The simplest, quickest and most versatile Bioconjugation solution

Who are we?

Bioconjugation

What is nitzipper®?

How to use nitzipper®?

Why to use nitzipper®?

ApplicationsBecause your goal is the priority

Who are we?Here at nanoimmunotech, a European company in the Nanobiotechnology sector,

we are specialized and considered a benchmark in Bioconjugation.

The company has highly qualified and internationally recognized human resources,

state-of-the-art laboratory capabilities, standardized and proprietary protocols

and finally, the know-how to perform proper supervision, advising and validation

while creating different nanosystems or biomaterials; first step to their previous use

in biotechnological applications for different sectors (biomedical, pharmaceutics,

cosmetics, veterinary, agro-food, environmental, etc.).

At nanoimmunotech, we believe in creative researchers who want to explore new

territories. We believe in those who do not like to be told how to do something nor

be restricted or limited in capabilities. We believe in those whose goal is not just

having tools but needing those tools to achieve their goals.

This is why, for all of you independent researchers with scientific curiosities, we

have created an innovative technology called nitzipper®. The simplest, quickest

and most versatile bioconjugation technology that makes it possible for you to

join many of the molecules, biomolecules, particles, linkers, drugs, etc.You need

to create the most inspiring materials or applications.

because your goal is the priority, not the tool you use to achieve it.

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Because your goal is the priority, not the tool you use to achieve it.

BioconjugationNowadays, the main problem regarding bioconjugation is that the conjugation

processes are different, depending on the variety of micro/nanostructures or

biomolecules used; mainly due to the wide gap existing between them in terms

of size, surface area, density of reactive groups, shape, colloidal stability, etc.

Moreover, when designing a material bioconjugation protocol, it is important to

respect the orientation of biomolecules on the surface of the material in order to

maintain its biological activity, and therefore its efficiency.

Currently, there is no universal tool that would easily enable conjugation of similar

particles or bio/molecules with one another while ensuring oriented unions,

much less the ability to easily conjugate different kinds of molecules with each

other. Consequently, customers require tailor-made projects in order to optimize

complex binding protocols. With experienced personnel, projects are time

consuming and expensive solutions completed through inefficient bioconjugation

methods. The other option is not any better: customers need to purchase specific,

individual kits for the conjugation of specific molecules and particles, for their

use in specific applications, such as magnetic separation, antibody labeling, etc.,

without ensuring obtaining good results.

With nitzipper®, the new innovative technology of nanoimmunotech, we offer you the almost universal, economical and single-step bioconjugation solution which allows mono- and multi-conjugation of most micro/nanostructures, biomolecules, linkers, drugs and other molecules, creating powerful micro/nanosystems, resulting in the sum of the functions for each of its components.

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What is nitzipper®?nitzipper® is a new and revolutionary family of products ready to be used in an

easy, immediate and effective conjugation through an innovative bioconjugation

technology based on the complementarity of the Linker U and Linker T, that allows

you to combine many different types of micro/nanostructures, biomolecules

(antibodies, dyes, enzymes, peptides and other molecules), drugs, etc., in

practically any combination, in an oriented way and without modifying their

properties.

NITZIPPER® added valueIts great VERSATILITY that opens infinite doors to the bioconjugation world.

With nitzipper® you can also perform multi-functionalization, joining not only

two different types of molecules or micro/nanostructures with each other, but

many of them simultaneously, making possible to create a multitude of new and

sophisticated conjugates for their use in infinite applications.

It is not a specific solution for a specific application. The nitzipper® products

can be used to improve traditional applications (antibody labeling, immunoassays,

flow cytometry, ELISA, biosensing, magnetic separation, and many others) but

also to explore even the most unthinkable new applications.

You already have developed your own biomolecule or micro/nanostructure and you want to conju-gate it? We can make it compatible with the nitzipper® technology and so, you will be able to use it with any other nitzipper® product.

Versatility, regarding all the products combinations you can design

A strong and stable union between the different pieces of the micro/nanosys-tems is performed thanks to matching linkers named Linker U and Linker T, each one on the surface of nitzipper® pieces.

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Once you have received the nitzipper®

products, simply mix the appropriate

quantity of piece with Linker U you wish to

connect with the piece with Linker T and wait

a few minutes to quickly achieve a stable

conjugation between both pieces.

nitzipper® is compatible with many more structures and molecules than the ones you see in the store. If you cannot find the pieces you need or if you want us to make a custom piece, specific for your micro/nanostructures and/or molecules, compatible with our technology, just contact us:

customer_support@nanoimmunotech.es

How to use nitzipper®?A SINGLE BIOCONJUGATION STEP

You now have your conjugateready to use!

