BIOTECHNOLOGY AND SUSTAINABLE AGRICULTURE 2006

AND BEYOND

and

Edited by

Biotechnology and Sustainable

Agriculture 2006 and Beyond

August 31 18, 2006 Beijing, China

Zhihong XuPeking University, China

Jiayang Li

Chinese Academy of Sciences, Beijing, China

Yongbiao Xue

Chinese Academy of Sciences, Beijing, China

Chinese Academy of Sciences, Beijing, China

Weicai Yang

Institute of Genetics and Developmental Biology,

Institute of Genetics and Developmental Biology,

Institute of Genetics and Developmental Biology,

-Proceedings of the 11th IAPTC&B Congress,

A C.I.P. Catalogue record for this book is available from the Library of Congress.

Published by Springer,

www.springer.com

Printed on acid-free paper

All Rights Reserved

No part of this work may be reproduced, stored in a retrieval system, or transmitted

and executed on a computer system, for exclusive use by the purchaser of the work.

P.O. Box 17, 3300 AA Dordrecht, The Netherlands.

© 2007 Springer

exception of any material supplied specifically for the purpose of being entered

in any form or by any means, electronic, mechanical, photocopying, microfilming,

recording or otherwise, without written permission from the Publisher, with the

ISBN 978-1-4020-6634-4 (HB)ISBN 978-1-4020-6635-1 (e-book)

v

CONTENTS

PLENARY LECTURES

Zhihong Xu Facing the Future with Pharmaceuticals from Plants…………………….13 Rainer Fischer, Richard M. Twyman, Stephan Hellwig,

Embryogenesis In Vitro…………………………………………………..35 Jim M. Dunwell

Multigene Strategies for Engineering the Phytoremediation of Mercury and Arsenic…………………………………………………..49 Richard B. Meagher, Aaron P. Smith, Melissa Pischke, Tehryung Kim, Om Prakash Dhankher, and Andrew C.P. Heaton

Molecular Control of S-RNase-based Self-Incompatibility……………...63 Jian Huang, Lan Zhao, and Yongbiao Xue The Use of Sexual Model Systems to Identify Elements of Apomixis………………………………………………………………77 Ueli Grossniklaus, Philippa Barrell, Amal Johnston, Ramaurthy Baskar, Jacqueline Gheyselinck, Lukas Brand, and Mark Curtis Agricultural Biotechnology in China: Past, Present and Future………………………………………………………………...81 Valerie J. Karplus and Xing Wang Deng

Plant Biotechnology and Crop Improvement in China……………………3

2006 Science and Technology Exhibitors…............………………….…xix

Opening Speech by Zhihong Xu, President of the IAPTC&B..………....xxi

Jürgen Drossard, and Stefan Schillberg

vi Contents

SYMPOSIA

Current Advances in Plant Tissue Culture Techniques

Regulation of Plant Morphogenesis In Vitro: Role of Ethylene and Polyamines………………………………..……….89 Eng-Chong Pua

Lingfasu and Its Use on Plant Tissue Culture In Vitro……………..…....97

for Plum Pox Potyvirus Resistance……………………………..……….103

Antonet Svircev, Daniel C. Brown, and Rui Wen

An Efficient Novel Method of Producing Virus Free

Site-Directed Homologous Recombination in Tobacco

Charles Q. Cai, W. Michael Ainley, Trevor N. Collingwood, Robbi J. Garrison, Lisa L. Schulenberg, Andrew Worden, Philip D. Gregory, Beth C. Rubin-Wilson, and Joseph F. Petolino

Generation of Genetically Modified Strawberries in Temporary Immersion Bioreactor System…………………….……..115 Kati Hanhineva, Harri Kokko, and Sirpa Karenlampi

Current Advances in Plant Molecular Biology

The Positioning of the Division Plane Is Determined by a Cortical Division Site at the Plasma Membrane…………………...121 Danny Geelen

Repression of Plant Gene Expression via Chromosomal Remodelling Using Histone Deacetylases………………………………125 Lining Tian, Keqiang Wu, Brian Miki, and Dan Brown

