Bone meta (-)Bone meta (+) n=101n=57p-value Age (min-max)73.0 (51-88)71.0 (55-86)0.027 BMI (min-max)...
8
Bone meta (-) Bone meta (+) n=101 n=57 p- value Age (min-max) 73.0 (51-88) 71.0 (55-86) 0.027 BMI (min-max) PrePSA (min-max) 24.0 (16.0-32) 20.0 (2.0- 982.0) 23.0 (17-28) 139.0 (7.8- 6262.0) 0.037 0.001 Gleason score 0.006 ≤6 24 (23.8) 3 (5.3) 7 29 (28.7) 15 (26.3) ≥8 48 (47.5) 39 (68.4) Posttreatment PSA nadir 0.04 (0.01- 30.4) 0.2 (0.01- 79.8) 0.04 A. C. B. T1 and T2 T3 and T4 Lee et al. Supp Fig 1
-
Upload
pastor-belles -
Category
Documents
-
view
216 -
download
0
Transcript of Bone meta (-)Bone meta (+) n=101n=57p-value Age (min-max)73.0 (51-88)71.0 (55-86)0.027 BMI (min-max)...
Bone meta (-) Bone meta (+)
n=101 n=57 p-value
Age (min-max) 73.0 (51-88) 71.0 (55-86) 0.027BMI (min-max)PrePSA (min-
max)
24.0 (16.0-32)20.0 (2.0-
982.0)
23.0 (17-28)139.0 (7.8-
6262.0)
0.0370.001
Gleason score 0.006≤6 24 (23.8) 3 (5.3)7 29 (28.7) 15 (26.3)
≥8 48 (47.5) 39 (68.4)Posttreatment
PSA nadir0.04 (0.01-
30.4)0.2 (0.01-79.8) 0.04
A.
C.
B.
T1 and T2 T3 and T4
Lee et al. Supp Fig 1
LNCaP 22RV1
Lee et al. Supp Fig 2
Lee et al. Supp Fig 3
A.*
0
50
100
150
200
250
Con BMP-6 1ng/ml BMP-610ng/ml
Cel
l co
un
ts (
% o
f co
n)
1d 2d 3d 4d
** *
**
*
B.
0
50
100
150
200
22RV1+WPMY-1 22RV1+HS-5
Ce
ll c
ou
nts
(%
of
co
n)
IgG BMP-6 Ab
C.
*
BMPRII
ActRIIa
ActRIIb
β-actin
Con
siRN
A
BM
PR
II siR
NA
ActR
IIa siR
NA
ActR
IIb siR
NA
BMPRIa
BMPRIb
ActRIa
β-actin
Con
siRN
A
BM
PR
Ia siR
NA
BM
PR
Ib siR
NA
ActR
Ia siR
NA
Smad1
Smad5
Smad8
β-actin
Con
siRN
A
Sm
ad1 siR
NA
Sm
ad5 siR
NA
Sm
ad8 siR
NA
Lee et al. Supp Fig 4
Wnt5a
β-actin
LNCaP 22RV1
WPM
Y-1
HS-5
WPM
Y-1
H-5Stromal
Cell line- - --
Lee et al. Supp Fig 5
Lee et al. Supp Fig 6
22RV1
SP600125:10uM, Calphostin C:100nM
Wnt5a - + + +
Inhibitor - -
SP60
012
5
Calp
hosti
n C
JNKi PKCi
0
1
2
3
4
5
6
7
8
9
Fo
ld in
cre
as
e
Wnt5a - + + +
Inhibitor - -
SP60
012
5
Calp
hosti
n C
JNKi PKCi
BMP-6
-actin
Lux
Lux
Lux
Lux
Lux
Lux
ATG
TATA CATNF-B
0 1 2 3 4
pGL3
0.3kb
0.6kb
0.9kb
1.2kb
1.5kb
BMP-6 Lux activity (Fold)
Lee et al. Supp Fig 7
PCKNF-B receptor
Lee et al. Supp Fig 8
