Book of Abstracts · The Editorial Board is not responsible for the content of any research paper...

Book of Abstracts

The 3rd International Conference on Bioscience

and Biotechnology

(BioTech 2018)

08th-09th March 2018

Colombo, Sri Lanka

Committee of the BioTech 2018

The International Institute of Knowledge Management (TIIKM)

Tel: +94(0) 11 3132827

[email protected]

ii

Disclaimer

The responsibility for opinions expressed, in articles, studies and other contributions in this

publication rests solely with their authors, and this publication does not constitute an

endorsement by the BioTech or TIIKM of the opinions so expressed in them.

Official website of the conference

www.bioscienceconference.com

Book of Abstracts of the 3rd International Conference on Bioscience and Biotechnology

(BioTech 2018)

Edited by Prof. Mark Smales and Dr. Chamari Hettiarachchi

ISBN 978-955-4903-95-1

Copyright @ 2018 TIIKM Publishing LLC

All rights are reserved according to the code of intellectual property act of Sri Lanka,

2003

Published by The International Institute of Knowledge Management (TIIKM), No:

531/18, Kotte Road, Pitakotte ,10100, Sri Lanka

Tel: +94(0) 11 3098521

Fax: +94(0) 11 2873371

iii

Hosting Partner:

University of Kent, United Kingdom

Academic Partners:

British College of Applied Studies (BCAS) Campus, Sri Lanka

Faculty of Food Technology and Biotechnology, University of Zagreb, Republic of Croatia

Anna University, Chennai, India

University of Malaya, Malaysia

Organized By:

The International Institute of Knowledge Management (TIIKM), Sri Lanka

PROF. MARK SMALES (Conference Co- Chair, BioTech 2018)

Professor of Industrial Biotechnology, Director of the

Industrial Biotechnology, University of Kent, United

Kingdom

DR. CHAMARI HETTIARACHCHI (Conference Co-Chair, BioTech 2018)

Senior lecturer in Molecular Biology and Biochemistry,

Department of Chemistry, University of Colombo

PROF. ATTA-UR-RAHMAN (Keynote Speaker, BioTech 2018)

Professor Emeritus/Patron-in-Chief, International Center

for Chemical and Biological Sciences, (H. E. J. Research

Institute of Chemistry and Dr. Panjwani Center for

Molecular Medicine and Drug Research) University of

Karachi, Karachi, Pakistan

PROF. DR. VLADIMIR MRŠA (Keynote Speaker, BioTech 2018)

Professor of Industrial Biotechnology, University of

Zagreb, Croatia

DR. ANURADHA DHANASEKARAN (Plenary Speaker, BioTech 2018)

Head of the Department of Biotechnology, Anna

University, India

DR. D. N.VINOD KUMAR (Session Chair, BioTech 2018)

National Institute of Oceanography, India

BioTech 2018 Committee

iv

DR. SELVAKUMAR DHARMARAJ (Session Chair, BioTech 2018)

Academy of Higher Education, India

DR. ANBU ARUNKUMAR (Session Chair, BioTech 2018)

Karpagam Academy of Higher Education, India

DR. PAVAN JUTUR (Session Chair, BioTech 2018)

International Centre for Genetic Engineering and

Biotechnology, India

DR. KASHIF AHMED (Session Chair, BioTech 2018)

NED University of Engineering & Technology, Pakistan

MR. ISANKA. P. GAMAGE (Conference Convener, BioTech 2018)

The International Institute of Knowledge

Management, Sri Lanka

MR. OSHADEE WITHANAWASAM (Conference Publication Chair, BioTech 2018)



MS. ASHA RATHNAYAKE (Conference Secretariat, BioTech 2018)



Editorial Board-ICOM 2013

Editors in Chief

Prof. Mark Smales, Professor of Industrial Biotechnology, Director of the Industrial Biotechnology, University

of Kent, United Kingdom

Dr. Chamari Hettiarachchi, Senior Lecturer in Molecular Biology and Biochemistry, Department of Chemistry,

University of Colombo

The Editorial Board is not responsible for the content of any research paper

Editorial Board - BioTech 2018

v

Prof. Oyaziwo Aluede, Department Of Educational Foundations And Management, Ambrose Alli

Dr. Amy Then Yee Hui, University of Malaya, Malaysia

Dr. Anupama Halmillawewa, University of Kelaniya, Sri Lanka

Prof. D. N. Rao, IIMS, India

Dr. Anushka Elvitigala, University of Sri Jayewardenepura, Sri Lanka

Dr. Farshid Talat, West Azarbaijan Agricultural and Natural Resources Research and Education Center, Iran

Dr. M. M. Kabir, East West University, Bangladesh

Prof. Deepal Mathew, University of Colombo, Sri Lanka

Asst. Prof. Azam Noori, Merrimack College, United States

Asst. Prof. Kashmir Singh, Panjab University, India

Dr. Sharmila Jayasena, University of Colombo, Sri Lanka

Dr. Murni Halim, Universiti Putra Malaysia, Malaysia

Dr. Siti Aqlima Ahmad, Universiti Putra Malaysia, Malaysia

Dr. Ahmad Muhaimin Roslan, Universiti Putra Malaysia, Malaysia

Dr. Mohamad Zulfazli Mohd Sobri, Universiti Putra Malaysia, Malaysia

Dr.C.D. Anuradha, Anna University, India

Dr. Mohamad Zulfazli Mohd Sobri, Universiti Putra Malaysia, Malaysia

Dr. Acga Cheng, University of Malaya, Malaysia

Scientific Committee - BioTech 2018

vi

MESSAGE FROM THE HOSTING PARTNER BioTech 2018

Dear Participants,

The University of Kent is delighted to welcome you to the 3rd International Conference on

Bioscience and Biotechnology 2018, Colombo, Sri Lanka, as a hosting partner of the event.

This meeting offers an exciting opportunity for the University of Kent to help foster and

develop new interactions between research scientists to share knowledge and instigate new

collaborations. The development of biotechnology, such that precision genome editing can be

undertaken and large scale sequencing is becoming a ‘routine’ tool of the trade, has given

scientists an unparalleled ability to manipulate and harness the power of biosciences and

apply this to health, agriculture and environmental issues (to name but a few) whilst ensuring

such tools are used in a responsible manner. The meeting here brings together delegates from

a wide range of disciplines to discuss their latest findings and interpretations across the

bioscience and biotechnology remit, providing a forum for the exchange of knowledge and

forging of new ideas.

We welcome you again to the conference and wish you a successful and productive meeting

in Colombo.

vii

MESSAGE FROM THE CONFERENCE CO-CHAIR BioTech 2018

Dear Participants,

It is with great pleasure that the I welcome you on behalf of the organizing committee to the

the 3rd International Conference on Bioscience and Biotechnology 2018 held in the beautiful

city of Colombo, Sri Lanka. This now established conference consists of 2 conference tracks,

Application of Biochemistry and Molecular Biology to Biotechnology and General

Biological Engineering. Within these two tracks there is a range of sessions and topics from

biodrug discovery and manufacturing to bioremediation and from biological systems and

models to nano-medicine. The full details of the wide and cross-disciplinary range of topics is

detailed on the website and abstract handbook. The breath of topics covered reflects the fact

that bioscience and biotechnology plays an important role in many facets of everyday life and

offers the potential to solve local and global challenges that face us all.

The scientific sessions cover cutting edge new science in the areas outlined. The extensive

program has been designed by the committee to give as many early career scientists the

opportunity to presentation alongside the high profile and keynote speakers that we have

attracted to the meeting. We thank all the speakers for agreeing to participate and those who

submitted abstracts. The poster session also provides opportunities for authors to present their

work and we encourage everyone to visit the posters and discuss the work with the authors.

The organizing committee would especially like to thank our sponsors. Their generosity helps

support this conference. I would also like to thank the rest of the organizing committee for

undertaking all their assigned tasks in a timely manner and working together so well.

Finally, the location, the scientific program, and the social events have been designed to

encourage networking and enhance scientific discussion amongst participants. It is

anticipated that the oral and poster presentations in this inspiring environment will generate

new ideas and the initiation of fruitful novel research projects and collaborations. Participants

and their engagement make a scientific meeting and it is up to us all to ensure this is a

successful conference that we all reflect upon favorably. We thank you for participating in

the conference and wish you a successful and productive meeting in Colombo.

Mark Smales

Conference Co-Chair BioTech 2018

Professor of Industrial Biotechnology,

Director of the Industrial Biotechnology,

University of Kent,

United Kingdom.

viii

MESSAGE FROM THE CONFERENCE CO-CHAIR BioTech 2018

On behalf of the organizing committee, it is my honor and pleasure to welcome all research

scientists, academics, postgraduate and graduate students from all over the world to

Colombo, Sri Lanka for the 3rd International Conference on Bioscience and Biotechnology

2018. The conference will be held from 8th to 9th March under the theme “Pursuing

innovation in Bioscience and Biotechnology” .

The word "biotechnology" has received its importance and significance in last two decades,

which is just unprecedented. Probability and possibilities behind this kind of attention

towards biotechnology is due to its unlimited potential to serve and benefit humanity. It has a

significant impact in almost all the domains of human life, may it be health, environment,

food and agriculture.

Bioscience and Biotechnology 2018 depends on generous knowledge donations of experts to

promote the scientific discoveries. It will be committed to promote open, transparent, and

international scientific exchange on the “New research and innovations in Bioscience and

Biotechnology” and contribute to the improvement of efforts taken to tackle local and global

impacts of Biological Science.

The conference is organized as a set of main tracks; Application of Biochemistry and

Molecular Biology to Biotechnology, General Biological Engineering, Biomedical

Engineering and Bioinformatics, Computational Biology for Biotechnology and other related

tracks Biostatics, Biometric, Bio measurement, Biomechanics, Biophysics, and System

Biology. It focuses on importance of Bioscience and Biotechnology through the keynotes

speeches, Plenary speeches, invited talks, technical programs and workshops.

I hope this two days of scientific discussions and presentations by leading experts in the

World as well as new research carried out by young scientist will provide a perfect amalgam

for novel discovery in both basic and applied research in the Biotechnology. The outcome of

the conference will pave ways to many new developments in the field of Biotechnology

Worldwide. I am sure that you all will tremendously benefit from the deliberation of this

conference in a warm and friendly environment. Your presence and deliberation will make

this conference remarkably successful in all aspects of Biotechnology.

I congratulate all of you for your commitment and active participation and wish you all the

success.

Dr. Chamari Hettiarachchi

Conference Co –Chair BioTech 2018

Senior Lecturer in Molecular Biology and Biochemistry,

Department of Chemistry,

University of Colombo,

Sri Lanka.

ix

Table of Contents Page No

New Challenges for Mammalian Expression Systems and Cell

Engineering Strategies Applied to Overcome These

J. Budge

02

Molecular Mechanisms of Yeast Cell Wall Biosynthesis and How

Can We Use it to Engineer the Surface of the Cell

V. Mrša, A. Sk. Hossain, A. Grbavac, M. Lozančić M and R. Teparić

03

Arabidopsis to Rice: Applying Knowledge Gained from Model Plant

to Enhance Agriculturally Important Crop Plants

C. Hettiarachchi

04

A1 01 Development of E. coli Platforms for Diterpene Synthesis

M. Fuchs

06

A2

02 Functional and Structual Characterization of Setaria digitata Novel

Protein (SdNP)

M. Gowthami, K.Varmila, Gayathri. N. Silva and R.S. Dassanayake

07

A3 03 Analysis of Papillary Thyroid Carcinoma Associated Biomarkers and

Genetic Abnormalities to Determine the Risk of Developing Cancer

in Patients Diagnosed with Different Thyroid Disorders

P. Shirani, T. Dhanushika, F.I. Moulana, A.A.H. Priyani, M.C.V. de

Silva and R.S. Dassanayake

08

ORAL PRESENTATIONS

APPLICATION OF BIOCHEMISTRY AND MOLECULAR BIOLOGY TO

BIOTECHNOLOGY (A)

KEYNOTE ABSTRACTS

x

A4 04 Behavioral and Biochemical Effects of Banana (Musa sapientum L)

Peel Extract Following Noise Stress in Male Mice

N. Samad and A. Muneer

09

A5 05 Phytoremediation Potential of Legume Plant Cajanus cajan (Pigeon

Pea) on Petroleum Oily Sludge Contaminated Soil

I. A. Allamin, S.A. Ahmad, N.A. Yasid, S.R.S. Abdullah, M.I.E. Halmi

and M.Y. Shukor

10

A6 06 Response Surface-Based Optimization and Kinetic Studies of Phenol

Degradation by Alcaligenes sp. AQ5-02

A. Aisami, N.A. Yasid, W.L.W. Johari, S.A. Ahmad and M.Y. Shukor

11

A7 07 Isolation and Identification of Cadmium Sensing Regulator – cadC

from Bacillus megaterium strain TWSL-4

W.M.N.H. Kumari, C.D. Wijayarathna and N.V. Chandrasekharan

12

A8 08 Efficient Heterotrophic Mangrove Bacteria for Carbon Sequestration

through Microbially Induced Calcium Carbonate Precipitation

(MICP)

N.V. Kumar, P. Ammini and J. Vijayan

13

A9 09 siRNA Mediated RNA Interference of Novel Parasitic Nematode-

Specific Protein of Setaria digitata in Culex quinquefasciatus

Showed Molting and Movement Detects and Development Arrest

M.B.C.L. Somarathne

14

A10 10 High Throughput Screening of Bioactive Secondary Metabolites in

Sri Lankan Medicinal Plants by Microfractionation and Targeted

Isolation of Antibacterial Flavonoids

S. Rajendran, S. Mohotti, T. Muhammad, A.A. Strömstedt, A.

Adhikari, E.D. de Silva, U. Göransson, C.M. Hettiarachchi and S.

