/N H9617

BSR Sample Rail VAC LeftP/N H10175

BSR Sample Rail VAC RightP/N H10192

conform(s) to the main requirements set by the commission forthe Harmonization of Regulations of the EU Member States

with regards to electromagnetic compatibility regulations(EMI: 89/336/ECC), safety regulations (Low Voltage Electrical

Equipment: 72/23/ECC), and machinery directives (98/37/ECC).

For the assessment the following norms were applied:

EMI: Emission: EN 61326:1997/A1:1998 / Class A;Immunity: EN 61326:1997/A1:1998 / EMC controlled area

Safety: IEC 61010-1. 2ed. 2001-02

Machinery Directives: DIN EN ISO 12100 - 1 & 2: 2004

Test report, NEMKO: CB Test Certificate NO30790Documentation: - Docu Standard: BSR SampleRail

Manufacturer’s Name: BRUKER BIOSPIN GMBHManufacturer’s Address: Silberstreifen 4,

D-76287 Rheinstetten,Germany

Declaration approved by:

Dr. Tonio Gianotti

Head of Development

Rheinstetten March 9, 2005

Version 002 BRUKER BIOSPIN 3 (107)

4 (107) BRUKER BIOSPIN User Manual Version 002

Contents

Contents ............................................................... 5

1 Introduction ........................................................... 91.1 Bruker SampleRail ............................................................... 91.2 About This Manual ............................................................. 101.3 Trademarks ....................................................................... 101.4 Warnings and Notes .......................................................... 101.5 Contact for Additional Technical Assistance ....................... 11

2 Safety Considerations ......................................... 132.1 Disclaimer ......................................................................... 132.2 Important Safety Considerations ........................................ 132.3 Correct Usage of SampleRail ............................................. 14

Personnel Safety ...........................................................14Technically Qualified Personnel Only .....................14

Hazards from Samples, Breakage of Glass... and Other Sub-stances ..........................................................................15Availability of Information ...............................................15Before the First Start ......................................................15Activities Before Each Start ............................................16Normal Operation ..........................................................16Maintenance ..................................................................16Cleaning ........................................................................17Replacement Parts ........................................................17Installation, Relocation and Disposal of the Device ........18

2.4 Customer Responsibilities ................................................. 182.5 Potentially Hazardous Areas .............................................. 19

Moving parts ..................................................................19Electrical parts ...............................................................20

3 System Description ............................................. 213.1 The SampleRail System .................................................... 21

Horizontal and Vertical Units ..................................21The SampleRail/Robot Transfer Unit ......................21The Transfer Unit Vertical/Horizontal ......................22The SampleRail/Magnet Transfer Unit ....................22Sample Trolley .......................................................22Fixing and Mounting Parts ......................................22SampleRail Control and Pneumatic Unit .................22

3.2 Bruker SampleRail VAC ..................................................... 243.3 Where to Find the Type Label ............................................ 25

User Manual Version 002 BRUKER BIOSPIN 5

Contents

4 Installation Instructions .......................................274.1 General ............................................................................. 274.2 Mounting the Horizontal Arm Above the Magnet ................ 274.3 Pneumatic Connections ..................................................... 284.4 Electrical Connections ....................................................... 314.5 Mounting the BSR Flexible Tube ....................................... 34

Assembling the BSR Flexible Tube ....................... 354.6 Mounting the BSR VAC Flexible Tube ................................ 38

Assembling the BSR VAC Flexible Tube ................ 39

5 Settings and Adjustments ....................................415.1 General ............................................................................. 415.2 Adjustment of the Sample Lift Air Flow (BSR only) ............ 415.3 Adjustment of the Buffer Air for the Sample Lift (BSR only) 425.4 Adjustment of the Suction Air for the BSR VAC Sample Lift 425.5 Adjusting the BSR VAC Temperate Air Flow ...................... 435.6 Adjusting the Sample Down Unit for SampleRail ................ 435.7 Adjusting the Sample Down Detector for BSR VAC ............ 45

6 Operating Instructions .........................................476.1 Front Panel Controls ......................................................... 476.2 Starting the System ........................................................... 476.3 Automatic Mode ................................................................ 486.4 Manual Insertion Mode ...................................................... 48

Switching to Manual Insertion Mode .............................. 48Manual Insert ................................................................ 49Manual Eject ................................................................. 49

6.5 The Occurrence of a Failure .............................................. 50

7 Software Commands ............................................517.1 SampleRailTM Command Implementation ........................... 517.2 Operation Mode ................................................................ 52

Standard Commands ............................................. 527.3 Error Mode ........................................................................ 597.4 Diagnostic Mode ............................................................... 60

8 Communication with the Tecan Robot .................698.1 Introduction ....................................................................... 698.2 Commands ....................................................................... 69

9 Error Handling ......................................................739.1 Introduction ....................................................................... 739.2 Error Messages ................................................................. 73

Errors Requiring User Intervention ................................. 73Communication Errors ................................................... 76

10 Operator Maintenance ..........................................7710.1 Introduction ....................................................................... 77

6 BRUKER BIOSPIN User Manual Version 002

Contents

10.2 Cleaning ............................................................................ 7710.3 Greasing the Pneumatic Linear Drives ............................... 7810.4 Cleaning After an NMR-Tube Breaks ................................. 7910.5 Pivoting the SampleRail Horizontal Arm ............................. 7910.6 Pivoting the BSR ............................................................... 81

Pivoting the BSR VAC ....................................................8310.7 Opening the BSR Cover .................................................... 85

Removing the BSR Covers .............................................85Opening of the BSR VAC Hood ......................................86

11 Barcode Reader Option ....................................... 8911.1 Introduction ....................................................................... 89

Operation without a Barcode Reader ......................90Operation with a Barcode Reader ..........................90

11.2 The CCD Barcode Reader ................................................. 9011.3 The 2D Barcode Reader .................................................... 91

12 Standard Accessories ......................................... 9312.1 BSR Accessories ............................................................... 93

Sample Accessories ......................................................93Tool Accessories ............................................................94

12.2 BSR VAC Accessories ....................................................... 95Sample Accessories ......................................................95Tools Accessories ..........................................................96

13 Technical Data ..................................................... 9713.1 Power Supply Requirements .............................................. 9713.2 Fuse Protection ................................................................. 9713.3 Compressed Air Requirements .......................................... 9713.4 Serial Communication ........................................................ 9713.5 Noise Level ....................................................................... 9813.6 Environmental Requirements ............................................. 98

Figures ................................................................ 99

Tables ................................................................. 101

Index .................................................................. 103

User Manual Version 002 BRUKER BIOSPIN 7

Contents

8 BRUKER BIOSPIN User Manual Version 002

1Introduction 1

Bruker SampleRail 1.1

The SampleRail™ is part of a system, under the management of the Sample-Track™ Laboratory Information Management System (LIMS) and ICONNMR™automation software, that provides automatic preparation and mixing of multiplecompounds, and subsequent NMR measurements.

A typical application example is the protein-ligand binding detection by NMR. Thissystem automatically prepares an NMR sample, inserts it into an NMR magnet,performs NMR experiments on the sample, and transports it back to the prepara-tion system.

The SampleRail fulfills the transporting tasks from the preparation system into theNMR magnet and back, while a TECAN™ Genesis/Freedom pipetting robot isused as the preparation system. The SampleRail may also be used in a "manualinsertion mode" to insert and eject single NMR samples manually into the NMRmagnets.

SampleRail may also be used in a ’manual insertion mode’ to insert / eject singleNMR samples manually into the NMR magnet.

Figure 1.1. The BRUKER SampleRail and the TECAN Robot

User Manual Version 002 BRUKER BIOSPIN 9 (107)

Introduction

About This Manual 1.2

This manual is included with the delivery of the SampleRailTM system and pro-vides instructions on how to:

• install and configure the SampleRail system;

• operate the system.

This manual applies to the SampleRail basic version - from this point on referredto as the BSR. It also applies to the SampleRail system with a vacuum unit, whichis available in either left or right orientated.

• BSR = Bruker SampleRail

• BSR VAC = Bruker SampleRail VAC left / right

The term SampleRail includes all the above mentioned versions.

Trademarks 1.3

All product names and marks mentioned in this document are trademarks or reg-istered trademarks and property of their respective owners.

SampleRailTM is a trademark of Bruker BioSpin GmbH.

TECAN product names are trademarked products or registered trademarks ofTECAN Group Ltd.

Warnings and Notes 1.4

There are two types of information notices used in this manual. These noticeshighlight important information or warn the user of a potentially dangerous situa-tion. The following notices will have the same level of importance throughout thismanual.

Note: Indicates important information or helpful hints

WARNING: Indicates the possibility of severe personal injury, loss of life orequipment damage if the instructions are not followed.


Contact for Additional Technical Assistance

Contact for Additional Technical Assistance 1.5

For further technical assistance on the SampleRail system, please do not hesitateto contact your nearest BRUKER dealer or contact us directly at:

BRUKER BIOSPIN GMBHSilberstreifenD-76287 RheinstettenGermany

Phone: + 49 721 5161 0FAX: + 49 721 5171 01E-mail: [email protected]: www.bruker-biospin.de/nmr/


Introduction


2Safety Considerations 2

Read these safety instructions carefully and make them accessible to anyoneworking around the SampleRail system. A SampleRail system can be operatedeasily and safely provided the correct procedures are followed and certain pre-cautions observed.

Disclaimer 2.1

Bruker is not responsible or liable for any injury or damage that occurs as a con-sequence of non-approved manipulations on the SampleRail system.

Important Safety Considerations 2.2

• The power supply voltage must correspond to the information listed in "PowerSupply Requirements" on page 97.

• The compressed air supply must correspond to the specifications listed in"Compressed Air Requirements" on page 97.

• Never touch any moving parts, always wait until the device has come to a com-plete stop.

• Please note that in automation mode the device may restart unexpectedly.

• In case of malfunction during operation, stop the device, disconnect the powersupply and inform customer service.

• Always use original manufacturer accessories.

• Do not use the device if the power cord, compressed air hose, or the device it-self have visible damage.

• Inappropriate or improper repairs may result in personal injury to the user.Never disassemble the device or try to repair it. Repairs and disassemblyshould only be carried out by experienced technicians.


Safety Considerations

Correct Usage of SampleRail 2.3

Operational safety can only be guaranteed if SampleRail is used properly. Sam-pleRail offers two modes of operation:

Appropriate usage of the device includes reading this manual and observing allrecommendations - especially all safety aspects.

All service and routine maintenance must be performed within the prescribed timeintervals as outlined in "Operator Maintenance" on page 77. Failure to do so willbe considered improper use of the product and will invalidate any warranties.

If the SampleRail is used in any way other than for what it was intended, as out-lined in this manual, this will constitute ’unauthorized use’. Bruker is not responsi-ble or liable for any injury or damage that occurs as a consequence of improper orunauthorized usage of the SampleRail system.

Personnel Safety 2.3.1

The SampleRail system may only be operated by trained and authorized person-nel. All personnel who work with, or in the close proximity of a SampleRail system,need to be informed of all safety issues and emergency procedures as outlined inthis user manual.

Technically Qualified Personnel OnlyThe transportation, mounting, start-up, instruction, maintenance, cleaning, ser-vice, repair and shutdown of the SampleRail system should only be carried out bytrained, qualified and authorized personnel.

New users must be supervised by experienced personnel! It is highly recommend-ed that a company training program be implemented for training new users on allaspects of product safety and operation.

Manual Insertion Mode: Manual insertion/ejection of NMR samples is con-trolled by the front panel buttons of the SampleRail control unit.

Automatic Mode Automatic insertion/ejection of NMR samples is remotely controlled by the spectrometer host com-puter, as described in the chapter "Operating Instructions" on page 47.


Correct Usage of SampleRail

Hazards from Samples, Breakage of Glass... and Other Substances 2.3.2

• When operating SampleRail using glass tubes, there is always a dangerpresent when using samples with chemical or biological substances and sol-vents.

• Always use undamaged glass tubes. Damaged glass tubes need to be ex-changed immediately.

