Book of Abstracts2012/12/04 · Erika Wahyu Dewanti, Kunihiko Futami, Takayuki Katagiri, Makoto...

JSPS-NRCT Asian Core University Program Symposium 2012

TOKYO

Development of new bio-technology for

aquaculture and risk management of aquaculture

products-III

Rakusui Hall, Shinagawa Campus

Tokyo University of Marine Science and Technology

Supported by Japan Society for the Promotion of Science (JSPS), Tokyo University of Marine Science and Technology,

and National Research Council Thailand (NRCT)

場所：東京海洋大学品川キャンパス楽水会館　日程：平成24年12月7日（金）　

7 December, 2012

National Research Council Thailand

Book of Abstracts

JSPS-NRCT Asian Core Program Symposium 2012

“Development of new bio-technology for aquaculture and risk management of aquaculture products-III”

7 December, 2012

Shinagawa Campus, Rakusui Hall,

Tokyo University of Marine Science and Technology, Tokyo, Japan

9:00-9:30 Registration

9:30-9:45 Opening ceremony

Nobuaki Okamoto, President of TUMSAT

Nontawith Areechon, Coordinator of Asian Core Program, Kasetsart University

Brief Introduction of the program

Ikuo Hirono, Coordinator of Asian Core Program, TUMSAT

9:45-10:15 KEYNOTE LECTURE

Varin Tanasomwang (Coastal Fisheries Research and Development Bureau, Department of Fisheries,

Thailand)

CURRENT STATUS OF COASTAL AQUACULTURE IN THAILAND

INVITED PAPERS

10:30-10:50

Mudjekeewis D. Santos (National Fisheries Research and Development Institute, Philippines)

FISHERIES AND AQUACULTURE BIOTECHNOLOGY IN THE PHILIPPINES

10:50-11:10

Han-Ching Wang1*

, Chu-Fang Lo2, Yun-Tzu Huang

1, Yi-An Chiang

1, Hsin-Yi Hung

1 (1Institute of

Biotechnology, National Cheng Kung University, Taiwan, 2Institute of Zoology, National Taiwan

University, Taiwan)

WSSV PATHOGENESIS AND SHRIMP INNATE IMMUNITY WITH SPECIFICITY

11:10-11:30

Alimuddin1*

, Irmawati1, Aras Syazili

1, Muhammad Safir

1, Anna Octavera

1 and Goro Yoshizaki

2

(1Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University,

Indonesia, 2Tokyo University of Marine Science and Technology, Japan)

ADMINISTRATION OF RECOMBINANT FISH GROWTH HORMONE TO IMPROVE

GROWTH OF GIANT GOURAMI JUVENILE

11:30-11:50

Mai Nam Hung (College of Aquaculture and Fishery, Can Tho University, Vietnam, Tokyo University of

Marine Science and Technology, Japan)

THE SITUATION OF AQUACULTURE IN VIETNAM

11:50-12:10

Mutaqin Ahmad1 and Masengi Simson (Directorate Fish Processing, Ministry of Marine Affairs and

Fisheries, Republic of Indonesia)

TRACEABILITY SYSTEM OF SHRIMP PRODUCTS FROM FARM TO TABLE

(INDONESIA)

12:10-12:30

Yusma Yennie1*

, Ratih Dewanti-Hariyadi2 and Achmad Poernomo

1 (

1Research Center for Marine and

Fisheries Product Processing and Biotechnology, Agency for Marine and Fisheries Research and

Development, Ministry of Marine and Fisheries, 2Department of Food Science and Technology, Faculty of

Agricultural Engineering and Technology, Bogor Agricultural University, Indonesia)

PREVALENCE OF PATHOGENIC VIBRIO PARAHAEMOLYTICUS ISOLATED FROM

SHRIMP CULTURE IN WEST JAVA, INDONESIA

12:30-13:45 LUNCH & POSTER SESSION

13:45-14:00

Chettupol Polchan1, Wanlapha Molex

1, Supaporn Hnuchu

1, Suwit Wuthisuthimethavee

1*, Nubuaki

Okamoto2, Takashi Sakamoto

2 Sataporn Direkbusarakom

1, and Piyapong Chotipun

1 (1Shrimp Research

Unit, School of Agricultural Technology, Walailak University, THAILAND, 2Faculty of Marine Science,

Tokyo University of Marine Science and Technology, JAPAN) SNPS RELATED TO GROWTH IN THE BLACK TIGER SHRIMP (PENAEUS MONODON)

14:00-14:15

Muhammad Fadhil Syukri Ismail, Toyoko Nakajima, Masamichi Nakajima (Laboratory of Population

Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, Japan)

EFFECT OF INBREEDING GUPPY POECILIA RETICULATA TOWARD HEAT

RESISTANCE AND GENETIC VARIATIONS

14:15-14:30

Kozue Yamashita, Hidehiro Kondo and Ikuo Hirono (Tokyo University of Marine Science and

Technology, Japan)

DEVELOPMENT OF DNA VACCINES AGAINST PSEUDOTUBERCULOSIS IN JAPANESE

FLOUNDER, PARALICHTHYS OLIVACEUS

14:30-14:45

Thawonsuwan Jumroensri1, Kasornchandra Jiraporn

2*, Soonsun Pacharee

3, Pomwised Rattanaruji

4

Keawtapee Chantana5 (1Coastal Aquatic Animal Health Research Institute, Coastal Aquaculture and

Development Bureau, Department of Fisheries, Thailand, 2Department of Fisheries, Thailand,

3Krabi

Coastal Fisheries Research and Development center, Coastal Aquaculture and Development Bureau,

Department of Fisheries, Thailand, 4Department of Microbiology, Faculty of Science, Prince of Songkla

University, Thailand, 5Phuket Coastal Fisheries Research and Development center, Coastal Aquaculture

and Development Bureau, Department of Fisheries, Thailand)

EFFICACY OF AN INACTIVATED VACCINE AGAINST VIRAL NERVOUS NECROSIS

(VNN) IN SEA BASS (LATES CALCARIFER)

14:45-15:00

Piti Amparyup1,2

, Walaiporn Charoensapsri1, and Anchalee Tassanakajon

2* (1Center of Excellence for

Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science,

Chulalongkorn University, THAILAND, 2National Center for Genetic Engineering and Biotechnology

(BIOTEC), National Science and Technology Development Agency (NSTDA), 12120, THAILAND)

MOLECULAR MECHANISMS REGULATING THE PROPHENOLOXIDASE ACTIVATING

SYSTEM IN THE SHRIMP Penaeus monodon

15:00-15:15

Tidaporn Chaweepack1*

, Chutima Khomvilai2, Surachart Chaweepack

1, Lila Ruangpan

1, Kaeko Kamei

3

(1Chanthaburi Coastal Fisheries Research and Development Center, Department of Fisheries, Thailand,

2Marine Shrimp Research Institute, Department of Fisheries, Thailand,

3Department of Biomolecular

Engineering, Kyoto Institute of Technology, Japan)

EFFECT OF GALANGAL (ALPINIA GALANGA LINN.) EXTRACT ON GROWTH,

SURVIVAL RATE, VIBRIO HARVEYI AND WHITE SPOT DISEASES RESISTANCE IN

PACIFIC WHITE SHRIMP, LITOPENAEUS VANNAMEI

15:15-15:30 Hiroki Koyama

1*, Sanit Piyapattanakorn

2 and Shugo Watabe

1,3† (

1Laboratory of Marine Biochemistry,

Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan, 2Departmant of

Marine Science, Faculty of Science, Chulalongkorn University, Thailand, 3Kitasato University School of

Marine Bioscience, Japan, *Present address: Kitasato University School of Marine Bioscience, Japan)

NOVEL SKELETAL MYOSIN HEAVY CHAIN ISOFORMS FROM ADULT PLEOPOD

MUSCLE OF COMMERCIALLY IMPORTANT SHRIMPS

15:30-16:15 COFFEE BREAK & POSTER SESSION

16:15-16:30

Sasimanas Unajak1*

, Napat Songtawee1, Orathai Sawadechaikul

1, Anchalee Tassanakajon

2, Nontawith

Areechon3, and Kiattawee Choowongkomon

1,4 (

1Department of Biochemistry, Faculty of Science,

Kasetsart University, THAILAND, 2Department of Biochemistry, Faculty of Science, Chulalongkorn

University, THAILAND, 3Department of Aquaculture, Faculty of Fisheries, Kasetsart University,

THAILAND, 4Center for Advanced Studies in Tropical Natural Resources, National Research

University-Kasetsart University, Thailand)

TWO STRATEGIES TO IDENTIFY THE YELLOW HEAD VIRUS (YHV) PROTEASE

INHIBITORS: HOMOLOGY MODELING AND VIRTUAL SCREENING OF PROTEASE

AND RNA APTAMER

16:30-16:45

Chettupol Polchan1, Khwanta Phoonsamran

1, Suwit Wuthisuthimethavee

1*, Ikuo Hirono

2, Hidehiro

Kondo2, Sataporn Direkbusarakom

1 and Piyapong Chotipuntu

1 (

1Shrimp Research Unit, School of

Agricultural Technology, Walailak University, THAILAND, 2Faculty of Marine Science, Tokyo

University of Marine Science and Technology, JAPAN)

IDENTIFICATION OF VITELLOGENIN GENE IN POLYCHAETE

16:45-17:00

Ubonrat Limtipsuntorn1*

, Yutaka Haga1, Hidehiro Kondo

1, Ikuo Hirono

1, Shuichi Satoh

1 (Tokyo

University of Marine Science and Technology, Japan)

EFFECTS OF DIETARY FISH AND VEGETABLE OILS ON THE HEPATIC

TRANSCRIPTOME IN JAPANESE FLOUNDER JUVENILES PARALICHTHYS OLIVACEUS

17:00-17:15

Haruhiko Toyohara1*

, Wen Liu1, Xuegang Li

1, Yoshiyuki Araki

1, Wituspong Salikput

2, Koji Shiomi

2

(1Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan,

2FIC-LACTO CO., LTD, Bangkok 10230, Thailand)

PROBIOTIC SHRIMP CULTURE IN JAPAN AND THAILAND – UTILIZATION OF

ENZYMES FROM TRADITIONAL FERMENTED FOODS

17:15-17:30

Erika Wahyu Dewanti, Kunihiko Futami, Takayuki Katagiri, Makoto Endo and Masashi Maita (Tokyo


PRACTICAL STUDIES ON INDONESIAN HERB FOR PREVENTING CHEMICAL

CONTAMINATION

17:15-17:30

Nguyen Thu Hong1*

, Masaaki Kodama1, Gen Kaneko

2, Yasuwo Fukuyo

1 (1Asian Natural Environmental

Science Center, The University of Tokyo, Japan, 2Department of Marine Biochemistry, The University of

Tokyo, Japan)

PARALYTIC SHELLFISH POISONING (PSP) TOXIN – BINDING SUBSTANCE IN

BACTERIA PTB-1

17:30-17:45

Wannisa Boonsong1,2

, Wanwimol Klaypradit1,2

and Pongtep Wilaipun1,2*

(1Faculty of Fisheries, Kasetsart

University, THAILAND. 2Center for Advanced Studies for Agriculture and Food, Institute of Advanced

Studies, Kasetsart University, THAILAND)

ANTIOXIDANT ACTIVITY OF SELECTED EDIBLE MUSHROOM EXTRACTS

OBTAINED FROM DIFFERENT EXTRACTANTS

17:45-18:00

Pongtep WILAIPUN1, Takeshi ZENDO

2, Jiro NAKAYAMA

2 and Kenji SONOMOTO

2 (

1Faculty of

Fisheries, Kasetsart University, Thailand, 2Faculty of Agriculture, Kyushu University, Japan.)

NOVEL BACTERIOCINS PRODUCED BY BACTERIAL STRAINS ISOLATED FROM THAI

FERMENTED FISH PRODUCTS

18:30~ Banquet

Poster session

1. Noratat Prachom, Shuichi Satoh, Yutaka Haga (Tokyo University of Marine Science and Technology,

Japan)

BIOVAILABILITY OF NUTRIENTS AND AMINO ACIDS IN CORN PROTEIN

CONCENTRATE WITH RAINBOW TROUT (ONCORHYNCHUS MYKISS)

2. Feng Lu, Shuichi Satoh, Yutaka Haga (Tokyo University of Marine Science and Technology, Japan)

REPLACEMENT OF FISH MEAL WITH RENDERED ANIMAL PROTEIN AND PLANT

PROTEIN SOURCES ON GROWTH AND BIOLOGICAL PERFORMANCE OF RAINBOW

TROUT

3. Wen Liu1, Shingo Maegawa

2, Haruhiko Toyohara

1* (1Division of Applied Biosciences, Graduate

School of Agriculture, Kyoto University, Japan, 2Division of Intelligence Science and Technology,

Graduate School of Informatics, Kyoto University, Japan)

DETERMINATION OF THE “WETLAND SEDIMENTS ACTING AS BIOREACTORS”

HYPOTHESIS FROM THE STANDPOINT OF THE MECHANISM OF CELLULOSE

BINDING DOMAIN (CBD) OF A CELLULASE OF A BRACKISH CLAM CORBICULA

JAPONICA

4. Y .Q. Huang1, S. Satob, M. Higuchi

2, T. Hirai

3 and M. Nakajima

1 (

1Department of Marine Life

Science and Population Genetic Informatics, Graduate School of Agriculture Science, Tohoku University,

Japan, 2Niigata Prefectural Inland Water Fisheries Experiment Station, Japan,

3Department of Life &

Health Science, Teikyo University of Science, Japan)

DEVELOPMENT OF THE SEX-LINKED DNA MARKER IN COMMON CARP (CYPRINUS

CARPIO)

5. Takafumi Mikami1*

, Masato Sato2 and Masamichi Nakajima

1 (

1Graduate School of Agriculture Science,

Tohoku University, Japan, 2Ani branch, Akita Prefetural Fisheries Center, Japan)

GENETIC EFFECT OF RELEASED MASU SALMON (O. MASOU) ON WILD

POPULATIONS

6. Mochammad Riyanto1 and Takafumi Arimoto

2* (

1Graduate Course of Applied Marine Biosciences,

Tokyo University of Marine Science and Technology, TJapan, 2Department of Marine Biosciences,

Tokyo University of Marine Science and Technology, Japan)

EXERCISE EFFECT ON SWIMMING PERFORMANCE OF JACK MACKEREL

TRACHURUS JAPONICUS, EXAMINED BY MUSCLE TWITCH EXPERIMENT

7. Aiko Shitara1, Takashi Koyama

1, Kana Goto

1, Yuya Shigenobu

2, Takuma Sugaya

2, Motohiko Sano

2,

Hidehiro Kondo1, Ikuo Hirono

1 (

1Laboratory of Genome Science, Tokyo University of Marine Science

and Technology, Japan, 2Aquatic Genomics Research Center, National Research Institute of Fisheries

Science, Japan)