Gunasekera

15

A11 11 Study of Spring Wheat Varieties for Identification of Forms Resistant

to Cadmium

R.A. Alybayeva, M.F. Mulyukova, A.E. Bektiyar, V.I. Kruzhayeva,

S.D. Atabayeva and S.Sh. Asrandina

16

xi

A12 12 Antioxidant and Glycemic Regulatory Properties of Blue Pea

(Clitoria ternatea L.) Flower Extract

S.A.T. Lakshan, N.Y. Jayanath, W.P.K.M. Abeysekera and W.K.S.M.

Abeysekera

17

A13 13 Enhanced Production, Purification and Characterisation of Industrial

Enzyme by Penicillium lilacinum (Thom, 1910)

K. Ahmed

18

A14 14 Polyamine and Mycorrhiza Based Mitigation of Cadmium Toxicity

in Sorghum vulagre L.

K. Prasann and D. Padmanabh

19

B1 15 Characterization of Plant Growth-Promoting Bacteria (PGPB)

Isolated from the Rhizosphere of Alfalfa (Medicago sativa L.)

M.M.A. Khan , A. El Omri , S.A. Kabli , A. Bahieldin , and S.M.S. Al-

Garni

20

B2 16 Environmental Risk Factors for Bacteriological Contamination in

Urban Drinking Water Wells in Jaffna Municipal Council Area

S.Sivathasan, S. Saravanan and K.Gunalan

21

C1 17 In Silico Construction and Structure Validation of Humanized

Aldolase Catalyitic Antibody 38C2

J.A.L.I. Sampath, A.N.L. Japahuge and R.S. Jayakody

22

C2 18 iOMICS: New Paradigm in Algal Biotechnology

P.P. Jutur

23

GENERAL BIOLOGICAL ENGINEERING (C)

(C

BIOMEDICINE ENGINEERING (B)

(

xii

C3 19 Whether Mankind Has a Chance of Succour from the Plant Kingdom

in Neurodegeneration? An In-Silico Investigation

S. Thiruchittampalam and S. Weerasinghe

24

C4 20 Proving the Efficacy of Mangiferin as a Neuroprotective Drug Using

Docking Studies

P. Singh, R. Samadarsi and D. Dutta

25

C5 21 Identification and In-Sillico Characterization of a Novel Hindiii

Isoschizomer from a Pseudomonas spp

S.N.J. Pathirana, M. Kajan, D.A.S. Elvitigala, G.H.C.M.

Hettiarachchi, C.M. Nanayakkara, R.G.S.C. Rajapakse and N.V.

Chandrasekharan

26

P1 22 Determination of Desirable Attributes of a Member of Burkholderia

cepacia Complex as a Biological Control Agent of Plant Pathogenic

Fungi

V.N.D. Bulathsinhalage and D.M. De Costa

28

P2 23 Molecular Characterization of Genus Puntius Using Mitochondrial

Gene Markers from Rivers of Southern Western Ghats, India

A.A. Arunkumar

29

P3 24 Development of Microbial Inoculum to Enhance the Degradation of

Organic Waste

J.K.A. De Silva, G.A.U. Jayasekera and C.M. Nanayakkara

30

P4 25 Enhanced Production of Bacillus thuringiensis Subspecies israelensis

Delta Endotoxin by Supplementation of Rotten Mango Juice and

Fish-Amino Acid as Medium Supplements

C. Gopinathan

31

P5 26 Rheological Properties and Antioxidant Activity of High Pressure

Processed Citrus Juices

S. Karlović, T. Bosiljkov, F. Dujmić, F. Troha, M. Škegro, M. Marelja,

32

POSTER PRESENTATIONS (P)

xiii

M. Brnčić and D. Ježek

P6 27 Benign Fabrication of Copper Nanoparticles from Flower Extract of

Cassia auriculata L

G.P. Chandran, T. Priyadharshini, C. Rajkuberan, N. Sekar and D.S.

Kumar

33

P7 28 0

2

Biosynthesis of Silver Nanoparticles Using Leaf Extract of Zingiber

officinale (Ginger), Allium cepa var. Aggregatum (Shallot), Capsicum

annuum (Bird’s eye chili) and Curcuma longa (Turmeric):

Determination and Comparison of Antioxidant, Antimicrobial

Properties with Leaves Water Extraction

Y.D.B. Peramuna and M. Kandiah

34

P8 29 An Iridoid Glycoside from the Sri Lankan Medicinal Plant

Wendlandia bicuspidata

S. Rajendran, S. Mohotti, E.D. de Silva, U. Göransson, S. Gunasekera

and C.M. Hettiarachchi

35

P9 30 The Application of Surfactants in Volatile Organic Compound (VOC)

Removal

L. Haddad, Z. Shareefdeen and H. Ahmed

36

P10 31 The Study of Genetic Variability in Related to Efficiency of Zinc

Optake in Different Genotypes of Triticum Aestivum L

A. H. Ghorttapeh, A. Majidi, J. Mozafari and A. Zyaeian

37

32 Stock Identification and Some Biological Aspects of Frigate Tuna

(Auxis thazard) in Sri Lankan Waters

D.R. Herath, U. Seneviratne, H.A.C.C. Perera and G.H.C.M.

Hettiarachchi

39

VIRTUAL PRESENTATIONS

xiv

33 Production of Protein Hydrolysates from Fish Skin for Dairy Products

E.I. Titov, G.V. Semenov, I.I. Ionova, I.S. Krasnova and N.S. Kozlov

40

34 Influence the Parameters of Freeze Drying on Antioxidant Activity of

Yoghurts with Different Tropical Fruits

G.V. Semenov, I.I. Ionova, Z.V. Volokitina and I.S. Krasnova

41

35 Influence of High Hydrostatic Pressure on Physical Properties of

Apple Juice

T. Bosiljkov, K. Klas, S. Karlović, F. Dujmić, M. Škegro, M. Marelja,

M. Brnčić and D. Ježek

42

36 Degradation of 2,4,6 TNT by Native Microorganisms Isolated from

TNT Contaminated Soil

M. Devasena and A. Thenmozhi

43

37 Multi Features Clustering Approach to Determine Parkinsonian

Disease upon Gait Movement Artifacts

S. Anujan and M. S. Karunarathne

44

38 Ultrasonic - Assisted Extraction of Bioactive Compounds from Waste

in Production of Red Wine

F. Dujmić, S. Karlović, T. Bosiljkov, F. Troha, M. Škegro, M. Marelja,

D. Ježek, R. Vidrih and M. Brnčić

45

The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)

Page | 1

KEYNOTE ABSTRACTS


Page | 2

NEW CHALLENGES FOR MAMMALIAN EXPRESSION SYSTEMS AND CELL

ENGINEERING STRATEGIES APPLIED TO OVERCOME THESE

J. Budge

University of Kent, United Kingdom

ABSTRACT

Recombinant biotherapeutic protein production from cultured mammalian cells has become an

established technology in the last 30 years. Adalimumab (Humira) was the first humanized

monoclonal antibody molecule generated using a mammalian host to attain FDA approval (tPA was

first biotherapeuitc with approval made in mammalian cells) and, since then, mammalian cells have

been employed to produce an array of different recombinant biotherapeutic protein molecules.

Chinese hamster ovary (CHO) cells have become the most widely used industrial mammalian host to

generate biotherapeutic proteins as they are robust and capable of folding, assembling and secreting

complex, multi-domain molecules with human-like post translational modifications. However, 18 of

the top 20 biggest selling recombinant therapeutics in 2014 attained FDA approval over a decade ago

and parts of the commercial focus of the industry is moving away from the development of single

high selling products and towards more bespoke drug discovery processes and subsequent synthesis.

Furthermore, many of the molecules on the market are monoclonal antibodies but an increasing

number of novel format molecules are being developed that bring about new challenges for previously

established host cell lines. Thus, in order to address the demands of a dynamically changing market it

is important to establish expression platforms necessary for both drug discovery, and subsequently

manufacturing, processes. Presented here is an approach to improve transient gene expression through

improved DNA delivery into CHO cells, and in combination with the induction of an extra

chromosomal maintenance system, achieve transient yields greater than either individually. It is

equally important to develop production platforms which have the capacity to generate challenging,

novel format molecules. Also presented here is a cell engineering approach which has proved

successful in improving yields and product quality of difficult-to-express molecules in CHO cells.


Page | 3

MOLECULAR MECHANISMS OF YEAST CELL WALL BIOSYNTHESIS AND

HOW CAN WE USE IT TO ENGINEER THE SURFACE OF THE CELL

V. Mrša, A. Sk. Hossain, A. Grbavac, M. Lozančić M and R. Teparić

Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of

Zagreb, Croatia

ABSTRACT

Exploring microbial cell walls and particularly molecular mechanisms of wall protein localization

opened new possibilities of surface display of homologous and heterologous proteins. By fusing

anchoring domains of surface proteins with biotechnologically interesting proteins their genetic

immobilization is achieved. Hybrid proteins are expressed in the host cells, secreted to the cell surface

and incorporated into the cell wall. In this way chemical immobilization of proteins to a solid matrix

is avoided by using production cells as matrices themselves. Both bacteria and yeasts have been used

for this purpose and a number of potential biotechnological applications of surface displayed proteins

from whole cell biocatalysts, to biosorbents and biosensors have been reported. When surface

immobilized enzymes are used, substrates do not need to cross membrane barriers, i.e. enzymes can

freely access any externally added substrate. Thus, enzyme purification is bypassed and multi-step

transformations can be achieved using cells displaying enzymes catalyzing cascade reactions.

Particularly interesting are yeast systems for surface display since most yeasts are GRAS

microorganisms, their cell walls bind more proteins, and cells are bigger. Besides, yeasts are generally

more suitable for expression of proteins from higher eukaryotes. However, surface display in yeast

cells is still limited by our comprehension of mechanisms of cell wall biosynthesis and by insufficient

understanding of their regulation. An overview of yeast cell walls biosynthesis, as well as the

regulatory mechanisms involved will be presented. Applications of gained knowledge in the

development of the surface display technology will be discussed on several examples.

Keywords: yeast cell walls, cell wall biosynthesis, surface display, genetic immobilization


Page | 4

ARABIDOPSIS TO RICE: APPLYING KNOWLEDGE GAINED FROM MODEL

PLANT TO ENHANCE AGRICULTURALLY IMPORTANT CROP PLANTS

C. Hettiarachchi

Department of Chemistry, University of Colombo, Sri Lanka

ABSTRACT

Light is of utmost importance to sessile plants, not only as the primary energy source for

photosynthesis, but also as an environmental signal, regulating their growth and development.

CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) and ELONGATED HYPOCOTYL 5

(HY5) are two key regulators of plant light signaling pathway. COP1, an E3 ubiquitin ligase,

represses light dependent development by targeting positive regulators such as the transcription factor

HY5 for degradation in the dark. BBX21/ SALT TOLERANCE HOMOLOG 2 (STH2) is a B-box

encoding protein that interacts with both COP1 and HY5 in photomorphogenesis. BBX21 protein can

activate light dependent transcription and positively regulate the light mediated development of

plants.