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Select and purchase the products/pieces that you wish to conjugate

in our nitzipper® store. As an example, you can choose to purchase

a magnetic nanoparticle with Linker U (yellow tape tube) and an

antibody with Linker T (red tape tube), or an antibody with Linker U

and a dye with Linker T or… The possibilities are endless.

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Let’s focus on what really matters. Whether you have the

knowledge on conjugation techniques or not, in just 20-

30 seconds you will have performed all the manipulations

required to conjugate different compounds.

Saves Time

Simplicity

Why to use nitzipper®?Versatility

Avoids searching for different solutions for every

type of conjugation and helps reduce the stock of

products you need.You can bioconjugate any kind of

nitzipper® piece with any other. You simply need to choose

pieces with the matching Linker U and Linker T.

Biomolecules are conjugated in a properly oriented direction

avoiding, for example, blocking the antibody recognition

region. As a result, you receive better results for your

application.

Better Results

nitzipper® is a strong and stable union that binds in an

oriented way and without altering the properties of the

micro/nanostructures or molecules.

Stable

Strong and stable bonds. nitzipper® can be used in many

applications with the certainty that it will work.

Efficiency

High-standard results can be obtained with less quantity of

biomolecules or particles, which significantly reduces your

conjugation costs.

Reduce costs=+

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With a single step of mixing the pieces of Linker U with the

pieces of Linker T, you will be able to carry out the

bioconjugation of your micro/nanostructures and/or

molecules, without being an expert or needing any additional

equipment.

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“ nitzipper® is the simplest, quickestand most versatile bioconjugationsolution”

Efficiency Medium Medium Medium

Parameters nitzipper® Biotinylation kit Other Bioconjugation kits (propietary linker technology)Amine reactive linker

Operator’s Manipulation Time

Preactivation step

Bioconjugation Overall Time

Desalting and/or purification step

Application Versatility

Operator’s Level of Qualification

Average Cost

Efficiency

Incompatibilities

Technologies

High Medium Medium

1h to 24h

30min 30min

30min to 3h

20-30sec up to 1h

30min to 24h

required required coul be required

15min

20-30sec

not required

not required

not required could be required could be required

Medium

High

No

Medium

Medium

Medium

may vary

Low Medium

Medium

Medium

Amine cointaining

buffers

Background in application

due to endogen biotin

Medium-High

Medium Medium

Medium

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Biosensing

Versatility that opens new doors to infinite applications

Flow cytometryMagnetic Separation

Nanovaccines

Immuno-PCR

Antibody Labelling

Immunofluorescence

Immunoassays

ELISA

Magnetic hyperthermia

Develop your own application with nitzipper bioconjugation technology

ApplicationsThousands of scientists, both in academia and industry, use bioconjugation in

multiple applications.

Thanks to its incredible versatility, nitzipper® bioconjugation technology offers

an endless panel of applications, performing a variety of different combinations

with varied micro/nanostructures and/or biomolecules within different

applications. Additionally, each combination can be utilized for different kinds of

applications.

Here you can find some examples of applications that you can develop with

nitzipper®. Some of them were performed by nanoimmunotech while others

are results our customers achieved through different uses and applications in

many different sectors such as: biotechnology, pharmaceutics, cosmetics, agro

food, environment, veterinary and many others. With or without experience in

bioconjugation, our customers achieve successful results, thanks to nitzipper®

technology.

So focus on your own application and be the next to use nitzipper®. Remember,

nitzipper® applications are infinite, only limited by your own imagination.

Should you need assistance or advice when planning or designing your new

application, just let us know and we will be glad to help you:

customer_support@nanoimmunotech.es

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HEADQUARTERS IN ZARAGOZA, SPAINEdificio Cero Emisiones Avda. Autonomía 7 0003 Zaragoza, Spain

Phone: (+34) 976 369 300

HEADQUARTERS IN VIGO, SPAINEdificio CITEXVI Fonte das Abelleiras s/nCampus Universitario de Vigo36310 Vigo, Pontevedra, Spain

Phone: (+34) 986 167 990

info@nanoimmunotech.es

www.nitzipper.com

Your Bioconjugation Friendly Solution