IDENTIFICATION OF WSSV HOMOLOGUES IN THE GENOMIC DNA OF KURUMA

SHRIMP, MARSUPENAEUS JAPONICUS

8. Apichaya Taechavasonyoo, Ikuo Hirono, Hidehiro Kondo (Laboratory of Genome Science, Tokyo


THE IMMUNO-ADJUVANT EFFECT OF INTERLEUKIN-1! IN JAPANESE FLOUNDER

(PARALICHTHYS OLIVACEUS)

9. Sheryll Hipolito, Hidehiro Kondo, Ikuo Hirono (Laboratory of Genome Science, Tokyo University of


ROLE OF MARSUPENAEUS JAPONICUS CRUSTIN-LIKE PEPTIDE AGAINST VIBRIO

PENAEICIDA AND WHITE SPOT VIRUS INFECTION

10. Ryo Inoue, Hidehiro Kondo, Ikuo Hirono (Laboratory of Genome Science, Tokyo University of


NON-SPECIFIC IMMUNE ACTIVATION AGAINST DOUBLE STRANDED RNA IN

KURUMA SHRIMP, MARSUPENAEUS JAPONICUS

11. S. Kanazawa, G. Kato, H. Kondo, and I. Hirono (Laboratory of Genome Science, Tokyo University

of Marine Science and Technology, Japan)

CHANGE IN IMMUNE GENE EXPRESSION PATTERN OF KOI HERPESVIRUS

INFECTED COMMON CARP, CYPRINUS CARPIO

12. Kana Gotoh, Hidehiro Kondo, Ikuo Hirono (Laboratory of Genome Science, Tokyo University of


EXHUAUSTIVE ANALYSIS OF SPECIFIC GENE EXPRESSING IN HEMOCYTE FOR

MARSUPENAEUS JAPONICAS BY MICROARRAY

13. Nichika Sakai, Ikuo Hirono, Hidehiro Kondo (Laboratory of Genome Science, Tokyo University of


TEMPERATURE-DEPENDED REGULATION OF IMMUNE-RELATED GENE

EXPRESSION IN FISH CULTURE CELLS

14. Satomitsu Suda1, Ikuo Hirono

1, Hidehiro Kondo

1, Takashi Aoki

2 (

1Laboratory of Genome Science,

Tokyo University of Marine Science and Technology, Japan, 2Consolidated Research Institute for

Advanced Science and Medical Care, Waseda University, Japan)

SEQUENCING OF VARIABLE REGION OF IMMUNOGLOBULIN NEW ANTIGEN

RECEPTOR GENES IN THE BANDED HOUNDSHARK, TRIAKIS SCYLLIUM

15. Shoichiro Aoka, Hidehiro Kondo, Ikuo Hirono (Laboratory of Genome Science, Tokyo University of


EFFECT OF CODON OPTIMIZED DNA VACCINE AGAINST NOCARDIA SERIOLAE TO

ANTIGENIC EXPRESSION IN JAPANESE FLOUNDER, PARALICHTHYS OLIVACEUS

16. The Han Nguyen and Toshiaki Ohshima (Department of Food Science and Technology, Graduate

School of Marine Science and Technology, Tokyo University of Marine Science and Technology)

THE NATURAL ANTIOXIDANT ERGOTHIONEINE: RESOURCES, CHEMICAL

CHARACTERIZATION, AND APPLICATIONS

17. Mintra Seel-audom1, Nontawith Areechon

2, Kunihiko Futami

1*, Takayuki Katagiri1, Makoto Endo

1

and Masashi Maita1 (

1Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan,

2Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand)

THE EXPRESSION OF BIOTRANSFORMATION PATHWAY GENES OF NILE TILAPIA

(OREOCHROMIS NILOTICUS) INDUCED BY LEUCOMALACHITE GREEN ON DIETARY

EXPOSURE

18. Mari Inada1, Keisuke Kihara

2, Ayaka Shiki

2, Tomoya Kono

3, Terutoyo Yoshida

4, Masahiro Sakai

4,

Tohru Mekata5, Raja Sudhakaran

6 and Toshiaki Itami

4,* (

1Interdisciplinary Graduate School of

Agriculture and Engineering, University of Miyazaki, Japan, 2Graduate School of Agriculture, University

of Miyazaki, Japan, 3Interdisciplinary Research Organization, University of Miyazaki, Japan,

4Faculty of

Agriculture, University of Miyazaki, Japan, 5National Research Institute of Aquaculture, Oita, Japan,

6School of Bio-Sciences and Technology, VIT University, India)

ASTAKINE, AN ANCIENT CYTOKINE, IN KURUMA SHRIMP MARSUPENAEUS

JAPONICUS

19. Mari Inada1, Keisuke Kihara

2, Ayaka Shiki

2, Tomoya Kono

3, Terutoyo Yoshida

4, Masahiro Sakai

4,

Tohru Mekata5, Raja Sudhakaran

6 and Toshiaki Itami

4,* (




4Faculty of

Agriculture, University of Miyazaki, Japan, 5National Research Institute of Aquaculture, Oita, Japan,

6School of Bio-Sciences and Technology, VIT University, India)

FREE RADICAL GENERATION ENZYMES IN KURUMA SHRIMP MARSUPENAEUS

JAPONICUS

20. Keisuke Kihara2, Mari Inada

1, Ayaka Shiki

2, Tomoya Kono

3, Terutoyo Yoshida

4, Masahiro Sakai

4,

and Toshiaki Itami4,*

(1Interdisciplinary Graduate School of Agriculture and Engineering, University of

Miyazaki, Japan, 2Graduate School of Agriculture, University of Miyazaki, Japan,

3Interdisciplinary

Research Organization, University of Miyazaki, Japan, 4Faculty of Agriculture, University of Miyazaki,

Japan)

CHARACTERIZATION OF TOLL INTERACTING PROTEIN GENE, MJTOLLIP, IN

KURUMA SHRIMP (MARSUPENAEUS JAPONICUS)

21. Toshiaki Itami1,*

, Mari Inada2, Junich Nishi

3, Maki Yoshimine

3, Keisuke Kihara

3, Ayaka Shiki

3,

Tomoya Kono4, Terutoyo Yoshida

1, Masahiro Sakai

1, Tohru Mekata

5, Raja Sudhakaran

6 and Shogo

Okugawa7 (

1Faculty of Agriculture, University of Miyazaki, Japan,




5National

Research Institute of Aquaculture, Oita, Japan, 6School of Bio-Sciences and Technology, VIT University,

India, 7Science Center of Tensei Suisan, Saga, Japan)

EXPRESSION ANALYSIS OF IMMUNO-RELATED GENES DURING WSSV INFECTION

IN KURUMA SHRIMP, MARSUPENAEUS JAPONICUS

22. Takashi Aoki1,2

, Jun-ichi Hikima2, Ho-Bin Jang

2, Tae-Sung Jung

2, Janenuj Wangtavatchai

3,

Haruko Takeyama4, Hidehiro Kondo

5 and Ikuo Hirono

5 (

1Consolidated Research Institute for

Advanced Science and Medical Care, Waseda University, Tokyo, Japan, 2Aquatic

Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National

University, Jinju, Gyeongnam, South Korea, 3Department of Veterinary Medicine, Faculty of

Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 4Department of Life Science

and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku Tokyo 162-8480,

Japan, 5Laboratory of Genome Science, Tokyo University of Marine Science and Technology,

Tokyo, Japan)

A NEW TRANSFERABLE MULTIDRUG-RESISTANCE PLASMIDS DERIVING FROM

HUMAN ENTERIC OR ENVIRONMENTAL MICROORGANISMS: DISCOVERING FROM

AEROMONAS HYDROPHILA IN TILAPIA FARM, THAILAND

23. Sanit Piyapattanakorn and Parinthon Veerapattananon (Chulalongkorn U)

PRELIMINARY STUDY ON GENETIC DIVERSITY OF IRRAWADDY DOLPHIN,

ORCAELLA BREVIROSTRIS, IN SONGKHLA LAKE, THAILAND

ABSTRACTS

JSPS-NRCT Asian Core Program Symposium 2012 Tokyo

7 December 2012,

Tokyo University of Marine Science and Technology,

Tokyo, Japan !

! "!

!

!

!

!

"#$$%&'!(')'#(!*+!,*)(')-!).#),#-'#$%!/&!01)/-)&2!

#$%&'!($'$)*+,$'-!

.*$)/$0!1&)23%&3)!43)3$%52!$'6!73830*9+3'/!:;%3$;*>!"?@??

(2$&0$'6D!!(2$&0$'6!9%*83)!&/)30=!/*!G3!$!5*;'/%F!*=!2&-2!9*/3'/&$0!$'6!);553))!&'!5*$)/$0!$E;$5;0/;%3D!!H'!

%353'/!F3$%)

9%*6;5/&8&/F! G;/! $0)*! &'! /3%+)!*=! E;$0&/F! $'6! )$=3/F!*=! 9%*6;5/)! /2%*;-2*;/! /23!9%*6;5/&*'! 0&'3D! ! (23!

/*/$0! $E;$5;0/;%3!9%*6;5/&*'!*=! (2$&0$'6! &'!I?"?!,$)!"

$E;$5;0/;%3!&'!I?"?!,$)!SKI


7 December 2012,


Tokyo, Japan !

! "!

!"#$%&"%#'()*'+,-(.-/0-&%'1"20%.$)2/234'")'0$%'5$"/"66")%#'

!

!

7-*8%9%%:"#';'%3!#?#!@1'7%,!=+&/)(/!A;%B3!C>%D1&!

E('2!##??!F7(5(--(&%6B!

!

GE1,,%6-1&9(&+!/>'71,H!9I(%6/&'16J+!

!

!!!!! K7%!F7(5(--(&%6!(6!/!L(67(&+!&/'(1&!/&9!(6!1&%!1L!'7%!0(++%6'!L(67%,(%6!(&!'7%!M1,59!/'!&>,%! -,19>)'(1&! /&9! R

,9! (&! 6%/M%%9!

-,19>)'(1&B! ='! (6! )1&6(9%,%9! /6! 1&%! 1L! '7%! L%M! :(6'/&'! S/'%,! *(67(&+! 4/'(1&6! T:S*46U! +510/552B!

:16'/(&/0(5('2! 1L! )1>&',2W6! L(67%,(%6! ,%61>,)%6!

/&9!9(;%,6('2!(6!'7,%/'%&%9!02!>&6>6'/(&/05%!L(67(&+!-,/)'()%6!/&9!&1M!)5(,%!

-,19>)'(1&!(6!/LL%)'%9!02!>&6>6'/(&/05%!/Q>/)>5'>,%!-,/)'()%6!/&9!9(6%/6%6B!X%,%3!M%!-,%6%&'!1>,!,%6>5'6!

1&!>6(&+!+%&%'()63!-/,'()>5/,52!:4A3!'1!+%&%,/'%! (&L1,%6!

6>,,1>&9(&+!)15/'(1&!6',>)'>,%6!1L!-%5/+()!L(67!6-%)(%6!6>)7!/6!'>&/63!6/,9(&%63!&9!(&!F7(5(--(&%!M/'%,63! (&!Y1>'7%/6'!A6(/!/&9!(&!'7%!S%6'%,&!/&9!E%&',/5!F/)(L()!/6! (&->'6!'1!6'1)N!

/66%665'>,%93! (&9(+%&1>6! /&9!

%&9/&+%,%9!/Q>/'()! 6-%)(%6! '1! )5/,(L2! '/Z1&1&)%,'/(&'(%6! /&9! L1,! 6'1)N! %&7/&)%--,%66(1&! Y>0',/)'(;%! X20,(9(D/'(1&! T4$Y\YYXU!

)(9/'%!+%&%!%Z-,%66(1&!1L!'(+%,!67,(,;(;%9!SYY]!(&L%)'(1&!/&9!>6%!'7(6!'1!/)>5'>,%3!F7(5(--(&%63!)1


7 December 2012,


Tokyo, Japan !

! "!

!

"##$!%&'()*+,+-.-!&,/!#(0.12!3,,&'+!3114,.'5!6.'(!#2+7.8.7.'5!!

!9&,:;(.,*!"&,*

<!=>!;(4:?&,*!@)

A>!B4,:CD4!94&,*!

<>!B.:E,!;(.&,*

<>!9-.,:B.!94,*!

<!!

!#$%&'(')'*!+,!-(+'*./%+0+123!45'(+%50!6/*%1!7)%1!8%(9*:&('23!;5(%5%3!5%!!

?$%&'(')'*!+,!@++0+123!45'(+%50!;5(>5%!8%(9*:&('23!;5(A*(3!#=B3!;5(>5%!!

!

C6+::*&A+%D(%1!5)'/+:E!>5%1/.FG5(0H%.I)H*D)H'>!

!

J(%.*!#KK=3!'/*!&/:(GA!(%D)&':2!/5&!&),,*:*D!,:+G!.+%&(D*:5L0*!0+&&*&!D)*!'+!9(:50!5%D!L5.'*:(50!

D(&*5&*!A:+L0*G&3!(%.0)D(%1!>/('*!&A+'!D(&*5&*!MNJOP3!>/(./!(&!.5)&*D!L2!>/('*!&A+'!&2%D:+G*!9(:)&!

MNJJQPH!$%!'/(&!A:*&*%'5'(+%3!$!>(00!(%':+D).*!'/*!'*./%+0+1(.50!A05',+:G&H!N*!/59*!)&*D!'+!5DD:*&&!

.)::*%'!./500*%1(%1!(&&)*&!(%!/+&'!(GG)%('2!5%D!NJJQ!A5'/+1*%*&(&H!R(:&'3!$!>(00!+)'0(%*!/+>!>*!/59*!

)&*D!&2&'*G&!L(+0+12!'+!&')D2!'/*!10+L50!(%'*:5.'(+%&!L*'>**%!NJJQ!5%D!('&!/+&'H!;/(&!5AA:+5./!/5&!

500+>*D!)&!'+!.+%&':).'!5!10+L50!.*00!A5'/>52!%*'>+:I!'/5'!&/*D&!%*>!0(1/'!+%!'/*!A)SS0*!+,!9(:)&T/+&'!

(%'*:5.'(+%&H!-2!)&(%1!1*0T,:**!A:+'*+G(.&3!G*'5L+0+G(.&!5%D!0(A(D+G(.&3!>*!,+)%D!'/5'!NJJQ!(%D).*&!

D(&'(%.'!.*00)05:!:*&A+%&*&!5'!D(,,*:*%'!:*A0(.5'(+%!&'51*&U!&A*.(,(.50023!'/*!N5:L):1!*,,*.'!(%!'/*!NJJQ!

1*%+G*!:*A0(.5'(+%!&'51*3!5%D!.*00!D*5'/!(%!'/*!05'*!&'51*H!NJJQ!(&!'/*!,(:&'!(%9*:'*L:5'*!9(:)&!*9*:!'+!L*!

:*A+:'*D!'+!(%D).*!5!N5:L):1T0(I*!*,,*.'!(%!('&!/+&'V&!.*00&H!NJJQ!(&!50&+!'/*!,(:&'!%+%T.5%.*:!(%D).(%1!

9(:)&!'+!(%D).*!5!N5:L):1T0(I*!*,,*.'H!W+:*+9*:3!NJJQT(%D).*D!N5:L):1!*,,*.'!(&!':(11*:*D!L2!/+&'!

