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Transcript of Biotechnology Questions
Biotechnology Questions, Paper - 01
Questions Answers
1. The term cistorn, muton and recon were introduced by(A) Watson and Crick(B) S. Benzer(C) Meselson(D) Morgan
Answer: (B)
2. Extranuclear genetic material is found in (A) Plastid and nucleus(B) Mitochondria and plastids(C) Nucleus and cytoplasm(D) Mitochondria and nucleus
Answer: (B)
3. The molecular formulae of deoxyribose sugar and ribose sugar respectively are(A) C5 H10 O4 and C5H10O6 (B) C5 H10 O4 and C5H10O5 (C) C5 H10 O5 and C5H10O4 (D) C5 H10 O5 and C6H10O4
Answer: (B)
4. The nitrogen bases which pair with two hydrogen bonds are(A) Adenine and thymine(B) Adenine and Cytosine(C) Cytosine and guanine(D) Cytosine and adenine
Answer: (A)
5. DNA differs from RNA in(A) Presence of deoxyribose sugar(B) Presence of thymine base(C) Property of replication(D) All the above
Answer: (D)
6. DNA molecules makes a complete turn after every(A) 20 Å(B) 34 Å(B) 3.4 Å(D) 10 base pairs
Answer: (D)
7. The distance between two successive nitrogenous base pairs is(A) 34 Å(B) 36 Å (C) 20 Å(D) 3.4 Å
Answer: (D)
8. In nucleoside, nitrogen base is attached to pentose sugar at(A) Carbon – 1 of pentose sugar(B) Carbon – 2 of pentose sugar(C) Carbon – 4 of pentose sugar(D) Carbon – 5 of pentose sugar
Answer: (A)
9. If the strand of DNA has 35 nucleotide how many phosphodiester bonds would exist(A) 34(B) 35(C) 24(D) 70
Answer: (A)
10. In eukaryotic DNA replication, lagging strand is formed by(A) RNA fragments(B) Okazaki fragments(C) DNA fragments (D) Nucleotide fragments
Answer: (B)
11. The enzyme DNA polymerase can work only in(A) 3 5 direction(B) 5 3 direction(C) Both the direction(D) 5 5 direction
Answer: (B)
12. Enzyme required for removing RNA primer during DNA replication is(A) DNA primase (B) DNA ligase(C) DNA polymerase I(D) DNA polymerase III
Answer: (C)
13. During DNA replication, the reunion or recoiling of separated DNA strand is prevented by(A) Helix destabilizing protein(B) Single strnad binding protein(C) Rep protein(D) Both (A) and (B)
Answer: (D)
14. The enzyme that cuts the bonds of DNA molecule at the origin of replication is(A) Endonuclease(B) DNA polymerase(C) DNA gyrase(D) DNA ligase
Answer: (A)
15. Which of the following enzyme is required to release the tension imposed by uncoiling of strands?(A) Endonuclease(B) DNA ligase(C) DNA gyrase(D) DNA helicase
Answer: (C)
16. The cellular composition of m-RNA is(A) 5-10%(B) 3-5%(C) 10-20 %(D) 70-80%
Answer: (B)
17. Formation of mRNA from DNA is called(A) Transformation(B) Transduction(C) Traslation(D) Transcription
Answer: (D)
18. The ratio of purines and pyrimidines in mRNA is not 1:1 because the nitrogenous bases are(A) Unpaired(B) Paired(C) Paired only in loops(D) Paired in stems
Answer: (A)
19. The codons which may present at 3¢ end of mRNA(A) UAA(B) UAG(C) UGA(D) Any one of these
Answer: (D)
20. Which of the following is not tool of genetic engineering?(A) Vectors(B) Enzymes(C) Foreign DNA(D) GMO
Answer: (D)
21. In recombinant DNA technology a plasmid vector is cleaved by(A) Modified DNA ligase(B) A heated alkaline solution(C) The same enzyme that cleave the donor DNA
Answer: (C)
(D) The different enzyme other than that cleave the donor DNA
22. The most common plasmid vector used in genetic engineering is(A) PBR 328(B) PBR 322(C) PBR 325(D) PBR 330
Answer: (B)
23. ‘Nif gene’ for nitrogen fixation is cereal crops like wheat, jowar etc. is introduced by cloning(A) Rhizobium meliloti(B) Bacillus thuringiensis(C) Rhizopus(D) Rhizophora