• When operating the BSR VAC only use 4.25x100mm glass tubes with the cor-responding sample holder (tube, septum, barcode label).

• The user’s organization is responsible for taking measures to avoid inherentrisks in the handling of dangerous substances, preventing industrial disease,and providing medical first aid in emergencies.

• The user’s organization is responsible in providing facilities and making dispo-sition according to the local regulations for the prevention of industrial acci-dents and generally accepted safety regulations according to the rules ofoccupational medicine.

• All substances needed for operating and cleaning the device - samples, sol-vents, cleaning agents, gases, etc. have to be handled with care and disposedof appropriately. All hints and warnings on storage containers must be readand adhered to!

• The use of glass tubes may result in cuts and stab wounds if a tube should bedamaged. Therefore, appropriate care and diligence must be observed whenusing these devices.

Availability of Information 2.3.3

This SampleRail user manual must be kept with the device. All personnel operat-ing the device must have access to the SampleRail user manual at all times.

In addition to the SampleRail user manual, instructions concerning labor protec-tion laws, operator regulations tools and supplies must available and be adheredto.

All warning and user information labels must be kept in a good and fully readablestatus. Unreadable or damaged labels should be replaced immediately.

The following markings on the SampleRail must be observed:

• Warning symbols,

• Labeling for electric and compressed air connections.

These markings should always be maintained in a fully readable condition.

Before the First Start 2.3.4

Familiarize yourself with:

• The operating and control elements.

• The instrumentation.

• The mode of device operation.



• The device’s environment.

• The safety arrangements of the device „Opening the BSR Cover“ onpage 85.

• All emergency procedures (operating company).

Activities Before Each Start 2.3.5

Before every start the following activities are to be executed:

• Examine and ensure that all security devices (hoods/enclosures) are appropri-ate and in working condition.

• Visible observe the device for any obvious damage; observed defects have tobe eliminated immediately and/or reported immediately to the responsible par-ty - SampleRail should only be used when in fully operational condition.

• Check and ensure that only authorized personnel remain in the work area dur-ing operation and that no one is at risk when the device is started.

• All objects and materials that are not required for the operation mode shouldbe removed from the work area.

Normal Operation 2.3.6

During operation no security devices should be removed or switched off.

The user must observe that no unauthorized people remain in the SampleRailwork area.

During operation of the device in Normal Mode the following special haz-ards can arise:

• Inappropriate use of the device in combination with dangerous or infectioussamples and solvents may result in a life-threatening situation. Laboratory andgeneral safety instructions (operating company) for the care, handling anduse of substances and samples have to be observed without restriction at alltimes.

• Inappropriate reaction to hazardous incidents may result in personal injury anddamage to property - be familiar with the company regulations on handlinghazardous incidents.

Maintenance 2.3.7

Before and during the service maintenance the following rules have to beconsidered:

• Switch off the electrical power supply on the back of the BSR Control Unit.

• Operating personnel must observe that no unauthorized people remain in theSampleRail work area.

• Change faulty parts of the device immediately.

• Use only original manufacturer spare parts.


Correct Usage of SampleRail

• Storage tanks must be available for substances that might cause ground waterpollution.

After the service maintenance and before a new start of the device the fol-lowing rules have to be considered:

• Verify that all screws/bolt connections which were loosened have been retight-ened correctly.

• Check, that all protection devices and hoods have been replaced properly.

• Verify that tools, materials and other equipment used have been removed fromthe work area.

• Clean the work area and remove any leaking fluids and lubricants.

• Verify that all protection devices are working correctly.

Detailed maintenance guidelines for each of the individual components of Sam-pleRail within this manual should also be adhered to.

Cleaning 2.3.8

Use only water or mild neutral cleaning agents for cleaning. Usage of a diluter orgasoline will damage the surface of the device.

When cleaning the device the following guidelines must be followed:

• Switch off the electrical power supply on the back of the BSR Control Unit andunplug the mains cable before cleaning.

• Clean the device with a dry or moist cloth.

• Dry the device completely before plugging in the mains cable.

Replacement Parts 2.3.9

For safety reasons the device must not be modified without pre-authorization inwriting from Bruker.

Use only Bruker original spare parts and accessories. These parts are especiallydesigned for the device.

Parts and equipment, that are not supplied by Bruker, are not authorized for use,and no guarantee is given for the compatibility and safety of these products.

Bruker takes no responsibility for consequences arising from the use of unautho-rized parts and equipment.



Installation, Relocation and Disposal of the Device 2.3.10

The installation, replacement, and relocation of SampleRail should only be carriedout by authorized Bruker personnel.

Consult Bruker customer service for information on the disposition of SampleRail.

Customer Responsibilities 2.4

SampleRail was constructed and built state-of-the-art based on a complete safetyanalysis and harmonized standards.

The proven level of safety can only be obtained when the operator takes reason-able precautions. It is the operator’s due diligence to make all necessary safetyprecautions and continuously control them.

The operating company has to ensure that:

• SampleRail is used only in accordance with established regulations.

• The device is used only in proper operational condition and that the safetymeasures are checked at regular intervals.

• All warning notices and safety instructions attached to the device, stay read-able.

• The operational manual stays in a readable state and is available at the usagesite.

• Only qualified and authorized personnel operate, maintain and repair the de-vice.

• All personnel are instructed regularly in all applicable industrial safety and en-vironmental protection standards, as well as the information contained withinthis manual, especially all references to safety.

• All necessary safety equipment for operation, maintenance, and repair areavailable and are utilized.

• A safety evaluation be routinely conducted to further identify whether any localor special conditions exist that may effect the working conditions and/or safetyof users.

• The results of the safety evaluation be summarized and posted in the workarea to inform personnel of potential risks.


Potentially Hazardous Areas

Potentially Hazardous Areas 2.5

Moving parts 2.5.1

SampleRail is driven by compressed air, even with control unit turned off, therecan still be pressure in the cylinders. Thus unexpected movement of the trolleyis possible.

During operation avoid putting hands or objects into the moving parts ofthe SampleRail, as this may cause personal injury or damage to the equip-ment.

The BSR is covered with a hood at the transfer unit on the robot side and at themagnet to prevent damage to the BSR and to prevent personal injury.

The BSR VAC is covered with an enclosure which protects the entire path of thetrolley.

Do not open the hood/enclosure when SampleRail in operating. Opening of thehood/enclosure does not interrupt the trolley movement.

Repair work that requires a trolley movement with the hood/enclosure opened,should be done only by authorized technical personnel.

The following warning symbol is located on the hoods/enclosures:

Figure 2.1. Warning Symbol at the Hood/Enclosure of SampleRail



Alternatively the following warning symbol may be appropriate at the accesspoints of the SampleRail trolley:

Figure 2.2. Alternative Warning Symbol on SampleRail

Electrical parts 2.5.2

The SampleRail control unit is driven by line voltage. If the control unit has to beopened for service or repair disconnect the mains cable before opening.

Inside the SampleRail control unit live parts are not covered. Coming incontact with them may result in personal injury!

Remember that all service must be done by trained service personnel only.

The following warning symbol is found at the rear panel of the SampleRail controlunit:

Figure 2.3. Warning Label at the Rear Panel of the SampleRail Controller


3System Description3

The SampleRail System 3.1

The SampleRail system consists of the subassemblies shown in the figure below:

Figure 3.1. The Bruker SampleRail System

Horizontal and Vertical UnitsThe motion of the SampleRail trolley is provided through pneumatic cylinderswhich make up the main parts of the horizontal and the vertical units.

The length of the horizontal and vertical units may vary according to the individualneeds in the customers laboratory layout.

The SampleRail/Robot Transfer Unit The TECAN® pipetting robot places/removes prepared samples from the insert/eject position of the trolley at the SampleRail/robot transfer unit.

Horizontal Unit

Vertical Unit

Flexible TubeTransfer Unit

Hood

Transfer UnitNMR Magnet

Robot Table

Pipetting RobotTECAN

Transfer Unit

SampleRail / Magnet

SampleRail / Robot

Vertical / HorizontalHood

Mounting PartsTrolley


System Description

The Transfer Unit Vertical/HorizontalThe sample is passed from the horizontal unit to the vertical unit trolley (and viceversa) at the transfer unit vertical/horizontal.

The SampleRail/Magnet Transfer Unit SampleRail is connected to the shim upper part of the NMR magnet from theSampleRail/magnet transfer unit via a flexible tube. The samples may be insertedinto or ejected out of the magnet pneumatically through this tube. The tube’s flexi-bility guarantees the decoupling of the magnet and the SampleRail, which helpsprevent interference from mechanical oscillations.

Sample TrolleyThe sample trolley transports the samples and consists of two positions for thesample tubes:

• the insert position for new samples (marked with an ’I’);

• the eject position for pre-measured samples (marked with an ’E’).

The advantage of having two positions is that a sample exchange can be complet-ed without a major loss of time.

Fixing and Mounting PartsThe various parts which are needed to fix the horizontal and vertical units dependon the individual laboratory and building layout, and thus are customer specific.

SampleRail Control and Pneumatic UnitThe SampleRail control unit and the pneumatic unit are both 19“ racks which aremounted in the cabinet of the TECAN® robot. These units control SampleRail andprovide communication between the TECAN® and the spectrometer, where themain automation management software runs.


The SampleRail System

Figure 3.2. SampleRail Communication Block Diagram

Figure 3.3. Example of a SampleRail System

Spectrometer. SampleRail

TECAN

Sensor

Controller

TECANI/O Box

TECANRobot

Controller

Distributionat Magnet

SensorDistribution

at X/Y Crossing

SensorDistribution

at TECAN Robot

LAN

RS232

RS232 RS232 Barcode Reader(optional)

RS

232

RS

232

sensor signals

sensor signals

sensor signals

valv

e si

gnal

s

SampleRailPneumatic Unit

pneumaticconnectionsto cylinders

Host

SampleTrack

TECAN

SampleRail

Computer


System Description

Bruker SampleRail VAC 3.2

The BSR VAC is a customized SampleRail system. It allows the handling of spe-cial sample holders, whereas the tube is closed with a septum and is providedwith a barcode label. An optimized TECAN® Freedom robot is integrated into thesystem.

As opposed to the normal BSR, the BSR VAC draws the measured sample intothe trolley/carrier with the help of a vacuum unit, rather than blowing it out of themagnet.

Communication between the BSR control unit, spectrometer host computer, andTECAN® robot is based on the same command language as with BSR.

The basic differences between the BSR and the BSR VAC are that the BSR VACsystem contains:

• A new sample trolley adapted for use with the new sample holder.

• A vacuum unit is located within the transfer unit above the magnet.

• A special shim upper part is used.

• A new sample detection unit is used to detect the sample in the magnet.

• A hood is used to seal the entire transport system.

Figure 3.4. Example of a BSR VAC System


Where to Find the Type Label

Where to Find the Type Label 3.3

You will find the SampleRail type label on the lower end of the vertical linear drive.

Figure 3.5. The BSR SampleRail Type Label

The SampleRail controller type label is attached to the back panel.

Figure 3.6. The BSR Control Unit Type Label

The Pneumatic Unit type label is also attached to the back panel.

Figure 3.7. BSR Pneumatic Unit Type Label

Manufacturer

Part number Serial number

Type

Manufacturer

Part number

Serial number

Type

Electrical data

Manufacturer

Part number

Serial number

Type

Air input data


System Description


4Installation Instructions 4

General 4.1

SampleRailTM can only be installed by trained BRUKER service personnel. A fewinstallation examples are described in this chapter to illustrate typical installationscenarios.

Mounting the Horizontal Arm Above the Magnet 4.2

Because of the transmission of vibrations to the magnet, no fixed support of theSampleRail horizontal arm to the magnet is possible. Therefore, to support theweight of the horizontal arm, normally it is necessary to fasten it to the ceiling. Themechanical parts and their fixation are planned by BRUKER based on the sites in-dividual requirements.

The structural requirements and fixation of these parts at the building site are theresponsibility of the customer.