G;XY!A5'/>52H!$%!'/*!&*.+%D!A5:'!+,!'/(&!A:*&*%'5'(+%3!$!>(00!D*&.:(L*!/+>!O&.5G!MO+>%!&2%D:+G*!

5D/*&(+%!G+0*.)0*P3!5!G*GL*:!+,!'/*!(GG)%+10+L)0(%!&)A*:,5G(023!A052&!5!:+0*!(%!&/:(GA!(%%5'*!

(GG)%('2!>('/!&A*.(,(.('2H!N*!*950)5'*D!'/*!:*05'(+%&/(A!L*'>**%!&/:(GA!O&.5G!5%D!&/:(GA!(GG)%('23!

5%D!./5:5.'*:(S*D!'/*!:*&A+%&*&!5%D!*ZA:*&&*D!95:(5L0*!(&+,+:G&!+,!O&.5G!515(%&'!95:(+)&!A5'/+1*%(.!

(%95&(+%&H!N*!50&+!D(&.)&&*D!/+>!'/*!O&.5G!(&+,+:G!&A*.(,(.('2!.+G*&!5L+)'H!N*!L*0(*9*!'/*!>+:I!

A:*&*%'*D!/*:*!(&!9*:2!(GA+:'5%'!L+'/!'+!&.(*%.*!5%D!'+!'/*!5[)5.)0'):*!(%D)&':2H!

!

F+56)0/-G!&2&'*G&!L(+0+123!NJJQ3!N5:L):1!*,,*.'3!O&.5G3!(%%5'*!(GG)%('2!>('/!&A*.(,(.('2!

!


7 December 2012,


Tokyo, Japan !

! "!

!"#$%$&'()'$*%+*,+(-.*#/$%)%'+,$&0+1(*2'0+0*(#*%-+'*+$#3(*4-+1(*2'0+*,+1$)%'+1*5()#$+

654-%$7-++

!7$#5""$%89:+;(#)2)'$

8:+!()&+$7$

8:+?50)##)"+


7 December 2012,


Tokyo, Japan !

! "!

!

"#$!%&"'("&)*!)+!(,'(-'."'/$!&*!0&$"*(1!

123!*24!#567!

#$%%&'&!$(!)*+,-+%.+/&!,01!2345&/67!#,0!85$!903:&/43.67!;3&.0,

8$=6$!903:&/43.6!$(!>,/30-&!?-3&0-&!,01!8&-50$%$'67!@,A,0!

!

B3.5!$:&/!CDEE!=

4./3A&!-,.(345!OP,0',43+4!56A$A5.5,%


7 December 2012,


Tokyo, Japan !

! "!

!"#$%#&'(')*+,*-)%.+/0+,1"'.2+3"/45$)-+0"/.+6#".+)/+!#&(%+7894/9%-'#:+

;5)#

)'A9*&+&4! -:-9'0-%*0!;4! %A9*&)%01!(*:0)&%'-!+&'!-)%

)*! J'&%5%(+)%*0! +()%J%)4! ).'! -4-)'A! %-! 4')! '55'()%J'! )*! 9&'J'0)! 7')'0)%*0! *5! -.&%A9! 9&*7:()-! 5&*A!

%A9*&)%01!(*:0)&%'-!;'(+:-'!).'!%A9


7 December 2012,


Tokyo, Japan !

! "!

!"#$%'(#)*+)!%,-*.#'/()!"#$"%&'($()(*+%,-."/01)01*&%,#2)+"*3)4-"/35)67&,7"#))

/')8#1,)9%$%:)0'2*'#1/%)

;713%);#''/#%,/-)?#@%',/AB%"/C%2/

D)%'2)E(-3%2)!*#"'*3*

<)

#$%&%'()*!+%,-%(!./(!0'(1,%!',2!31&*%(1%&!4(/25)-!4(/)%&&1,6!',2!71/-%)*,/8/69:!

!;6%,)9!./(!0'(1,%!',2!31&*%(1%&!$%&%'()*!',2!! ?%,-:!01,1&-(9!/.!0'(1,%!',2!31&*%(1%&!!@'(-?%,-!/.!3//2!A)1%,)%!',2!B%)*,/8/69:!3')58-9!/.!;6(1)58-5('8!C,61,%%(1,6!',2!B%)*,/8/69:!!

!7/6/(!;6(1)58-5('8!!!!!D,1=%(&1-9!!

!

E+/((%&>/,21,6!'5-*/(F!9%,,1G95&?'H9'*//I)/?!!

! J,!J,2/,%&1':!>(%&%,)%!/.!>'-*/6%,1)!!"#$"%&'($()(*+%,-."/01!1,!&%'.//2!*'&!)'5&%2!&%=%('8!

%K>/(-!(%L%)-1/,I!!B*1&!(%&%'()*!'1?%2!-/!12%,-1.9!>(%&%,)%!/.!>'-*/6%,1)!!"#$"%&'($()(*+%,-."/01!.(/?!

-('21-1/,'8!',2!1,-%,&1=%!&*(1?>!>/,2&!1,!J,2('?'95:!M%&-!N'='I!B*1&!&-529!&*/O%2!-*'-!#PQR@STUVW!',2!

PQR@!S#XI"TVW!&'?>8%&!.(/?!-('21-1/,'8!',2!1,-%,&1=%!>/,2&!)/,-'1,%2!!"#$"%&'($()(*+%,-."/01:!

(%&>%)-1=%89I!C16*-9!/,%!>%()%,-!S#RQ#PW!/.!!"#$"%&'($()(*+%,-."/01!!1&/8'-%2!!.(/?!-('21-1/,'8!>/,2&!

',2!TUV!SRQPW!/.!-*/&%!/Y-'1,%2!.(/?!1,-%,&1=%!>/,2&!O%(%!>'-*/6%,1)!Y'&%!-/!-*%1(!>/&&%&1/,!/.!.2)!

6%,%I!!M*%,!6%,!%,)/21,6!./(!.$)!O'&!5&%2!'&!-*%!Y'&1&!./(!)8'&&1.1)'-1/,:!#TQ#P!SZ[VW!',2![QP!S!P"VW!/.!

!"#$"%&'($()(*+%,-."/01!/Y-'1,%2!!.(/?!-('21-1/,'8!',2!!1,-%,&1=%!!>/,2&:!(%&>%)-1=%89:!O%(%!>'-*/6%,1)I!!

\5-!/.!-*%!@@!!"#$"%&'($()(*+%,-."/01!!1&/8'-%&:!#P!S"@I"VW!O%(%!>'-*/6%,1)!Y'&%2!/,!-*%!>/&&%&1/,!/.!

6%,%!%,)/21,6!./(!.2)&',2!#Z!SXPI[VW!)',!Y%!)8'&&1.1%2!'&!>'-*/6%,!Y'&%2!/,!-*%!!.$)!6%,%I!!\=%('88:!

>'-*/6%,1)!!"#$"%&'($()(*+%,-."/01!O'&!./5,2!!'-!'!!.(%]5%,)9!/.!#R^#TQR@!S[RVW!/.!-*%!&*(1?>!&'?>8%&!

.(/?!-('21-1/,'8!',2!R^[QR@!S##VW!/.!&*(1?>&!.(/?!1,-%,&1=%!>/,2&!(%&>%)-1=%89I!!!!

!

!

F#C@*"21F!!"#$"%&'($()(*+%,-."/01:!&*(1?>!)58-5(%:!.2)!',2!.$)!6%,%&!


7 December 2012,


Tokyo, Japan !

! "!

!"!#$!"%&'()&*")+",%+-)."/#").&"0'(12")/,&%"!.%/3$"4!"#$"%&'()#)*)#5"

#$%&&'()*!+)*,$-./0!1-.*-($-!2)*%3

/0!4'(-()5.!6.',$'

/0!4'78&!1'&$89'&$8:%&$-;%%

/

='>'-?8!@?-:)&)A0!B-?-9$8!4-?-:)&)

A!4-&-()5.!C85%?>'9-5-?):

/0!-.D!+8E-().F!#$)&8('.

/!

!/4$58:(!G%9%-5,$!H.8&0!4,$))*!)I!JF58,'*&'5-*!B%,$.)*)FE0!1-*-8*-?!H.8;%598&E0!=-?$).!48!B$-::-5-&!"K/L/0!B6JMNJ=C!

AC%(-5&:%.&!)I!2-58.%!O8)9,8%.,%90!P-,'*&E!)I!2-58.%!4,8%.,%0!B)?E)!H.8;%598&E!)I!2-58.%!4,8%.,%!-.D!B%,$.)*)FE0!B)?E)!!

!!/K"Q"RSS0!TJ+J="

"

)($)58.!M0!:)*&!8.$8>8&8.F!$)5:).%Q/0!:)*&!8.$8>8&8.F!$)5:).%QA!-.D!9%,5%&%D!(5)&%8.!-.D!

-,8D8,!-.D!58,$!8.!,E9&%8.%!F%.%9!7%5%!98F.8I8,-.&*E!-99),8-&%D!).!$8F$!F5)7&$!9$58:(!I-:8*8%9!X+YKWK/ZW!

@I!&$)9%!()*E:)5($89:90!4=+9!7%5%!I)'.D!&)!>%!-99),8-&%D!78&$!$8F$!F5)7&$!8.!&$%!>*-,?!&8F%5!9$58:(!

-.D!&$%!4=+!:-5?%59!7%5%!&$%.!D%;%*)(%D!&)!'9%!;8-!6G2!&%,$.8['%!8.!&$%!9%*%,&8).!)I!$8F$!F5)7&$!

>*-,?!&8F%5!9$58:(!>5))D9&),?9W!!

26789:;


7 December 2012,


Tokyo, Japan !

! "!

!""#$%&'"&()*+##,-).&/0112&!"#$%&%'()#*%$+&'*'&3'45+,&6#5%&7#8-8%5)$#&5),&/#)#%-$&95+-5%-')8&

!:0;5;3!01&(39!/0137!L3(3!=31')*-39!.701=!)L3/A3!604('7%)3//0)3!

6%(?3(7F!51&(33901=!4'3++04031)!N+P8!3M-34)39!%19!'&73(A39!;3)3('U*='70)*!L3(3!4%/4./%)39F!V++34)7!'+!

01&(33901=!L3(3!3M%60139!01!+0A3!T.%1)0)%)0A3!)(%0)7W!(3-('9.4)0A3!(%)38!&'9*!/31=);!%)!&0();8!7.(A0A%/!%)!

9%*!#SR8!&'9*!L30=;)!%)!9%*!#SR!%19!&'9*!/31=);!%)!9%*!#SRF!2.--037!L3(3!=0A31!;3%)!7;'4?!)(3%)631)!

%)!XY'H!%19!);3!7.(A0A%/!)0637!L3(3!(34'(939F!!

H.((31)!(37./)!7.==37)39!);%)!A%(0%)0'1!7)%()39!)'!'44.(!%)!);3!QS!=313(%)0'1!'+!01&(39!6%/3!=.--*!

7;'L01=!70=10+04%1)/*!934(3%739!01!63%1!&'9*!70U37!N63%1Z:[P!+('6!#\F#ZSF#66!)'!#XFSZRF"66F!];0/38!

+36%/3!7;'L7!014(3%739!01!63%1!&'9*!70U37!+('6!#\FXZSFS66!01!);3!Q#!=313(%)0'1!)'!#\FCZSF#66!01!

);3!QS!=313(%)0'1F!:.(A0A%/!(%)3!'+!Q#!=313(%)0'1!+'(!6%/3!%19!+36%/3!07!%)!C"F"^!%19!"_FY^8!(37-34)0A3/*F!

51!);3!QS!=313(%)0'18!&');!7.(A0A%/!(%)37!934(3%739!01!6%/3!%)!CCFD^!%19!+36%/3!%)!"\FX^F!`3%)!7;'4?!

3M-3(0631)!%)!);3!Q#!=313(%)0'17!01904%)39!);%)!6%/3!=.--*!;%A3!;0=;3(!;3%)!)'/3(%143!4'6-%(39!)'!

+36%/3!%)!SRSFXZY"F\!601.)37!%19!#CRF\ZDYF\D!601.)378!(37-34)0A3/*F!51!QS!=313(%)0'178!);3!7.(A0A%/!

)063!+'(!+36%/3!=.--*!934(3%739!%A3(%=01=!%)!#_SZ#_F\X!601.)37F!$3A3/7!'+!'&73(A39!;3)3('U*='70)*!

N`'P!L3(3!/'L3(!);%1!3M-34)39!;3)3('U*='70)*!N`3P!01!QR!=313(%)0'1!N`'aRFSS8!`3aRFD#P!%19!01!Q#!

=313(%)0'1!N`'aRF\8!`3aRFD\PF!>;07!6%*!0//.7)(%)37!);3!90(34)!3++34)!'+!01&(33901=!)'L%(97!=.--*!=('L);!

93A3/'-631)F!Q.();3(!01A37)0=%)0'17!%(3!(3T.0(39!)'!%--(3;319!);3!-;*70'/'=04%/!3++34)!)'!01&(39!+07;37F!

>;07!7).9*!L0//!-('A093!+.();3(!.193(7)%1901=!01!);3!+03/9!'+!01&(33901=F!!

&

F#24'+,8G&2.--*,(!"#$%&%'()#*%$+&'*',(51&(33901=,(2313)04!b%(0%)0'178!`3%)!c3707)%143&


7 December 2012,


Tokyo, Japan !

! "#!

DEVELOPMENT OF DNA VACCINES AGAINST PSEUDOTUBERCULOSIS IN JAPANESE

FLOUNDER, PARALICHTHYS OLIVACEUS

Kozue Yamashita, Hidehiro Kondo and Ikuo Hirono

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7,

Minato, 108-8477, Tokyo, Japan.

Pseudotuberculosis or Pasteurellosis, an epizootic bacterial septicaemia has been known to occur in a

variety of fish species including Japanese flounder (Paralichthys olivaceus). An cytozoic Gram-negative

bacteriun, Photobacterium damselae subsp. piscicida (Pdp), isolated from the internal organs of diseased

fish has been identified as the causative agent of Pseudotuberculosis. Vaccines like DNA vaccines manly

induce humoral immunity and can protect against cytozoic bacterium infection. It has been reported that

antigenic genes whose codons were optimized with host codon usage could result to higher protein

sexpression. In this study, we focused on codon usage technique to develop DNA vaccines against

Pseudotuberculosis in Japanese flounder. Three antigenic genes identified by immunoscreenig. PPA1

encoding ORF of Lipoprotein, PPA2 encoding ORF of DegQ serine protease and PPARS1 encoding ORF

of outer membrane protein (wild-type) and genes that are modified based on codon usage of Japanese

flounder designated as (optimized-type). DNA vaccines were developed using three wild-type genes

(wild-ppa1, wild-ppa2 and wild-ppars1) and three optimized-type genes (opt-ppa1, opt-ppa2 and opt-

ppars1). The protective efficacy of DNA vaccines were tested in different fish groups by injecting live

Pdp (1.0!105 CFU/ml). Fourteen days post-infection with live Pdp resulted in 100% mortality in PBS

injected group. On the other hand, mortalities of group injected with wild-ppa1, wild-ppa2 and wild-

ppars1 were 16.7%, 8.3% and 58.3% while mortalities of groups injected with opt-ppa1, opt-ppa2 and

opt-ppars1 were 8.3%, 16.7% and 15.4% respectively. Those tested DNA vaccines also elicited Pdp-

specific antibody titers. In addition, the results showed upregulation of some immune genes in groups

injected with DNA vaccines compared with PBS injected group after challenge. These results indicated

that the three antigenic genes tested could be candidates for the development of effective DNA vaccine.


