PBS Group | Productive Business Solutions...Created Date 11/13/2019 1:37:37 PM
BSA Solutions in PBS
14
PBS Solutions 1X or 10mM PBS solution total 1 Ltr Take the following in 800mL water Amount in gms Mol Wt No of Moles Molar Concentration / Ltr NaCl 8 gms 58.44 0.14 136.89 KCl 0.2 gm 74.55 0.00 2.68 Na2HPO4 1.44 gms 141.96 0.01 10.14 KH2PO4 0.24 gm 136.09 0.00 1.76 TOTAL 151.48 Check pH and adjust to 7.4 Then add 200mL DI water to make 1 Ltr solution 0,5X or 5mM PBS solution total 1 Ltr Take the following in 800mL water Amount in gms Mol Wt No of Moles Molar Concentration / Ltr NaCl 4 gms 58.44 0.06844627 68.45 KCl 0.1 gm 74.55 0.001341382 1.34 Na2HPO4 0.72 gms 141.96 0.005071851 5.07 KH2PO4 0.12 gm 136.09 0.000881769 0.88 TOTAL 75.74 Check pH and adjust to 7.4 Then add 200mL DI water to make 1 Ltr solution
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Transcript of BSA Solutions in PBS
PBS Solutions
1X or 10mM PBS solution total 1 Ltr
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 8 gms 58.44 0.14 136.89KCl 0.2 gm 74.55 0.00 2.68Na2HPO4 1.44 gms 141.96 0.01 10.14KH2PO4 0.24 gm 136.09 0.00 1.76
TOTAL 151.48
Check pH and adjust to 7.4Then add 200mL DI water to make 1 Ltr solution
0,5X or 5mM PBS solution total 1 Ltr
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 4 gms 58.44 0.06844627 68.45KCl 0.1 gm 74.55 0.001341382 1.34Na2HPO4 0.72 gms 141.96 0.005071851 5.07KH2PO4 0.12 gm 136.09 0.000881769 0.88
TOTAL 75.74
Check pH and adjust to 7.4Then add 200mL DI water to make 1 Ltr solution
PBS Solutions
0,1X or 1mM PBS solution total 1 Ltr
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 0.8 gms 58.44 0.01 13.69KCl 0.02 gm 74.55 0.00 0.27Na2HPO4 0.144 gms 141.96 0.00 1.01KH2PO4 0.024 gm 136.09 0.00 0.18
TOTAL 15.15
Check pH and adjust to 7.4Then add 200mL DI water to make 1 Ltr solution
0,05X or 0,5mM PBS solution total 1 Ltr
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 0.4 gms 58.44 0.006844627 6.84KCl 0.01 gm 74.55 0.000134138 0.13Na2HPO4 0.072 gms 141.96 0.000507185 0.51KH2PO4 0.012 gm 136.09 8.81769E-05 0.09
TOTAL 7.57
Check pH and adjust to 7.4
Then add 200mL DI water to make 1 Ltr solution
1,5X or 15mM PBS solution total 1000mL
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 12 gms 58.44 0.205338809 205.34KCl 0.3 gm 74.55 0.004024145 4.02Na2HPO4 2.16 gms 141.96 0.015215554 15.22KH2PO4 0.36 gm 136.09 0.002645308 2.65
TOTAL 227.22
Check pH and adjust to 7.4Then add 200mL DI water to make 1 Ltr solution
0,15X or 1,5mM PBS solution total 1000mL
Take the following in 800mL waterAmount in gms Mol Wt No of Moles Molar Concentration / Ltr
NaCl 0.12 gms 58.44 0.002053388 2.05KCl 0.03 gm 74.55 0.000402414 0.40Na2HPO4 0.216 gms 141.96 0.001521555 1.52KH2PO4 0.036 gm 136.09 0.000264531 0.26
TOTAL 4.24
Check pH and adjust to 7.4Then add 200mL DI water to make 1 Ltr solution
Bovin Serum Albumin 66463.00 daltons
Stock Solution conc to be made 1 mMAmount of above compound used 0.0665 gms/mLAmount needed to make 4mLsolution in 0,5mM PBS 0.26585 gms in 4mL
Stock Solution conc to be made 1 mMAmount of above compound used 0.0665 gms/mLAmount needed to make 4mLsolution in 1mM PBS 0.26585 gms in 4mL
Stock Solution conc to be made 1 mMAmount of above compound used 0.0665 gms/mLAmount needed to make 4mLsolution in 1,5mM PBS 0.26585 gms in 4mL
BSA solution of 10nM required in each concentration of PBS
Amount of BSA stock solution required to make 10nM BSAAmount used in mL
10nM
0.5 mM PBS 0.005 mL of 1mMsolution
0.5 mM PBS 0.005 mL is required tomake 10nM solutions
0.5 mM PBS 0.005 mL in PBS shown