Figure 4.1. Attaching the Horizontal Arm


Installation Instructions

Pneumatic Connections 4.3

Figure 4.2. Pneumatic Unit Rear Panel

The customer is responsible in providing a compressed air connection with a sep-arator and a compression delimiter unit according to EN983. The maximum airpressure is 8 bar or 116 pound-force/square inch (PSI). See "Compressed AirRequirements" on page 97).

The system must be depressurized prior to disconnecting hoses, which will pre-vent hose backlash.

VALVES

0

bar

+

-

10 2 3 4 5 6

LIFTOUT

OUTVERTICAL

OUTHORIZONTAL

AIR IN5-7bar abs. max. 8bar

1

23

4

5

6

SPAREOUT

+-

SAMPLE ATT

PRESSURE REGULATOROut Pressure 0.5bar to PrIN

VALVE RESSURE OUT

ManometerConnector toSampleRail Controller Pressure Reducer

Pneumatic HoseConnector Assembly Pneumatic Hose Connector

to Sample Lift

Pneumatic Hose ConnectorSupply of Vertical Cylinder

Pneumatic Hose ConnectorSupply of Horizontal Cylinder

Pneumatic Hose ConnectorMain Supply Air In(Connectors Number-coded)

Pneumatic Hose ConnectorSpare

Pneumatic Valve Buffer Air


Pneumatic Connections

Figure 4.3. BSR Pneumatic Connections

VALV

ES

0

bar

+

-

10

23

45

6

LIFT

OU

T

OU

TVE

RTI

CA

L

OU

TH

OR

IZO

NTA

L

AIR

IN5-

7bar

abs

. max

. 8ba

r

1

23

4

5

6

SPA

RE

OU

T

+

-SAM

PLE

ATT

PRES

SUR

E R

EGU

LATO

RO

ut P

ress

ure

0.5b

ar to

PrIN

VALV

E R

ESSU

RE

OU

T

13

2

13

2

13

2

13

2

Mag

net

from

BSM

SLI

FTO

UT

Air S

uppl

ym

in. 5

bar

max

6ba

r

Pneu

mat

ic C

ontro

l Uni

t Rea

r Pan

el

Hor

izon

tal C

ylin

der

Ends

top

Cyl

inde

r

Out

let

Grip

per

3/2

Valv

e

Out

let

Bolt

Cyl

inde

r

Verti

cal

Cho

ke

Cho

ke

Cho

ke

Cyl

inde

r(w

hite

)

Cyl

inde

r

BSR

- PN

EUM

ATIC

CO

NN

ECTI

ON

SPn

eum

atic

_BSR

.fm

Out

let

Cho

ke

Out

let

Cho

ke



Figure 4.4. Pneumatic Connections BSR VAC

VALV

ES

0ba

r

+

-

10

23

45

6

LIFT

OU

T

OU

TVE

RTI

CA

L

OU

TH

OR

IZO

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L

AIR

IN5-

7bar

abs

. max

. 8ba

r

1

23

4

5

6

SPA

RE

OU

T

+

-SAM

PLE

ATT

PRES

SUR

E R

EGU

LATO

RO

ut P

ress

ure

0.5b

ar to

PrIN

VALV

E R

ESSU

RE

OU

T

13

2

13

2

13

2

13

2

Mag

net

Air

Sup

ply

min

. 6ba

rm

ax 7

bar

Pne

umat

ic C

ontro

l Uni

t Rea

r Pan

el

Hor

izon

tal C

ylin

der

End

stop

Cyl

inde

r

Out

let

Grip

per

3/2

Val

ve

Out

let

Bol

t Cyl

inde

r

Ver

tical

Cho

ke

Cho

ke

Cyl

inde

r

Cyl

inde

r

13

4 52

5/3

Val

ve

Suc

tion

Tube

Cyl

inde

r

13

21

32

Vac

uum

Pum

p

BS

R V

AC

- PN

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MA

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NS

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BW

.fm

Out

let

Cho

ke

Out

let

Cho

ke


Electrical Connections

Electrical Connections 4.4

Figure 4.5. Control Unit Rear Panel

SENSOR3

SENSOR2

SENSOR1

DIAGNOSTICVALVES TECAN BARCODE

SERVICE

HOST

FUSE2x1AT

Connectors to sensor

Connector to SIO to spectrometer

Connector for optionalMain supply switch

SIO to TECAN robot

Main supply plug

barcode reader

host computerpneumatic unitfor valves control

distribution boards

with fuses



Figure 4.6. Electric Connections BSR

VALV

ES

0ba

r

+

-

10

23

45

6

LIFT

OU

T

OU

TVE

RTI

CA

L

OU

TH

OR

IZO

NTA

L

AIR

IN5-

7bar

abs

. max

. 8ba

r

1

23

4

5

6

SPA

RE

OU

T

+

-SAM

PLE

ATT

PRES

SUR

E R

EGU

LATO

RO

ut P

ress

ure

0.5b

ar to

PrIN

VALV

E R

ESSU

RE

OU

T

SEN

SOR

3

SEN

SOR

2

SEN

SOR

1

DIA

GN

OST

ICVA

LVES

TEC

AN

BA

RC

OD

E

SER

VIC

E

HO

ST

FUSE

2x1A

T

P/N

HZ1

0151

P/N

HZ1

0153

P/N

667

50

RS

232

Cab

le to

Spe

ctro

met

er H

ost C

ompu

ter T

TY

RS

232

Cab

le to

TEC

AN

Per

sona

l Com

pute

r CO

M1

Line

Cor

d

X2

X3

X4

X5

X6

X7

X11

X10

X8

X12

X1

X1

X2

X3

X4

X5

X6

X7

X1

X2

X3

X4

X5

X6

X7

P/N

HZ1

0152

Sen

sor D

istri

butio

nat

XY

-Cro

ssin

gS

enso

r Dis

tribu

tion

at M

agne

t

Sen

sor D

istri

butio

nat

Hom

e P

ositi

on

P/N

300

0

12

3

Ligh

t bar

rier

switc

h de

tect

or

switc

h de

tect

or

optic

al re

flect

ive

sens

or

carr

iage

abo

ve m

agne

t

grip

per c

ylin

der

grip

per o

pen

sam

ple

up

trolle

y ab

ove

mag

net

ends

top

cylin

der

Dis

tribu

tion

Box

at S

him

tube

(P/N

HZ0

7506

)

to B

SM

S

to c

onne

ctor

at

hood

clo

sed

horiz

onta

l car

riage

verti

cal

carr

iage

trolle

y at

sam

ple

in

sam

ple

in

carr

iage

at

hom

e po

sitio

n

ejec

t pos

ition

inse

rt po

sitio

n

hom

e po

sitio

n

shim

upp

er p

art

P/N

HZ0

7622

P/N

HZ0

7612

P/N

HZ0

7624

P/N

HZ0

7623

hood

clo

sed

P/N

HZ0

7630

P/N

HZ0

7628

ejec

t pos

.in

sert

pos.

P/N

HZ0

7627

activ

e P

/N H

Z076

29

P/N

HZ1

0177

P/N

HZ0

9162

P/N

HZ1

0189

P/N

HZ1

0157

P/N

Z12

152

P/N

100

43 (1

0m)

or P

/N H

Z335

1 (2

0m)

All

sens

or c

able

s at

the

dist

ribut

ion

boar

ds a

renu

mbe

r cod

ed

P/N

HZ0

7761

1

P/N

HZ0

7397

P/N

HZ0

7396

P/N

HZ0

7622

Sam

pleR

ail

Con

trol U

nit

(P/N

H95

77)

Pne

umat

ic U

nit

(P/N

H10

000)

Sam

pleR

ail

fiber

opt

ic s

enso

r

Sam

ple

Dow

n B

ox

BS

R -

ELE

CTR

ICA

L W

IRIN

Gw

iring

_BS

R.fm

P/N

HZ1

0192

Bar

code

Rea

der O

ptio

n

P/N

HZ0

4054

(2m

)

boar

d (P

/N H

9569

)


Electrical Connections

Figure 4.7. Electric Connections BSR VAC

SEN

SOR

3

SEN

SOR

2

SEN

SOR

1

DIA

GN

OS

TIC

VALV

ESTE

CA

NB

AR

CO

DE

SER

VIC

E

HO

ST

FUSE

2x1A

T

VALV

ES

0

bar

+

-

10

23

45

6

LIFT

OU

T

OU

TVE

RTI

CA

L

OU

TH

OR

IZO

NTA

L

AIR

IN5-

7bar

abs

. max

. 8ba

r

1

23

4

5

6

SPA

RE

OU

T

+

-SA

MPL

E A

TT

PRES

SUR

E R

EGU

LATO

RO

ut P

ress

ure

0.5b

ar to

PrIN

VALV

E R

ESS

UR

E O

UT

P/N

HZ1

0151

P/N

HZ1

0153

P/N

667

50

RS

232

Cab

le to

Spe

ctro

met

er H

ost C

ompu

ter T

TY

RS

232

Cab

le to

TEC

AN

Per

sona

l Com

pute

r CO

M1

Line

Cor

d

X2

X3

X4

X5

X6

X7

X11

X10

X8

X12

X1

X1

X2

X3

X4

X5

X6

X7

X1

X2

X3

X4

X5

X6

X7

P/N

HZ1

0152

Sen

sor D

istri

butio

nat

XY

-Cro

ssin

gS

enso

r Dis

tribu

tion

at M

agne

t

Sen

sor D

istri

butio

nat

Hom

e P

ositi

on

P/N

300

0

Ligh

t bar

rier b

oard

switc

h de

tect

or

switc

h de

tect

or

optic

al re

flect

ive

sens

or

carr

iage

abo

ve m

agne

t

grip

per c

ylin

der

grip

per o

pen

sam

ple

up

trolle

y ab

ove

mag

net

ends

top

cylin

der

horiz

onta

l car

riage

verti

cal

carr

iage

trolle

y at

sam

ple

in

sam

ple

in

carr

iage

at

hom

e po

sitio

n

ejec

t pos

ition

inse

rt po

sitio

n

hom

e po

sitio

n

P/N

HZ0

7622

P/N

HZ0

7624

P/N

HZ0

7630

P/N

HZ0

7628

ejec

t pos

.in

sert

pos.

P/N

HZ0

7627

activ

e P

/N H

Z076

29

P/N

HZ1

0177

P/N

HZ0

9162

P/N

HZ1

2829

P/N

100

43 (1

0m)

or P

/N H

Z335

1 (2

0m)

P/N

HZ0

4054

(2m

)

All

sens

or c

able

s at

the

dist

ribut

ion

boar

ds a

renu

mbe

r cod

ed

P/N

HZ0

7611

P/N

HZ1

2707

P/N

HZ1

2706

P/N

HZ0

7622

Sam

pleR

ail

Con

trol U

nit

(P/N

H95

77)

Pne

umat

ic U

nit

(P/N

H10

000)

Sam

pleR

ail

fiber

opt

ic s

enso

r

sam

ple

dow

n

P/N

HZ1

2684

AB

W D

etec

tion

Con

.su

ctio

n po

s.

suct

ion

pos.

off

Suc

tion

Tube

P/N

HZ1

2683

BS

R V

AC

- E

LEC

TRIC

AL

WIR

ING

wiri

ng_A

BW

.fm

P/N

868

22

Bar

code

Rea

der O

ptio

n

(P/N

H95

69)



Mounting the BSR Flexible Tube 4.5

The BSR flexible tube connection to the magnet is manufactured according to thelength required at the installation site. The quantity of the tube rings (P/NHZ07874) depends on the flexible tube’s length.

For optimal and safe sample transportation this tube must not be bent.

Figure 4.8. BSR Flexible Tube Components at the Shim Upper Part

conical ring(P/N HZ07335)

cap screw(P/N HZ07334)

tube rings(P/N HZ07874)



screws M3x20(P/N 11106)

adapter(P/N HZ07336)

toric joint(P/N 61667)

flexible tube(P/N HZ07322)

toric joint at transfer unitSampleRail/ magnet


Mounting the BSR Flexible Tube

Assembling the BSR Flexible Tube

1. Remove the ball head screws ontop of the shim upper part.

2. Fix the toric joint to the bottom of thetube adapter.

3. Put the adapter onto the top of theshim upper part.

4. Insert the adjusting device found inthe tool set with the standard acces-sories.



5. Fix the adapter with the M3x20screws and take out the adjustingdevice. The adjusting device shouldslide out easily.

6. Assemble the tube. Shift the tuberings onto it first, then the cap screwand at least the conical ring. Theconical ring has a bright edge and adark edge. The dark edge should beon the top.