7 December 2012,


Tokyo, Japan !

! ""!

!"#$%!&!'(%!)$*)&*!"#$"%&'()*"+",(-*!+),(%#"*&-).*"!+#(+#"*.(-!$#*&!+/*($"*+/-!.0*

!$*%/(!,($"**!

!#$%&'()*&%(!+*,-'.()-/

"0!1%)'-(2$%(3-%!+/-%4'-(

560!7''()*(!8%2$%-..

90!8',&/).3!:%;;%(%-*$%(;%(%?!

!">'%);%@!!AB*%;/2!A(/,%@!C.%@;$!:.).%-2$!D();/;*;.0!>'%);%@!AB*%2*@;*-.!%(3!E.F.@'4,.(;!G*-.%*0!E.4%-;,.(;!'H!I/)$.-/.)0!

JK"KK0!#$%/@%(3!5E.4%-;,.(;!'H!I/)$.-/.)0!G%(LM'M!"KJKK0!#$%/@%(3!!

91-%N/!>'%);%@!I/)$.-/.)!:.).%-2$!%(3!E.F.@'4,.(;!2.(;.-0!>'%);%@!AB*%2*@;*-.!%(3!E.F.@'4,.(;!G*-.%*0!E.4%-;,.(;!'H!

I/)$.-/.)0!JK"KK0!#$%/@%(3!!=E.4%-;,.(;!'H!O/2-'N/'@'LP0!I%2*@;P!'H!72/.(2.0!8-/(2.!'H!7'(LM@%!Q(/F.-)/;P0!7'(LM$@%!JK""50!#$%/@%(3!!

?8$*M.;!>'%);%@!I/)$.-/.)!:.).%-2$!%(3!E.F.@'4,.(;!2.(;.-0!>'%);%@!AB*%2*@;*-.!%(3!E.F.@'4,.(;!G*-.%*0!E.4%-;,.(;!'H!

I/)$.-/.)0!R9""K0!#$%/@%(3!!

!

6>'--.)4'(3/(L!%*;$'-S!M)'-('(2.-(/(L!;'!;$.!-.3*2;/'(!'H!%(;/N/';/2!*).!/(!H/)$!2*@;*-.0!

;$.!F%22/(%;/'(!/)!%(!%@;.-(%;/F.!&%P!;'!2'(;-'@!;$.!.2'(',/2!@'))!2%*).3!NP!!"#$"%&'()*"+",(-U!#$.!H*;*-.!

-.).%-2$!&/@@!N.!H'2*).3!'(!/3.(;/H/2%;/'(!;$.!).-';P4.!'H!!"#$"%&'()*"+",(-!/(!'-3.-!;'!3.F.@'4!;$.!

F%22/(.U!!!!!

123456789*!"#$"%&'()*"+",(-.&G/';P4.0![-'*4.-!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%'()!$%*'+,-!-)($.'/,+.)/*$)0("0*$+"#"1,2'-$)'%/,3'/,+.)-4-/$!),+)

/*$)-*(,!0)!"#$"%&'()#)*)#)

))))

$%&%!'()*+,-)".#.!/*0*%)1+2!34*+1526*)6+%

".!*27!'284*055!9*66*2*:*;12

#<!

!"352&5+!1=!>?85005285!=1+!@1058-0*+!A%101B,!*27!C521(%86!1=!D4+%().!E5)*+&(52&!1=!A%1845(%6&+,.!F*8-0&,!1=!D8%5285.!

34-0*012B:1+2!G2%H5+6%&,.!A*2B:1:.!"IJJI.!9K'LM'NE!#N*&%12*0!352&5+!=1+!C525&%8!>2B%255+%2B!*27!A%1&5842101B,!OALP9>3Q.!N*&%12*0!D8%5285!*27!95842101B,!E5H501)(52&!'B528,!

OND9E'Q.!""J!$*410,1&4%2!R1*7.!S012B".!S012B!M-*2B.!$*&4-(&4*2%.!"#"#I.!9K'LM'NE)

)


7 December 2012,


Tokyo, Japan !

! "#!

!""!#$%&"%'()(*'()%+!"#$%$&'(&"&%(&%),--./%!0$1(#$%&*%'1&2$34%561787()%1($!4%)$*+$,'

-&+./0$%(*9%238$!%5:&$%985!(5!5%1!585$(*#!%8*%:(#8"8#%238$!%5318;:4%1$2,#/%&/34'

.&%%&5/$%

!

$%&'()*+!,-'.//('01"!23!,-45%6'!7-)68%9'%

:3!;4*'0-'*5!,-'.//('01

"3!('+

"3!7'/1)!7'6/%

#!!

!",-'+5-'?4*%!,)'@5'9!A%@-/*%/@!=/@/'*0-!'+&!B/8/9)(6/+5!,/+5/*3!B/('*56/+5!)C!A%@-/*%/@3!,-'+5-'?4*%!::DDD3!$-'%9'+&!

:E'*%+/!;-*%6(!=/@/'*0-!F+@5%545/3!Department of Fisheries3!G'+>1)1!"DHDD3!$-'%9'+&!!

#B/('*56/+5!)C!G%)6)9/049'*!I+>%+//*%+>3!7J)5)!F+@5%545/!)C!$/0-+)9)>J3!E'5@4>'@'1%3!;'1J)K143!7J)5)!LDLKMNMN3!O'('+P!!

!

2,)**/@()+&%+>!'45-)*Q!())R/.:DD:SJ'-))P0)6!!

!

F+8/@5%>'5%)+!)C!5-/!/CC/05!)C!>'9'+>'9!T!"#$%$&'(&"&%(&!*)4(U3!

*/@(/05%8/9JP!I'0-!'6)4+5!)C!>'9'+>'9!/V5*'05!.'@!6%V/&!.%5-!0)66/*0%'9!(/99/5!'+&!C/&!5)!5-/!@-*%6(!

.%5-!%+%5%'9!'8/*'>/!./%>-5!)C!5.99 ± 0.29 >!C)*!:!6)+5-@P!$-/!*/@495@!@-)./&!./%>-53!;(/0%C%0!\*).5-!

='5/!T;\=U!'+&!@4*8%8'9!*'5/!)C!5*/'56/+5!'+&!0)+5*)9!>*)4(@!./*/!+)+K!@%>+%C%0'+59J!&%CC/*/+5!T6]DPDNUP!

,9/'*'+0/!'?%9%5J!&/C%+/&!'@!(/*0/+5'>/!%+-%?%5%)+!TYFU!)C!)7'-&+./0$!'5!"!'+&!:!6)+5-!./*/!@%>+%C%0'+59J!

&%CC/*/+0/!T6^DPDNUP!$-/!+46?/*!)C!)7'-&+./0$'%+!@-*%6(!-/6)9J6(-!)C!5*/'56/+5!>*)4(!.'@!9)./*!5-'+!

%+!0)+5*)9!>*)4(P!ICC%0'0J!)C!>'9'+>'9!0*4&/!/V5*'05!/+-'+0/&!!&%@/'@/@!*/@%@5'+0/!)C!Y'0%C%0!.-%5/!@-*%6(!

'>'%+@5!)7'-&+./0$!'+&!W;;X!./*/!@-)./&!5-'5!5-/*/!./*/!@%>+%C%0'+59J!-%>-/*!@4*8%8'9!*'5/!%+!>'9'+>'9!

&%/5!C/&!>*)4(@!5-'+!5-/!0)+5*)9!>*)4(!T6^DPDNUP!A*)6!5-/!*/@495@3!./!0'+!4@/!5-%@!>'9'+>'9!0*4&/!/V5*'05!

C)*!5*/'56/+5!8%?*%)!?'05/*%'9!'+&!W;;X!&%@/'@/@P!_95/*+'5%8/!)C!4@%+>!'+5%?%)5%0!'+&!@)98/!5-/!(*)?9/6!

)C!'+5%?%)5%0!*/@%&4'9P!$-/!@-*%6(!(*)&405!%@!@'C/5J!C)*!'99!0)+@46/*@P!

%

?@ABCD%\'9'+>'9! /V5*'053% Y'0%C%0!W-%5/! ;-*%6(3' 1$2,#/%&/34' .&%%&5/$3!)$*+$,' -&+./0$3!W-%5/! @()5!

@J+&*)6/!8%*4@!TW;;XU!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&'($%$)*%&+,"'-.&/$*#,&0/*-.&-'"1"2+'&12"+&*34%)&5%$"5"3&+4'0%$&"1&0"++$20-*%%,&-+5"2)*.)&'/2-+5'&

$%&'(%!)'*+,+"-.!/+0%1!2%*+3+11+0+('&0

4!+05!/678'!9+1+:;

".!!+:5',%0+D!,7BAD;!'>!16;!+:'H;I,;01%'0;5!16&;;!B6&%,3BV!9;!&;0+,;5!16;B;!+B!!346!

+05!!347.!&;B3;A1%H;D*.!57;!1'!16;!3&;B;0A;!'>!H+&%'7B!%B'>'&,B!0;SD*!%5;01%>%;5V!^EO$I5%+36'&+B;!

B1+%0%08.!,()$,8%!6*:&%5%_+1%'0!+05!0'&16;&0!:D'1!+0+D*B%B!%05%A+1;5!16+1!16;!1%BB7;!5%B1&%:71%'0!'>!!346!

+05!!347!1&+0BA&%31B!S+B!5%>>;&;01)+D16'786!:'16!?\$B!;0A'5;5!S;&;!'>!>+B1!1*3;V!!346!1&+0BA&%31B!

S;&;!D'A+D%_;5!+1!>D;`'&!,7BAD;.!S6;&;+B!!347!1&+0BA&%31B!+1!;`1;0B'&!+05!>D;`'&!,7BAD;BV!9;!+DB'!

AD'0;5!!34B!+05!5;1;&,%0;5!16;%&!3+&1%+D!?\$!B;X7;0A;B!>'&!:D+A(!1%8;&!+05!2+A%>%A!S6%1;!B6&%,3B!+1!

_';+.!,*B%B!+05!3'B1ID+&H+D!B1+8;BV!

!!Y0!16;!3&;B;01!B175*.!S;!5;1;&,%0;5!16;!!0'H;D!!34B!>&',!+57D1!3D;'3'5!,7BAD;V!FS'!

1*3;B!'>!!34!S;&;!AD'0;5!>&',!(7&7,+!B6&%,3!+05!0+,;5!+B!!349!+05!!34:V!F6;!+,3D%>%;5!3&'57A1B!

'>!!349!+05!!34:!A'0B%B1;5!'>!bMK!:3!+05!"RKK!:3.!&;B3;A1%H;D*V!Z0D*!!349!S+B!AD'0;5!>&',!:D+A(!

1%8;&!B6&%,3V!F6;!+,3D%>%;5!3&'57A1!S+B!bMK!:3!%0!B%_;V!EB!%0!16;!A+B;!'>!(7&7,+!B6&%,3.!1S'!1*3;B!'>!

!34!S;&;!AD'0;5!>&',!2+A%>%A!S6%1;!B6&%,3!3D;'3'5!,7BAD;T!'0;!S+B!!34:!+05!16;!'16;&!!34;!0;SD*!

%5;01%>%;5V!F6;!+,3D%>%;5!3&'57A1B!'>!!34:!+05!!34;!S;&;!A',3'B;5!'>!"RK#!:3!+05!""#L!:3.!

&;B3;A1%H;D*V!F6;!36*D'8;0;1%A!+0+D*B%B!5;,'0B1&+1;5!16+1!?\$"!>&',!+:5',%0+D!,7BAD;!+05!?\$

?\$#!>&',!3D;'3'5!,7BAD;!>'&,;5!'0;!AD+5;.!%05%A+1%08!16+1!?\$%A!S6%1;!B6&%,3!3D;'3'5!,7BAD;!S+B!A'0B%5;&;5!1'!:;!'>!BD'S!1*3;!B%0A;!?\$L!S+B!

,'0'36*D;1%A!S%16!E,;&%A+0!D':B1;&!4+/"#%$)"/'#,-"(%$!/4!BD'S!1'0%A!?\$V!

6$,7"23'8!@D+A(!1%8;&!B6&%,3.!)7&7,+!B6&%,3.!!"#$%&'("'%$)*"&+(,-%$.!?*'B%0!6;+H*!A6+%0!8;0;B.!

2+A%>%A!S6%1;!B6&%,3.).'("'%$)/+(+0+(.).'("'%$)2"(("/',.!26*D'8;0*.!2D;'3'5!,7BAD;!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%!&'!()*(%$!#$*+(,!*-.$!/($.(00#"$/('+$1*&2%$3./14$5!('%($*,/*6*!#&%7$$

/#8#0#).$8#+(0*,)$',+$1*&!2'0$%9&((,*,)$#-$5!('%($',+$&,'$'5!'8(&$

$$$$

$%&'(%)%&!*)%+%,"-.-!/%0%1!$2)31%455

"-!67%18%'!$%4%95:8%',;<

"-!=):8%%&&%)%,%+2)

?-!!

/2)1%4'18!=755:82)-!%)9!!@'%11%455!A82242)3,2(2)"-!B!

!"C50%71(5)1!2D!E'2:85('&17F-!G%:;M=NO=/C!

PC50%71(5)1!2D!=Q;%:;

SA=$>/=K-!/K*R@*-!>M=NO=/CT$

$

.A2775&02)9')3!%;1827U!D&:'&&()V,;W%:W18$

$

X58575D275-! 185! 95H51%735159!2)!0215)1!H'7%85! &17;:1;75! 4%&! H%%0072]'(%15


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%!&!'(%!)$*)&*+!%#,,)-#$!$*-#$#*!$*.),/'0(#%#*

$%&''()*+!,*+-%./"0!1%2./'.!,%**/3.45./

"0!6(27'!8('%73('%74&'%.9&&

":!0!!;0!!

=7?&%75*!1*/?*>!0!!6.'.)*5/!@75&

*

:!$*55&3)*/?7/C!.('%*5T!23(27'U2(M.-M'%!

! 6&V(&/-7/C!*F!4.'(5&?!)*+B-%.&'&!W!"#$%"#"$&!3)MX!4DIG3!AB!)B5*3&V(&/-7/C!4&'%*?!5&9&.+&?!

vitellogenin as reproductive-related gene which reported in other organisms including

shrimp, the major egg yolk protein synthesized in female shrimp during gonad maturation.

Gene characterization was performed using 5’RACE and 3’RACE for full-length analysis.

Expression patterns will be examined using real-time RT-PCR during stages of maturation

and phylogenetic will be analyzed by comparing this full-length cDNA, shrimp sequences

and other invertebrates.

