BOTANICAL SURVEY OF INDIABOTANICAL SURVEY OF INDIA

ANNUAL SCIENTIFIC MEET 2016-17

DR. TAPAN SEAL

1990-1998 : Technical Assistant

1998-2002 : Plant Chemist

2002-2005 : Examiner of Patents and Designs in the Patent Office

2005-2009 : Plant Chemist

2009-2014 : Scientist B

2014-till date : Scientist C

JOINED THIS ORGANIZATION IN NOVEMBER 1990

BRIEF RESUME OF WORK

AND

SIGNIFICANT ACHIEVEMENT UNDER

ANNUAL ACTION PLAN

• Phyto-chemical Screening of some Leguminosae plants for

active phytoconstituents

• Toxicity studies (LC50) of 30 leguminosae plants using Brine

Shrimp bioassay method

• Antimicrobial activities 10 leguminosae plants

• Chemical and Pharmacological investigation of Kopsia fruticosa

YEAR : 1990-1994

Kopsine, a novel Indole alkaloid was isolated

from the leaves of Kopsia fruticosa and the

relative configurations at various centres has

been achieved by X-ray crystallographic

studies.

• The alkaloid showed CNS depressant action

• The alkaloid potentiates the hypnotic dose of hexobarbitone

• The alkaloid potentiates the cataleptic doses of haloperidol

• The alkaloid was found to have sedative ataxic effect

Acta Cryst., C49, 171-173, 1993

C

O

CH3

N

N

OH

OH

O

YEAR : 1990-1994 contd.

Phytochemical investigation of Tiliacora racemosa

1. A voluminous and comprehensive review on the “Pharmacological

activities of Bisbenzylisoquinoline Alkaloids” was prepared

YEAR : 1995-2000

Journal of Medicinal and Aromatic Plant Sciences, 19, 32-92, 1997

N

O

ON

2. Ten BISBENZYLISOQUINOLINE alkaloids were isolated and identified by sophisticated spectral techniques

1.Tiliacorine2.Tiliacorinine3.Tiliamosine4.Tiliaresine5.Nor tiliacorinine A6.Tiliarine7.N-methyl tiliarine8.N-methyl tiliamosine9.Nor tiliacorinine B10. Tiliacosine

3. Multidimensional structure of Tiliacorine

was established by 2D NMR spectrum

Organic Letters, 2 (24), 3813-15, 2000

YEAR : 1995-2000 contd.

4. Pharmacological investigations with tiliacorine isolated from Tiliacora racemosa was carried out

The crude alkaloid and tiliacorinine showed following activities

• CNS depressant activity

• Neuromuscular blocking activity

• Spasmolytic activity

• Hypotensive activity

• Anticonvulsive activity

• Analgesic activity

Natural Product Sciences, 5(3), 142-147, 1999

Natural Product Sciences, 6 (1), 44-48, 2000

Natural Product Sciences, 6(3), 126-130, 2000

5. in vitro anticancer activity of Tiliarine was established against

human Melanoma cell (G 361) and normal cell (CRL 1656)

Phytotherapy Research, 16, 596-99, 2002

Phytochemical investigation on Mangrove plants

Hypoglycemic activities of a Mangrove plant Rhizophora apiculata Blume.was studied

Phytochemical investigation of Stephania hernandifolia

Cycleanine, a bisbenzylisoquinoline alkaloid was isolated andidentified

Pharmacological investigation of cycleanine showed the followingactivities

• Antiinflammatory activity• Analgesic activity• Anticonvulsive activity

YEAR : 2001 - 2007

Oriental Pharmacy and Experimental Medicine, 3(3),123-128, 2003

Natural Product Sciences, 10(1), 11-15, 2004

Cytotoxicity study (PC12 Cell line) and antioxidant properties of three

medicinal plant viz. Sida cordifolia, Cynodon dactylon and Evolvulus

alsinoides were investigated

Antidiabetic activity of Caesalpinia bonducella F. in chronic type 2 diabetic

model in Long-Evans rats and evaluation of insulin secretagogue property of

its fractions on isolated islets was studied

Phytochemical investigations of Endangered Plant species in India

including Negative List of Export and their Biological assessment

YEAR : 2001 – 2007 contd.

Journal of Ethnopharmacology, 84, 131-138, 2003

Journal of Ethnopharmacology. 10;97(1),117-22, 2005

Chemical Composition and Nutritive Value of Wild

Edible Plants of North-East Region in India

YEAR 2008 - 2016

Literature survey

Estimation of Proximate compound

Collection and identification of plant material

Vitamin estimation

Antioxidant activity

Ash

Estimation of Minerals

Calcium

PLAN OF WORK : BIRDS’ EYE VIEW

Crude fat

Moisture

Protein

Crude fibre

Carbohydrate

Sodium Potassium CopperZincIronManganese Chromium

RESULTS : AT A GLANCE

Plant materials

112 nos. wild edible plants were collected from the different tribal places of

Meghalaya and Arunachal Pradesh

YEAR 2008 – 2016 c o n t d .

YEAR 2008 – 2016 c o n t d .

YEAR 2008 – 2016 c o n t d .