7. Put the tube into the adapter.

8. Slide the conical ring downwards.


Mounting the BSR Flexible Tube

9. Fix the tube with the cap screw.

10.Arrange the tube rinds along thecomplete length of the tube.



Mounting the BSR VAC Flexible Tube 4.6

The BSR VAC flexible tube connection to the magnet is manufactured accordingto the length required at the installation site. The quantity of the tube rings (P/NHZ07874) depend on the length flexible tube’s.

To reach a safe sample transportation it must not be bent.

Figure 4.9. BSR VAC Flexible Tube Components at the Shim Upper Part



tube rings(P/N HZ12675)



screws M3x20(P/N 11106)

(P/N HZ12559)

toric joint at transfer unitBSR VAC / magnet

flexible tube 13x3

rubber collar(P/N 85360)

bearing case(P/N HZ12532)

HZ12530


Mounting the BSR VAC Flexible Tube

Assembling the BSR VAC Flexible Tube

1. Put the upper part of the tube ontothe adapter at the toric joint. Slidethe conical ring over the tube to-wards the adapter. Fix the tube withthe cap screw.

2. Slide the cap screw on the hosewith the large opening to the out-side. Slide the conical ring onto thetube. Place the lower part of thetube onto the adapter at the bearingcase. Slide the conical ring to theadapter. Fix the tube with the capscrew.

3. Assemble the rubber collar on thebearing case. Slide the rubber collarbottom-up over the bearing case.



4. Place the rubber collar on the shimupper part. For the BSR VAC a spe-cial shim upper part is used.

5. Slide the rubber collar top downover the shim upper part. Then ar-range the tube rings along the com-plete length of the tube.


5Settings and Adjustments 5

General 5.1

SampleRail settings and adjustments must be done by trained service personnel.Damages resulting from improper adjustments by untrained personnel are thecustomers responsibility.

Adjustment of the Sample Lift Air Flow (BSR only) 5.2

The valve to adjust the lift air flow is located at the top of the magnet.

• For faster sample lift turn the valve counter clockwise (open).

• For slower sample lift turn the valve clockwise (close).

Always adjust the settings in small steps and observe the effects each time duringa sample change before proceeding.

Figure 5.1. Valve to Adjust Lift Air Flow


Settings and Adjustments

Adjustment of the Buffer Air for the Sample Lift (BSR only) 5.3

The valve to adjust the air flow from the buffer tank, which is used to control thelift, is on the rear side of the BSR pneumatic unit.

• To lower the sample faster: Turn the valve in clockwise direction (close).

• To lower the sample slower: Turn the valve in the counter clockwise direction(open).


Figure 5.2. BSR Pneumatic Unit - Rear Side

Adjustment of the Suction Air for the BSR VAC Sample Lift 5.4

The strength of the low pressure used to pull the BSR VAC sample must be ad-justed using the input pressure of the BSR Pneumatic Unit.

The input pressure of the suction unit (BSR Pneumatic Unit - LIFT OUT) is not re-stricted by the BSR Pneumatic Unit valve.

The customer is responsible for provide a compressed air supply with an adjust-able pressure regulating valve for the BSR VAC.

The pressure needed here is 6,5 bar with an air consumption volume of about 100l/min. The pressure should be adjustable from 6 - 7 bar.

Adjust the pressure of the regulating valve at the installation site:

• For faster sample removal: Increase the pressure.

• For slower sample removal: Lower the pressure.



Adjusting the BSR VAC Temperate Air Flow

Adjusting the BSR VAC Temperate Air Flow 5.5

In order for the sample to optimally reach it’s measurement position in the magnetduring insertion (INSERT), the real temperate air flow rate should be a maximumof 350 l/h. To adjust this with XWIN-NMR or Topspin (edte) only the values 135 l/h or 270 l/h are permissible. We recommend selecting 270 l/h.

We also recommend that the real temperate air flow rate be checked by Brukerservice during the installation and when problems with the insertion of the sampleinto the magnet occur.

The air flow should be measured between the BVT module of the spectrometerand the probe using a flow meter. The measure value must agree with the valuesgiven in XWIN-NMR (edte).

An adjustment of the air flow can be made at the pressure control valve on theback of the BVT module if necessary (make calibration for 270 l/h).

Adjusting the Sample Down Unit for SampleRail 5.6

Two versions of the sample down unit have been used for SampleRail. These dif-fer in their housing color. The adjustment is carried out in connection with the ap-plied shim upper part. When replacing either the sample down unit or the shimupper part, the settings have to be readjusted. The adjustment range is differentfor both versions.

1. The BSR Sample Down Unit (P/N HZ07506 ECL 00) was used until 2003. Ithas a black housing. The adjustment range in connection with the applied shimupper part is not sufficient in certain cases. If necessary, this older unit can beexchanged for the newer BSR Sample Down Unit with ECL 01.

Figure 5.3. BSR Sample Down Unit ECL 00

2. Since 2004 the BSR Sample Down Unit (P/N HZ07506 ECL 01) with a whitehousing has been used.



Figure 5.4. BSR Sample Down Unit ECL 01

Figure 5.5. Adjusting the BSR Sample Down Unit

• The BSR Sample Down Unit (P/N HZ07506 ECL 01) is also used in conjunc-tion with the BSR VAC device. This unit verifies if a sample is in the magnet ornot. An extra adjustment is not necessary. The fine tuning adjustment is donewith the Fiber Amplifier Sample Down (HZ12829).

1. Remove the sample from the magnet.

2. Turn the adjustment thumb wheel clockwise until the orange sample down indicator LED is on.

3. Turn the adjustment thumb wheel slowly coun-terclockwise until the orange sample down indi-cator LED turns off. Turn two more turns upwards (counterclockwise).


Adjusting the Sample Down Detector for BSR VAC

Adjusting the Sample Down Detector for BSR VAC 5.7

Figure 5.6. Adjusting the Sample Down Fiber Amplifier

Two-point Calibration

1. Remove the sample from the magnet. Press the SET button and release it (The orange LED should light).

2. Position a sample in the magnet. Press the SET button again and release it.

3. The setting value is now adjusted to the midpoint of the difference in the received light intensity when the sample is absent and present.


6Operating Instructions 6

Front Panel Controls 6.1

The following controls are located on the front panel (see):

1. LIQUID CHRISTAL DISPLAY: Used to display the current status and errormessages.

2. RESET: A push button for system initialization.

3. CONT: A push button for continuation after a failure.

4. EJECT: A push button to start sample eject in manual insertion mode.

5. INSERT: A push button to start sample insertion in manual insertion mode.

Figure 6.1. The BSR Control Unit Front Panel

Starting the System 6.2

Before starting the system ensure that the input pressure of the SampleRail Pneu-matic Unit is set to the values shown in "Compressed Air Requirements" onpage 97, and that all the SampleRail hoods and enclosures are closed.

Start the system by switching on the mains power switch on the rear panel of theSampleRail Control Unit (see Figure 4.5. Control Unit Rear Panel on Page 31).

The SampleRail initialization starts and the trolly moves into its home position (atthe preparation robot). During initialization you should see the following display:

When SampleRail is already on you can trigger the initialization by pushing theRESET button on the SampleRail control unit´s front panel.

EJECT CONT RESETINSERT

Display

SAMPLE RAILINITIALIZATION


Operating Instructions

When the SampleRail system initialization is finished and the trolly has reachedits home position the following is displayed:

SampleRail is now ready to work in automatic mode.

Automatic Mode 6.3

In automatic mode the SampleRail is controlled by the IconNMR and Sample-Track software on the spectrometer host computer. To learn more about thesesoftware tools refer to the individual IconNMR (P/N H9199) and SampleTrackmanuals.

The communication between the spectrometer host computer and the SampleRailcontrol unit (via RS232 connection) is described in "Software Commands" onpage 51.

Manual Insertion Mode 6.4

With SampleRail you have the opportunity to insert or eject samples manuallywithout automation running.

Switching to Manual Insertion Mode 6.4.1

To enter manual mode you must first push the stop button for automation mode inICON-NMR. To switch to manual insertion mode push both the EJECT and theINSERT button and hold for about two seconds until you see the following display:

Switching to manual insertion mode is only possible if the automation is not run-ning.

During automation, the manual insertion mode is inhibited. When the system is inmanual insertion mode it switches back to automation mode as soon as automa-tion is started.

BRUKERSAMPLE RAIL* *

* *

MANUAL INSERTIONMODE


Manual Insertion Mode

Manual Insert 6.4.2

In manual insertion mode, SampleRail will automatically eject an old sample, so ifyou want to exchange samples you don’t have to eject it first.

Ensure there is no sample left in the eject position of the trolly (marked with an’E’). Put the sample to insert into the insert position of the trolly (marked with an’I’). To insert the sample into the NMR magnet push the INSERT button on thefront panel. The trolly will move to the NMR magnet, if a sample is in the magnet itwill be lifted out of the magnet onto the trolly. The new sample will then be put intothe magnet and the trolly will move back to the home position. During the insertionprocess you will see the following display:

If you have not put a sample into the insert position pressing the INSERT button isjust ignored, the trolly will stay in the home position.

If there is a sample left in the eject position of the trolly when you press the IN-SERT button, the insert process is inhibited and an error message ’75 EJECT PO-SITION NOT EMPTY’ is displayed.

In that case press the CONT button to quit the error message, remove the sampleleft in the eject position and restart the insert process by pushing the INSERT but-ton.

Manual Eject 6.4.3

Ensure there is no sample left in the eject position of the trolly. To eject a sampleout of the NMR magnet push the EJECT button on the front panel. The trolly willmove to the NMR magnet, the sample will be lifted out of the magnet onto the trol-ly and the trolly will move back to the home position. During the ejection processyou will see the following display:

When the trolly is back in home position you can take the sample out of the trolly.

INSERTING* ** *

* ERROR ¨ *75 EJECT POSITIO

EJECTING* ** *


Operating Instructions

If there is a sample left in the eject position of the trolly when you press the EJECTbutton, the eject process is inhibited and an error message ’75 EJECT POSITIONNOT EMPTY’ will be displayed.

In this case press the CONT button to quit the error message, remove the sampleleft in the eject position and restart the eject process by pushing the EJECT but-ton. The following figure shows the EJECT and INSERT position (with a sample inthe insert position).

Figure 6.2. Eject and Insert Position in the SampleRail Trolly

The Occurrence of a Failure 6.5

If a failure occurs during operation, the BSR interrupts the current procedure,causing the red „CONT“ LED on the front panel to flash on and off. An error mes-sage, consisting of a code number and text, is also displayed. An error signal isthen transmitted to the spectrometer host computer.

To resume operation after the error has been corrected, press the „CONT“ button.The BSR will then continue at the same point it had reached just before the failureoccurred. The spectrometer host computer will be informed of the successful cor-rection. On rare occasions it may be necessary to do a new initialization, if theabove mentioned intervention did not correct the error.

* ERROR ¨ *75 EJECT POSITIO

INSERT POSITION

EJECT POSITION


7Software Commands 7

SampleRailTM Command Implementation 7.1

Release: BSR20041015_0920

A command always consists of two letters which in some cases are followed by aspace and a parameter. The space between the command and the parameter isessential. A command must always be terminated with <CR>. If this syntax is notfollowed, SampleRail displays the message: "Invalid Parameter" or "Invalid Com-mand".

The syntax of the acknowledge is: [value]<CR><LF>. Value is an alphanumericstring and can have 0 to 32 characters.

SampleRail software commands are divided into two modes:

1. Operation Mode

This is the actual SampleRail operating mode in which complete sampleexchange sequences can be run by a host computer.

2. Diagnostic Mode

This mode offers the user a range of commands to test all the differentsample changer functions.

