Two ways to improvediabetes care

NycoCard HbA1c and NycoCard U-Albumin

3 minutes test time

1. Add 5 L whole blood to the test tube with reagent. Mix well. Incubate for 2 minutes.

2. Mix again. Apply 25 L reaction mixture to the Test Device.

3. Add 25 L Washing Solution.

4. Read the result within 5 minutes using the NycoCard READER II.

Test procedure NycoCard HbA1c Determines the quality of metabolic control

The importance of good metabolic control

Several studies have shown the importance of good metabolic control in preventing and slowing the progression of diabetic latecomplications - for persons with Type I (DCCT study1) and Type II(UKPDS2,3,4,5) diabetes. Measurement of glycated haemoglobin hasproven to be an important tool in determining the quality of themetabolic control.

Time-saving for you and your patients

NycoCard HbA1c is rapid and provides an accurate HbA1c resultwithin 3 minutes during the patient consultation making a revisitto alter the treatment regime no longer necessary.

Standardisation

The International Federation of Clinical Chemistry (IFCC) hasestablished a working group on glycohaemoglobin standardisation.This will be based on HbA1c, as it is the easiest component todefine. Methods that measure total haemoglobin, such as affinitymethods, can also be standardised against HbA1c, as their respective values correlate well6.

NycoCard HbA1c is standardised according to the recommendations of the ERL laboratory (European Referencelaboratory) at DCCT-level and is certified in accordance with theERL Check-Up Protocol.

Characteristics

Measuring range: 3-18% HbA1c

Reference range: 4.5-6.3% HbA1c

Sample material: 5 L whole blood

Test result within 3 minutes

Precision: A coefficient of variation (CV) below 5% both within and between run.

No interference of haemoglobin variants or derivatives7,8,9

1. Add 50 L urine to a test tube with Dilution Liquid. Mix well.

2. Apply 50 L diluted urine to the Test Device.

3. Apply 50 L Conjugate to the Test Device.

4. Apply 50 L Washing Solution to the Test Device.

5. Read the test result within 5 minutes using NycoCard

READER II.

Test procedure NycoCard U-Albumin Pin-points patients at risk

Why measure urinary albumin concentrations?

Diabetic nephropathy is a frequent and serious secondary complication of diabetes mellitus, leading to increased morbidityand mortality, and to impaired quality of life in persons affected10,11. Early detection of the disease, at the stage of micro-albuminuria, is important for its outcome and progression. If appropriate measures are taken, microalbuminuria can be haltedor even reversed12.

Microalbuminuria is defined as a persistent elevation of the urinary albumin excretion to 20-200 g/min or 20-200 mg/Lwhen using early morning urine10.

Studies have demonstrated that microalbuminuria independentlypredicts cardiovascular morbidity and all-cause mortality in essential hypertension. Monitoring of microalbuminuria is worth-while in order to monitor the effect of anti-hypertensive treatmenton target-organ damage14.

Low-protein diets, lowering blood pressure and the use of anti-hypertensive therapy have all been reported to have a positiveeffect on decreasing the urinary albumin excretion11,13. The DCCTResearch Group has shown the importance of strict glycaemiccontrol in preventing microalbuminuria1.

NycoCard U-Albumin is simple, quick and convenient withinonly 3 minutes a quantitative test result is obtained using theNycoCard READER II.

Characteristics

Measuring range: 5-200 mg/L albumin

Sample material: 50 L urine

Test principle: Solid phase, sandwich-format, immunometric assay using a gold-antibody conjugate.

Precision: In controlled laboratory testing, a coefficient

of variation (CV) of 5-8% is usually obtained.

Availability1100296 NycoCard U-Albumin, 24 tests1114358 NycoCard U-Albumin Control, 2 x 1 x 1.0 mL1042184 NycoCard HbA1c, 24 tests1113022 NycoCard HbA1c Control, 2 x 0.4 mL1113117 NycoCard READER II kit

References1) New Engl J Med 329:977-986, 1993 (340)

2) Lancet 352:837-853, 1998 (2210)

3) Lancet 854-865, 1998 (2220)

4) BMJ 317:703-713, 1998 (2230)

5) BMJ 317:713-720, 1998 (2240)

6) JIFCC 9(2):62-68, 1996 (760)

7) Clinical Chemistry 28(10):2088-2094, 1982 (1400)

C-reactive protein HbA1c Microalbumin D-Dimer

8) Clinical Chemistry 39(8):1717-1723, 1993 (300)

9) Clinical Chemistry 29(3):543-545, 1983 (1410)

10) Exp Clin Endocr & Diab 107(4):244-251, 1999 (5390)

11) Ann Med 29:439-445, 1997 (2050)

12) Diabetes Care 21(7):1076-1079, 1998 (5060)

13) New Engl J Med 341(15):1127-1133, 1999 (5560)

14) J Hypertension 14 (Suppl 2):S89-S94, 1996 (1630)

Axis-Shield PoC AS

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NycoCard READER II One instument, four tests