Answer: (A)
24. Eco RI is an(A) Ligase(B) Polymerase(C) Restriction enzyme(D) Gyrase
Answer: (C)
25. The transgenic plant flavr savr tomato carries an artificial gene for(A) Delay ripening process(B) Longer shell life(C) Added flavours(D) All of these
Answer: (D)
Biotechnology Questions, Paper - 02
Questions Answers
1. Hirudin is obtained from the transgenic plant(A) Brassica napus(B) Hibiscus rosasinesis(C) Raphanus sativus(D) Vinca rosea
Answer: (A)
2. Bt Cotton is(A) Cloned plant(B) Transgenic plant(C) Hybrid plant(D) Mutated plant
Answer: (B)
3. Dolly sheep was genetically similar to (A) The mother from which nucleated fertilized egg was taken(B) The mother from which nuclear DNA of udder cell was taken(C) The surrogate mother(D) Both surrogate mother and nuclear donor mother
Answer: (B)
4. Genome is(A) Genes on nuclear DNA(B) Nuclear DNA + mitochondrial DNA(C) Nuclear DNA + chloroplast DNA(D) Nuclear DNA + Mitochondrial DNA + Chloroplast DNA
Answer: (D)
5. A technique of using very small metal particles coated with desired gene in the gene transfer is called(A) Electroporation(B) Microinjection(C) Liposome(D) Biolistics
Answer: (D)
6. The complete set of chromosomal and extrachromosomal genes of an organisms is called(A) Genome(B) Gene pool(C) Gene bank(D) Gene library
Answer: (A)
7. The study of all the proteins coded by the genome is called(A) Proteome(B) Proteomics(C) Genome(D) Protein formation
Answer: (B)
8. Sequencing of genomic DNA is included under(A) Structural genomics(B) Functional genomics(C) Proteomics(D) Transgenesis
Answer: (A)
9. Gene expression, regulation and phenotype production are studied in second phase of genome analysis called(A) Structural genomics(B) Functional genomics(C) Proteomics(D) Transmeiosis
Answer: (B)
10. A flowering plant lily have ______ more DNA than humans(A) 10 times(B) 15 times(C) 18 times(D) 13 times
Answer: (C)
11. In forensic science which of the following is used?(A) Bacterial cloning(B) DNA foot printing(C) DNA fingerprinting(D) DNA cloning
Answer: (C)
12. DNA fingerprinting is based on(A) Occurance of VNTR’s (B) Knowledge of human karyotype(C) Cloned DNA(D) Recombinant DNA
Answer: (A)
13. VNTRs represnets-(A) New terminal regions in DNA(B) Functional genes in the DNA(C) Split genes in the sample DNA(D) Specific non-coding sequences with unique tandem repeats
Answer: (D)
14. Which ones produce androgenic haploids in anther cultures?(A) Anther wall(B) Tapetal layer of anther wall(C) Connective tissue(D) Young pollengrains
Answer: (D)
15. Variations observed during tissue culture of some plants are known as(A) Clonal variations(B) Somatic variations(C) Somaclonal variations(D) Tissue culture variations
Answer: (C)
16. Virus free plants can be obtained through(A) Anitibiotic treatment(B) Bordeaux micture
Answer: (D)
(C) Root tip culture(D) Shoot tip culture
17. To raising of plants from a small tissue in culture is known as(A) Macroproduction(B) Micropropagation(C) Tissue culture(D) Mass production
Answer: (B)
18. Callus is(A) Tissue that forms embryo(B) an insoluble carbohydrate(C) Unorganised actively dividing mass of cells maintained in culture(D) Tissue that growth to form embryoid
Answer: (C)
19. Biopatents are ______.(A) Right to use invention(B) Right to use biological entities(C) Right to use products(D) Right to use process
Answer: (B)
20. African plant Pentadiplandra is used as______.(A) Low calories sweetner(B) 2000 times sweeter agent(C) Sweetner for diabetic patients(D) All of these
Answer: (D)