It has been shown; the productivity of crop plants might be enhanced by over-expressing central

regulators of light signaling pathway. Bg 250 Sri Lankan rice variety was transformed with

Arabidopsis BBX21 gene via Agrobacterium mediated transformation to improve its quality and the

productivity. The T0 Bg 250 putative transgenic plants were screened for the presence of BBX21

gene by PCR and the PCR positive rice plants were further grown to obtain seeds. Transcript analysis

was conducted with homozygous T3 BBX21 transgenic plants and the results showed strong

expression of BBX21 gene in transgenic BG 250 plants compared to wild type Bg 250 plants.

Phenotypic studies of BBX21 T3 plants showed increased plant height, leaf length, leaf area, number

of panicles and bushy appearance due to increased number of tillers when compared with non-

transgenic plants.

Light stimulates germination of Arabidopsis seeds whereas the phytohormone Abscisic acid (ABA)

inhibits germination. BBX21 is a positive regulator of the light signals and appear to act as a negative

regulator of ABA signals suggesting that this protein might integrate these opposing signaling

pathways in plants. Genetic and expression analysis of double mutants between bbx21 and four key

regulators of ABA signaling showed that the expression of key regulators of ABA signaling is altered

in the bbx21 mutant indicating that the BBX21 gene plays an important role in response to drought

and salinity. At present research is being conducted to apply this knowledge to obtain drought and

salinity tolerant Bg 250 that could give higher yields per Hectare per year.


Page | 5

ORAL PRESENTATIONS


Page | 6

A1 [01]

DEVELOPMENT OF E. Coli PLATFORMS FOR DITERPENE SYNTHESIS

M. Fuchs

Technical University of Munich, Germany

ABSTRACT

The production of terpenoide drugs is often performed by a chemical (semi-)synthetic process

resulting in toxic chemical waste streams. Our research focuses on the development and optimization

of microbial production platforms for structurally minimized derivatives or artificially designed

compounds for pharmaceutical and agrochemical applications. Our processes do not affect land use

and are free of toxic waste streams. Hitherto, we have established a recombinant E. coli strain

carrying MEP pathway derived genes for IPP/DMAPP biosynthesis, which can be expanded with

different terpene cyclases. Based on molecular modeling terpene cyclases are engineered for the

production of new ring systems to get new compounds not only with anticancer but also with

antibiotic or anti-inflammatory effects. The taxadiene synthase, which forms the first dedicated

intermediate in taxol biosynthesis, taxadiene was engineered to produce verticillene, which also has

reported anticancer activity. The new terpenoide ring systems were further functionalized for

enhanced biological efficiency. The hydroxylation via lipases or stereo selective hydroxylases are

established possibilities is to attach acceptor functionalities for downstream acetylation or

benzoylaton. This was performed in a one-cell process or on purified terpenoid skeletons. In iterative

steps a systems biology approach improved the production strain. Here the product isolation from

fermentation media is a challenging task. In proof-of-concept studies product removal by extend

application of known cleaning strategies was tested using stationary adsorbers and counter current

chromatography methods.

Keywords: E. coli, diterpene drugs, functionalization, purification


Page | 7

A2 [02]

FUNCTIONAL AND STRUCTUAL CHARACTERIZATION OF Setaria digitata

NOVEL PROTEIN (SdNP)

M. Gowthami1, K.Varmila1, Gayathri. N. Silva1 and R.S. Dassanayake1

1Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka

ABSTRACT

Wuchereria bancrofti, a human parasitic nematode, is the major causative agent of “lymphatic

filariasis”. Studying the parasitology of W. bancroft iis highly challenging due to the difficulties

inobtaining an adult parasite from the lymphatic system. Therefore, Setaria digitata, a bovine filarial

parasite is used as a model organism due to its close resemblance to W.bancrofti and other filarial

nematodes. Setaria digitata novel protein (SdNP) was identified from S. digitata, that is uniquely

expressed in the parasitic nematodes. Sequence analysis and bioinformatics studies done on SdNP

revealed the presence of two partial ATPase domains and potential sites for phosphorylation. The

research work presented here describes in-vitro functional and structural characterization of SdNP.An

enzyme coupled ATPase assay was used to detect the ATPase activity of SdNP and the results show

that SdNP hydrolyses ATP to ADP and inorganic Pi in a substrate-independent manner. Pro-Q

diamond phosphor protein dye was used to detect the phosphorylation state of the protein and the

results confirm that SdNPis a phosphor-protein. In addition, native-PAGE and gel-filtration

chromatography were carried out to analyze the oligomerization state of SdNP. The results obtained

show that SdNP is forming a stable oligomer (approximately a tetramer).Overall, the results confirm

that SdNP is a tetramer and a phosphoprotein that has the ability to bind and hydrolyze ATP. Studying

the structure and function of SdNP is of great importance because SdNP is only found in pathogenic

nematodes and can be used as a potential target to develop clade-specific drugs against lymphatic

filariasis.

Keywords: Setaria digitata, SdNP, ATPase activity, Oligomer, Phosphorylation


Page | 8

A3 [03]

ANALYSIS OF PAPILLARY THYROID CARCINOMA ASSOCIATED

BIOMARKERS AND GENETIC ABNORMALITIES TO DETERMINE THE RISK

OF DEVELOPING CANCER IN PATIENTS DIAGNOSED WITH DIFFERENT

THYROID DISORDERS

P. Shirani1, T. Dhanushika1, F.I. Moulana, A.A.H. Priyani 2, M.C.V. de Silva2 and

R.S. Dassanayake 1

1Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka

2Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka

ABSTRACT

Thyroid disorders such as multinodular goiter (MNG) and Hashimoto’s thyroiditis (HT) leading to

papillary thyroid carcinoma (PTC) is a subject of controversy that prevails in the scientific

community. This prospective study investigates correlation between these disorders and PTC by

analyzing NRASQ61R, which is one of the most prevalent point mutation in PTC, and also the

expression levels of thyroid-stimulating hormone receptor (TSHR), manganese superoxide dismutase

(MnSOD) and a receptor tyrosine kinase (c-RET) in microscopic foci with similar nuclear features for

PTC in patients with HT and MNG. A total of 22 formalin-fixed paraffin embedded (FFPE) tissue

samples were subjected to polymerase chain reaction-restriction fragment length polymorphism

(PCR-RFLP) to analyze NRASQ61R mutation, of which 18 FFPE samples were subjected to real-time

PCR relative quantification to analyze the gene expression levels in the test regions compared to the

selected control regions. In the detection of NRASQ61R mutation, 95.5% of samples tested were

positive for the mutation in both test and control areas. In the quantification of mRNA, 61% of

samples showed an elevation in TSHR levels, 78% in MnSOD levels while 50% in c-RET levels in the

test areas relative to the control areas. Elevation of mRNA levels suggests that there is a higher

potential of patients with MNG and HT to develop PTC. This is further supported by the presence of

NRASQ61R point mutation in most of the samples analyzed.

Keywords: MnSOD, c-RET, TSHR, NRASQ61R, PCR-RFLP, HT


Page | 9

A4 [04]

BEHAVIORAL AND BIOCHEMICAL EFFECTS OF BANANA (Musa sapientum L)

PEEL EXTRACT FOLLOWING NOISE STRESS IN MALE MICE

N. Samad and A. Muneer

Department of Biochemistry Bahauddin Zakariya University, Multan-Pakistan

ABSTRACT

The present study was aimed to investigate the anti-anxiety, antidepressant and memory enhancing

effects of banana (Musa sapientum L.) peel extract following acute noise stress in male mice. Locally

bred albino Wistar mice were divided into control and test groups (n=12). Control mice were received

drinking water while test groups were treated with banana peel extract (400 mg/kg; oral

administration). Animals were received their respective treatment for 14 days. Animals were

subdivided (n=6) into unstressed and stressed groups on day 15. Stressed groups were subjected to

noise stress for 4-h. Behavioral activities of animals were monitored 24-h after the 4-h noise stress.

Depression-like symptoms were measured by forced swimming test (FST). Anxiety like behavior was

monitored using light–dark transition test and plus maze activity test and memory functions of mice

were assessed by morris water maze (MWM) test. 1-h after the post administration of respective

treatment animals were decapitated and brain was removed for estimation of antioxidant enzymes

[catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH)], acetylcholinestrase

(AChE) and oxidative stress marker malondialdehyde (MDA). In the present study banana peel

attenuated stressed-induced behavioral deficits and produced anxiolytic and antidepressant-like

effects. An increase in memory performance was also observed in banana peel treated animals. The

activities of all antioxidant enzymes were significantly greater in banana peel treated unstressed and

stressed animals while activity of AChE is decreased in unstressed animals. Conversely, banana peel

extract reduced the levels of MDA in both unstressed and stressed groups than control animals. It is

concluded that banana peel have anti-anxiety, antidepressant effect as well as strengthen the memory

possibly via its antioxidant mechanism following acute noise stress. Therefore, it is recommended that

supplementation of banana peel could be beneficial for the treatment of anxiety, and depression and

improve memory function.

Keywords: Banana peel, noise stress, depression, anxiety, memory, antioxidant enzymes


Page | 10

A5 [05]

PHYTOREMEDIATION POTENTIAL OF LEGUME PLANT Cajanus cajan

(PIGEON PEA) ON PETROLEUM OILY SLUDGE CONTAMINATED SOIL

I. A. Allamin1,2, S.A. Ahmad1, N.A. Yasid1, S.R.S. Abdullah3, M.I.E. Halmi4 and

M.Y. Shukor1

1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia, Selangor, Malaysia

2Department of Microbiology, Faculty of Sciences, University of Maiduguri, Maiduguri,

Nigeria

3Department of Chemical and Process Engineering, Faculty of Engineering and Built

Environment, Universiti Kebangsaan Malaysia, Malaysia

4Department of Land Management, Faculty of Agriculture, University of Putra Malaysia,

Malaysia

ABSTRACT

A considerable amount of polluti is generated from petroleum industry during exploration,

production, transportation, storage, and refining processes. Most components of petroleum

hydrocarbon disposed or spilled to the environment are toxic, mutagenic and cancer-causing. There is

the need of effective and ecofriendly remediation measures. Thus, research in phytoremediation. C.

cajan a legume plant was screened on petroleum oily sludge contaminated soil. Plant tolerance to the

contaminant was monitored and determined (plant height, dry biomass and RGR). Rhizosphere soil

was sampled to determined (total heterotrophic bacteria (THB) using nutrient agar, nitrogen fixing

bacteria (NFB) using yeast extract mannitol agar, and hydrocarbon utilizing bacteria (HUB) using oil

agar). Degradation rates were estimated gravimetrically and quantified on GC-FID. Accumulation of

heavy metal and root exudates released by C. cajan was determined using AAS and HPLC

respectively. The response of the plant to oily sludge varied significantly to the untreated control and

among the treatments. The growth of C. cajan was slightly (but not significantly) influenced by the

oily sludge in soil; growth of C. cajan in relatively lower concentrations of petroleum oily sludge

slightly greater to the untreated control without petroleum oily sludge. A significant interaction was

observed for relative growth rates (RGRs) biomass of C. cajan. The removal of total petroleum

hydrocarbon (TPH) after 90 days in treatments with 1%, 2% and 3% shows 92%, 90% and 89%

respectively and 68.3% and 47.3% in relatively higher concentrations 4% and 5% respectively. As the

search for more eco-friendly and sustainable remediating green plant continues, C. cajan shows a

great potential in reclaiming petroleum oily sludge-contaminated soil. Our findings will provide

solutions to polluted soils and subsequent re-vegetation.

Keywords: Oily sludge, Cajanus cajan, phytoremediation and legume-microbe


Page | 11

A6 [06]

RESPONSE SURFACE-BASED OPTIMIZATION AND KINETIC STUDIES OF

PHENOL DEGRADATION BY Alcaligenes SP. AQ5-02

A. Aisami 1,2, N.A. Yasid1, W.L.W. Johari3, S.A. Ahmad1 and M.Y. Shukor1

1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia, Malaysia

2Department of Biochemistry, Gombe State University, Nigeria

3Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra

Malaysia, Malaysia

ABSTRACT

In Malaysia, phenol and phenolic compounds rank among the top three scheduled wastes with

thousands of tonnes being produced yearly for disposal. In Malaysia about 37.7 metric tonnes of

phenol and phenol-containing wastes are produced in 2014, there is also an incident of tanker

accidents Straits of Malacca in 2003 where tonnes of phenol were spilled. Bio-removal of phenol by

microorganisms especially bacteria has been demonstrated to be the most effective and economical

approach compared to physio-chemical methods. In this study, a phenol-degrading Alcaligenes sp.