Xu Hong-Yuan, Xu Hong-Zhang, He Bing, and Zhou Feng-Jue

Cell Cultures via Zinc Finger Nucleases………………………..………111

Plants from Garlic Root Meristem…………………..………………….107 Md. Shahidul Haque, K. Hattori, A. Suzuki, and T. Tsuneyoshi

Lining Tian, Shuocheng Zhang, HJIPne SanfaHon,

PPV-Specific Hairpin RNAs is an Effective Method

Contents vii

Functional Genomics

Ming Luo, Mingzhu Luo, Fred Berger, E.S. Dennis, Jim W. Peacock, and Abed Chaudhury

Integrating Transcriptional and Metabolic Profiling to Unravel

Heiko Rischer, Matej Oresic, Alain Goossens, Dirk Inzé, and Kirsi-Marja Oksman-Caldentey

Plant Transformation Technology

Fang-Ming Lai, Kangfeng Mei, Luke Mankin, and Todd Jones

Goetz Hensel, Vladimir Valkov, Conny Marthe, and Jochen Kumlehn

in Molecular Biology

Use of Alfalfa In Vitro Cultures in Studies on Regulation of Cyclin-Dependent Kinase (CDK) Functions……………………..…..149 Dénes Dudits, Mátyás Cserháti, Pál Miskolczi, Attila Fehér, Ferhan Ayaydin, and Gábor V. Horváth

Phytohormone Regulation of Cotton Fiber Development In Vitro……...153 Barbara A. Triplett, Hee Jin Kim, Doug Hinchliffe, Sing-Hoi Sze, Peggy Thaxton, David Stelly, and Z. Jeffrey Chen

Patterns of Growth and Cell Death in Diploid Arabidopsis Cell Cultures with Synchronised Cell Cycle……………………............157 Ranjith Pathirana, Phillip West, and Jocelyn Eason

Efficient Agrobacterium-Mediated Transformation of Various Barley (Hordeum vulgare L.) Genotypes……………..……..143

Application of Two New Selectable Marker Genes,

DNA-METHYLTRANSFERASE 1 is a Member of FIS Polycomb

In vitro System as the Model for Discovery

Complex and is Involved in Seed Development in Arabidopsis...............131

Secondary Metabolite Biosynthesis in Plants………………………...…135

dsdA and dao1 in Maize Transformation……………………………..….141

viii Contents

Molecular Control of Abiotic Resistance

Engineering of Reactive Species Detoxification Pathways for Increasing Stress Tolerance in Plants…………………………..……163 Gábor V. Horváth, Zoltán Turóczy, Mátyás Cserháti, Petra Kis, Katalin Török, László Sass, and Dénes Dudits

Orchid Flower Fragrance Biosynthesis Pathway and Its Key Enzyme Geranyl Diphosphate Synthase…………………………..…….167 Yu-Yun Hsiao, Wen-Chieh Tsai, Chang-Sheng Kuoh, Tian-Hsiang Huang, Hei-Chia Wang, Yann-Lii Leu, Tian-Shung Wu, Wen-Huei Chen, and Hong-Hwa Chen

Cytokinin Function in Drought Stress Response and Subsequent Recovery……………………………………..………...171 M. Novakova, P. Dobrev, V. Motyka, A. Gaudinova, J. Malbeck, J. Pospisilova, D. Haisel, H. Storchova,

Xenogenomics: Bioprospecting for Genetic Determinants of Cold and Freezing Stress Tolerance in the Cryophilic Antarctic Hair Grass Deschampsia antarctica E. Desv. …………..……175

D. Gunawardana, and G.C. Spangenberg

Plant Insect Interaction

P.E. Urwin

Molecular Basis of Colorado Potato Beetle Adaptation

N. Slapar, M. Pompe-Novak, M. Buh, M. Ravnikar, B. Štrukelj, M.A. Jongsma, and K. Gruden

Molecular Mechanisms on Plant-Microbe Interactions

An Arabidopsis Nudix Hydrolase Modulates the Innate Immune Response Independent of NPR1 and Salicylate………….……199 Yiji Xia, Xiaochun Ge, Guo-Jing Li, and Huifen Zhu