21. Which organism was used as bioweapon derived from______.(A) Clostridium(B) Yerstsinia pestis(C) Fusarium species(D) Green algae
Answer: (C)
22. A set standards used to regulate own or community activity in relation to biological world is(A) Biopotency(B) Biopiracy(C) Biowar(D) Bioethics
Answer: (D)
23. Biopiracy means(A) Use of biopatents(B) Thefts of plants and animals(C) Stealing of bioresources(D) Exploitation of bioresources without authentic permission
Answer: (D)
24. Bioethcs is related to(A) Preventing biopiracy(B) Regulation of unethical activities likegene cloning in animals(C) Preventing theft of living materials(D) Moral guidance to the problems in biology
Answer: (B)
25. Three dimensional shape of tRNA is(A) L-shaped(B) Clover leaf-like(C) X-shaped(D) Y-shaped
Answer: (B)
Biotechnology Questions, Paper - 03
Sr. No. Questions Answers
1. A DNA nucleotide chain has AGCTTCGA sequence of other chain would be(a) TCGAAGCT(b) GCTAAGCT(c) TAGCATAT(d) GATCCTAG
Answer: (a)
2. A nucleoside is formed of (a) Pentose sugar, phosphate and nitrogen base(b) phosphate and nitrogen base(c) Pentose sugar and phosphate(d) Pentose sugar and nitrogen base
Answer: (d)
3. A nucleotide is formed of (a) Purine, Pyrimidine and phosphate(b) Purine, Sugar and phosphate(c) Nitrogen base, Sugar and phosphate(d) Pyrimidine, Sugar and phosphate
Answer: (c)
4. A riboside is(a) Base + phosphate(b) Ribose + phosphate(c) Ribose + phosphate + base(d) Ribose + base
Answer: (d)
5. A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is(a) 120(b) 240(c) 60(d) 480
Answer: (d)
6. A Strand of DNA has base sequence CATGACTAG. The base sequence on the other strand would be(a) CAT TAG GAC(b) GTA CTG ATC(c) GAT GTC ATC(d) TAC ACT GCT
Answer: (b)
7. A totipotent cell means(a) An undifferentiated cell capable of developing into a system or entire plant(b) An undifferentiated cell capable of developing into an organ(c) An undifferentiated cell capable of developing into complete embryo(d) Cell which lacks the capability differentiate into an organ or system
Answer: (a)
8. Adenine is(a) Purine(b) Pyrimidine(c) Nucleoside(d) Nucleotide
Answer: (a)
9. Amino acid binding site of tRNA is(a) 5’end(b) Anticodon loop(c) DHU loop(d) -CCA 3’end
Answer: (d)
10. Anticodon occurs in(a) tRNA(b) mRNA(c) mtRNA
Answer: (a)
(d) rRNA
11. Bacterial plasmid contains(a) RNA(b) RNA + protein(c) DNA(d) Photosynthetic structures
Answer: (c)
12. Base pairs present in one turn of DNA are (a) 12(b) 11(c) 10(d) 9
Answer: (c)
13. Best method to determine paternity is (a) Protein analysis(b) Chromosome counting(c) Gene counting(d) DNA finger printing
Answer: (d)
14. Callus is (a) Tissue that forms embryo (b) An insoluble carbohdrate(c) Tissue that grows to form embryoid(d) Unorganised actively dividing mass of cells maintained in culture
Answer: (d)
15. Chemical Knives/ molecular scissors of DNA are(a) Restriction endonucleases(b) Polymerases(c) Ligases(d)Transcriptases
Answer: (a)
16. Chemofusion and electrofusion are employed in(a) Eugenics(b) Protoplast fusion(c) Cloning(d) Mutations
Answer: (b)
17. Choose the correct statement (a) DNA is hereditary material(b) RNA is hereditary material(c) DNA is hereditary material but where it is absent RNA can function as hereditary material(d) Both DNA and RNA are hereditary materials
Answer: (c)
18. Development of shoot and root in tissue culture is determined by(a) Cytokinin and auxin ratio(b) Enzymes(c) Temperature(d) Plant nutrients
Answer: (a)
19. Distance between two base pairs of DNA is(a) 34 nm(b) 3.4 nm(c) 0.68 nm(d) 0.34 nm