AQ5-02 has been isolated from a contaminated area. Optimisation using Response Surface

Methodology showed that the highest degradation was achieved at pH 7.5 (phosphate buffer), 0.45

g/L ammonium sulphate and an optimum temperature at 30°C. Growth kinetic modelling of the

bacterium on phenol shows that the best model was Luong with the lowest values for root mean

square error or RMSE and adjusted Akaike Information criteria AICc, highest adjusted R2 values, and

with Bias Factor and Accuracy Factor nearest to unity (1.0). The calculated value for the Luong’s

constants maximal growth rate, half saturation constant for maximal growth, maximal concentration

of substrate tolerated and curve parameter that defines the steepness of the growth rate decline from

the maximum rate, symbolized by umax, Ks, Sm, and n were 0.07±0.01 hr-1, 0.18±0.03 g/L, 1.27±0.24

g/L and 6.60±0.94 for Alcaligenes sp. AQ05-02. Phenol was degraded through the meta pathway.

Keywords: Phenol, Alcaligenes sp., Response Surface Method, Luong, meta pathway


Page | 12

A7 [07]

ISOLATION AND IDENTIFICATION OF CADMIUM SENSING REGULATOR –

CADC FROM Bacillus megaterium STRAIN TWSL-4

W.M.N.H. Kumari, C.D. Wijayarathna and N.V. Chandrasekharan

Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka

ABSTRACT

In the search for an eco-friendly solution to decontaminate toxic heavy metals existing in industrial

effluents, a bacterial strain designated as TWSL-4 with metal ion (Cu2+, Cd2+ and Pb2+) resistance and

removal ability was isolated and identified up to the species level (B. megaterium) using 16S rRNA

gene analysis. Initial studies revealed the presence of genes involved in cadmium ion efflux

mechanism (P-type ATPases/cadA) in this bacterium. The current study was focused on finding genes

related to the cad operon. The upstream region of the cadA gene was amplified and cloned into a

pGMET vector. The 375 bp insert was sequenced. BLASTn analysis revealed the sequence to be 99%

homologous to the cadC gene (cds=119 aa) which codes for the Cadmium efflux system accessory

protein (CadC) of B. megaterium and 72% homologous to the cadC gene (cds=121 aa) of

Staphylococcus sp. BLASTp analysis showed 100% homology to ArsR family transcriptional

regulator of Bacillus sp. Protein sequences of both species shared similar features although protein

homology was around 70%. However the amino acids involved in binding at the ion binding site

(amino acids 57, 60 and 102) are conserved confirming functional similarity. Phylogenetic analysis of

the cadC gene revealed it to be highly related to the cadC gene of Bacillus sp. rather than

Staphylococcus sp. reflecting the BLAST search results. Overall results revealed the isolated gene to

code for cadmium sensing regulator with three sites; dimerization interface [polypeptide binding],

putative DNA binding site [nucleotide binding] and putative Zn2+ binding site [ion binding].

Keywords: heavy metals, industrial effluents, Bacillus megaterium, cad operon, cadC


Page | 13

A8 [08]

EFFICIENT HETEROTROPHIC MANGROVE BACTERIA FOR CARBON

SEQUESTRATION THROUGH MICROBIALLY INDUCED CALCIUM

CARBONATE PRECIPITATION (MICP)

N.V. Kumar, P. Ammini and J. Vijayan

National Institute of Oceanography-CSIR, Regional Centre, Dr. Salim Ali Road, India

ABSTRACT

Microbially induced calcium carbonate precipitation (MICP) is a natural bio-mineralization process

induced by microorganisms involving bacterial extracellular enzymes. Carbonic anhydrase and urease

are the major enzymes playing major role in the process of MICP. In this study, 15 bacterial colonies

(PV1-PV15) were isolated from mangrove sediments of Puthuvype, Kerala, India. The isolates were

screened for their carbonic anhydrase and urease enzyme production based on p-Nitro phenol acetate

assay and urease agar method respectively. The positive isolates were subjected to secondary

screening through quantitative method. Among them, a potential isolate was identified as

Virgibacillus halodenitrificans PV15 based on 16S rDNA sequencing. The isolate exhibited 40 U mL-

1 of carbonic anhydrase and 731.91 U mL-1 of urease activity. The enzyme kinetics was studied based

on Line weaver-Burk plots. The calcium precipitation assay to determine the carbon sequestration

ability of the microbial isolate showed a reduction of 72% of free calcium in the medium through

calcite precipitation. The carbonic anhydrase and urease had a synergistic effect in MICP. CA and

urease mediated calcium precipitation by V. halodenitrificans PV15 could contribute towards the

process of carbon dioxide sequestration. This isolate could be used for effectively for the carbon

dioxide sequestration application. Moreover, the enzyme mediated bio-mineralization is one of the

most economical methods for mitigating global warming.

Keywords: carbon sequestration, bacteria, carbonic anhydrase, urease, mangroves


Page | 14

A9 [09]

siRNA MEDIATED RNA INTERFERENCE OF NOVEL PARASITIC NEMATODE-

SPECIFIC PROTEIN OF Setaria digitata IN Culex quinquefasciatus SHOWED

MOLTING AND MOVEMENT DETECTS AND DEVELOPMENT ARREST

M.B.C.L. Somarathne

University of Colombo, Sri Lanka

ABSTRACT

Functional studies of animal parasitic nematodes at molecular level are being held back due to the

scarcity of available nematode culturing methods and gene manipulation tools. Therefore this study

was undertaken to utilize novel in-vivo culturing method in mosquito together with RNA interference

post transcriptional gene silencing tool in functional analysis of Setaria digitata nematode specific

gene (SDNP). Culex quinquefasciatus mosquitoes, the natural vector of the parasite were infected

with S. digitata microfilariae by micro-injection. RNAi treatment was executed with the introduction

of in-vitro synthesized Cy3 labeled siRNA in to the infected mosquitoes. Following the RNAi

treatment, red fluorescence was observed inside the larvae, indicating the uptake of siRNA. q-PCR

analysis demonstrated a significant reduction in the transcript (90% (P < 0.05) levels of SDNP in the

RNAi treated larvae compared to the non-treated, suggesting the RNAi pathways are operational in

the parasite. The resulted motility reduction, molting deformities (77% reduction in L2-L3

transformation) and the irregularities in the migration of the parasite in the mosquito followed by the

RNAi, pointed out the involvement of SDNP in locomotion and growth of the worm. Furthermore,

migration irregularities and increased mosquito survival in the test group eventually resulted in a

dramatic decrease in the parasitism of the larvae. Collectively, this study demonstrates the successful

utilization of mosquito as an in-vivo culture platform and RNAi as a robust tool in gene manipulation.

Moreover, according to the characteristics of SDNP, it can be suggested that it has a potent

candidature as a novel anthelminthic drug target.

Keywords: S.digitata, RNAi, in-vivo culturing, SDNP


Page | 15

A10 [10]

HIGH THROUGHPUT SCREENING OF BIOACTIVE SECONDARY

METABOLITES IN SRI LANKAN MEDICINAL PLANTS BY

MICROFRACTIONATION AND TARGETED ISOLATION OF ANTIBACTERIAL

FLAVONOIDS

S. Rajendran 1,2 , S. Mohotti1,2, T. Muhammad1, A.A. Strömstedt1, A. Adhikari3, E.D. de

Silva2, U. Göransson1, C.M. Hettiarachchi2 and S. Gunasekera1

1Division of Phamacognosy, Department of Medicinal Chemistry, Uppsala University,

Biomedical Centre, Sweden

2Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka

3International Centre for Chemical and Biological Sciences, H. E. J. Research Institute of

Chemistry, University of Karachi, Pakistan

ABSTRACT

Sri Lankan Ayurvedic system of traditional medicine derives from plant-based remedies handed down

from generation to generation over a period of 3000 years. Despite being an integral part of the Sri

Lankan culture, the scientific evidence for the biological activity of these traditional medicines are

limited and there’s a lack of scientific evidence for efficacy, toxicity, standardization of formulations

as well as adverse interactions of the medications. The current project was conducted to identify and

characterize chemical compounds present in selected Sri Lankan medicinal plants belonging to the

family Fabaceae, which have potential utility as therapeutic candidates for antimicrobial applications.

The medicinal plants were chosen based on their ethno pharmacology values. Organic crude extracts

of selected plants were subjected to agar disc diffusion assay against Staphylococcus aureus, Bacillus

cereus, Pseudomonas aeruginosaand Escherichia coli. Aqueous extracts of plantswere also subjected

to antibacterial activity screeningby a microfractionation protocol. Each aqueous crude plant extract

was fractionated into a 96 deep well micro titer plate using analytical HPLC (2 mg of crude extract to

obtain 45 x 1 ml fractions) and the fractions were screened against S. aureusin an optimized Minimum

Inhibitor Concentration assay. The corresponding masses in the bioactive microfractions were then

identified by liquid chromatography coupled to mass spectroscopy.Among the tested plants, Derris

scandens showed the highest activity against Staphylococcus aureus. D. scandenswas then subjected

to aactivity/mass guided fractionation protocol to isolate corresponding masses in the bioactive

fractions; four pure flavonoids (Dalpanitin, Rutin, Vicenin2 and Vicenin3) were isolated and their

structures were elucidated by NMR. Pure compounds were also subjected to biological assays in order

to confirm their antibacterial activity.

Keywords: Derris scandens, microfractionation, Traditional medicine, MIC, LCMS


Page | 16

A11 [11]

STUDY OF SPRING WHEAT VARIETIES FOR IDENTIFICATION OF FORMS

RESISTANT TO CADMIUM

R.A. Alybayeva, M.F. Mulyukova, A.E. Bektiyar, V.I. Kruzhayeva, S.D. Atabayeva and

S.Sh. Asrandina

Kazakh National University named after al-Farabi, Almaty, Republic of Kazakhstan

ABSTRACT

The purpose of our study was the identification of spring wheat varieties resistant to cadmium, the

priority pollutant in the East Kazakhstan region. The experiments were carried out in the condition of

model contamination of the medium on 14-day sprouts of various varieties of spring wheat from the

collection of the Kazakh Research Institute of Farming and Plant Growing grown on a nutrient

mixture containing 0.1 mM CaSO4 and Cd ions at a concentration of 40 mg/l (as a CdSO4 salt).

Screening of varieties of spring wheat under laboratory conditions made it possible to identify stable

and sensitive genotypes. In terms of growth, accumulation of cadmium in the aerial organs the most

resistant to cadmium are the varieties of spring wheat Samal and Kayyr, the least resistant are the

varieties Lutescens and Zhenis. In terms of growth, accumulation of cadmium in the roots the most

resistant varieties were spring wheat Kazakhstanskaya-15 and Kazakhstanskaya Early, the most

unstable - the variety Lutescens. A slight increase in the maintenance of chlorophyll a and b in the

presence of cadmium in most varieties was revealed. The fact of a smaller variability in the ratio of

a/b chlorophylls in more resistant to cadmium translocation to the aerial organs of genotypes is

revealed, compared with more sensitive ones. Cadmium-resistant varieties can be used in field studies

of soil contamination with cadmium to identify promising forms that combine metal resistance with

high yield and resistance to weather conditions.

Keywords: cadmium, spring wheat, growth parameters, cadmium content, chlorophyll content,

sensitive and resistant genotypes


Page | 17

A12 [12]

ANTIOXIDANT AND GLYCEMIC REGULATORY PROPERTIES OF BLUE PEA

(Clitoria ternatea L.) FLOWER EXTRACT

S.A.T. Lakshan1, N.Y. Jayanath2, W.P.K.M. Abeysekera3 and W.K.S.M. Abeysekera4

1,2University of Peradeniya, Sri Lanka

3Industrial Technology Institute, Sri Lanka

4University of Colombo, Sri Lanka

ABSTRACT

Flower of Clitoria ternatea is reported to have various biological activities world over. However,

there is no single study conducted in Sri Lanka on biological activities of this plant. This study

investigated antioxidantand glycemic regulatory properties of Clitoria ternatea flower. Hot water

extract of Clitoria ternatea blue flower was used in this study. Pre-optimized conditions of

temperature (59.6 ºC), time (37 min), and flower: water ratio (3 g/L) were used in extraction. In- vitro

antioxidant properties were investigated in terms of total phenolic content (TPC), total flavonoid

content (TFC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity

(ORAC) and DPPH and ABTS+ radical scavenging activities. Glycemic regulatory properties were

investigated using in vitro anti-amylase and anti-glucosidase assays. TPC, TFC, FRAP, ORAC, DPPH

(IC50) and ABTS+(IC50) radical scavenging activity antioxidant properties of the flower extract were

26.72±2.17 mg gallic acid equivalents/g, 14.25±0.58 mg quercetin equivalents/g, 5.13±0.54 mg trolox

equivalents (TE)/g, 36.73±1.06 mg TE/g, 45.31±1.47 μg/mL, and 6.74±0.10 µg/mL respectively.The

observed antioxidant properties were moderate compared to the reference standards used. Inhibition

of α-amylase by the flower extract was low (4.28±1.02 %) whereas no anti-glucosidase activity was

observed at the tested concentrations. In conclusion, the water extract of blue Clitoria ternatea flower

had antioxidant properties via various modes of actions with mild anti-amylase activity.