The use of Diagnostic Mode requires detailed knowledge about the functionof the commands. Only trained service personnel should use DiagnosticMode for tests, diagnostic or adjustment. Incorrect use of Diagnostic Modecommands may cause damage to the equipment.

The different commands are described in detail in the following sections.


Software Commands

Operation Mode 7.2

The following list gives a detailed description of all the operation commands in al-phabetical order.

Standard Commands

Table 7.1. Standard Commands

Instruction: CL (Clear Label)

Format: CL XXX<CR>

Description: Clear the stored label of the sample in trolley insert position

Reply: <CR><LF>

______________________________________________________________

Instruction: CM (Change Mode)

Format: CM<CR>

Description: Reports if Fast Change Mode is On or Off

Reply: CM1<CR><LF> if yes orCM0<CR><LF> if no

______________________________________________________________

Instruction: CT (Clear Time trigger)

Format: CT<CR>

Description: Clear the time trigger for the next sample preparation

Reply: <CR><LF>

______________________________________________________________

Instruction: E1 (Eject sequence part 1)

Format: E1<CR>

Description: Starts an eject sequence

Reply: <CR><LF>

______________________________________________________________

Instruction: E2 (Eject sequence part 2)

Format: E2<CR>

Description: Ends an eject sequence.

The trolley will go to the insert position above the magnet or back to the home position depending on whether a new sam-ple is in the insert position of the trolley or not.

Reply: <CR><LF>


Operation Mode

Instruction: FC (Fast Change Mode)

Format: FC X<CR>

Description: Sets the status of the sample change mode.

X=0 fast change mode is off X=1 fast change mode is on

Reply: <CR><LF>

______________________________________________________________

Instruction: FA (File Available)

Format: FA<CR>

Description: Report if an order file for a preparation is available at the preparation robot

Reply: F1<CR><LF> if yes orF0<CR><LF> if no

______________________________________________________________

Instruction: I1 (Insert sequence part 1)

Format: I1<CR>

Description: Starts an insert sequence

Reply: <CR><LF>

______________________________________________________________

Instruction: I2 (Insert sequence part 2)

Format: I2<CR>

Description: Ends an insert sequence

Reply: <CR><LF>

______________________________________________________________

Instruction: IP (Sample in Insert Position)

Format: IP<CR>

Description: Reports if there is a sample is in the insert position of the trolley when the trolley is in home position or in eject position over the magnet (fast change mode).

Reply: I1<CR><LF> if there is a sample or if the trolley is not in home position.

I0<CR><LF> if there is no sample

______________________________________________________________



Software Commands

Instruction: PD (Probe Down in magnet)

Format: PD<CR>

Description: Reports if a sample is down in the magnet

Reply: P0<CR><LF> if there is no sampleP1<CR><LF> if there is a sample

______________________________________________________________

Instruction: RL (Report Label)

Format: RL XXX<CR>

Description: Reports the label of the sample to be measured.

Reply: L<number><CR><LF> orL0<CR><LF> if there is no label available

______________________________________________________________

Instruction: RP (Report measurement Position)

Format: RP<CR>

Description: reports the current measurement position (command only for BACS compatibility)

Reply: P<number><CR><LF> orP0<CR><LF> if there is no sample in the magnet

______________________________________________________________

Instruction: RT (Report Tube ID)

Format: RT<CR>

Description: Reports the label of the actual measured sample in magnet.

Reply: L<number><CR><LF> orL0<CR><LF> if there is no sample in the magnet

______________________________________________________________

Instruction: SD (move Sample Down)

Format: SD<CR>

Description: If a sample is detected in the magnet after the insert sequence, trolley moves back to home position (only used without fast change mode).

Reply: <CR><LF>

______________________________________________________________



Operation Mode

Instruction: SE (SEcurity test)

Format: ST<CR>

Description: Tests if a sample is left in the magnet.

Reply: S0<CR><LF> if there was no sample in the magnet.S1<CR><LF> if there was a sample in the magnet.

______________________________________________________________

Instruction: SL (Short pulse on Lift)

Format: SL<CR>

Description: Gives a short air pulse on the sample lift to free a hanging sample.

Reply: <CR><LF>

______________________________________________________________

Instruction: ST (Shim sensor Test)

Format: ST<CR>

Description: Tests if a sample is detected in the trolley above shim tube.

Reply: S0<CR><LF> if there is no sampleS1<CR><LF> if there is a sample

______________________________________________________________

Instruction: TD (Keys Disabled)

Format: TD<CR>

Description: locks the front panel keys

Reply: <CR><LF>

______________________________________________________________

Instruction: TE (Keys Enabled)

Format: TE<CR>

Description: unlocks the front panel keys

Reply: <CR><LF>

______________________________________________________________

Instruction: TT (Time Trigger)

Format: TT<CR> or TT XXX<CR>

Description: Trigger for next sample preparation (if a time argument XXX in seconds is sent the next sample preparation will be triggered in XXX seconds) if fast change mode on the trolley moves back to home position.

Reply: <CR><LF>



Software Commands

Instruction: VB (Version Build)

Format: VB<CR>

Description: Reports the actual firmware version inclusive the time of com-pilation and an identification short string.

Reply: BSRyyyymmdd_hhmm<CR><LF>

______________________________________________________________

Instruction: VM (Version Millennium)

Format: VM<CR>

Description: Reports the actual firmware version in Y2K compatible format.

Reply: yyyymmdd<CR><LF>

______________________________________________________________

Instruction: VS (VerSion)

Format: VS<CR>

Description: Reports the actual firmware version.

Reply: yymmdd<CR><LF>

______________________________________________________________

Instruction: ZY (Zero Yell)

Format: ZY<CR>

Description: Repeats the last sent reply

Reply: Last reply<CR><LF>

______________________________________________________________

Instruction: ESC. (ESCape key + ".")

Format: ESC.<CR>

Description: Switch to the Diagnostic mode.

Reply: SAMPLECHANGER DIAGNOSTIC MODE!<CR><LF>



Operation Mode

Table 7.2. Standard Commands for BACS Compatibility

The following commands are only for BACS compatibility, they have no effect on SampleRail:

Instruction: AP(AutoPrep Present)

Format: AP<CR>

Description: Checks if AutoPrep is present.

Reply: BD0<CR><LF>

______________________________________________________________

Instruction: BD (Barcode Digits)

Format: BD XX<CR>

Description: Set barcode length to 4, 6, 12 or 20 digits

Reply: <CR><LF>

______________________________________________________________

Instruction: BS (Report Barcode Setting)

Format: BS<CR>

Description: Report setting of barcode length

Reply: BDXX<CR><LF>

______________________________________________________________

Instruction: CP (Report Position)

Format: CP<CR>

Description: Sends the current magazine position.

Reply: P<number><CR><LF>

______________________________________________________________

Instruction: LS (Sample Lift Status)

Format: LS<CR>

Description: Reports the actual status of the sample lift control

Reply: NL0<CR><LF> if the lift is controlled by SampleRailNL1<CR><LF> if the lift is controlled by BSMS.

______________________________________________________________


Software Commands

Instruction: MS (Number of Measured samples)

Format: MS<CR>

Description: Reports the number of measured samples.

Reply: MS0<CR><LF>

______________________________________________________________

Instruction: NL (No Lift)

Format: NL X<CR>

Description: Sets the status of the sample lift control

X=0 lift is controlled by SampleRailX=1 lift is controlled by BSMS

Reply: <CR><LF>

______________________________________________________________

Instruction: NM (Number of available Magazine positions)

Format: NM<CR>

Description: Reports the number of available magazine positions.

Reply: N192<CR><LF>

N384<CR><LF> (BSR VAC)

______________________________________________________________

Instruction: RC (Restore Control)

Format: RC X<CR>

Description: Sets the restore mode of samples.

Reply: <CR><LF>

______________________________________________________________

Instruction: RS (Report Setting of restore mode)

Format: RS<CR>

Description: Reports the restore mode of samples.

Reply: RCX<CR><LF>

______________________________________________________________

Instruction: SB (Sample in Barcode reader position)

Format: SB<CR>

Description: Reports if a sample is in the barcode reader position.




Error Mode

Error Mode 7.3

In this mode the following two commands will be accepted:

Instruction: SC (SCreen mode)

Format: SC X<CR>

Description: Sets the specific display mode (command only for BACS com-patibility, dummy for SampleRail).

Reply: <CR><LF>

______________________________________________________________

Instruction: SP (Sample present in position)

Format: SP<CR>

Description: For SampleRail test if a sample is present in the insert position of the trolley at the home position.


______________________________________________________________

Instruction: SS (Screen Status)

Format: SS<CR>

Description: Reports the actual display mode.

Reply: SCX<CR><LF>

______________________________________________________________

Table 7.3. Error Mode Commands

Instruction: CO (COntinue)

Format: CO<CR>

Description: Continues the program at the point where it was interrupted when the error showed up.

Reply: <CR><LF>

______________________________________________________________

Instruction: HO (HOme)

Format: HO<CR>

Description: SampleRail moves to its "HOME" position at the preparation robot.

Reply: <CR><LF>



Software Commands

Diagnostic Mode 7.4

In diagnostic mode all operation mode commands are accepted. The message"EXECUTED!" is send before the <CR><LF> when a command is finished.

The additional commands of the diagnostic mode are listed below.

Table 7.4. Diagnostic Mode Commands

Instruction: AB (Adjust Barcode reader)

Format: AB<CR>

Description: Sets the barcode reader into continuous read mode for mechanical adjustment.

Reply: EXECUTED!<CR><LF>

______________________________________________________________

Instruction: AE (Activate Endstop)

Format: AE<CR>

Description: Activates the end stop cylinder above the magnet.


______________________________________________________________

Instruction: CE (Crossing to Eject)

Format: CE<CR>

Description: Moves from the XY-crossing to the eject position above the magnet.


______________________________________________________________

Instruction: CG (Close Gripper)

Format: CG<CR>

Description: Closes the sample gripper above magnet.

Reply: EXECUTED!<CR>

______________________________________________________________

Instruction: CI (Crossing to Insert)

Format: CI<CR>

Description: Moves from the XY-crossing to the insert position above the magnet.


______________________________________________________________


Diagnostic Mode

Instruction: DE (Deactivate Endstop)

Format: DE<CR>

Description: Deactivates the end stop cylinder above the magnet.


______________________________________________________________

Instruction: DS (Display Sensors)

Format: DS<CR>

Description: Displays the actual values of all sensors.

S1: 1 carriage on tecan (S1,X1)2 trolley on tecan (S1,X5)3 sample in insert position (S1,X6)4 sample in insert position (S1,X4)

S2:1 vertical carriage on crossing (S2,X1)2 horizontal carriage on crossing (S2,X2)3 suction tube on position (only BSR VAC con. S2,X6)4 suction tube off position (only BSR VAC con. S2,X4)5 ABW detect (con. S2,X5)

S3:1 endstop cylinder on eject position (S3,X2)2 endstop cylinder on insert position (S3,X3)3 carriage at shim (insert or eject position con. S3,X4)4 trolley at shim (eject position con. S3,X5)5 gripper is open (light barrier sensor con. S3,X6)6 gripper cylinder is active (micro-switch con. S3,X7)7 sample over magnet (fiber-optic sensor con. S3,X11)8 sample down in magnet (sample down unit con. S3,X12)

S4:1 hood at shim (S1,X3) not on BSR VAC2 hood at tecan (S3,X8) not on BSR VAC3 pressure (switch in pneumatic unit)

Reply: S1:xxxx S2:xxxxx S3:xxxxxxx S4:xxx<CR><LF>

______________________________________________________________

Instruction: DT (Display Test)

Format: DT<CR>

Description: Calls the display test program with the character pattern check routine. Pressing the CONT button stops the test.