Answer: (d)
20. Distance between two strands of DNA is(a) 34 Å (b) 20 Å (c) 3.4 Å (d) 340 Å
Answer: (b)
Biotechnology Questions, Paper - 04
Sr. No. Questions Answers
1. Distance between two successive nitrogenous bases or base pairs of DNA is (a) 34 Å (b) 3.4 Å (c) 10 Å (d) 5 Å
Answer: (b)
2. DNA and RNA are similar in having(a) Similar nucleotides(b) Similar pyrimidines(c) Similar purines(d) Similar sugars
Answer: (c)
3. DNA and RNA show similarity in having(a) Polymers of nucleotides(b) Similar pyrimidines(c) Double strands(d) Similar sugars
Answer: (a)
4. DNA does not occur in(a) Nucleus (b) Ribosomes(c) Mitochondria(d) Plastids
Answer: (b)
5. DNA duplex shows (a) Right handed coiling and parallel(b) Right handed coiling and antiparallel(c) Left handed coiling and antiparallel(d) Left handed coiling and parallel
Answer: (b)
6. DNA is composed of repeating units of(a) Ribonucleosides(b) Deoxyribonucleosides(c) Ribonucleotides(d) Deoxyribonucleotides
Answer: (d)
7. DNA replication in eucaryotes commences(a) From both ends of a chromosome simultaneously (b) Several sites along DNA of a chromosome simultaneously(c) From centromere to either end(d) From one end of chromosome to the other
Answer: (b)
8. DNA replication is(a) Conservative and discontinuous(b) Semiconservative and semidiscontinuous(c) Semiconservative and discontinuous(d) Conservative
Answer: (c)
9. DNA resembles RNA as both have(a) Polymers of nucleotides(b) Similar sugars(c) Similar pyrimidine bases(d) Ability to replicate
Answer: (a)
10. DNA sequence is ATG. What would be the sequence of bases in anticodon of tRNA(a) ATG
Answer: (b)
(b) AUG(c) UAC(d) TAC
11. DNA sequence is TAG. What shall be the sequence in anticodon of tRNA(a) UAG(b) ATC(c) ATG(d) UAC
Answer: (a)
12. DNA sequence of ATTCGATG is transcribed as(a) AUUCGAUG(b) UAAGCUAC(c) CAUCGAAU(d) GUAGCUUA
Answer: (b)
13. DNA strand with nitrogen base sequence ATTGCC will have sequence in mRNA(a) UAACGC(b) ATCGCC(c) ATTGCA(d) AGGACC
Answer: (a)
14. Double chained DNA strand is made radioactive in both its chains. It is allowed to replicate twice in non-radioactive medium. The result would be(a) All strands have radioactivity(b) Half the strands have radioactivity(c) Three strands have radioactivity(d) Radioactivity is absent in all strands
Answer: (b)
15. Double helical structure of DNA was proposed by(a) Kornberg(b) Nirenberg(c) Watson and Crick(d) Wilkins and Franklin
Answer: (c)
16. Functional unit of gene that specifies synthesis of one polypeptide is(a) Codon(b) Cistron(c) Recon(d) Muton
Answer: (b)
17. Gene is segment of (a) RNA(b) DNA(c) RNA or DNA(d) Both DNA and RNA
Answer: (b)
18. Genetically engineered bacteria are being used in commercial production of(a) Melatonin(b) Testoteron (c) Human insuline(d) Thyroxine
Answer: (c)
19. Haploid plant cultures are got from(a) Leaves(b) Root tip(c) Pollen grain(d) Buds
Answer: (c)
20. Hydrogen bonds present between cytosine and guanosine are(a) 2(b) 3(c) 1
Answer: (b)
(d) 4
Biotechnology Questions, Paper - 05
Sr. No. Questions Answers
1. In a DNA molecule cytosine is 18%. Percentage of adenine would be(a) 32%(b) 64%(c) 36%(d) 18%
Answer: (a)
2. In AGCT of DNA hydrogen bonds and base pairings occur between(a) A-U, C-G(b) A-C, G-T(c) A-G,C-T(d) A-T,C-G
Answer: (d)
3. In callus culture, roots can be induced by the supply of (a) Auxin and no cytokinin(b) Higher concentration of auxin and lower concentration of cytokinin(c) Higher concentration of cytokinin and lower concentration of auxin(d) Both auxin and cytokinin in equal proportions.