Keywords: Antioxidants, Glycemic regulatoryproperties, Blue pea flower, Hot water extract


Page | 18

A13 [13]

ENHANCED PRODUCTION, PURIFICATION AND CHARACTERISATION OF

INDUSTRIAL ENZYME BY Penicillium lilacinum (THOM, 1910)

K. Ahmed

Department of Chemistry, NED University of Engineering & Technology, Karachi, Pakistan

ABSTRACT

Recent years researchers have been motivated towards extensive exploring of living organism, which

could be utilized effectively in intense industrial conditions. The present study shows enhanced

production, purification and characterization of industrial enzyme, invertase (Beta-D-

fructofuranosidase) from Penicillium lilacinum (Thom, 1910). Various agricultural based by-products

(cotton stalk, sunflower waste, rice husk, molasses and date syrup) were used as energy source. The

highest amount of enzyme (13.05 Units/mL) was produced when the strain was cultured on growth

medium containing date syrup as energy source, yeast extract as a source of nitrogen, after 96 h of

incubation, at incubation temperature 40º C, initial pH of medium 8.0, inoculum size of 6x106 conidia

and 200 rev/min of agitation rate. The enzyme was also purified (7 folds than crude) and

characterized. Molecular mass of purified enzyme (65 kDa) was determined by 10 % SDS-PAGE.

Lineweaver-Burk Plot was used to determine Kinetic constants (Vmax 178.6 U/mL/min and Km 2.76

mM). Temperature and pH optima were 55º C and 5.5 respectively. MnCl2 (52.9 %), MgSO4 (48.9

%), BaCl2 (24.6 %), MgCl2 (9.6 %), CoCl2 (5.7 %) and NaCl (4.2 %) enhanced the relative activity of

enzyme and HgCl2 (-92.8 %), CuSO4 (-80.2 %) and CuCl2 (-76.6 %) were proved inhibitors. The

strain was showing enzyme activity even at extreme conditions of temperature (up to 60º C) and pH

(up to 9) so it could be potential candidate for industries.

Keywords: Invertase, Penicillium lilacinum, Submerged fermentation, Industrial enzyme


Page | 19

A14 [14]

POLYAMINE AND MYCORRHIZA BASED MITIGATION OF CADMIUM

TOXICITY IN Sorghum vulagre L.

K. Prasann1 and D. Padmanabh2

1Department of Agronomy, School of Agriculture, Lovely Professional University, India

2Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu

University, Varanasi, India

ABSTRACT

Cadmium is one of the most hazardous heavy metals in the atmosphere, soil and aquatic system which

is finally going into our food chain and responsible for serious environmental problem leading to the

health hazards in the living organisms. Sorghum vulgare L. plants when exposed to cadmium nitrate

with the concentrations of 70ppm and 150ppm per kg of soil for 90 days, exhibited phytotoxic

responses. The observations of specific responses were dependent on treatment combinations. The

significant hazardous effects and oxidative damage of cadmium nitrate (70 ppm and 150ppm) were

evident by increased MDA content and hydrogen peroxide content. The metal tolerance and

detoxification strategy adopted by the plant was investigated with reference to a non enzymatic

antioxidant system (proline) and the antioxidant enzymes viz., Superoxide dismutage, Polypheol

oxidase, Peroxidase and catalase, which were found to be reduced due to cadmium stress. However,

these responses were reversed by exogenous application of putrescine (2.5 and 5.0 mM) and

mycorrhiza (Glomus; 150 inoculants per kg of soil), more so, in their combined treatment, at different

DAS. Peaks of alcoholic, aldehyde and ketone functional groups were sharper and strong in plants

grown in higher concentration of cadmium after the observation under FT-IR. Whereas plants treated

with putrescine and Glomus showed a decreasing trend of sharpness of peaks with respect to non

treated plants. Dispersion Scanning Calorimetric data showed decreasing trend of stability with

increasing concentration of cadmium. This was expressed in the terms of glass transition phase

temperature. But combined treatment of putrescine and mycorrhiza enhanced the stability of sorghum

by reducing the ROS production in plant cell. On the basis of the data obtained, it is concluded that

plants responded up to 70 ppm cadmium nitrate with stress induced responses which were ameliorated

by combined application of putrescine and Glomus mycorrhiza.

Keywords: agriculture, biotic, cadmium, density, energy


Page | 20

B1 [15]

CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA (PGPB)

ISOLATED FROM THE RHIZOSPHERE OF ALFALFA (Medicago sativa L.)

M.M.A. Khan 1, A. El Omri 1, S.A. Kabli 1, A. Bahieldin 1, and S.M.S. Al-Garni 1

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Saudi

Arabia

ABSTRACT

Application of plant growth-promoting bacteria (PGPB) is an environmentally-friendly approach to

enhance crop production. However, indigenous PGPB are likely to be more adaptive and may serve as

better plant growth promoters. In this study, twenty five bacteria were isolated from the rhizosphere of

alfalfa plant growing in Jeddah, Saudi Arabia, and assessed for indole-3-acetic acid (IAA), 1-

aminocyclopropane-1-carboxylic acid (ACC) deaminase, and inorganic phosphate (Pi) solubilization

activity in vitro. Out of 25 bacteria, ten isolates produced significant amount of IAA in presence of L-

tryptophane. The maximum (85 μg/ml) IAA was synthesized by KB-10. Only KB-25 displayed all the

three tested PGP traits i.e., IAA (19 μg/ml), ACC deaminase (0.1 μmol α-ketobutyrate/mg/h), and Pi

solubilization activity. These two strains were further tested for exopolysaccharides, ammonia, and

hydrogen cyanide (HCN) production ability in vitro. Both KB-10 and KB-25 were found positive for

ammonia, but negative for exopolysaccharides and HCN production. They were gram-negative, rod-

shaped, motile, catalase and oxidase positive. The 16S rRNA genes of KB-10 and KB-25 showed

100% sequence homology with Pseudomonas pseudoalcaligenes and P. putida, respectively. Based

on their phenotypic, biochemical and 16S rRNA gene phylogeny, KB-10 and KB-25 were tentatively

identified as P. pseudoalcaligenes KB-10 and P. putida KB-25, respectively. Currently, a pot

experiment is going on to evaluate the effectiveness of these strains to improve growth and

productivity of alfalfa in nutrient-impoverished soil. This study reports two PGPB strains native to

Saudi Arabia which may have the potential to be used as bioinoculants for sustainable alfalfa

cultivation.

Keywords: PGPB, IAA, ACC deaminase, Pseudomonas, alfalfa


Page | 21

B2 [16]

ENVIRONMENTAL RISK FACTORS FOR BACTERIOLOGICAL

CONTAMINATION IN URBAN DRINKING WATER WELLS IN JAFFNA

MUNICIPAL COUNCIL AREA

S.Sivathasan1, S. Saravanan2 and K.Gunalan3

1,2National Water Supply and Drainage Board, Jaffna, Sri Lanka

3Deparment of Fisheries, University of Jaffna, Sri Lanka

ABSTRACT

The Jaffna Municipal Council area (JMC) lies in the Southern part of Jaffna Peninsula. The Jaffna

lagoon forms the Southern boundary of the area. The JMC consists of Jaffna Divisional Secretariat

Division and part of Nallur Divisional Secretariat Division. The objective of the study was to define

the presence of bacterial contamination of Ground water used for domestic purposes in Jaffna

Municipal council area. Ninety one well locations were selected to represent the JMC area water

quality, and the locations include domestic and common dug wells and analyzed in December 2014 to

February 2015 (during the raining seasons) and results were compared with Sri Lankan drinking water

quality standard (SLS 614:2013). The results showed of bacterial contamination in the water sources

means that some forms of treatment needed to be done before consumption. The mean count of total

coliform and faecal coliform ranged from 0 to 705 CFU/100 mL and 0 to 637 CFU/100 mL water

sample, respectively. Most of costal area brackish water wells from Gurunagar, Paasiyoor,

Navanthurai and Colombothurai also showed high fecal contamination. The Jaffna Town does not

have proper sewerage facilities and every household are having individual toilet with a open dug pit.

These open pits are the source of contaminant for the bacterial pollution. The most basic and

important factor is the short distance / setback distances between the source of pollution and the well.

Every household have individual well because of the non-availability of pipe born water supply and

most of the people are using the water for their day to day needs as well as Drinking water. For

adequate determination of setback distances, information on attenuation and transport of pathogens or

microbial indicators in groundwater systems are needed. In the larger interests of public health, the

implementation of a sewerage scheme for the city is absolutely necessary.

Key words: E.coli, T.coli, Jaffna Municipal Council


Page | 22

C1 [17]

In Silico CONSTRUCTION AND STRUCTURE VALIDATION OF HUMANIZED

ALDOLASE CATALYITIC ANTIBODY 38C2

J.A.L.I. Sampath, A.N.L. Japahuge and R.S. Jayakody

Department of Chemistry, University of Sri Jayewardenepura, Sri Lanka

ABSTRACT

Catalytic antibodies are immunoglobulin proteins that have catalytic activity similar to enzymes.

However, unlike enzymes abenzymes are capable of accepting a wide variety of substrates. Aldolase

catalytic antibody 38C2 catalyzes aldol and retro-aldol reactions via enamine mechanism. Main

features of abenzyme 38C2 are substrate orthogonality to natural aldolase and wide range of substrate

tolerance. Therefore, 38C2 has been used in selective prodrug activation and thereby, developed as a

tool for selective chemotherapy. However, its 3D atomic structure is not known yet. In this study, it

was attempted to construct the 3D atomic structure of humanized abenzyme 38C2 using its primary

amino acid sequence. Modeling was carried out by principles of Homology modeling. The3D atomic

structure of abenzyme 33f12 (PDB ID: 1AXT) was used as the template for the model construction

which shares 80% amino acid sequence identity. The accuracy of the constructed model was

evaluated using Ramachandran plot and the scoring function Qmean. Further, Dynamic stability was

evaluated using molecular dynamic simulations. Binding of natural substrate, acetone, to the novel

structure was studied using automated molecular docking. Docked complex’s dynamic stability was

observed from molecular dynamic simulations. Finally, the first step of the catalytic reaction has been

successfully carried out with IRC/QST3 calculations with DFT/B3LYP-6-31G level of theory in the

extracted catalytic residues in implicit water. This study concluded that the 3D structure of humanized

abenzyme 38C2 has successfully constructed and validated. The novel structure can be used to

enhance its application in drug discovery.

Keywords: Abenzyme, Aldolase,Homology modelling


Page | 23

C2 [18]

iOMICS: NEW PARADIGM IN ALGAL BIOTECHNOLOGY

P.P. Jutur

International Centre for Genetic Engineering and Biotechnology, New Delhi, India

ABSTRACT

Decreasing fossil fuels and its impact on global warming has led to an increasing demand for its

replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for

renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valuable

biofuels, which is of great importance for the food and energy industries. Parachlorella kessleri, a

marine unicellular green alga belonging to class Trebouxiophyceae, accumulates large amounts of oil

i.e., lipids under nutrient-deprived (-N, -P, and -S) conditions. Understanding their genomic and

metabolic imprints is important for elucidating the physiological mechanisms of lipid accumulations

in this microalga subjected to nutrient deprivation. One of feed-backward approach using metabolic

and lipidomic profiles by gas chromatography-mass spectrometry (GC-MS) of P. kessleri under

nutrient starvation (-N, -P and -S) conditions shown relative quantities of more than 100 metabolites

were significantlyexpressed compared in all these three starvation conditions. Our results demonstrate

that in lipid metabolism, the quantities of neutral lipids increased significantly followed by the

decrease in other metabolites involved in photosynthesis, nitrogen assimilation etc. In conclusion, the

metabolomics and lipidomic profiles have identified a few common metabolites such as citric acid,

valine and trehalose to play a significant role in the over production of oil by this microalga subjected

to nutrient deprivation. Understanding the entire system through iOmics (integrated omics) profiling

will lead to identifying relevant metabolic pathways involved in the biosynthesis and degradation of

precursor molecules that may have the potential for biofuel production, aiming towards the vision of

tomorrow’s bioenergy needs.