YEAR 2008 – 2016 c o n t d .

ESTIMATION OF VITAMIN BY HPLC

VITAMIN C(Ascorbic acid)

VITAMIN B1 (THIAMINE)

VITAMIN B2(RIBOFLAVIN)

VITAMIN B3(NIACIN)

VITAMIN B5

(Pantothenic acid)VITAMIN B6

(PYRIDOXINE)VITAMINE B9

(Folic acid)

YEAR 2008 – 2016 contd.

5.0 10.0 15.0 20.0 25.0 30.00

250

500

750

1,000

1,200 ALL STANDARD VITAMINmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Ascorbic acid - 7.903

Niacin B3 - 10.227

Pyridoxine B6 - 16.557

Pantothenic acid B5 - 20.503

Folic acid B9 - 23.173

Thiamine B1 - 24.273

Riboflavin B2 - 25.880

WVL:210 nm

5.0 10.0 15.0 20.0 25.0 30.0-5

50

100

150

200 Z. acanthopodium LeavesmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Asc

orbi

c ac

id -

8.6

97

Pyr

idox

ine

B6

- 16

.397

Pan

toth

enic

aci

d B

5 -

20.4

57

Fol

ic a

cid

B9

- 23

.057

Thi

amin

e B

1 -

24.1

83

Rib

ofla

vin

B2

- 25

.840

WVL:245 nm

5.0 10.0 15.0 20.0 25.0 30.0-5

50

100

150

200 V. foetidum fruitsmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Asc

orbi

c ac

id -

7.9

20

Pyr

idox

ine

B6

- 16

.240

Pan

toth

enic

aci

d B

5 -

20.1

50

Fol

ic a

cid

B9

- 23

.373

Thi

amin

e B

1 -

24.2

07

Rib

ofla

vin

B2

- 25

.857

WVL:245 nm

5.0 10.0 15.0 20.0 25.0 30.05

50

100

150

200 S kurzii fruitsmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Asc

orbi

c ac

id -

8.6

67

Nia

cin

B3

- 10

.247

Pyr

idox

ine

B6

- 16

.743

Pan

toth

enic

aci

d B

5 -

20.1

90

Fol

ic a

cid

B9

- 22

.957

Thi

amin

e B

1 -

24.1

20

WVL:245 nm

Vitamin in Z.. acanthopodiumVITAMIN STD

Vitamin in V. foetidum Vitamin in S. kurzii

YEAR 2008 – 2016 contd.

ANTIOXIDANT ACTIVITIES OF WILD EDIBLE PLANTS

• Extractive value with different solvents

• Total phenolic content

• Total flavonoid content

• Total flavonol content

• Reducing power

• DPPH radical scavenging activity

• ABTS radical scavenging activity

YEAR 2008 – 2016 c o n t d .

YEAR 2008 – 2016 c o n t d.

YEAR 2008 – 2016 c o n t d.

PHENOLIC ACIDS

Gallic acid Chlorogenic acid p-Hydroxy benzoic acid

Caffeic acid Syringic acid p-Coumaric acid Sinapic acid

Ferulic acid

Protocatechuic acid

Vanilic acid Gentisic acidEllagic acid

YEAR 2008 – 2016 c o n t d.

2.0 20.0 30.0 40.0 50.0 60.0 70.0 81.0-5

100

200

300 ALL STD PHE 60mAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Gal

lic

acid

- 6

.897

Pro

toca

tech

uic

acid

- 1

4.67

0

Gen

tisi

c ac

id -

26.

290

p-O

H b

enzo

ic a

cid

- 30

.637

Chl

orog

enic

aci

d -

42.0

30 V

anil

ic a

cid

- 43

.623

Caf

feic

aci

d -

45.5

13

Syr

ingi

c ac

id -

49.

177

p-C

oum

aric

aci

d - 56

.117

Fer

ulic

aci

d -

59.3

13 S

inap

ic a

cid

- 59

.990

Sal

icyl

ic a

cid

- 67

.900

Ell

agic

aci

d -

73.7

07

WVL:280 nm

PHENOLIC ACIDS STD

2.0 20.0 30.0 40.0 50.0 60.0 70.0 81.0-5

100

200

300 V. foetidum EtOH extmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Pro

toca

tech

uic

acid

- 1

4.41

0

Gen

tisi

c ac

id -

26.

683

Syr

ingi

c ac

id -

49.

090

p-C

oum

aric

aci

d -

56.0

07

Sin

apic

aci

d -

60.3

93

Sal

icyl

ic a

cid

- 69

.613

Ell

agic

aci

d -

74.2

33

WVL:280 nm

Phenolic acids in V. foetidum

2.0 20.0 30.0 40.0 50.0 60.0 70.0 81.0-5

100

200

300 S. kurjii ETOH extmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Van

ilic

aci

d - 44

.383

Syr

ingi

c ac

id -

49.