Answer: (b)
4. In DNA, adenine pairs with(a) Guanine(b) Thymine(c) Cytosine(d) Uracil
Answer: (b)
5. In DNA, guanine lies opposite(a) Uracil(b) Cytosine(c) Adenine(d) thymine
Answer: (b)
6. In double helix of DNA, the two DNA strands are(a) Coiled around a common axis(b) coiled around each other(c) coiled differently(d) Colied over protein sheath
Answer: (a)
7. In RNA, thymine is replaced by(a) Adenine(b) Guanine(c) Cytosine(d) Uracil
Answer: (d)
8. In tissue / bacterial culture glassware and nutrients are sterilized through(a) Water bath at 200° C(b) Dry air oven at 200° C(c) Dehumidifire(d) Autoclave
Answer: (d)
9. In tissue culture, callus can be induced to form shoot or root by altering the ratio of(a) Auxin to cytokinin(b) Cytokinin to ethylene
Answer: (a)
(c) Auxin to gibberellin(d) Gibberellin to cytokinin
10. Initiation codon of protein synthesis (in eucaryotes) is(a) GUA(b) GCA(c) CCA(d) AUG
Answer: (d)
11. Introduction of foreign genes for improving genotype is(a) Tissue culture(b) Immunisation(c) Biotechnology(d) Genetic engineering
Answer: (b)
12. It is now possible to breed plants and animals with desired characters through(a) Genetic engineering(b) Chromosome engineering(c) Ikebana technique(d) Bonsia technique
Answer: (a)
13. Most abundant RNA of the cell of(a) tRNA(b) rRNA(c) mRNA(d) tRNA
Answer: (b)
14. Nitrogen bases of DNA are (a) ATUC(b) UTGC(c) ATGC(d) AUGC
Answer: (c)
15. Nonsense codon takes part in(a) Terminating message of gene controlled protein synthesis(b) Formation of unspecified amino acids(c) Conversion of sense DNA into non-sense one(d) Releasing tRNA from polypeptide chain
Answer: (a)
16. Nucleosides are(a) Sugar + Phosphoric acid(b) Purine / Pyrimidine + Sugar + Phosphate(c) Purine / Pyrimidine + Phosphoric acid(d) Purine / Pyrimidine + Sugar
Answer: (d)
17. Nucleotides present in one turn of DNA helix (a) 4(b) 8(c) 10(d) 9
Answer: (c)
18. Okazaki segments are formed during(a) Transduction(b) Transcription(c) Replication(d) Translation
Answer: (c)
19. Plants developed in vitro culture from pollen grains are(a) Androgenic plants(b) Pollen plants(c) Male plants(d) Sterile plants
Answer: (a)
20. Plasmids are vectors for gene cloning because they Answer: (a)
(a) Self replicate in bacterial cells(b) Replicate freely outside bacterial cells(c) Can be multiplied in culture(d) Can be multiplied in laboratories using enzymes
Biotechnology Questions, Paper - 06
Sr. No. Questions Answers
1. Pomato is somatic(a) Poppy and Potato(b) Potato and tomato(c) Poppy and tamarind(d) Poppy and Tomato
Answer: (b)
2. Preserving germplasm in frozen state is(a) Cryopreservation(b) Cold storage(c) In situ preservation(d) Vernalisation
Answer: (a)
3. Protein helping in opening of DNA double helix in front of replication fork is(a) DNA gyrase(b) DNA Polymerase I(c) DNA ligase(d) DNA topoisomerase
Answer: (a)