______________________________________________________________



Software Commands

Instruction: DV (Display Valves)

Format: DV<CR>

Description: Displays the actual values of all valves:

1 bolt cylinder at XY-crossing2 gripper cylinder above magnet3 endstop cylinder above magnet4 horizontal cylinder to XY-crossing5 horizontal cylinder to magnet6 vertical cylinder downwards7 vertical cylinder upwards8 sample lift9 spare valve (BSR VAC position suction tube)

Reply: V1:xxxxxxxxx<CR><LF>

______________________________________________________________

Instruction: EC (Eject to Crossing)

Format: EC<CR>

Description: Moves from the eject position above magnet to the XY crossing


______________________________________________________________

Instruction: EI (Eject to Insert)

Format: EI<CR>

Description: Moves from the eject position above magnet to the insert position above the magnet


______________________________________________________________

Instruction: IC (Insert to Crossing)

Format: IC<CR>

Description: Moves from the insert position above magnet to the XY-crossing


______________________________________________________________

Instruction: IE (Insert to Eject)

Format: IC<CR>

Description: Moves from the insert position above magnet to the eject position above the magnet




Diagnostic Mode

______________________________________________________________

Instruction: IH (Ignore Hood sensors)

Format: IHX<CR>

Description: Moves even with opened doors (for BSR only).

X=1 Ignore hood sensors, X=0 not ignore hood sensors


______________________________________________________________

Instruction: LA (Loop All)

Format: LA<CR>

Description: Starts the continuous test loop program.

First a home seek will be started. You must put two samples into the eject and the insert position of the trolley.

After that the two samples are continuously exchanged and the number of loops will be displayed.

To stop the test loop program press the RESET button.

Reply: 1 Loop(s)<CR><LF>2 Loop(s)<CR><LF>3 Loop(s)<CR><LF>..

______________________________________________________________

Instruction: LD (Lift Down)

Format: LD<CR>

Description: Switches the sample lift off


______________________________________________________________

Instruction: LH (Loop Horizontal)

Format: LH<CR>

Description: This command starts a loop program to test horizontal unit.Stop the endless loop by pressing the RESET button.


______________________________________________________________



Software Commands

Instruction: LL (Lift Loop)

Format: LL<CR>

Description: Starts the continuous lift loop program.

First a home seek will be started. You must put a samples into the eject or the insert position of the trolley. The trolley drives with the position over the magnet, in which the sample is.

After that the sample are continuously inserted and ejected. The number of loops will be displayed.

To stop the test loop program press the RESET button.

Reply: 1 Loop(s)<CR><LF>2 Loop(s)<CR><LF>3 Loop(s)<CR><LF>..

______________________________________________________________

Instruction: LU (Lift Up)

Format: LU<CR>

Description: Switches the sample lift on


______________________________________________________________

Instruction: LV (Loop Vertical)

Format: LV<CR>

Description: This command starts a loop program to test vertical unit.Stop the endless loop by pressing the RESET button.


______________________________________________________________

Instruction: MP (Send MP command to robot)

Format: MP<CR>

Description: Sends a ’MP’ command (Measured Probe) to the preparation robot


______________________________________________________________



Diagnostic Mode

Instruction: NP (Send NP command to robot)

Format: NP<CR>

Description: Sends a ’NP’ command (Next Preparation) to the preparation robot.


______________________________________________________________

Instruction: OA (Send OA command to robot)

Format: OA<CR>

Description: Sends a ’OA’ command (Order file Available) to the prepara-tion robot.


______________________________________________________________

Instruction: OG (Open Gripper)

Format: OG<CR>

Description: Opens the sample gripper above magnet


______________________________________________________________

Instruction: RV (Reset Valve)

Format: RVx<CR>

Description: Resets a specific valve:

x=0 bolt cylinder at XY-crossingx=1 gripper cylinder above magnetx=2 endstop cylinder above magnetx=3 horizontal cylinder to XY-crossingx=4 horizontal cylinder to magnetx=5 vertical cylinder downwardsx=6 vertical cylinder upwardsx=7 sample liftx=8 spare valve (BSR VAC position suction tube)


______________________________________________________________

Instruction: SF(Suction tube in ofF position)

Format: SF<CR>

Description: Moves the suction tube to the OFF position (only BSR VAC).


______________________________________________________________



Software Commands

Instruction: SI (Send SI command to robot)

Format: SI<CR>

Description: Sends a ’SI’ command (Sample ID) to the preparation robot


______________________________________________________________

Instruction: SN (Suction tube in oN position)

Format: SN<CR>

Description: Moves the suction tube to the ON position (only BSR VAC).


______________________________________________________________

Instruction: SV (Set Valve)

Format: SVx<CR>

Description: Sets a specific valve:

x=0 bolt cylinder at XY-crossingx=1 gripper cylinder above magnetx=2 endstop cylinder above magnetx=3 horizontal cylinder to XY-crossingx=4 horizontal cylinder to magnetx=5 vertical cylinder downwardsx=6 vertical cylinder upwardsx=7 sample liftx=8 spare valve (BSR VAC position suction tube)


______________________________________________________________

Instruction: VD(Vertical Down)

Format: VD<CR>

Description: Moves vertically downward


______________________________________________________________

Instruction: VU(Vertical Up)

Format: VD<CR>

Description: Moves vertically upward


______________________________________________________________



Diagnostic Mode

Instruction: TH (Test Hood sensors)

Format: TH<CR>

Description: Tests whether all hoods are closed (for BSR only)

Reply: EXECUTED!<CR><LF> or hood error

______________________________________________________________

Instruction: ESC? (ESCape key + "?")

Format: <ESC>?<CR>

Description: Switches to operation mode.

Reply: SAMPLECHANGER OPERATION MODE! <CR><LF>

______________________________________________________________



Software Commands


8Communication with the Tecan Robot 8

Introduction 8.1

SampleRailTM works together with a TECAN pipetting robot as a preparation sys-tem. The communication between the SampleRail control unit and the TECANcontrolling PC is via an RS232 link and uses the same protocol as the communi-cation between SampleRail and the spectrometer host computer.

A command always consists of two letters and is always terminated with <CR>.The response for any command is terminated with <CR><LF>. Sometimes only<CR><LF> is sent.

Commands 8.2

The following alphabetical list provides a detailed description of all commands.Table 8.1. Commands for TECAN Robot

Sender: TECAN

Receiver: SampleRail controller

Instruction: ES(Eject Sample)

Format: ES<CR>

Description: TECAN wants to take a sample out of the eject position of the SampleRail trolly. SampleRail is locked for motion.

Reply: <CR><LF>

______________________________________________________________


Communication with the Tecan Robot

Sender: TECAN


Instruction: IS (Insert Sample)

Format: IS<CR>

Description: TECAN wants to put a sample into the insert position of the SampleRail trolly. SampleRail is locked for motion.

Reply: <CR><LF>

______________________________________________________________

Sender: SampleRail controller

Receiver: TECAN

Instruction: MP (Measured Probe)

Format: MP<CR>

Description: SampleRail announces that a measured sample is to be removed from the trolly

Reply: <CR><LF>

______________________________________________________________


Receiver: TECAN

Instruction: NP (Next Preparation)

Format: NPCR>

Description: SampleRail triggers the next sample preparation

Reply: <CR><LF>

______________________________________________________________


Commands


Receiver: TECAN

Instruction: OA (Order File Available)

Format: OA<CR>

Description: SampleRail asks if order files for more preparations are avail-able at the TECAN.

Reply: OA0<CR><LF> if no more order files are availableOA1<CR><LF> if one or more order files are available

______________________________________________________________


Receiver: TECAN

Instruction: SI (Sample Identification)

Format: SI<CR>

Description: SampleRail asks for the label of the sample already placed into the trolly

Reply: SIxxxxxxx<CR><LF>

______________________________________________________________

Sender: TECAN


Instruction: TR (Tecan Ready)

Format: TR<CR>

Description: TECAN unlocks SampleRail after ejecting or inserting a sam-ple

Reply: <CR><LF>


Communication with the Tecan Robot


9Error Handling 9

Introduction 9.1

During operation two kinds of error messages may occur:

• Errors requiring user intervention.

• Errors concerning communication between SampleRail and the host comput-er.

These two kinds of failures are treated differently, and are explained in detail inthe following sections.

Error Messages 9.2

An error message consists of two parts:

• A two-digit error code.

• An explicit error message.

A DEL character (decimal 127) will precede any error message transmitted via theSIO channel. In manual insertion mode the error message will not be sent to thehost computer via RS232 link.

Errors Requiring User Intervention 9.2.1

A full list of error numbers and messages with comments are listed in the followingtable. The messages will be displayed and transmitted through the SIO channel.

Table 9.1. Errors requiring user intervention

Error Code Error Message Description Occurrenc

e

02 DOWNWARD MOTIONFAILED

The slider at the vertical unit did not reach the lower position at the preparation robot within a defined time.

D

03 UPWARD MOTION FAILED The slider at the vertical unit did not reach the upper position at the XY-crossing within a defined time.

D


Error Handling

11 SAMPLE DETECT OVER MAGNET FAILED

The sample did not reach the eject position of the trolley during an start eject sequence (E1).

O

12 INITIALIZATION INSUFFICIENT

The initialization could not be accomplished successfully. The error message is indicated only at the LCD display.

D, O, M

13 SAMPLE DETECT IN MAG-NET FAILED

The sample did not reach the position down in the shim system during an end insert sequence (I2).

O

14 SAMPLE GRASPING FAILED The sample was not grasped in the trolley over the shim during an eject sequence or after a failed insert.

D, O, M

15 NO SAMPLE IN INSERT POS. DETECTED

The sample was not detected after an insert from the Tecan robot

O

23 SAMPLE MISSING The sample did not reach a position in the trolley or down in the shim system during an insert or eject sequence.

O, M

26 INSERT POSITION NOT EMPTY

A sample in the insert position was detected. O

53 FAILED TO ACTIVATE SUC-TION TUBE

The suction position was not reached (only BSR VAC).

D, O, M

54 FAILED TO DEACTIVATE SUCTION TUBE

The suction off position was not reached (only BSR VAC).

D, O, M

55 SAMPLE DETECT IN MAG-NET

A sample in the shim system was detected. D, O

56 COMMAND ARE NOT AVAIL-ABLE!

Command in this constellation is not permit-ted.

D, O

60 HORIZONTAL MOTION TO INSERT POS. FAILED

The slider at the horizontal unit did not reach the insert position above the magnet within a defined time.

D, M

61 HORIZONTAL MOTION TO EJECT POS. FAILED

The slider at the horizontal unit did not reach the eject position above the magnet a defined time.

D, M

62 HORIZONTAL MOTION TO CROSSING FAILED

The slider at the horizontal unit did not reach the XY-crossing within a defined time.

D

63 FAILED TO ACTIVATE END-STOP EJECT POS.

The endstop above the magnet did not move to the eject position.

D

65 FAILED TO OPEN GRAB The gripper in the trolley above the magnet did not open.

D, O

66 FAILED TO CLOSE GRAB The gripper in the trolley above the magnet did not close.

D, O



e


Error Messages

Occurrence: D = Diagnostic ModeO = Operation ModeM = Manual Insertion Mode

In the test routines ’Loop All’ and ’Lift Loop’ special error messages are sentwhen errors occur. The error numbers begin with “L”. A description of the error fol-lows the error number.

67 FAILED TO MOVE TO HOME POS.

Search of the home position failed. D, M

68 MOTION TO EJECT POSI-TION FAILED

The trolley did not reach the eject position above the magnet within a defined time.

O

69 MOTION TO INSERT POSI-TION FAILED

The trolley did not reach the insert position above the magnet within a defined time.

O

70 MOTION TO ROBOT STA-TION FAILED

The trolley did not reach the preparation robot within a defined time.

O, M

71 ENDLESS LOOP FAILED The endless test loop stopped when a motion failure or a missing sample occurred.

D

72 HOOD AT ROBOT OPENED The hood at the transfer unit SampleRail / robot is open. For safety purposes all further motion is prevented. When the hood is clo-sed, press ’continue’.

O

73 HOOD AT MAGNET OPENED The hood at the transfer unit SampleRail / magnet is open. For safety purposes all fur-ther motion is prevented. When the hood is closed, press ’continue’.

O

74 SAMPLE RAIL LOCKED BY TECAN

The trolley cannot move because the prepara-tion robot locks the motion while inserting a sample into or ejecting a sample out of the trolley.

