BS 200 Fixed Time

7/27/2019 BS 200 Fixed Time

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BS-200 Parameter Setting

(Fixed-time)

WendyApr, 2008


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Main Content

Preface

Basic parameter setting

Reagent setting

Calibration and QC setting


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Preface

Here we set UREA as the example forall Fixed-time items on

Generally, Fixed-time item includes: UREA,Cre,


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Basic parameter for UREA Read the reagent insert first, eg as below.


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Input the item name forTest.

UREA


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No. will be generated automatically by the system, eg 1 for the first one.

UREA

1


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Full Nameof the item, can be blank if the user dont want to input anything.

UREA

1


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Standard No. of the item, can be blank or involved with the bar code.

1

UREA


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Reac. Type of the item, can be referred from the reagent insert as ...

1

UREA


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So we can set it as Fixed-timetype.

Its another saying of Fixed-time.

Basic parameter for UREA


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Reac. Type is set as Fixed-time as the reagent insert indicates.

1

Fixed-time

UREA


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Pri. Wave&Sec. Waveshould be selected as reagent insert indicates

UREA

1

Fixed-time


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So we can set it as 340nm for Pri. Wave; since nosecondary wavelength is mentioned, Sec. Wave could beblank.



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Pri. Waveis selected as 340 nm, Sec. Wave is blank.

UREA

1

Fixed-time

340


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Directionof the absorbance changing during the reaction, refer to ...

1

UREA

Fixed-time

340


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So we can find its

Descending

Moreover, From

Reaction Principle,

we can see NADH(routine indicator

under 340nm) is a

to-be-consumed

reactant, which

will be less and

less during testing,

thus, indicating

the absorbance is

descending in the

measurement.



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Directionis selected as Descending.

1

Descending

UREA

Fixed-time

340


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Reac. Time& Incuba. Time for the reaction, refer to ...

1

Descending

UREA

Fixed-time

340


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Mono reagent items' reaction time begins when Sample is dispensed.

Dual reagent items reaction time begins when R2 is dispensed.

The time between S and R2 dispensing is called Incubation time,only dual reagent measurements have incubation time.

R1 Sample

Preheating time Reaction time

To be set from

the insert Fixed 14

cycles


Incubation

time

0 cycle of

reaction time


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Time is all calculated into cycles on BS-200, and 1 cycleequals to 16 seconds.

Roughly speaking, we change minutes into cycles forReac. Time and Incuba. Time as below.


minute cycle

0.5 2

1 4

2 8

3 12

4 16

5 20


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According to the instruction and the minute-cycle conversion table:

The incubation time is be 1 minute, namely 4 cycles;

The reaction time should be 30seconds and 30+60seconds,

namely 2 and 6 cycles.



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Reac. Timeis set as 2 and 6, Incuba. Time as 4.

1

2 6

Descending

UREA

Fixed-time

340

4


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Unitof the item, should be set according to the reagent insert

1

2 6

Descending

UREA

Fixed-time

340

4


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According to the insert, we can set the Unit as mg/dl ormmol/l for serum testing, and g/24h or mmol/24h forurine samples.



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2 6

4

We can set the Unitas mg/dl.

1

mg/dl

Descending

UREA

Fixed-time

340

1


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2 6

4

Precision of the items results, should be referred to the reagent insert

1

Descending

UREA

Fixed-time

340

mg/dl


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If the unit is set as mg/dl, its normal to report results

without decimals. We can set the Precision as 0.



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2 6

4

Precision of the item is set as 0.

1

0

Descending

UREA

Fixed-time

340

mg/dl


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2 6

4

R1, R2 and Sample Volume of the item, should be referred to

1

Descending

UREA

Fixed-time

340

0

mg/dl


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To process with the sample testing, we need 10ul sample, 1000ulR1 and 250ul R2 (namely, the ratio of S/R1/R2 is 1/100/25);

According to the volume range of BS-200 (sample: 3-45ul, R1:

180-450ul; R2: 30-450ul), and the indicated S/R1/R2 ratio;

We can set Sample volume as 3ul, R1 as 300ul, and R2 as 75ul.



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2 6

4

R1, R2 and Sample Volume are set as 300, 75 and 3.

3

300

Descending

UREA

Fixed-time

340

0

mg/dl

751


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2 6

4

Absorbance ofR1 Blank (mono rgt.) and Mixed Rgt. Blank (dual rgt.),

set according to experience or reagent insert; if no idea, can be blank.

1

3

300

Descending

UREA

Fixed-time

340

0

mg/dl

75


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2 6

4

1

3

300

Linearity Range means the reagents measuring range, refer to

Descending

UREA

Fixed-time

340

0

mg/dl

75


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From the part of Linearity, we can get aware of that, theupper limit of Linearity Range is 300 mg/dl, or 50 mmol/l.


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2 6

4

3

300

We can set the upper limit ofLinearity Range as 300 mg/dl.

300

Descending

UREA

Fixed-time

340

0

mg/dl

75


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2 6

4

1

3

300

Linearity Limit, Substrate Limit and Factorshould be set for the

Kinetics tests, so here we can leave them as blank.

Descending

UREA

Fixed-time

340

0

mg/dl

75

300


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2 6

4

1

3

300

Prozone check is set for the turbidimetric tests, which can also be blank.

Descending

UREA

Fixed-time

340

0

mg/dl

75

300


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The basic parameters for UREA havebeen finished

Next, we should set the reagentinformation as following

Reagent parameter for UREA


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Basic reagent information (Position andBottle Type) has been set up

We should set relevant calibration and QC

information for the item

(Please refer to the video of Calibration & QC setting)

Calibration and QC for UREA
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Thank you!

NOTE:Fixed-time items should be set in the same way as UREA.

BS 200 Fixed Time

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