4. Pyrimidine base present in RNA in place of thymine of DNA is(a) Uracil(b) Adenine(c) Cytosine(d) Guanine
Answer: (a)
5. Restriction endonuclease is employed for cutting(a) A single stranded DNA(b) Double stranded DNA(c) RNA fragment(d) mRNA
Answer: (b)
6. Restriction endonucleases are(a) Used in genetic engineering for uniting two DNA molecules(b) Used for in vitro DNA synthesis(c) Present in mammalian cells for degeneration of DNA of dead cells(d) Synthesised by bacteria for their defence
Answer: (d)
7. Restriction enzymes are used in genetic engineering because they(a) Can join DNA fragment (b) Cut DNA at specific base sequence(c) Cut DNA at variable sites(d) Are proteolytic enzymes which degrade harmful proteins
Answer: (d)
8. Reverse transcriptase is(a) RNA dependent RNA polymerase
Answer: (d)
(b) DNA dependent RNA polymerase(c) DNA dependent DNA polymerase(d) RNA dependent DNA polymerase
9. RNA contains(a) Hexose(b) Ribose(c) Fructose(d) Glucose
Answer: (b)
10. RNA does not possess(a) Uracil(b) Thymine(c) Adenine(d) Cytosine
Answer: (b)
11. RNA that picks up specific amino acid from amino acid pool of cytoplasm to carry it to ribosome during protein synthesis is(a) tRNA(b) mRNA(c) rRNA(d) gRNA
Answer: (a)
12. Semiconservative DNA / Chromosome replication using 14N was demonstrated by(a) Messelson(b) Tylor(c) Messelson and stahl(d) Hershey and Chase
Answer: (c)
13. Similarity between DNA and RNA is that both have(a) Similar sugars(b) Similar mode of replication(c) Similar pyrimidines(d) Polymers of nucleotides
Answer: (d)
14. Structure of DNA was given by(a) Kornberg(b) Nirenberg(c) Watson and Crick(d) Holley and Nirenberg
Answer: (c)
15. Successive nucleotides are covalently linked through(a) Glycosidic bonds(b) Phosphodiester bonds(c) Hydrogen bonds(d) Nitrogen bonds
Answer: (b)
16. Termination of polypeptide chain is brought about by(a) UUG, UAG and UCG(b) UAA, UAG and UGA(c) UUG, UGC and UCA(d) UCG, GCG and ACC
Answer: (b)
17. The basic uniot of a nucleic acid is(a) Pentose sugar(b) Nucleoid(c) Nucleoside(d) Nucleotide
Answer: (d)
18. The codon for anticodon 3’ UUUA - 5’ is(a) 5’ AAAU- 3’(b) 5’ UUUA- 3’(c) 3’ UAAD- 5’
Answer: (a)
(d) 3’ AUUU- 5’
19. The common feature amongst nucleus, chloroplast and mitochondria is(a) Lamellae(b) DNA(c) Cristae(d) All the above
Answer: (b)
20. The enzyme taking part in joining two ends of DNA is(a) Ligase(b) Polymerase(c) Gyrase(d) Helicase
Answer: (a)
CHAPTER – 9
1. Genetic engineering is the manipulation of ________ for practical purpose.
Genetic bacteria
Genetic plant
Genetic material
Genetic animal
2. The ________ technology has launched an industrial revolution in bio-technology.
DNA transfer
DNA recombinant
genetic bacteria
genetic transferring
3. Recent reproductive cloning intended to introduce ________.
identical plants
genetic bacteria
identical animals
identical sheeps
4. ________ was the first ever cloned animal.