J. Dobra, M.C. Mok, D.W.S. Mok, R. Martin, and R. Vankova

M.J. Kuiper, J.P. Talbot, O. Chew, E. Ribarev, D. Azria,

Biotechnological Control of Plant Parasitic Nematodes………...............181

to Potato Defence Mechanisms………………………………..........…...191

U.P. John, R.M. Polotnianka, A. Sivakumaran, L. Mackin,

Contents ix

RNA-Mediated Resistance to Cassava Geminiviruses in Transgenic Cassava…………………………………………...………201 Hervé Vanderschuren, Rashid Akbergenov, Mikhail M. Pooggin, Thomas Hohn, Wilhelm Gruissem, and Peng Zhang

The Molecular Basis of Cold-Induced Pollen Sterility in Rice………....205 Sandra N. Oliver, Xiaochun Zhao, Elizabeth S. Dennis, and Rudy Dolferus

Regulation of the Rice NADPH Oxidase by Binding of Small GTPase Rac and Ca2+ to Its N-terminal Extension……………………...209 Hann Ling Wong, Reinhard Pinontoan, Kana Hasegawa, Takashi Yaeno, Koh Iba, Ryo Tabata, Kokoro Hayashi,

Chojiro Kojima, Tsutomu Kawasaki, and Ko Shimamoto

Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza Virus Confers Resistance to the Virus in Transgenic Mexican Lime…………………………..…...211 Carmen Fagoaga, Carmelo López, Alfonso Hermoso de Mendoza, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña

Phloem Feeding Regulates the Plant Defense Pathways Responding to Both Aphid Infestation and Pathogen Infection………...215 Yinghua Huang

Metabolic Engineering

Towards Metabolic Engineering of Carotenoid Content in Sweet-orange (Citrus sinensis (L.) Osb.)………………………..…...223 Marcio G.C. Costa, Amanda F.S. Mendes, Luciana C. Cidade, Walter S. Soares-Filho, Wagner C. Otoni, and Gloria A. Moore

Engineering New Crops for Safe Castor Oil Production………..............227 Grace Q. Chen, Yeh-Jin Ahn, and Louisa Vang

Biochemical and Structural Bases for (Iso)flavonoid Biosynthetic Diversity………………………………………..…………231 Chang-Jun Liu, Wang Cheng, Joseph P. Noel, and Richard A. Dixon

x Contents

Utilization of Plant Metabolic Engineering for Production of Pharmaceuticals in Tea…………………………………………….....235 A. Borthakur, R.K. Dutta, S. Borchetia, and Sudripta Das

Improving Nutritional Quality of Crops

Success Towards Alleviating Peanut Allergy: The Major Allergen Ara h 2 is Silenced via RNA Interference (RNAi)………….…261 K.N. Konan, O.M. Viquez, F.C. Chen, and H.W. Dodo

Transgenic Poplar for Phytoremediation………………..………………265 Won-Yong Song, Young-Im Choi, Donghwan Shim, Do-Young Kim, Eun-Woon Noh, Enrico Martinoia, and Youngsook Lee

The Africa Biofortified Sorghum Project – Applying Biotechnology to Develop Nutritionally Improved Sorghum for Africa……………………………………………………..…………273 Zuo-yu Zhao

Genetic Engineering of Strawberry for Taste Improvement and Enhanced Disease Resistance by Introduction of thauII Gene………….279

Genetic Engineering of Crops for Improved Nutritional Quality……….283 Samuel S.M. Sun, Qiaoquan Liu, and Rebecca M.L. Chan

Transcriptome Profiling of Wheat Genotypes Differing in Bread Baking Quality under Different Environments……………..…289 Yongfang Wan, Rowan Mitchell, Tim Wheeler, Mike Gooding,