*

12345678T!Y7'&++*C&/7/0!D&)5*?(-'79&0!,*+B-%.&'&!W!"#$%"#"$&!3)MX!


7 December 2012,


Tokyo, Japan !

! "#!

!""#$%&'("')*#%+,-'"*&.'+/)'0#1#%+23#'(*3&'(/'%.#'.#4+%*$'%,+/&$,*4%(5#'*/'6+4+/#'"3(7/)#,'

870#/*3#&'!"#"$%&'(')*+,$%-"&./*'

'

92(/,+%':*5%*4&7/%(,/;<='>7%+?+'@+1+

;='@*)#.*,('A(/)(

;='B?7('@*,(/(

;='C.7*$.*'C+%(.

;'

!

"$%&'(&)*!+,-../!.0!1&%23*!+,2*3,*!&3'!4*,-3./.567!4.86.!932:*%;2)6!.0!1&%23*!+,2*3,*!&3'!4*,-3./.567!?.%%*;=.3'235!&()-.%@(A.3%&)BCD6&-..E,.F!

!

G2;-!.2/;!HGIJ!&%*!&!F&23!;.(%,*!.0!3KL!-25-/6!(3;&)(%&)*'!0&))6!&,2'!HM9GNJ7!O-2,-!-&;!:2)&/!

0(3,)2.3;!23!F*)&A./2,!=&)-O&6;!&3'!=-6;2./.52,&/!=%.,*;;*;E!P3!,.3)%&;)7!:*5*)&A/*!.2/;!HQIJ!&%*!

&A(3'&3)!23!3KR!&3'!3KS!=./6(3;&)(%&)*'!0&))6!&,2';!HT9GNJ!A()!'*=/*)*'!23!3KL!T9GNE!+(==/*F*3)&)2.3!

.0!QI!&;!;235/*!/2=2'!;.(%,*!0.%!F&%23*!02;-!,.(/'!23'(,*!'200*%*3)!5*3*!*U=%*;;2.3!=&))*%3;!23!/2:*%E!

4-*%*0.%*7!)-2;!;)('6!23:*;)25&)*'!*00*,)!.0!'200*%*3)!0&))6!&,2'!.3!5*3*!*U=%*;;2.3!=%.02/*!23!/2:*%!.0!

V(:*32/*;!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%#&%'()*"%+,('-.&-"/(%0(12,20(203(4*2%.203(5(6&%.%72&%#0(#8(907:+/)(8"#+(

4"23%&%#02.(;/"+/0&/3(;##3)(

(

(?@(A/0(B%-

>@(C-/D20D(B%@(E#)*%:-=%(F"2=%

>@(A%&-),#0D(G2.%=,-&

H@(I#J%(G*%#+%(

H(

!

"$%&%'%()!(*!+,,-%./!0%('1%.)1.'2!345/657.!819((-!(*!+:4%16-764.2!;

1()/%7%()!(*!79.!'./%S.)7!(*!'94%S,!,()/J!H(!&5-%/57.!79.!.**.17!(*!Q517.4%5-!.)V%S,4(&.S.)7!(*!79.!'94%S,!,()/J!H96'2!T.!.R5S%)./!79.!5SS54K./-

517%&%7%.'!T%79!&54%(6'!S(-.16-54!T.%:97'Z![L??!K$52!">?!K$52!"L?!K$5!5)/!#?!K$5J!

!H9.'.!*%)/%):!/.S()'7457./!Q517.4%5!T%79!9%:9!5S


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&$#'()%*+&,-(./(0/+./,-/(1,"2(3."(!",4,/%&/5(67,8&$#'(6./%#8&/#%&./(

9"&:#(;#7#/%&?(@(A*/&7&:.(B*%#8&

?@(C#:#


7 December 2012,


Tokyo, Japan !

! "#!

!"#"$%&'()*+,$$-'*+)./'*/0'01)2!3!4)&/5'0)6)7'08'01)*97*&"0(,)'0)7"(&,#'")!:;19%,0):+9)?/01=@A)B"*""C')D/8"E"

=A)F,0)D"0,C/

GA)H"*9I/)J9C9%/

=)

=$%&'(!)'*+,'-!.(/&,0(12(*'-!34&2(42!52(*2,6!782!9(&/2,%&*:!0;!700&%0(&(D! JI3IK! &%! '! (2+,0*0L&(! %:(C,012! M8&48! 'C/2,%2-:! ';;24*%! 8+1'(!

82'-*8!'(C!40'%*'-! %82--;&%8! &(C+%*,&2%!M0,-C!M&C2-:H! N*! &%! 4'+%2C!O:! *82!40(%+1>*&0(!0;! *0L&4! %82--;&%8!

*8'*!8'/2!'44+1+-'*2C!%'L&*0L&(!J37PK!'(C!&*%!C2,&/'*&/2%!O:!&(D2%*&0(!0;!*0L&4!+(&42--+-',!>8:*0>-'(24&2%! 0;!

C&'(0;-'D2-'*2%! '(C! >,024&2%H! R(! *82! 0*82,! 8'(C6!

S0C'1'! 2*! '-H! JTUVUK! ,2>0,*2C! *8'*! '! O'4*2,&+1! JI7AWTK! &%0-'*2C! ;,01! '! *0L&4! %*,'&(! 0;!!""#$%&'()*+,

-%+%(#&.#!'-%0!>,0C+42%!%'L&*0L&(!6!*80+D8!*82!:&2-C!&%!/2,:!-0MH!70L&(!>,0C+4*&0(!0;!*82!O'4*2,&+1!M'%!

0O%2,/2C!&(!-'*2!%*'*&0(',:!>8'%26!%+DD2%*&(D!*8'*!*82!*0L&(%!',2!4'*'O0-&Q2%!0;!%012!O'4*2,&'-!%+O%*'(42H!

X242(*-:6!M2!8'/2!;0+(C!*8'*!0+,!%>24&;&4!'(*&O0C:!*0!37P!,2'4*%!M&*8!X)$!0;!I7AWT6!%+DD2%*&(D!*8'*!*82!

X)$! 40+-C! O2! *82! 0,&D&(! 0;! 37PH! N*!M'%! %+>>0,*2C! O:! *82! ;'4*%! *8'*! 37P! &%! ,2-2'%2C! ;,01! *82! X)$!O:!

X)'%2! C&D2%*&0(! J+(>+O-&%82C! C'*'KH! 782%2! ;&(C&(D%! %*,0(D-:! %+DD2%*! *8'*! I7AWT! >0%%2%%2%! *82! D2(2%!

&(/0-/2C! &(! *82! O&0%:(*82%&%! 0;! I3I! *0L&(%H! $! >',*! 0;! *82! ,2%+-*%! 0(! *82! D2(2! .$-/! 2(40C&(D! '(! RW

4',O'10:-*,'(%;2,'%2!JR57$3.K6!*82!2(Q:12!*8'*!&(/0-/2%!&(!*82!;&('-!>,042%%!0;!37P!%:(*82%&%!&(!I7AWT!

M&--!O2!%80M(!82,2H!

D,%I/#8*L)I3I!*0L&(6!37PW>,0C+4&(D!O'4*2,&+1!I7AWT6!X)$6!%L*N6!RW4',O'10:*,'(%;2,'%2H)!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&$'!"#(!)#$*$#+(%,(-./.)#.'(.'$0/.(12-34%%1(.!)#-(%0#!$".'(,4%1(

'$,,.4."#(.!)#!"#-(

$%&&'(%!)**&(*&+#,",!$%&-'.*/!0/%123%4'5

#,"!%&4!6*&+572!$'/%'28&

#,"9!

#:72%35.7&5!*;!74!N584'7(,!0%(75(%35!?&'@73('51,!)%&+H*H,!#BIBB!

JKLMFLC:!OGLNL*&(8.74!'&!J=%'/%&4!487!5*!5=7'3!%@%'/%T'/'51!

5=3*8+=*85!5=7!17%3E!!K*-7@73,!/'55/7!'(!H&*-&!%T*85!5=7'3!;8&>5'*&%/!23*2735'7(E!!)1!8('&+!5=377!

4';;737&5!7V53%>5%&5(!O-%573,!ABW!75=%&*/!O@X@Q!%&4!4'75=1/!75=73Q,!5=7!7V53%>5(!;3*.!(7/7>574!

.8(=3**.(!(%.2/7(!-737!*T5%'&74!%&4!47573.'&74!;*3!5=7'3!;8&>5'*&%/!>*.2*&7&5(E!5'@'51,!3748>'&+!2*-73,!>=7/%5'&+!

7;;7>5!*&!;733*8(!'3*&(!%&4!(8273*V'47!%&'*&!3%4'>%/!(>%@7&+'&+!%>5'@'51!-737!8(74,!7.2/*1'&+!)KL,!YP

5*>*2=73*/!%&4!Z:JL!%(!2*('5'@7!>*&53*/(E!D7(8/5(!(=*-74!5=%5!5=7!ABW!75=%&*/'>!7V53%>5!*;!43'74!!5(

")*)"'!>*&5%'&74!;8&>5'*&%/!>*.2*&7&5(!='+=73!5=%&!5=*(7!*;!5=7!;*83!*5=73(!'&>/84'&+!5*5%/!2=7&*/'>!

>*.2*8&4(!O"[E"A!\!BE[I!.+]LZX!+!43'74!.8(=3**.Q,!5*5%/!;/%@*&*'4!>*&>7&53%5'*&(!O#E^[!\!BE_^!

.+`ZX+!43'74!.8(=3**.Q!%&4!5=7!%(>*3T'>!%>'4!>*&57&5!O[Ea_!.+X!#BB+QE!!Z@%/8%574!T1!:66K!%((%1!%5!

ABB!b+!./P#,!'5(!(>%@7&+'&+!7;;7>5!-%(!='+=73!O^[E_[WQ!5=%&!5=%5!-%(!;3*.!)KL!OA"E[[WQ!T85!/*-73!5=%&!

5=%5!-%(!;3*.!YP5*>*2=73*/!OIAEIIWQE!!K*-7@73,!5='(!7V53%>5!7V='T'574!/*-73!3748>'&+!2*-73!OBEBcQ!%(!

>*.2%374!5*!5=%5!*;!)KL!OBEa^Q!%&4!YP5*>*2=73*/!O#E#"Q!%5!ABB!b+!./P#E!G=7/%5'&+!%T'/'51!*;!5=7!ABW!

75=%&*/'>!7V53%>5!*;!43'74(!5(")*)"'!-%(!='+=73!Oa[E"aWQ!5=%&!5=%5!;3*.!Z:JL!OcaE^[WQ!%5!ABB!b+!./P#E!!

d&!5=7!>*&53%31,!'5(!3%4'>%/!(>%@7&+'&+!%>5'@'51!47573.'&74!T1!(8273*V'47!%&'*&!-%(!/*-73!O^[E#cWQ!5=%&!

5=%5!-%(!;3*.!)KL!OcAWQ!%&4!YP5*>*2=73*/!OI#EIcWQE!!M&!>*&>/8('*&,!5=7!75=%&*/'>!7V53%>5!*;!!5(")*)"'!

>*8/4!T7!8(74!%(!5=7!2*57&5'%/!&%583%/!%&5'*V'4%5'@7!(*83>7!*3!'&+374'7&5(!'&!;'(=!%&4!;'(=731!23*48>5!

'&48(53'7(E!

!

56789:;


7 December 2012,


Tokyo, Japan !

! ""!

!"#$%&'()*$+,"),!-&.+"/0)$/&'1&'()*$+,(%&-*+(,!-&,-"%(*$/&2+"3&*4(,&2$+3$!*$/&

2,-4&.+"/0)*-&

!

#$%&'()!*+,-+#./01!234(567!89/:;

"1!-?-@-

"!3%A!>(%B7!C;/;@;2;

"!!

!0:()3='D(%'!$E!F756(=G!#=$AHI'51!F3IHJ'G!$E!F756(=7(51!>35('53='!.%7K(=57'G1!L3%&4$4!0MNMM1!2637J3%AO!

":()3='D(%'!$E!L7$5I7(%I(!3%A!L7$'(I6%$J$&G1!F3IHJ'G!$E!-&=7IHJ'H=(1>GH56H!.%7K(=57'G1!FH4H$43!P0"QPRP01!e.!0]0!

)=$AHI(A!3I'7K(!)()'7A(!V7'6!@*!]MMRO!@$5'JG!3D7%$!3I7A!5([H(%I(5!$E!3I'7K(!)()'7A(!3=(!

6$D$J$&G!'$!/H43I7%!+C>Q0!Y@*!"N_MZ!)=$AHI(A!XG!6$,2073'('(()*!8,%&-!+C>Q01!(fI()'!

5(=7%(!3%A!K3J7%(!3'!)$57'7$%!0P!3%A!"0!$E!/H43I7%!+C>Q0!V(=(!=()J3I(A!XG!'6=($%7%(!3%A!

D('67$%7%(O!

T$%5([H(%'JG1!3JJ!$E!'6(!75$J3'7%&!5'=37%5!3%A!'6(7=!X3I'(=7$I7%5!3=(!)$'(%'JG!H5(A!7%!

EH='6(=!5'HAG!3%A!3J5$!7%!E$$A!)=(5(=K3'7$%!3))J7I3'7$%O!

&

56789:;


7 December 2012,


Tokyo, Japan !

! "#!

!

!

!

!

!

!

!

!

!

!

!

!

!

!

POSTERS

!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%"&%'"&"()*#+*,-(."/,(0*%,1*%2",#*%3"10*",*3#.,*4.#(/",*3#,3/,(.%(/*5"(6*.%",'#5*(.#-(*

7!"#$%&'"#&()*+',-))8*

9#.%(%(*:.%36#2;%(#6

;


7 December 2012,


Tokyo, Japan !

! "#!

Replacement of fish meal with rendered animal protein and plant protein sources on growth

and biological performance of rainbow trout

!"#$%&'()%*)%+,'-.,-%+/01,

()%2'0/3/%4/$/

(%

$%&'(&)*!+,-../!.0!1&%23*!+,2*3,*!&3'!4*,-3./.567!4.86.!932:*%;2)6!.0!1&%23*!+,2*3,*!&3'!4*,-3./.567!#>?!

4.86.!@AB>B=??7!C&D&3!

EF.%%*;D.3'235!&()-.%G!/0HI"@JK6&-..L,.LMD!

N3! &O(&,(/)(%*7! 02;-! P*&/! 2;! (;*'! &;! &! P&M.%! 235%*'2*3)! 0.%! D%.)*23! ;.(%,*! 23! )-*! Q.%/'L!

R.Q*:*%7! )-*!,.;)!.0! 02;-P*&/!&3'!)-*!3*5&)2:*! 2PD&,)!.3!)-*!*3:2%.3P*3)!.0! 02;-! 0&%P235!,.(/'!

/2P2)! 0(%)-*%! 5%.Q)-!.0! &O(&,(/)(%*L!9;235! )-*! 02;-!P*&/! &/;.! %*;(/);! 23!-25-*%!Q&;)*!.()D()L!4-2;!