960

p-C

oum

aric

aci

d -

56.7

70

Fer

ulic

aci

d - 59

.847

Sin

apic

aci

d -

60.6

90

WVL:280 nm

Phenolic acids in S. kurzii

2.0 20.0 30.0 40.0 50.0 60.0 70.0 81.0-5

100

200

300 S. gilo MeOH extmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Chl

orog

enic

aci

d -

42.9

27

Caf

feic

aci

d -

46.7

33

Syr

ingi

c ac

id -

50.

130

p-C

oum

aric

aci

d -

56.6

53

Fer

ulic

aci

d -

59.3

33 S

inap

ic a

cid

- 60

.537

Sal

icyl

ic a

cid

- 69

.953

Ell

agic

aci

d -

73.1

03

WVL:280 nm

Phenolic acids in S. gilo

YEAR 2008 – 2016 c o n t d.

Flavanoids, Flavonols and Coumarin compounds

Aesculin Catechin Rutin

Naringin Myricetin Coumarin

Quercetin

Apigenin

Kaempferol Naringenin Luteolin

YEAR 2008 – 2016 c o n t d.

5.0 20.0 30.0 40.0 50.0 66.0-1

50

100

150 ALL 11 STD Flavonoid 40mAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Aes

culi

n -

7.52

0

Cat

echi

n -

12.0

77

Rut

in -

22.

947 N

arin

gin

- 31

.497

Myr

ecet

in -

35.

850

Cou

mar

in -

40.

383

Lut

eoli

n -

47.3

87 Q

uerc

etin

- 4

8.32

7

Nar

inge

nin

- 56

.820

Api

geni

n -

57.7

03

Kae

mpf

erol

- 6

0.48

0

WVL:280 nm

FLAVONOIDS STD

5.0 20.0 30.0 40.0 50.0 66.0-5

50

100

150 V foetidum EtOH ExtmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Aes

culi

n -

7.19

0

Cat

echi

n -

10.9

37

Rut

in -

22.

510

Nar

ingi

n -

31.0

93

Cou

mar

in -

40.

123

Nar

inge

nin

- 57

.207

WVL:272 nm

Flavonoids in V. foetidum

5.0 20.0 30.0 40.0 50.0 66.0-5

50

100

150 S.gilo ETOH ExtmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Cat

echi

n - 12

.297

Rut

in -

23.

040

Nar

inge

nin

- 56

.900

WVL:280 nm

Flavonoids in S. gilo

5.0 20.0 30.0 40.0 50.0 66.0-5

50

100

150 P.ocimoides ETOH ExtmAU

min

Abs

orba

nce

[mA

U]

Retention Time [min]

Aes

culi

n -

7.22

0

Cat

echi

n -

14.0

57

Myr

ecet

in -

36.

210

Lut

eoli

n -

46.9

03

Api

geni

n -

57.4

23

WVL:280 nm

Flavonoids in P. ocimoides

YEAR 2008 – 2016 c o n t d.

Chemical, pharmacognostical and glactogogue activity of Asparagus racemosus

Wild. and comparison with different market drugs of Shatavari

Science & Culture , 73 (9-10),309-314, 2007

Antioxidant and antiinflammatory activities of different solvent extracts and

isolated compounds of Ipomoea pes-caprae (L) Sweet of Sunderban Mangrove Eco-

complexAsian Journal ofChemistry;25(9),4997-5000, 2013

Evaluation of galactagogue and antioxidant activities of the root extract ofEuphorbia fusiformis

Asian Journal of Traditional Medicines,8(5), 119-28, 2013

HPLC studies of Cynodon dactylon and its evaluation for wound healing activity

Journal of Ethnopharmacology xxx (2016) xxxxx

COLLABORATIVE WORK

Evaluation of antioxidant activities of TEN algae and effect of solvent extraction system(Nilu Halder , Kalyani University)

International Journal of Pharmacy and Pharmaceutical Sciences, 6 (10), 242-245, 2014International Journal of Pharmaceutical Sciences and Research. 6(3), 1273-78, 2015

Antioxidant of different parts of Lysimachia laxa and Gymnocladus assamicus, acomparison using three different extraction systems(Sanjoy Gupta, ERC, BSI)

Journal of Chemical and Pharmaceutical Research, 5(4) : 33-40 (2013)

Comparative HPLC Fingerprinting and Antioxidant Activities of some in vitro and invivo Grown Orchids(Gargi Prasad SRF, ERC)

Journal of Chemical, Biological and Physical Sciences 6(2), 454-468 (2016)

Nutraceutical evaluation of wild edible plants(Sagari , SRF, CBL)

in vitro antioxidant and antidiabetic activity of Tinospora sinensis

(Anindita Banerjee, Calcutta University)

IJCPR 2017, JABB 2017

Chemical and Biological evaluation of selected species of George Watt listed plants

(Sudeshna Dutta, ISIM)

SUPERVISION OF PH D SCHOLARS

PUBLICATION SINCE 1990Total publication : 56

Major publication in :

Organic letters

Journal of Ethnopharmacology

Natural Product Sciences

International Journal of Pharmacy and Pharmaceutical Sciences

F UTURE P LAN

• Amino acid composition of wild edible plants

• Anti-nutritional properties of wild edible plants

• Toxicity studies of wild edible plants

• in vitro antidiabetic activities of traditionally reported

wild edible plants