Salvador Gezan, Sue Welham, Christina Shenton, Tong Zhu, and Peter Shewry

Signal Transduction

Cotton 14-3-3L Gene Is Preferentially Expressed in Fiber……….….…295 Xue-Bao Li, Hai-Yan Shi, Xiu-Lan Wang, Wen-Liang Xu, Deng-Di Li, and Hong Wang

Rebecca Poole, Keith Edwards, Clare Mills, Peter Skeggs,

K.A. Schestibratov, and S.V. Dolgov

Contents xi

Inositol Polyphosphate 6-/3-kinase (AtIpk2β), an Early Auxin-Responsive Gene, Positively Regulates Axillary Shoot Branching in Arabidopsis thaliana...........................................................299

and Hui-Jun Xia

Apomixis

Genetic Analyses of Aposporous Embryo Sac Formation in Sorghum………………………………………………………………305 John G. Carman, Michelle S. Jamison, Jayasree Pattanayak, Jeff Lacey, Jeong-Soon Kim, Estella G. Elliott, Patricia Klein, Tom Ulrich, and Krishna Dwivedi

Epigenetic Regulation of Seed Development………………..……….…309 Claudia Köhler

Biotechnology in Cereals

Optimisation of Tissue Culture, Regeneration and Agrobacterium-Mediated Transformation Paramaters in Winter Wheat Cultivars (T.durum cv. K z ltan-91

M. Kavas; H.A. Öktem, and M. Yücel

Enhancement of the Brassinosteroid Biosynthesis Pathway

Chuan-Yin Wu, Shing Kwok, Sam Harris, Anthony Trieu, Parthiban Radhakrishnan, Andres Salazar, Ke Zhang, Jiulin Wang, Jianmin Wan, Shozo Fujioka, Ken Feldmann, and Roger Pennell

Yasunari Ogihara

Zai-Bao Zhang, Guang Yang, Zhen Chen, Yan Li, Fernando Arana,

ı ı

Biotechnology in Economic Crops

Isolation and Characterization of Senescence-Associated

and Helen Nair Zuliana Razali, S. Chandran, Lee Ai Ling, A.N. Boyce,

and T.aestivum cv. Bezostaja-01).............................................................315

Improves Grain Yield in Rice………………………………………..….319

Functional Genomics of Hexaploid Wheat…………………….………..323

Ethylene Genes from Dendrobium Orchids………………………..……327

xii Contents

Zeng-Yu Wang, Jeremey Bell, Xiaofei Cheng, Yaxin Ge, Kun Jun Han, Xuefeng Ma, Elane Wright, Yajun Xi, Xirong Xiao, Ji-Yi Zhang, Andrew Hopkins, and Joseph Bouton

Biotechnology in Medicinal, Woody and Horticultural Plants

Toshiaki Umezawa

Polyketide Metabolism in Hypericum perforatum

Ludger Beerhues and Benye Liu

In Vitro Selection and Molecular Markers for Early

F. Eudes, Ana Badea, A. Laroche, D. Gaudet, R. Graf, and S. Sadasivaiah

The Geo-Model for Cell Wall Formation: A Design Tool

Carolina Cifuentes and Anne Mie C. Emons

Tissue Culture for Biodiversity Preservation

Integrating In Vitro Methods for Propagating and Preserving

V.C. Pence, S.M. Charls, B.L. Plair, M.A. Jaskowiak, G.D. Winget, and L.L. Cleveland

Micropropagation and Germplasm Conservation of Staple

Virendra Mohan Verma

Efficient In Vitro Bulblet Regeneration from Immature Embryos of Endemic and Endangered Geophyte Species

Sebahattin Özcan, İskender Parmaksız, Semra Mirici,

and Neşet Arslan a

Satı Çöçü, Serkan Uranbey, Arif İpek, Cengiz Sancak, Erc ment O. S rıhan, Bilal Gürbüz, Cafer S. Sevimay, ü