O

75 EJECT POSITION NOT EMPTY

In Diagnostic and Operation Mode: a sam-ple is detected in the eject position above the magnet during an eject sequence.

In Manual Insertion mode: a sample is detected in the eject position at the prepara-tion robot when the EJECT or the INSERT button was pressed. The error message is not sent to the host computer via RS232 link.

D, O, M

76 TECAN ROBOT NOT RESPONDING

The TECAN preparation robot did not answer a command within a defined time.

D, O

78 ERROR IN TECAN COMMU-NICATION

The answer string of the TECAN preparation robot was corrupt.

D, O



e


Error Handling

If one of the above mentioned failures is observed, the program branches off intoan error correction routine. It can be restarted by pressing the CONT button or byentering the command „CO’’ via the RS 232C link. As an alternative the command„HO’’ can be used to move the SampleRail into its „HOME’’ position. If a failurecannot be corrected in this manner you will have to restart the whole automationsystem (SampleRail, the preparation robot´s control software, and the spectrome-ter automation software).

If the system still hangs or runs into the same error state after a complete automa-tion restart, consult the operating instructions, or contact a BRUKER service rep-resentative.

Communication Errors 9.2.2

Failures in the communication between the SampleRail and the host computermay occur if an incorrect command is given by the host computer, or if unconnect-ed hardware is addressed. Following such an error message, SampleRail will ac-cept a new command and not enter error mode. In this case, error messages arenot displayed, but are sent back to the host computer. If any of these errors occurconsult your operating instructions, or contact a BRUKER service representative.

Occurrence: D = Diagnostic ModeO = Operation ModeM = Manual Insertion Mode

Table 9.2. Errors in the Communication Between SampleRail and Host Computer


e

51 INVALID COMMAND The received command was invalid. O

52 INVALID PARAMETER The command contained an invalid or incorrect parameter.

O

59 BUSY The command cannot be executed as a concurrent sequence of commands is not yet finished.

O


10Operator Maintenance 10

Introduction 10.1

SampleRailTM requires only a few operator maintenance steps.

Carry out maintenance work only with the SampleRail switched off and the com-pressed air system depressurized. Failure to do this may result in body parts be-ing crushed.

If the horizontal or vertical unit’s pneumatic linear drive leaks, moves slowly, or isjerky maintenance work is necessary. This work can only be done by trained ser-vice personnel according to the operating instructions from the cylinder manufac-turer.

Cleaning 10.2

If you find attrition on the horizontal or vertical unit rails, clean them with a dry, lintfree rag. Do not use cleaning solvents. After cleaning the rails, it is recommendedthat you grease the sliding surface of the pneumatic linear drive (see "Greasingthe Pneumatic Linear Drives" on page 78).

For cleaning the hoods/enclosures you can use any non-aggressive cleaning liq-uid (e.g. dishwasher liquid).


Operator Maintenance

Greasing the Pneumatic Linear Drives 10.3

Figure 10.1. Horizontal Cylinder

The sliding surface of the horizontal and the vertical units pneumatic linear drivesshould be greased annually. Use slow speed grease for pneumatic cylinders (P/N68482) which you will find in the tool chest in the standard accessories deliveredwith your SampleRail.

First clean the sliding surface with a dry, lint-free rag. Then apply a thin film ofgrease onto the surface of the entire length of the pneumatic linear drives.

Figure 10.2. Vertical Cylinder

Sliding surface of the horizontal cylinder

Sliding surface ofthe vertical cylinder


Cleaning After an NMR-Tube Breaks

Cleaning After an NMR-Tube Breaks 10.4

When an NMR tube breaks it should be cleaned up immediately to preventinjury from glass pieces and/or harmful liquids. Cleaning should be donewith the upmost caution, particularly when hazardous liquids are used. Formore information read the safety instructions "Hazards from Samples,Breakage of Glass... and Other Substances" on page 15.

• After a tube breaks, immediately clean the glass parts and liquids from Sam-pleRail (e.g. with small vacuum cleaner) and remove the hose to the magnet.

• Clean the parts with water or a mild cleaning solvent.

• The shim upper part and probe should be inspected, cleaned and repaired byauthorized personnel only.

• SampleRail should only be returned to use if the parts mentioned above are inperfect condition.

Only for the BSR VAC: Removal of glass fragments out of the shim upper part ispossible using the spiral provided in the accessories kit.

Pivoting the SampleRail Horizontal Arm 10.5

For servicing it may be necessary to pull the horizontal arm away from its normalposition. For this reason the vertical and horizontal pivoting arrangement havebeen built with two joint components.

Figure 10.3. Horizontal Arm Joint Components



Since each SampleRail installation is customer specific (ceiling and wall mount-ings and fixtures), the operational procedure for pivoting may differ.

Versions without joint components can only be rotated by moving the TECAN ta-ble.

Be careful not to tilt the construction when working with the fastening.

Before any maintenance: Switch off the electrical power supply on the back of theBSR Control Unit and unplug the power cord.


Pivoting the BSR

Pivoting the BSR 10.6

1. Disconnect the compressed air hose from the lift air valve.

2. Detach the transport tube from the shim upper part.

12

3 3. Disconnect the BSR connector from the Sample Down Unit.

4. Disconnect the fixation screw from the lower joint component.

4



Figure 10.4. Workflow for Rotating the BSR

5. Disconnect the fixation screw from the upper joint component.

6

6. Unscrew the upper screw of the con-necting piece between the horizontal arm and the suspension.

Do not completely unscrew the con-necting piece from the suspension. This will allow simpler reassembly in the same position.

Raise the horizontal arm slowly and push it sideways away from the con-necting piece.

7. The BSR can now be turned. The di-rection of rotation depends on the con-struction of BSR. When rotating the BSR make certain that no cables or hoses are damaged.

8. To return the BSR to its operating po-sition, repeat the steps in reverse or-der. Afterwards, control all the connections to ensure they are tight.

5

7


Pivoting the BSR

Pivoting the BSR VAC 10.6.1

Figure 10.5. Workflow for Rotating the BSR VAC

1. Loosen the lock screw at the lower joint component.

1

2

2. Loosen the lock screw at the upper joint component.

33. Loosen and remove the bolts from the

attachment bracket.



5. Loosen and remove the bolt holding the bracing over the Tecan robot.

6. Loosen and remove the bolt holding the bracing over the Tecan robot.

The BSR VAC can now be rotated. The direction of rotation depends on the construction of the BSR VAC. When rotating the BSR VAC make certain that no cables or hoses are damaged.

To return the BSR VAC to its operating position, repeat the steps in reverse or-der. Afterwards, control all the connec-tions to ensure they are tight.

5

6

4. Take off the lead from the 2D barcode scanner. The fastening for the RJ45 plug can be opened with a small screwdriver.

4


Opening the BSR Cover

Opening the BSR Cover 10.7

The protective hoods / enclosures must not be opened when SampleRail is in op-eration (see "Important Safety Considerations" on page 13).

Removing the BSR Covers 10.7.1

Vertical Hood

The vertical hood is secured using a spring-actuated lock and, beginning in2005, with an additional screw at the front which helps prevent unintention-al opening. To open the hood this screw must be unscrewed.

Transfer Station Protection Hood

The transfer station protection hood over the magnet can be removed bylifting it upwards.

Both hoods contain micro-switches which prevent movement from being initiatedwhen the hood is open (beginning with firmware version: BSR_V20041015 an er-ror message will be displayed). A movement in progress is not interrupted whenthe hood is opened.

Figure 10.6. The Vertical Hood



Opening of the BSR VAC Hood 10.7.2

The protective enclosure for the BSR VAC consists of a vertical cover with aspring-actuated lock, and a horizontal cover which is secured with screws.

The vertical cover must be removed before the horizontal cover can be opened.

Figure 10.7. Removing the Vertical Covering

2. Open the 6 catches at the front of the cover and remove the cover.

2

1. Loosen the screw on the vertical cover which prevents unintentional opening.

1


Opening the BSR Cover

Figure 10.8. Removing the Horizontal Covering

3. The upper cover enclosures can then be opened (unfolded).

1. The horizontal cover is also secured with two screws (left and right) which prevent unintentional open-ing. Loosen these screws. 1

2

33

2. Remove the cover plate.


11Barcode Reader Option 11

Introduction 11.1

For the safe identification of the NMR samples (allocation to preparation order) anoptional barcode reader can be used with SampleRailTM.

Two barcode reader versions are available:

• A stationary CCD barcode reader P/N HZ10192

• A stationary 2D barcode reader P/N H14100

The barcode reader is attached to the SampleRail control unit at the connector la-beled BARCODE. Data transfer is made via an RS232 connection, configuredwith the following parameters:

Baud rate: 9600

Data bits: 8

Parity: no

Stop bit: 1

Protocol: no

When a barcode reader is used with the BSR control unit, it will be automaticallyrecognized when the control unit is turned on or reset. A Sample-ID and Tube-IDare automatically generated.


Barcode Reader Option

Definition of the ’Sample-ID’ and ’Tube-ID’ used within the SampleRail system:

• The Sample-ID is the identification of the sample used during delivery of thesample to the Tecan robot and during the preparation within the robot. TheSample-ID is attached as a barcode to the vial or it results from the placementof the sample in a well plate.

• The Tube-ID is the (temporary) identification of the sample after the prepara-tion - during the transportation of the sample to the Spectrometer using Sam-pleRail and during the NMR measurement. The Tube-ID is attached as abarcode to the NMR tube.

Operation without a Barcode ReaderWhen a barcode reader is not available, the Tecan robot will use the Sample-IDas the Tube-ID, and send this to the BSR control unit after inserting a sample ontothe trolley.

Operation with a Barcode ReaderWhen a barcode reader is used, the actual Tube-ID is read from the barcode onthe NMR tube and sent to the BSR control unit after a sample is inserted onto thetrolley.

In either case the NMR samples can be positively identified during the whole op-erational sequence, allowing simplified sample exchange and recognition.

The CCD Barcode Reader 11.2

The CCD barcode reader is reconfigured with each initialization of the control unit.The following parameters are used:

• Handshake: Free Running

• Read Mode: Single Read

• Time Out: 1 second

• Bad Read: "BR", CR

• Code 128 and Code 2/5 interleaved

• Redundancy: 4 agreeing readings

• Buzzer: Off

• Suffix: CR

The barcode reader is installed using a mounting plate at the vertical transporta-tion cylinder. The barcode reader can be mounted in the left or right position, de-pending on the SampleRail setup.


The 2D Barcode Reader

Figure 11.1. The CCD Barcode Scanner

The 2D Barcode Reader 11.3

The 2D barcode reader offers a higher reading reliability than the CCD reader,particularly in the case of dirty or defective barcodes. Reading errors may result inthe automation being interrupted or the appropriate sample not being measured.

The configuration of the 2D barcode reader is stored in the reader memory. Thebarcode reader is automatically recognized with each initialization of the controlunit. The following parameters are stored in the reader memory:

• Code 128

• Code 2/5 interleaved

• Beeper: on

• Power hold mode: on

The barcode reader is installed using a mounting plate on the frame of Samp-leRail. The mounting plate can be adapted to the individual SampleRail installa-tion.


Barcode Reader Option

Figure 11.2. The 2D Barcode Reader


12Standard Accessories 12

BSR Accessories 12.1

Sample Accessories 12.1.1

The sample accessories are not included with delivery of the BSR and need to beordered separately.

Figure 12.1. SampleRail Sample Accessories

Table 12.1. BSR Sample Accessories

Accessory Part No.

Spinner SB Pla (Pom) 5+2,5 Z42516

Sample Tube 5 mm 7" Royal Imp. H100

Sample Tube Caps 5 mm 100st 8529

Set Barcode Holder Vertical Barcode X100 D=5.1 H10113

H100Z42516H101138529


Standard Accessories

Tool Accessories 12.1.2

The following standard accessories are delivered with the BSR: Table 12.2. Standard Accessories BSR (P/N H10006)

Accessory Part No.