Dolly
Tomy
Kitty
Spike
5. ________ formulated rule to explain the phenomenon of inheritance.
Gregor Johann Mendel
Charles Darwin
Jean Baptiste de Lamark
All of them
6. Each basis inheritable character is controlled by a factor called ________.
Chromosome
DNA
RNA
gene
7. Gene is made up of ________.
Proteins
RNA
DNA
All of them
8. The discovery of ________ was a tremendous stimulus to genetic research.
DNA of bacteria
cloned Dolly
Role of DNA
genetic engineering
9. Methodology was discovered in ________.
1952
1956
1973
1989
10. Methodological methods were referd to as ________.
Recombinant DNA technology
Genetic engineering
Both
All of them
11. ________ is defined as the “se of living organisms for the welfare of mankind”.
Micro-biology
Human biology
Bbiotechnology
Zoology
12. Genetic engineering usually utilizes ________.
Bacterial cells
Bacterial plasmids
Both of them
None of them
13. Recombinant DNA technology depends on ________ steps.
3
4
5
8
14. A gene is inserted into a DNA molecule called ________.
vector
pasmid
both
none of them
15. ________ are small, extra circular DNAs molecules found in some bacteria.
vector
plasmid
chromosome
genetic engineered DNA
16. Plasmid replicated ________ of the host cell.
dependent
independent
mostly dependent
mostly independent
17. Plasmids are generally found in ________.
bacteria
vertebrates
all living organisms
bacteriophages
18. The group of enzymes that are used to cut up the DNA molecule are called ________.
restriction enzymes
DNA ligase enzyme
DNA polymerase enzyme
none of them
19. ________ protest bacteria against bacteriophage by cutting viral DNA.
DNA polymerase enzyme
restriction enzyme
DNA ligase enzyme
all of them
20. The molecular scissors in the bacterial cell are ________.
DNA ligase enzyme
vector
plasmid
restriction enzyme
21. Each restriction enzyme cut the DNA at a special site called ________.
sticky end
site of attachment
cutting end
joining ends
22. ________ is a key enzyme that seals the restriction fragment with sticky ends of vector.
DNA polymerase enzyme
DNA ligase enzyme
Restriction enzyme
A and B
23. ________ are taken as host in DNA recombinant technology.
bacterial cells
vectors
plasmids
bacteriophage
24. The gene carried by recombinant molecule is called ________.
cloned
copied
multiplied
engineered
25. ________ is used to form genetically engineered plants which are resistant towards insects.
genetically engineered bacteria
vector
plasmid
all of them
26. Bacteria which usually live in the roots of corn are transformed for producing ________.
good roots
strong barks
insect killing foxins
healthy fruits
27. Hormones can also be prepared by ________.
cloned bacteria
plasmid
vector
rDNA
28. Phynylalanine is used to make a sweetener called ________.
phenyl sweets
nutra sweets
cemilky sweets
natural sweets
29. When a foreign gene is inserted to natural plants they are called ________.
Transgenic plants
genetically engineered plants
both A and B
all of them
30. In ________ technique entire organism can be grown from a single call.
tissue culture
cloning
genetic engineering
transfusion
31. When the cell wall of a plant cell is removed it is called ________.
protoplast
plant cell
changed cell
transgenic cell
32. In plant cell the rDNA can be introduced into ________.
Enbryo
Protoplast
Cell having cell wall
Both A and B
33. The only plasmid for transgenic plants is ________.
Pr-plasmid
Ti-plasmid
Ds-plasmid
All of them
34. ________ is the host for the host for the Ti-plasmid.
Bacteriophage
Agrobacterium
Phenylalamine
All bacteria
35. Transgenic plants are able to make use of ________ of the atmosphere so they dont require fertilizers.
Oxygen
Nitrogen
CO2
All of them
36. ________ is the fastest way of determining amino acid sequences.
DNA finger printing
Gene sequencing
Genomic library
Human genome project
37. ________ provides the location of Restriction site within the gene.
