Blocking C-Raf alleviated high-dose small-volume …10.1038...Blocking C-Raf alleviated high-dose...
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Blocking C-Raf alleviated high-dose small-volume radiation-induced epithelial mesenchymal transition in mice lung Zhen-Yu Hong a,b# , Sanke Li c# , Xiaomei Liu a , Xiao-Min Leng d , Zhanhui Miao a , Xiaohong Kang a , Hongrui Niu a , Ming-Qing Gao e* , Ping Lu a* a Department of Medical Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China. b Department of Radiation Oncology, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, South Korea. c Department of Hematology, the 3rd Peo ple’s Hospital of Zhengzhou, China. d Henan Key Laboratory of Neural Regeneration, Department of Neurology, The First Affiliated Hospital of Xinxia ng Medical University, Weihui, Henan, China. e School of Medicine, Northwest University, Shaanxi, China # Zhen-Yu Hong and San-Ke Li contributed equally to the manuscript and share first authorship *Reprint requests to: 1. Ping Lu, MD, PhD. Professor and Head Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, 88 Jiangkang Road, Weihui, Henan, P.R. China Zip 453100， Office) +86-373-440-2543 Mobile) +86-135-9872-2864 E-mail) [email protected] & 2. Ming-Qing Gao, PhD Professor School of Medicine, Northwest University. Taibai North Road 229, Xi’an 710069, Shaanxi, P.R. China E-mail) [email protected]
Transcript of Blocking C-Raf alleviated high-dose small-volume …10.1038...Blocking C-Raf alleviated high-dose...
Blocking C-Raf alleviated high-dose small-volume radiation-induced epithelial mesenchymal transition in mice lung
Zhen-Yu Honga,b#, Sanke Lic#, Xiaomei Liua, Xiao-Min Lengd, Zhanhui Miaoa, Xiaohong Kanga, Hongrui Niua, Ming-Qing Gaoe*, Ping Lua*
a Department of Medical Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China. b Department of Radiation
Oncology, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, South Korea. c Department of Hematology, the 3rd Peo
ple’s Hospital of Zhengzhou, China. d Henan Key Laboratory of Neural Regeneration, Department of Neurology, The First Affiliated Hospital of Xinxia
ng Medical University, Weihui, Henan, China. e School of Medicine, Northwest University, Shaanxi, China
# Zhen-Yu Hong and San-Ke Li contributed equally to the manuscript and share first authorship
*Reprint requests to:
1. Ping Lu, MD, PhD.
Professor and Head
Department of Oncology,
The First Affiliated Hospital of Xinxiang Medical University,
88 Jiangkang Road, Weihui, Henan, P.R. China
Zip 453100， Office) +86-373-440-2543
Mobile) +86-135-9872-2864 E-mail) [email protected]
&
2. Ming-Qing Gao, PhD
Professor
School of Medicine, Northwest University.
Taibai North Road 229, Xi’an 710069, Shaanxi, P.R. China
E-mail) [email protected]
Supplementary Figure S1. Full unedited gel for Figure 2 A
Control 70Gy Xray
2W 4W 2W 4W
p-C-Raf C-Raf
Control 70Gy Xray
2W 4W 2W 4W
Supplementary Figure S2. Full unedited gel for E-cadherin in Figure 3 B, and repeated experiment with different exposure time
24h 48h 72h
8 Gy
control
repeated experiment with different exposure time
Blots in main Figure 3B Original Blots of E-cadherin
Supplementary Figure S3. Full unedited gel for α-SMA in Figure 3 B, and repeated experiment with different exposure time
24h 48h 72h control
8 Gy
Original Blots of α-SMA
Blots in main Figure 3B
24h 48h 72h control
8 Gy
24h 48h 72h control
8 Gy
Supplementary Figure S4. Full unedited gel for GAPDH in Figure 3 B, and repeated experiment with different exposure time
Original Blots of GAPDH
repeated experiments with different exposure time
Blots in main Figure 3B
Supplementary Figure S5. Full unedited gel for p-C-Raf in Figure 4 A
p-C-Raf
Original Blots of p-C-Raf
Blots in main Figure 4A
Supplementary Figure S6. Full unedited gel C-raf In for Figure 4 A
C-raf
Original Blots of C-Raf
Blots in main Figure 4A
Supplementary Figure S7. Full unedited gel for GAPDH in Figure 4 A
Original Blots of GAPDH
Blots in main Figure 4A
Supplementary Figure S8. Full unedited gel for p-C-Raf in Figure 4 B
p-C-Raf
Original Blots of p-C-Raf
Blots in main Figure 4B
Supplementary Figure S9. Full unedited gel for GAPDH in Figure 4 B
GAPDH
Original Blots of GAPDH
Blots in main Figure 4B
Supplementary Figure S10. Full unedited gel for E-cadherin in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of E-cadherin
Blots in main Figure 4C
Supplementary Figure S11. Full unedited gel for α-SMA in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of α-SMA
Blots in main Figure 4C
Supplementary Figure S12. Full unedited gel for GAPDH in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of GAPDH
Blots in main Figure 4C
Supplementary Figure S13. Full unedited gel for Twist1 in Figure 5 A, and repeated experiment with different exposure time
repeated experiments with
different exposure time
Blots in main Figure 5A
Original Blots of Twist1
Supplementary Figure S14. Full unedited gel for GAPDH in Figure 5 A, and repeated experiment with different exposure time
Blots in main Figure 5A
Original Blots of GAPDH
Supplementary Figure S15. Full unedited gel for α-SMA in Figure 5 B, and repeated experiment with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Original Blots of α-SMA
repeated experiments with different exposure time
Blots in main Figure 5B
Supplementary Figure S16. Full unedited gel for GAPDH in Figure 5 B, and repeated experiment with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Blots in main Figure 5B Original Blots of GAPDH
repeated experiments with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S17. Full unedited gel for p-C-Raf in Figure 5 B, and repeated experiment with different exposure time
Original Blots of p-C-Raf Blots in main Figure 5B
repeated experiments with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S18. Full unedited gel for E-cadherin in Figure 5 B, and repeated experiment with different exposure time
repeated experiments with different exposure time
Original Blots of E-cadherin Blots in main Figure 5B
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S19. Full unedited gel for Twisr1 in Figure 5 B, and repeated experiment with different exposure time
Original Blots of Twist1
Blots in main Figure 5B
repeated experiments with different exposure time