Slow speed grease for pneumatic cylinders without piston rod 68482

Wrench set for socket head cap screws 69806

Screwdriver for cross-slotted screws size 1 9117


Screwdriver 2.5 x 0.5 69880



Adjusting device for transfer unit SampleRail / magnet HZ07505

Adjusting device for mounting flange of flexible tube HZ07808

Tool chest 69670

This manual. Z31571


BSR VAC Accessories

BSR VAC Accessories 12.2

Sample Accessories 12.2.1

The sample accessories are not included in delivery of the BSR VAC and need tobe ordered separately.

Figure 12.2. BSR VAC Tube Assembly Complete

Figure 12.3. BSR VAC Disposal Adapter Spinner

Table 12.3. Sample Accessories for BSR VAC

Accessory Part No.

BSR VAC Disposal Collar 4,25 mm Imp HZ12517

BSR VAC Barcode bracket 500 Pcs H10165

BSR VAC Septum D=10.3/t=1.3 mm Cross Lot HZ12714

MATCH Sample Tube 4.25x100 mm 10 pcs. HZ12654

BSR VAC DISPOSAL ADAPTER 60MM HZ12531

BSR VAC Spinner Retainer New Disposal HZ12442

HZ12654HZ12517HZ12714H10165

HZ12442HZ12531


Standard Accessories

Tools Accessories 12.2.2

The following standard tool accessories are delivered with the BSR VAC: Table 12.4. Standard Tool Accessories BSR VAC (Part No. H12204)

Accessory Part No.

Slow speed grease for pneumatic cylinders without piston rod 68482

Wrench set for socket head cap screws 69806






Adjustment tool transfer BSR VAC / magnet HZ12699

BSR VAC auxiliary tool nozzle- cone HZ13047

BSR VAC auxiliary tool spiral HZ13069

Tool chest 69670

This manual Z31571


13Technical Data 13

Power Supply Requirements 13.1

Input voltage range: 85-264VAC

Input frequency range: 47 - 440Hz

Power consumption: 45VA

Degree of pollution: 2

Over voltage category: 2

Fuse Protection 13.2

2 x 1AT (slow-blow fuse)

Compressed Air Requirements 13.3

Input pressure BSR: 5.0 - 6.0 bar (72 - 87 psi)

Input pressure BSR VAC:6.0 - 7.0 bar (87 - 102 psi)

Absolute max. pressure: 8.0 bar (116 psi)

Air consumption: 100 l/minute

Nitrogen or clean, dry and oil-free compressed air is required.

A compressed air supply, complete with a pressure control valve and a stop valve(meeting EN 983 standards) needs to be provided by the customer.

Serial Communication 13.4

The serial communication ports leading from the SampleRail control unit rear pan-el, to the spectrometer host computer, and to the control PC of the preparation ro-bot (marked ’HOST’ and ’TECAN’ respectively) are configured as follows:

9600 Baud

8 Data bits

No parity

1 stop bit

No protocol


Technical Data

Noise Level 13.5

Emitted noise level: < 60dB (A)

Environmental Requirements 13.6

SampleRail should only be used:

• in a standard laboratory environment;

• up to a height of 2.000 meters;

• at a temperature of 15 to 35°C;

• where the highest relative humidity of 80% for temperatures to 31°C, linear de-creasing to 67% relative humidity to 35°C.

SampleRail should only be stored in an area:

• similar to a laboratory environment;

• with a temperature range of 5 to 40°C;

• where the highest relative humidity of 80% for temperatures to 31°C, linear de-creasing to 50% relative humidity to 40°C.


Figures

1 Introduction 9Figure 1.1. The BRUKER SampleRail and the TECAN Robot ..................9

2 Safety Considerations 13Figure 2.1. Warning Symbol at the Hood/Enclosure of SampleRail ........19Figure 2.2. Alternative Warning Symbol on SampleRail .........................20Figure 2.3. Warning Label at the Rear Panel of the SampleRail Controller

............................................................................................20

3 System Description 21Figure 3.1. The Bruker SampleRail System ...........................................21Figure 3.2. SampleRail Communication Block Diagram .........................23Figure 3.3. Example of a SampleRail System ........................................23Figure 3.4. Example of a BSR VAC System ...........................................24Figure 3.5. The BSR SampleRail Type Label .........................................25Figure 3.6. The BSR Control Unit Type Label ........................................25Figure 3.7. BSR Pneumatic Unit Type Label ..........................................25

4 Installation Instructions 27Figure 4.1. Attaching the Horizontal Arm ...............................................27Figure 4.2. Pneumatic Unit Rear Panel ..................................................28Figure 4.3. BSR Pneumatic Connections ...............................................29Figure 4.4. Pneumatic Connections BSR VAC .......................................30Figure 4.5. Control Unit Rear Panel .......................................................31Figure 4.6. Electric Connections BSR ....................................................32Figure 4.7. Electric Connections BSR VAC ............................................33Figure 4.8. BSR Flexible Tube Components at the Shim Upper Part ......34Figure 4.9. BSR VAC Flexible Tube Components at the Shim Upper Part .

............................................................................................38

5 Settings and Adjustments 41Figure 5.1. Valve to Adjust Lift Air Flow .................................................41Figure 5.2. BSR Pneumatic Unit - Rear Side .........................................42Figure 5.3. BSR Sample Down Unit ECL 00 ..........................................43Figure 5.4. BSR Sample Down Unit ECL 01 ..........................................44Figure 5.5. Adjusting the BSR Sample Down Unit ..................................44Figure 5.6. Adjusting the Sample Down Fiber Amplifier .........................45

6 Operating Instructions 47Figure 6.1. The BSR Control Unit Front Panel .......................................47Figure 6.2. Eject and Insert Position in the SampleRail Trolly ................50


Figures

7 Software Commands 51

8 Communication with the Tecan Robot 69

9 Error Handling 73

10 Operator Maintenance 77Figure 10.1. Horizontal Cylinder .............................................................. 78Figure 10.2. Vertical Cylinder .................................................................. 78Figure 10.3. Horizontal Arm Joint Components ....................................... 79Figure 10.4. Workflow for Rotating the BSR ............................................ 82Figure 10.5. Workflow for Rotating the BSR VAC .................................... 83Figure 10.6. The Vertical Hood ............................................................... 85Figure 10.7. Removing the Vertical Covering .......................................... 86Figure 10.8. Removing the Horizontal Covering ...................................... 87

11 Barcode Reader Option 89Figure 11.1. The CCD Barcode Scanner ................................................. 91Figure 11.2. The 2D Barcode Reader ...................................................... 92

12 Standard Accessories 93Figure 12.1. SampleRail Sample Accessories ......................................... 93Figure 12.2. BSR VAC Tube Assembly Complete .................................... 95Figure 12.3. BSR VAC Disposal Adapter Spinner .................................... 95

13 Technical Data 97


Tables

1 Introduction 9

2 Safety Considerations 13

3 System Description 21

4 Installation Instructions 27

5 Settings and Adjustments 41

6 Operating Instructions 47

7 Software Commands 51Table 7.1. Standard Commands ..................................................... 52Table 7.2. Standard Commands for BACS Compatibility ................. 57Table 7.3. Error Mode Commands .................................................. 59Table 7.4. Diagnostic Mode Commands .......................................... 60

8 Communication with the Tecan Robot 69Table 8.1. Commands for TECAN Robot ........................................ 69

9 Error Handling 73Table 9.1. Errors requiring user intervention ................................... 73Table 9.2. Errors in the Communication Between SampleRail and Host

Computer 76

10 Operator Maintenance 77

11 Barcode Reader Option 89

12 Standard Accessories 93Table 12.1. BSR Sample Accessories .............................................. 93Table 12.2. Standard Accessories BSR (P/N H10006) ...................... 94Table 12.3. Sample Accessories for BSR VAC .................................. 95Table 12.4. Standard Tool Accessories BSR VAC (Part No. H12204) 96

13 Technical Data 97


Tables


Index

A

Accessories......................................................................................................... 93Air pressure

Input .......................................................................................................... 42Applications........................................................................................................... 9Automatic Mode .................................................................................................. 14Automatic mode .................................................................................................. 48

B

Barcode Reader2D ............................................................................................................. 91CCD .......................................................................................................... 90Operating without...................................................................................... 90

Barcode reader ................................................................................................... 89BSR VAC

Description ................................................................................................ 24

C

CCD barcode reader ........................................................................................... 89Cleaning.............................................................................................................. 17Commands.......................................................................................................... 51

Diagnostic Mode Commands.................................................................... 60Error Mode Commands............................................................................. 59For TECAN Robot..................................................................................... 69Standard commands................................................................................. 52Standard commands for BACS compatibility ............................................ 57Structure of a command............................................................................ 69

Compressed air................................................................................................... 28Compressed air supply ....................................................................................... 42CONT.................................................................................................................. 50Control Unit Rear Panel ...................................................................................... 31Customer responsibilities .................................................................................... 18

D

DescriptionSubassemblies.......................................................................................... 21

Diagnostic Mode ................................................................................................. 51

E

Electric ConnectionsBSR........................................................................................................... 32


Index

BSR VAC .................................................................................................. 33Electrical Connections......................................................................................... 31Error Messages................................................................................................... 73

Communication Errors .............................................................................. 76Errors requiring user intervention.............................................................. 73Structure ................................................................................................... 73

Errors .................................................................................................................. 73

F

Failure ................................................................................................................. 50Fiber Amplifier

Adjustment ................................................................................................ 45First Start............................................................................................................. 15Front Panel Controls ................................................................................... 47 – 48

G

Glass tubes ......................................................................................................... 15

H

Hazards............................................................................................................... 15Horizontal Unit

Length ....................................................................................................... 21

I

Initialization ......................................................................................................... 47Installation ..................................................................................................... 18, 27

Flexible tube connection BSR................................................................... 34Flexible tube connection BSR VAC .......................................................... 38

L

LIMS...................................................................................................................... 9

M

Maintenance........................................................................................................ 16Manual Eject ....................................................................................................... 49Manual Insertion Mode........................................................................................ 14Manual insertion mode................................................................................ 48 – 49Manual mode ...................................................................................................... 48

O

Operation ............................................................................................................ 16Operation mode commands................................................................................ 52Operator Maintenance ........................................................................................ 77

After tube breaks....................................................................................... 79Cleaning.................................................................................................... 77


Index

Cleaning solvents...................................................................................... 77Horizontal and vertical units...................................................................... 78Horizontal arm........................................................................................... 79Protective hoods & enclosures ................................................................. 85Sliding surfaces......................................................................................... 78Vertical cylinder......................................................................................... 78

P

Pneumatic Connections ...................................................................................... 28BSR........................................................................................................... 29BSR VAC .................................................................................................. 30

Pressure regulating valve.................................................................................... 42

R

RS232 ................................................................................................................. 69

S

SafetyDangers from moving parts....................................................................... 19Electrical parts .......................................................................................... 20Repair work............................................................................................... 19

Safety instructions............................................................................................... 13Sample accessories ............................................................................................ 95Sample Down Unit .............................................................................................. 44Sample trolley ..................................................................................................... 22SampleRail

Control unit................................................................................................ 22Installation................................................................................................. 27Pneumatic unit .......................................................................................... 22Purpose....................................................................................................... 9Safety instructions..................................................................................... 13Type label ................................................................................................. 25

Settings and Adjustments ................................................................................... 41Air flow for sample lift................................................................................ 41Buffer air for sample lift ............................................................................. 42Sample Down Unit .................................................................................... 43Suction air for BSR VAC sample lift.......................................................... 42Temperate air flow for BSR VAC .............................................................. 43

Starting the system ............................................................................................. 47Structural requriements....................................................................................... 27

T

TECAN.......................................................................................................... 22, 69TECAN Genesis Freedom .................................................................................... 9TECAN pipetting robot ........................................................................................ 21Tools ................................................................................................................... 94Trademarks ......................................................................................................... 10Transfer Unit ....................................................................................................... 22Transportation ..................................................................................................... 14


Index

V

Vertical UnitLength ....................................................................................................... 21

Vibrations ............................................................................................................ 27



Notes:

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