Keywords: algae, biofuels, omics, synthetic biology


Page | 24

C3 [19]

WHETHER MANKIND HAS A CHANCE OF SUCCOUR FROM THE PLANT

KINGDOM IN NEURODEGENERATION? AN In-Silico INVESTIGATION

S. Thiruchittampalam and S. Weerasinghe

Department of Chemistry, University of Colombo, Sri Lanka

ABSTRACT

Neurodegenerative diseases, Alzheimer’s (AD), Parkinson’s (PD), prion and Huntington’s (HD)

diseases afflicting large number of the population worldwide have been consolidated aiming

advancement of therapy simultaneously due to the causal tie-ups such as oxidative stress, protein

misfolding and aggregation. Most drugs that have been tried against these disorders cannot stop the

progression of the diseases and the chronic usage of the drugs enervates sufferers and caregivers due

to the abrupt side effects. Since the critical involvement of accumulation of certain metal ions in

oxidative stress, protein misfolding and aggregation, chelation therapy could be urged as a sensible

therapeutic remedy for neurodegeneration. Therefore, suitable molecules for the diseases related metal

ions Fe(III), Cu(II), Zn(II) and Al(III) were identified, computationally, to be used as chelating agents

from the database (http://scienc.cmb.ac.lk/tools/slflora) of ~200 molecules of native plants and

endemic plants of Sri Lanka, envisioning minimum side effects. The stability of metal-ligand

complexes was determined by calculating free energies of binding from the free energy landscape

along the reaction coordinate resulted as the upshot of umbrella sampling molecular dynamics

simulations. The simulation findings spots therapeutic potential compounds from 12 plants for AD,

PD and prion disease. Moreover, there is no potential compound to assort for the HD.

Keywords: neurodegeneration, chelation therapy, in-silico, free energy


Page | 25

C4 [20]

PROVING THE EFFICACY OF MANGIFERIN AS A NEUROPROTECTIVE DRUG

USING DOCKING STUDIES

P. Singh, R. Samadarsi and D. Dutta

Department of Biotechnology, National Institute of Technology Durgapur, West Bengal,

India

ABSTRACT

Mangiferin, a bioactive Xanthonoid known for its anti-oxidizing& therapeutic nature, has been

involved in a variety of potential pharmacological activities. The aim of this work is to prove the

efficacy of mangiferin as a neuroprotective drug by docking it with β-amyloid precursorprotein which

causes Alzheimer’s. Alzheimer’s disease (AD) is a progressive neurodegenerative disease which is

characterized by progressive cognitive deterioration and behavioral changes along with the chronic

inflammation of neurons. Studies have shown that the deposition of amyloid-β (Aβ) peptide have

resulted in the inflammatory changes such as astrocytosis and microgliosis. Based on the quantitative

analysis of the molecular docking technique it was observed that mangiferin successfully docked

withamyloid-β (Aβ) peptide. The ligand binding affinity was high due to the presence of many

flexible docked conformers with the most stable conformer having a binding energy of -12.513

kcal/mol. The analysis illustratesthe use of a computational approach to assess the use of mangiferin

as a potential nutraceutical.

Keywords: Mangiferin, β-amyloidpeptide (Aβ),Alzheimer’s disease, AutoDock, Binding Energy


Page | 26

C5 [21]

IDENTIFICATION AND In-Sillico CHARACTERIZATION OF A NOVEL Hindiii

ISOSCHIZOMER FROM A Pseudomonas SPP

S.N.J. Pathirana1, M. Kajan1, D.A.S. Elvitigala1, G.H.C.M. Hettiarachchi1,

C.M. Nanayakkara2, R.G.S.C. Rajapakse3 and N.V. Chandrasekharan1

1Department of Chemistry, University of Colombo, Colombo 03, Sri Lanka

2Department of Plant Sciences, University of Colombo, Colombo 03, Sri Lanka 3Department

of Molecular Biology and Biotechnology, Faculty of Science, University of Peradeniya,

Peradeniya, Sri Lanka

ABSTRACT

Type II Restriction enzymes (REs) can be used to cleave double stranded DNA in a sequence specific

manner to produce fragments with desired ends. Herein, we report the first attempt to screen and

isolate REs from bacteria in Sri Lankan soil and water samples collected from different regions of the

country. Bacteria isolated from samples were grown in Luria-Bertani (LB) medium. Lysates were

prepared and aliquots incubated with lambda DNA, and gel electrophoresed. Distinct banding patterns

indicate the presence of REs. Nine isolates belonging to different genera (Lysinibacillus,

Acinetobacter, Pseudomonas and Brevibacillus) as confirmed by morphological and molecular

characterization using 16S rRNA were positive for the presence of restriction endonucleases. The

lysate of one isolate belonging to the genus Pseudomonas was found to produce a banding pattern

similar to that of lambda DNA cleaved with HindIII. The enzyme was partially purified and optimum

conditions for enzyme activity and its recognition sequence was determined. The whole genome of

the Pseudomonas sp was sequenced and the gene for the putative HindIII isoschizomer identified

together with genes coding for other restriction enzymes. The gene coding for the HindIII

isoschizomer was bioinformatically analyzed and its homology and evolutionary relationship to other

known isoschozomers of HindIII determined. Molecular docking studies were also carried out using

its DNA ligand, reinforcing its functionality. Work is underway to clone the gene for production of

the recombinant enzyme.

Keywords: Restriction Enzymes, HindIII, Isoschizomer Whole genome sequencing


Page | 27

POSTER

PRESENTATIONS


Page | 28

P1 [22]

DETERMINATION OF DESIRABLE ATTRIBUTES OF A MEMBER OF

Burkholderia cepacia COMPLEX AS A BIOLOGICAL CONTROL AGENT OF

PLANT PATHOGENIC FUNGI

V.N.D. Bulathsinhalage and D.M. De Costa

Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Sri

Lanka

ABSTRACT

The present study was conducted to determine some of the desirable attributes of a bacterium

belonging to Burkholderia cepacia complex as a potential biological control agent in integrated

management of postharvest diseases of fruits and vegetables. In vitro assay was done by dual culture

plate technique using five fungal pathogens causing economically-important postharvest diseases,

namely, Botryodiplodia spp., Fusarium spp., Pestalotia spp., Colletotrichum gloeosporioides and

Colletotrichum capsici. The antagonistic ability of the bacterium and the cell-free filtrate of the

bacterium against the fungal pathogens was determined under in vitro conditions at the ambient

temperature. Moreover, antagonistic ability of the bacterium against Botryodiplodia spp. at different

temperatures (27, 37, 47, 57, 67 and 77 oC) was also determined under in vitro conditions. The

member of the B. cepacia complex showed a 58%, 45%, 59%, 50% and 54% colony growth

inhibition of Botryodiplodia spp., Fusarium spp., Pestalotia spp., C. gloeosporioides and C. capsici,

respectively at the 6th day of incubation. The antagonistic ability of the bacterium retained till 47 oC

and gave the same percentage colony inhibition against Botryodiplodia spp. However, cell-free

filtrate, stored at ambient temperature did not show antagonism against the fungal pathogens used for

the study. The findings revealed the broad-spectrum ability of the bacterium to be used for the

management of postharvest fungal pathogens and the ability of integrating the bacterium with an

appropriate hot water treatment for the management of postharvest diseases.

Keywords: Postharvest diseases, Botryodiplodia spp., Antagonism, Cell-free filtrate, Dual plate

method


Page | 29

P2 [23]

MOLECULAR CHARACTERIZATION OF GENUS Puntius USING

MITOCHONDRIAL GENE MARKERS FROM RIVERS OF SOUTHERN WESTERN

GHATS, INDIA

A.A. Arunkumar

Department of Biotechnology, Karpagam Academy of Higher Education, Tamil Nadu, India

ABSTRACT

Freshwater ecosystems contain only about 0.01% of the Earth’s water and the system accounts about

45% of fish species known to date. Alteration in the micro and macro habitats in the system severely

affects the aquatic life especially fishes and also complicates the fish taxonomy. Among the different

kinds of fresh water fishes, the fishes grouped under the genus Puntius, belonging to Cypriniformes

family, have been considered as an important protein source of daily diets of human beings in south

India, in particular. The inter-genetic and intra-genetic relationships of the Puntius species have not

yet been well understood to date and the species are reported to exist in various water bodies of

peninsular India. In the present study,the genomic DNA was isolated and PCR amplification was

carried out using universal primers for partial mitochondrial CO1 and 16sRNA sequences of

approximately 650bp length. Based on the obtained sequence of phylogenetic tree was constructed

using the Neighbour-Joining method using by Molecular Evolutionary Genetics Analysis (MEGA).

The present study provides a clear indication of the patterns of CO1sequence divergence within and

among the species.Mitochondrial CO1and 6sRNA genes resolve the taxonomic ambiguities of

genus Puntius. The less intra-specific variation was observed than the intraspecific variation in

both CO1 and 16sRNA genes and the phylogenetic tree expresses all the species of Puntius from

Southern Western Ghats to originate from a common ancestral group (i.e. all the species of the

genus Puntius has similar molecular status by which they fall under a single phylogenetic clad)

further more supporting to the field of Taxonomy of fresh water fishes of India. The significances

of the study and timely measures needed to protect the species have also been concisely discussed.

Keywords: Puntius, Southern Western Ghats, CO1, 16sRNA, Phylogeny


Page | 30

P3 [24]

DEVELOPMENT OF MICROBIAL INOCULUM TO ENHANCE THE

DEGRADATION OF ORGANIC WASTE

J.K.A. De Silva1, G.A.U. Jayasekera1 and C.M. Nanayakkara1

1Department of Plant Sciences, University of Colombo, Sri Lanka

ABSTRACT

Organic waste has become a critical issue with rising population, urbanization and economic activities

in Sri Lanka. There are number of methods that are used for organic waste management majoring

organic composting. However, the main problem of this process is that it needs much time to degrade

naturally. Therefore, this study is focused on the development of microbial consortium to enhance the

degradation of organic waste. This study found out the effective method to reduce the time taken for

degradation of 5kg of organic waste within 30 days by using Pseudomonas sp., Bacillus sp. and

Aspergillus sp. which are isolated from the Kaduwela garbage site, Sri Lanka. These microorganisms

were selected based on their extracellular enzyme activity by an enzyme assay and identified by using

biochemical and morphological identification methods. There were two types of treatments that were

used as individual and combination of the above mentioned microorganisms in three different

concentrations such as Mcfarland 1, 3 and 5 of bacterial isolates and spore suspension of Aspergillus

sp. which equals to 3x108spores /ml, 9x108spores/ml and 1.2x109spores/ml. Each of these

concentrations was applied individually and 3 combined concentrations as low (T1), moderate (T2)

and high (T3) of above mentioned microorganisms. The efficiency of degradation was analyzed by

comparing C/N ratio, mean of degradation using One-way-Analysis of Variance (ANOVA) and

degradation percentage. According to the results, the highest concentration of combined consortium

(T3), showed the lowest C/N ratio as 15:1 and the highest mean of degradation as 66.67%. With

regard to the use of One-way ANOVA for combination, the mean value of control and T1 (P = 0.004),

control and T2 (P = 0.001), control and T3 (P = 0.000) results obtained revealed that there were

significantly different, confirming that highest concentration of combination of these three

microorganisms was the best consortium to degrade organic waste efficiently.