;2)(&)2.3!)-*%*0.%*!;)2P(/&)*;!%*;*&%,-*%;!).!%*D/&,*!02;-P*&/L!S*3'*%*'!&32P&/!D%.)*23!235%*'2*3);7!

D.(/)%6! T6>D%.'(,);! P*&/7! 0*&)-*%! P*&/! &3'! T/..'! P*&/7! &%*! D.)*3)2&/! 02;-! P*&/! ;(T;)2)()*;! 0.%!

0.%P(/&)235! 02;-! '2*)L! U2))/*! 230.%P&)2.3! 2;! &:&2/&T/*! .3! )-*! (;*! .0! %*3'*%*'! &32P&/! D%.)*23! 23!

%&23T.Q! )%.()L! 4-*%*0.%*7! )-*! &2P! .0! )-2;! ;)('6! Q&;! ).! *V&P23*! )-*! *00*,)! .0! D&%)2&/! &3'! ).)&/!

%*D/&,*P*3)!.0!02;-!P*&/!Q2)-!%*3'*%*'!&32P&/!D%.)*23!&3'!D/&3)!D%.)*23!.3!5%.Q)-!&3'!T2./.52,&/!

D*%0.%P&3,*!.0!%&23T.Q!)%.()L!

W!@">Q**8! 0**'235! )%2&/!Q&;!,&%%2*'!.()! ).! 23:*;)25&)*!D&%)2&/! &3'! ).)&/! %*D/&,*P*3)!.0! 02;-!

P*&/!Q2)-!D.(/)%6!T6>D%.'(,)!P*&/!XYZ1[7!0*&)-*%!P*&/!X\]1[7!T/..'!P*&/!XZ1[7!'*0&))*'!;.6T*&3!

P*&/!X^+1[!&3'!,.%3!5/()*3!P*&/!XF$1[!23!D%&,)2,&/!'2*);!0.%!%&23T.Q!)%.()L!^(D/2,&)*!)%*&)P*3);!

.0!%&23T.Q!)%.()!XP*&3!232)2&/!Q*25-)_!@IL?`AL@5[!Q*%*!0*'!;2V!2;.32)%.5*3.(;!X=aL?b!,%('*!D%.)*23[!

'2*);L!R25-! 02;-!P*&/! '2*)! X#Ib!&3,-.:6!P*&/[!Q&;!'*;253&)*'!&;! ,.3)%./L! N3! )-*!.)-*%! 02:*!'2*);7!

?#b! &3'! @AAb! .0! 02;-! P*&/! Q&;! %*D/&,*'! T6! ,.PT23&)2.3! .0! YZ1c\]1cZ1! X\1cY\Z7! Y\Z[7!

YZ1c\]1cZ1c^+1cF$1!X\1cY\Z^F7!Y\Z^F[!.%!&!T/*3'!.0!^+1!&3'!F$1!X\1c^F[L!

\2;-!0*'!)-*!'2*);!)-&)!%*D/&,*'!02;-!P*&/!Q2)-!)-*!,.PT23&)2.3!.0!YZ1c\]1cZ1!&)!/*:*/;!.0!

?#b!&3'!@AAb!;-.Q*'!T*))*%!.%!;2P2/&%!Q*25-)!5&23!Xd$[!&3'!;D*,202,!5%.Q)-!%&)*!X+$S[!,.PD&%*'!

Q2)-!02;-!0*'!)-*!,.3)%./!'2*)L!R.Q*:*%7!023&/!Q*25-)!&3'!+$S!Q*%*!/.Q*%!Q-*3!02;-!0*'!)-*!\1c^F!

&3'! Y\Z^F! '2*);! )-&3! )-*! .)-*%! )%*&)P*3);7! T()! 3.)! ;253202,&3)/6! '200*%*3)! 0%.P! )-*! 02;-! 0*'! )-*!

,.3)%./! '2*)! X!eALA#[L! \2;-! 0*'! )-*! ,.3)%./! '2*)! ;-.Q*'! )-*! /.Q*%! 0**'! 23)&8*! )-&3! 02;-! 0*'! )-*!

\1cY\Z!&3'!\1cY\Z^F!'2*);!X!fALA#[7!&3'!;-.Q*'!)-*!T*))*%!0**'!,.3:*%;2.3!%&)2.!)-&3!)-*!.)-*%!

)%*&)P*3);! X!fALA#[L! 4-*%*! Q*%*! 3.! ;253202,&3)! '200*%*3,*;! X!eALA#[! 23! )-*! -*P&).,%2)! &3'!

-*P.5/.T23!&P.35!02;-!0*'!)-*!*VD*%2P*3)&/!'2*);L!4-*;*!%*;(/);!23'2,&)*!)-&)!)-*!,.PT23&)2.3!.0!

YZ17!\]1!&3'!Z1!,.(/'!T*!T*))*%!D%.)*23!;.(%,*;!)-&3!)-*!^+1!&3'!F$1L!N)!Q&;!&/;.!;(55*;)*'!

)-&)!)-*6!,&3!T*!(;*'!23!%&23T.Q!)%.()!'2*);!&)!-25-!23,/(;2.3!/*:*/;L!

%

5"67189:;%S*3'*%*'!&32P&/!D%.)*237!D/&3)!D%.)*237!D%.)*23!()2/2g&)2.37!-*P&)./.567!%&23T.Q!

)%.()!


7 December 2012,


Tokyo, Japan !

! "#!

!"#"$%&'(#&)'*)+*#,"*-."#/('0*1"0&%"'#1*(2#&'3*(1*4&)$"(2#)$15*,67)#,"1&1*

+$)%*#,"*1#('07)&'#*)+*#,"*%"2,('&1%*)+*8"//9/)1"*:&'0&'3*!)%(&'*;8:!"'*?&9@A*B,&'3)*C("3(.(

DA*E($9,&=)*F)6),($(

@*G*

!

$%&'&(&)*!)+!,--.&/0!1&)(2&/*2/(3!4560768/!92:)).!)+!,;5&27.875/3!*&'/5(&8=3!


7 December 2012,


Tokyo, Japan !

! "#!

!"#"$%&'"()*%+*),"*-"./$0(1"2*!34*'561"6*0(*7%''%(*756&*8!"#$%&'()*+$#%,9*

:*;5(?5*@*A;*A5)%

B@*C;*=0?>7,0

B@*D;*=0650

7*5(2*C;*3515E0'5

*5� *

5F*!"&56)'"()*%+

*C560("*G0+"*A70"(7"*5(2*H%&>$5)0%(*I"(")07*J(+%6'5)07-@*I652>5)"*A7,%%$*%+*4?607>$)>6"*A70"(7"@*D%,%1>*

K(0#"6-0)L@*A"(250@*C0L5?0@*M5&5(*

BF 300?5)5*H6"+"7)>65$*J($5(2*N5)"6*O0-,"60"-*P.&"60'"()*A)5)0%(@*35?5%15@*300?5)5@*M5&5(*

7F*!"&56)'"()*%+*G0+"*Q*="5$),*A70"(7"@*D"01L%*K(0#"6-0)L*%+*A70"(7"@*K"(%,565@*:5'5(5-,0@*M5&5(*

RS%66"-&%(20(?*5>),%6F*H6%+"--%6*C5-5'07,0*3515E0'5@*!"&56)'"()*%+*C560("*G0+"*A70"(7"*5(2*H%&>$5)0%(*I"(")07*

J(+%6'5)07-@*I652>5)"*A7,%%$*%+*4?607>$)>6"*-70"(7"@*D%,%1>*K(0#"6-0)L@*D->)->'02%60/4'5'0L5@*4%B5/T>@*A"(250@*UVW/VXXX*

M5&5(;*

P/'50$F!$%&'$()*+,-.+/+&0-12-'3!

!

405!.+!./5!6*77585%.!98+:./!81.5!)5.:55%!$1;5!1%6!75$1;5!2+$$+%!2183

3+30;1.*+%!:*;;!)5!16=1%.195+0,!.+!2+$$+%!2183!20;.085-!>%!./5!;1,.!652165,

.52/%*?05!+7!./5!@1%6+$!A$3;*7*56!B+;C$+83/*2!4DA!E@AB4F!7*%95838*%.*%9!38+=*65!1!0,570;!.++;!.+!

1$3;*7C!0%&%+:%!4DA!,5?05%25,!)C!0,*%9!,*%9;5%!./*,!

2+%%52.*+%


7 December 2012,


Tokyo, Japan !

! "#!

!"#"$%&'())"&$'*)'+","-."/'0-.1'2-,3*#'!"#$%&'()'*#'4%,/'5*61,-$%*#.''

!7-8-)13%'0%8-3%

9':;'0-.-$*'2-$*

<'-#/'0-.-3%&=%'>-8-?%3-

9''

!$ %&'()'*+!,-.//0!/1!23&4-)0*)&'0!,-4+5-+!6'-)0*7!/1!23&4-)0*)&+8!9/./:)!;54$89=)*=)?4(/&4'?'?47'?'-.48!2/@'>:)!,+5('4>=.48!A47'348!B#$>#CCC8!D'E'5!" 254!@&'5-.8!2:4*'!F&+1+*)&'0!64=.+&4+=!G+5*+&8!2H'*/:)='H'I'JK8!254!L':'?)&'8!M4*'':4*'>=.48!2:4*'8!N$#>OKP"8!D'E'5!!

!

QG/&&+=E/5(453!')*./&R!*>?4:'?4S@4/=T*/./:)T'-TUE!

!

!

A'=)!='0?/5!"#$%&'()$%(*+!,-+&*.!4=!/5+!/1!-/??+&-4'007!4?E/&*'5*!=E+-4+=!45!D'E'5T!

V/I+Y404001!#>>04?>08/8.8!I.4-.!I+&+!(+,1/&H'!'5(!](I'&(=!-./&(!(4=*'5-+=!I'=!3+5+&'*+(!I4*.!

F_F;`29W_L,!$T"TP"T!25!'==435?+5*!*+=*!I'=!-'&&4+(!/)*!)=453!%]L]G`2,,!"T!

200+04-!&4-.5+==!&+


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&'#(#))#%*(+,('-&..&,/(0#$)+$.1,%#(+)(21%3(.1%3#$#4(!"#$%&"&'()#*+,-$&'5(#"1.&,#6(78(

.9'%4#(*-&*%:(#"0#$&.#,*(

!

;+%:1..16(131)9.&(?$&.+*+

@A(

!

$!%&'()'*+!,-)&.+!-/!01123+(!4'&35+!63-.73+57+.8!9-:;-!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&'&()%&*$+*'+,--.+/*0*1*23#4+ &$+ %/#+ 2#$*0&(+567+*'+ 83930)+ 4/9&0:;+!"#$%&'("'%$)

*"&+(,-%$+

7&8*+ -/&%)9))8)4/&

+?*@)0)

<;+ ?)$)+A*%*

<;+ B3@)+ -/&2#$*C3

D;+ >)830)+-32)@)

D;+E*%*/&8*+

-)$*D;+F&"#/&9*+?*$"*

<;+!83*+F&9*$*

!

#M'((-! 1'5.)(2-! )6-! ! '+! )6-2(! 2//5.-! -.'/-H! 86--.'/21!

cNJ! '+! 95(5/%!

%7-&-)R--.! )6-2(! >-.'/21! cNJ

-++212-.1*!'+!;2(5-.-

!?#@G*9"4H+$)/+*0'()'*+"%)0-(12*+3"Z00\3!Z00\!6'/'7'>5-+


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&&'()*+,-'.+(/$#00#1/$)0$%(/#23#'4%(*56$%($7+8+(#9#$03)'(,#2$:!"#"$%&'(')*+,$%-"&./*;$$

$?4')$@%2)()>$@%,#"%2)$A)(,)$

$%&'(%)'(*!'+!,-.'/-!012-.1-3!4'5*'!6.27-(82)*!'+!9%(2.-!012-.1-!%.:!4-1;.'


7 December 2012,


Tokyo, Japan !

! "#!

!"#$% "&%!"#$%&'("'%$) *"&+(,-%$) '()*+,-.#,/$% 0$0+,1$% 232,-*+% .,/#,+) &'("',-,0"% 2-1% 45,+$%

*0"+%6,()*%,-&$'+,"-%

$%&'())!*+,-)+.-/!*+0&%+'-!1-20-/!345-!*+'-2-6!

789-'8.-'(!-:!;&2--4(-!?2+@&'A+.(!-:!B8'+2&!$=+&2=&!820!>&=%2-)-C(!!

1-282!DEFEG/!B+28.-/!HIJEJDGG/!>-4(-/!K8,82!

!6L-''&A,-20+2C!85.%-'M!%+'-2-N48+(-08+O8=OP,!

!

32! 82! &2@+'-2-:!GEHD!4X8/!Q+.%!8!=%8'8=.&'+A.+=!YRU!0-%&!'&A5).A!0&


7 December 2012,


Tokyo, Japan !

! ""!

!"#$%&'()*)(+),,-#'+.(/)0./)"#+.1.)#%/+2"-34'+%/5.#2'2+6!7+)#+8-5-,.+%95),&:++

!"#$%&'("'%$)*"&+(,-%$)

#$%!&'%()*!+,-).,/%!0%'-%*!&1(%!+,/%'%!

234%/35%/$!%6!7)'%8)!9:,)':)*!;%1$%!./,8D!,>!,8D%/53'5!3>!3!6,>.)/$!/)>%(/:)!3'-*!,>!:(@5,=35)-!3/%('-!5.)!I%/@-F!+%I)=)/*!

5.),/! ,88(')!>$>5)8! ,>!'%5! 6(@@$!('-)/>5%%-!3'-! ,5! ,>!'):)>>3/$! 5%!A3,'!8%/)! ,'6%/835,%'F! &'!1(/(83!

>./,8D*!#JK!,'5)/6)/)':)!L#JK,M!:3'!4)!366):5)-!)3>,@$!4$!-%(4@)!>5/3'-!#JK!L->#JKMF!;.)/)6%/)*!->#JK!

.3>!4))'!(>)-! 6%/! 3!'(84)/!%6! A)')!1'%:1!-%I'!)ND)/,8)'5>! 3'-! /)D%/5)-! ,'O):5,%'!%6!'%'P>D):,6,:!

->#JK! ,':/)3>)>! 5.)! >./,8DQ>! 34,@,5$! 5%! -)6)'-! ,5>)@6! 3A3,'>5! ,'6):5,%'F! K::%/-,'A@$! I)! D)/6%/8)-!

8,:/%3//3$!3'3@$>,>!5%!1'%I!5.)!:%8D/).)'>,=)!A)')!)ND/)>>,%'!3'-!5%!-)5)/8,')!I.,:.!A)')>!/)@35)!

5%!5.,>!D.)'%8)'%'F! !;.)!3//3$!>@,-)!I3>!:%8D%>)-!%6!RSTU!>)V()':)>!5.35! ,':@(-)-!1(/(83!>./,8D!

8#JK>*! W9;>*!X.,5)! >D%5! >$'-/%8)!=,/(>! LX99YM!.%8%@%A%(>! A)')>! 3'-!X99Y!A)')! >)V()':)>F! ;.)!