Gene sequencing
DNA finger printing
Genomic library
All of them
38. The similarity in the DNAs of two different organisms can be determined and studied by ________.
DNA finger printing
Human genome project
Gene sequencing
Gene therapy
39. ________ can separate DNA fragments differing in as much as 1 segment in gene sequencing.
Gel electrophoreses
DNA electrophoreses
Gene electrophoreses
All of them
40. ________% of DNA does not code for proteins
20%
30%
40%
50%
41. The difference in DNA electrophoresis patterns among individuals is called ________.
DNA finger prints
DNA fragment length
DNA length measurement
Restriction fragment length polymorphism
42. DNA finger prints is named so due to ________
RFLPs length
Sequence of RFLPs is unique for each
Both A and B
None of the above
43. To produce a DNA finger print ________ would suffice.
A sample of blood
A sample of saliva
Sample of hair folicle
All of them
44. ________ consists of the copy of genetic information or a specre the prefered environment.
Genome library
DNA finger prints
DNA fragment
Gene sequencing
45. Human Genome Project began in ________.
1990
1980
1994
1991
46. Total number of chromosome pairs inhuman is ________.
23
26
44
46
47. ________% of the DNA sequence have determined with 99% accuracy.
89%
95.658%
99.335%
99.99%
48. ________% of the nucleotide sequence in all the hamans is same.
50%
86%
99%
99.9%
49. Medical researches have discovered about ________ genetic diseases.
3000
4000
5000
6000
50. Genetic diseases is mostly related with ________.
Sperms
Ova
Fertilized egg or zygote
All of them
51. There are ________ conditions for the occurance of genetic diseases.
2
3
5
9
52. Is mutation a cause of genetic diseases?
Yes always
No
May be
Not involved at all
53. Genetic diseases fall into ________ main categories.
2
3
5
8
54. Following is the category of genetic diseases.
Chromosomal abnormalities
Unifactorial defects
Multifactorial defects
All of them
55. Huntington’s disease is due to a ________.
Autosomal resesive allele
Autosomal dominant allele
Hetyerozygous allele
Homozyhous allele
56. In huntington’s disease the symptoms appear by the age of ________.
35-45
40-50
55-65
50-60
57. Progressive mental deterioration and unvoluntary muscle movement is the characteristic of ________.
Chromosomal disorder
Chromosomal disjunction
Huntington’s disease
Cystic fibrosis
58. Is a cure for huntington’s disease possible.
Yes
May be
Depends on nature
No
59. Cystic fibrosis occurs due to ________
Autosomal Recessive Allele
A heterotroph
Homozygous Chromosome
Dominant Allele
60. ________ is a diagnostic process in which man amount of amniotic fluid is taken from the amniotic sac to
determine the presence of disease.
Genetic councelling
Tissue culture
Gene therapy
Amniocentesis
61. Through ________ any disease can be determined in the embryonic stage.
Amniocentesis
Amniotic tissue test
fefune blood test
Gene therapy
62. ________ is a test tube method to create and olone various varieties of novel plants.
Cloning
Genetic engineering
Gene therapy
Tissue culture
63. The cell which contains all the genetic potential of the organism is called ________.
Potitotent
Totipotent
Sturgid call
Complete cell
64. Through cloning the duplicate coples of ________ can be formed.
Genetic material
Cells
Both of them
None of them
65. Artificial cloning is now possible by the pethology ________.
Recombinant DNA technology
Gene therapy
DNA finger prints
Genomic library
66. The copies of individuals formed by clonig are called ________.
Twins
Identicals
Clones
Copies
67. In 1997, a ________ was cloned from cell from its mother.
Cat
Dog
Fish
Sheep
68. The biotechnology is now also being considered dangerous due to
Its useful cloning method
DNA finger printing
Harmful side effects
Artificial Techniques
69. In gene therapy of germ line cells the recombinant DNA is inserted into ________.
Human sex cells
Fertilized zygote
Somatic cells
All of them