Keywords: Organic waste, microbial consortium, gravimetric analysis, C/N ratio


Page | 31

P4 [25]

ENHANCED PRODUCTION OF Bacillus thuringiensis SUBSPECIES israelensis

DELTA ENDOTOXIN BY SUPPLEMENTATION OF ROTTEN MANGO JUICE

AND FISH-AMINO ACID AS MEDIUM SUPPLEMENTS

C. Gopinathan

Bioprocess Laboratory, Department of Biotechnology, University of Calicut, Kerala, India

ABSTRACT

Mosquito borne diseases not only cause a loss of lives but also impose heavy health and economic

burdens. Extensive use of chemical insecticides for the control of malaria and other mosquito borne

diseases has led to the development of resistance in mosquitoes to these insecticides and hazards to

the environment. Biolarvicides of the strain Bacillus thuringiensis israelensis, serotype H-14 is highly

effective against mosquito larvae. Even though Bti products are efficient controls for mosquito and

black fly larvae, their use in developing countries is limited by their cost. Thus, there is a need to

reduce the overall production cost of Bti in order to make it competitive in the market. It depends on

many factors; however, the raw material cost is one of the most important criteria, which may

comprise >70% of the overall production cost. Fruit wastes are available in plenty and contains

mainly fructose as the carbon source, which is easily frementable and can susbstitute costly

susbstrates like glucose. Channelising huge quantities of rotten/waste mangoes which other wise are

discarded can substantially reduce production cost of Bti. Similarly fish -amino acid produced by

fermenting rotten fish and jaggerry/molassess has proved to be an excellent as a medium supplement,

especially to overproduce the much wanted delta endotoxin produced by Bti. India is one the counties

leading in fruit and vegetable production.It is also blessed with one of the longest coastline in the

world of approx. 7516.6 km. The total annual catch is around 4 million metric tonnes. In addition it is

second after brazil in sugar cane cultivation with annual yield of 3412 million metric tons. The

massive availability of fruit wastes (mangoes) and huge quantities of rotten /discarded fish , which are

freely available, all can be channelised for cost effective production of this value added product,

substantially lowering the media cost of Bti production when scale- up is attempted. Results show

biomass increase of upto 29% compared to control, when rottenmangoe juice was used as the main

carbon source. The toxicity improvements with fish -amino acid supplemented medium shows

considerable reduction in killing time of Aedes aegyptii larvae.

Keywords: Bacillus thuringiensis subspecies israelensis, rotten mango, fish amino acid, delta

endotoxin, agro wastes


Page | 32

P5 [26]

RHEOLOGICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF HIGH

PRESSURE PROCESSED CITRUS JUICES

S. Karlović, T. Bosiljkov, F. Dujmić, F. Troha, M. Škegro, M. Marelja, M. Brnčić and

D. Ježek

Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

ABSTRACT

Since most bioactive compounds and some physical properties degrade during high-temperature

pasteurization, novel high pressure (HHP) food processing technology is investigated as a potentially

viable alternative. Changes in textural and rheological properties of citrus fruits (Citrus x clementina)

were analyzed in fresh juice (reference values), after conventional pasteurization, as well as after

high-pressure processing (200, 400 and 600 MPa during 4, 8 and 12 min at 25 oC). Shelf life and

antioxidant activity were also investigated. Based on obtained results and concluded statistical

analysis (ANOVA, Tukey) process parameters having p < 0.5 were chosen as adequate. The color of

pasteurized juices degraded significantly, while in HHP juices remains very close to reference values,

with E=1,65 during whole shelf life. Viscosity and density of HHP treated juices also remained

stable and within 3,2 % of fresh juices. There were no observed changes in pH values. A total

antioxidant capacity value was 369,61 mgL-1 in fresh juice, with less than 5 % loss observed at 400

MPa. Pressures over 400 MPa could be considered as excessive, and optimal process parameters were

400 MPa at 12 min. This optimized HHP process yields significantly better results than conventional

processing, and the product is in all aspects comparable to fresh juice during whole investigated shelf

life.

Research in this paper is part of Croatian Science Foundation research project HIGHPRESS-JUICE.

Keywords: high hydrostatic pressure, clementine juice, rheological properties, TOAC


Page | 33

P6 [27]

BENIGN FABRICATION OF COPPER NANOPARTICLES FROM FLOWER

EXTRACT OF Cassia auriculata L

G.P. Chandran 1, T. Priyadharshini1, C. Rajkuberan1, N. Sekar1 and D.S. Kumar2

1Dept of Biotechnology, FASH, Karpagam Academy of Higher Education, Tamil Nadu, India

2Department of Biochemistry, FASH, Karpagam Academy of Higher Education, Tamil Nadu,

India

ABSTRACT

The present study was aimed to synthesis copper nanoparticles (CuNPs) in a greener route using

aqueous flower extract of Cassia auriculata. Initially, synthesis of CuNPs was confirmed through

UV-Vis spectroscopy which shows the surface plasmonic resonance peak (SPR) at 276nm. FTIR

analysis provides clear evidence that protein fractions present in the flower extract act as reducing and

stabilizing bio-agents. Energy dispersive X-ray (EDAX) spectroscopy confirms the presence of

copper as a major constituent element. Electron microscopic studies such as Field emission scanning

electron microscopic (Fe-SEM) and Transmission electron microscope (TEM) reveals that

synthesized CuNPs are spherical in shape with the size range between 5 and 30 nm. Further, flower

aqueous extract and synthesized CuNPs were evaluated against different bacterial and fungal

pathogens and when compared to the flower extract, biosynthesized CuNPs exhibits a remarkable

antimicrobial activity. The inhibition zones were ranged from 10-15 mm. Likewise C. auriculata

synthesized CuNPs demonstrated cytotoxic activity against tested Colon cell lines (CaCo2) and Dog

kidney cell lines (MDCK). The IC- 50 value for the C. auriculata CuNPs against CaCo2 and MDCK

cell lines were found to be 40 μg/ml respectively. The output of this study clearly suggesting that

biosynthesized CuNPs using aqueous flower extract of C. auriculata can be used as promising

nanomaterial for therapeutic application in context with nano-drug formulation.

Keywords: Cassia auriculata; Copper, Antibacterial and toxicity


Page | 34

P7 [28]

BIOSYNTHESIS OF SILVER NANOPARTICLES USING LEAF EXTRACT OF

Zingiber officinale (GINGER), Allium cepa VAR. Aggregatum (SHALLOT), Capsicum

annuum (BIRD’S EYE CHILI) AND Curcuma longa (TURMERIC):

DETERMINATION AND COMPARISON OF ANTIOXIDANT, ANTIMICROBIAL

PROPERTIES WITH LEAVES WATER EXTRACTION

Y.D.B. Peramuna and M. Kandiah

BMS, School of Science, Sri Lanka

ABSTRACT

Most of the disease conditions occurs due to an imbalance between free radicals and antioxidant

capacity. Therefore, antioxidants intake by daily diet to maintain the homeostasis of free radicals and

antioxidants to minimize the risk of having disease conditions. This novel study focused the

determination of antioxidant and antimicrobial activities in four different plant leaves of Zingiber

officinale (Ginger), Allium cepa var. aggregatum (Shallot), Capsicum annuum (Bird’s eye chili) and

Curcuma longa (Turmeric) in Sri Lanka. The water extraction of each sample was used to determine

antioxidant capacity by Total Antioxidant Capacity (TAC) assay, Total Phenolic Content (TPC) assay

and Total Flavonoid Content (TFC) assay, ABTS radical scavenging activity, IC50 and ferric

reducing ability of plasma (FRAP) assay. Furthermore, leaves water extract samples were used to

determine antimicrobial activity on test organisms Escherichia coli (gram-negative) and

Staphylococcus aureus (gram-positive) by well diffusion technique. The formation of silver

nanoparticles from ginger, shallot, Bird’s eye chili and turmeric were confirmed by UV-

spectrophotometry spectrum which gave a peak absorbance values around 400 nm. The study was

further enhanced to compare antioxidant capacity and activity along with antimicrobial activity of

silver nanoparticles with leaves water extract. The statistical analysis of P < 0.05 (P = 0.031599) only

in TAC assay indicates a significant difference between leaves water extract and silver nanoparticles,

confirming highest total antioxidant capacity (741.818 g/dm3) to be present in silver nanoparticles.

The silver nanoparticles showed the highest antimicrobial activity on both Escherichia coli (2.65 cm)

and Staphylococcus aureus (2.10 cm).

Keywords: silver nanoparticles, Total Antioxidant Capacity, Total Phenolic Content, Total Flavonoid


Page | 35

P8 [29]

AN IRIDOID GLYCOSIDE FROM THE SRI LANKAN MEDICINAL PLANT

Wendlandia bicuspidata

S. Rajendran1,2, S. Mohotti2, E.D. de Silva2,U. Göransson1,S. Gunasekera1 and C.M.

Hettiarachchi2

1Division of Phamacognosy, Department of Medicinal Chemistry, Uppsala University,

Biomedical Centre, Sweden

2Department of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri

Lanka

ABSTRACT

We are presently engaged in the screening of Sri Lankan medicinal plants belonging to the family

Rubiaceae for bioactive metabolites. Wendlandia bicuspidata Wight & Arn is a large tree found in the

hilly region of Sri Lanka, mostly in Central province that has medicinal applications in traditional

medicine. The presence of Scandoside methyl ester has been reported previously fromW. bicuspidata.

Now we report the isolation and identification of Ixoside from the aqueous leaf extract. The plant was

collected in the area Uda Peradeniya in central province and it was identified by the National

herbarium, Peradeniya. Air dried, powdered leaf material was sequentially extracted first with Hexane

then dichloromethane, Ethylacetate and finally with methanol. A high yield of crude compound was

obtained from themethanolic extract, showing antibacterial activity in the agar dick diffusion assay.

Methanolic crude extract was partitioned with water to obtain water dissolvable polar compounds.

The water extract was fractionated on preparative high performance liquid chromatography with

gradient elution using ACN/H2O. Fractions obtained from HPLC were further analyzed by mass

spectrometry to obtain mass profiles of the fractions. The fraction with the compound Ixoside was

further purified using analytical HPLC and characterized with MS and MSMS techniques. The

structure of the molecule was elucidated with NMR techniques C13, H1, COSY, HMBC and HSQC.

Ixoside was obtained as a white water soluble compound with Minimum Inhibitory Concentration

against Staphylococcus aureus at 100 µg/ml.

Keywords: Wendlandia bicuspidata, Iridoid glycoside, NMR


Page | 36

P9 [30]

THE APPLICATION OF SURFACTANTS IN VOLATILE ORGANIC COMPOUND

(VOC) REMOVAL

L. Haddad, Z. Shareefdeen and H. Ahmed

American University of Sharjah, Sharjah, UAE

ABSTRACT

Volatile organic compounds (VOCs) are currently being emitted in large quantities; thus stringent

regulations are imposed on industry to control emission of toxic VOC pollutants. This works

compares the advantages and disadvantages of VOC removal alternatives which are mainly divided

into two categories, destruction and recovery. For the destruction techniques, it explores thermal

oxidation, catalytic oxidation, and bio-filtration. As for the recovery techniques, it investigates

adsorption, condensation, membrane separation, and most importantly absorption. The main focus of

the research was to look into the application of absorption as a technique to reduce the concentration

of the VOCs (i.e. toluene) through exploring different surfactant solutions as absorbents. Different

types of surfactant solutions were looked into for optimum removal efficiencies, and for the

operational and design parameters which affect them. Finally, some environmental implications were

explored which include the hazards of both VOCs and surfactants, but most importantly the reuse and

regeneration of surfactant solutions.

Keywords: volatile organic compounds, surfactants, absorption, toluene


Page | 37

P10 [31]

THE STUDY OF GENETIC VARIABILITY IN RELATED TO EFFICIENCY OF

ZINC OPTAKE IN DIFFERENT GENOTYPES OF TRITICUM AESTIVUM L.

Abdollah Hassanzadeh Ghorttapeh1, Aziz Majidi1, Javad Mozafari 2, Abdohosin Zyaeian3

1Horticulture Crops Department, West Azarbaijan Agricultural and Natural Resources

Research and Education Center, Agricultural Research, Education and Extinction

Organization, (AREEO), Urmia, Iran.

2Agricultural Research, Education and Extinction Organization, Tehran, Iran.

3Soil and Water Research Institute (SWRI). AREEO, Karaj, Iran.

ABSTRACT

Diversity in agro ecosystems can improve the sustainability of cropping systems in a context of low

external inputs and unpredictable climate change. Agricultural ecosystems are related to economical

and society condition widely, that there are in world. The major target of agricultural ecosystems

management is maximum nutrient flow and human service materials. Nutrient efficiency in wheat is

very complex. It includes nutrient acquisition efficiency and nutrient use efficiency. 26 winter wheat

genotypes were used to investigate the interactive effects between genotypes and the use efficiencies

of the Zn micronutrient by the grain. An experiment was carried out in Agricultural and Natural

Resources Research and Education, Center of West Azerbaijan, Genotypes were planted in complete

randomized block design with three replications. The traits such as plant height, biological and grain

yield, harvest index, length of spike, number of kernel per spike, thousand kernel weight, weight of

spike Zn uptake in grain were measured or calculated. It was revealed that biomass weight of whole

plot, plant height, number of kernel per spike, grain yield of main culms, biological yield, length of

spike and number of spikelet/spike, were positively and grain protein percentage, negatively correlate

with grain yield. Variance analysis showed that there were highly significant differences among traits.