>38D@)>!I)/)!D/)D3/)-!I,5.!,'O):5,%'!%6!->#JK!53/A)5,'A!A/))'!6@(%/)>:)'5!D/%5),'!L7ZHM!A)')!L->7ZHM!

5%!1(/(83!>./,8DF!+3)8%:$5)>!I)/)!>38D@)-!35!"*!S*![U!3'-!U\!.%(/>!D%>5!,'O):5,%'!I,5.!H]9P,'O):5)-!

A/%(D!3>!:%'5/%@F!X)!5.)'!3'3@$^)-!5.)!)ND/)>>,%'!%6!>%8)!A)')>!,'!-)53,@!(>,'A!#;PHC#!3'-!/)3@P5,8)!

HC#F!!Z%/5$P>)=)'!LU_M!A)')>!>.%I)-!U!6%@-!)ND/)>>,%'!:.3'A)>!3A3,'>5!H]9!,'O):5)-!A/%(DF!X)!>)@):5)-!

[!A)')>!6/%8!5.)!:@(>5)/*!>.%I,'A!(D!/)A(@35,%'!35![U!3'-!U\!.%(/>!D%>5!,'O):5,%'!6%/!6(/5.)/!3'3@$>,>F!

H/)-,:5)-! 38,'%! 3:,-! >)V()':)>! %6! )3:.! A)')! .3-! 3DD/%NF! U`a! 3'-! "`a! ,-)'5,5,)>! I,5.! A/3'(@,'!

D/):(/>%/!3'-!.$D%5.)5,:3@!D/%5),'*!/)>D):5,=)@$F!;.)!5/3'>:/,D5>!%6!5.)>)!A)')>!I)/)!83,'@$!-)5):5)-!

6/%8!5.)!A,@@>*!.3)8%:$5)>*!.)3/5!3'-!,'5)>5,')F!K'-!X99Y!3'-!b)@@%I.)3-!=,/(>!Lb+YM!,'6):5,%'!,'-(:)-!

5.)>)!A)')>!)ND/)>>,%'*!.%I)=)/!!"#$"%&'()*("+",*! ,'6):5,%'!-,-'c5F!;.,>!/)>(@5!>(AA)>5>!5.35!5.)!J%'P

>D):,6,:!,88(')!3:5,=35,%'!,>!3!>$>5)8!5%!D/%5):5!>./,8D>!6/%8!=,/3@!,'6):5,%'F!!

;'


7 December 2012,


Tokyo, Japan !

! "#!

Change in immune gene expression pattern of koi herpesvirus infected! common carp, Cyprinus carpio

$%!&'(')'*'+!,%!&'-.+!/%!&.(0.+!1%!2.34!'(0!5%!/46.(.!!

!"#$%"&$%'($)(*+,$-+(./0+,/+1(*%"23"&+(./4$$5($)(6"%0,+(./0+,/+(",2(7+/4,$5$8'1(

7$9'$(:,0;+%AA1(7$9'$1(E"F",(

!

&.4!78698:;46?+!'!;46'@!9'-7.A8(!.B!C.DD.(!C'69+!C'!4(B8C-4.(!4(C@


7 December 2012,


Tokyo, Japan !

! "#!

!

"#$%&%'()*+!&,&-.')'!/0!'1+2)0)2!3+,+!+#14+''),3!),!$+5/2.(+!0/4!6&4'%1+,&+%'!7&1/,)2&'!8.!

6)24/&44&.!

$%&%!'()(*+!,-./*-0(!$(&.(+!123(!,-0(&(!

4%5(0%)(06!(7!'/&(8/!9:-/&:/+!;(26(!-)6!(7!?%0-&/!9:-/&:/!%&.!;/:*&(@(A6+!B%C%&!

D!E(00/>C(&.-&A!%3)*(0!C%C%0%226(((((FA8%-@G:(8!

,/8(:6)/!(7!?%0>3C/&%/3>!H%C(&-:%>!->!:@%>>-7-/.!"!)6C/>!I*/)*/0!)*/0/!%0/!A0%&3@/!(0!&()+!%&.!

>-J/! (7! A0%&3@/G! ;*/0/7(0/+! -)! ->! &()! />)%5@->*! 8(@/:3@%0! 5-(@(A-:%@! I%6! )(! :@%>>-76G! K/! &//.! )(! >(0)!

>C/:-7-:!A/&/>!/LC0/>>-&A!-&!>307%:/!(7!*/8(:6)/G!->!M9;!(5H/:)/.!(7!

?%0>3C/&%/3>! H%C(&-:%>! /%:*! (0A%&>! 3>-&A! N/L)! '/&/0%)-(&! 9/O3/&:/>! PN'9QG! 1&! )*->! >)3.6+! I/!

:(&>)03:)! &/I!(@-A(!8%-:0(%00%6! )-C>+! %&.!(5H/:)! )(! /LC@(0/! >C/:-7-:! A/&/!/LC0/>>-&A! -&! */8(:6)/! 7(0!

?%0>3C/&%/3>!H%C(&-:%>G1&!(0./0!)(!:(&>)03:)!(@-A(!RNS!8-:0(%00%6!)-C>+!I/!>/@/:)/.!TU+VW#!>/O3/&:/>!

70(8! M9;! %&%@6>->! 3>-&A! N'9! >/O3/&:-&A! I*-:*! %0/! (5)%-&/.! %5(3)! UU! 8-@@-(&! >/O3/&:/>! %&.! WX#!

>/O3/&:/>! (7! )0%&>:0-C)-(&! C0(.3:)>! 70(8! C35@-:! .%)%5%>/! -&7(08%)-(&G! K/! C/07(08/.! /L*%3>)-=/!

%&%@6>->!%5(3)!/LC0/>>-&A!A/&/>!(5H/:)/.!*/8(:6)/+!A-@@>+!*/C%)(C%&:0/%>+!@68C*(-.!(0A%&>!%&.!83>:@/!

(7! */%@)*6! >*0-8C>! 56! :(&>)03:)/.! 8-:0(%00%6! )-C>G! Y@-A(! RNS! 8-:0(%00%6! )-C>! I/0/! :(&>)03:)/.! 56!

70%A8/&)>!I*-:*!I/0/!(5)%-&/.!56!N'9!>/O3/&:-&A!%&.!*%.!*(8(@(A6!)(!()*/0!>C/:-/>G!!;*/!0/>3@)!(7!

%&%@6>->!(7!A/&/!/LC0/>>-(&! -&!/%:*!)->>3/!56!8-:0(%00%6+!%@)*(3A*!)*/!/LC0/>>-(&! @/=/@!(7!MZT[\!%>!%&!

-&)/0&%@! :(&)0(@! I%>! *-A*! -&! %@@! (0A%&+! )*/! /LC0/>>-(&! @/=/@! (7! @6>(J68/! %&.! :03>)-&! A/&/! -&! A-@@! %&.!

@68C*(-.!(0A%&>!I/0/!*-A*/0!)*%&!83>:@/!%&.!-&)/>)-&/G!K/!>/@/:)/.!UT!>/O3/&:/>]!)*/!/LC0/>>-(&!(7!

)*/>/! >/O3/&:/>! -&! */8(:6)/! I%>! T^! )-8/>! *-A*/0! )*%&! ()*/0! (0A%&>G! K/! %0/! &(I! 7(:3>-&A! )*/>/!

>/O3/&:/>+!%&.!%&%@6J-&A!)*/>/!A/&/>!-&!./)%-@G!

!

9+.:/4;'_!9*0-8C+!8-:0(%00%6+!*/8(:6)/!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$"%&'(%")*"$"+*"*,%"-(.&'/0+,01,/##(+")%".&'"*,-"+","2$%"33/0+,/+,1/34,5(.'(%",5"..36,

$%&'%()!*)()%+!,%-.'%/0!102-0+!3(40!,%/020!

5)60/)70/8!09!:.20;.!*&%.2&.+!:/)-4)7.!*&'00.&'200(80!@2%A./B%78!09!=)/%2.!*&%.2&.!)2-!

>.&'200(80!GHIDICFF+!J)K)2L!

M!N0//.BK02-%2?!)47'0/!?00B'00')O?;)%

7'.!?.2.!.UK/.BB%02!K/09%

7/.)7.-! T%7'! K0.;K./)74/.+!>8K.DG!%27./9./02!

!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$"%&'%()*+),-.'-/0")."('*%)*+)'11$%*(0*/$0'%)%"2)-%3'("%)."&"43*.)("%"5)'%)36")/-%7"7)

6*$%756-.89)!"#$%#&'&()**#+,!

!

$%&'()&*+!$+,%-.!/0+'!1)2'3'

-.!1),45)2'!6'3,'

-.!7%0%*5)!8'0)

9!

-:%;'2%&'2

JG'224*F'3,)3D!%+&5'2K!*+,%*%&'9-LD(%)CM?'(!

! G%2&)C%D)3'+*!=)*5!F'**4**!&54!)((+3'DC';+C)3!)*'&

*4D(43&*! %24! 24%22%3D4,M! I4! 5%A4! ?C'34,! &54! ?SP8! 43?',)3D! /DP8H! =2'(! &54! ;%3,4,! 5'+3,*5%20!

O!"#$%#&' &()**#+,Q! %3,! *5'N4,! &5%&! )&! ?%3! F2',+?4! %! A%2)4&

%2&)=)?)%C! *)3DC4! ?5%)3! %3&);',

A%2)%;C4! 24D)'3! =2'(! &54! ;%3,4,! 5'+3,*5%20M! V)2*&.! N4! 4A%C+%&4,! &54! 3+(;42! '=! /DP8H! D434! ;

$'+&5423!;C'&&)3DM!754!24*+C&!'=! &5)*!%3%C

!

:";2*.75K![%3,4,!5'+3,*5%20.!/((+3'DC';+C)3.!/DP8H.!R%2)%;C4!24D)'3!


7 December 2012,


Tokyo, Japan !

! "#!

!""#$%&'"&$'(')&'*%+,+-#(&./0&12$$+)#&232+)4%&!"#$%&'$()*%'"+$*(%'&2)%+3#)+$*6#44+')&+)&

72*2)#4#&"8'9)(#6:&,$%$+'#-.-/)("+'0$#*1)(

$%&'(%')&!*&+,-!.'/0%')&!1&2/&-!3+4&!.')&2&!

5,6&),7&)8!&9!:02&;0!$('02(0-!0)-! ,27'6'&7'(! 7%0),J8!%,?!,! )'?+!&9!/0>0A&J'2B!,27'6'&7'(! )0?'?7,27!6,(70)',!

,2/!7%'?!(,AA!9&)!7%0!/0>0A&J;027!&9!0990(7'>0!>,(('20?!,>&'/'2B!?4(%!)'?+O!*;&2B!24;0)&4?!>,(('2,7'&2!

?7),70B'0?-!WX*!>,(('20! '?!,2!,77),(7'>0!,JJ)&,(%O!WX*!>,(('20?!,)0!JA,?;'/!WX*!02(&/'2B!,27'B02!&9!

'290(7'&4?!J,7%&B02?!,)0!9A0Y'6A0!7&!/0?'B2!,2/!?,90!(&;J,)0/!7&!,77024,70/!A'>0!>,(('20!,2/!'2,(7'>,70/!

>,(('20O!WX*!>,(('20!Q&)+?!68!?7';4A,7'2B!0YJ)0??'&2!&9!,27'B02'(!J)&70'2!7%,7!'2/4(0?!,27'B02!?J0('9'(!

';;420! )0?J&2?0! ,970)! '2P0(7'&2O! .&Q0>0)-! WX*! >,(('20U?! ,6'A'78! Q,?! &9702! A';'70/! 68! )0?7)'(70/!

0YJ)0??'&2!&9!,27'B02'(!J)&70'2!/40!7&!/'990)02(0?!'2!(&/&2!4?,B0!68!7%0!J,7%&B02!,2/!%&?7O!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$ %&'()&*$ +,'-./-0&,'$ 1)2.'"-.,#-,#3$ 4#5.()6#57$ 8"#9-6&*$ 8"&)&6'#)-:&'-.,7$

&,0$+;;*-6&'-.,5$

!

!"#$

)-:-0!,'0!:8.(%-'!./-0,(-.'! -'! 2..01!,'0!3-.).4-&,)! 1*1(%51

-'!695,'1!,'0!,'-5,)1+!,'0!-(1!8.)%!-'!:8%7%'(-'4!./-0,(-7%!1(8%111&,7%'4-'4!&.5:.9'01!-'!&.5:)%/!5-/(98%1!,'0!-(1!,::)-&,(-.'!2.8!

0%(%&(-'4! %84.(6-.'%-'%! -'! 59168..5! 1,5:)%1! ;-))! ,)1.! 3%! :8%1%'(%0

&.'&%'(8,(-.'! .2! 3-.,&(-7%! &.5:.9'01! -1! 193G%&(! (.! (6%! -'2)9%'&%! .2! 5,'*! 2,&(.81+! 19&6! ,1! &9)(-7,8+!

%'7-8.'5%'(!,'0!:6%'.).4-&,)!1(,4%!098-'4!1,5:)-'46,87%1(! 1(.8,4%!,'0!:8.&%11-'4!.'! (6%!,'(-./-0,'(! &.'1(-(9%'(1!

I%84.(6-.'%-'%!,'0!:6%'.)-&1J!.2!%0-3)%!59168..51


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&'(#))*+,$+-$.*+/(0,)-+(10/*+,$20/"304$5#,#)$+-$6*7#$!*70'*0$8!"#$%&"$'()*+(,$-(%.)9$

:,;4$?#3?3*!

@A-?3.0B-*0!&1!CDE35E80E.-:!F35E80(!&1!F+/7-.+-/:!G3/-0/3.0!)*+,-./+0(:!H3*9'&'!$#I##:!%73+83*6!!

!

J!K&..-/?&*6+*9!3E07&.L!1E03B+M'3+(&63+N35NO?!

! %7-!E/39-!&1!B38357+0-!9.--*!+/!?.&7+P+0-6! +*!3DE35E80E.-N!Q&R-,-.:!07-.-! +/!3!?&//+P+8+0(!0730!

13.B-6! 1+/7! 3.-! -S?&/-6! 0&! 07-! .+/'! &1! 1--6! P&.*! 57-B+538! 5&*03B+*30+&*N! C50E388(:! 1+/7! B-38!

5&*03B+*30-6!R+07!B-03P&8+0-!1&.B!&1!2T!U8-E5&B38357+0-!9.--*V!73/!P--*!1&E*6N!%7-.-1&.-:!3!P-00-.!

E*6-./03*6+*9! &1! 07-! S-*&P+&0+5! B-03P&8+W+*9! -*W(B-/! UX2Y/V! 3*6! 6-0&S+1+530+&*


7 December 2012,


Tokyo, Japan !

! "#!

!"#$%&'()*$'*$'+&('#*+,#-%&'()*&'*%./.0$*"1/&02*!"#$%&'("'%$)*"&+(,-%$*

3$/&*4'$5$6)*7(&".%(*7&1$/$

8)*!,$%$*91&%&

8)*:-0-,$*7-'-

;)*:(/.#-,-*)*?$@$*9.51$%$/$'

A*$'5*:-"1&$%&*4#$0&

=)*B !