Biotechnological Improvement of Forage Crops…………………..……333

Lignan and Norlignan Biosynthesis and Biotechnology………...………341

and Related Species…………………………………………..……...….345

Screening of Fusarium Head Blight Resistance Wheat………..………...349

for Plants……………………………………………………………...…353

Endangered Plants……………………………………………………....363

Food Crops and Traditional Medicinal Plants in Micronesia………..….375

in Sternbergia, Muscari and Fritillaria Genera……………………...…381

Contents xiii

Pramod Tandon

Plants as Bioreacter/Molecular Pharming

Elisabetta Lupotto and Maria Rosaria Stile

Somika Bhatnagar, Yuan Qing Deborah Hong,

Production of Recombinant Antibodies in Pea Seeds

Isolde Saalbach, Marcus Riehl, Martin Giersberg, Jochen Kumlehn, and Dieter Falkenburg

Molecular Breeding

Farhad Nazarian Firouzabadi, Geraldine Kok-Jacon,

Kirsten Jørgensen, Charlotte Sørensen, Susanne Jensen, Marc Morant, Martin Fregene, and Birger Lindberg Møller

Evaluation of GM Poplars Expressing Relevant Traits for Herbicide Tolerance, Disease Resistance and Production of Pharmaceutics: Biochemical, Molecular and Microbiological Studies on Plants

F. Mattivi, M. Bonadei, M. Gennaro, E. Quattrini, P. Calligari, F. Picco, G. Deandrea, and A. Balestrazzi

Ji Qin, Jean-Paul Vincken, Luc Suurs, and Richard G.F. Visser

Guang Yuan Wang, and Yan Hong

S. Zelasco, D. Carbonera, A. Giorcelli, M. Confalonieri,

Molecular Breeding of Low-Phytic-Acid Grains in Rice

Mio Kuwano, Makoto Suzuki, Fumio Takaiwa, and Kaoru T. Yoshida

In Vitro Strategies for Conservation of Plant Diversity in India……..…385

Molecular Farming in Plants: An Update……………………..………....389

In Vivo and In Vitro Studies on Huperzine A Producing Ferns…………397

and Their Oral Application in Piglets………………………..……….…399

Breeding for Improved and Novel Starch Characteristics in Potato...…..405

Biofortification of Cassava Using Molecular Breeding…………..….….409

and Detection of Transgene Sequences in Soil………………...........…. 413

by Using the Promoter of 18 kDa Oleosin………………………...…….419

xiv Contents

Regeneration, Micropropagation and Industrialization

A Highly Efficient Protocol for Micropropagation

Sijun Zhou and Daniel C.W. Brown

The Use of Tissue Culture for Successful

Satjaporn Chantawong, Upsorn Pliansinchai, Chalermpol Kirdmanee, and Pipat Weerathaworn

Development of Subclonal Variants from Interspecific

S. Rajeswari, Shini Sekar, and M. Krishnamurthi

Forcing and In Vitro Establishment of Softwood Shoots

Faheem Aftab and John E. Preece

Protoplasts, Haploids and Embryo Culture in Crop Improvement

Introgression Breeding Program in Lolium/Festuca

Toshihiko Yamada, Yang-dong Guo, Yuuko Mizukami, Ken-ichi Tamura, and Kazuhiro Tase

Recent Advances in Anther Culture of Coconut

P.I.P. Perera, V. Hocher, J.L. Verdeil, D.M.D. Yakandawala, and L.K. Weerakoon

Protoplast Isolation and Cultivation from Embryogenic

Jiang Lu, Xia Xu, and Judy Grosser

W.W. Guo, X.D. Cai, Y.J. Cheng, J.W. Grosser, and X.X. Deng

of North American Ginseng……………………………………………..425

and Sustainable Cane………………………………………..……..……429

Hybrids of Sugarcane……………………………………………..…….433

from Large Stem Segments of Woody Plants……………………..…....437

Complex Using Androgenesis…………………………………..………447

(Cocos nucifera L.)…………………………………………..…….……451

Suspension Cultures and Leaves of Grapevines (Vitis L.)……………….457

Protoplast Technology and Citrus Improvement…………..…………....461

Contents xv

A.M.R. Ferrie and T.D. Bethune

Integration of Cold Signal Transduction Pathway Related

Xin Zhou, Fei He, Fengxia Liu, Xue Zheng, Chao Di, Shaoxia Zhou, Hongya Gu, Wenying Xu, and Zhen Su