The results obtained in this study indicate that nutrient use efficiency of the Zn varies widely within

wheat genotypes. Some genotypes were identified as being Zn use efficiency. These are considered

low-input genotypes. It appears that a special breeding programmer of crop cultivars for low Zn

nutrient and stress condition could be successful. Improved cultivar response to Zn nutrient will help

to reduce inputs and hence protect the environment.

Keywords: Wheat, Zn use efficiency, Genetic variability, low input

http://www.swri.ir/HomePage.aspx?lang=en-US&site=DouranPortal&tabid=1


Page | 38

VIRTUAL

PRESENTATIONS


Page | 39

[32]

STOCK IDENTIFICATION AND SOME BIOLOGICAL ASPECTS OF FRIGATE

TUNA (Auxis thazard) IN SRI LANKAN WATERS

D.R. Herath1,2, U. Seneviratne1, H.A.C.C. Perera1 and G.H.C.M. Hettiarachchi2

1Marine Biological Resources Division, National Aquatic Resources Research and

Development Agency, Colombo 15, Sri Lanka

2Department of Chemistry, Faculty of Science, University of Colombo, Colombo 3, Sri Lanka

ABSTRACT

This study was carried out to determine the biology and the genetic stock structure of Frigate tuna,

Auxis thazard, an important neritic tuna fish species found in the Indian Ocean. A total of 354 frigate

tuna samples were collected from October 2015 to September 2017 from the coastal areas of Chilaw

and Kalpitiya in the North western province, Negombo and Beruwela in the Western province,

Dodanduwa, Galle, Weligama and Mirissa in the Southern province and Trincomalee in the Eastern

province of Sri Lanka. The length weight relationship for the collected samples was shown to be

W=0.828L3.433, which shows that the species has a healthy growth pattern. The analysis of the

stomach contents showed that they are nonselective feeders, feeding on diverse prey items such as

small fish species (herrings, sardines), shrimps, cephalopods and stomatopod larvae. The fecundity of

female A. thazard was shown to be 48,056 to 267,000 eggs. The GSI for female A. thazard fish

peaked during the period May to July indicating that their spawning season is during this period. The

stock structure was analyzed by studying the sequences of the mitochondrial COI region and the

mitochondrial DNA D-loop region. The phylogenetic analysis carried out using 21 sequences of

mitochondrial COI region and 23 sequences of mitochondrial control region, showed the fish of the

different regions studied being clustered together for both molecular markers. Therefore, when

management plans are being developed for A.thazard, the stocks of the different coastal areas could

be considered as belonging to one unit.

Keywords: Frigate tuna, Auxis thazard, stock identification, feeding, fecundity


Page | 40

[33]

PRODUCTION OF PROTEIN HYDROLYSATES FROM FISH SKIN FOR DAIRY

PRODUCTS

E.I. Titov, G.V. Semenov, I.I. Ionova, I.S. Krasnova and N.S. Kozlov

Department “Technology and Biotechnology Meat, Fish and Dairy Products”, Moscow State

University of Food Production, Russia

ABSTRACT

The aim of this research was study the fish protein hydrolysates (FPH) from Salmon (Salmo salar)

skin prepared by different methods. The four methods: enzymatic hydrolysis, acid hydrolysis,

chemical (with acids or alkali) and enzymatic hydrolysis with preserving agents upon the heating

proposed to produce fish protein hydrolysates (FPH). All methods modified and adapted to optimize

researches. All hydrolysates were vacuum freeze-dried and collagen content was determined in them.

The results showed that FPH prepared using enzymatic hydrolysis with preserving agents upon the

heating had greater amount of protein than FPH prepared using other methods. The initial fish odour

and off-flavours generated in fish hydrolysate were masked by chitosan solution, citric acid

preparation and sesame seeds. The sensory analysis of the finish product allowed choosing the most

rational content of components. This FPH may potentially serve as a good source of protein. It could

be as an emulsifier and as a foaming agent in dairy products.

Keywords: fish protein hydrolysates, hydrolysis, fish odour, citric acid preparation, sesame seeds,

chitosan


Page | 41

[34]

INFLUENCE THE PARAMETERS OF FREEZE DRYING ON ANTIOXIDANT

ACTIVITY OF YOGHURTS WITH DIFFERENT TROPICAL FRUITS

G.V. Semenov, I.I. Ionova, Z.V. Volokitina and I.S. Krasnova

Department “Technology and Biotechnology Meat, Fish and Dairy Products”, Moscow State

University of Food Production, Russia

ABSTRACT

Main patterns of interrelationship between process parameters and the preservation of dry

components in final product were determined in this article. Tropical fruits (jackfruit, melon, and

banana) were frozen in pieces. The process of freeze drying of the fruit raw material was at the

temperature of phase converting ice-to-vapour (from 100C) below zero to 350C below zero) at an

appropriate vacuum level. At the secondary drying the temperature was always the same, equal to 38-

40 °C. The final moisture content of the dried samples was 1.7-2.0%. Freeze drying fruits were easily

breakable and converted into powder. Each sample of dry fruit’s powder was added to the yogurts

during the fermentation. The total antioxidant activity was measured in the finished products by

coulometric titration with electrogenerated bromine using an EKSPERT 006 coulometric analyzer.

Researches showed that freeze drying at increasingly lower primary drying temperatures leads to an

increase the content of antioxidants.

Keywords: vacuum freeze drying, tropical fruits, yogurt, antioxidant activity, coulometric titration


Page | 42

[35]

INFLUENCE OF HIGH HYDROSTATIC PRESSURE ON PHYSICAL

PROPERTIES OF APPLE JUICE

T. Bosiljkov, K. Klas, S. Karlović, F. Dujmić, M. Škegro, M. Marelja, M. Brnčić and

D. Ježek


ABSTRACT

Innovative and eco – friendly technologies are widely used in food processing with minimum

modification on the sensorial and organoleptic characteristics. The main goal of this research was an

analysis of the influence of the high hydrostatic pressure on physical properties of apple juice. The

influence of high hydrostatic pressure on absorbance, density, and particle size distribution was

observed under following conditions: Pressure (p = 200, 400, 600 MPa); Time of treatment (t = 5, 10,

20, 30 minutes). The treatment was conducted on apple juice obtained with filter diameter 0,3 and 0,5

mm. Statistically significant influence of process parameters is expressed over p – values. ANOVA

factors having p<0,05 were taken into consideration. The stability of apple juice can be observed by

measuring the absorbance and expressed by JSI [h] (Juice Stability Index). Apple juice made with

filter diameter 0,5 mm has greater stability where values of the JSI are in the range 47 – 224 h with

optimal process parameters by 400 MPa and 20 minutes (232 h); 600 MPa and 10 minutes (244 h).

Particle size distribution depending on relative frequency indicates stability in the obtained interval

between 0,1 – 3 µm. High hydrostatic pressure and time of treatment have no significant influence on

density value.

Keywords: high hydrostatic pressure, apple juice, juice stability index, particle size distribution


Page | 43

[36]

DEGRADATION OF 2,4,6 TNT BY NATIVE MICROORGANISMS ISOLATED

FROM TNT CONTAMINATED SOIL

M. Devasena and A. Thenmozhi

Sri Krishna College of Technology, Coimbatore, Tamilnadu, India

ABSTRACT

Soil contamination from toxic explosive is a major environmental concern at military shooting ranges,

storage sites and disposal areas in many parts of the world.2,4,6-Trinitrotoluene (TNT) is the most

widely used secondary explosive material and it constitutes a special contamination problem.TNT and

its derivatives are toxic and recalcitrant and are biodegraded relatively slowly in the environment. The

present work aims to isolate, characterize and enrich the microbial population in TNT contaminated

samples collected from different sites. The feasibility of using Bacillus subtilis, Pseudomonas

aeruginosa, Staphylococcus aureus and Serratia marcescens for TNT degradation was analysed

under various experimental conditions. Change in soil properties and change in TNT concentration

were made to understand the tolerance limit of the native microorganisms. Biodegradable ability of

the bacterial population was then performed with different concentrations of TNT. The degradation

potential, metabolites and the pathway for degradation were then elucidated and confirmed by HPLC

analysis. The native microbes were able to degrade TNT and may be possessing a huge potential for

enhanced degradation of contaminants.

Keywords: TNT, aerobic, anaerobic, Bacillus, subsurface


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[37]

MULTI FEATURES CLUSTERING APPROACH TO DETERMINE

PARKINSONIAN DISEASE UPON GAIT MOVEMENT ARTIFACTS

S. Anujan and M. S. Karunarathne

Sabaragamuwa University of Sri Lanka

ABSTRACT

Parkinson’s disease is a neurodegenerative brain disorder that progresses slowly in most elderly

people. Symptoms can take years to develop, and most people live for many years with the disease.

The symptoms caused by Parkinson’s include an ongoing loss of motor control as well as a wide

range of non-motor symptoms. Since the victims of Parkinsonian disease are senior citizens who often

have difficulties in motor ability, it is challenging to identify the symptoms of Parkinsonian disease at

the initial stages. Therefore, we have attempted to distinguish eight Parkinson’s patients from eight

healthy subjects using their gait information. Our analysis is based on cumulative vertical ground

reaction forces collected from 16 Sensors located under the left foot and right foot of 16 Parkinsonian

and non-Parkinsonian subjects during usual walking. We have applied time varied entropy techniques

(Sample Entropy and Approximate Entropy) and fast Fourier transform over the sensor measurements

sampled at 100 Hz. We calculated the mean values of sample entropy and approximate entropy

relevant to gait movement for each left leg and right leg of each subject. The fourth dominant

frequency were calculated for each gait movements conducting fast Fourier transform over sensor

measurements. The calculated Sample Entropy, Approximate Entropy and fourth dominant frequency

were ranged respectively (-4.4 to -5.2), (0.02 - 0.06) and (10 Hz- 65 Hz). The three features (Sample

Entropy, Approximate Entropy and Fourthly dominant frequency) of the movements were plotted in

three dimensional sphere. Indeed, the Parkinsonian patients and healthy subjects could be grouped

handily in to two clusters with 100% accuracy.

Keywords: Parkinson’s disease, Fast Fourier Transform, Sample Entropy, Approximate Entropy, Gait

Analysis


Page | 45

[38]

ULTRASONIC - ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM

WASTE IN PRODUCTION OF RED WINE

F. Dujmić, S. Karlović, T. Bosiljkov, F. Troha, M. Škegro, M. Marelja, D. Ježek, R. Vidrih

and M. Brnčić


ABSTRACT

Grapes as the fruit are produced more than 60 million tons per year and are processed into wine and

other grape products, and consequently are source of large quantities of organic waste. Wine lees are

one of the by-products of the wine production. Wine lees mainly refer to remaining sediment in

vessels containing wine after fermentation and during the maturing of the wine. grape skins and seeds

contain large quantities of bioactive compounds, such as anthocyanins. These compounds can be

extracted using conventional techniques such as maceration, or novel nonthermal extraction

technologies that include high hydrostatic pressure, high-intensity ultrasound, as well as pulsed

electric fields. In this research, are investigated influence of ultrasonic parameters - on yield of

extracted polyphenolic compounds from wine lees of Blatina cultivar. Effect of process parameters

such as extraction time (five different times from 60 s to 2700 s), amplitude (five different values

from 20 % to 100 %) of 400 W ultrasonic processor and probe diameter (7 mm, and 40 mm) at

starting extraction temperature at 25 °C on quantity and composition of extracted bioactive

compounds was investigated. Optimization of ultrasound-assisted extraction of anthocyanins from

Blatina cultivar wine lees is carried out using Design Expert software. Results show increases

extraction yield from 20 % to 70 %. Optimal process time of ultrasonic extraction was up to 80 times

shorter compared to conventional extraction. Calculation of optimal UAE process parameters based

on the obtained data shows that the best results occur using 90 % of maximal amplitude, during 1800

s using 40 mm ultrasonic probe.

Keywords: Ultrasound, wine lees, extraction parameters, HPLC, antioxidants


Page | 46

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