BRAVO: Superior Design for Handheld Raman

January 28th, 2015 1

Yan Wang, Ph.D.

Presentation at IFPAC Summit PR 2015

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BRAVO Handheld Raman SpectrometerHighlights at a Glance

January 28th, 2015

LASER class 1M product

SSETM – Sequentially Shifted Excitation (Patented)

Duo LASERTM excitation

IntelliTipTM - automated measuring tip recognition

Intuitive and guided touchscreen operation

Automated batch scan reporting

Inbuilt wavenumber calibration

Robust and precise optics

Wireless data exchange

Complies with 21 CFR Part 11 requirements

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BRAVO Handheld Raman SpectrometerHardware

January 28th, 2015

Barcode buttonactivates 2D barcode reader

Laser release button

On/off button BRAVO

Battery door

Keypad

Laser on/off button

Measuring tip (bag tip shown)

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Sequentially shifted excitation – SSETM

Mitigate Fluorescence

January 28th, 2015

J.B. Cooper, M. Abdelkader, K.L. Wise, Appl. Spectros. 67, 973–984 (2013).J.B. Cooper, S. Marshall, R. Jones, M. Abdelkader, K.L. Wise, Appl. Optics. 53, 3333–3340 (2014).

SSETM (patented) method generates Raman spectra in true spectral space does not require generation (and subsequent reconstruction) of derivative spectra (SERDS) results in improved signal-to-noise ratio compared to traditional fluorescence rejection methods while

providing instrument-limited bandwidth resolution

50010001500200025003000Raman Shift / cm-1

Ram

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nsi

ty

-Lactose monohydrate

No impact of Fluorescence

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Sequentially shifted excitation – SSETM

Mitigate Fluorescence

January 28th, 2015

40060080010001200140016001800Raman shift cm-1

Ram

an In

ten

sity

Talc in PE

BRAVOconvenient dispersive Raman spectrometer

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Duo LASERTM Access the CH stretching region

January 28th, 2015

50010001500200025003000Raman Shift / cm-1

Ram

an I

nte

nsi

ty

4-Acetamidophenol

FT-Raman (spectral resolution 8cm-1)

BRAVO

Access to the CH stretching range avoids erroneous verification/identification

Examples:

• Discrimination between different polymorphs of a drug

• Contamination of sample with e.g. cyanide (cannot be observed if the Raman shifts ends at 1800cm -1)

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IntelliTipTM

Always use the proper measuring tip

January 28th, 2015

Inner Spacer

Bag Cap

IntelliTipTM

The locking mechanism design guarantees

quick and highly reproducible connection/

disconnection of measuring tips.

ID chips prevent to release the Laser unless a

tip is connected.

Even more, if a measuring tip is defined in the

library for a chemical material, BRAVO will

inform the user which measurement tip needs

to be used for the verification.

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Intuitive, gesture-based touch screen GUISpeed up your workflow

January 28th, 2015

Validity of Performance Qualification (PQ) and Operational Qualification (OQ)

Drop down menu lists messages, warnings, etc. indicated by the envelope icon on the upper left

Network connectivity

Battery lifetime

General Information

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Intuitive, gesture-based touch screen GUILogin Screen

January 28th, 2015

Enter Username:

hit any letter on the onscreen keyboard (OSK) that is contained in the name you like to enter

Example: hit A and get a list of all names containing A

select the proper entry, or scroll down to find the proper entry, or hit letter next to A to shorten the selection list

Enter Password:

Enter password on OSK

The approach described above will not work!

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Intuitive, gesture-based touch screen GUIMain Screen

January 28th, 2015

Sample Verification will guide through the verification procedure and ends up with an

Accepted / Rejected information

Acquire Materials can be used to acquire spectra for building a library

Review Results allows to review formerly measured spectra still being stored on the

BRAVO

Library Management allows to select/deselect library for verification

Settings allows for basic settings like date, time, LCD brightness, etc. (availability depends on user rights)

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Intuitive, gesture-based touch screen GUISample Verification Workflow

January 28th, 2015

Material Name:Describes the name of a chemical compound to be analyzed in its packaging (example: Talc in PE bag, Talc in vial), this is a required entry

Batch/Lot# and Container# can be filled in but are not mandatory for proceeding

All information can be entered via the OSK in the above explained manner

or by using the 2D barcode reader which can either be activated on the touchscreen by touching the following icon

or by pressing the barcode button on the BRAVO keypad

Start sample verification workflow by touching sample verification icon

Operator

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Intuitive, gesture-based touch screen GUISample Verification Workflow

January 28th, 2015

Once the sample information are completed

Touch the Laser icon

or swipe with the finger in direction of the arrows

Operator

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Intuitive, gesture-based touch screen GUISample Verification Workflow

January 28th, 2015

IntelliTipTM now tells which measuring tip needs to be used

To start the verification:

touch the Press to Start icon

or press Laser release button on the BRAVO keypad

Place Vial into Sample Holder

Operator

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Intuitive, gesture-based touch screen GUISample Verification Workflow

January 28th, 2015

Results

it’s clearly visible whether the verification was Accepted or Rejected

derived spectrum and library spectrum can be viewed on Results screen

touching the barcode icon brings back to the first screen

in case of entries on the first screen differ by Container # only, a message will appear stating how many containers had been measured already

Operator

Batch Scan Touching this icon brings back to the first

screen. Material Name and Batch/Lot # are already filled in.

Results screen

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