Rice Genetics and Genomics Information as the Ultimate

Takuji Sasaki, Jianzhong Wu, and Takashi Matsumoto

The Development of Agricultural Biotechnology Capacities in Palestine through the UNESCO Biotechnology Educational

Naim Iraki, Omar Dar-Issa, Basma Sandouka, Michael Sansour, Rami Arafeh, Amani Abu-Sa’da, and Nida’ Salah

Technology Transfer to Developing Countries

Microspore Embryogenesis in the Apiaceae………………..…………...465

to ABA 8’-hydroxylase in Arabidopsis…………………….………..…..471

Tool for Rice Improvement………………………………………...……475

and Training Center at Bethlehem University………………….……….487

Author Index………………………………………………..………….491

xvii

Komamine, Yongbiao Xue (Chair-Local Organizing Commitee, Co-Editorof In Vitro Plant), Eng-Chong Pua (Editor of In Vitro Plant).

From left: Arie Altman, Zhihong Xu (President), Indra K. Vasil, Atsushi

xviii

IAPTC&B President Zhihong Xu announcing the election of Dr. Roger Beachy of USA as President of IAPB for 2006–2010, and of St. Louis as the host city of the 12th Congress in 2010.

Indra K. Vasil on behalf of President Roger Beachy of 12th IAPB (2006–2010) receive the logo from President Zhihong Xu of 2002–2006 of IAPTC&B.

Alto Holdings Limited, New Zealand DBN Group, China Duchefa Biochemie bv, The Netherlands Eppendorf China Ltd., China Phyto Technology Laborateries, USA Sigma-Aldrich Trading Co. Ltd., China Springer, Germany Sunshine Horticulture Co., Ltd., China Yantze Deltaregion Institute of Tsinghua University, Zhejiang, China

2006 Science and Technology Exhibitors

xix

Opening Speech by Zhihong Xu,

President of IAPTC&B 2006

Good Morning, Ladies and Gentlemen!

It has been 4 years since we met in Orlando, USA in June, 2002.

On behalf of the International Association for Plant Tissue Culture and Biotechnology, I would like to express my warm welcome to all the delegates attending the 11th International Congress of Plant Tissue Culture and Biotechnology.

This Congress is certainly a world-class event with over 800 parti-cipants from 94 countries. In the following 5 days, the best and the brightest of international scientific leaders will present 14 plenary, 34 keynote and 120 symposia lectures covering the entire research field of plant biotechnology, as well as laws and policies regarding trans-genic plants and technology transfer. More than 600 posters will augment the formal lecture sessions. The IAPTC&B awarded 15 fellowships to graduate students and post-doctoral associates from developing countries.

The International Association for Plant Tissue Culture and Bio-technology is one of major international professional associations in the field of life science. The mission of the Association is to promote research in all aspects of basic and applied research in plant tissue culture and biotechnology. The theme of this Congress is ‘Biotech-nology and Sustainable Agriculture’. It is a theme that is especially timely for plant biologists working today. I hope that this Congress will further promote global research activities in the field of plant biotechnology and thus make greater contributions to the world people.

Besides scientific activities in the Congress, I hope you will also enjoy your stay in Beijing. Beijing is one of the most attractive and cosmopolitan cities in the world. It is the political and cultural center of ever-growing China, home of 11 million citizens, seat of the Chinese government, a city highly celebrated for its beautiful surroun-dings and well-preserved historic sites and cultural relics. I would

xxi

like to suggest that you enjoy your hot summer by visiting the Summer Palace where the emperors enjoyed their hot summer in old days.

Finally, I wish the Congress success, and all of you a pleasant stay in Beijing.

Speech Openingxxii