#$%&'()*+,*-.*%/(0!1(/)2/&'!3,455.!56!78(*,2.&2('!/%)!9%8*%''(*%8:!;%*/?*:!@/-/%!!

B$%&'()*+,*-.*%/(0!C'+'/(,4!D(8/%*>/&*5%:!;%*/?*K2S/,ST-!

! $%!


7 December 2012,


Tokyo, Japan !

! "#!

!"##$"%&'(%)$*#+#"%,'-+$#+./0#1$'+$23"30%$14"'05$!"#$%&'("'%$)*"&+(,-%$$

6%"'$7+%&%89$:#'132#$:'4%"%

;9$-0-/%$:-+-

?9$>#"3,-/-$@-14'&%

A9$6%1%4'"-$=%2%'

A9$>-4"3$

6#2%,%B9$C%D%$=3&4%2%"%+

E$%+&$>-14'%2'$7,%0'

A9$F !

$%&'()*+,-+./+&0)1!2)0*30'(!4-566/!67!89)+-3/'3)(!0&*!:&9+&(()+&9;!+10?0@+;!A0.0&!!

B%&'()*+,-+./+&0)1!C(,(0)-5!D)90&+?0'+6&;!+10?0@+;!A0.0&!

FG0'+6&0/!C(,(0)-5!%&,'+'3'(!67!8H30-3/'3)(;!D+'0;!A0.0&!

I4-566/!67!J+6K4-+(&-(,!0&*!L(-5&6/691;!M%L!


7 December 2012,


Tokyo, Japan !

! "#!

!"#$#%&'$()#&(*+,*-,.*//,(+&'$#%&(+0,1$*&'(+,0'+'2,!".*//(12,(+,34$45#,6"$(51,7!#$%&'()#(&%*

"#'+),-&%8,

9'(643',9("#$#:2,;#$(,2,?@#3#,6"(3(

:2,.*5*@#,9*+*

A2,.'$4&*@*,B*6"(=#

C2,;#6#"($*,D#3#(

C2,#+=,

.*6"(#3(,'?'7!!

@A7*+&(49-4?047'&:!%&'()'*+!,-.//0!/1!23&4-)0*)&+!'7(!B7347++&4735!6748+&94*:!/1!;4:''?'7!

!

FG/&&+9?/7(473!')*./&H!4*'I4*J--KI4:'


7 December 2012,


Tokyo, Japan !

! ""!

!"#$%&&'()*+)+,-&'&*(.*'//0)(1$%,+2%3*4%)%&*30$')4*5667*').%82'()*')*90$0/+*&:$'/#;*

!"#$%&'("'%$)*"&+(,-%$*

06&0;(04%/*!?/%>'%)1!@&A++(!+,!-./0&'()'/1!%4>!B4.0411/04.2!34051/60)*!+,!70*%8%902!:%;%4!!

C?/%>'%)1!@&A++(!+,!-./0&'()'/12!34051/60)*!+,!70*%8%902!:%;%4!!

"=4)1/>06&0;(04%/*!D161%/&A!E/.%408%)0+42!34051/60)*!+,!70*%8%902!:%;%4!

FG%)0+4%(!D161%/&A!=46)0)')1!+,!-H'%&'()'/12!E0)%2!:%;%4!

I@&A++(!+,!J0+K@&014&16!%4>!L1&A4+(+.*2!M=L!34051/60)*2!=4>0%!

N@&014&1!O14)1/!+,!L14610!@'06%42!@%.%2!:%;%4!

!

PO+//16;+4>04.!%')A+/Q!0)%R0)S&&TR0*%8%90K'T%&TU;!

! -;+;)+6062!%!;/+./%RR1>!&1((!>1%)A2!06!94+V4!%6!%!>1,1461!R1&A%406R!%.%046)!50/%(!04,1&)0+4!04!

%40R%(6T! W+V151/2! )A1! %;+;)+606! R1&A%406R6! +,! 6A/0R;! %/1! 4+)! V1((! '4>1/6)++>T! =4! R%RR%(62!

&*)+&A/+R1!&!06!/1(1%61>!,/+R!R0)+&A+4>/0%!%,)1/!)A1!50/%(!04,1&)0+4!%4>!,+/R6!%!&+R;(1X!V0)A!%;+;)+)0&!

;/+)1%61K%&)05%)04.! ,%&)+/! #! %4>! &%6;%61KYT! LA1! %&)05%)1>! &%6;%61KY! %&)05%)16! 1,,1&)+/! &%6;%612!VA0&A!

04>'&16!%;+;)+606T!!-;+;)+606K/1(%)1>!.1416!Z!"#T2!-@[#2!:G[2!;C\!;FC2!&*)+&A/+R1!&!%4>!=-]^!A%51!_114!

0>14)0,01>!04!9'/'R%!6A/0R;T!-,)1/!)A1!%/)0,0&0%(!04,1&)0+4!V0)A!`@@M2!&%6;%61!.141!1X;/1660+4!>1&/1%61>!

%4>! =-]! Z04A0_0)+/! +,! %;+;)+606^! .141! 04&/1%61>2! )A'6! )A1! %;+;)+606!V%6! 6';;/1661>! %4>!`@@M! &+'(>!

/1;(0&%)1! 04! )A1!&1((6T! ! :-[a@L-L!60.4%(04.!;%)AV%*! 06! 94+V4!%6!%4! 0R;+/)%4)!>1,1461! 6*6)1R!%4>! )A06!

;%)AV%*! 06! 6';;/1661>! _*! @EO@! 04! R%RR%(T! @L-L! %4>! @EO@! .1416! 04! 9'/'R%! 6A/0R;! A%51! _114!

0>14)0,01>T! ! -,)1/! )A1! `@@M! 04,1&)0+42! @L-L! .141! 04&/1%61>! 04! !04!"\!A!%4>!/1%&A1>!%)!)A1!1X;/1660+4!(151(!+,!b#c!A+(>!A0.A1/!)A%4!)A1!

040)0%(!1X;/1660+4!(151(!04!F!>%*6T![4+&9>+V4!+,!@L-L!.141!,+((+V1>!_*!`@@M!04,1&)0+4!&%'61>!)A1!/%;0>!

%4>! 6151/1!R+/)%(0)*T! ! DG-! 60(14&04.! ZDG-0^! 06! %! &+RR+4! %4)050/%(! >1,1461!R1&A%406R! 04! 1'9%/*+)16!

,/+R! ;(%4)6! )+! %40R%(6T! DG-0! 06! ,'4&)0+404.! )A/+'.A! )A1! 61H'14&1K6;1&0,0&! 6R%((! DG-T! DG-K04>'&1>!

60(14&04.! &+R;(1X! ZD=@O^! /1&+.40816! )A1! )%/.1)! RDG-! %4>! &%'616! >1./%>%)0+4! %4>! )/%46(%)0+4%(!

/1;/1660+4T!DG-0K/1(%)1>!.1416!Z!"#T2!d0&1/#2!d0&1/!! &A%/%&)1/081>! 04! 9'/'R%! 6A/0R;T! [4+&9>+V4! +,! -/.+4%')1! _*! `@@M! %/)0,0&0%(!

04,1&)0+4! &%'61>! )A1! 1%/(01/! +461)! +,!R+/)%(0)*! )A%4! )A1! &+4)/+(T! =4! )A1! ;/1614)! 6)'>*2!V1! /1;+/)! )A1!

04)1/%&)0+4!_1)V114!`@@M!04,1&)0+4!%4>!)A161!0RR'41K/1(%)1>!.1416!04!9'/'R%!6A/0R;T!

!

F%-N($3&P!$%&'()!*%!('+,%)-*./%'2!%;+;)+6062!:-[a@L-L!60.4%(04.!;%)AV%*0 DG-0K/1(%)1>!.14162!.141!

1X;/1660+4!%4%(*6062!`@@M*

!


7 December 2012,


Tokyo, Japan !

! "#!

!" #$%" &'(#)*$'(+,$" -.,&/0'.12'$)/)&(#3$" 4,()-/0)" 0$'/5/#1" *'6-" 7.-(#" $#&$'/3" 6'"

$#5/'6#-$#&(," -/3'66'1(#/)-)8" 0/)365$'/#1" *'6-" !"#$%$&'() *+,#$-*./'" /#" &/,(4/(" *('-9""

:7(/,(#0"

"

:(;()7/" !6;/(#1

=9" :($2A.#1" >.#1

=9" >(#$#.B" C(#1&(5(&37(/

D9" ?('.;6"

:(;$E(-(F9"?/0$7/'6"G6#06

H"(#0"I;.6"?/'6#6

HJ"

"

$%&'(&)*+,-.+!/.(.,012! 3'(-*-4-.! 5&0!6+7,'1.+! 81*.'1.!,'+!9.+*1,)! %,0.:!;,(.+,!

%24),)&'E?&0'!2,*),'+:!"N.A,0-K.'-! &5! P*5.! 81*.'1.! ,'+!9.+*1,)! D*&(1*.'1.:!;,(.+,!

&?=&! $RBQS"ST:! @,A,':!#P,U&0,-&0=! &5! I.'&K.! 81*.'1.:! >&?=&!

.12'&)&E=:!>&?=&:!@,A,'!

!

6'-*U*&-*1(! 2,+! A0.7*&4()=! U..'! 1&KK&')=! 4(.+! *'! 5*(2! 14)-40.! -&! 1&'-0&)! U,1-.0*,)! ,'+! K=1&-*1!

*'5.1-*&'(V! W&X.7.0:! -2*(! A0,1-*1.! 2,+! ).+! -&! -2.! .K.0E.'1.! &5! ,'-*U*&-*1! 0.(*(-,'-! U,1-.0*,! X2*12! *(!

&5-.'!5,1*)*-,-.+!U=!-2.!2&0*Y&'-,)!E.'.!-0,'(5.0!&5!E.'.-*1!.).K.'-(!1,)).+!A),(K*+(!-2,-!2,7.!-2.!,U*)*-=!

-&!0.A)*1,-.:!,'+!K,=!2,7.!-2.!,U*)*-=!-&!+*((.K*',-.!-2.K(.)7.(V!>2.!*((4.!&5!,'-*K*10&U*,)!0.(*(-,'1.!*(!

&5!E0,7.!1&'1.0'V!>2.!.K.0E.'1.!&5!K4)-*+04EQ0.(*(-,'-!Z9N/[!(-0,*'(!,'+!-2.!A&((*U*)*-=!&5!-0,'(5.0!&5!

-2*(! K4)-*+04E! 0.(*(-,'1.! -&! &-2.0! U,1-.0*,! 2,(! 0,*(.+! -2.! E0*K! (A.1-0.! &5! U,1-.0*,)! A,-2&E.'(! -2,-!

1,''&-!U.!1&'-0&)).+!U=!1400.'-)=!?'&X'!,'-*K*10&U*,)(:!,'+!-2.!0.Q.K.0E.'1.!&5!1400.'-)=!1&'-0&)),U).!

+*(.,(.(!-2,-!1,'!1,4(.!E)&U,)!A,'+.K*1(!&5!,A&1,)=A-*1!(1,).V!>2.!0*(.!*'!*'1*+.'1.!&5!9N/!U,1-.0*,!2,(!

U..'!,--0*U4-.+!-&!-2.!*'+*(10*K*',-.!4(.!&5!,'-*K*10&U*,)(!*'!,'*K,)!14)-40.!,'+!*'!K.+*1*'.V!W.0.!X.!

A0.(.'-! -2.! 54))!E.'&K.!(.C4.'1.(!&5! -X&!+04E! 0.(*(-,'1.!A),(K*+(! *(&),-.+! 50&K!5*(2!A,-2&E.'(!,'+!

+.(10*U.!-2.*0!U0&,+!*KA)*1,-*&'(!&'!-2.!.'7*0&'K.'-!,'+!*'!24K,'!2.,)-2V!!

%400.'-)=:!,!'.X!K4)-*+04E!0.(*(-,'1.!A),(K*+!A/$"S!X,(!*(&),-.+!50&K!!"#$%$&'()*+,#$-*./'!

&U-,*'.+!50&K!,!-*),A*,!Z0#"$1*#$%.()&./$2.13([!5,0K!*'!>2,*),'+V!A/$"S!*(!,!$R#:\TR!UA!1*014),0!A),(K*+!

1&'-,*'*'E!$"]!1&+*'E!0.E*&'(!.'1&+*'E!5&0!,!K.0140*1!0.(*(-,'1.!&A.0&':!E.'.(!5&0!-2.!>0,!1&'G4E,-*7.!

-0,'(5.0!K&+4).!,'+!-2.!+04E!0.(*(-,'1.!E.'.(!4'15:!6/'^_6Q$T:!'',!:!(3/7:!2"2!82"29:!,'+!1'2!:V!>2.)

A/$"SQ.'1&+.+! A0&-.*'(! Z'`$"][! X.0.! ,',)=Y.+! 4(*'E! -2.! K.-2&+! &5! A,*0QX*+.! 1&KA,0*(&'(! ,E,*'(-!

&-2.0!K&U*).!E.'.-*1!.).K.'-(!Z*'1)4+*'E!A),(K*+:!A2,E.!NJ6!,'+!(&!&'[!*'!-2.!6%P69a!Z,!1),((*5*1,-*&'!

&5!K&U*).!E.'.-*1!.).K.'-(!7.0(*&'!TV"[!+,-,U,(.V!A/$"S!(2&X.+!7.0=!2*E2!(*K*),0*-=!-&!AJN9Q$bN&?$:!

,'!9N/! A),(K*+! *(&),-.+! 50&K! ,! 24K,'! A,-2&E.'*1!;(1*"#.1*.') 1$/.:! ,'+! (2,0.+! 7.0=! 2*E2! 2&K&)&E=!

X*-2! &-2.0! 3'16c%! A),(K*+(! *(&),-.+! 50&K! 24K,':! ,'*K,):! ,'+! 5*(2! A,-2&E.'(:! *'1)4+*'E! ,! A0.7*&4()=!

*+.'-*5*.+! A),(K*+! AF\\QT$SV! 8*'1.! K&(-! &5! -2.! 2&(-(! &5! -2.! 3'16c%! A),(K*+(! X.0.! 24K,'! .'-.0*1!

U,1-.0*,!Z.


7 December 2012,


Tokyo, Japan !

! "#!

!"#"$%&'(%)"*+%$,'-.'/**01022,'(-345%#'!"#$%&''%()#&*+#,-.#+-/! %#!6-#78530'908":';50%30#2!

$%&'(!)'*%+%((%&%,-.&/!%&0!$%&('!1'&2%(

3!

/45+%.(65&(!-7!8%.'&5!$9'5&95:!;%95&(5.:!45+%.(65&(!-7!8%.'&5!%&0!>-%C(%=!I5C-

Book of Abstracts2012/12/04 · Erika Wahyu Dewanti, Kunihiko Futami, Takayuki Katagiri, Makoto...

Documents

Transcript of Book of Abstracts2012/12/04 · Erika Wahyu Dewanti, Kunihiko Futami, Takayuki Katagiri, Makoto...